DOCSLIB.ORG

Abstract PHYSIOLOGICAL STUDIES of ALKALIPHILIC ANAEROBIC

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Abstract PHYSIOLOGICAL STUDIES of ALKALIPHILIC ANAEROBIC Abstract PHYSIOLOGICAL STUDIES OF ALKALIPHILIC ANAEROBIC ORGANOTROPHS IN A SERPENTINIZING SUBSURFACE HABITAT By Crystal R. George October 27, 2014 Director: Dr. Jeffrey McKinnon Department: Biology Microbial habitats in serpentinizing ultramafic rocks represent one of the largest, yet least understood portions of the biosphere, with potentially major consequences for global biogeochemical cycles. Serpentinization is a process where ultramafic rock is uplifted to Earth’s surface through tectonic activity, where it reacts with water to create a highly reducing environment rich in hydrogen, methane, and small abiogenic organic compounds. These compounds may serve as important sources of nutrients and energy to sustain microbial metabolism. While serpentinites can provide the energy to sustain microbial communities, the conditions created by serpentinization can be harsh and challenge the limits of microbial physiology in terms of extreme pH (>11), and availability of terminal electron acceptors. Ongoing culture-independent studies by our research group have identified bacterial taxa related to the order Clostridiales as important components of the highest pH, most reducing subsurface habitats. The goal of this project was to systematically analyze the nutrients necessary to sustain life in a serpentinizing subsurface habitat located on McLaughlin Natural Reserve near Lower Lake, CA. The research consisted of creating microcosm enrichment cultures to evaluate the distribution and environmental controls on Clostridiales species within a subsurface microbial observatory in serpentinizing rocks. Subsequently, the cellular abundance of a novel isolate from these experiments was measured in relation to organic carbon utilization, and utilization of iron and sulfur as terminal electron acceptors. We have also conducted a cursory analysis of annotated biochemical pathways in the genomes of two isolates in relation to respiration and metabolism of carbohydrates. From these experiments we have shown that Clostridiales may play important roles in the biogeochemistry of carbon, iron, and sulfur in serpentinite environments. The research also provides further insight into habitability in these extreme environments and sources of nutrients and energy. Knowledge on the subsurface metabolic capabilities gained through this research has implications for the development of future biotechnologies related to bioremediation and microbial fuel cells. PHYSIOLOGICAL STUDIES OF ALKALIPHILIC ANAEROBIC ORGANOTROPHS IN A SERPENTINIZING SUBSURFACE HABITAT A Thesis Presented To The Faculty of The Department of Biology East Carolina University In Partial Fulfillment of the Requirements for the Degree Master of Science Molecular Biology and Biotechnology By Crystal R George October 27, 2014 © Copyright 2014 Crystal R. George All Rights Reserved PHYSIOLOGICAL STUDIES OF ALKALIPHILIC ANAEROBIC ORGANOTROPHS IN A SERPENTINIZING SUBSURFACE HABITAT by Crystal R. George APPROVED BY: DIRECTOR OF THESIS:___________________________________________ Matthew Schrenk, PhD COMMITTEE MEMBER:__________________________________________________ Anthony Overton, PhD COMMITTEE MEMBER:__________________________________________________ Mary Farwell, PhD COMMITTEE MEMBER:__________________________________________________ Adriana Heimann-Rios, PhD CHAIR OF THE DEPARTMENT OF Biology: ________________________________________________ Jeffrey McKinnon, PhD DEAN OF THE GRADUATE SCHOOL: ________________________________________________ Paul J. Gemperline, PhD ACKNOWLEDGEMENTS First and foremost, I would like to thank Dr. Matthew Schrenk for me giving me the opportunity to conduct research in his lab and meet collaborators through travel to the Coast Range Ophiolite to further conduct hands on research for my project. Allowing me that opportunity gave me a once-in-a-lifetime experience of which I am thankful for. I would also like to thank Dr. Farwell, Dr. Overton and Dr. Heimann-Rios for providing their expertise and guidance with my writing and experimental design. I also extend my gratitude to two of my senior labmates, Melitza Crespo-Medina, and Katrina Twing for providing moral support and guidance over the duration of my research, as well as providing environmental samples and showing me how to collect my own samples during our research trip to CROMO. Secondly, I would like to thank Denise Mayer, Manager of the Genomics Core Facility at East Carolina University for her help with sequencing. In addition, I would like to thank Alyssa Kloysuntia for her help with this project and contributions that she made to our lab. Additionally, I would like to thank Dr. William Brazelton who provided his expertise with several molecular biology methodologies along with structural guidance to several of my experimental designs. Lastly, I would like to thank my family and friends for their encouragement during this program. Funding for this project was provided to Dr. Schrenk from the Division of Research and Graduate Studies at East Carolina University, the NASA Astrobiology Institute through the Carnegie Institution for Science, the Deep Carbon Observatory, and the Alfred P. Sloan Foundation TABLE OF CONTENTS Title Page………………………………………………………………………...... i Copyright…………………………………………………………………………. ii Signature Page……………………………………………………………………. iii Acknowledgements……………………………………………………………..… iv List of Tables............................................................................................................ vii List of Figures.......................................................................................................... viii Chapter 1: Review of Literature………..…..…………………………………….... 1 Biogeochemistry of Serpentinization in Ophiolites…………………...……. 1 Study Site: Coast Range Ophiolite, Lower Lake, California………....…...... 4 Molecular Biology Analysis of Microbial Communities at CROMO…...…. 4 Importance of Dethiobacter alkaliphilus…………....………….…..... 7 Metabolic Capabilities of Alkaliphilic Anaerobes ……………………....…. 8 Iron……………………………………………......…………….……. 8 Sulfur……………………………………………………………..…... 12 Assessment of Metabolic Capabilities at an Isolate Level………………...... 13 Relevance of Experiment…………......………………………………….…. 14 Chapter 2: Research Objectives…………………………………………….....…... 16 Systematically Enrich for Anaerobic Microbial Communities………....…... 1 6 Assess the Diversity of Microcosm Experiments Through TRFLP.....…..…. 17 Partially Characterize a Microorganism from Microcosm Experiment ….... 17 Discover Genes Relevant to Iron, Sulfur and Carbon Metabolic Pathways through Genomic Analyses............................................................................. 17 Chapter 3: Central Hypotheses…………………………………………………...... 18 Chapter 4: Methods……………………………………………………………....... 20 Sample Collection…………………………..………………………………. 20 Culturing and Enrichments………………………………………………….. 22 Cellular Abundance………………………………………………………..... 23 Dilution to Extinction………………………………………………....…….. 24 DNA Extraction……………………………………………......................…. 25 TRFLP Analyses……………………………………............………………. 25 Polymerase Chain Reaction………………………….....…………………… 27 16S rRNA Sequencing…………………………………..............………….. 28 Characterization of an Isolate.......................................................................... 28 Genomics Analyses and Annotation in RAST……………………......…….. 29 Chapter 5: Results………………………………………………………....………. 30 Electron Acceptor Microcosm Treatments…………………………....…….. 30 March Microcosm Experiment………....................................................…… 33 August Microcosm Experiment……….………….................................……. 36 Assessment of Genetic Diversity at CROMO through TRFLP Utilization.... 40 Sugar Utilization by Anaerobic Culture WB…….................…..................... 44 Genomics Comparison of AHT1 and WB and WB1……………………….. 45 Chapter 6: Discussion……………………………………………....……………… 49 References................................................................................................................ 55 APPENDIX A: TRFLP Cluster Dendrograms (Bray-Curtis Similarity)…….. 59 LIST OF TABLES 1. CROMO Wells, Depth, pH and Eh……………………………….………........ 21 2. Restriction Enzymes used in TRFLP…………………………….…………..... 26 3. Beginning and Ending Cellular Abundance – March 2013……………….…... 31 4. Beginning and Ending Cellular Abundance – August 2013…………….…...... 32 5. Normalized (n) values for microcosms March 2013…………………………... 33 6. Statistical correlation between (n) and environmental parameters, March 2013……………………..................................................................................... 35 7. Normalized (n) values for microcosms August 2013…………..……………… 37 8. Statistical correlation between (n) and environmental parameters, August 2013..................................................................................................................... 38 9. Electron Acceptor (n) values for isolate WB....................……………….……... 44 10. Sugar Utilization (n) values for isolate WB…....................…………….………. 45 11. Genes related to respiration in WB, WB1 and AHT1………………..………... 46 12. Genes related to carbohydrate utilization in WB, WB1 and AHT1……..…….. 47 13. Genes related to carbohydrate utilization in WB, WB1 and AHT1..................... 48 LIST OF FIGURES 1. Biogeochemical Processes in a Serpentinite Habitat……………………... 3 2. 16S rRNA sequences mapped to Clostridiales phylogeny……………....... 5 3. Pyrotag sequences from March 2013……………………………………... 6 4. Collection of environmental samples from CROMO…………………….
Load more

Recommended publications
  • Crystalline Iron Oxides Stimulate Methanogenic Benzoate Degradation in Marine Sediment-Derived Enrichment Cultures
    The ISME Journal https://doi.org/10.1038/s41396-020-00824-7 ARTICLE Crystalline iron oxides stimulate methanogenic benzoate degradation in marine sediment-derived enrichment cultures 1,2 1 3 1,2 1 David A. Aromokeye ● Oluwatobi E. Oni ● Jan Tebben ● Xiuran Yin ● Tim Richter-Heitmann ● 2,4 1 5 5 1 2,3 Jenny Wendt ● Rolf Nimzyk ● Sten Littmann ● Daniela Tienken ● Ajinkya C. Kulkarni ● Susann Henkel ● 2,4 2,4 1,3 2,3,4 1,2 Kai-Uwe Hinrichs ● Marcus Elvert ● Tilmann Harder ● Sabine Kasten ● Michael W. Friedrich Received: 19 May 2020 / Revised: 9 October 2020 / Accepted: 22 October 2020 © The Author(s) 2020. This article is published with open access Abstract Elevated dissolved iron concentrations in the methanic zone are typical geochemical signatures of rapidly accumulating marine sediments. These sediments are often characterized by co-burial of iron oxides with recalcitrant aromatic organic matter of terrigenous origin. Thus far, iron oxides are predicted to either impede organic matter degradation, aiding its preservation, or identified to enhance organic carbon oxidation via direct electron transfer. Here, we investigated the effect of various iron oxide phases with differing crystallinity (magnetite, hematite, and lepidocrocite) during microbial 1234567890();,: 1234567890();,: degradation of the aromatic model compound benzoate in methanic sediments. In slurry incubations with magnetite or hematite, concurrent iron reduction, and methanogenesis were stimulated during accelerated benzoate degradation with methanogenesis as the dominant electron sink. In contrast, with lepidocrocite, benzoate degradation, and methanogenesis were inhibited. These observations were reproducible in sediment-free enrichments, even after five successive transfers. Genes involved in the complete degradation of benzoate were identified in multiple metagenome assembled genomes.
    [Show full text]
  • The Keystone Species of Precambrian Deep Bedrock Biosphere the Supplement Related to This Article Is Available Online at Doi:10.5194/Bgd-12-18103-2015-Supplement
    Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Biogeosciences Discuss., 12, 18103–18150, 2015 www.biogeosciences-discuss.net/12/18103/2015/ doi:10.5194/bgd-12-18103-2015 BGD © Author(s) 2015. CC Attribution 3.0 License. 12, 18103–18150, 2015 This discussion paper is/has been under review for the journal Biogeosciences (BG). The keystone species Please refer to the corresponding final paper in BG if available. of Precambrian deep bedrock biosphere The keystone species of Precambrian L. Purkamo et al. deep bedrock biosphere belong to Burkholderiales and Clostridiales Title Page Abstract Introduction L. Purkamo1, M. Bomberg1, R. Kietäväinen2, H. Salavirta1, M. Nyyssönen1, M. Nuppunen-Puputti1, L. Ahonen2, I. Kukkonen2,a, and M. Itävaara1 Conclusions References Tables Figures 1VTT Technical Research Centre of Finland Ltd., Espoo, Finland 2Geological Survey of Finland (GTK), Espoo, Finland anow at: University of Helsinki, Helsinki, Finland J I Received: 13 September 2015 – Accepted: 23 October 2015 – Published: 11 November 2015 J I Correspondence to: L. Purkamo (lotta.purkamo@vtt.fi) Back Close Published by Copernicus Publications on behalf of the European Geosciences Union. Full Screen / Esc Printer-friendly Version Interactive Discussion 18103 Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Abstract BGD The bacterial and archaeal community composition and the possible carbon assimi- lation processes and energy sources of microbial communities in oligotrophic, deep, 12, 18103–18150, 2015 crystalline bedrock fractures is yet to be resolved. In this study, intrinsic microbial com- 5 munities from six fracture zones from 180–2300 m depths in Outokumpu bedrock were The keystone species characterized using high-throughput amplicon sequencing and metagenomic predic- of Precambrian deep tion.
    [Show full text]
  • Draft Genome Sequence of Dethiobacter Alkaliphilus Strain AHT1T, a Gram-Positive Sulfidogenic Polyextremophile Emily Denise Melton1, Dimitry Y
    Melton et al. Standards in Genomic Sciences (2017) 12:57 DOI 10.1186/s40793-017-0268-9 EXTENDED GENOME REPORT Open Access Draft genome sequence of Dethiobacter alkaliphilus strain AHT1T, a gram-positive sulfidogenic polyextremophile Emily Denise Melton1, Dimitry Y. Sorokin2,3, Lex Overmars1, Alla L. Lapidus4, Manoj Pillay6, Natalia Ivanova5, Tijana Glavina del Rio5, Nikos C. Kyrpides5,6,7, Tanja Woyke5 and Gerard Muyzer1* Abstract Dethiobacter alkaliphilus strain AHT1T is an anaerobic, sulfidogenic, moderately salt-tolerant alkaliphilic chemolithotroph isolated from hypersaline soda lake sediments in northeastern Mongolia. It is a Gram-positive bacterium with low GC content, within the phylum Firmicutes. Here we report its draft genome sequence, which consists of 34 contigs with a total sequence length of 3.12 Mbp. D. alkaliphilus strain AHT1T was sequenced by the Joint Genome Institute (JGI) as part of the Community Science Program due to its relevance to bioremediation and biotechnological applications. Keywords: Extreme environment, Soda lake, Sediment, Haloalkaliphilic, Gram-positive, Firmicutes Introduction genome of a haloalkaliphilic Gram-positive sulfur dispro- Soda lakes are formed in environments where high rates portionator within the phylum Firmicutes: Dethiobacter of evaporation lead to the accumulation of soluble carbon- alkaliphilus AHT1T. ate salts due to the lack of dissolved divalent cations. Con- sequently, soda lakes are defined by their high salinity and Organism information stable highly alkaline pH conditions, making them dually Classification and features extreme environments. Soda lakes occur throughout the The haloalkaliphilic anaerobe D. alkaliphilus AHT1T American, European, African, Asian and Australian conti- was isolated from hypersaline soda lake sediments in nents and host a wide variety of Archaea and Bacteria, northeastern Mongolia [10].
    [Show full text]
  • Draft Genome Sequence of Dethiobacter Alkaliphilus Strain AHT1T, a Gram-Positive Sulfidogenic Polyextremophile
    Lawrence Berkeley National Laboratory Recent Work Title Draft genome sequence of Dethiobacter alkaliphilus strain AHT1T, a gram-positive sulfidogenic polyextremophile. Permalink https://escholarship.org/uc/item/7gw7f283 Journal Standards in genomic sciences, 12(1) ISSN 1944-3277 Authors Melton, Emily Denise Sorokin, Dimitry Y Overmars, Lex et al. Publication Date 2017 DOI 10.1186/s40793-017-0268-9 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Melton et al. Standards in Genomic Sciences (2017) 12:57 DOI 10.1186/s40793-017-0268-9 EXTENDED GENOME REPORT Open Access Draft genome sequence of Dethiobacter alkaliphilus strain AHT1T, a gram-positive sulfidogenic polyextremophile Emily Denise Melton1, Dimitry Y. Sorokin2,3, Lex Overmars1, Alla L. Lapidus4, Manoj Pillay6, Natalia Ivanova5, Tijana Glavina del Rio5, Nikos C. Kyrpides5,6,7, Tanja Woyke5 and Gerard Muyzer1* Abstract Dethiobacter alkaliphilus strain AHT1T is an anaerobic, sulfidogenic, moderately salt-tolerant alkaliphilic chemolithotroph isolated from hypersaline soda lake sediments in northeastern Mongolia. It is a Gram-positive bacterium with low GC content, within the phylum Firmicutes. Here we report its draft genome sequence, which consists of 34 contigs with a total sequence length of 3.12 Mbp. D. alkaliphilus strain AHT1T was sequenced by the Joint Genome Institute (JGI) as part of the Community Science Program due to its relevance to bioremediation and biotechnological applications. Keywords: Extreme environment, Soda lake, Sediment, Haloalkaliphilic, Gram-positive, Firmicutes Introduction genome of a haloalkaliphilic Gram-positive sulfur dispro- Soda lakes are formed in environments where high rates portionator within the phylum Firmicutes: Dethiobacter of evaporation lead to the accumulation of soluble carbon- alkaliphilus AHT1T.
    [Show full text]
  • Microbial Diversity in Engineered Haloalkaline Environments Shaped by Shared Geochemical Drivers Observed in Natural Analogues
    Microbial Diversity in Engineered Haloalkaline Environments Shaped by Shared Geochemical Drivers Observed in Natural Analogues Talitha C. Santini,a,b,c Lesley A. Warren,d Kathryn E. Kendrad School of Geography, Planning, and Environmental Management, The University of Queensland, Brisbane, QLD, Australiaa; Centre for Mined Land Rehabilitation, The University of Queensland, Brisbane, QLD, Australiab; School of Earth and Environment, The University of Western Australia, Crawley, WA, Australiac; School of Geography and Earth Sciences, McMaster University, Hamilton, Ontario, Canadad Microbial communities in engineered terrestrial haloalkaline environments have been poorly characterized relative to their nat- ural counterparts and are geologically recent in formation, offering opportunities to explore microbial diversity and assembly in dynamic, geochemically comparable contexts. In this study, the microbial community structure and geochemical characteristics Downloaded from of three geographically dispersed bauxite residue environments along a remediation gradient were assessed and subsequently compared with other engineered and natural haloalkaline systems. In bauxite residues, bacterial communities were similar at the phylum level (dominated by Proteobacteria and Firmicutes) to those found in soda lakes, oil sands tailings, and nuclear wastes; however, they differed at lower taxonomic levels, with only 23% of operational taxonomic units (OTUs) shared with other haloalkaline environments. Although being less diverse than natural analogues,
    [Show full text]
  • Geobacteraceae Are Important Members of Mercury-Methylating Microbial Communities of Sediments Impacted by Waste Water Releases
    The ISME Journal (2018) 12:802–812 https://doi.org/10.1038/s41396-017-0007-7 ARTICLE Geobacteraceae are important members of mercury-methylating microbial communities of sediments impacted by waste water releases 1 2 1 1 1 3 Andrea G. Bravo ● Jakob Zopfi ● Moritz Buck ● Jingying Xu ● Stefan Bertilsson ● Jeffra K. Schaefer ● 4 4,5 John Poté ● Claudia Cosio Received: 19 May 2017 / Revised: 29 September 2017 / Accepted: 18 October 2017 / Published online: 10 January 2018 © The Author(s) 2018. This article is published with open access Abstract Microbial mercury (Hg) methylation in sediments can result in bioaccumulation of the neurotoxin methylmercury (MMHg) in aquatic food webs. Recently, the discovery of the gene hgcA, required for Hg methylation, revealed that the diversity of Hg methylators is much broader than previously thought. However, little is known about the identity of Hg-methylating microbial organisms and the environmental factors controlling their activity and distribution in lakes. Here, we combined high-throughput sequencing of 16S rRNA and hgcA genes with the chemical characterization of sediments impacted by a 1234567890 waste water treatment plant that releases significant amounts of organic matter and iron. Our results highlight that the ferruginous geochemical conditions prevailing at 1–2 cm depth are conducive to MMHg formation and that the Hg- methylating guild is composed of iron and sulfur-transforming bacteria, syntrophs, and methanogens. Deltaproteobacteria, notably Geobacteraceae, dominated the hgcA carrying communities, while sulfate reducers constituted only a minor component, despite being considered the main Hg methylators in many anoxic aquatic environments. Because iron is widely applied in waste water treatment, the importance of Geobacteraceae for Hg methylation and the complexity of Hg- methylating communities reported here are likely to occur worldwide in sediments impacted by waste water treatment plant discharges and in iron-rich sediments in general.
    [Show full text]
  • Carbon and Sulfur Cycling Below the Chemocline in a Meromictic Lake and the Identification of a Novel Taxonomic Lineage in the F
    Carbon and Sulfur Cycling below the Chemocline in a Meromictic Lake and the Identification of a Novel Taxonomic Lineage in the FCB Superphylum, Candidatus Aegiribacteria The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Hamilton, Trinity L., Roderick J. Bovee, Sarah R. Sattin, Wiebke Mohr, William P. Gilhooly, Timothy W. Lyons, Ann Pearson, and Jennifer L. Macalady. 2016. “Carbon and Sulfur Cycling below the Chemocline in a Meromictic Lake and the Identification of a Novel Taxonomic Lineage in the FCB Superphylum, Candidatus Aegiribacteria.” Frontiers in Microbiology 7 (1): 598. doi:10.3389/ fmicb.2016.00598. http://dx.doi.org/10.3389/fmicb.2016.00598. Published Version doi:10.3389/fmicb.2016.00598 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:27320433 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA ORIGINAL RESEARCH published: 27 April 2016 doi: 10.3389/fmicb.2016.00598 Carbon and Sulfur Cycling below the Chemocline in a Meromictic Lake and the Identification of a Novel Taxonomic Lineage in the FCB Superphylum, Candidatus Aegiribacteria Edited by: Trinity L. Hamilton 1*, Roderick J. Bovee 2, Sarah R. Sattin 2, Wiebke Mohr 2 †, Andreas Teske, William P. Gilhooly III 3, Timothy W. Lyons 4, Ann Pearson 2* and Jennifer L. Macalady 5* University of North Carolina at Chapel Hill, USA 1 Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA, 2 Department of Earth and Planetary Sciences, Harvard University, Cambridge, MA, USA, 3 Department of Earth Sciences, Indiana University-Purdue University Reviewed by: Indianapolis, Indianapolis, IN, USA, 4 Department of Earth Sciences, University of California, Riverside, CA, USA, 5 Penn State John R.
    [Show full text]
  • Draft Genome Sequence of Dethiobacter Alkaliphilus Strain AHT1(T), a Gram- Positive Sulfidogenic Polyextremophile
    UvA-DARE (Digital Academic Repository) Draft genome sequence of Dethiobacter alkaliphilus strain AHT1(T), a gram- positive sulfidogenic polyextremophile Melton, E.D.; Sorokin, D.Y.; Overmars, L.; Lapidus, A.L.; Pillay, M.; Ivanova, N.; del Rio, T.G.; Kyrpides, N.C.; Woyke, T.; Muyzer, G. DOI 10.1186/s40793-017-0268-9 Publication date 2017 Document Version Final published version Published in Standards in Genomic Sciences License CC BY Link to publication Citation for published version (APA): Melton, E. D., Sorokin, D. Y., Overmars, L., Lapidus, A. L., Pillay, M., Ivanova, N., del Rio, T. G., Kyrpides, N. C., Woyke, T., & Muyzer, G. (2017). Draft genome sequence of Dethiobacter alkaliphilus strain AHT1(T), a gram-positive sulfidogenic polyextremophile. Standards in Genomic Sciences, 12, [57]. https://doi.org/10.1186/s40793-017-0268-9 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.
    [Show full text]
  • Alkaliphilus Serpentinus Sp. Nov. and Alkaliphilus Pronyensis Sp. Nov
    Alkaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field (New Caledonia) Anne Postec, Marianne Quemeneur, Aurélien Lecoeuvre, Nicolas Chabert, Manon Joseph, Gaël Erauso To cite this version: Anne Postec, Marianne Quemeneur, Aurélien Lecoeuvre, Nicolas Chabert, Manon Joseph, et al.. Al- kaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field (New Caledonia). Sys- tematic and Applied Microbiology, Elsevier, 2021, 44 (2), pp.126175. 10.1016/j.syapm.2020.126175. hal-03107003 HAL Id: hal-03107003 https://hal.archives-ouvertes.fr/hal-03107003 Submitted on 12 Jan 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 1 Alkaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic 2 alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field 3 (New Caledonia). 4 Anne Postec1*, Marianne Quéméneur1, Aurélien Lecoeuvre2, Nicolas Chabert1, Manon 5 Joseph1, Gaël Erauso1 6 1Aix Marseille Univ., Université de Toulon, CNRS, IRD, MIO UM 110, 13288, Marseille, 7 France 8 2 Université de Paris, Institut de Physique du Globe de Paris, CNRS UMR 7154, Paris, France 9 10 *Corresponding author: Dr.
    [Show full text]
  • Microbial Diversity in a Permanently Cold and Alkaline Environment in Greenland
    Microbial diversity in a permanently cold and alkaline environment in Greenland Glaring, Mikkel Andreas; Vester, Jan Kjølhede; Lylloff, Jeanette Eva; Abu Al-Soud, Waleed; Sørensen, Søren Johannes; Stougaard, Peter Published in: PLoS ONE DOI: 10.1371/journal.pone.0124863 Publication date: 2015 Document version Publisher's PDF, also known as Version of record Document license: CC BY Citation for published version (APA): Glaring, M. A., Vester, J. K., Lylloff, J. E., Abu Al-Soud, W., Sørensen, S. J., & Stougaard, P. (2015). Microbial diversity in a permanently cold and alkaline environment in Greenland. PLoS ONE, 10(4), [e0124863]. https://doi.org/10.1371/journal.pone.0124863 Download date: 28. Sep. 2021 RESEARCH ARTICLE Microbial Diversity in a Permanently Cold and Alkaline Environment in Greenland Mikkel A. Glaring1, Jan K. Vester1, Jeanette E. Lylloff1, Waleed Abu Al-Soud2,Søren J. Sørensen2, Peter Stougaard1* 1 Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark, 2 Department of Biology, University of Copenhagen, Copenhagen, Denmark * [email protected] Abstract The submarine ikaite columns located in the Ikka Fjord in Southern Greenland represent a unique, permanently cold (less than 6°C) and alkaline (above pH 10) environment and are home to a microbial community adapted to these extreme conditions. The bacterial and ar- chaeal community inhabiting the ikaite columns and surrounding fjord was characterised by OPEN ACCESS high-throughput pyrosequencing of 16S rRNA genes. Analysis of the ikaite community struc- Citation: Glaring MA, Vester JK, Lylloff JE, Abu ture revealed the presence of a diverse bacterial community, both in the column interior and Al-Soud W, Sørensen SJ, Stougaard P (2015) Microbial Diversity in a Permanently Cold and at the surface, and very few archaea.
    [Show full text]
  • Biotechnological Removal of H2S and Thiols from Sour Gas Streams
    Biotechnological application of microbial sulfidogenesis at haloalkaline conditions João A.B. Sousa Thesis committee Promotor Prof. Dr Alfons J.M. Stams Personal chair at the Laboratory of Microbiology Wageningen University & Research Co-promotors Dr Caroline M. Plugge Associate professor, Laboratory of Microbiology Wageningen University & Research Dr Martijn F.M. Bijmans Manager Europe Wetsus, European Centre of Excellence for Sustainable Water Technology, Leeuwarden Other members Prof. Dr Cees J.N. Buisman, Wageningen University & Research Prof. Dr Piet N.L. Lens, UNESCO-IHE, Delft Prof. Dr Gerard Muyzer, University of Amsterdam Prof. Dr Aharon Oren, The Hebrew University of Jerusalem, Israel This research was conducted under the auspices of the Graduate School for Socio- Economic and Natural Sciences of the Environment (SENSE) Biotechnological application of microbial sulfidogenesis at haloalkaline conditions João A.B. Sousa Thesis submitted in fulfillment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus, Prof. Dr A.P.J. Mol, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Friday 21 April 2017 at 4 p.m. in the Kanselarij, Leeuwarden. João A.B. Sousa Biotechnological application of microbial sulfidogenesis at haloalkaline conditions, 247 pages. PhD thesis, Wageningen University, Wageningen, the Netherlands (2017) With references, with summary in English ISBN 978-94-6343-149-1 DOI 10.18174/410685 Table of contents Chapter
    [Show full text]
  • Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High Ph Katrina I
    University of Rhode Island DigitalCommons@URI Geosciences Faculty Publications Geosciences 2017 Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High pH Katrina I. Twing William J. Brazelton See next page for additional authors Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 License. Follow this and additional works at: https://digitalcommons.uri.edu/geo_facpubs Citation/Publisher Attribution Twing KI, Brazelton WJ, Kubo MDY, Hyer AJ, Cardace D, Hoehler TM, McCollom TM and Schrenk MO (2017) Serpentinization- Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities Adapted to High pH. Front. Microbiol. 8:308. doi: 10.3389/fmicb.2017.00308 Available at: https://doi.org/10.3389/fmicb.2017.00308 This Article is brought to you for free and open access by the Geosciences at DigitalCommons@URI. It has been accepted for inclusion in Geosciences Faculty Publications by an authorized administrator of DigitalCommons@URI. For more information, please contact [email protected]. Authors Katrina I. Twing, William J. Brazelton, Michael D. Y. Kubo, Alex J. Hyer, Dawn Cardace, Tori M. Hoehler, Tom M. McCollom, and Matthew O. Schrenk This article is available at DigitalCommons@URI: https://digitalcommons.uri.edu/geo_facpubs/26 fmicb-08-00308 February 27, 2017 Time: 16:48 # 1 ORIGINAL RESEARCH published: 01 March 2017 doi: 10.3389/fmicb.2017.00308 Serpentinization-Influenced Groundwater Harbors Extremely Low Diversity Microbial Communities
    [Show full text]