DOCSLIB.ORG

Exploring Membrane-Containing Bacteriophages JYVÄSKYLÄ STUDIES in BIOLOGICAL and ENVIRONMENTAL SCIENCE 312

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Exploring Membrane-Containing Bacteriophages JYVÄSKYLÄ STUDIES in BIOLOGICAL and ENVIRONMENTAL SCIENCE 312 JYVÄSKYLÄ STUDIES IN BIOLOGICAL AND ENVIRONMENTAL SCIENCE 312 Sari Mäntynen Something Old, Something New Exploring Membrane-Containing Bacteriophages JYVÄSKYLÄ STUDIES IN BIOLOGICAL AND ENVIRONMENTAL SCIENCE 312 Sari Mäntynen Something Old, Something New Exploring Membrane-Containing Bacteriophages Esitetään Jyväskylän yliopiston matemaattis-luonnontieteellisen tiedekunnan suostumuksella julkisesti tarkastettavaksi yliopiston Ambiotica-rakennuksen salissa YAA303, tammikuun 15. päivänä 2016 kello 12. Academic dissertation to be publicly discussed, by permission of the Faculty of Mathematics and Science of the University of Jyväskylä, in building Ambiotica, hall YAA303, on January 15, 2016 at 12 o’clock noon. UNIVERSITY OF JYVÄSKYLÄ JYVÄSKYLÄ 2016 Something Old, Something New Exploring Membrane-Containing Bacteriophages JYVÄSKYLÄ STUDIES IN BIOLOGICAL AND ENVIRONMENTAL SCIENCE 312 Sari Mäntynen Something Old, Something New Exploring Membrane-Containing Bacteriophages UNIVERSITY OF JYVÄSKYLÄ JYVÄSKYLÄ 2016 Editors Varpu Marjomäki Department of Biological and Environmental Science, University of Jyväskylä Pekka Olsbo, Ville Korkiakangas Publishing Unit, University Library of Jyväskylä Jyväskylä Studies in Biological and Environmental Science Editorial Board Jari Haimi, Anssi Lensu, Timo Marjomäki, Varpu Marjomäki Department of Biological and Environmental Science, University of Jyväskylä Cover picture: Photo of Ylistönrinne campus by Juho Niva; models of φNN P1 core structure and φNN P1 protein by Janne Ravantti. URN:ISBN:978-951-39-6461-0 ISBN 978-951-39-6461-0 (PDF) ISBN 978-951-39-6460-3 (nid.) ISSN 1456-9701 Copyright © 2016, by University of Jyväskylä Jyväskylä University Printing House, Jyväskylä 2016 ABSTRACT Mäntynen, Sari Something old, something new - Exploring membrane-containing bacteriophages Jyväskylä: University of Jyväskylä, 2016, 111 p. (Jyväskylä Studies in Biological and Environmental Science ISSN 1456-9701; 312) ISBN 978-951-39-6460-3 (nid.) ISBN 978-951-39-6461-0 (PDF) Yhteenveto: Jotain uutta, jotain vanhaa – kalvorakenteen sisältävät bakteriofagit Diss. Bacterial viruses, also called bacteriophages or phages, form a remarkably large and diverse group of biological entities. Bacteriophage studies have traditionally centered on tailed non-enveloped species, but a multitude of other virus types is gradually coming to light. Phages with a structural membrane component vary in terms of morphologies, genome types and replication mechanisms. Possibly the most comprehensively characterized membrane- containing phage is enterobacteria infecting PRD1 of the Tectiviridae family. However, there are still unanswered questions concerning its assembly process. This thesis adds new pieces to the puzzle by demonstrating that the subcellular distribution of fluorescently labelled PRD1 proteins is asymmetric, possibly resulting from spatially organized phage protein oligomerization and/or virion assembly. Moreover, PRD1 non-structural proteins P17 and P33 are shown to complement a defect in host co-chaperonin, suggesting a degree of functional redundancy between the viral and bacterial proteins. This thesis also introduces two novel bacteriophages: ǗNN and FLiP infecting Pseudomonas and Flavobacterium species, respectively. The virion of ǗNN contains a tri-segmented dsRNA genome enclosed by an icosahedral protein core and an outermost membrane envelope. Structural and genetic similarities strongly suggest that ǗNN is a new member of the Cystoviridae family. Genetic and structural comparisons among the putative cystoviruses display the conservation of viral “self” components, essential for the formation of functional virions, while the sequences required for host recognition differ. On the other hand, the virion of FLiP consists of an icosahedral protein shell with an internal membrane, uniquely combined with a circular ssDNA genome. FLiP lacks any significant sequence homology to known bacteriophages, but its overall virion structure resembles strikingly that of dsDNA phage PM2, tentatively suggesting a relationship between these phages with different genome types. Keywords: Bacteriophage PRD1; chaperonin complex; Cystoviridae; fluorescent fusion protein; membrane-containing virus; ssDNA phage; virus assembly. Sari Mäntynen, University of Jyväskylä, Department of Biological and Environmental Science, P.O. Box 35, FI-40014 University of Jyväskylä, Finland Author’s address Sari Mäntynen Department of Biological and Environmental Science and Nanoscience Center P.O. Box 35 FI-40014 University of Jyväskylä Finland [email protected] Supervisors Professor Jaana Bamford Department of Biological and Environmental Science and Nanoscience Center P.O. Box 35 FI-40014 University of Jyväskylä Finland Docent Hanna Oksanen Institute of Biotechnology and Department of Biosciences P.O. Box 56 FI-00014 University of Helsinki Finland Reviewers Docent Katri Eskelin Department of Biosciences P.O. Box 56 FI-00014 University of Helsinki Finland Reader Eugene Makeyev MRC Centre for Developmental Neurobiology King´s College London London SE1 1UL United Kingdom Opponent Professor Harri Savilahti Division of Genetics and Physiology Department of Biology FI-20014 University of Turku Finland CONTENTS LIST OF ORIGINAL PUBLICATIONS ABBREVIATIONS 1 INTRODUCTION ................................................................................................... 11 2 REVIEW OF THE LITERATURE .......................................................................... 13 2.1 The good, the bad, and the virus .................................................................. 13 2.2 Virion structure ............................................................................................... 15 2.2.1 Virion morphologies ........................................................................... 16 2.3 Virus assembly ................................................................................................ 17 2.4 Virus classification .......................................................................................... 21 2.5 Viral phylogenetic relationships................................................................... 23 2.5.1 Comparative genomics ....................................................................... 23 2.5.2 Structure-based viral lineages ............................................................ 24 2.6 Bacteriophages ................................................................................................ 28 2.6.1 Membrane-containing bacteriophages ............................................. 29 2.7 Bacteriophage PRD1 – a member of the Tectiviridae family ..................... 31 2.7.1 Tectiviridae – a family of icosahedral, internal membrane– containing dsDNA phages ................................................................. 31 2.7.2 Structure of Enterobacteria phage PRD1 ......................................... 32 2.7.3 Life cycle of PRD1 ................................................................................ 33 2.8 PM2 – the sole member of Corticoviridae ..................................................... 37 2.9 Bacteriophage Ǘ6 and other Cystoviridae family members ....................... 38 2.9.1 Cystoviridae – a family of enveloped phages with segmented dsRNA genome and icosahedral core .............................................. 38 2.9.2 Structure of Pseudomonas phage Ǘ6 ................................................... 40 2.9.3 Life cycle of Ǘ6 ..................................................................................... 42 2.10 Icosahedral bacteriophages with ssDNA genome ..................................... 43 2.11 Subcellular localization of bacterial proteins .............................................. 44 2.11.1 Proteins at the poles of bacterial cells ............................................... 46 2.11.2 Bacteriophages utilize the subcellular architecture ........................ 47 2.12 Use of fluorescent protein fusions in bacterial protein localization studies............................................................................................................... 49 2.13 Chaperones ...................................................................................................... 51 2.13.1 GroEL .................................................................................................... 52 3 AIMS OF THE STUDY ........................................................................................... 55 4 OVERVIEW OF THE METHODS ......................................................................... 56 5 RESULTS AND DISCUSSION .............................................................................. 57 5.1 Fluorescent protein fusions reveal the subcellular localization of bacteriophage PRD1 proteins ....................................................................... 57 5.1.1 Construction of a library of fluorescent protein expression vectors ................................................................................................... 57 5.1.2 Production of fluorescent fusions of PRD1 proteins ...................... 58 5.1.3 Fluorescently labelled PRD1 proteins localize in different parts of the bacterial cell ..................................................................... 60 5.1.4 FRET analysis of interaction between PRD1 vertex proteins P5 and P31 ............................................................................................. 62 5.2 Non-structural proteins P17 and P33 participate in the assembly of bacteriophage
Load more

Recommended publications
  • Changes to Virus Taxonomy 2004
    Arch Virol (2005) 150: 189–198 DOI 10.1007/s00705-004-0429-1 Changes to virus taxonomy 2004 M. A. Mayo (ICTV Secretary) Scottish Crop Research Institute, Invergowrie, Dundee, U.K. Received July 30, 2004; accepted September 25, 2004 Published online November 10, 2004 c Springer-Verlag 2004 This note presents a compilation of recent changes to virus taxonomy decided by voting by the ICTV membership following recommendations from the ICTV Executive Committee. The changes are presented in the Table as decisions promoted by the Subcommittees of the EC and are grouped according to the major hosts of the viruses involved. These new taxa will be presented in more detail in the 8th ICTV Report scheduled to be published near the end of 2004 (Fauquet et al., 2004). Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., and Ball, L.A. (eds) (2004). Virus Taxonomy, VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp. 1258. Recent changes to virus taxonomy Viruses of vertebrates Family Arenaviridae • Designate Cupixi virus as a species in the genus Arenavirus • Designate Bear Canyon virus as a species in the genus Arenavirus • Designate Allpahuayo virus as a species in the genus Arenavirus Family Birnaviridae • Assign Blotched snakehead virus as an unassigned species in family Birnaviridae Family Circoviridae • Create a new genus (Anellovirus) with Torque teno virus as type species Family Coronaviridae • Recognize a new species Severe acute respiratory syndrome coronavirus in the genus Coro- navirus, family Coronaviridae, order Nidovirales
    [Show full text]
  • The Isolation of Viruses Infecting Archaea
    Portland State University PDXScholar Biology Faculty Publications and Presentations Biology 2010 The Isolation of Viruses Infecting Archaea Kenneth M. Stedman Portland State University Kate Porter Mike L. Dyall-Smith Follow this and additional works at: https://pdxscholar.library.pdx.edu/bio_fac Part of the Bacteria Commons, Biology Commons, and the Viruses Commons Let us know how access to this document benefits ou.y Citation Details Stedman, Kenneth M., Kate Porter, and Mike L. Dyall-Smith. "The isolation of viruses infecting Archaea." Manual of aquatic viral ecology. American Society for Limnology and Oceanography (ASLO) (2010): 57-64. This Article is brought to you for free and open access. It has been accepted for inclusion in Biology Faculty Publications and Presentations by an authorized administrator of PDXScholar. Please contact us if we can make this document more accessible: [email protected]. MANUAL of MAVE Chapter 6, 2010, 57–64 AQUATIC VIRAL ECOLOGY © 2010, by the American Society of Limnology and Oceanography, Inc. The isolation of viruses infecting Archaea Kenneth M. Stedman1, Kate Porter2, and Mike L. Dyall-Smith3 1Department of Biology, Center for Life in Extreme Environments, Portland State University, P.O. Box 751, Portland, OR 97207, USA 2Biota Holdings Limited, 10/585 Blackburn Road, Notting Hill Victoria 3168, Australia 3Max Planck Institute of Biochemistry, Department of Membrane Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany Abstract A mere 50 viruses of Archaea have been reported to date; these have been investigated mostly by adapting methods used to isolate bacteriophages to the unique growth conditions of their archaeal hosts. The most numer- ous are viruses of thermophilic Archaea.
    [Show full text]
  • On the Biological Success of Viruses
    MI67CH25-Turner ARI 19 June 2013 8:14 V I E E W R S Review in Advance first posted online on June 28, 2013. (Changes may still occur before final publication E online and in print.) I N C N A D V A On the Biological Success of Viruses Brian R. Wasik and Paul E. Turner Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06520-8106; email: [email protected], [email protected] Annu. Rev. Microbiol. 2013. 67:519–41 Keywords The Annual Review of Microbiology is online at adaptation, biodiversity, environmental change, evolvability, extinction, micro.annualreviews.org robustness This article’s doi: 10.1146/annurev-micro-090110-102833 Abstract Copyright c 2013 by Annual Reviews. Are viruses more biologically successful than cellular life? Here we exam- All rights reserved ine many ways of gauging biological success, including numerical abun- dance, environmental tolerance, type biodiversity, reproductive potential, and widespread impact on other organisms. We especially focus on suc- cessful ability to evolutionarily adapt in the face of environmental change. Viruses are often challenged by dynamic environments, such as host immune function and evolved resistance as well as abiotic fluctuations in temperature, moisture, and other stressors that reduce virion stability. Despite these chal- lenges, our experimental evolution studies show that viruses can often readily adapt, and novel virus emergence in humans and other hosts is increasingly problematic. We additionally consider whether viruses are advantaged in evolvability—the capacity to evolve—and in avoidance of extinction. On the basis of these different ways of gauging biological success, we conclude that viruses are the most successful inhabitants of the biosphere.
    [Show full text]
  • Viruses of Hyperthermophilic Archaea: Entry and Egress from the Host Cell
    Viruses of hyperthermophilic archaea : entry and egress from the host cell Emmanuelle Quemin To cite this version: Emmanuelle Quemin. Viruses of hyperthermophilic archaea : entry and egress from the host cell. Microbiology and Parasitology. Université Pierre et Marie Curie - Paris VI, 2015. English. NNT : 2015PA066329. tel-01374196 HAL Id: tel-01374196 https://tel.archives-ouvertes.fr/tel-01374196 Submitted on 30 Sep 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Université Pierre et Marie Curie – Paris VI Unité de Biologie Moléculaire du Gène chez les Extrêmophiles Ecole doctorale Complexité du Vivant ED515 Département de Microbiologie - Institut Pasteur 7, quai Saint-Bernard, case 32 25, rue du Dr. Roux 75252 Paris Cedex 05 75015 Paris THESE DE DOCTORAT DE L’UNIVERSITE PIERRE ET MARIE CURIE Spécialité : Microbiologie Pour obtenir le grade de DOCTEUR DE L’UNIVERSITE PIERRE ET MARIE CURIE VIRUSES OF HYPERTHERMOPHILIC ARCHAEA: ENTRY INTO AND EGRESS FROM THE HOST CELL Présentée par M. Emmanuelle Quemin Soutenue le 28 Septembre 2015 devant le jury composé de : Prof. Guennadi Sezonov Président du jury Prof. Christa Schleper Rapporteur de thèse Dr. Paulo Tavares Rapporteur de thèse Dr.
    [Show full text]
  • His1 and His2 Are Distantly Related, Spindle-Shaped
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Virology 350 (2006) 228–239 www.elsevier.com/locate/yviro His1 and His2 are distantly related, spindle-shaped haloviruses belonging to the novel virus group, Salterprovirus ⁎ Carolyn Bath 1, Tania Cukalac, Kate Porter, Michael L. Dyall-Smith Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3010, Australia Received 7 December 2005; returned to author for revision 31 January 2006; accepted 2 February 2006 Available online 10 March 2006 Abstract Spindle-shaped viruses are a dominant morphotype in hypersaline waters but their molecular characteristics and their relationship to other archaeal viruses have not been determined. Here, we describe the isolation, characteristics and genome sequence of His2, a spindle-shaped halovirus, and compare it to the previously reported halovirus His1. Their particle dimensions, host-ranges and buoyant densities were found to be similar but they differed in their stabilities to raised temperature, low salinity and chloroform. The genomes of both viruses were linear dsDNA, of similar size (His1, 14,464 bp; His2, 16,067 bp) and mol% G + C (∼40%), with long, inverted terminal repeat sequences. The genomic termini of both viruses are likely to possess bound proteins. They shared little nucleotide similarity and, except for their putative DNA polymerase ORFs, no significant similarity at the predicted protein level. A few of the 35 predicted ORFs of both viruses showed significant matches to sequences in GenBank, and these were always to proteins of haloarchaea. Their DNA polymerases showed 42% aa identity, and belonged to the type B group of replicases that use protein-priming.
    [Show full text]
  • New Tools for Viral Metagenome Comparison and Assembled Virome Analysis Simon Roux1,2, Jeremy Tournayre1,2, Antoine Mahul3, Didier Debroas1,2 and François Enault1,2*
    Roux et al. BMC Bioinformatics 2014, 15:76 http://www.biomedcentral.com/1471-2105/15/76 SOFTWARE Open Access Metavir 2: new tools for viral metagenome comparison and assembled virome analysis Simon Roux1,2, Jeremy Tournayre1,2, Antoine Mahul3, Didier Debroas1,2 and François Enault1,2* Abstract Background: Metagenomics, based on culture-independent sequencing, is a well-fitted approach to provide insights into the composition, structure and dynamics of environmental viral communities. Following recent advances in sequencing technologies, new challenges arise for existing bioinformatic tools dedicated to viral metagenome (i.e. virome) analysis as (i) the number of viromes is rapidly growing and (ii) large genomic fragments can now be obtained by assembling the huge amount of sequence data generated for each metagenome. Results: To face these challenges, a new version of Metavir was developed. First, all Metavir tools have been adapted to support comparative analysis of viromes in order to improve the analysis of multiple datasets. In addition to the sequence comparison previously provided, viromes can now be compared through their k-mer frequencies, their taxonomic compositions, recruitment plots and phylogenetic trees containing sequences from different datasets. Second, a new section has been specifically designed to handle assembled viromes made of thousands of large genomic fragments (i.e. contigs). This section includes an annotation pipeline for uploaded viral contigs (gene prediction, similarity search against reference viral genomes and protein domains) and an extensive comparison between contigs and reference genomes. Contigs and their annotations can be explored on the website through specifically developed dynamic genomic maps and interactive networks. Conclusions: The new features of Metavir 2 allow users to explore and analyze viromes composed of raw reads or assembled fragments through a set of adapted tools and a user-friendly interface.
    [Show full text]
  • Phd Thesis Hyperthermophilic Archaeal Viruses As Novel
    UNIVERSITY OF COPENHAGEN FACULTY OF SCIENCE DANISH ARCHAEA CENTRE PhD thesis Kristine Buch Uldahl Hyperthermophilic archaeal viruses as novel nanoplatforms A cademic supervisor: Xu Peng November 2015 UNIVERSITY OF COPENHAGEN FACULTY OF SCIENCE DANISH ARCHAEA CENTRE PhD thesis Kristine Buch Uldahl Hyperthermophilic archaeal viruses as novel nanoplatforms Academic supervisor: Xu Peng November 2015 Institutnavn: Biologisk Institut Name of department: Department of Biology Section: Functional Genomics Author: Kristine Buch Uldahl Titel: Hypertermofile arkæavirus som nye nanoplatforme Title / Subtitle: Hyperthermophilic archaeal viruses as novel nanoplatforms Subject description: This thesis aims at evaluating archaeal viruses as novel nanoplatforms. The focus will be on investigating the hyperthermophilic archaeal virus, SMV1, to gain insights into the viral life-cycle and to provide a strong knowledge base for developing SMV1 into a nanovector platform. Main supervisor: Associate Professor Xu Peng Co-supervisor: Professor Moein Moghimi Submitted: November 2015 Type: PhD thesis Cover: Top: Kristine Uldahl, sampling trip Yellowstone National Park, Left: TEM image of Sulfolobus monocaudavirus 1, Bottom: Morning Glory Hot spring, Yellowstone National Park Preface This thesis is the product of a three-year PhD project at the Faculty of Science, University of Copenhagen, based at the Danish Archaea Centre, Department of Biology. The thesis has been supervised by associate Professor Xu Peng with heavy involvement from co-supervisor Professor Moein Moghimi (Centre for Pharmaceutical Nanotechnology and Nanotoxiocology (CPNN), University of Copenhagen). Further guidance, collaboration, and advice were received in relation to specific chapters from Mark J. Young and Seth T. Walk. The thesis consists of two parts. The first part is a synopsis which gives an overview of the background and objectives of the thesis, summarizes and discusses the main findings, and outlines some perspectives for future research.
    [Show full text]
  • Chapter 20974
    Genome Replication of Bacterial and Archaeal Viruses Česlovas Venclovas, Vilnius University, Vilnius, Lithuania r 2019 Elsevier Inc. All rights reserved. Glossary RNA-primed DNA replication Conventional DNA Negative sense ( À ) strand A negative-sense DNA or RNA replication used by all cellular organisms whereby a strand has a nucleotide sequence complementary to the primase synthesizes a short RNA primer with a free 3′-OH messenger RNA and cannot be directly translated into protein. group which is subsequently elongated by a DNA Positive sense (+) strand A positive sense DNA or RNA polymerase. strand has a nucleotide sequence, which is the same as that Rolling-circle DNA replication DNA replication whereby of the messenger RNA, and the RNA version of this sequence the replication initiation protein creates a nick in the circular is directly translatable into protein. double-stranded DNA and becomes covalently attached to Protein-primed DNA replication DNA replication whereby the 5′ end of the nicked strand. The free 3′-OH group at the a DNA polymerase uses the 3′-OH group provided by the nick site is then used by the DNA polymerase to synthesize specialized protein as a primer to synthesize a new DNA strand. the new strand. Genomes of Prokaryotic Viruses At present, all identified archaeal viruses have either double-stranded (ds) or single-stranded (ss) DNA genomes. Although metagenomic analyzes suggested the existence of archaeal viruses with RNA genomes, this finding remains to be substantiated. Bacterial viruses, also refered to as bacteriophages or phages for short, have either DNA or RNA genomes, including circular ssDNA, circular or linear dsDNA, linear positive-sense (+)ssRNA or segmented dsRNA (Table 1).
    [Show full text]
  • Evidence to Support Safe Return to Clinical Practice by Oral Health Professionals in Canada During the COVID-19 Pandemic: a Repo
    Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID-19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. November 2020 update This evidence synthesis was prepared for the Office of the Chief Dental Officer, based on a comprehensive review under contract by the following: Paul Allison, Faculty of Dentistry, McGill University Raphael Freitas de Souza, Faculty of Dentistry, McGill University Lilian Aboud, Faculty of Dentistry, McGill University Martin Morris, Library, McGill University November 30th, 2020 1 Contents Page Introduction 3 Project goal and specific objectives 3 Methods used to identify and include relevant literature 4 Report structure 5 Summary of update report 5 Report results a) Which patients are at greater risk of the consequences of COVID-19 and so 7 consideration should be given to delaying elective in-person oral health care? b) What are the signs and symptoms of COVID-19 that oral health professionals 9 should screen for prior to providing in-person health care? c) What evidence exists to support patient scheduling, waiting and other non- treatment management measures for in-person oral health care? 10 d) What evidence exists to support the use of various forms of personal protective equipment (PPE) while providing in-person oral health care? 13 e) What evidence exists to support the decontamination and re-use of PPE? 15 f) What evidence exists concerning the provision of aerosol-generating 16 procedures (AGP) as part of in-person
    [Show full text]
  • 2019.059B.A.V1.Halspiviridae 1Fam
    April 2019 This Word module should be used for all taxonomic proposals. Please complete Part 1 and: either Part 3 for proposals to create new taxa or change existing taxa or Part 2 for proposals of a general nature. Submit the completed Word module, together with the accompanying Excel module named in Part 3, to the appropriate ICTV Subcommittee Chair. The Word module explains and justifies your proposal. The Excel module is a critical document that will be used to implement the proposed taxonomic changes once they are approved and ratified. If proposals presented in the Word module are not presented accurately in the Excel module, the taxonomic changes cannot proceed. For guidance, see the notes written in blue, below, and the Help Notes in file Taxonomic_Proposals_Help_2019. Part 1: TITLE, AUTHORS, etc Code assigned: 2019.059B Short title: Create one new family (Halspiviridae), move one unassigned genus (Salterprovirus) to the family and rename the type species Author(s) and email address(es): List authors in a single line Archives of Virology Provide email address for each author in a single citation format (e.g. Smith AB, Huang C-L, Santos, line separated by semi-colons F) Krupovic M, Oksanen HM, Prangishvili D, Dyall- [email protected]; Smith ML [email protected]; [email protected]; [email protected] Author(s) institutional address(es) (optional): Provide institutional addresses, each on a single line followed by author(s) initials (e.g. University of Woolloomooloo [SAB, HCL]) Institut Pasteur [MK, DP] University of Helsinki [HMO] University of Melbourne [MLD-S] Corresponding author Mart Krupovic ([email protected]) List the ICTV study group(s) that have seen this proposal: A list of study groups and contacts is provided at http://www.ictvonline.org/subcommittees.asp .
    [Show full text]
  • Review Article Lipids of Archaeal Viruses
    Hindawi Publishing Corporation Archaea Volume 2012, Article ID 384919, 8 pages doi:10.1155/2012/384919 Review Article Lipids of Archaeal Viruses Elina Roine and Dennis H. Bamford Department of Biosciences and Institute of Biotechnology, University of Helsinki, P.O. Box 56, Viikinkaari 5, 00014 Helsinki, Finland Correspondence should be addressed to Elina Roine, elina.roine@helsinki.fi Received 9 July 2012; Accepted 13 August 2012 Academic Editor: Angela Corcelli Copyright © 2012 E. Roine and D. H. Bamford. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Archaeal viruses represent one of the least known territory of the viral universe and even less is known about their lipids. Based on the current knowledge, however, it seems that, as in other viruses, archaeal viral lipids are mostly incorporated into membranes that reside either as outer envelopes or membranes inside an icosahedral capsid. Mechanisms for the membrane acquisition seem to be similar to those of viruses infecting other host organisms. There are indications that also some proteins of archaeal viruses are lipid modified. Further studies on the characterization of lipids in archaeal viruses as well as on their role in virion assembly and infectivity require not only highly purified viral material but also, for example, constant evaluation of the adaptability of emerging technologies for their analysis. Biological membranes contain proteins and membranes of archaeal viruses are not an exception. Archaeal viruses as relatively simple systems can be used as excellent tools for studying the lipid protein interactions in archaeal membranes.
    [Show full text]
  • A049p101.Pdf
    Vol. 49: 101–108, 2007 AQUATIC MICROBIAL ECOLOGY Published November 15 doi: 10.3354/ame01141 Aquat Microb Ecol OPENPEN ACCESSCCESS FEATURE ARTICLE Squashed ball-like dsDNA virus infecting a marine fungoid protist Sicyoidochytrium minutum (Thraustochytriaceae, Labyrinthulomycetes) Yoshitake Takao1, Keizo Nagasaki1, Daiske Honda2,* 1National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan 2Department of Biology, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada, Kobe 658-8501, Japan ABSTRACT: Thraustochytrids are cosmopolitan osmo- trophic or heterotrophic microorganisms that play im- portant roles as decomposers, producers of poly- unsaturated fatty acids, and pathogens of mollusks, especially in coastal ecosystems. Sicyoidochytrium min- utum DNA virus (SmDNAV), a novel double-stranded DNA (dsDNA) virus infecting a marine eukaryotic de- composer, S. minutum (formerly Ulkenia minuta), was isolated from the estuary of the Shukugawa River, Hyogo Prefecture, Japan in July 2003, and its basic characteristics were examined. The morphology of the virus particles is ‘squashed ball-like’ (ca. 146 and 112 nm in length and width, respectively) and lacking a tail, distinctive from any other previously known viruses. Virions are formed in the cytoplasm of host TEM of a novel virus (SmDNAV) infecting a heterotrophic cells, frequently accompanied by the disappearance of protist Sicyoidochytrium minutum. (A) Negatively stained the nucleus. The lytic cycle and the burst size were esti- SmDNAV particles (scale bar = 200 nm); (B) thin section of mated at <8 h and 3.6 × 102 to 1.1 × 103 infectious units a SmDNAV-infected cell at 24 h post inoculation (black per host cell, respectively.
    [Show full text]