Annual National Report

ZOONOSES MONITORING

Greece

TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS

including information on foodborne outbreaks, antimicrobial resistance in zoonotic and indicator bacteria and some pathogenic microbiological agents

IN 2019

Greece - 2019 1 Greece - 2019 Report on trends and sources of zoonoses

PREFACE

This report is submitted to the European Commission in accordance with Article 9 of Council Directive 2003/99/ EC*. The information has also been forwarded to the European Food Safety Authority (EFSA).

The report contains information on trends and sources of zoonoses and zoonotic agents in Greece during the year 2019.

The information covers the occurrence of these diseases and agents in animals, foodstuffs and in some cases also in feedingstuffs. In addition the report includes data on antimicrobial resistance in some zoonotic agents and indicator bacteria as well as information on epidemiological investigations of foodborne outbreaks. Complementary data on susceptible animal populations in the country is also given. The information given covers both zoonoses that are important for the public health in the whole European Union as well as zoonoses, which are relevant on the basis of the national epidemiological situation. The report describes the monitoring systems in place and the prevention and control strategies applied in the country. For some zoonoses this monitoring is based on legal requirements laid down by the European Union legislation, while for the other zoonoses national approaches are applied.

The report presents the results of the examinations carried out in the reporting year. A national evaluation of the epidemiological situation, with special reference to trends and sources of zoonotic infections, is given. Whenever possible, the relevance of findings in foodstuffs and animals to zoonoses cases in humans is evaluated. The information covered by this report is used in the annual European Union Summary Reports on zoonoses and antimicrobial resistance that are published each year by EFSA.

The national report contains two parts: tables summarising data reported in the Data Collection Framework and the related text forms. The text forms were sent by email as pdf files and they are incorporated at the end of the report.

* Directive 2003/ 99/ EC of the European Parliament and of the Council of 12 December 2003 on the monitoring of zoonoses and zoonotic agents, amending Decision 90/ 424/ EEC and repealing Council Directive 92/ 117/ EEC, OJ L 325, 17.11.2003, p. 31

Greece - 2019 2 List of Contents

ANIMAL POPULATION TABLES 3 DISEASE STATUS TABLES FOR BRUCELLA 4 Bovine brucellosis in countries and regions that do not receive Community co-financing for eradication programme 4 Ovine or Caprine brucellosis - data on animals - Community co-financed eradication programmes 5 Ovine or Caprine brucellosis - data on herds - Community co-financed eradication programmes 6 Ovine or Caprine brucellosis - data on status of herds at the end of the period - Community co-financed eradication programmes 7 DISEASE STATUS TABLES FOR MYCOBACTERIUM 8 Bovine tuberculosis in countries and regions that do not receive Community co-financing for eradication programme 8 PREVALENCE TABLES 9 Bacillus:BACILLUS 9 animal 9 Campylobacter:CAMPYLOBACTER 10 animal 10 food 11 Chlamydia/ Chlamydophila:CHLAMYDIA/ CHLAMYDOPHILA 12 animal 12 Clostridium:CLOSTRIDIUM 13 animal 13 COXIELLA 14 animal 14 Echinococcus:ECHINOCOCCUS 15 animal 15 Escherichia coli:ESCHERICHIA COLI 16 animal 16 food 17 feed 20 Escherichia coli, non-pathogenic:ESCHERICHIA COLI, NON-PATHOGENIC 21 animal 21 FLAVIVIRUS 22 animal 22 Klebsiella:KLEBSIELLA 27 animal 27 Leishmania:LEISHMANIA 28 animal 28 Listeria:LISTERIA 29 animal 29 food 30 Lyssavirus:LYSSAVIRUS 33 animal 33 Proteus:PROTEUS 36 animal 36 Salmonella:SALMONELLA 37 animal 37 food 39 feed 44 Staphylococcal enterotoxins:STAPHYLOCOCCAL ENTEROTOXINS 45 food 45 Staphylococcus:STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) 46 animal 46 food 47 Streptococcus:STREPTOCOCCUS 49 animal 49 Toxoplasma:TOXOPLASMA 50 animal 50 Trichinella:TRICHINELLA 51 animal 51 FOODBORNE OUTBREAKS TABLES 52 AMR TABLES FOR CAMPYLOBACTER 55 AMR TABLES FOR SALMONELLA 56 Salmonella Adabraka 56 Meat from pig - carcase - Slaughterhouse - Surveillance - based on Regulation 2073 - Official sampling - AMR MON 56 N_A 56 Salmonella Bareilly 57 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 57 N_A 57 Salmonella Bovismorbificans 58 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 58 N_A 58 Salmonella Braenderup 59 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 59 N_A 59 Salmonella Bredeney 60 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 60 N_A 60 Salmonella Cerro 61 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 61 N_A 61 Salmonella Enteritidis 62 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 62 N_A 62 Salmonella group C1 63 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 63 N_A 63 Salmonella Herston 64 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 64 N_A 64 Salmonella Infantis 65 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 65 N_A 65 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 66 N_A 66 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 67 N_A 67 Salmonella Kedougou 68 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 68 N_A 68 Salmonella Kentucky 69 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 69 N_A 69 Salmonella Kottbus 70 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 70 N_A 70 Salmonella Livingstone 71 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 71 N_A 71 Salmonella Mishmarhaemek 72 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 72 N_A 72 Salmonella Montevideo 73 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 73 N_A 73 Salmonella Muenchen 74 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 74 N_A 74 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 75 N_A 75 Salmonella Muenster 76 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 76 N_A 76 Salmonella Senftenberg 77 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 77 N_A 77 Salmonella Szentes 78 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - HACCP and own check - AMR MON 78 N_A 78 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 79 N_A 79 Salmonella Thompson 80 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 80 N_A 80 Salmonella Typhimurium, monophasic 81 Meat from pig - carcase - Slaughterhouse - Surveillance - based on Regulation 2073 - Official sampling - AMR MON 81 N_A 81 Salmonella Umbilo 82 Gallus gallus (fowl) - broilers - Farm - Control and eradication programmes - Official sampling - AMR MON 82 N_A 82 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 83 N_A 83 Salmonella Uno 84 Gallus gallus (fowl) - laying hens - Farm - Control and eradication programmes - Official sampling - AMR MON 84 N_A 84 AMR TABLES FOR ESCHERICHIA COLI 85 Escherichia coli, non-pathogenic, unspecified 85 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 85 N_A 85 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON 86 N_A 86 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 87 N_A 87 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON 88 N_A 88 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 89 N_A 89 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON 90 N_A 90 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 91 N_A 91 Meat from bovine animals - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON 92 N_A 92 Pigs - fattening pigs - Slaughterhouse - Monitoring - EFSA specifications - Official sampling - AMR MON pnl2 93 N_A 93 Pigs - fattening pigs - Slaughterhouse - Monitoring - EFSA specifications - Official sampling - AMR MON 94 N_A 94 Pigs - fattening pigs - Slaughterhouse - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 96 N_A 96 Pigs - fattening pigs - Slaughterhouse - Monitoring - EFSA specifications - Official sampling - ESBL MON 98 N_A 98 Meat from pig - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 100 N_A 100 Meat from pig - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON 101 N_A 101 Meat from pig - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON pnl2 102 N_A 102 Meat from pig - fresh - Retail - Monitoring - EFSA specifications - Official sampling - ESBL MON 104 N_A 104 OTHER AMR TABLES 106 ESBL 107 LATEST TRANSMISSIONS 109 ANIMAL POPULATION TABLES

Table Susceptible animal population

Metrics Population slaughter animal Animal species Category of animals Unit holding animal (heads) herd/flock Cattle (bovine animals) Cattle (bovine animals) 805,357 134,163 18,003 Deer Deer - farmed 865 17 Ducks Ducks 5,975 Fish Fish 11 70 Fish - farmed - carp 7 10 Fish - farmed - salmon 2 3 Gallus gallus (fowl) Gallus gallus (fowl) - breeding flocks, unspecified 84 2,152,443 331 Gallus gallus (fowl) - broilers 1,065 112,795,034 8,893 Gallus gallus (fowl) - laying hens 629 6,374,300 983 Geese Geese 373 5,189 Minks Minks - farmed 475,000 93 Pigs Pigs 407,093 1,178,764 1,560 Rabbits Rabbits - farmed 31,581 703 Sheep and goats Sheep and goats 15,010,594 4,442,716 76,222 Shellfish Shellfish - farmed 590 23,500 Solipeds, domestic Solipeds, domestic 21,879 8,706 Turkeys Turkeys - breeding flocks, unspecified 8 14,500 10 Turkeys - meat production flocks 27 339,560 86 Wild boars Wild boars - farmed 3,171 73

Greece - 2019 3 DISEASE STATUS TABLES

Table Bovine brucellosis in countries and regions that do not receive Community co-financing for eradication programme

Metrics Number of notified abortions Number of Number of Number of whatever suspended Number of Number of animals or infected cause herds under Number of Number of Number of infected herds pools herds under investigatio herds with herds animals herds tested tested tested investigatio ns of status Number of tested tested Total tested under under under ns of suspect officially infected under under number of under surveillance surveillance surveillance suspect Region cases free herds surveillance surveillance herds surveillance by bulk milk by bulk milk by bulk milk cases GREECE(NUTS 1 4,114 85 3,420 150,977 10,726 102 1,425 109,473 14 1 2010-2013)

Greece - 2019 4 Table Ovine or Caprine brucellosis - data on animals - Community co-financed eradication programmes

Metrics Number of animals to Number of Number of Total Total be tested Number of animals Number of positive number of number of under the animals tested positive animals animals Region animals program tested individually animals slaughtered slaughtered GREECE(NUTS 5,770,543 5,770,543 198,555 198,555 63 61 61 2010-2013)

Greece - 2019 5 Table Ovine or Caprine brucellosis - data on herds - Community co-financed eradication programmes

Metrics Number of herds under Number of Number of the new Number of Total herds under program Number of positive depopulate number of the tested/chec positive Region herds d herds herds program ked herds GREECE(NUTS 9 0 22,188 22,188 1,109 37 2010-2013)

Greece - 2019 6 Table Ovine or Caprine brucellosis - data on status of herds at the end of the period - Community co-financed eradication programmes

Metrics Number of Number of Number of animals Number of animals Number of herds with with status herds with with status Number of animals status not not free or status not not free or herds with with status Total Total Number of free or not not free or not not status free free or Number of Number of number of number of Number of animals officially officially officially officially or officially officially herds with animals herds under animals herds with with free and free and free and free and free free status with status the under the unknown unknown last check last check last check last check suspended, suspended, officially officially program, at program, at status, at status, at positive, at positive, at negative, at negative, at at the end at the end free, at the free, at the the end of the end of the end of the end of the end of the end of the end of the end of of the of the end of the end of the Region the period the period the period the period the period the period the period the period period period period period GREECE(NUTS 42,188 5,770,543 28,298 1,886,296 25 10,890 977 353,384 9 1,285 365 103,616 2010-2013)

Greece - 2019 7 DISEASE STATUS TABLES

Table Bovine tuberculosis in countries and regions that do not receive Community co-financing for eradication programme

Metrics Number of animals with suspicious lesions of tuberculosis examined and submitted to Number of animals Number of animals histopathological and detected positive in Number of herds with Number of infected Total number of Interval between tested with tuberculin bacteriological bacteriological Region status officially free herds animals routine tuberculin tests routine testing examinations examination Total number of herds GREECE(NUTS 4,833 93 805,233 12 149,871 128 0 18,003 2010-2013)

Greece - 2019 8 PREVALENCE TABLES

Table Bacillus:BACILLUS in animal

Greece - 2019 9 Table Campylobacter:CAMPYLOBACTER in animal

Greece - 2019 10 Table Campylobacter:CAMPYLOBACTER in food

Greece - 2019 11 Table Chlamydia/ Chlamydophila:CHLAMYDIA/ CHLAMYDOPHILA in animal

Greece - 2019 12 Table Clostridium:CLOSTRIDIUM in animal

Greece - 2019 13 Table COXIELLA in animal

Total Total N of clinical Sampling units units affected Metrics N of units Area of Sampling Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling strategy unit Sampling Details Method tested positive herds Zoonoses positive GREECE(NUTS Cattle (bovine animals) - Farm - Greece - animal sample - blood - Clinical investigations - Official sampling - Suspect animal N_A Indirect ELISA 13 1 Coxiella burnetii 1 2010-2013) sampling (I-ELISA) Goats - Farm - Greece - animal sample - blood - Clinical investigations - Official sampling - Suspect sampling animal N_A Indirect ELISA 39 11 Coxiella burnetii 11 (I-ELISA) Sheep - Farm - Greece - animal sample - blood - Clinical investigations - Official sampling - Suspect sampling animal N_A Indirect ELISA 84 24 Coxiella burnetii 24 (I-ELISA) Sheep and goats - Farm - Greece - animal sample - blood - Clinical investigations - Official sampling - Suspect sampling animal N_A Indirect ELISA 40 6 Coxiella burnetii 6 (I-ELISA) Wild animals - Farm - Greece - animal sample - blood - Clinical investigations - Official sampling - Suspect sampling animal N_A Indirect ELISA 34 0 Coxiella burnetii 0 (I-ELISA)

Greece - 2019 14 Table Echinococcus:ECHINOCOCCUS in animal

Greece - 2019 15 Table Escherichia coli:ESCHERICHIA COLI in animal

Greece - 2019 16 Table Escherichia coli:ESCHERICHIA COLI in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Cheeses made from sheep's milk - hard - made from pasteurised milk - batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 2010-2013) Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official, based on Regulation 854/2004 - Objective d) sampling Cheeses made from sheep's milk - soft and semi-soft - Processing plant - batch 10 Gram N_A Not Available 10 0 Escherichia coli 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official, (food/fee based on Regulation 854/2004 - Objective sampling d) Cheeses, made from unspecified milk or other animal milk - hard - made batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 from pasteurised milk - Processing plant - Greece - food sample - (food/fee Surveillance - based on Regulation 2073 - Official, based on Regulation d) 854/2004 - Objective sampling Fruits and vegetables - pre-cut - Hospital or medical care facility - Greece batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) Live bivalve molluscs - Farm - Greece - food sample - Surveillance - based batch 50 Gram N_A Not Available 20 4 Escherichia coli 4 on Regulation 2073 - Official sampling - Objective sampling (food/fee d) Live bivalve molluscs - mussels - Farm - Greece - food sample - batch 50 Gram N_A Not Available 149 5 Escherichia coli 5 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d) Live bivalve molluscs - mussels - Natural habitat - Greece - food sample - batch 50 Gram N_A Not Available 2 0 Escherichia coli 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d) Live bivalve molluscs - Natural habitat - Greece - food sample - batch 25 Gram N_A Not Available 24 0 Escherichia coli 0 Surveillance - Official, based on Regulation 854/2004 - Objective sampling (food/fee d) Live bivalve molluscs - Packing centre - Greece - food sample - batch 25 Gram N_A Not Available 5 0 Escherichia coli 0 Surveillance - Official, based on Regulation 854/2004 - Objective sampling (food/fee d) Meat from bovine animals - fresh - Retail - Belgium - food sample - meat - batch 300 Gram N_A Detection 1 0 Escherichia coli 0 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Denmark - food sample - meat batch 300 Gram N_A Detection 4 0 Escherichia coli 0 - Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - France - food sample - meat - batch 300 Gram N_A Detection 27 4 Escherichia coli 4 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Germany - food sample - meat batch 300 Gram N_A Detection 5 0 Escherichia coli 0 - Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Greece - food sample - meat - batch 300 Gram N_A Detection 43 3 Escherichia coli 3 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Ireland - food sample - meat - batch 300 Gram N_A Detection 3 0 Escherichia coli 0 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Italy - food sample - meat - batch 300 Gram N_A Detection 9 1 Escherichia coli 1 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s

Greece - 2019 17 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Meat from bovine animals - fresh - Retail - Netherlands - food sample - batch 300 Gram N_A Detection 10 1 Escherichia coli 1 2010-2013) meat - Monitoring - EFSA specifications - Official sampling - Objective (food/fee method of sampling d) microorganism s Meat from bovine animals - fresh - Retail - Poland - food sample - meat - batch 300 Gram N_A Detection 1 0 Escherichia coli 0 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Romania - food sample - meat batch 300 Gram N_A Detection 2 1 Escherichia coli 1 - Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from bovine animals - fresh - Retail - Spain - food sample - meat - batch 300 Gram N_A Detection 5 0 Escherichia coli 0 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - Belgium - food sample - meat - Monitoring batch 300 Gram N_A Detection 1 0 Escherichia coli 0 - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - Denmark - food sample - meat - Monitoring batch 300 Gram N_A Detection 2 0 Escherichia coli 0 - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - France - food sample - meat - Monitoring - batch 300 Gram N_A Detection 10 0 Escherichia coli 0 EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - Germany - food sample - meat - Monitoring batch 300 Gram N_A Detection 4 0 Escherichia coli 0 - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - Greece - food sample - meat - Monitoring - batch 300 Gram N_A Detection 100 12 Escherichia coli 12 EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - Netherlands - food sample - meat - batch 300 Gram N_A Detection 55 2 Escherichia coli 2 Monitoring - EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - fresh - Retail - Spain - food sample - meat - Monitoring - batch 300 Gram N_A Detection 1 0 Escherichia coli 0 EFSA specifications - Official sampling - Objective sampling (food/fee method of d) microorganism s Meat from pig - meat preparation - intended to be eaten cooked - batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 Processing plant - Greece - food sample - meat - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Meat from pig - meat preparation - intended to be eaten cooked - batch 10 Gram N_A Not Available 10 0 Escherichia coli 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - batch 10 Gram N_A Not Available 35 0 Escherichia coli 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) Meat from pig - minced meat - Cutting plant - Greece - food sample - batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 meat - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) Meat, mixed meat - minced meat - intended to be eaten cooked - chilled - batch 10 Gram N_A Not Available 10 0 Escherichia coli 0 Processing plant - European Union - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Meat, mixed meat - minced meat - intended to be eaten cooked - chilled - batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Other food of non-animal origin - Hospital or medical care facility - Greece batch 10 Gram N_A Not Available 10 0 Escherichia coli 0 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) Greece - 2019 18 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Other food of non-animal origin - Processing plant - European Union - food single 10 Gram N_A Not Available 10 0 Escherichia coli 0 2010-2013) sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) Other food of non-animal origin - Processing plant - Greece - food sample single 10 Gram N_A Not Available 10 0 Escherichia coli 0 - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d) Other processed food products and prepared dishes - meat based dishes - batch 10 Gram N_A Not Available 50 0 Escherichia coli 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - potato based dishes batch 10 Gram N_A Not Available 15 0 Escherichia coli 0 - Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - rice based dishes - batch 10 Gram N_A Not Available 10 0 Escherichia coli 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - sandwiches - batch 10 Gram N_A Not Available 30 0 Escherichia coli 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - sandwiches - batch 10 Gram N_A Not Available 5 0 Escherichia coli 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - unspecified - ready- batch 10 Gram N_A Not Available 15 0 Escherichia coli 0 to-eat foods - Processing plant - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) Sweets - Hospital or medical care facility - Greece - food sample - batch 10 Gram N_A Not Available 15 0 Escherichia coli 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d)

Greece - 2019 19 Table Escherichia coli:ESCHERICHIA COLI in feed

Greece - 2019 20 Table Escherichia coli, non-pathogenic:ESCHERICHIA COLI, NON-PATHOGENIC in animal

Greece - 2019 21 Table FLAVIVIRUS in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling Vaccination units units Metrics N of units Area of Sampling strategy unit status Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 10 0 West Nile virus 0 2010-2013) sampling Available Birds - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - Selective animal Not N_A PCR 3 1 West Nile virus 1 sampling Available Gallus gallus (fowl) - laying hens - Farm - Greece - animal sample - Monitoring - active - Official sampling - animal Not N_A PCR 2 0 West Nile virus 0 Selective sampling Available Gallus gallus (fowl) - laying hens - Farm - Greece - animal sample - Monitoring - passive - Official sampling animal Not N_A PCR 7 0 West Nile virus 0 - Selective sampling Available Pigeons - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - animal Not N_A PCR 7 0 West Nile virus 0 Selective sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 425 23 West Nile virus Selective sampling ELISA (MAC- 23 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 808 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A Real-Time 31 0 West Nile virus Suspect sampling Reverse- 0 transcription PCR Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - Official sampling - Suspect animal No N_A IgM-capture 5 1 West Nile virus sampling ELISA (MAC- 1 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - Official sampling - Suspect animal No N_A ELISA, 8 0 West Nile virus sampling Competitive 0 ELISA (C- ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Unspecified - Official sampling - Not specified animal No N_A IgM-capture 5 0 West Nile virus ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Unspecified - Official sampling - Not specified animal No N_A ELISA, 3 0 West Nile virus Competitive 0 ELISA (C- ELISA) Attiki (NUTS Pigeons - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - animal Not N_A PCR 1 0 West Nile virus 0 2010-2013 level Selective sampling Available 2) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 53 3 West Nile virus Selective sampling ELISA (MAC- 3 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 70 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Λέσβος (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 4 0 West Nile virus 2010-2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 26 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Κυκλάδες (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 6 0 West Nile virus 2010-2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 9 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Λασίθι (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 6 0 West Nile virus 2010-2013) Selective sampling ELISA (MAC- 0 ELISA) Greece - 2019 22 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling Vaccination units units Metrics N of units Area of Sampling strategy unit status Sampling Details Method tested positive Zoonoses positive Λασίθι (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 69 0 West Nile virus 2010-2013) Selective sampling Competitive 0 ELISA (C- ELISA) Χανιά (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 3 0 West Nile virus 2010-2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 29 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Έβρος (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 4 0 West Nile virus 0 2013) sampling Available Gallus gallus (fowl) - laying hens - Farm - Greece - animal sample - Monitoring - active - Official sampling - animal Not N_A PCR 2 0 West Nile virus 0 Selective sampling Available Gallus gallus (fowl) - laying hens - Farm - Greece - animal sample - Monitoring - passive - Official sampling animal Not N_A PCR 7 0 West Nile virus 0 - Selective sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 5 0 West Nile virus Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 4 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Ξάνθη (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - Selective animal Not N_A PCR 1 1 West Nile virus 1 2013) sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 46 6 West Nile virus Selective sampling ELISA (MAC- 6 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 44 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Unspecified - Official sampling - Not specified animal Not N_A IgM-capture 4 0 West Nile virus Available ELISA (MAC- 0 ELISA) Δράμα (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 1 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 74 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Ημαθία (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 11 1 West Nile virus 2013) Selective sampling ELISA (MAC- 1 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 9 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Θεσσαλονίκη Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 2 0 West Nile virus 0 (NUTS 2013) sampling Available Pigeons - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - animal Not N_A PCR 6 0 West Nile virus 0 Selective sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 70 2 West Nile virus Selective sampling ELISA (MAC- 2 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 42 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Κιλκίς (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 8 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA)

Greece - 2019 23 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling Vaccination units units Metrics N of units Area of Sampling strategy unit status Sampling Details Method tested positive Zoonoses positive Κιλκίς (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 9 0 West Nile virus 2013) Selective sampling Competitive 0 ELISA (C- ELISA) Πέλλα (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 9 0 West Nile virus 2013) Selective sampling Competitive 0 ELISA (C- ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A Real-Time 31 0 West Nile virus Suspect sampling Reverse- 0 transcription PCR Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - Official sampling - Suspect animal No N_A IgM-capture 1 0 West Nile virus sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - Official sampling - Suspect animal No N_A ELISA, 8 0 West Nile virus sampling Competitive 0 ELISA (C- ELISA) Πιερία (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 1 0 West Nile virus 0 2013) sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 9 0 West Nile virus Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 2 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Σέρρες (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 20 2 West Nile virus 2013) Selective sampling ELISA (MAC- 2 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 16 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - Official sampling - Suspect animal No N_A IgM-capture 1 1 West Nile virus sampling ELISA (MAC- 1 ELISA) Χαλκιδική (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 1 0 West Nile virus 0 2013) sampling Available Birds - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - Selective animal Not N_A PCR 1 0 West Nile virus 0 sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 7 0 West Nile virus Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 25 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Καστοριά (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 5 0 West Nile virus 2013) Selective sampling Competitive 0 ELISA (C- ELISA) Φλώρινα (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 5 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 70 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Άρτα, Πρέβεζα Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 45 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA)

Greece - 2019 24 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling Vaccination units units Metrics N of units Area of Sampling strategy unit status Sampling Details Method tested positive Zoonoses positive Θεσπρωτία Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 10 0 West Nile virus (NUTS 2013) Selective sampling ELISA (MAC- 0 ELISA) Ιωάννινα (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - passive - Official sampling - Selective animal Not N_A PCR 1 0 West Nile virus 0 2013) sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 2 0 West Nile virus Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 64 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Καρδίτσα, Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 16 2 West Nile virus Τρίκαλα Selective sampling ELISA (MAC- 2 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 16 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Λάρισα (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 49 6 West Nile virus 2013) Selective sampling ELISA (MAC- 6 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 46 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Κέρκυρα (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 2 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 8 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Λευκάδα (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 1 0 West Nile virus 0 2013) sampling Available Solipeds, domestic - Farm - Greece - animal sample - blood - Unspecified - Official sampling - Not specified animal No N_A ELISA, 1 0 West Nile virus Competitive 0 ELISA (C- ELISA) Ηλεία (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 12 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 4 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Βοιωτία (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Unspecified - Official sampling - Not specified animal No N_A IgM-capture 1 0 West Nile virus 2013) ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Unspecified - Official sampling - Not specified animal No N_A ELISA, 2 0 West Nile virus Competitive 0 ELISA (C- ELISA) Εύβοια (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 1 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 32 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Ευρυτανία (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 1 0 West Nile virus 2013) Selective sampling ELISA (MAC- 0 ELISA)

Greece - 2019 25 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling Vaccination units units Metrics N of units Area of Sampling strategy unit status Sampling Details Method tested positive Zoonoses positive Ευρυτανία (NUTS Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 8 0 West Nile virus 2013) Selective sampling Competitive 0 ELISA (C- ELISA) Αργολίδα, Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 72 1 West Nile virus Αρκαδία Selective sampling ELISA (MAC- 1 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 70 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - Official sampling - Suspect animal No N_A IgM-capture 3 0 West Nile virus sampling ELISA (MAC- 0 ELISA) Κορινθία (NUTS Birds - wild - Natural habitat - Greece - animal sample - Monitoring - active - Official sampling - Selective animal Not N_A PCR 1 0 West Nile virus 0 2013) sampling Available Λακωνία, Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A IgM-capture 6 0 West Nile virus Μεσσηνία Selective sampling ELISA (MAC- 0 ELISA) Solipeds, domestic - Farm - Greece - animal sample - blood - Monitoring - active - Official sampling - animal No N_A ELISA, 4 0 West Nile virus Selective sampling Competitive 0 ELISA (C- ELISA)

Greece - 2019 26 Table Klebsiella:KLEBSIELLA in animal

Greece - 2019 27 Table Leishmania:LEISHMANIA in animal

Greece - 2019 28 Table Listeria:LISTERIA in animal

Greece - 2019 29 Table Listeria:LISTERIA in food

Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive GREECE(NUTS Cheeses made from cows' milk - soft and semi-soft - Hospital or medical care facility - Greece single 25 Gram N_A 1 0 detection Listeria monocytogenes 2010-2013) - food sample - Surveillance - based on Regulation 2073 - HACCP and own check - Selective (food/fee 1 0 sampling d) Cheeses made from goats' milk - soft and semi-soft - Processing plant - Greece - food sample single 25 Gram N_A 10 0 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Cheeses made from goats' milk - unspecified - Border Control Posts - Serbia - food sample - batch 25 Gram N_A 5 0 detection Listeria monocytogenes Monitoring - Official sampling - Objective sampling (food/fee 5 0 d) Cheeses made from sheep's milk - hard - made from pasteurised milk - Processing plant - batch 25 Gram N_A 5 0 detection Listeria monocytogenes Greece - food sample - Surveillance - based on Regulation 2073 - Official, based on (food/fee 5 0 Regulation 854/2004 - Objective sampling d) single 25 Gram N_A 5 0 detection Listeria monocytogenes (food/fee 5 0 d) Cheeses made from sheep's milk - soft and semi-soft - Processing plant - Greece - food batch 25 Gram N_A 10 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official, based on Regulation 854/2004 - (food/fee 10 0 Objective sampling d) single 25 Gram N_A 25 0 detection Listeria monocytogenes (food/fee 25 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - hard - made from single 25 Gram N_A 25 0 detection Listeria monocytogenes pasteurised milk - Processing plant - Greece - food sample - Surveillance - based on (food/fee 25 0 Regulation 2073 - Official, based on Regulation 854/2004 - Objective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - soft and semi-soft - single 25 Gram N_A 5 0 detection Listeria monocytogenes Processing plant - Greece - food sample - Surveillance - based on Regulation 2073 - Official, (food/fee 5 0 based on Regulation 854/2004 - Objective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - unspecified - Hospital or single 25 Gram N_A 1 0 detection Listeria monocytogenes medical care facility - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee 1 0 HACCP and own check - Selective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - unspecified - Processing single 25 Gram N_A 5 0 detection Listeria monocytogenes plant - Greece - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 5 0 Objective sampling d) Cheeses, made from unspecified milk or other animal milk - hard - made from pasteurised batch 25 Gram N_A 5 0 detection Listeria monocytogenes milk - Processing plant - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee 5 0 Official, based on Regulation 854/2004 - Objective sampling d) Dairy products (excluding cheeses) - chocolate milk - Processing plant - Greece - food single 25 Gram N_A 5 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - dairy desserts - Hospital or medical care facility - Greece single 25 Gram N_A 1 0 detection Listeria monocytogenes - food sample - Surveillance - based on Regulation 2073 - HACCP and own check - Selective (food/fee 1 0 sampling d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Border Control batch 25 Gram N_A 5 0 detection Listeria monocytogenes Posts - Serbia - food sample - Monitoring - Official sampling - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 25 Gram N_A 10 0 detection Listeria monocytogenes plant - Greece - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 10 0 Objective sampling d) Dairy products (excluding cheeses) - milk powder and whey powder - Border Control Posts - single 25 Gram N_A 25 0 detection Listeria monocytogenes Greece - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 25 0 Selective sampling d) Dairy products (excluding cheeses) - milk powder and whey powder - Processing plant - single 25 Gram N_A 2 0 detection Listeria monocytogenes Greece - food sample - Unspecified - Official sampling - Selective sampling (food/fee 2 0 d) Dairy products (excluding cheeses) - yoghurt - Hospital or medical care facility - Greece - food single 25 Gram N_A 1 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - HACCP and own check - Selective (food/fee 1 0 sampling d) Fish - cooked - Hospital or medical care facility - Greece - food sample - Surveillance - based single 25 Gram N_A 2 0 detection Listeria monocytogenes on Regulation 2073 - HACCP and own check - Selective sampling (food/fee 2 0 d)

Greece - 2019 30 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive GREECE(NUTS Fish - raw - frozen - Hospital or medical care facility - Greece - food sample - Surveillance - single 25 Gram N_A 1 0 detection Listeria monocytogenes 2010-2013) based on Regulation 2073 - HACCP and own check - Selective sampling (food/fee 1 0 d) Fish - smoked - Processing plant - Greece - food sample - Surveillance - based on Regulation single 25 Gram N_A 15 1 detection Listeria monocytogenes 2073 - Official, based on Regulation 854/2004 - Objective sampling (food/fee 15 1 d) Fishery products, unspecified - raw - frozen - Hospital or medical care facility - Greece - food single 25 Gram N_A 1 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - HACCP and own check - Selective (food/fee 1 0 sampling d) Foodstuffs intended for special nutritional uses - ready-to-eat - Retail - Greece - food sample - single 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Selective sampling (food/fee 5 0 d) Fruits and vegetables - pre-cut - Hospital or medical care facility - Greece - food sample - batch 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 5 0 d) Meat from bovine animals - fresh - frozen - Hospital or medical care facility - Greece - food single 25 Gram N_A 1 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - HACCP and own check - Selective (food/fee 1 0 sampling d) Meat from bovine animals - meat products - cooked, ready-to-eat - Hospital or medical care single 25 Gram N_A 1 0 detection Listeria monocytogenes facility - Greece - food sample - Surveillance - based on Regulation 2073 - HACCP and own (food/fee 1 0 check - Selective sampling d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Greece - food batch 25 Gram N_A 40 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 40 0 d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Greece - food single 25 Gram N_A 5 0 detection Listeria monocytogenes sample - Unspecified - Official, based on Regulation 854/2004 - Objective sampling (food/fee 5 0 d) Meat from pig - meat products - cooked, ready-to-eat - Retail - Greece - food sample - meat - batch 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official, based on Regulation 854/2004 - Objective (food/fee 5 0 sampling d) Meat from pig - meat products - fermented sausages - Processing plant - Greece - food single 25 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 sample - Surveillance - based on Regulation 2073 - Official, based on Regulation 854/2004 - (food/fee Objective sampling d) >100 Listeria monocytogenes 10 0 Milk, cows' - pasteurised milk - Processing plant - Greece - food sample - milk - Unspecified - single 25 Millilitre N_A 5 0 detection Listeria monocytogenes Official, based on Regulation 854/2004 - Selective sampling (food/fee 5 0 d) Other food of non-animal origin - Hospital or medical care facility - Greece - food sample - batch 25 Gram N_A 10 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Other food of non-animal origin - Processing plant - European Union - food sample - single 25 Gram N_A 10 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Other food of non-animal origin - Processing plant - Greece - food sample - Surveillance - single 25 Gram N_A 10 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Other processed food products and prepared dishes - fish and seafood based dishes - single 25 Gram N_A 1 0 detection Listeria monocytogenes Hospital or medical care facility - Greece - food sample - Surveillance - based on Regulation (food/fee 1 0 2073 - HACCP and own check - Selective sampling d) Other processed food products and prepared dishes - ices and similar frozen desserts - single 25 Gram N_A 5 0 detection Listeria monocytogenes Border Control Posts - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee 5 0 Official sampling - Selective sampling d) Other processed food products and prepared dishes - meat based dishes - Hospital or batch 25 Gram N_A 50 0 detection Listeria monocytogenes medical care facility - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee 50 0 Official sampling - Objective sampling d) Other processed food products and prepared dishes - potato based dishes - Hospital or batch 25 Gram N_A 15 0 detection Listeria monocytogenes medical care facility - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee 15 0 Official sampling - Objective sampling d) Other processed food products and prepared dishes - rice based dishes - Hospital or medical batch 25 Gram N_A 10 0 detection Listeria monocytogenes care facility - Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 10 0 sampling - Objective sampling d) Other processed food products and prepared dishes - sandwiches - Hospital or medical care batch 25 Gram N_A 30 0 detection Listeria monocytogenes facility - Greece - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 30 0 Objective sampling d)

Greece - 2019 31 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive GREECE(NUTS Other processed food products and prepared dishes - sandwiches - Processing plant - batch 25 Gram N_A 5 0 detection Listeria monocytogenes 2010-2013) Greece - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 5 0 Objective sampling d) Other processed food products and prepared dishes - unspecified - ready-to-eat foods - batch 25 Gram N_A 15 0 detection Listeria monocytogenes Processing plant - Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 15 0 sampling - Objective sampling d) Other processed food products and prepared dishes - vegetable based dishes - Hospital or single 25 Gram N_A 1 0 detection Listeria monocytogenes medical care facility - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee 1 0 HACCP and own check - Selective sampling d) Ready-to-eat salads - Hospital or medical care facility - Greece - food sample - Surveillance - single 25 Gram N_A 1 0 detection Listeria monocytogenes based on Regulation 2073 - HACCP and own check - Selective sampling (food/fee 1 0 d) Surimi - frozen - Border Control Posts - Greece - food sample - Surveillance - based on single 25 Gram N_A 5 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Selective sampling (food/fee 5 0 d) Sweets - Border Control Posts - Turkey - food sample - Monitoring - Official sampling - batch 25 Gram N_A 5 0 detection Listeria monocytogenes Objective sampling (food/fee 5 0 d) Sweets - Hospital or medical care facility - Greece - food sample - Surveillance - based on batch 25 Gram N_A 15 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 15 0 d)

Greece - 2019 32 Table Lyssavirus:LYSSAVIRUS in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive GREECE(NUTS Badgers - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 Lyssavirus 0 2010-2013) Selective sampling Bats - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Cats - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 4 0 Lyssavirus 0 - Selective sampling Cattle (bovine animals) - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 25 0 Lyssavirus 0 - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 507 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 728 0 Lyssavirus 0 sampling - Selective sampling Goats - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - Selective N_A Not Available animal 1 0 Lyssavirus 0 sampling Hedgehogs - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 Lyssavirus 0 Selective sampling Jackals - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Marten - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 Lyssavirus 0 Selective sampling Other mustelides - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 2 0 Lyssavirus 0 sampling - Selective sampling Rats - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 14 0 Lyssavirus 0 Selective sampling Solipeds, domestic - horses - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Wild boars - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Wolves - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 5 0 Lyssavirus 0 Selective sampling Attiki (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 2 0 Lyssavirus 0 2010-2013 level sampling - Selective sampling 2) Rats - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 12 0 Lyssavirus 0 Selective sampling Ηράκλειο (NUTS Hedgehogs - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 Lyssavirus 0 2010-2013) Selective sampling Έβρος (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 26 0 Lyssavirus 0 2013) sampling - Selective sampling Jackals - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Wolves - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Ξάνθη (NUTS Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 2013) - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 7 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 4 0 Lyssavirus 0 sampling - Selective sampling Marten - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Ροδόπη (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 38 0 Lyssavirus 0 2013) sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 23 0 Lyssavirus 0 sampling - Selective sampling Δράμα (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 26 0 Lyssavirus 0 2013) sampling - Selective sampling Greece - 2019 33 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Δράμα (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 62 0 Lyssavirus 0 2013) sampling - Selective sampling Wolves - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Θάσος, Καβάλα Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 3 0 Lyssavirus 0 sampling - Selective sampling Ημαθία (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 2013) sampling - Selective sampling Θεσσαλονίκη Cats - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 (NUTS 2013) - Selective sampling Cattle (bovine animals) - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 28 0 Lyssavirus 0 sampling - Selective sampling Rats - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Κιλκίς (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 17 0 Lyssavirus 0 2013) sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 22 0 Lyssavirus 0 sampling - Selective sampling Wolves - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Πέλλα (NUTS Badgers - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 Lyssavirus 0 2013) Selective sampling Cats - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 - Selective sampling Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 3 0 Lyssavirus 0 - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 20 0 Lyssavirus 0 sampling - Selective sampling Marten - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Rats - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Solipeds, domestic - horses - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 sampling - Selective sampling Πιερία (NUTS Cats - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 2013) - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 27 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 48 0 Lyssavirus 0 sampling - Selective sampling Σέρρες (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 145 0 Lyssavirus 0 2013) sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 220 0 Lyssavirus 0 sampling - Selective sampling Χαλκιδική (NUTS Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 2013) - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 10 0 Lyssavirus 0 sampling - Selective sampling Γρεβενά, Κοζάνη Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 53 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 148 0 Lyssavirus 0 sampling - Selective sampling Greece - 2019 34 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Καστοριά (NUTS Bats - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 2013) Selective sampling Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 7 0 Lyssavirus 0 - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 19 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 27 0 Lyssavirus 0 sampling - Selective sampling Other mustelides - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 2 0 Lyssavirus 0 sampling - Selective sampling Wild boars - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Φλώρινα (NUTS Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 2 0 Lyssavirus 0 2013) - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 9 0 Lyssavirus 0 sampling - Selective sampling Wolves - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Άρτα, Πρέβεζα Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 2 0 Lyssavirus 0 sampling - Selective sampling Θεσπρωτία Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 (NUTS 2013) - Selective sampling Ιωάννινα (NUTS Cats - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 2013) - Selective sampling Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 7 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 7 0 Lyssavirus 0 sampling - Selective sampling Wolves - wild - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 Lyssavirus 0 Selective sampling Καρδίτσα, Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 155 0 Lyssavirus 0 Τρίκαλα sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 58 0 Lyssavirus 0 sampling - Selective sampling Λάρισα (NUTS Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 2013) - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - active - Official N_A Not Available animal 10 0 Lyssavirus 0 sampling - Selective sampling Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 4 0 Lyssavirus 0 sampling - Selective sampling Μαγνησία, Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 Σποράδες - Selective sampling Λευκάδα (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 2 0 Lyssavirus 0 2013) sampling - Selective sampling Ηλεία (NUTS Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 3 0 Lyssavirus 0 2013) - Selective sampling Φθιώτιδα (NUTS Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 2 0 Lyssavirus 0 2013) - Selective sampling Φωκίδα (NUTS Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 2013) sampling - Selective sampling Αργολίδα, Dogs - pet animals - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling N_A Not Available animal 1 0 Lyssavirus 0 Αρκαδία - Selective sampling Goats - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official sampling - Selective N_A Not Available animal 1 0 Lyssavirus 0 sampling Λακωνία, Foxes - wild - red fox - Natural habitat - Greece - animal sample - brain - Monitoring - passive - Official N_A Not Available animal 1 0 Lyssavirus 0 Μεσσηνία sampling - Selective sampling

Greece - 2019 35 Table Proteus:PROTEUS in animal

Greece - 2019 36 Table Salmonella:SALMONELLA in animal

N of flocks Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling under control Target Total units units Metrics N of units Area of Sampling strategy unit programme verification Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Cattle (bovine animals) - calves (under 1 year) - Farm - Greece - animal sample - foetus/stillbirth - Clinical animal N_A N_A ISO 1 0 Salmonella 2010-2013) investigations - Official sampling - Suspect sampling 6579:2002/Am d 1:2007 0 (Annex D of ISO 6579) Salmonella Cattle (bovine animals) - calves (under 1 year) - Farm - Greece - animal sample - organ/tissue - Clinical animal N_A N_A ISO 1 0 Salmonella investigations - Official sampling - Suspect sampling 6579:2002/Am d 1:2007 0 (Annex D of ISO 6579) Salmonella Cattle (bovine animals) - Farm - Greece - animal sample - organ/tissue - Clinical investigations - Official animal N_A N_A Not Available 1 0 Salmonella spp., unspecified 0 sampling - Suspect sampling Gallus gallus (fowl) - breeding flocks, unspecified - adult - Farm - Greece - animal sample - Control and herd/floc 237 Y N_A Not Available 236 27 Salmonella Kottbus 1 eradication programmes - Official and industry sampling - Census k Salmonella Livingstone 23 Salmonella Paratyphi B 2 Salmonella Szentes 2 Salmonella Typhimurium 1 Gallus gallus (fowl) - breeding flocks, unspecified - day-old chicks - Farm - Greece - animal sample - Control herd/floc 114 N_A N_A Not Available 113 0 Salmonella 0 and eradication programmes - Industry sampling - Census k Gallus gallus (fowl) - breeding flocks, unspecified - during rearing period - Farm - Greece - animal sample - herd/floc 140 N_A N_A Not Available 139 0 Salmonella 0 Control and eradication programmes - Industry sampling - Census k Gallus gallus (fowl) - broilers - before slaughter - Farm - Greece - animal sample - Control and eradication herd/floc 8893 N_A N_A Not Available 8059 0 Salmonella 0 programmes - Industry sampling - Census k Gallus gallus (fowl) - broilers - before slaughter - Farm - Greece - animal sample - Control and eradication herd/floc 8893 Y N_A Not Available 8061 17 Salmonella Cerro 1 programmes - Official and industry sampling - Census k Salmonella Enterica, 1 unspecified Salmonella Hadar 1 Salmonella Infantis 1 Salmonella Kedougou 1 Salmonella Livingstone 4 Salmonella Muenchen 1 Salmonella Muenster 1 Salmonella Senftenberg 5 Salmonella Umbilo 1 Gallus gallus (fowl) - broilers - before slaughter - Farm - Greece - animal sample - Control and eradication herd/floc 8893 N_A N_A Not Available 88 17 Salmonella Cerro 1 programmes - Official sampling - Objective sampling k Salmonella Enterica, 1 unspecified Salmonella Hadar 1 Salmonella Infantis 1 Salmonella Kedougou 1 Salmonella Livingstone 4 Salmonella Muenchen 1 Salmonella Muenster 1 Salmonella Senftenberg 5 Salmonella Umbilo 1 Gallus gallus (fowl) - laying hens - adult - Farm - Greece - animal sample - Control and eradication herd/floc 857 Y N_A Not Available 681 47 Salmonella Bareilly 1 programmes - Official and industry sampling - Census k Salmonella Bloomsbury 1 Salmonella Bovismorbificans 3 Salmonella Braenderup 5 Salmonella Bredeney 1 Salmonella Edinburg 1 Salmonella Enterica, 2 unspecified Greece - 2019 37 N of flocks Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling under control Target Total units units Metrics N of units Area of Sampling strategy unit programme verification Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Gallus gallus (fowl) - laying hens - adult - Farm - Greece - animal sample - Control and eradication herd/floc 857 Y N_A Not Available 681 47 Salmonella Enteritidis 4 2010-2013) programmes - Official and industry sampling - Census k Salmonella Havana 1 Salmonella Herston 1 Salmonella Indiana 4 Salmonella Infantis 7 Salmonella Kentucky 1 Salmonella Kottbus 3 Salmonella Lexington 1 Salmonella Mishmarhaemek 1 Salmonella Montevideo 1 Salmonella Muenchen 1 Salmonella Oranienburg 1 Salmonella Other serovars 1 Salmonella Senftenberg 1 Salmonella Szentes 1 Salmonella Thompson 2 Salmonella Typhimurium 1 Salmonella Uno 1 Gallus gallus (fowl) - laying hens - day-old chicks - Farm - Greece - animal sample - Control and eradication herd/floc 92 N_A N_A Not Available 83 0 Salmonella 0 programmes - Industry sampling - Census k Gallus gallus (fowl) - laying hens - during rearing period - Farm - Greece - animal sample - Control and herd/floc 131 N_A N_A Not Available 120 0 Salmonella 0 eradication programmes - Industry sampling - Census k Goats - animals over 1 year - Farm - Greece - animal sample - organ/tissue - Clinical investigations - Official animal N_A N_A Not Available 6 1 Salmonella 1 sampling - Suspect sampling Goats - animals under 1 year - Farm - Greece - animal sample - foetus/stillbirth - Clinical investigations - animal N_A N_A Not Available 5 0 Salmonella 0 Official sampling - Suspect sampling Goats - animals under 1 year - Farm - Greece - animal sample - organ/tissue - Clinical investigations - animal N_A N_A Not Available 6 2 Salmonella 2 Official sampling - Suspect sampling Mice - laboratory animal - Unspecified - Greece - animal sample - organ/tissue - Unspecified - Official animal N_A N_A Not Available 10 0 Salmonella 0 sampling - Objective sampling Sheep - animals over 1 year - Farm - Greece - animal sample - organ/tissue - Clinical investigations - Official animal N_A N_A Not Available 9 1 Salmonella 1 sampling - Suspect sampling Sheep - animals under 1 year (lambs) - Farm - Greece - animal sample - foetus/stillbirth - Clinical animal N_A N_A Not Available 7 0 Salmonella 0 investigations - Official sampling - Suspect sampling Sheep - animals under 1 year (lambs) - Farm - Greece - animal sample - organ/tissue - Clinical animal N_A N_A Not Available 2 2 Salmonella 2 investigations - Official sampling - Suspect sampling Turkeys - breeding flocks, unspecified - adult - Farm - Greece - animal sample - Control and eradication herd/floc 10 N_A N_A Not Available 8 0 Salmonella 0 programmes - Industry sampling - Census k Turkeys - breeding flocks, unspecified - adult - Farm - Greece - animal sample - Control and eradication herd/floc 10 Y N_A Not Available 10 0 Salmonella 0 programmes - Official and industry sampling - Census k Turkeys - breeding flocks, unspecified - adult - Farm - Greece - animal sample - Control and eradication herd/floc 10 N_A N_A Not Available 2 0 Salmonella 0 programmes - Official sampling - Objective sampling k Turkeys - fattening flocks - before slaughter - Farm - Greece - animal sample - Control and eradication herd/floc 86 N_A N_A Not Available 60 0 Salmonella 0 programmes - Industry sampling - Census k Turkeys - fattening flocks - before slaughter - Farm - Greece - animal sample - Control and eradication herd/floc 86 Y N_A Not Available 67 0 Salmonella 0 programmes - Official and industry sampling - Census k Turkeys - fattening flocks - before slaughter - Farm - Greece - animal sample - Control and eradication herd/floc 86 N_A N_A Not Available 7 0 Salmonella 0 programmes - Official sampling - Objective sampling k

Greece - 2019 38 Table Salmonella:SALMONELLA in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Bakery products - desserts - containing raw eggs - Hospital or medical single 25 Gram N_A Not Available 2 0 Salmonella 0 2010-2013) care facility - Greece - food sample - Surveillance - based on Regulation (food/fee 2073 - HACCP and own check - Selective sampling d) Bakery products - desserts - containing raw eggs - Retail - Greece - food single 25 Gram N_A Not Available 1 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Selective sampling d) Cheeses made from cows' milk - soft and semi-soft - Hospital or medical single 25 Gram N_A Not Available 1 0 Salmonella 0 care facility - Greece - food sample - Surveillance - based on Regulation (food/fee 2073 - HACCP and own check - Selective sampling d) Cheeses made from sheep's milk - hard - made from pasteurised milk - batch 25 Gram N_A Not Available 5 0 Salmonella spp., unspecified 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official, based on Regulation 854/2004 - Objective d) sampling Cheeses made from sheep's milk - soft and semi-soft - made from batch 25 Gram N_A Not Available 10 0 Salmonella spp., unspecified 0 pasteurised milk - Processing plant - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official, based on Regulation 854/2004 - d) Objective sampling Cheeses, made from mixed milk from cows, sheep and/or goats - Hospital single 25 Gram N_A Not Available 1 0 Salmonella 0 or medical care facility - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - HACCP and own check - Selective sampling d) Cheeses, made from unspecified milk or other animal milk - hard - made batch 25 Gram N_A Not Available 5 0 Salmonella spp., unspecified 0 from pasteurised milk - Processing plant - Greece - food sample - (food/fee Surveillance - based on Regulation 2073 - Official, based on Regulation d) 854/2004 - Objective sampling Crustaceans - unspecified - cooked - chilled - Retail - Greece - food single 25 Gram N_A Not Available 1 0 Salmonella 0 sample - Unspecified - Official sampling - Suspect sampling (food/fee d) Dairy products (excluding cheeses) - chocolate milk - Processing plant - single 25 Gram N_A Not Available 5 0 Salmonella 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) Dairy products (excluding cheeses) - dairy desserts - Hospital or medical single 25 Gram N_A Not Available 2 0 Salmonella 0 care facility - Greece - food sample - Surveillance - based on Regulation (food/fee 2073 - HACCP and own check - Selective sampling d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised single 25 Gram N_A Not Available 10 0 Salmonella 0 milk - Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Dairy products (excluding cheeses) - milk powder and whey powder - single 25 Gram N_A Not Available 20 0 Salmonella 0 Border Control Posts - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Selective sampling d) Dairy products (excluding cheeses) - milk powder and whey powder - single 25 Gram N_A Not Available 2 0 Salmonella 0 Processing plant - Greece - food sample - Unspecified - Official sampling - (food/fee Selective sampling d) Dairy products (excluding cheeses) - yoghurt - Hospital or medical care single 25 Gram N_A Not Available 1 0 Salmonella 0 facility - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee HACCP and own check - Selective sampling d) Eggs - table eggs - Hospital or medical care facility - Greece - food sample single 25 Gram N_A Not Available 1 0 Salmonella 0 - Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee Selective sampling d) Fish - cooked - frozen - Retail - Greece - food sample - Unspecified - single 25 Gram N_A Not Available 1 0 Salmonella 0 Official sampling - Suspect sampling (food/fee d) Fish - cooked - Hospital or medical care facility - Greece - food sample - single 25 Gram N_A Not Available 2 0 Salmonella 0 Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee Selective sampling d) Fish - raw - frozen - Border Control Posts - Greece - food sample - single 25 Gram N_A Not Available 4 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Selective (food/fee sampling d)

Greece - 2019 39 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Fish - raw - frozen - Hospital or medical care facility - Greece - food single 25 Gram N_A Not Available 3 0 Salmonella 0 2010-2013) sample - Surveillance - based on Regulation 2073 - HACCP and own check (food/fee - Selective sampling d) Fishery products, unspecified - Hospital or medical care facility - Greece - single 25 Gram N_A Not Available 2 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - HACCP and own (food/fee check - Selective sampling d) Fruits and vegetables - pre-cut - Hospital or medical care facility - Greece batch 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Live bivalve molluscs - Border Control Posts - Greece - food sample - single 25 Gram N_A Not Available 5 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Selective (food/fee sampling d) Live bivalve molluscs - Farm - Greece - food sample - Surveillance - based batch 25 Gram N_A Not Available 2 0 Salmonella 0 on Regulation 2073 - Official sampling - Objective sampling (food/fee d) Live bivalve molluscs - Farm - Greece - food sample - Surveillance - based single 25 Gram N_A Not Available 3 0 Salmonella 0 on Regulation 2073 - Official sampling - Selective sampling (food/fee d) Live bivalve molluscs - Farm - Greece - food sample - Surveillance - batch 25 Gram N_A ISO 6579:2002 15 1 Salmonella Saphra 1 Official, based on Regulation 854/2004 - Objective sampling (food/fee Salmonella d) Live bivalve molluscs - mussels - Border Control Posts - Greece - food single 25 Gram N_A Not Available 5 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Selective sampling d) Live bivalve molluscs - mussels - Farm - Greece - food sample - batch 25 Gram N_A Not Available 2 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d) Meat from bovine animals - carcase - Slaughterhouse - Greece - food slaughte 400 Square N_A Not Available 20 1 Salmonella Kingston 1 sample - carcase swabs - Surveillance - based on Regulation 2073 - r animal centimetre Official, based on Regulation 854/2004 - Objective sampling batch Meat from bovine animals - fresh - frozen - Hospital or medical care single 25 Gram N_A Not Available 3 0 Salmonella 0 facility - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee HACCP and own check - Selective sampling d) Meat from bovine animals - meat products - cooked, ready-to-eat - single 25 Gram N_A Not Available 1 0 Salmonella 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - HACCP and own check - Selective sampling d) Meat from bovine animals - minced meat - Processing plant - Greece - batch 25 Gram N_A Not Available 5 0 Salmonella 0 food sample - meat - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) Meat from broilers (Gallus gallus) - carcase - chilled - Slaughterhouse - single 25 Gram N_A Not Available 20 0 Salmonella 0 Greece - food sample - neck skin - Surveillance - based on Regulation (food/fee 2073 - Official, based on Regulation 854/2004 - Objective sampling d) Meat from broilers (Gallus gallus) - carcase - Slaughterhouse - Greece - slaughte 25 Gram N_A Not Available 40 18 Salmonella Infantis 13 food sample - neck skin - Surveillance - based on Regulation 2073 - r animal Salmonella Virchow 5 Official sampling - Objective sampling batch Meat from broilers (Gallus gallus) - carcase - Slaughterhouse - Not single 25 Gram N_A ISO 6579- 45 0 Salmonella 0 Available - food sample - neck skin - Surveillance - based on Regulation (food/fee 1:2017 2073 - HACCP and own check - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - fresh - chilled - Retail - Greece - food single 25 Gram N_A Not Available 30 0 Salmonella 0 sample - meat - Surveillance - based on Regulation 2073 - Official, based (food/fee on Regulation 854/2004 - Objective sampling d) Meat from broilers (Gallus gallus) - fresh - Processing plant - Greece - food batch 25 Gram N_A ISO 6579- 10 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - fresh - skinned - Cutting plant - Greece batch 25 Gram N_A Not Available 5 0 Salmonella 0 - food sample - meat - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) Meat from broilers (Gallus gallus) - meat preparation - intended to be batch 25 Gram N_A Not Available 5 0 Salmonella 0 eaten cooked - chilled - Cutting plant - Greece - food sample - meat - (food/fee Surveillance - based on Regulation 2073 - Official sampling - Objective d) sampling Greece - 2019 40 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Meat from broilers (Gallus gallus) - meat preparation - intended to be batch 25 Gram N_A Not Available 5 0 Salmonella 0 2010-2013) eaten cooked - Cutting plant - Greece - food sample - meat - Surveillance (food/fee - based on Regulation 2073 - Official sampling - Objective sampling d) Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A Not Available 20 7 Salmonella Livingstone 7 eaten cooked - Retail - Greece - food sample - meat - Surveillance - based (food/fee on Regulation 2073 - Official, based on Regulation 854/2004 - Objective d) sampling Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A Not Available 20 4 Salmonella Infantis 4 eaten cooked - Slaughterhouse - Greece - food sample - neck skin - (food/fee Surveillance - based on Regulation 2073 - Official, based on Regulation d) 854/2004 - Objective sampling Meat from broilers (Gallus gallus) - meat products - raw but intended to single 25 Gram N_A Not Available 5 2 Salmonella 2 be eaten cooked - chilled - Retail - Greece - food sample - Unspecified - (food/fee Official sampling - Suspect sampling d) Meat from broilers (Gallus gallus) - meat products - raw but intended to batch 25 Gram N_A ISO 6579- 25 0 Salmonella 0 be eaten cooked - Processing plant - Greece - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from goat - carcase - Slaughterhouse - Greece - food sample - single 400 Square N_A Not Available 5 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - Official (food/fee centimetre sampling - Selective sampling d) Meat from pig - carcase - Slaughterhouse - Greece - food sample - carcase slaughte 400 Square N_A Not Available 5 0 Salmonella 0 swabs - Surveillance - based on Regulation 2073 - Official, based on r animal centimetre Regulation 854/2004 - Objective sampling batch Meat from pig - carcase - Slaughterhouse - Not Available - food sample - slaughte 400 Square N_A Not Available 651 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - HACCP and r animal centimetre own check - Objective sampling batch Meat from pig - carcase - Slaughterhouse - Not Available - food sample - slaughte 400 Square N_A ISO 6579- 125 1 Salmonella Adabraka 1 carcase swabs - Surveillance - based on Regulation 2073 - Official, based r animal centimetre 1:2017 on Regulation 854/2004 - Objective sampling batch Salmonella Meat from pig - fresh - chilled - Hospital or medical care facility - Greece - single 25 Gram N_A Not Available 1 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - HACCP and own (food/fee check - Selective sampling d) Meat from pig - meat preparation - intended to be eaten cooked - batch 10 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from pig - meat preparation - intended to be eaten cooked - Retail - single 10 Gram N_A Not Available 10 0 Salmonella 0 Greece - food sample - meat - Surveillance - based on Regulation 2073 - (food/fee Official, based on Regulation 854/2004 - Objective sampling d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - batch 25 Gram N_A ISO 6579- 35 0 Salmonella 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - meat products - cooked, ready-to-eat - Retail - Greece - single 10 Gram N_A Not Available 10 0 Salmonella 0 food sample - meat - Surveillance - based on Regulation 2073 - Official, (food/fee based on Regulation 854/2004 - Objective sampling d) Meat from pig - meat products - fermented sausages - Processing plant - single 25 Gram N_A Not Available 10 0 Salmonella 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official, (food/fee based on Regulation 854/2004 - Objective sampling d) Meat from pig - minced meat - Processing plant - Greece - food sample - batch 25 Gram N_A Not Available 5 0 Salmonella 0 meat - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) Meat from poultry, unspecified - carcase - chilled - Slaughterhouse - single 25 Gram N_A Not Available 5 4 Salmonella 4 Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Selective sampling d) Meat from poultry, unspecified - fresh - Hospital or medical care facility - single 25 Gram N_A Not Available 4 0 Salmonella 0 Greece - food sample - Surveillance - based on Regulation 2073 - HACCP (food/fee and own check - Selective sampling d) Meat from sheep - carcase - Slaughterhouse - Greece - food sample - slaughte 400 Square N_A Not Available 35 2 Salmonella enterica, 2 carcase swabs - Surveillance - based on Regulation 2073 - Official, based r animal centimetre subspecies diarizonae on Regulation 854/2004 - Objective sampling batch

Greece - 2019 41 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Meat, mixed meat - meat preparation - intended to be eaten cooked - single 10 Gram N_A Not Available 5 0 Salmonella 0 2010-2013) Retail - Greece - food sample - meat - Surveillance - based on Regulation (food/fee 2073 - Official, based on Regulation 854/2004 - Objective sampling d) Meat, mixed meat - minced meat - intended to be eaten cooked - chilled - batch 10 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - European Union - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat, mixed meat - minced meat - intended to be eaten cooked - chilled - batch 10 Gram N_A ISO 6579- 5 1 Salmonella Rissen 1 Processing plant - Greece - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat, mixed meat - minced meat - intended to be eaten cooked - batch 10 Gram N_A ISO 6579- 15 0 Salmonella 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella 25 Gram N_A ISO 6579- 15 0 Salmonella 0 1:2017 Salmonella Milk, cows' - pasteurised milk - Processing plant - Greece - food sample - single 25 Millilitre N_A Not Available 5 0 Salmonella 0 milk - Unspecified - Official, based on Regulation 854/2004 - Selective (food/fee sampling d) Other food - Processing plant - Greece - food sample - Surveillance - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Other food of non-animal origin - Hospital or medical care facility - Greece batch 25 Gram N_A ISO 6579- 10 0 Salmonella 0 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other food of non-animal origin - Processing plant - European Union - food single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Other food of non-animal origin - Processing plant - Greece - food sample single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Other processed food products and prepared dishes - fish and seafood single 25 Gram N_A Not Available 1 0 Salmonella 0 based dishes - Hospital or medical care facility - Greece - food sample - (food/fee Surveillance - based on Regulation 2073 - HACCP and own check - d) Selective sampling Other processed food products and prepared dishes - ices and similar single 25 Gram N_A Not Available 5 0 Salmonella 0 frozen desserts - Border Control Posts - Greece - food sample - (food/fee Surveillance - based on Regulation 2073 - Official sampling - Selective d) sampling Other processed food products and prepared dishes - meat based dishes - batch 25 Gram N_A ISO 6579- 50 0 Salmonella 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - potato based dishes batch 25 Gram N_A ISO 6579- 15 0 Salmonella 0 - Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - rice based dishes - batch 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - single 25 Gram N_A Not Available 1 0 Salmonella 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - HACCP and own check - Objective sampling d) Other processed food products and prepared dishes - sandwiches - batch 25 Gram N_A Not Available 30 0 Salmonella 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - sandwiches - batch 25 Gram N_A Not Available 5 0 Salmonella 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - unspecified - ready- batch 25 Gram N_A ISO 6579- 15 0 Salmonella 0 to-eat foods - Processing plant - Greece - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella

Greece - 2019 42 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Other processed food products and prepared dishes - vegetable based single 25 Gram N_A Not Available 1 0 Salmonella 0 2010-2013) dishes - Hospital or medical care facility - Greece - food sample - (food/fee Surveillance - based on Regulation 2073 - HACCP and own check - d) Selective sampling Other products of animal origin - gelatin and collagen - Border Control single 25 Gram N_A Not Available 10 0 Salmonella 0 Posts - Greece - food sample - Surveillance - based on Regulation 2073 - (food/fee Official sampling - Selective sampling d) Ready-to-eat salads - Hospital or medical care facility - Greece - food single 25 Gram N_A Not Available 1 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - HACCP and own check (food/fee - Selective sampling d) Seeds, dried - flour/meal or finely ground powder - Hospital or medical single 25 Gram N_A Not Available 1 0 Salmonella 0 care facility - Greece - food sample - Surveillance - based on Regulation (food/fee 2073 - HACCP and own check - Selective sampling d) Surimi - frozen - Border Control Posts - Greece - food sample - single 25 Gram N_A Not Available 15 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Selective (food/fee sampling d) Sweets - Border Control Posts - Turkey - food sample - Monitoring - batch 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Sweets - Hospital or medical care facility - Greece - food sample - batch 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella

Greece - 2019 43 Table Salmonella:SALMONELLA in feed

Greece - 2019 44 Table Staphylococcal enterotoxins:STAPHYLOCOCCAL ENTEROTOXINS in food

Greece - 2019 45 Table Staphylococcus:STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) in animal

Greece - 2019 46 Table Staphylococcus:STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Cheeses made from cows' milk - soft and semi-soft - Hospital or medical single 10 Gram N_A Not Available 1 0 Staphylococcus 0 2010-2013) care facility - Greece - food sample - Surveillance - based on Regulation (food/fee 2073 - HACCP and own check - Selective sampling d) Cheeses made from sheep's milk - hard - made from pasteurised milk - batch 10 Gram N_A Not Available 5 0 Staphylococcus 0 Processing plant - Greece - food sample - Surveillance - based on (food/fee Regulation 2073 - Official, based on Regulation 854/2004 - Objective d) sampling Cheeses made from sheep's milk - soft and semi-soft - Processing plant - batch 10 Gram N_A Not Available 10 0 Staphylococcus 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official, (food/fee based on Regulation 854/2004 - Objective sampling d) Cheeses, made from unspecified milk or other animal milk - hard - made batch 10 Gram N_A Not Available 5 0 Staphylococcus 0 from pasteurised milk - Processing plant - Greece - food sample - (food/fee Surveillance - based on Regulation 2073 - Official, based on Regulation d) 854/2004 - Objective sampling Crustaceans - unspecified - cooked - chilled - Retail - Greece - food single 10 Gram N_A Not Available 1 0 Staphylococcus 0 sample - Unspecified - Official sampling - Suspect sampling (food/fee d) Dairy products (excluding cheeses) - milk powder and whey powder - single 10 Gram N_A Not Available 2 0 Staphylococcus 0 Processing plant - Greece - food sample - Unspecified - Official sampling - (food/fee Selective sampling d) Fish - cooked - frozen - Retail - Greece - food sample - Unspecified - single 10 Gram N_A Not Available 1 0 Staphylococcus 0 Official sampling - Suspect sampling (food/fee d) Foodstuffs intended for special nutritional uses - ready-to-eat - Retail - single 10 Gram N_A Not Available 5 0 Staphylococcus 0 Greece - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Selective sampling d) Fruits and vegetables - pre-cut - Hospital or medical care facility - Greece batch 10 Gram N_A EN ISO 6888- 5 0 Staphylococcus 0 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:1999/Amdt sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci Other food of non-animal origin - Hospital or medical care facility - Greece batch 10 Gram N_A EN ISO 6888- 10 0 Staphylococcus 0 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:1999/Amdt sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci Other processed food products and prepared dishes - meat based dishes - batch 10 Gram N_A EN ISO 6888- 50 0 Staphylococcus 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:1999/Amdt based on Regulation 2073 - Official sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci Other processed food products and prepared dishes - potato based dishes batch 10 Gram N_A EN ISO 6888- 15 0 Staphylococcus 0 - Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:1999/Amdt based on Regulation 2073 - Official sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci Other processed food products and prepared dishes - rice based dishes - batch 10 Gram N_A EN ISO 6888- 10 0 Staphylococcus 0 Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:1999/Amdt based on Regulation 2073 - Official sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci

Greece - 2019 47 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive GREECE(NUTS Other processed food products and prepared dishes - sandwiches - batch 10 Gram N_A EN ISO 6888- 30 0 Staphylococcus 0 2010-2013) Hospital or medical care facility - Greece - food sample - Surveillance - (food/fee 1:1999/Amdt based on Regulation 2073 - Official sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci Sweets - Hospital or medical care facility - Greece - food sample - batch 10 Gram N_A EN ISO 6888- 15 0 Staphylococcus 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:1999/Amdt sampling d) 1:2003 Coagulase- positives staphylococci

Greece - 2019 48 Table Streptococcus:STREPTOCOCCUS in animal

Greece - 2019 49 Table Toxoplasma:TOXOPLASMA in animal

Greece - 2019 50 Table Trichinella:TRICHINELLA in animal

Greece - 2019 51 FOODBORNE OUTBREAKS TABLES

Foodborne Outbreaks: summarized data when numbers referring to cases, hospitalized people and deaths are reported as unknown, they will be not included in the sum calculation

Outbreak strenght Strong Weak N N Metrics Causative agent Food vehicle N outbreaks N human cases hospitalized N deaths N outbreaks N human cases hospitalized N deaths Calicivirus Tap water, including well water 1 638 23 0 Clostridium perfringens Bovine meat and products thereof 1 58 10 0 Salmonella Enteritidis Broiler meat (Gallus gallus) and products thereof 1 13 3 0 Salmonella spp., unspecified Bovine meat and products thereof 1 11 3 0 Unknown Broiler meat (Gallus gallus) and products thereof 1 15 0 0 Tap water, including well water 1 72 0 0

Greece - 2019 52 Strong Foodborne Outbreaks: detailed data

M e tr i N Causative Other Causative FBO nat. More food vehicle Nature of Place of origin Origin of food Contributory c N human N N CAUSATIVEOUTBREAKCAUSATIVEREPORTINGagent AGENT STRENGTH AGENT YEAR GROUPH GROUPAG VT Agent code Outbreak type Food vehicle info evidence Setting of problem vehicle factors Comment s outbreaks cases hosp. deaths N YS N2Calicivirus unk Not Not VTEC N_A General Tap water, including N_A Analytical Household Water Not Unknown N_A o et o0 Availabl Availabl O157;Enteropath well water epidemiologic distribution Available t sr t 1 e e ogenic E. coli al evidence system 1 638 23 0 A o A9 (EPEC);Campylo v n v bacter, a g a unspecified sp. i i Clostridium unk Not Not Not Available N_A General Bovine meat and N_A Detection of Restaurant Restaurant or Not Storage N_A l l perfringens Availabl Availabl products thereof causative or Cafe or Cafe or Pub or Available time/tempera a a e e agent in food Pub or Bar Bar or Hotel or ture abuse b b vehicle or its or Hotel or Catering l l component - Catering service e e Symptoms service and onset of illness 1 58 10 0 pathognomon ic to causative agent;Analyti cal epidemiologic al evidence

Greece - 2019 53 Weak Foodborne Outbreaks: detailed data

M e tr i N Causative Other Causative FBO nat. More food vehicle Nature of Place of origin Origin of food Contributory c N human N N CAUSATIVECAUSATIVEOUTBREAKREPORTINGagent AGENT AGENT STRENGTH YEAR GROUP GROUPH AG VT Agent code Outbreak type Food vehicle info evidence Setting of problem vehicle factors Comment s outbreaks cases hosp. deaths NNNW2Salmonella un Not Not Not Available N_A General Broiler meat N_A Descriptiv Restaurant or Restaurant or Not Available Unknown N_A o ooe0Enteritidis k Available Available (Gallus gallus) and e Cafe or Pub Cafe or Pub t t a1 products thereof environme or Bar or or Bar or 1 13 3 0 AA k9 ntal Hotel or Hotel or v v evidence Catering Catering a a service service i i Salmonella un Not Not Not Available N_A General Bovine meat and N_A Descriptiv Others Others Not Available Storage This was an l l spp., k Available Available products thereof e time/temperatur outbreak a a unspecified environme e abuse which 1 11 3 0 b b ntal occured in a l l evidence military camp e e Unknown un Not Not Not Available N_A General Broiler meat N_A Descriptiv Restaurant or Restaurant or Not Available Unknown N_A k Available Available (Gallus gallus) and e Cafe or Pub Cafe or Pub products thereof environme or Bar or or Bar or 1 15 0 0 ntal Hotel or Hotel or evidence Catering Catering service service Tap water, N_A Descriptiv Household Water Not Available Unknown N_A including well e distribution water environme system 1 72 0 0 ntal evidence

Greece - 2019 54 ANTIMICROBIAL RESISTANCE TABLES FOR CAMPYLOBACTER

Greece - 2019 55 ANTIMICROBIAL RESISTANCE TABLES FOR SALMONELLA

Table Antimicrobial susceptibility testing of Salmonella Adabraka in Meat from pig - carcase

Sampling Stage: Slaughterhouse Sampling Type: food sample - carcase swabs Sampling Context: Surveillance - based on Regulation 2073 Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 32 1

Greece - 2019 56 Table Antimicrobial susceptibility testing of Salmonella Bareilly in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 128 1

Greece - 2019 57 Table Antimicrobial susceptibility testing of Salmonella Bovismorbificans in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 1

Greece - 2019 58 Table Antimicrobial susceptibility testing of Salmonella Braenderup in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 1 0 0 0 0 1 0 0 0 1 0 0 0 0 s N <=0.015 2 <=0.03 3 <=0.25 3 3 3 <=0.5 3 3 0.5 1 <=1 2 3 <=2 3 <=4 1 4 1 <=8 3 1 8 1 1 16 1 32 1 >64 1 128 2

Greece - 2019 59 Table Antimicrobial susceptibility testing of Salmonella Bredeney in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 1 <=4 1 <=8 1 128 1

Greece - 2019 60 Table Antimicrobial susceptibility testing of Salmonella Cerro in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 <=2 1 2 1 <=4 1 4 1 <=8 1 64 1

Greece - 2019 61 Table Antimicrobial susceptibility testing of Salmonella Enteritidis in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 5 <=0.03 5 <=0.25 5 5 4 <=0.5 5 5 <=1 5 4 1 1 <=2 2 5 2 1 <=4 5 4 3 <=8 5 3 32 1 256 1

Greece - 2019 62 Table Antimicrobial susceptibility testing of Salmonella group C1 in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 1 0 0 0 0 0 0 0 0 s N <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 0.5 1 <=1 1 1 <=2 1 <=8 1 1 8 1 16 1

Greece - 2019 63 Table Antimicrobial susceptibility testing of Salmonella Herston in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 1 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 1 <=4 1 <=8 1 1024 1

Greece - 2019 64 Table Antimicrobial susceptibility testing of Salmonella Infantis in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 2 <=0.03 2 <=0.25 2 2 2 <=0.5 2 1 <=1 2 2 <=2 2 2 1 <=4 2 4 1 <=8 2 2 8 1

Greece - 2019 65 Table Antimicrobial susceptibility testing of Salmonella Infantis in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 1

Greece - 2019 66 Table Antimicrobial susceptibility testing of Salmonella Infantis in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 2 <=0.03 2 <=0.25 2 2 2 <=0.5 2 2 <=1 2 2 <=2 2 <=4 2 4 2 <=8 2 1 128 1

Greece - 2019 67 Table Antimicrobial susceptibility testing of Salmonella Kedougou in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 64 1

Greece - 2019 68 Table Antimicrobial susceptibility testing of Salmonella Kentucky in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 128 1

Greece - 2019 69 Table Antimicrobial susceptibility testing of Salmonella Kottbus in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 <=8 1 8 1 256 1

Greece - 2019 70 Table Antimicrobial susceptibility testing of Salmonella Livingstone in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 3 0 0 0 1 0 0 0 0 s N <=0.03 3 <=0.25 3 3 3 <=0.5 3 3 0.5 2 <=1 2 3 1 1 <=2 3 2 1 4 1 <=8 3 1 8 2 16 2 32 1 1 128 1

Greece - 2019 71 Table Antimicrobial susceptibility testing of Salmonella Mishmarhaemek in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 1

Greece - 2019 72 Table Antimicrobial susceptibility testing of Salmonella Montevideo in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 1 0 0 0 0 0 0 0 0 s N <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 0.5 1 <=1 1 1 <=2 1 4 1 <=8 1 1 16 1

Greece - 2019 73 Table Antimicrobial susceptibility testing of Salmonella Muenchen in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 64 1

Greece - 2019 74 Table Antimicrobial susceptibility testing of Salmonella Muenchen in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 1 1 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=4 1 4 1 <=8 1 64 1 >1024 1

Greece - 2019 75 Table Antimicrobial susceptibility testing of Salmonella Muenster in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 128 1

Greece - 2019 76 Table Antimicrobial susceptibility testing of Salmonella Senftenberg in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 5 0 0 0 2 0 0 0 0 s N <=0.03 5 <=0.25 5 4 3 0.25 3 <=0.5 5 5 0.5 2 <=1 3 5 1 1 1 <=2 4 2 2 1 4 4 1 <=8 5 1 8 1 16 3 32 2 1 64 2 256 1

Greece - 2019 77 Table Antimicrobial susceptibility testing of Salmonella Szentes in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: HACCP and own check Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 1 <=4 1 <=8 1 1

Greece - 2019 78 Table Antimicrobial susceptibility testing of Salmonella Szentes in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 1

Greece - 2019 79 Table Antimicrobial susceptibility testing of Salmonella Thompson in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: environmental sample - dust Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 1 0 0 0 0 0 0 0 0 s N <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 0.5 1 <=1 1 1 <=2 1 4 1 <=8 1 1 16 1

Greece - 2019 80 Table Antimicrobial susceptibility testing of Salmonella Typhimurium, monophasic in Meat from pig - carcase

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 1 0 0 0 1 0 0 0 0 0 1 1 0 1 s N <=0.015 1 <=0.03 1 <=0.25 1 <=0.5 1 1 <=1 1 1 1 <=4 1 8 1 >32 1 >64 1 1 >128 1 >1024 1

Greece - 2019 81 Table Antimicrobial susceptibility testing of Salmonella Umbilo in Gallus gallus (fowl) - broilers - before slaughter

Sampling Stage: Farm Sampling Type: environmental sample - boot swabs Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 1 0 0 0 0 0 0 0 0 0 1 0 0 1 s N <=0.015 1 <=0.03 1 <=0.25 1 1 <=0.5 1 1 <=1 1 <=2 1 <=4 1 4 1 <=8 1 >32 1 >64 1 >1024 1

Greece - 2019 82 Table Antimicrobial susceptibility testing of Salmonella Umbilo in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 1

Greece - 2019 83 Table Antimicrobial susceptibility testing of Salmonella Uno in Gallus gallus (fowl) - laying hens

Sampling Stage: Farm Sampling Type: animal sample - faeces Sampling Context: Control and eradication programmes Sampler: Official sampling Sampling Strategy: Census Programme Code: AMR MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 <=4 1 4 1 <=8 1 1

Greece - 2019 84 ANTIMICROBIAL RESISTANCE TABLES FOR INDICATOR ESCHERICHIA COLI

Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON pnl2

Analytical Method:

Country of Origin: Italy

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Positive/Pres Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent Ceftazidime Positive/Pres Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.06 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 2 2 2 2 2 2 2 2 2 2 N of resistant

Metric MIC isolates 2 2 0 0 2 0 0 0 0 0 s N <=0.015 2 <=0.03 2 <=0.064 2 <=0.12 1 0.25 2 1 2 1 4 1 1 8 1 1 1 16 1 >32 1 >64 2 Greece - 2019 85 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Italy

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 2 2 2 2 2 2 2 2 2 2 2 2 2 2 N of resistant

Metric MIC isolates 2 1 2 2 2 2 0 1 0 1 2 2 0 2 s N <=0.03 2 <=0.25 2 0.25 1 <=0.5 1 <=1 2 <=4 1 >4 2 8 1 2 1 32 1 >32 1 2 64 1 >64 2 1 >128 2 1 >1024 2

Greece - 2019 86 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Analytical Method:

Country of Origin: Romania

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent Ceftazidime Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.06 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 1 1 1 1 1 1 0 0 0 0 s N <=0.03 1 0.03 1 <=0.12 1 0.25 1 4 1 1 8 1 1 1 64 1

Greece - 2019 87 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Romania

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 1 0 1 1 1 1 0 1 0 1 1 1 0 0 s N <=0.03 1 <=0.25 1 0.25 1 0.5 1 <=1 1 4 1 1 8 1 >32 1 >64 1 1 128 1 >128 1 >1024 1

Greece - 2019 88 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Analytical Method:

Country of Origin: France

Sampling Details:

Metric MIC isolates 4 4 0 0 4 0 0 0 0 0 s N <=0.015 3 <=0.03 4 0.03 1 <=0.064 3 <=0.12 2 3 0.25 1 2 0.5 1 4 2 1 1 8 3 2 3 16 1 1 >32 2 >64 4

Greece - 2019 89 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: France

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 4 4 4 4 4 4 4 4 4 4 4 4 4 4 N of resistant

Metric MIC isolates 4 0 4 4 0 4 0 0 0 4 3 3 0 3 s N <=0.03 4 <=0.25 4 1 <=0.5 3 <=1 4 <=2 1 2 1 1 4 2 1 2 >4 4 <=8 4 8 2 1 >8 1 2 32 1 >32 3 >64 4 3 >128 4 >1024 3

Greece - 2019 90 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 3 3 0 0 3 0 0 0 0 0 s N <=0.015 3 <=0.03 3 <=0.064 3 <=0.12 3 3 1 1 2 1 4 2 3 2 8 1 1 1 64 2 >64 1

Greece - 2019 91 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 3 3 3 3 3 3 3 3 3 3 3 3 3 3 N of resistant

Metric MIC isolates 3 1 3 3 3 1 0 1 0 0 2 3 0 1 s N <=0.015 2 <=0.03 2 0.064 1 <=0.25 3 2 <=0.5 2 0.5 1 <=1 3 1 1 2 1 <=4 2 4 1 1 >4 3 8 1 16 1 32 1 >32 1 1 64 1 1 >64 3 1 1 >128 3 >1024 2

Greece - 2019 92 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Sampling Stage: Slaughterhouse Sampling Type: animal sample - caecum Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: AMR MON pnl2

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Positive/Pres Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent Ceftazidime Positive/Pres Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ent ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.06 0.25 0.064 0.5 0.25 0.12 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 128 2 16 16 128 N of tested isolates 2 2 2 2 2 2 2 2 2 2 2 N of resistant

Metric MIC isolates 2 2 0 0 2 0 0 0 0 0 0 s N <=0.015 1 <=0.03 2 0.03 1 <=0.12 2 0.12 2 0.25 1 0.5 1 1 1 4 1 8 1 1 1 16 2 1 >64 2

Greece - 2019 93 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 146 146 146 146 146 146 146 146 146 146 146 146 146 146 N of resistant

Metric MIC isolates 96 6 2 2 68 26 5 7 0 13 99 119 0 85 s N <=0.015 113 <=0.03 146 0.03 5 0.064 2 0.12 3 <=0.25 144 145 52 0.25 8 <=0.5 144 97 0.5 8 1 8 <=1 4 141 1 1 2 35 1 <=2 42 27 2 31 7 <=4 120 4 15 51 1 4 1 1 >4 2 <=8 77 26 8 44 1 1 11 >8 4 16 1 3 1 2 2 10 32 1 2 9 1 9 2

Greece - 2019 94 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 146 146 146 146 146 146 146 146 146 146 146 146 146 146 N of resistant

Metric MIC isolates 96 6 2 2 68 26 5 7 0 13 99 119 0 85 s N >32 4 84 64 1 14 2 2 26 >64 93 4 91 128 21 2 >128 24 8 >1024 99

Greece - 2019 95 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Positive/Pres Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ent Ceftazidime Positive/Pres Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ent ECOFF 0.125 0.25 0.25 0.25 8 0.5 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.06 0.25 0.064 0.064 0.5 0.25 0.12 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 64 128 128 128 2 16 16 128 N of tested isolates 60 60 60 60 60 60 60 60 60 60 60 60 N of resistant

Metric MIC isolates 57 60 5 5 6 59 5 5 0 0 0 0 s N <=0.015 51 <=0.03 60 0.03 6 <=0.064 43 0.064 3 <=0.12 30 1 47 0.12 3 10 0.25 1 2 17 12 0.5 1 1 7 1 1 3 2 2 3 1 3 2 8 1 4 6 2 31 11 2 11 8 10 1 2 21 18 1 27 16 9 2 1 13 2 21 Greece - 2019 96 AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Positive/Pres Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ent Ceftazidime Positive/Pres Negative/Abs Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ent ECOFF 0.125 0.25 0.25 0.25 8 0.5 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.06 0.25 0.064 0.064 0.5 0.25 0.12 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 64 128 128 128 2 16 16 128 N of tested isolates 60 60 60 60 60 60 60 60 60 60 60 60 N of resistant

Metric MIC isolates 57 60 5 5 6 59 5 5 0 0 0 0 s N 32 14 5 6 >32 10 64 8 4 1 >64 41 1

Greece - 2019 97 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 60 60 60 60 60 60 60 60 60 60 60 60 60 60 N of resistant

Metric MIC isolates 60 8 60 59 24 33 3 4 0 16 46 48 0 44 s N <=0.015 20 <=0.03 60 0.03 3 0.064 4 0.12 2 <=0.25 57 16 0.25 8 <=0.5 1 36 0.5 10 3 <=1 56 1 1 1 18 <=2 1 12 2 9 1 1 2 <=4 25 4 19 4 13 3 >4 56 <=8 36 4 8 31 18 2 12 >8 18 9 16 1 2 7 4 2 32 2 1 2 3

Greece - 2019 98 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 60 60 60 60 60 60 60 60 60 60 60 60 60 60 N of resistant

Metric MIC isolates 60 8 60 59 24 33 3 4 0 16 46 48 0 44 s N >32 1 44 64 2 2 2 3 23 >64 58 4 23 128 10 2 >128 12 12 >1024 46

Greece - 2019 99 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from pig - fresh

Analytical Method:

Country of Origin: Netherlands

Sampling Details:

Metric MIC isolates 2 2 0 0 2 0 0 0 0 0 s N <=0.03 2 0.03 2 <=0.064 2 <=0.12 1 1 0.25 1 1 4 1 8 1 1 1 16 1 32 1 >32 2 >64 2

Greece - 2019 100 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from pig - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Netherlands

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 2 2 2 2 2 2 2 2 2 2 2 2 2 2 N of resistant

Metric MIC isolates 2 0 2 2 2 2 0 1 0 2 2 2 0 2 s N <=0.03 2 <=0.25 2 <=1 1 2 1 1 4 1 >4 2 8 2 >8 1 2 >32 1 2 >64 2 2 >128 2 2 >1024 2

Greece - 2019 101 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from pig - fresh

Analytical Method:

Country of Origin: Greece

Sampling Details:

Metric MIC isolates 11 11 0 0 11 0 0 0 0 0 s N <=0.015 9 <=0.03 11 0.03 2 <=0.064 7 <=0.12 6 9 0.12 4 0.25 3 2 0.5 2 1 1 2 1 1 2 4 1 3 1 8 2 7 2 7 16 1 1 4 2 32 1 1 1 Greece - 2019 102 AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Positive/Pres Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent Ceftazidime Positive/Pres Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ent ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.06 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 11 11 11 11 11 11 11 11 11 11 N of resistant

Metric MIC isolates 11 11 0 0 11 0 0 0 0 0 s N >32 5 >64 10 128 1

Greece - 2019 103 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from pig - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - EFSA specifications Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Greece

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 11 11 11 11 11 11 11 11 11 11 11 11 11 11 N of resistant

Metric MIC isolates 11 3 11 11 10 8 1 0 0 5 8 8 0 8 s N <=0.015 3 <=0.03 11 <=0.25 11 2 0.25 1 <=0.5 9 0.5 2 1 <=1 10 1 1 2 <=2 3 2 2 <=4 5 4 3 1 1 >4 11 <=8 1 8 1 3 1 >8 5 4 16 4 32 1 3 1 >32 8 64 1 >64 11 1 7

Greece - 2019 104 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 11 11 11 11 11 11 11 11 11 11 11 11 11 11 N of resistant

Metric MIC isolates 11 3 11 11 10 8 1 0 0 5 8 8 0 8 s N 128 2 >128 8 5 >1024 8

Greece - 2019 105 OTHER ANTIMICROBIAL RESISTANCE TABLES

Greece - 2019 106 Specific monitoring of ESBL-/AmpC-/carbapenemase-producing bacteria and specific monitoring of carbapenemase-producing bacteria, in the absence of isolate detected

Total Total Programme Matrix Zoonotic Agent Sampling Sampling Sampling Sampling Metrics Units Units Code Detailed Detailed Strategy Stage Details Context Sampler Sample Type Sampling Unit Type Sample Origin Comment Tested Positive CARBA Meat Escherichia Objective Retail N_A Monitorin Official food sample - batch (food/feed) Belgium N_A 1 0 MON from coli, non- sampling g - EFSA samplin meat Denmark N_A 4 0 bovine pathogenic, specificat g animals - unspecified ions France N_A 32 0 fresh Germany N_A 5 0 Greece N_A 59 0 Ireland N_A 2 0 Italy N_A 9 0 Netherlands N_A 10 0 Poland N_A 2 0 Romania N_A 2 0 Spain N_A 5 0 Meat Escherichia Objective Retail N_A Monitorin Official food sample - batch (food/feed) Belgium N_A 1 0 from pig - coli, non- sampling g - EFSA samplin meat Denmark N_A 2 0 fresh pathogenic, specificat g unspecified ions France N_A 11 0 Germany N_A 4 0 Greece N_A 139 0 Netherlands N_A 57 0 Spain N_A 2 0 Pigs - Escherichia Objective Slaughte N_A Monitorin Official animal slaughter animal Greece N_A fattening coli, non- sampling rhouse g - EFSA samplin sample - batch 153 0 pigs pathogenic, specificat g caecum unspecified ions

Greece - 2019 107 Specific monitoring of ESBL-/AmpC-/carbapenemase-producing bacteria and specific monitoring of carbapenemase-producing bacteria, in the absence of isolate detected

Greece - 2019 108 Latest Transmission set

Last submitted Metrics dataset Table Name transmission date Antimicrobial Resistance 24-Jul-2020 Esbl 24-Jul-2020 Animal Population 24-Jul-2020 Disease Status 24-Jul-2020 Food Borne Outbreaks 24-Jul-2020 Prevalence 19-Jan-2021

Greece - 2019 109 Greece, Text Forms 2019

Table of contents

1. Institutions and Laboratories involved in zoonoses monitoring and reporting ...... 3

2. Animal population ...... 3

3. General evaluation: Flavivirus ...... 3

4. Description of Monitoring/Surveillance/Control programmes system: Equines-Flavivirus ...... 3

5. Description of Monitoring/Surveillance/Control programmes system: All animals, Verocytotoxic E.coli ...... 6

6. General evaluation: Rabies virus ...... 6

7. Description of Monitoring/Surveillance/Control programmes system: All animals-Rabies virus ...... 7

8. General evaluation: Thermophilic Campylobacter ...... 9

9. Description of Monitoring/Surveillance/Control programmes system: All animals-Listeria monocytogenes ...... 10

10. Description of Monitoring/Surveillance/Control programmes system: All foodstuffs-Listeria monocytogenes ...... 10

11. Description of Monitoring/Surveillance/Control programmes system: All domestic farmed and wild swine eligible for slaughter, Trichinellosis, Trichinella spp ...... 11

12. Description of Monitoring/Surveillance/Control programmes system: All animals-Toxoplasma ...... 11

13. Description of Monitoring/Surveillance/Control programmes system: Fishery products from fish species associated with large amounts of histamine-Histamine ...... 12

14. General evaluation: Echinococcus ...... 12

15. General evaluation: Coxiella burnetii ...... 13

16. Description of Monitoring/Surveillance/Control programmes system: Sheep and goats-Coxiella burnetii ...... 13

17. General evaluation: Brucella melitensis ...... 15

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18. Description of Monitoring/Surveillance/Control programmes system: Sheep & goats. Brucella melitensis ...... 16

19. General evaluation: Mycobacterium tuberculosis complex ...... 18

20. Description of Monitoring/Surveillance/Control programmes system: Cattle (bovine animals). Mycobacterium tuberculosis complex ...... 18

21. General evaluation: Salmonella spp ...... 20

22. Description of Monitoring/Surveillance/Control programmes system: Gallus gallus (fowl) and Turkeys-Salmonella spp ...... 21

23. Description of Monitoring/Surveillance/Control programmes system: All foodstuffs-Salmonella spp...... 24

24. General evaluation: Brucella abortus ...... 25

25. Description of Monitoring/Surveillance/Control programmes system: Cattle (bovine animals)- Brucella abortus ...... 26

26. Food-borne Outbreaks ...... 27

27. Institutions and laboratories involved in antimicrobial resistance monitoring and reporting ...... 28

28. General Antimicrobial Resistance Evaluation ...... 28

29. General Description of Antimicrobial Resistance Monitoring; Caecal sample from fattening pigs / Indicator commensal E. Coli ...... 28

30. General Description of Antimicrobial Resistance Monitoring; Caecal sample from fattening pigs / ESBL-/AmpC-/Carbapenamase- producing E.Coli 30

31. General Description of Antimicrobial Resistance ; Fresh pig and bovine meat samples / ESBL-/AmpC-/Carbapenamase producing E.coli ...... 31

32. General Description of Antimicrobial Resistance Monitoring; Pig carcasses / Salmonella ...... 32

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1. Institutions and Laboratories involved in zoonoses monitoring and reporting The authority, responsible for collecting data on zoonoses, zoonotic agents and antimicrobial resistance is the Department of Zoonoses of the Animal Health Directorate of the General Veterinary Directorate of the Ministry of Rural Development and Food (MRDF). Department of Zoonoses drafts the annual zoonoses report and submit it to EFSA in close collaboration with the Department for the support of E-governance of the Directorate of E-Governance of the MRDF. Sources of collected data are:

 the two Directorates of Veterinary Institutes of the MRDF with their 21 departments of state veterinary laboratories  the laboratories of the Hellenic Food Authority (EFET)  the Institute of Technology of Agricultural Products of MRDF and  the Veterinary Departments of the country’s regional units. The source of information regarding foodborne outbreaks (FBO) is the Hellenic National Public Health Organisation called EODY (previously called KE.EL.P.NO).

2. Animal population

1. Sources of information and the date(s) (months, years) the information relates to For pig, cattle, sheep and goat population data, information comes from the computerized data-based system of the Hellenic Ministry of Rural Development and Food. For fish population, data comes from the Directorate General of Fisheries. For other animal species population data comes from the Regional Veterinary Authorities.

3. General evaluation: Flavivirus

1. Evaluation of status, trends and relevance as a source for humans Data on human cases originate from the "Hellenic National Public Health Organisation" (EODY) of the Ministry of Health.

4. Description of Monitoring/Surveillance/Control programmes system: Equines-Flavivirus 1. Monitoring/Surveillance/Control programmes system Since 2010, a surveillance programme for WNF is in place in Greece. West Nile Fever is a disease of mandatory declaration. Every suspect case, reported by official veterinarians, private practitioners or owners of equines, is investigated by the Competent Veterinary Authorities (passive surveillance). Every febrile equine with or without neurological signs or/and mobility disorders is considered to be a suspect case and is a subject of blood sampling for further laboratory evaluation. Official veterinarians are responsible for the blood sampling and the dispatch of the samples to the authorized state laboratory. All samples are examined according to the methodology described in Chapter 2.1.20 – West Nile Fever – b. Diagnostic techniques of the OIE Terrestrial Animals Manual. Greece 3

When a suspect case is confirmed as positive, it must be reported without any delay to the Central Competent Authority, i.e. The Department of Infectious and Parasitic Diseases of the Animal Health Directorate, Ministry of Rural Development and Food (MRDF). The dispatches of suspect cases and other equines of the same holding are forbidden until the completion of the laboratory tests and of the epidemiological survey. The West Nile Fever (WNF) surveillance programme of Greece consists of active surveillance in equine and wild avian populations and passive surveillance in wild and domestic birds, in Equidae and in other domestic animals sensitive to WNF. The purpose of this programme is to protect public and animal health by determining the origin and the possible reservoirs of the causative agent (West Nile Virus), which areas of Greece are endemic or of high risk for an outbreak of an epizootic/epidemic and the appropriate preventive measures for the spread of an outbreak of the disease.

Passive surveillance in avian and equine populations:

Dead wild avian populations and other birds (breeding, pets) as well as samples from equine populations or domestic carnivores sensitive to the WNF virus (tested on a case-by-case basis) are collected and sent to the authorised laboratories for testing, throughout the year. All samples are examined according to the methodology described in Chapter 2.1.20 – West Nile Fever – b. Diagnostic techniques of the OIE Terrestrial Animals Manual. All relevant data and information are reported to the Central Competent Authority, i.e. The Department of Infectious and Parasitic Diseases of the Animal Health Directorate, MRDF.

Active surveillance in equines:

The programme of active surveillance in equines for WNF in Greece involves: A. The clinical examination and laboratory evaluation, as well as the control of present and the investigation of previous dispatches of equines from holdings: a. within a zone of 20km from any positive human or animal case and b. in neighbouring regional units (RUs) in a distance of 20 km from the borders with affected RUs. Data on human cases come from the "Hellenic National Public Health Organisationn" (EODY) of the Ministry of Health B. The periodic serological examination of sentinel horses that are selected in a strategic manner so as to obtain surveillance results that are representative of the whole country. The criteria for the selection of sentinel horses include: a. geographical criteria, b. measures for the control of insects populations (holdings and animals with no application of insecticides or insects repellents are selected), c. movements (horses that are free to move only within a radius of 20km from their holding are selected), e. keeping conditions (horses that periodically remain out of stable establishments are selected). Sentinel horses get sampled 3 times during the period from mid-May until the end of September, with minimum intervals of 3 weeks. All samples are dispatched to the authorized laboratory and according to the methodology described in Chapter 2.1.20 – West Nile Fever – b. Diagnostic techniques of the OIE Terrestrial Animals Manual All relevant data and information are reported to the Central Competent Authority, i.e. The Department of Infectious and Parasitic Diseases of the Animal Health Directorate, MRDF.

Active surveillance in wild birds:

Blood sampling of wild avian populations from selected areas (mostly water habitats) of the Greek mainland (specified number of wild birds per species – Pica pica, Corvus cornix, Sturnus vulgaris, Turdus merula, other species – and sampling site). The samples are dispatched to the authorised laboratory and examined according to the methodology described in Chapter 2.1.20 – West Nile Fever – b. Diagnostic techniques of the OIE Terrestrial Animals Manual. All relevant data and information are reported to the Central Competent Authority, i.e. The Department of Infectious and Parasitic Diseases of the Animal Health Directorate, MRDF.

Laboratory examinations in equines

The analytical methods used are: Greece 4

a. ID VET SCREEN COMPETITION ELISA and b. POURQUIER IgM or ID VET IgM CAPTURE ELISA for the differentiation of recent to past antibodies in all samples with a positive ELISA result.

2. Measures in place In the framework of the “National Programme of WNF surveillance in equines and wild birds”, the active surveillance in equines consists of:

1.The clinical examination and laboratory evaluation of equines from holdings: a. within a zone of 20 km from any positive human or animal case and b. in neighbouring regional units in a distance of 20 km from the borders with the affected RU. Data on human cases come from the "Hellenic National Public Health Organisation" (EODY) of the Ministry of Health.

2. The periodic serological examination of sentinel horses that are selected in a strategic manner to obtain surveillance results that are representative of the whole country. Sentinel horses get sampled 3 times during the period from mid-May until the end of September, with minimum intervals of 3 weeks. 3. Notification system in place to the national competent authority West Nile Fever is a disease of mandatory declaration. Every suspect case, reported by official veterinarians, private practitioners or owners of equines, is investigated by the Competent Veterinary Authorities (passive surveillance). Every febrile equine with or without neurological signs or/and mobility disorders is considered to be a suspect case and is a subject of blood sampling for further laboratory evaluation. Official veterinarians are responsible for the blood sampling and the dispatch of the samples to the authorized state laboratory. All samples are examined according to the methodology described in Chapter 2.1.20 – West Nile Fever – b. Diagnostic techniques of the OIE Terrestrial Animals Manual.

All of the outbreaks have been also notified to the Competent Authorities of Public Health and to the ADNS and WAHIS (OIE). 4. Results of investigations and national evaluation of the situation, the trends and sources of infection Laboratory examinations in equines

The analytical methods used are: a. ID VET SCREEN COMPETITION ELISA and b. POURQUIER IgM or ID VET IgM CAPTURE ELISA for the differentiation of recent to past antibodies in all samples with a positive ELISA result.

For 2019 please see below the laboratory results:

Equidae (active & passive surveillance, other reasons)

 IDVet Screen competition (IgG antibodies, previous infection):

 1082 tests, from which 252 IgG positive and 4 doubtful

 D VET IgM CAPTURE (IgM antibodies, Recent infection)::

 443 tests, from which 24 positive, 1 doubtful

 Real-Time RT-PCR: 53 tests with one positive

Avian populations

• In total, samples from 29 birds were tested: Greece 5

-17 dead birds -12 live birds • real time RT-PCR tests were performed in samples from 29 birds: 1 positive

The total number of WNV outbreaks in 2019 was 22; 21 in equine and 1 in birds.

5. Description of Monitoring/Surveillance/Control programmes system: All animals, Verocytotoxic E.coli 1. Monitoring/Surveillance/Control programmes system

There is no official National monitoring program in force for detecting VTEC serovars in animals and food.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection

Results of investigations in the year 2019: Several food samples and animal species were tested for E.coli spp in 2019 as shown in the EFSA tables.

6. General evaluation: Rabies virus

1. History of the disease and/or infection in the country The last rabies case in animals was detected in the Evros prefecture in 1987. Nevertheless, the reoccurrence of the disease in Greece in 2012 could not be characterized as unexpected due to several observations. At first, there was a high prevalence of the disease in neighboring countries such as Turkey, Bulgaria, Albania and FYROM. This supported concerns for trans-border rabies spread in Greece. Taking into consideration this situation, in 2012, the pre-existing national program for rabies passive surveillance according to Directive 99/2003/EC was enhanced imposing the collection of all dead and suspected for rabies animals from 16 regional units along the northern and eastern borders of the country. In October 2012 rabies was diagnosed in a red fox (Vulpes vulpes) in Kozani regional unit. By the end of 2012 nine positive cases were confirmed by the National Reference Laboratory for rabies. The partial sequencing analysis and the subsequent phylogenetic analysis supported the hypothesis of movement of rabies-infected hosts in Western Balkan countries. To address the epidemic and prevent its spread, a Greek National Rabies control and eradication programme was implemented based on the passive surveillance of the disease. Following the identification of the infected fox, the collection of samples from dead animals was extended on the entire country with a main aim to achieve a more efficient surveillance of the disease. The mandatory vaccination of all dogs and cats, the management of all rabies– suspect animals and the control in animal movements were other imposed measures for the prevention of the disease. Until 26 May 2020, in the framework of the passive surveillance of the disease, a total of 48 rabid animals have been confirmed by the National Reference Laboratory for Rabies in animals (NRL), which is the Virology Laboratory in Athens Veterinary Center. The last rabies case was confirmed in a red fox in Pella regional unit in May 2014. In detail, nine (7 foxes, 2 dogs), twenty-nine (25 foxes, 1 dog, 1 cat, 2 cattle) and ten (8 foxes, 2 dogs) rabies cases were laboratory confirmed in 2012, 2013 and 2014 respectively. Since then no new cases have been recorded. There is no reliable data source in Greece concerning animal wildlife population (e.g. Red Foxes). Greece 6

3. Any recent specific action in the Member State or suggested for the European Union

The increase of the remuneration for the monitoring samples provided to hunters/game keepers from 30 to 50 EUROS since 2017 further motivated game keepers/hunters for the collection of samples and this led to an increase in sample size.

Since July 2017, Department of Zoonoses, in close collaboration to the Ministry of Environment and Energy, investigated other solutions that could increase the delivery of samples (more flexible missions, increasing number of missions, use of specific equipment for attracting foxes et.

In addition, a new tv spot has been produced in order to increase the public awareness in an effort to improve the surveillance and the procedure of notification to the competent authorities of the animals that are found dead by the general public.

In the following link, please find all actions taken by the Greek Competent Authorities, in regards with the recommendations following the EU audit rabies in 2017. http://ec.europa.eu/food/audits-analysis/audit_reports/details.cfm?rep_id=3912

7. Description of Monitoring/Surveillance/Control programmes system: All animals-Rabies virus 1. Monitoring/Surveillance/Control programmes system The main pillars of the programme are:

 Passive surveillance programme.  Oral Rabies Vaccination and monitoring programme  Other measures like the mandatory vaccination of pet animals and other carnivores used as pets.  Control of stray animals (collection, registration, vaccination against rabies).  Control of animal movements in the country (dogs and cats must be vaccinated and properly identified and registered before any travel).  Furthermore, according to the national decision for the implementation of the programmes and in special cases, the CA asks for the vaccination of farm animals against rabies as well.  Close collaboration with the hunting and forestry agents.  Close collaboration with neighbouring countries.  More information can be found in the relevant final report for the implementation of the programme at the following link: https://ec.europa.eu/food/sites/food/files/safety/docs/cff_animal_vet-progs_2018- 9_rabies_grc.pdf

The total number of samples required for the monitoring of the effectiveness of the oral vaccination campaign was 2 red foxes/100 km2 /campaign as it is recommended by WHO and EE guidelines.

In the framework of the passive surveillance program during 2019, brain samples from 790 animals were collected and examined. All these samples were found negative for rabies virus.

Two Oral Vaccination campaigns for the immunization of red foxes against rabies were launched in Greece in Spring and Autumn 2019. A communication campaign (posters and leaflets, a new TV spot, special educational material and courses for children in schools, press releases) has been implemented with the assistance of the Ministry of Education, the Greece 7

regional veterinary services and the students’ associations of the two Veterinary Schools, in order to raise the public awareness with regards to rabies and to inform the public regarding the philosophy of red fox oral vaccination programme, the time schedule of the vaccination strategy, the areas involved and the necessary prophylactic measures to be adopted in order to avoid potentially harmful exposures to the live attenuated vaccine distributed by air. Vaccine-baits were aerially distributed by fixed-wind aircrafts, in 24 regional units of the country in a density of 20-25 baits per km2. The whole procedure was supervised by official veterinarians. The following monitoring program assessing the effectiveness of oral vaccination campaign was based on the examination of serum samples and canine teeth derived from red foxes collected in the field. The initiation of the program in each Regional Unit was performed one month following the end of vaccinations and lasted for all Regional Units more than two months. Despite the difficulties imposed by the COVID-19 pandemic, the 2020 spring Oral Vaccination campaign for the immunization of red foxes against rabies started on the 12th of May 2020.

2. Measures in place

1.In case of detection of new positive cases in animals, the following actions/measures are applied (all these measures are included in the Joint Ministerial Decision for the Control of Rabies in animals in Greece): -Management of other animals that were in contact with the confirmed rabid animal (depending on the vaccination status for the domestic dogs-cats) - culling of livestock that was in contact with a confirmed rabid animal -Management of the products produced by the rabid animal (livestock) -Processing of the bedding, and the manure and waste in herds-flocks -Disinfection of the premises (holding et -Placement of informative panels for the public awareness in an area where rabies cases have been detected -Restrictions in the movements of sensitive animals to the rabies virus from/to the affected areas -In case of increase number of rabies cases in wildlife in an area, the possibility of elimination of the fox population may be examined or we may include new vaccination areas in our programme.

2. Passive surveillance program. In 2019, 790 animals were collected in the framework of passive surveillance. The number of collected animals per species and regional unit and the laboratory results can be found in the reporting prevalence tables. There were no positive results for rabies.

3. Oral Vaccination campaigns (2 per year) and monitoring. In 2019 and in the framework of the monitoring following the spring and autumn vaccination campaigns, 286 red foxes were collected (sampling collection and analyses remain still in progress). Sero-positivity levels and TTC intake are considered satisfactory.

3. Notification system in place to the national competent authority Rabies is a notifiable disease. Each person or professional involved in the management of animals is obliged to notify any suspect symptom recorded in animals to the competent veterinary authorities. The legal basis for this is the Presidential Decree 133/1992 and the Joint Ministerial Decision for the Implementation of the Rabies Program in Greece (Number: 331/10301-25/01/2013 (FEK 198 B').

4. Results of investigations and national evaluation of the situation, the trends and sources of infection Regarding the passive surveillance program, brain samples from 790 animals were collected during 2019. All these samples were negative for rabies virus. Furthermore, two Oral Vaccination campaigns for the immunization of red foxes against rabies were launched in Greece in Spring and Autumn 2019. A communication campaign (posters and leaflets, a new TV spot, special educational material and courses for children in Greece 8

schools, press releases) has been implemented with the assistance of the Ministry of Education, the regional veterinary services and the students’ associations of the two Veterinary Schools, in order to raise the public awareness with regards to rabies and inform the public regarding the philosophy of red fox oral vaccination programme, the time schedule of the vaccination strategy, the areas involved and the necessary prophylactic measures to be adopted in order to avoid potentially harmful exposures to the live attenuated vaccine distributed by air. Vaccine-baits were aerially distributed by fixed-wind aircrafts in 24 regional units of the country (20 -25 baits per km2). Vaccines were dropped along parallel flight paths 500 m apart from each other, in order to optimize aerial dropping and achieve homogeneous distribution. The whole dropping process has been conducted and monitored using a special automatic device equipped with GPS receiver, provided by the baits manufacturer. This equipment was installed in each aircraft, in order to register the dropping coordinates of each bait and send the records in “real-time” both to the competent authority and the aerial distribution company. The whole procedure was supervised by Official Veterinarians of the Regional Units involved in the campaigns. The following monitoring program assessing the effectiveness of oral vaccination campaign was based on the examination of serum samples and canine teeth derived from red foxes collected in the field. The initiation of the program in each regional unit was performed one month following the end of vaccinations and lasted for all regional units more than two months. The collection of samples was finally completed for the spring ORV 2019 campaign at the end of September 2019 (just before the new vaccination campaign of autumn 2019) and at the end of March 2020 for the campaign of autumn 2019 ORV. The total number of samples required for the monitoring of the effectiveness of the oral vaccination campaign was 2 red foxes/100 km2/campaign as it is recommended by WHO.

For the monitoring performed after the completion of the spring 2019 ORV, samples that came from 191 hunted foxes have been collected. The analysis in the lab revealed TTC detection in 86 out of 190 samples tested (45.26%). From the 170 blood samples tested, 69 were seropositive (40.59%). The great majority of samples collected, derived by the young fox population, a fact that may account for the relatively low rates of TTC uptake, in compliance with previous spring ORV campaigns. For the monitoring following the autumn ORV 2019, (sampling collection is still in progress), samples from 270 red foxes were hunted. Based on the available data, detection of TTC uptake was 93% and seropositivity rates were at a percentage of 64.8%. All the brain samples have been already examined with FAT and found negative.

Taking into consideration the absence of positive samples since May 2014 and the enhancement of passive surveillance as well as the ORV campaigns that are carried out continually since 2013, the trends is towards the elimination of the disease in the Greek territory. However, regarding the epidemiological situation in certain neighboring countries, awareness and monitoring programmes should be continued. Oral Rabies Vaccination Campaigns are proposed to be continued as well, especially on the north-eastern borders.

5. Additional information All relevant information with regard to rabies can be retrieved at the following link: http://www.minagric.gr/index.php/el/for-citizen-3/nosimata-zoon/457-lissa

8. General evaluation: Thermophilic Campylobacter

1. History of the disease and/or infection in the country In 2014 and 2018, a monitoring programme took place in broiler flocks Gallus gallus, in fattening turkeys and in their products, according to the requirements of CD 2013/652/EU. The results are presented in the relevant EFSA tables.

In 2019, 33 herds of pigs were monitored for Campylobacter according to EFSA specifications Greece 9

and 14 detected positive for Campylobacter jejuni. Additionally, 60 slaughter animal batches from broilers were tested according to the requirements of Reg. (EU) No 2073/2005, six detected positive for Campylobacter coli and 13 for Campylobacter spp.

9. Description of Monitoring/Surveillance/Control programmes system: All animals-Listeria monocytogenes 1. Monitoring/Surveillance/Control programmes system Surveillance system: Official sampling is performed by the national veterinary authorities within the framework of clinical investigations. Summary results of 2018 are presented in the relevant EFSA tables.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection Percentage % of positive samples among tested units for Listeria monocytogenes :Animals (sheep and Goats) 20%, while in 2017 it was 46,15%, in 2016 35,5%, in 2015 53,12%, in 2014 1,03%, in 2013 18,75% and in 2012 the same percentage was 42%. Here, it should be noted that the collection of these samples was carried out within the framework of the clinical investigation of herds which reported to the competent veterinary services incidents of death in their livestock. Bovine and pig animals tested for Listeria in 2018 were negative.

10. Description of Monitoring/Surveillance/Control programmes system: All foodstuffs-Listeria monocytogenes 1. Monitoring/Surveillance/Control programmes system Food Surveillance system: Routine and targeted official sampling performed by the national veterinary public health authorities and the Hellenic Food Safety Authority (EFET) respecting the microbiological criteria foreseen by Community Legislation and Hygiene Package. Methods used: the laboratory methods used for Listeria detection and enumeration were: ISO 11290-1 for detecting L. monocytogenes (ISO, 1996, 20171 and ISO 290 11290-2 for enumeration of L. monocytogenes (ISO, 1998, 2017d), in accordance with regulation (E No 2073/2005 on microbiological criteria for foodstuffs. Summary results of 2019 are presented in the relevant EFSA tables.

The overall reported and calculated percentage of Listeria positive findings (units) in all tested food samples was 0.10% in 2018, while in 2017 it was 0,56%, in 2016 it was 0,95% % and in 2015 it was 1,5% for all food categories examined.

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11. Description of Monitoring/Surveillance/Control programmes system: All domestic farmed and wild swine eligible for slaughter, Trichinellosis, Trichinella spp 1. Monitoring/Surveillance/Control programmes system Compulsory examination for detection of Trichinellosis at Slaughterhouse level.

Method used: The targeted animals were examined by the official reference method of Trichinella detection as foreseen and described in Annex I Chapter I of Commission Implementing Regulation (EU) 2015/1375 laying down specific rules on official controls for Trichinella in meat (Magnetic stirrer method for pooled sample digestion).

4. Results of investigations and national evaluation of the situation, the trends and sources of infection Epidemiological history Data on Trichinella spp. unspecified coming from the meat inspection activities are presented in the relevant EFSA tables.

No positive findings (Trichinella spp. Unspecified) were reported at slaughterhouse level under the meat inspections activities in 2016, 2017 and 2018.

During the reporting year 2016, 1.101.376 pigs , 1312 wild boars and 777 other animal species were tested for Trichinella spp at slaughterhouse level with negative results.

During the reporting year 2017, 1.032.981 pigs, 1545 wild boars and 304 other animal species were tested for Trichinella spp at slaughterhouse level with negative results.

During the reporting year 2018, 1.030.434 pigs, 1438 wild boars and 516 other animal species were tested for Trichinella spp at slaughterhouse level with negative results.

During the reporting year 2019, 1.040.345 pigs, 2036 farmed wild boars and 4 wild boars were tested for Trichinella spp at slaughterhouse level with negative results (502 of the total were piglets).

The targeted animals were examined by the official reference method of Trichinella detection as foreseen and described in the Annex 1 of the Commission Regulation 2015/1375 (Magnetic stirrer method for pooled sample digestion).

Results of monitoring: No positive findings were detected.

12. Description of Monitoring/Surveillance/Control programmes system: All animals-Toxoplasma 4. Results of investigations and national evaluation of the situation, the trends and sources of infection In 2016, 23 blood sera samples were sent to the appropriate laboratory (13 goat and 10 sheep) in the frame of investigating abortions. The tests detected 1 positive sheep and 5 positive goats. The laboratory method used in 2016 was indirect elisa for the detection of Toxoplasma antibodies in blood sera. The sampling schemes were not random or representative and samples were collected from sheep and goat herds which reported abortions under clinical investigation practices. Results of 2016 monitoring Data are available in the relevant tables of EFSA. In 2017, 23 blood sera samples from sheep and goats were sent to the laboratory. The tests

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detected 4 positive animals. The laboratory method used in 2017 was indirect elisa for the detection of Toxoplasma antibodies in blood sera. The sampling schemes were not random or representative and samples were collected from sheep and goat herds which reported abortions under clinical investigation practices. Results of 2017 monitoring Data are available in the relevant tables of EFSA. In 2018, 174 blood sera samples were collected from sheep, goats and other animals and tested for toxoplasmosis. Laboratory testing detected 174 positive animals. The laboratory method used was Indirect Elisa for the detection of Toxoplasma antibodies in blood sera. The sampling schemes were not random or representative and samples were collected from sheep and goat herds, as well as form other animals which had reported abortions under clinical investigation practices. Results of 2018 monitoring Data are available in the relevant tables of EFSA. In 2019, 40 blood sera samples were collected from sheep, goats and tested for toxoplasmosis. Laboratory testing detected 2 positive animals. The laboratory method used was Indirect Elisa for the detection of Toxoplasma antibodies in blood sera. The sampling schemes were not random or representative and samples were collected from sheep and goat herds which had reported abortions under clinical investigation practices. Results of 2019 monitoring Data are available in the relevant tables of EFSA.

13. Description of Monitoring/Surveillance/Control programmes system: Fishery products from fish species associated with large amounts of histamine-Histamine 1. Monitoring/Surveillance/Control programmes system Histamine in Food Surveillance system: There is no official monitoring program or systematic scheme applied for Histamine in food. Sporadic samples from fish and fishery products are examined in the designated national veterinary laboratory in Thessalonica- Greece and in the lab of EFET. Targeted fish species for testing and detecting Histamine are: Scrombridae, Clupeidae, Engraulidae, Coryfenidae, Pomatomidae and Scrombresosidae. Related Legislation: Community Regulation (E: 1141/2007 and Reg. (E 2073/2005. Results of monitoring Data are presented in the relevant table of EFSA.

14. General evaluation: Echinococcus

2. Evaluation of status, trends and relevance as a source for humans Source of human infection Mainly through the consumption of contaminated raw foodstuffs (i.e vegetables).

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15. General evaluation: Coxiella burnetii

2. Evaluation of status, trends and relevance as a source for humans

Relevance of the findings in animals to findings in foodstuffs and to human cases (as a source of infection)

In 2016, an infected flock (7 positive ELISA samples out of 8 tested) from a region of Peloponnesus was epidemiologically associated with one (1) human confirmed q fever case. An additional flock from the same region was initially strongly suspected from Human prospective (Q positive Human case- farmer) but when the flock was tested the results were negative.

No relevance between Coxiella burnetii seropositive animals (cattle, sheep, goats) and human cases was reported during the period 2017-2019 in Greece.

16. Description of Monitoring/Surveillance/Control programmes system: Sheep and goats-Coxiella burnetii 1. Monitoring/Surveillance/Control programmes system

1.Monitoring system - Sampling strategy

There is no official / National program in place. Targeted monitoring combined with passive surveillance. – Targeted sampling from aborted animals under clinical investigation.

2. Monitoring system - Frequency of the sampling

Sporadic and targeted blood sampling in clinical affected and symptomatic flocks. There is no regular frequency sampling. The frequency of the sampling is associated with passive surveillance related to abortion notifications managed by competent authorities or private vets.

3. Monitoring system - Type of specimen taken

Blood samples and /or sera

4. Monitoring system - Methods of sampling (description of sampling techniques)

Targeted and Selected Sampling. Blood sampling from all new and previously aborted animals in infected flocks. Moreover, blood sampling carried out in flocks under clinical and epidemiological investigation when human cases of Q fever are linked and confirmed.

5. Monitoring system - Case definition

According to the Zoonoses Reporting Manual and EFSA Scientific Reports. Molecular methods (PCR) and culture for confirmation and support the Case definition were not performed.

6. Monitoring system - Diagnostic/analytical methods used

Serological Tests: Commercial ELISA immunoenzymatic assay kit (IDEXX Elisa Kit was used)

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to detect Coxiella burnetii antibodies in blood sera.

7. Vaccination policy

No vaccination strategy-policy or vaccination programmes were officially reported from the competent authorities.

8. Other preventive measures than vaccination in place

Biosecurity measures, Segregation of pregnant animals, burning of aborted materials and reproductive tissues. Restrictions of animal movement between infected and healthy flocks.

2. Measures in place Vaccination policy: No vaccination strategy-policy or vaccination programmes were reported.

Preventive measures

Biosecurity measures: Segregation of pregnant animals, burning of aborted materials and reproductive tissues, Restrictions of animal movement between infected and healthy flocks.

Control programme/mechanisms - The control program/strategies in place: No national control programme is in place.

3. Notification system in place to the national competent authority

Obligatory notifiable disease under the national legislation (Presidential Decree 133/1992).

4. Results of investigations and national evaluation of the situation, the trends and sources of infection Results from sheep and goat herds are presented in EFSA Tables.

Epidemiological history: During the period 2001-2006 Coxiella burnettii was detected in 68 small ruminant flocks and 1 bovine herd. Animal infection rate in affected flocks ranged from 2.1 % to 31.5%. Findings, along with previously conducted studies, indicate the existence of the disease in animals. However, no data from animals or foods exist on a systematic basis. Moreover, based on clinical data and confirmed laboratory results the animal seroprevalence increased the year 2010 (18.30%) compared to the year 2009 (14,29%) and probably is attributed to the significant higher number of tests carried out not only in 2010 and the following years respectively .

The last years is obvious that the farmers are more aware to find out the cause of abortions in their farms without excluding the Coxiella burnettii as a probable source of infection from animal secretions, excretions, and tissues.

Update information and evaluation of the 2016, 2017, 2018 and 2019 situation: Based on laboratory results from all tested regions, the average calculated Coxiella burnetii seroprevalence (seropositivity %) in tested animals was 16.9% (62/367) in 2016, while the proportion of positive sheep and goat flocks in 2016 was 34.2% (13/38). In 2017 and 2018, the average calculated Coxiella burnetii seroprevalence (seropositivity %) in tested animals was 16.07% (27/168) and 20.8% (38/183) respectively. At this point it should be mentioned that samples were collected within the frame of clinical investigation at farm level.

In 2019 the overall flock and animal reported seroprevalence in clinical suspected tested farms was 63.15% and 21.18% respectively. Twelve (12) sheep, goat, and bovine farms out of 19 tested were found positive, whereas 36 animals out of 170 serological tested were Coxiella burnetii positive from various geographical regions in Greece. From epidemiological point of view, Q fever is a more common disease in islands compared to the mainland. Windy weather in islands may facilitate as a risk factor the disease transmission within and between farms.

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17. General evaluation: Brucella melitensis

1. History of the disease and/or infection in the country The Greek programme started in 1977 with the vaccination of all young female sheep and goats with the REV-1 vaccine in whole Greece. Between 1992-1994, it was decided to implement an eradication programme in whole Greece by blood-testing all animals over 6 months-old. During 1998-1999, the improper implementation of the eradication programme and the following increasing numbers of human and animal cases led to the decision to restart the vaccination programme in the mainland of Greece and to continue with the eradication programme in the majority of the Greek islands. In Greece, since 1-1-1999, the Veterinary Service of the Ministry of Rural Development & Food has carried out both a control and an eradication programme. Two different strategies for the control and eradication of brucellosis have been chosen in the mainland and in the islands. In the mainland and in 3 islands (Evia, Lesvos and Thasos), where the prevalence of brucellosis in sheep and goat herds is high, the programme consists of mass vaccination of young and adult female animals (>3months old) and of young male animals (3-6 months old). Moreover, all unvaccinated male animals (>6months old) must be blood-tested annually. In case the male animals were vaccinated during the age of 3-6 months old, they undergo blood-sampling after a minimum period of 12 months. In the islands, where the disease is in low prevalence among sheep and goat herds test and slaughter policy is indicated. All animals over 6 months old must be blood-tested and the positive ones are slaughtered within a month.

2. Evaluation of status, trends and relevance as a source for humans Greece is not an officially brucellosis free country and there are no officially brucellosis free regions. Brucellosis is a notifiable disease. Any suspected or confirmed case of the disease (both in humans or animals) must be reported to the relevant authorities. The disease in humans is mainly attributed to Br. melitensis. Source of human infection: Mainly from animal contact and consumption of dairy products of unknown origin and hygiene quality. Especially, consumption of dairy products prepared from non pasteurized milk or immature types of sheep and goat cheese.

3. Any recent specific action in the Member State or suggested for the European Union Every year the Directorate of Animal Health requests from the European Union the co- financing (purchase of the vaccines) of the Sheep & Goat Brucellosis Programme.

4. Additional information Epidemiological history. Sheep and goat brucellosis due to B. melitensis is a significant disease for both public health and animal production in Greece. During the last years a control and eradication program is running by the veterinary services of the Ministry of Rural Development & Food. The aim of the program is to control the prevalence and incidence of the disease in areas of the country where these estimates are reported high, by vaccination of lambs and kids. At the same time, in the remaining parts of the country, where the prevalence of the disease is reported low among sheep and goat herds, an eradication program is implemented by test and slaughter policy.

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18. Description of Monitoring/Surveillance/Control programmes system: Sheep & goats. Brucella melitensis 1. Monitoring/Surveillance/Control programmes system Two different strategies for the control and eradication of brucellosis have been chosen in the mainland and in the islands. In the mainland and in 3 islands (Evia, Lesvos and Thasos), where the prevalence of brucellosis in sheep and goat herds is high, the programme consists of mass vaccination of young and adult female animals (>3months old) and of young male animals (3-6 months old). Moreover, all unvaccinated male animals (>6months old) must be blood-tested annually. In case the male animals were vaccinated during the age of 3-6 months old, they undergo blood-sampling after a minimum period of 12 months. In the islands, where the disease is in low prevalence among sheep and goat herds test and slaughter policy is indicated. All animals over 6 months old must be blood-tested and the positive ones are slaughtered within a month.

2. Measures in place The restrictive measures foreseen for brucella positive herds of sheep and goats are the following: 1) Infected herds must remain in isolation. Thus, the exit and introduction of animals is prohibited, except for the cases where the animals are going to be slaughtered, under the supervision of the local veterinary authority. These animals must be accompanied by the appropriate documents. 1a) In the Eradication Zone (EZ) even if only one of the animals in an M4 holding is found to be positive, all the other animals are tested within 10 working days from the announcement of the lab results. Positive animals are slaughtered, and these tests are repeated every 2-4 months, until all the samples tested are negative. The status of the holding then becomes M2 and the isolation measures are lifted. 1b) In the Vaccination Zone (VZ), positive animals are isolated until their slaughter. When applicable, blood samples are taken from i) all female unvaccinated animals ii) all adult female animals that were vaccinated at an age of 3-6 months provided at least 12 months have elapsed from their vaccination date. Negative unvaccinated animals are vaccinated as soon as possible. The isolation measures are lifted when all positive animals have been slaughtered, all negative unvaccinated animals have been vaccinated and the cleaning and disinfection procedures at the holding have been completed. 2) Infected animals are marked with a "Δ" shape punch in the right ear and must be in isolation from the other animals until their slaughter. A special, clearly separated space is defined for the isolation of positive and suspect animals so that they do not come in contact with the other animals of the holding. 3) An epidemiological investigation should be conducted to identify the source of infection as well as the herds linked to the positive herd. These herds are also investigated. The results are registered in the Certificate of the Epidemiological Investigation. 4) Aborted fetuses, placenta, animals that were stillborn die right after their birth are collected and destroyed according to the current legislation concerning the management of animal by- products. The appropriate parts of these are collected as samples (with every necessary precaution) and sent to the National Reference Laboratory for Brucellosis so that the necessary lab tests for the confirmation of the disease can take place. 5) The presence of other species susceptible to Br. melitensis (eg dogs, horses, cattle, horses) is prohibited. If such animals exist, they are serologically tested and the positive are slaughtered or euthanized without compensation. 6) Milk coming from infected animals cannot be used for human consumption. It must be collected in separate vessels and can be used only under the procedures of Regulation (EC) No 1069/2009. 7) Milk coming from non infected animals of the holding can be used according to Regulation (EC) No 853/2004 Annex III Section IX Chapter I Paragraph I.3.(a). 8) Manure and bedding must be collected in a special place and sprayed everyday with the relevant disinfectant unless it will be covered with soil. It is forbidden to transport these

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materials outside the holding. In case these are moved, they are characterized as category 2 materials and are treated according to the procedures of Regulation (EC) No 1069/2009. 9) Materials that have come to contact with abortion or delivery fluids, placentas or foetuses are disinfected by coiling or spraying or dipping in the suitable disinfectant. 10) All holdings in a radius of 500m from the infected one are checked in high priority if they have not been already checked in the framework of the programme. 11) In case the positive holding utilizes a pasture no other holding is permitted to move to it until 60 days have passed since the departure of the positive holding from that pasture. 12) The slaughter of infected animals must be carried out according to Regulation (EU) 2017/625 within 30 days from the announcement of the positive test results. Transport of these animals to the slaughterhouse is carried out according to the regulations in effect and after all necessary measures to contain the infection have been taken. 13) The carcasses of infected animals undergo a detailed inspection. All parts and organs that according to the regulations in effect are to be confiscated are confiscated. Samples are also taken and sent to the corresponding Veterinary Lab. 14) 1-7 days after the last infected animal has been culled, cleaning and disinfection of the premises and the equipment of the holding takes place. At most 15 days after the culling of the last positive animal ant at least 7 days after the completion of the first disinfection, the procedure is repeated. Cleaning and disinfection are carried out under vet supervision and a certificate of disinfection is signed in two copies. One copy is given to the farmer and the other is kept in the archive of the LVA. 15) The importance of these zoonoses for his holding, for the public health and for the health of anyone involved is pointed out to the farmer. Compliance to the aforementioned measures is a necessary prerequisite for the successful implementation of the programme as well as for the compensation of the farmers, who should be notified in writing about the measures of biosecurity they should apply in their holding.

3. Notification system in place to the national competent authority Brucellosis is a notifiable disease. Any suspected or confirmed case of the disease (both in humans or animals) must be reported to the relevant authorities. The notification of human cases of brucellosis is made by the Hellenic National Public Health Organisation (EODY) of the Hellenic Ministry of Health to the Department of Zoonoses. The number of human cases recorded by EODY during 2010-2018 are: 96 human cases in 2010, 105 in 2011,123 in 2012,159 in 2013,135 in 2014, 110 in 2015,122 in 2016, 92 in 2017 and 97 in 2018.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection EPIDEMIOLOGICAL SITUATION IN THE MAINLAND (Vaccination Zone). DATA ANALYSIS Summary results of the official mass vaccination 2019 programme: Mass vaccination carried out in the Mainland. During 2019, based on vaccination records and reports from the Veterinary Services of the Regional Units, 587.996 sheep and goats from 19.885 herds were vaccinated with the vaccine REV-1. EPIDEMIOLOGICAL SITUATION IN THE ISLANDS (Eradication Zone). DATA ANALYSIS In the islands [eradication zone, (except the islands of Evia, Lesvos and Thasos)], the 2019 herd prevalence and incidence rates among tested sheep and goat herds were reported 3.34% and 0.81 % respectively. What is important is that there is an increase in both prevalence and incidence in comparison to 2018, when the herd prevalence was 1.99% and the herd incidence 0.56%. The islands of Evia, Lesvos and Thasos have been excluded from the eradication policy and belong to the mainland vaccination programme status. Summary results of the zoonoses monitoring in the year 2019 from the eradication zone: Number of herds under the programme (official control): 22188, Number of animals under the programme (official control): 5.770.543, Number of herds tested: 1.109, Number of herds positive: 37, Number of new flocks positive: 9, Number of animals tested individually: 198.555, Number of animals positive: 63, Total number of animals slaughtered: 61. Epidemiological and Technical evaluation. The Sheep and Goat Brucellosis control and eradication programme has been implemented in the mainland and islands of Greece in 2019. The 2019 B. melitensis programme was carried out with co-financing status by the EU based on the Grant Decision of the European Commission approving national programmes Greece 17

and associated funding (Decision Number SANTE/VP/2019/EL/SI2.798233).

19. General evaluation: Mycobacterium tuberculosis complex

1. History of the disease and/or infection in the country

The Greek programme started in 1977 with the implementation of tuberculin skin test to all bovine animals over 6 weeks old of age.

2. Evaluation of status, trends and relevance as a source for humans Greece is not an officially tuberculosis free country and there are no officially tuberculosis free regions. Tuberculosis is a notifiable disease. Any suspected or confirmed case of the disease (both in humans or animals) must be reported to the relevant competent authorities. There are no data reported from the Hellenic Public Health Organisation (EODY) regarding the Mycobacterium bovis situation in humans.

3. Any recent specific action in the Member State or suggested for the European Union The national programme for the eradication of Bovine Tuberculosis is entirely financed by the Greek state.

20. Description of Monitoring/Surveillance/Control programmes system: Cattle (bovine animals). Mycobacterium tuberculosis complex 1. Monitoring/Surveillance/Control programmes system A Bovine Tuberculosis eradication programme is implemented in the whole territory of Greece.

2. Measures in place The measures foreseen for herds infected by tuberculosis are the following: 1) Infected herds must remain in isolation. Thus, the exit and introduction of animals is prohibited, with the exception of animals that are to be slaughtered. These animals must move to the slaughterhouse accompanied with the necessary documents. 2) Infected animals get marked with a "T" shape punch in the right ear and are placed in isolation from all other animals until their slaughter. A special, clearly separated space is defined for the isolation of positive and suspect animals so that they do not come in contact with the other animals of the holding. 3) An epidemiological investigation must be conducted to define the source of infection. The herds epidemiologically linked to the outbreak are also determined and investigated. The results are registered in the Certificate of Epidemiological Investigation. 4) Aborted fetuses, placenta and animals that were stillborn or died right after their birth are collected, sampled and destroyed, according to the current legislation concerning the management of animal by-products, taking all necessary precautions. The samples are sent for confirmatory testing to the competent laboratory/ies. 5) The milk of infected or suspect animals can not be used for human consumption. It must be collected in separate vessels and either be destroyed according to the procedures of Regulation (EC) No 1069/2009 or be used as feed in the holding after heat treatment. 6) The milk of non-infected animals of the holding can be used according to Regulation (EC) Greece 18

No 853/2004 Annex III Section IX Chapter I Paragraph I.3.(a). 7) Manure and bedding must be collected in a special place and sprayed everyday with the appropriate disinfectant, unless it is covered with soil. It is forbidden to transport these materials outside the holding for a minimum of three (-3-) weeks after the application of the disinfectant. When they are moved, they are characterized as "category 2" materials and are treated according to the procedures of Regulation (EC) No 1069/2009. 8) Materials that have come to contact with abortion or delivery fluids, placentas or fetuses are disinfected by coiling or spraying or dipping in the suitable disinfectant. 9) All holdings in a radius of 500m from the infected one are examined in high priority, unless they have already been examined in the framework of the tuberculosis programme. 10) In case the positive holding utilizes a pasture no other holding is permitted to move to it until 60 days have passed since the departure of the positive holding from that pasture. 11) The slaughter of infected animals must be carried out according to Regulation (EU) 2017/625 within 30 days from the announcement of the positive test results. Transport of these animals to the slaughterhouse is carried out according to the Regulations in effect and after all necessary measures to contain the infection have been taken. 12) The carcasses of infected animals undergo a detailed inspection. Parts and organs are confiscated in accordance to the Regulations in effect. Samples are also taken and sent to the corresponding Veterinary Lab. 13) 1-7 days after the last infected animal has been culled, cleaning and disinfection of the premises and the equipment of the holding takes place. At most 15 days after the culling of the last positive animal and at least 7 days after the completion of the first disinfection, the procedure is repeated. Cleaning and disinfection are carried out under veterinary supervision and a certificate of disinfection is signed in two copies. One copy is given to the farmer and the other is kept in the archive of the LVA. 14) The importance of tuberculosis for Animal Health and the affected holding in particular, for Public Health, but also for the health of all animal handlers individually is stressed out to the farmer. Compliance to the aforementioned measures is a necessary prerequisite for the successful implementation of the programme as well as for the compensation of the farmers, who should be notified in writing about the measures of biosecurity they should apply in their holding.

3. Notification system in place to the national competent authority Tuberculosis is a notifiable disease. Any suspected or confirmed case of the disease (both in humans or animals) must be reported to the relevant authorities. The notification of human tuberculosis cases is made by the Hellenic National Public Health Organisation (EODY) of the Hellenic Ministry of Health to the Department of Zoonoses.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection In 2019, the herd prevalence and incidence rates among tested holdings were reported 2.58% and 1,76% respectively. Summary results of the zoonoses monitoring in the year 2019: Number of holdings under the programme: 18.003, Number of animals under the programme: 805.233, Number of holdings tested: 3.741, Number of holdings positive: 93, Number of new herds positive: 45, Number of animals tested individually: 149.871, Number of animals positive: 2.294, Total number of animals slaughtered in the framework of the tuberculosis programme: 2.871.

5. Additional information In 2017, two bovine herds of a combined number of 220 animals that were sharing the same stable in the area of Preveza were tested with the intrademical test as part of the eradication programme framework. Of the 220 animals tested, 127 were found to be positive. In line with the programme's directives, all 127 animals were slaughtered. During the testing process the owner of the herd and his son also tested positive by the tuberculin intradermal test. The Department of Zoonoses of the Ministry of Rural Development and Food therefore decided that a more thorough examination of the case should take place. Consequently, samples taken from the infected animals at the slaughterhouse were sent to: a) the Office of Biological Product Diagnostic Pathology and Parasitology Laboratory of Greece 19

Thessaloniki for histological examination – two (-2-) samples, both diagnosed as positive in tuberculosis and b) the EURL in the Complutense University of Madrid (Universidad Complutense Madrid) for bacteriological culture and identification with molecular techniques – eighteen (-18-) samples, four (-4-) of which gave a positive culture and three (-3-) were found positive by a molecular examination (RT-PCR). The DVR-spoligotyping technique that was performed on the same samples by the EURL identified the Mycobacterium caprae species and especially the SB2484 stain which was found for the first time.

21. General evaluation: Salmonella spp

1. History of the disease and/or infection in the country The legal provisions in place and relevant requirements (Zoonoses Directive 2003/99 and Zoonoses Regulation 2160/2003) had significantly improved the effectiveness of the existing monitoring situation and management practices in the field of Salmonella surveillance. Rapid adaptation and compliance with the mandatory EU regulations for the control of zoonotic salmonella in poultry populations helped to improve the country's epidemiological situation regarding salmonellosis in both humans and poultry.

2. Evaluation of status, trends and relevance as a source for humans Due to the implementation of the National Salmonella Control Programmes in flocks of Gallus gallus and Turkeys, there was a reduction in human cases until 2015, when the number of human cases showed again an increase. Information on human salmonellosis is reported by EODY (Hellenic National Public Health Organisation).

https://eody.gov.gr/wp-content/uploads/2020/04/Epidemiological-data-for-Salmonellosis_2004-2019.pdf

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22. Description of Monitoring/Surveillance/Control programmes system: Gallus gallus (fowl) and Turkeys-Salmonella spp 1. Monitoring/Surveillance/Control programmes system The National Salmonella Control Programmes (NSCP's) are implemented in the whole territory of Greece and comply with the requirements laid down in Regulations: Reg. (E No 2160/2003, Reg. (E No 200/2010, Reg. (E No 517/2011, Reg. (E No 200/2012, Reg. (E No 1190/2012, Reg. (E No 1177/2006, Reg. (E No 1237/2007 and Reg. (E 2073/2005. The bodies responsible for implementing the NSCP's are: 1. The Department of Zoonoses of the Animal Health Directorate / Sustainable Animal Production and Veterinary Services Directorate General) /Ministry of Rural development and Food. 2. The regional veterinary authorities. 3. The Thessaloniki Centre of Veterinary Institutes (Ministry of Rural development and Food) and the Ioannina Veterinary Laboratory (Ministry of Rural development and Food). 4. The Halkida Veterinary Laboratory, which is also the National Reference Laboratory for Salmonella (NRL). All official samples collected as part of the NSCPs are examined at the three above mentioned accredited laboratories. 5. The operators (FBOs) of the Turkey and Gallus gallus poultry farms. 6. The private laboratories carrying out analyses on own-check samples as part of the NSCP's. In order the NSCP's to be applied in a uniform way across the country, the CCA has published a number of ministerial decisions and circulars and has created manuals with detailed instructions related to the programmes’ procedures. All the national decisions and some of the relevant guides can be found at: http://www.minagric.gr/index.php/el/for-farmer-2/animal-production/ptina/208- astheneiesptinon-cat/459-salmonelosi

2. Measures in place

Measures in case of positive findings: According to the current EU Directives and Community Legislation and in particular according to the requirements laid down under part C, D and E of Annex II of Reg. (E No 2160/2003. The Key points of the NSCP’s in order to achieve their objectives (reduction of the relevant Salmonella serotypes at least to the level set by the EU regulations in the relevant poultry populations) are: 1. the implementation of controls (official and own-check controls during which official and sampling at the initiative of the FBOs is carried out following at least the minimum sampling requirements described under the relevant EU Regulations 2.the implementation of measures (restrictive or other measures are placed on the infected flocks and on their products) in case of suspect (detection of Salmonella spp) and positive results (detection of the targeted Salmonella serovars) 3.the implementation of vaccination (obligatory in flocks of laying hens Gallus gallus and voluntary in flocks of breeding hens Gallus gallus) 4.the Implementation of strict biosecurity measures Biosecurity is part of the national salmonella control programmes (annex attached to the national decisions for the implementation of NSCPs). Guides are in place and their implementation is linked to compensation. In addition, they are regularly assessed by the competent authority (CA). The CA assesses the biosecurity measures each time they perform official control for the NSCP. This assessment is followed by a written report which also includes the results from the biosecurity assessment 5. the existence of a mandatory notification system and of a system to monitor the implementation of the NSCPs at central and at regional level: Each case of positive Salmonella spp result is notified without delay to the regional veterinary CA in order to take measures in case of suspicion and to the Department of Zoonoses. In addition, every six months, the state veterinary laboratories report the results of officials Greece 21

checks performed in the context of NSCP to the Department of Zoonoses and the approved private laboratories report the results of the own-check received in the context of NSCP to the NRL, which then report them to the Department of Zoonoses. In addition, all the Regional Veterinary Units report twice per year to the Department of Zoonoses detailed information for all the relevant poultry flocks in their territory. This information includes data on the official and own -check controls (dates, results, type of sampling), data on vaccination (number of vaccine doses administered), number of infected birds culled or destroyed, etc At regional level, the monitoring of the implementation of the programme is achieved by the following way: Each flock is identified with a unique code number consisting of the following elements: code number of the holding/number of the house/date of placement of the flock in the house. During official control official veterinarian records all the information regarding the flock (date of placement, own-check results, data on vaccination et. Currently, Department of Zoonoses in collaboration with the Directorate of E- Government of the Ministry, have developed an on-line system for the on- line submission of all the information related to sampling and laboratory testing. This system is not still fully operational. 6. The existence of a system for the compensation of the owners for the value of their birds slaughtered or culled and the eggs destroyed, or heat treated: Owners of breeding hens, laying hens and breeding turkeys who are obliged to slaughter or destroy contaminated flocks or destroy contaminated eggs within the framework of NSCPs are compensated in accordance with the relevant joint ministerial decision on financial aid. The amount of the compensation is calculated on the basis of value tables drawn up by the sector and validated by the Ministry of Finance. The valuation/valorisation of birds is defined at central level annually by the issue of an annual Joint Ministerial Decision (Ministry of Finance and Ministry of Rural Development and Food), which is publicly available at official Gazette’s web site and Ministry’s web site. In the above mentioned Joint Ministerial Decision there is a scale of compensation of birds. There is a link between compensation and effective biosecurity measures: FBO’s that do not comply with the minimum requirements on biosecurity, as set out in the national legislation for the implementation of NSCP, do not take any compensation in case of a positive flock although they are obliged to implement the measures of part C , D and E of Annex II of Reg. (E No 2160/2003. The conditions that must be met by the farmer, the procedure of payment and all required supporting documents are described in detail in the above JMD.

3. Notification system in place to the national competent authority Salmonellosis is a notifiable disease in Greece in both human and animals.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection For the year 2019 the EU targets were achieved in breeding, laying and broiler hens Gallus gallus as well as in breeding and fattening turkeys, similarly to 2018. In 2017, the target had been achieved in all the above-mentioned poultry species except from breeding hens Gallus gallus, for which the EU target had not been achieved (prevalence of the targeted Salmonella serovars was 1.8%). The results of the implementation of the NSCPs can be found in the EFSA annual summary reports. In the following figures one can notice the trend for the Salmonella targeted (red colour) and Salmonella spp. (blue colour) serovars in flocks of breeding, laying and broiler flocks Gallus gallus and in flocks of fattening turkeys (source of information is Dept. of Zoonoses of the Animal Health Directorate of the Hellenic Ministry of Rural Development and Food):

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Fig.1 Prevalence of targeted Salmonella serovars and Salmonella spp. serovars in breeding hens Gallus gallus, 2007-2019, Greece.

Fig.2 Prevalence of targeted Salmonella serovars and Salmonella spp. serovars in laying hens Gallus gallus, 2008-2019, Greece.

Fig.3 Prevalence of targeted Salmonella serovars and Salmonella spp. serovars in broiler hens Gallus gallus, 2009-2019, Greece.

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Fig.4 Prevalence of targeted Salmonella serovars and Salmonella spp. serovars in fattening turkeys, 2010-2019, Greece.

23. Description of Monitoring/Surveillance/Control programmes system: All foodstuffs-Salmonella spp 1. Monitoring/Surveillance/Control programmes system Contaminated Food Surveillance system: Routine examination and selective official sampling at retail level, processing plan and slaughterhouse carried out based on National and Community legislation. Method used: the recommended method is ISO 6579:2002/2017 in accordance with Regulations (E No 2073/2005, No1441/2007, No 1086/2011, No 209/2013 and No 217/2014 on microbiological criteria for foodstuffs.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection The results of the national surveillance can be found at EFSA tables.

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24. General evaluation: Brucella abortus

1. History of the disease and/or infection in the country The Greek programme started in 1977 and included blood sampling of all bovine animals over 12 months old. In 2000, it was decided to apply REV-1 vaccinations in all female semi-wild bovines (over 4 months old) simultaneously with the blood sampling of all male bovines over 12 months old in specific mountainous areas. Vaccination is applied once in their life, while blood-sampling is implemented annually. In 2003, the RB-51 vaccine was introduced in dairy farms of the Regional Unit (RU) of Thessaloniki. Since 2013 the same vaccine has been implemented in dairy farms of the RUs of Pella and Larisa. In late November 2019 the area of its use has been extended to the RU of Pieria.

2. Evaluation of status, trends and relevance as a source for humans

Greece is not an officially brucellosis free country and there are no officially brucellosis free regions. Brucellosis is a notifiable disease. Any suspected or confirmed case of the disease (both in humans or animals) must be reported to the relevant authorities. The disease in humans is mainly attributed to Br. melitensis. The presence of Br. abortus in animals compared to Br. melitensis in sheep & goat, has a minor public health impact in humans based on the epidemiology and official records from the public health services, however there are no detailed data on the pathogenic agent of the reported human cases.

3. Any recent specific action in the Member State or suggested for the European Union The Bovine Brucellosis Programme is entirely financed by the state budget.

4. Additional information The REV-1 Vaccination Programme in semi-wild bovines in specific mountainous areas of the country was decided because Br. melitensis strains were isolated from aborted fetuses of these animals. Moreover, semi-wild bovines commonly share the same pastures with sheep and goats. The RB-51 Vaccination programme in the Regional Units (RUs) of Thessaloniki, Pella, Pieria and Larisa is an additional preventive measure, aiming to the rapid reduction of the prevalence of BB. The policy of using the RB-51 vaccine (Brucella abortus strain) was initially implemented in a specific high-risk area (Thessaloniki) in order to facilitate the progress of the existing Brucellosis eradication programme in Bovine Herds (dairy herds). Since 2013, this vaccination programme was expanded to the RUs of Pella and Larisa, and since late 2019 to the RU of Peria. The vaccination programme with the RB-51 vaccine is implemented concurrently with the eradication programme.

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25. Description of Monitoring/Surveillance/Control programmes system: Cattle (bovine animals)- Brucella abortus 1. Monitoring/Surveillance/Control programmes system A Bovine Brucellosis eradication programme is implemented in whole Greece, except specific mountainous areas where a control programme based on REV-1 vaccinations is in force.

2. Measures in place The measures foreseen in infected with brucellosis herds are the following: 1) Infected herds must remain in isolation. Thus, the exit and introduction of animals is prohibited, with the exception of animals that are to be slaughtered. These animals must move to the slaughterhouse accompanied by the necessary documents. 2) Infected animals are marked with a "Δ" shape punch in the right ear and are placed in isolation from all other animals until their slaughter. A special, clearly separated space is defined for the isolation of positive and suspect animals so that they do not come in contact with the other animals of the holding. 3) An epidemiological investigation must be conducted to define the source of infection. The herds epidemiologically linked to the outbreak are also determined and investigated. The results are registered in the Certificate of Epidemiological Investigation. 4) Aborted fetuses, placenta and animals that were stillborn or died right after their birth are collected, sampled and destroyed, according to the current legislation concerning the management of animal by-products, taking all necessary precautions. The samples are sent for confirmatory testing to the competent laboratory/ies. 5) The presence of other species susceptible to Br. abortus (e.g. dogs, horses, cattle, horses) is prohibited. If such animals exist, they are serologically tested and the positive are slaughtered or euthanized without compensation. 6) Milk coming from infected animals cannot be used for human consumption. It must be collected in separate vessels and can be used only under the procedures of Regulation (EC) No 1069/2009. 7) Milk coming from non infected animals of the holding can be used according to Regulation (EC) No 853/2004 Annex III Section IX Chapter I Paragraph I.3.(a). 8) Manure and bedding must be collected in a special place and sprayed daily with the appropriate disinfectant unless it is covered with soil. It is forbidden to transport these materials outside the holding. If they are moved, they are characterized as "category 2" materials and are treated according to the procedures of Regulation (EC) No 1069/2009. 9) Materials that have come to contact with abortion or delivery fluids, placentas or fetuses are disinfected by coiling or spraying or dipping in the suitable disinfectant. 10) All holdings in a radius of 500m from the infected one are examined in priority unless they have already been examined in the framework of the tuberculosis programme. 11) In case the positive holding uses a pasture no other holding is permitted to move to it until 60 days have passed since the departure of the positive holding from that pasture. 12) The slaughter of infected animals must be carried out according to Regulation (EU) 2017/625 within 30 days from the announcement of the positive test results. Transport of these animals to the slaughterhouse is carried out according to the Regulations in effect and after all necessary measures to contain the infection have been taken. 13) The carcasses of infected animals undergo a thorough inspection. Parts and organs are confiscated in accordance to the Regulations in effect. Samples are also taken and sent to the corresponding Veterinary Lab. 14) 1-7 days after the last infected animal has been culled, cleaning and disinfection of the premises and the equipment of the holding takes place. At most 15 days after the culling of the last positive animal and at least 7 days after the completion of the first disinfection, the procedure is repeated. Cleaning and disinfection are carried out under vet supervision and a certificate of disinfection is signed in two copies. One copy is given to the farmer and the other is kept in the archive of the LVA. 15) Farmer is informed about the importance of this zoonoses regarding both the health of the animals of the infected holding, as well as public health and the health of all persons handling animals. Compliance to the measures is a prerequisite for the successful implementation of Greece 26

the programme as well as for the compensation of the farmers, who should be notified in writing about the measures of biosecurity they should apply in their holding.

3. Notification system in place to the national competent authority Brucellosis is a notifiable disease. Any suspected or confirmed case of the disease (both in humans or animals) must be reported to the relevant authorities. The notification of human cases of brucellosis is made by the Hellenic Public Health Organisation (EODY) of the Hellenic Ministry of Health to the Department of Zoonoses. According to the available recorded data of EODY, 96 human cases were recorded in 2010, 105 in 2011,123 in 2012,159 in 2013,135 in 2014,110 in 2015,122 in 2016, 92 in 2017, and 97 in 2018.

4. Results of investigations and national evaluation of the situation, the trends and sources of infection In 2019, the herd prevalence and incidence rates among tested herds were reported 2,86% and 1,27% respectively. Summary of results of the monitoring plan in the eradication zone for the year 2019: Number of herds under the programme: 10.726, Number of animals under the programme: 330.035, Number of herds tested: 3.420, Number of positive herds: 85, Number of new herds found positive: 28, Number of animals tested individually: 41.504, Number of animals positive: 1.296, Total number of animals slaughtered: 1.206.

26. Food-borne Outbreaks

1. System in place for identification, epidemiological investigations and reporting of food-borne outbreaks Foodborne outbreaks are reported via the Mandatory Notification System (MNS) of the Hellenic National Public Health Organization (EODY). Once a food-borne outbreak is notified, the public health professionals conduct an epidemiological investigation in order to estimate the extent of the outbreak, identify the source and implement control measures. At the same time, the Public Health Directorate of the competent Prefecture, the National Food Agency, as well as the Ministry of Rural Development and Food are informed, and appropriate action is taken.

2. Description of the types of outbreaks covered by the reporting Outbreaks caused by consumption of drinking water are also included in the reporting system. Moreover, both general and household outbreaks are reported via the MNS. Finally, regarding the causative agents the MNS includes outbreaks caused by bacteria, viruses, parasites, funguses and their products.

3. National evaluation of the reported outbreaks in the country The number of reported food-borne outbreaks has been quite stable since 2004. However, data of the MNS should be interpreted with caution due to the probable under-reporting. It should be mentioned that the reporting date was used for data analysis. Regarding the severity of illness, 39 (4.8%) of the 806 outbreak-related cases, were hospitalised and no death was recorded in 2019.

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27. Institutions and laboratories involved in antimicrobial resistance monitoring and reporting 1) Three state veterinary laboratories were involved in testing of the samples  The National Reference Laboratory for Salmonella & AMR of Chalkida was responsible for sample testing (isolation/identification/storage), susceptibility testing of the isolates and reporting of the data to the competent authority.  National Veterinary center of Athens/ Department of Food Hygiene was responsible for meat sample testing (isolation/identification/storage).  National Veterinary center of Thessaloniki/ Department of Food Hygiene was responsible for meat sample testing (isolation/identification/storage).  National Veterinary center of Thessaloniki/ Veterinary Laboratory of Ioannina was responsible for ceacal sample testing (isolation/identification/storage)

2) Department of Zoonoses of the Animal Health Directorate of Ministry of Rural Development and Food (MRDF )was responsible for the reporting of AMR according to Directive 2003/99/EC. 3) Department of slaughterhouses of the Veterinary Public Health Directorate of the MRDF was responsible for the implementation and supervision of the AMR monitoring programme. 4) Veterinary services located in 18 Regional Units of the country were responsible for sampling in Slaughterhouses and Veterinary services located in 8 Regional Units for sampling at retail.

28. General Antimicrobial Resistance Evaluation

1. Situation and epidemiological evolution (trends and sources) regarding AMR to critically important antimicrobials (CIAs) over time until recent situation In general the situation of the AMR trends in Greece is as follows :  The percentage of fully sensitive to antibiotics commensal E.coli ,in fattening pigs is low (8,2 % in 2017 & 14,4% in 2019)  There is an increase in the resistance to colistin (0,6% in 2017 to 3,4 in 2019)

2. Public health relevance of the findings on food-borne AMR in animals and foodstuffs AMR in bacteria from food animals and food are considered a constant threat to public health

3. Recent actions taken to control AMR in food producing animals and food National Action Plan for AMR was developed in Greece, in 2019, compromising three Ministries (Health, Agriculture and Environment).

29. General Description of Antimicrobial Resistance Monitoring; Caecal sample from fattening pigs / Indicator commensal E. Coli

1. General description of sampling design and strategy(a) The pig population in Greece is declining . The most intensive farming holdings are found at the following regions according to the population of sows.

1ST DYTIKI ELLADA & IPEIROS

2ND THESSALIA

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3RD STEREA ELLADA

4TH MACEDONIA

5TH PELOPONNISOS

General description of sampling design and strategy in slaughterhouses. Pig’s caecal samples / commensal E. Coli :  18 Regional Units of the country were responsible for sampling in slaughterhouses.  The samples were randomly collected at slaughter from 26 slaughterhouses with large throughput by official veterinarians/  One pig’s caecum was collected from each epidemiological unit.

According to the Decision EU 652/2013 and the Ministerial Decision no 1582/271253 /24-10- 2019 OJ B 3986, 153 samples were collected by random sampling and tested. Of the 153 samples 146 isolates of commensal E. coli were isolated and tested for antimicrobial sensitivity using the MIC method (Minimum Inhibition Concentration)

The random sampling plan has been stratified per slaughterhouse by allocating the number of samples from domestically produced animals collected per slaughterhouse, proportionally to the annual throughput of the slaughterhouse .

According to the article 4 of Ministerial Decision (sampling plan) the sampling was performed at the slaughterhouses of the Regional Units of :  Evoia, Karditsa, Argolida, Ileia, Ioannina, Drama, Kilkis, Pieria, Imathia, Trikala, Pella, Preveza, Magnisia, Fthiotida, Xanthi, Kozani, Kastoria, Korinthia.

Picture 1 shows the distribution of slaughterhouses on the map of Greece.

The 18 regional units selected for the collection of samples and the respective slaughterhouses in the area are marked with a red circle. The regional units involved were limited to the mainland due to the fact that the most intensive farming, holdings are located there. Specifically, the highest populations of extensive farming pigs are found in the regions of Ipeiros (Ioannina, Preveza), Thessalia (Larissa, Trikala, Karditsa), Peloponnhsos (Korinthos ,Lakonia) & Sterea Ellada (Evoia). In the islands there are holdings of small numbers of animals which are slaughtered during the Christmas period.

Procedure of selection of isolates for susceptibility testing : - One isolate per bacterial species, per epidemiological unit was tested and reported to EFSA, according decision 2013/652/EU.

Method used for collecting data : The NRL and the competent authority collect the relevant data from the official samplers, veterinary laboratories and the food business operators.  2. Stratification procedure per animal population and food category The selection of the areas for the sampling plan at the slaughterhouse were based on: i) the population of the farms nearby (picture 2) ii) the capacity of the slaughterhouses iii) the proximity to the related Reference Laboratories, (picture 2)

AMR monitoring programme in 2019 was implemented from July to December (three seasons were covered). 3. Randomisation procedure per animal population and food category Animal population sampling was based on a random selection of slaughterhouses with the

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largest throughput in the 18 selected regional units of the country. 4. Analytical method used for detection and confirmation(b) The laboratory methodology followed for the isolation / confirmation and identification of commensal E.coli was in accordance to EURL –AR protocols. All E.coli strains were identified using chromogenic agar TBX and when required, using biochemical tests (API20E, Biomerieux).

5. Laboratory methodology used for detection of antimicrobial resistance(C) As required by Decision 2013/652/EC.

30. General Description of Antimicrobial Resistance Monitoring; Caecal sample from fattening pigs / ESBL-/AmpC- /Carbapenamase- producing E.Coli

1. General description of sampling design and strategy(a) 1) The pig population in Greece is declining . The most intensive farming holdings are found at the following regions according to the population of sows.

1ST DYTIKI ELLADA & IPEIROS

2ND THESSALIA

3RD STEREA ELLADA

4TH MACEDONIA

5TH PELOPONNISOS

General description of sampling design and strategy in slaughterhouses. Pig’s caecal samples / E. Coli producing ESBL/AMPC/Carbapenamases:  18 Regional Units of the country were responsible for sampling in slaughterhouses.  The samples were randomly collected at slaughter from 26 slaughterhouses with large throughput by official veterinarians.  One pig’s caecum was collected from each epidemiological unit.

According to the Decision EU 652/2013 and the Ministerial Decision no 1582/271253 /24-10- 2019 OJ B 3986, 153 samples were collected by random sampling and tested for antimicrobial resistance.

Picture 1 shows the distribution of slaughterhouses on the map of Greece.

Ipeiros (Ioannina, Preveza), Thessalia (Larissa,Trikala, Karditsa), Peloponnhsos (Korinthos, Lakonia) & Sterea Ellada (Evoia). In the islands there are holdings of small numbers of animals which are slaughtered during the Christmas period.

Samples were collected at slaughter from fattening pigs, randomly selected by official veterinarians from 26 slaughterhouses with large throughput. One pig’s caecum was collected from each epidemiological unit. According to the Ministerial Decision 153 samples were finally obtained and tested.  60 E. coli producing ESBL/AmpC have been isolated

Procedure of selection of isolates for susceptibility testing : One isolate per bacterial species, per epidemiological unit was tested and reported to EFSA, according decision 2013/652/EU.

Method used for collecting data : The NRL and the competent authority collect the relevant data from the official samplers, veterinary laboratories and the food business operators. 2. Stratification procedure per animal population and food category The selection of the areas for the sampling plan at the slaughterhouse were based on: i) the population of the farms nearby (picture 2) ii) the capacity of the slaughterhouses iii) the proximity to the related Reference Laboratories, (picture 2)

Pig’s Caecal samples: AMR monitoring programme in 2019 was implemented from July to December (three seasons were covered). 3. Randomisation procedure per animal population and food category Animal population sampling was based on a random selection of slaughterhouses with the largest throughput in the 18 selected regional units of the country. 4. Analytical method used for detection and confirmation(b) The laboratory methodology followed for the isolation of the specific monitoring of the ESBL/AmpC producers and Carbapenamase – producers was in accordance to EURL –AR protocols.

The selective plates used for the monitoring of the ESBL/AmpC producers were in house media (EURL –AR protocol) and for the Carbapenamase – producers the Chromid oxa-48 and Carba plates (Biomerieux) were used.

All E.coli strains were identified using chromogenic agar TBX and when required, using biochemical tests (API20E, Biomerieux).

5. Laboratory methodology used for detection of antimicrobial resistance(C) As required by Decision 2013/652/EC.

31. General Description of Antimicrobial Resistance ; Fresh pig and bovine meat samples / ESBL-/AmpC-/Carbapenamase producing E.coli

1. General description of sampling design and strategy(a) Fresh pig & bovine meat samples : Fresh pig meat/ E.coli producing ESBL/AmpC enzymes from.:  216 samples of fresh pig meat instead of the of the predicted number of 300 that was mentioned at the M.D. were collected. Greece 31

Fresh bovine meat/ E.coli producing ESBL/AmpC enzymes.:  131 samples of fresh bovine meat instead of the predicted number of 150 that was mentioned at the M.D.

One sample of fresh meat was collected per food batch regardless of the country of origin, packaged or in bulk before the expiry date.

Samples were randomly selected at the retail (93 different butcher shops and supermarkets) in eight regional units.

One isolate per bacterial species, per epidemiological unit was tested and reported to EFSA, according decision 2013/652/EU. Method used for collecting data : The NRL and the competent authority collect the relevant data from the official samplers, veterinary laboratories and the food business operators.

2. Stratification procedure per animal population and food category AMR monitoring programme in 2019 was implemented from July to December (three seasons were covered). The two out eight regional units have the cities with the largest populations of people in Greece (Reg. Unit of Thessaloniki and Attica –West &East Attica).

3. Randomisation procedure per animal population and food category The samples of fresh meat were randomly selected from the retail without pre- selection based on the origin of the food. Samples of fresh pig meat and bovine meat were collected at retail from the selected eight regional units in Greece.

4. Analytical method used for detection and confirmation(b) The laboratory methodology followed for the isolation of the specific monitoring of the ESBL/AmpC producers and Carbapenamase – producers was in accordance to EURL –AR protocols. The selective plates used for the monitoring of the ESBL/AmpC producers were in house media (EURL –AR protocol) and for the Carbapenamase – producers the Chromid oxa-48 and Carba plates (Biomerieux) were used. All E.coli strains were identified using chromogenic agar TBX and when required, using biochemical tests (API20E, Biomerieux).

5. Laboratory methodology used for detection of antimicrobial resistance(C) As required by Decision 2013/652/EC.

32. General Description of Antimicrobial Resistance Monitoring; Pig carcasses / Salmonella

1. General description of sampling design and strategy(a) Pig carcases / Salmonella : sampling was in accordance with Regulation (EC No 2073/2005). The isolation of Salmonella strains was performed following ISO 6579-1/2017. One isolate per Salmonella serotype was tested per slaughter batch. Salmonella spp. isolated from, in accordance with Regulation 2073/2005. Pig carcasses samples from slaughterhouses were taken in accordance with Regulation 2073/2005, for the detection of salmonella from carcass surfaces, after cleaning but before chilling, from specific parts of the carcass using sterile sponges that have been moisted with a solvent and from a total surface of 400cm2. Greece 32

According to JOINT MINISTERIAL DECISION No. 1582/271253 / 1 November 2019, 170 strains of Salmonella spp., should have been examined for their susceptibility to antimicrobial substances, which should have been isolated according to Regulation 2073/2005, in the context of official controls and self-controls of companies in slaughterhouses. In 2019, 2 strains of Salmonella spp. from the surface of carcasses of pigs were isolated and serotyped. One Salmonella Typhimurium monophasic was sent by the Veterinary Laboratory of Heraklion to the Veterinary Laboratory of Chalkida and was found to be resistant to Sulfonamides, Trimethoprim, Tetracycline, Chloramphenicol. The second isolate was Salmonella Adabraka and it was fully sensitive to all antibiotics tested. 5. Laboratory methodology used for detection of antimicrobial resistance(C) As required by Decision 2013/652/EC.

Picture 1: Distribution of ungulate slaughterhouses in Greece. Circular markings reflect regional units selected in the sampling plan

Picture 2: Distribution of extensive farming holdings of pigs per region according to the population of sows.

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Picture 3: Caecal sample from fattening pigs / Indicator commensal E. Coli Number of samples obtained per region

REGION REGIONAL UNIT SAMPLES

IPEIROS Preveza 22

Ioannina 2

DYTIKI ELLADA Ileia 14

STEREA ELLADA Fthiotida 4

Evoia 17

KENTRIKI MACEDONIA Imathia 5

Pieria 15

Pella 4

Kilkis 5

ANATOLIKI MACEDONIA Xanthi 4

Drama 15

DITIKI MACEDONIA Kozani 2

Kastoria 2

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PELOPONNISOS Korinthos 10

Argolida 3

THESSALIA Karditsa 10

Magnisia 3

Trikala 16

TOTAL :

8 out of 13 18 153

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