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Designed and printed by AIMPRINT 01799 510101 2006 European Fission Yeast Meeting Wellcome Trust Conference Centre, Hinxton, UK 16-18 March 2006 Organizers: Jürg Bähler, Sanger Institute, Hinxton, UK Valerie Wood, Sanger Institute, Hinxton, UK Mitsuhiro Yanagida, Kyoto University, Japan Paul Nurse, Rockefeller University, USA Cover picture: Photo mosaic of double helix made from various S. pombe pictures (J. Bähler) i Sponsors Schedule Overview Main sponsor: Wellcome Trust (http://www.wellcome.ac.uk/) Thu 16th March We thank the following organizations and companies for their help with from 1400 Registration sponsoring this meeting: Cancer Research UK ( h t t p : / / s c i e n c e . c a n c e rre s e a rc h u k . o rg / ) 1500-1630 Reception 1630-1805 Session 1 Biobase ( h t t p : / / w w w. b i o b a s e . d e / ) 1805-2000 Dinner 2000-2130 Session 2 The Genetics Society ( h t t p : / / w w w. g e n e t i c s . o rg . u k / ) Discussion Session: Resources for the The Journal Yeast Fission Yeast ( h t t p : / / w w w 3 . i n t e r s c i e n c e . w i l e y. c o m / Community c g i - b i n / j h o m e / 3 8 9 5 ) Sat 18th March Blackwell Publishing ( h t t p : / / w w w. b l a c k w e l l p u b l i s h i n g . c o m / ) from 0800 Breakfast Singer Instruments Fri 17th March 0930-1100 Session 6 ( h t t p : / / w w w. s i n g e r i n s t . c o . u k / ) 1100-1130 Break Springer from 0730 Breakfast 1130-1300 Session 7 (http://www.springer.com/) 0900-1030 Session 3 1300-1400 Lunch 1030-1100 Break 1100-1230 Session 4 1230-1400 Lunch 1400-1530 Session 5 1530-1600 Break 1600-1900 Poster Session 1900-1930 Pre-dinner drinks 1930 Banquet Dinner from 2130 Entertainment with IMMposters ii iii Programme Thu 16th March, 2000-2130 Fri 17th March, 0900-1030 Fri 17th March, 1100-1230 Session 2: DNA Metabolism II Session 3: Environmental Responses Session 4: Gene Expression Control: Chairs: Tony Carr, Julie Cooper, & Signalling From Chromatin to Proteins Thu 16th March, 1630-1805 Paul Russell Chairs: Paul Young, Olaf Nielsen Chairs: Nic Jones, Karl Ekwall Introduction: Jürg Bähler, Val Wood Anna Hebden (Cooper lab, Søren Kjærrulff Introduction by Nic Jones Session 1: DNA Metabolism I Cancer Research UK) T7 (Nielsen lab, University of (Paterson Institute, UK) Chairs: Tony Carr, Julie Cooper, Surviving DNA damage with dys Copenhagen, Denmark) T10 Paul Russell functional telomeres Cdk-phosphorylation of the HMG- Daniel Lackner domain protein Ste11 controls the (Bähler lab, Zoi Lygerou Edgar Hartsuiker switch from mitosis to meiosis in Sanger Institute, UK) T16 (University of Patras, Greece) T1 (University of Sussex, UK) T8 fission yeast Genome-wide translational control in A stochastic hybrid model for The role of the S. pombe MRN fission yeast DNA replication complex in the removal of covalently Neil Bone linked protein from the DNA (Armstrong lab, Karl Ekwall Chris Norbury University of Sussex, UK) T11 (Karolinska Institute, Sweden) T17 (University of Oxford, UK) T2 Ramsay McFarlane Vtc4p: a regulator of vacuolar size Genome wide roles for histone Involvement of Cid1 poly(A) (University of Wales Bangor, UK) T9 and trafficking in S. pombe modifications and RNAi directed polymerase and the sub-telomeric S. pombe meiotic linear elements: chromatin silencing rqh2+ DNA helicase gene in a the relationship with pre-meiotic Paul Young common pathway following DNA replication and meiotic (Queen’s University, Canada) T12 Anthony Wright inhibition of DNA replication genetic recombination NatB N·-acetylation activity is (Karolinska Institute, Sweden) T18 required for Bsu1p pyridoxine Comparative studies of molecular Benoit Arcangioli transporter function in S. pombe mechanisms that program (Pasteur Institute, France) T3 Discussion Session: transcription The essential role of the homologous Resources for the Fission Yeast Clare Lawrence recombination for replication fork Community (Jones lab, Assen Roguev restart Chairs: Paul Nurse, Mitsuhiro Yanagida Paterson Institute, UK) T13 (Stewart lab, Regulation of S. pombe Atf1 protein The University of Technology Ken’ichi Mizuno Jacky Hayles levels by Sty1-mediated Dresden, Germany) T19 (Carr lab, (Cancer Research UK) phosphorylation and Comparative proteomic analysis of University of Sussex, UK) T4 Status of the S. pombe deletion heterodimerisation with Pcr1 chromatin related complexes in Aberrant chromosome collection S. cerevisiae and S. pombe rearrangement induced by Alison Day replication fork stalling at Mitsuhiro Yanagida (Veal lab, University of José Ayté a palindrome (Kyoto University, Japan) Newcastle, UK) T14 (Universitat Pompeu Fabra, Strain resources in Japan A conserved cysteine of Sty1 has an Spain) T20 Osami Niwa important role in oxidative stress Rem1 expression is regulated at the (Kazusa DNA Research Institute, Val Wood, Jürg Bähler resistance level of transcription and splicing Japan) T5 (Sanger Institute, UK) On the instability of aneuploidy in The future of the S. pombe Janni Petersen Xuefeng Xhu fission yeast GeneDB database (University of Manchester, UK) T15 (Gustafsson lab, Karolinska Institute, Control of cell size at division in Sweden) T21 Paul Russell Paul Nurse response to nutrient availability Genome wide occupancy profile of (The Scripps Research Institute, (Rockefeller University, USA) Mediator and the Srb8-11 module USA) T6 General discussion reveals interactions with Pathways that control the Possible future initiatives? coding regions recruitment of Crb2 to sites of DNA damage iv v Amanda Greenall Iva Tolic-Nørrelykke (Max Planck Inst. Agnes Grallert Hiro Yamano (Whitehall lab, University of of Molecular Cell Biology and (Hagan lab, (Marie Curie Research Newcastle, UK) T22 Genetics, Germany) T28 Paterson Institute, UK) T33 Institute, UK) T37 Hip3 interacts with the HIRA proteins Optical tweezers in fission yeast: Characterization of the fission yeast Mechanisms of the APC/C- Hip1 and Slm9 and is required for mechanism of nuclear and septum CLASP, Peg1 dependent ubiquitylation transcriptional silencing and positioning and proteolysis accurate chromosome segregation Itaru Samejima (Sawin lab, Koji Nagao Fri 17th March, 1600-1900 University of Edinburgh, UK) T34 (Yanagida lab, Fri 17th March, 1400-1530 Identification of an Kyoto University, Japan) T38 Poster Session MTOC-localisation signal domain The domain of securin required for Session 5: Mitotic Spindle in Mto1 stabilization and inhibition of Odd numbers: 1600-1730 separase can have a separase Chairs: Takashi Toda, Iain Hagan Even numbers: 1730-1900 cleavage site by substitutions Sat 18th March, 1130-1300 Anne Kerres Yuko Tonami (Fleig lab, Heinrich-Heine- Session 7: Mitotic and (Murakami lab, Nagoya City Universität, Germany) T23 Sat 18th March, 0930-1100 Meiotic Cell-Division Cycle University, Japan) T39 The conserved kinetochore The mechanism of cell cycle component Spc7 regulates Session 6: Cytoskeleton and Cell Chairs: Shelley Sazer, Chris Norbury progression through meiosis I by microtubule-kinetochore association Morphogenesis the meiosis-specific forkhead and is linked to the Sim4-complex Jérome Wuarin transcription factor Mei4p in Chairs: Jacky Hayles, Ken Sawin (University of Dundee, UK) T35 fission yeast Masamitsu Sato Cell growth and cell cycle: (Toda lab, Cancer Research UK) T24 Introduction by Ken Sawin Cdc2 meets ribosome biogenesis Akira Yamashita Microtubule organisation via (University of Edinburgh, UK) (Yamamoto lab, University of regulation of microtubule-associated Tonje Tvegård Tokyo, Japan) T40 proteins TACC/Alp7 and TOG/Alp14 Stefania Castagnetti (Boye lab, Inst. for Cancer Suppressor mutants of the throughout the cell cycle (Nurse lab, Research, Norway) T36 meiosis I arrest caused by loss of Cancer Research UK) T29 A novel checkpoint reveals a the Mei2p dot Matylda Sczaniecka Generating cell form in fission yeast coupling between general cell (Hardwick lab, growth and the cell cycle Closing remarks University of Edinburgh, UK) T25 Dai Hirata Interactions between the spindle (Hiroshima University, Japan) T30 checkpoint proteins and the Control of growth polarity upon Anaphase Promoting Complex perturbed DNA replication in S. pombe Yolanda Sanchez John Meadows (Universidad de Salamanca, (Millar lab, National Institute for Spain) T31 Medical Research, UK) T26 Rgf1p is a specific Rho1-GEF that Dissociation of mitotic spindle coordinates cell polarization with cell position from the timing of anaphase wall biogenesis in fission yeast onset in fission yeast Anne Paoletti Sylvie Tournier (Curie Intitute, France) T32 (Université Paul Sabatier, Spatial regulation of mid1p cortical France) T27 distribution by pom1p kinase Mechanism controlling perpendicular alignment of the spindle to the axis of cell division in fission yeast vi vii Talk Abstracts We develop here a stochastic hybrid Involvement of Cid1 poly(A) disorder Bloom’s syndrome. The rqh2 model of DNA replication in the cell polymerase and the sub- gene appears to be present in four cycle of the fission yeast. The model + sub-telomeric copies; at least one of A stochastic hybrid model for captures the interplay between discrete telomeric rqh2 DNA helicase these is expressed, following telomere 3 DNA replication. dynamics associated with the firing of gene in a common pathway erosion or replication stress, to origins of replication (pre-replicative, following inhibition of generate an unspliced 7 kb mRNA with K. Koutroumpas1, S. Dimopoulos1, replicating and post-replicative states), a long ORF that includes centromere- 2 1 DNA replication I. Legouras , P. Kouretas , continuous dynamics that arise out of related dh repeat sequences upstream 3 3 C. Heichinger , P. Nurse , the replication process itself 1 from sequences encoding the RecQ 2 1 (continuous progression of the Olivia S Rissland , helicase domain. Transcription of the Z. Lygerou , I. Lygeros Abigail Stevenson1, Li Phing Liew1, replication forks along the genome), 2 3 dh sequences within rqh2 is important and stochastic events due to the Shao-Win Wang , Daniel Lackner , in establishing sub-telomeric 1.
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    Structure and Mechanism of the RNA Polymerase II Transcription Machinery

    Downloaded from genesdev.cshlp.org on October 9, 2021 - Published by Cold Spring Harbor Laboratory Press REVIEW Structure and mechanism of the RNA polymerase II transcription machinery Allison C. Schier and Dylan J. Taatjes Department of Biochemistry, University of Colorado, Boulder, Colorado 80303, USA RNA polymerase II (Pol II) transcribes all protein-coding ingly high resolution, which has rapidly advanced under- genes and many noncoding RNAs in eukaryotic genomes. standing of the molecular basis of Pol II transcription. Although Pol II is a complex, 12-subunit enzyme, it lacks Structural biology continues to transform our under- the ability to initiate transcription and cannot consistent- standing of complex biological processes because it allows ly transcribe through long DNA sequences. To execute visualization of proteins and protein complexes at or near these essential functions, an array of proteins and protein atomic-level resolution. Combined with mutagenesis and complexes interact with Pol II to regulate its activity. In functional assays, structural data can at once establish this review, we detail the structure and mechanism of how enzymes function, justify genetic links to human dis- over a dozen factors that govern Pol II initiation (e.g., ease, and drive drug discovery. In the past few decades, TFIID, TFIIH, and Mediator), pausing, and elongation workhorse techniques such as NMR and X-ray crystallog- (e.g., DSIF, NELF, PAF, and P-TEFb). The structural basis raphy have been complemented by cryoEM, cross-linking for Pol II transcription regulation has advanced rapidly mass spectrometry (CXMS), and other methods. Recent in the past decade, largely due to technological innova- improvements in data collection and imaging technolo- tions in cryoelectron microscopy.