Worms! Parasitic Worms
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Worms! Parasitic Worms! •" Kingdom Animalia: eukaryotic, no cell walls, heterotrophic nutrition, specialized tissues! A. Platyhelminthes! •" Flatworms! –"e.g. planarians ! Platyhelminthes! •" Trematoda = flukes ! –"leaf-shaped! –"parasitic! –"complex life cycles with several larval forms! •" larva = an immature form of an animal; does not look like the adult! •" ______________: where the larva lives! •" ______________: where the adult worm lives! Fluke anatomy! •" _________________ =# having both# functional ovaries and# testes! Clonorchis sinensis (Chinese liver fluke)! Liver fluke# life cycle! Schistosoma:# blood flukes,# male and female (in groove on males body)! ____________________ Schistosoma haematobium! •" portal of entry: skin (Cercariae Larva)! •" source of infection: larvae from fresh water snails! •" disease is not contracted in U.S.A.(we don’t have host snail here), but more than 400,000 immigrants to this country have it ( + 200 million people in Asia, Africa, S. America & the Caribbean)! •" lives primarily in the pelvic veins! •" See Chapter 23 ( page 666-667), figure 23.27! •" Monsters inside me….check it out!!!!: http://animal.discovery.com/videos/monsters- inside-me/! Platyhelminthes •" Cestoda = tapeworms •" hermaphrodi6c = having both ovaries and testes (being both sexes at the same 6me) •" Head is scolex; segments are proglods •" See figure 12.26 –"typical tapeworm: Tapeworm life cycle •" 2 hosts: •" intermediate host: infected by inges6ng tapeworm eggs; contains larval cyst in sKeletal muscle and other organs such as brain (infec6on is called cyscercosis) •" definive host: infected by inges6ng larval cyst; adult tapeworm grows in intes6ne •" examples –" Taenia saginata = beef tapeworm –" Taenia solium = porK tapeworm Tapeworms •" life cycle of porK tapeworm (Taenia solium) •" Endoscopic views of tapeworms in human intes6ne B. Aschelmenthes or Nematoda •" roundworms •" plain, unsegmented worms ranging from microscopic up to about 12 inches •" Ascaris spp. –" ascariasis = intes6nal infec6on –" the largest roundworms: up to 12 inches •" Pg. 736 –" 1/4 of world’s populaon infected (over 1.5 billion people!) –" infec6on by inges6ng worm eggs that can remain in soil 10 years! Ascaris lumbricoides Ascaris in intes6ne Ascaris life cycle •" Trichinella spiralis –"trichinosis = larval cysts in sKeletal muscle –"infecon by ingesng larvl cysts in undercooKed porK or bear meat •" See pg. 737 for life cycle Figure 25.20 Nematoda, cont’d •" Wuchereria bancro;i –"filariasis = worms in lymph vessels –"microfilaria larva transmiYed by Culex spp. mosquitoes –"grow to adults 2—3 inches long –"blocK flow of lymph –"if untreated, aer years of infestaon, leads to elephan6asis (swelling due to accumulaon of fluid in ssues) Filariasis life cycle mosquito ingests microfilaria microfilaria •" mosquito injects microfilaria adults in lymph vessels adult worms blocK lymph vessels elephan6asis Elephan6asis Now it’s 6me for the viruses ! •" ‘virus’ is the lan term for _______ •" “ a piece of bad news wrapped up in protein” •" virology –"the study of viruses A.General nature of viruses •" _____________: have only some of the characteris6cs of life –" no metabolism –" able to reproduce only with considerable help from host cell –" No ribosomes! –" No plasma membrane •" obligate intracellular parasites: can reproduce only inside of living host cells –" will not grow on ar6ficial media (agar, etc) –" do exist outside of host cells; e.g. some are transmiYed through the air •" high mutaon rate •" Viruses and Bacteria compared –" see table 13.1 B. Size of viruses •" SMALL: 20-1,000 nm (1nm = 1/1000 µm) human cell nucleus bacterium with virus inside Fig. 13.1 C. Structure •" NOT _____: much less complex –"neither procaryo6c or eucaryo6c •" individual units called virions or par6cles –"“virion” is to virus as “cell” is to a unicellular organism •" Every virus has a core of nucleic acid (genes) –"either DNA or RNA, never both –"either nucleic acid may be single or double stranded –"called the genome •" Every virus has a coat of protein (the ______) around the nucleic acid –"the capsid protects the genome –"the capsid gives shape to the virus •" Generally, the capsid is subdivided into individual protein subunits called capsomeres •" Some viruses have an outer _________ of fat, protein and carbohydrates –" derived from cell membrane of host cell –" some envelopes may have spiKes (carbo-protein molecules with viral specific components) in order to aach virus to host cells •" see figures 13.2, 13.3, 13.4, 13.5 •" virus without envelope •" ( a nonenveloped virion ) enveloped virus D.Host and 6ssue specificity •" most viruses are _________ (infect only one or a few species of hosts) •" most viruses are 6ssue specific (infect only one Kind of host 6ssue) •" ____________: the species that a pathogen can infect E.Viral replicaon (reproduc6on) and how viruses cause disease •" 5 steps (could view as vulnerabili6es for control) •" 1. _____________: to host cell virus penetrang host cell •" 2. Entry or Penetra.on: into host cell –"either whole virus or just nucleic acid (protein and envelope may be le] behind) –"either into cytoplasm or nucleus of host cell viral replicaon, cont’d •" 3. ____________________: –" A. replica.on of viral nucleic acid (may dissolve host genes to get ingredients) –" B. synthesis of viral protein: viral genes take control of host ribosomes and direct synthesis of viral protein •" 4. ________________: assembly of new virions — up to several hundred •" 5. ____________ of new virions –" enveloped viruses escape one-by-one, taking along some cell membrane for their envelope (a budding process)…host cell may survive –" other viruses may rupture host cell to escape •" About 3,000 to 4,000 virions are released from a single cell infected with poxviruses, whereas a poliovirus-infected cell can release over 100,000 virions! Release by budding The Bacteriophages •" viruses that infect bacteria •" can wipe out a bacterial culture •" some6mes just called “phage” •" the easiest viruses to grow –" See figure 13.6…plaques •" subject of much research –" They o]en make the bacteria they infect more pathogenic for humans! bacteriophage Cell lysis vs. lysogeny •" in the replicaon cycles for bacteriophages and animal viruses, the infec6on may not result in cell lysis –" virus incorporates its DNA or its RNA (via DNA) into a chromosome of the host cell –" virus is propagated each 6me the cell’s chromosome is reproduced •" lysogeny= the conditon in which viruses and bacteria coexist without damage to each other •" See figure 13.12 (next slide) Lyc Cycle vs. Lysogenic Cycle (fig 13.12) More on lysogeny •" Host’s DNA is not destroyed & viral genome remains inac6ve in the cell •" a “hibernang” virus for generaons…then excised later to a ly6c virus? •" Many bacteria that infect humans are lysogenized by phages! –" Some phage genes in the bacterial chromosome cause produc6on of toxins or enzymes that cause pathology in humans! (e.g. the diphtheria toxin is a bacteriophage product; C. diphtheriae without the phage are harmless! ) F. Classificaon of viruses •" Based on type of nucleic acid, strategy for replicaon, and morphology •" Virus family names end in -viridae •" Genus names end in -virus •" A viral species shares the same gene6c informaon and niche •" Example: Family Herpesviridae,genus Simplexvirus, human herpesvirus 2 •" See table 13.2 for reference of the families of viruses that affect humans A closer looK at one RNA virus: a retrovirus •" Retroviridae, genus: Lenvirus, HIV •" retroviruses carry their own enzyme, called__________________ •" this enzyme uses viral RNA to synthesize DNA (reversal of the usual biochemical direc6on) in the host cell •" this newly synthesized viral DNA integrates into a host cell’s chromosome as a provirus ( see figure 13.19 ) •" HIV is an example _____ •" name of virus: human immunodeficiency virus •" common name: AIDS virus –"But AIDS denotes only the final stage of a long infecon •" nucleic acid: ss-RNA w-envelope, 2 iden6cal strands of RNA –"a retrovirus –"once in host cell, changes to DNA and is incorporated into host chromosome •" HIV’s RNA becomes DNA and enters host chromo- some HIV •" related viruses: most mammals have similar viruses •" dis6nguishing features: unusual spiKes (______), reverse transcriptase HIV infec6ng a T cell HIV budding from infected host cell •" Fig. 13.19 Figure 19.14 Another RNA virus worth no6ng… •" The Influenza Virus •" Figure 24.15 •" www.flu.gov Another interes6ng ‘family’ of viruses are the Herpesviridae •" DNA viruses, nearly 100 herpesviruses known •" important diseases in this group include –"Human herpes Simplexvirus •" type I: cold sores (fever blisters) HHV - 1 •" type II: genital herpes HHV - 2 –"ChicKenpox: HHV - 3 (Varicellavirus) –"infec6ous mononucleosis: HHV - 4 –"Cytomegalovirus: HHV-5 –"Kaposi’s sarcoma: HHV-8 –"Others, too see pg. 404 •" classic examples of ________ viral infec6ons Latent infec6on •" some viruses enter host cell and remain dormant or replicate slowly with liYle damage to host cell •" may ac6vate later upon some smulus •" herpes viruses produce latent infec6ons •" examples? –" See table 13.5 G. Detec6on of viruses •" more involved and 6me-consuming than for bacteria.... why? •" can inoculate viruses into fer6lized eggs and looK for characteris6c changes due to viral replicaons •" or inoculate suspensions of material to cell cultures (6ssue cultures) and looK for cytopathic effects (fig. 13.9) •" search for viral an6bodies in the paents’ serum (serological tests) –"Next slide….