Gyrodactylid Biology, Transmission and Control

Total Page:16

File Type:pdf, Size:1020Kb

Gyrodactylid Biology, Transmission and Control Gyrodactylid Biology, Transmission and Control Bettina Schelkle Organisms and Environment Research Group School of Biological Sciences University of Cardiff A dissertation submitted to the University of Cardiff in accordance with the requirements of the degree of Doctor of Philosophy in the Faculty of Biological Sciences School of Biosciences, Cardiff University September 2012 DECLARATION This work has not been submitted in substance for any other degree or award at this or any other university or place of learning, nor is being submitted concurrently in candidature for any degree or other award. Signed (candidate) Date: 23 February 2013 STATEMENT 1 This thesis is being submitted in partial fulfillment of the requirements for the degree of PhD Signed (candidate) Date: 23 February 2013 STATEMENT 2 This thesis is the result of my own independent work/investigation, except where otherwise stated. Other sources are acknowledged by explicit references. The views expressed are my own. Signed (candidate) Date: 23 February 2013 STATEMENT 3 I hereby give consent for my thesis, if accepted, to be available for photocopying and for inter-library loan, and for the title and summary to be made available to outside organisations. Signed (candidate) Date: 23 February 2013 STATEMENT 4: PREVIOUSLY APPROVED BAR ON ACCESS I hereby give consent for my thesis, if accepted, to be available for photocopying and for inter-library loans after expiry of a bar on access previously approved by the Academic Standards & Quality Committee. Signed (candidate) Date: 23 February 2013 II The contents of Chapters 6-8 and Appendix IV-VII in this thesis are covered by a confidentiality agreement and conform to the ethical policy of WALTHAM Centre for Pet Nutrition. Chapters 2-5 and Appendices I, II and III were independent studies funded by the Biotechnology and Biological Sciences Research Council. All work on vertebrate animals was regulated by Cardiff University ethical policies and UK Home Office Licence procedures. III ABSTRACT Infectious disease in aquaculture is omnipresent, but traditional treatments used against the causative agents often have broad anti-parasitic properties, are not 100 % efficacious, are toxic to humans, fish and the environment, and successful application is challenging. Hence, integrated disease management that combines an understanding of parasite life history and host welfare with treatment is highly recommended. This thesis examined the life history characteristics of a ubiquitous genus of fish ectoparasites, Gyrodactylus species, and tested the efficacy of botanical treatments against gyrodactylids using the guppy (Poecilia reticulata) infected by G. turnbulli or G. bullatarudis as model, with the aim to work towards an integrated management plan for gyrodactylosis in the ornamental fish industry and the hobbyist market. The experiments conducted have shown that (1) gyrodactylids engage in sexual reproduction and on a population level show hybrid fitness and/or inter-strain competition when diverged genotypes are recombined; (2) parasite transmission is mainly driven by direct contact with potential hosts and on dead fish or after detachment gyrodactylids have a window of opportunity to re-attach of over 20 h; (3) extreme habitats provide guppies with a refuge from microbial and gyrodactylid infections in natural populations; (4) anti-parasitic treatment efficacy may be species specific using traditional methods; and (5) salt, garlic, cajuput oil and a combination treatment of the two commercially available treatments Melafix® and Pimafix® are efficacious alternatives for broad anti-parasitic aquarium treatments against gyrodactylids. Due to their efficient reproduction, gyrodactylids are unlikely to be eradicated, as just a single, pregnant worm can trigger a new disease epidemic. However, combining the knowledge on pathogen life history characteristics and host-parasite interactions with effective treatment application will reduce evolutionary pressures on the pathogen and slow its virulence evolution. Overall, this increases the time available for treatment efficacies to be maintained in a manner which corresponds with new drugs being developed as treatment resistance arises. IV ACKNOWLEDGEMENTS Firstly, I would like to thank my supervisor Dr. Jo Cable for her continuous support, encouragement, and extremely valuable advice throughout the whole of my PhD. Thank you also to Dr. Cock van Oosterhout (University of West Anglia) and Prof. David Lloyd (Cardiff University) for discussion and feedback on guppy parasite ecology and statistics and plant extract chemistry, respectively. My PhD was funded through a CASE studentship by the Biotechnology and Biological Sciences Research Council in collaboration with the WALTHAM Centre for Pet nutrition and I would like to thank both organisations for this financial support. I would also like to thank the people at WALTHAM and MARS Fishcare for their help and engagement in Chapters 6-8 of my thesis. Particularly, thank you to my supervisor at WALTHAM, Dr Donna Snellgrove, for ethical advice many times, for arranging visits to MARS Fishcare and WALTHAM, supporting my attendance at the Lindau Nobel Laureate Meeting 2011 and enabling me to carry out GCMS work in the laboratories at WALTHAM. I am also very grateful for the hospitality of Gary Jones and his wife Kate, as well as the Research and Development team at MARS Fishcare in Chalfont (PA, USA): the trip was extremely rewarding both professionally and personally. Thanks also to Lewis Jones and Matt Talbot at WALTHAM for help with the GCMS. Without the organisational help of Ryan Mohammed (University of the West Indies) and his parents’ hospitality research in Trinidad and Tobago would have been impossible. I am also indebted to Andy Shinn and Beppe Paladini (Institute of Aquaculture, Stirling) for an extremely fruitful collaboration when describing Ieredactylus rivulus; thank you for hosting me in your laboratories. Thanks also go to Catrin Williams for her help with Spironucleus vortens, Jo Randall for R advice, Drs Mireille Johnson and Patricia Faria for help with molecular techniques and Dr. Gabrielle Archard for discussions on fish behaviour. Without the practical help of project students, research assistants, IASTE students and our gem of an animal technician much of this work would not exist: Emma Gillingham, Zalina Ali Bashir, Mari Palmer, Amanda Trask, Jo James, Brazilian Jacqui, Sarah Müller and Linda Erlandsson. CRIPES and the Cable Lab; thank you for being such an inspiration and a great group to be part of! On a more personal note, I would like to thank Madeleine, Sigrid, Ashley, Michi, Linda, Catrin, JoJ, Gabrielle, Louis and Layla. Danke Mama, Papa, Stephan, Sarah, für das Vertrauen und den Glauben an mich, selbst wenn ihr nicht immer verstanden habt, was ich mache und warum. Lastly, Richy, thank you so much for being my anchor, believing in me when it was most needed, grounding and supporting me through it all! V CONTENTS DECLARATION........................................................................................................................... II ABSTRACT.................................................................................................................................... IV ACKNOWLEDGEMENTS.......................................................................................................... V CONTENTS.................................................................................................................................... VI LIST OF TABLES......................................................................................................................... XI LIST OF FIGURES....................................................................................................................... XV CHAPTER 1: GENERAL INTRODUCTION............................................................................ 1 1.1 Infectious disease in aquatic environments........................................................................... 1 1.2 Parasite life histories: How do reproduction and transmission contribute to disease persistence and epidemics?................................................................................................... 2 1.3 Infectious disease and its control in food fish and ornamental aquaculture......................... 3 1.4 Integrated infectious disease management and chemical control......................................... 4 1.5 Gyrodactylids........................................................................................................................ 5 1.6 Treatment of gyrodactylid infections in fish......................................................................... 6 1.6.1 Problems with existing treatments.............................................................................. 6 1.6.2 Manual removal of gyrodactylids............................................................................... 9 1.6.3 Summary..................................................................................................................... 10 1.7 The host-parasite system: Gyrodactylus turnbulli and G. bullatarudis infecting common guppies (Poecilia reticulata)................................................................................................. 10 1.8 Thesis aims and hypothesis.................................................................................................. 11 1.9 References............................................................................................................................
Recommended publications
  • Full Text in Pdf Format
    DISEASES OF AQUATIC ORGANISMS Published July 30 Dis Aquat Org Oral pharmacological treatments for parasitic diseases of rainbow trout Oncorhynchus mykiss. 11: Gyrodactylus sp. J. L. Tojo*, M. T. Santamarina Department of Microbiology and Parasitology, Laboratory of Parasitology, Faculty of Pharmacy, Universidad de Santiago de Compostela, E-15706 Santiago de Compostela, Spain ABSTRACT: A total of 24 drugs were evaluated as regards their efficacy for oral treatment of gyro- dactylosis in rainbow trout Oncorhj~nchusmykiss. In preliminary trials, all drugs were supplied to infected fish at 40 g per kg of feed for 10 d. Twenty-two of the drugs tested (aminosidine, amprolium, benznidazole, b~thionol,chloroquine, diethylcarbamazine, flubendazole, levamisole, mebendazole, n~etronidazole,mclosamide, nitroxynil, oxibendazole, parbendazole, piperazine, praziquantel, roni- dazole, secnidazole, tetramisole, thiophanate, toltrazuril and trichlorfon) were ineffective Triclabenda- zole and nitroscanate completely eliminated the infection. Triclabendazole was effective only at the screening dosage (40 g per kg of feed for 10 d), while nitroscanate was effective at dosages as low as 0.6 g per kg of feed for 1 d. KEY WORDS: Gyrodactylosis . Rainbow trout Treatment. Drugs INTRODUCTION to the hooks of the opisthohaptor or to ulceration as a result of feeding by the parasite. The latter is the most The monogenean genus Gyrodactylus is widespread, serious. though some individual species have a restricted distri- Transmission takes place largely as a result of direct bution. Gyrodactyloses affect numerous freshwater contact between live fishes, though other pathways species including salmonids, cyprinids and ornamen- (contact between a live fish and a dead fish, or with tal fishes, as well as marine fishes including gadids, free-living parasites present in the substrate, or with pleuronectids and gobiids.
    [Show full text]
  • A Ovel, Locally Engineered Crude Asphalt Crusher
    Seventh LACCEI Latin American and Caribbean Conference for Engineering and Technology (LACCEI’2009) “Energy and Technology for the Americas: Education, Innovation, Technology and Practice” June 2-5, 2009, San Cristobal. A ovel, Locally Engineered Crude Asphalt Crusher Kenneth Roberts Lake Asphalt of Trinidad and Tobago, [email protected] Kenny Cupid Lake Asphalt of Trinidad and Tobago, [email protected] Kamel Singh The University of Trinidad and Tobago, [email protected] Musti KS Sastry The University of West Indies, Trinidad and Tobago, [email protected] ABSTRACT The ‘Pitch Lake’ located in Trinidad and Tobago, West Indies is perhaps the largest natural and commercial asphalt deposit in the world. The asphalt is mined continuously and processed to produce different commercial products, which are used for different purposes all over the world. The extracted crude asphalt usually will be in large crumbs and difficult to process. This paper presents a safe, low cost, locally engineered crude asphalt crusher to improve the overall processing operations. Keywords: Crude Asphalt, Machine Design, Sustainable Technology, Innovative Solution 1. ITRODUCTIO Trinidad Lake Asphalt is a natural product from the famous Asphalt or “Pitch” Lake located in La Brea, southwest Trinidad Island (southern Carribean), 90 km from the capital Port-of-Spain. This natural resource is actively mined for many years; however the first commercial operations commenced in 1888. It is from this location that refined asphalt, known as Trinidad Lake Asphalt . The lake measures approximately 40 hectares, with an estimated depth up to 76meters at the centre. This wonder holds an estimated ten million tons of pitch; a total of 150 tons of pitch is extracted from the lake per day.
    [Show full text]
  • Chemotherapy of Gastrointestinal Helminths
    Chemotherapy of Gastrointestinal Helminths Contributors J. H. Arundel • J. H. Boersema • C. F. A. Bruyning • J. H. Cross A. Davis • A. De Muynck • P. G. Janssens • W. S. Kammerer IF. Michel • M.H. Mirck • M.D. Rickard F. Rochette M. M. H. Sewell • H. Vanden Bossche Editors H. Vanden Bossche • D.Thienpont • P.G. Janssens UNIVERSITATS- BlfiUOTHElC Springer-Verlag Berlin Heidelberg New York Tokyo Contents CHAPTER 1 Introduction. A. DAVIS A. Pathogenic Mechanisms in Man 1 B. Modes of Transmission 2 C. Clinical Sequelae of Infection 3 D. Epidemiological Considerations 3 E. Chemotherapy 4 F. Conclusion 5 References 5 CHAPTER 2 Epidemiology of Gastrointestinal Helminths in Human Populations C. F. A. BRUYNING A. Introduction 7 B. Epidemiological or "Mathematical" Models and Control 8 C. Nematodes 11 I. Angiostrongylus costaricensis 11 II. Anisakis marina 12 III. Ascaris lumbricoides 14 IV. Capillaria philippinensis 21 V. Enterobius vermicularis 23 VI. Gnathostoma spinigerum 25 VII. Hookworms: Ancylostoma duodenale and Necator americanus . 26 VIII. Oesophagostoma spp 32 IX. Strongyloides stercoralis 33 X. Ternidens deminutus 34 XI. Trichinella spiralis 35 XII. Trichostrongylus spp 38 XIII. Trichuris trichiura 39 D. Trematodes 41 I. Echinostoma spp 41 II. Fasciolopsis buski 42 III. Gastrodiscoides hominis 44 IV. Heterophyes heterophyes 44 V. Metagonimus yokogawai 46 X Contents E. Cestodes 47 I. Diphyllobothrium latum 47 II. Dipylidium caninum 50 III. Hymenolepis diminuta 51 IV. Hymenolepis nana 52 V. Taenia saginata 54 VI. Taenia solium 57 VII. Cysticercosis cellulosae 58 References 60 CHAPTER 3 Epidemiology and Control of Gastrointestinal Helminths in Domestic Animals J. F. MICHEL. With 20 Figures A. Introduction 67 I.
    [Show full text]
  • Paving the Way
    HISTORICAL NOTE Paving the Way On the road of progress, where did the road surfaces were slabs of stone laid upon passing vehicle to the underlying soil with­ asp_halt come from? a strong foundation of rubble or broken out damaging the road surface. Paving ma­ Throughout history, the creation and stone. Other Roman roads used a concrete terial must resist the degradation caused maintenance of roads has been a barome­ made from lime and pozzolana (volcanic by traffic and must also provide a smooth ter for the rise and fall of civilizations. ash). surface for a comfortable ride. Ideally, pav­ While other new science and technology For centuries after the downfall of the Ro­ ing material should resist damage caused developed over the centuries, surprisingly man Empire (about the fifth century A.D.) by changes in temperature, moisture, and little progress was made in the construc­ long-term environmental chemical action. tion of roads or the materials and tech­ In the 1770s two self-taught engineers niques used to surface them. changed roadbuilding methods and mate­ The word "highway" is derived from the rials used throughout Europe-John Roman roads built on a mound of dirt piled The United States and Loudon McAdam and Pierre Tresauguet. up from ditches on either side, raising the Europe have nearly Tresauguet became inspector general of route above the surrounding ground and bridges and roads for France in 1775. He making it a "high way:' The term "road" 5,000,000 total miles of proposed a new theory for a light road sur­ comes from the Anglo-Saxon rad ("to paved roads-most of face, suggesting that the subsoil should ride"), for the path on which travelers support the load of traffic, not the surface rode.
    [Show full text]
  • Reference to Gyrodactylus Salaris (Platyhelminthes, Monogenea)
    DISEASES OF AQUATIC ORGANISMS Published June 18 Dis. aquat. Org. 1 I REVIEW Host specificity and dispersal strategy in gyr odactylid monogeneans, with particular reference to Gyrodactylus salaris (Platyhelminthes, Monogenea) Tor A. Bakkel, Phil. D. Harris2, Peder A. Jansenl, Lars P. Hansen3 'Zoological Museum. University of Oslo. Sars gate 1, N-0562 Oslo 5, Norway 2Department of Biochemistry, 4W.University of Bath, Claverton Down, Bath BA2 7AY, UK 3Norwegian Institute for Nature Research, Tungasletta 2, N-7004 Trondheim. Norway ABSTRACT: Gyrodactylus salaris Malmberg, 1957 is an important pathogen in Norwegian populations of Atlantic salmon Salmo salar. It can infect a wide range of salmonid host species, but on most the infections are probably ultimately lim~tedby a host response. Generally, on Norwegian salmon stocks, infections grow unchecked until the host dies. On a Baltic salmon stock, originally from the Neva River, a host reaction is mounted, limltlng parasite population growth on those fishes initially susceptible. Among rainbow trouts Oncorhynchus mykiss from the sam.e stock and among full sib anadromous arctic char Salvelinus alpjnus, both naturally resistant and susceptible individuals later mounting a host response can be observed. This is in contrast to an anadromous stock of brown trout Salmo trutta where only innately resistant individuals were found. A general feature of salmonid infections is the considerable variation of susceptibility between individual fish of the same stock, which appears genetic in origin. The parasite seems to be generally unable to reproduce on non-salmonids, and on cyprinids, individual behavioural mechanisms of the parasite may prevent infection. Transmission occurs directly through host contact, and by detached gyrodactylids and also from dead fishes.
    [Show full text]
  • The Effects of Inbreeding on Disease Susceptibility: Gyrodactylus Turnbulli Infection of Guppies, Poecilia Reticulata
    Experimental Parasitology 167 (2016) 32e37 Contents lists available at ScienceDirect Experimental Parasitology journal homepage: www.elsevier.com/locate/yexpr Full length article The effects of inbreeding on disease susceptibility: Gyrodactylus turnbulli infection of guppies, Poecilia reticulata * Willow Smallbone a, , Cock van Oosterhout b, Jo Cable a a School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, UK b School of Environmental Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK highlights graphical abstract Inbreeding effects on resistance were tested using the Gyrodactylus-guppy model. Inbred individuals had higher para- site intensity compared to outbred fish. The most inbred individuals were significantly less able to clear the infection. Parasite infections may raise the extinction risk of inbred populations. article info abstract Article history: Inbreeding can threaten population persistence by reducing disease resistance through the accelerated Received 28 October 2015 loss of gene diversity (i.e. heterozygosity). Such inbreeding depression can affect many different fitness- Received in revised form related traits, including survival, reproductive success, and parasite susceptibility. Empirically quanti- 1 April 2016 fying the effects of inbreeding on parasite resistance is therefore important for ex-situ conservation of Accepted 26 April 2016 vertebrates. The present study evaluates the disease susceptibility of individuals bred under three Available online 27 April 2016 different breeding regimes (inbred, crossed with full siblings; control, randomly crossed mating; and fully outbred). Specifically, we examined the relationship between inbreeding coefficient (F-coefficient) Keywords: Gyrodactylidae and susceptibility to Gyrodactylus turnbulli infection in a live bearing vertebrate, the guppy Poecilia fi Ectoparasite reticulata.
    [Show full text]
  • By Philip R. Woodside U.S. Geological Survey Open-File Report 8L This
    UNITED STATES DEPARTMENT OF THE INTERIOR GEOLOGICAL SURVEY THE PETROLEUM GEOLOGY OF TRINIDAD AND TOBAGO By Philip R. Woodside U.S. Geological Survey Open-File Report 8l This report is preliminary and has not been reviewed for conformity with U.S. Geological Survey editorial standards and stratigraphic nomenclature* Any use of trade names is for descriptive purposes only and does not imply endorsment by the USGS. 1981 CONTENTS Page For ewo r d •————————•———-————————————————•————————•—•————•—— Abstract —• Introduction ——————————————————————————————————————————— 1 Structural Geology ————•—-———————•———•—•—————-———•—•——•—— 4 Introduction -——————————————————————————————————————— 4 Structural Areas of Trinidad ——————————————————————————— 5 The Northern Range ——————————•—————————————————————— 5 The Northern (Caroni) Basin —————————————————————————— 6 The Central Range ————————————————————————————————— 6 The Southern Basin (including Naparima Thrust Belt) ———————— 6 Los Bajos fault ———————————————————————————————— 7 The Southern Range ————————————————————————————————— 9 Shale Diapirs ———————————————————————————————————— 10 Stratigraphy ——————————————————————————————————————————— 11 Northern Range and Northern Basin ——————————————————————— 11 Central Range —————————————————————————————————————— 12 Southern Basin and Southern Range —————-————————————————— 14 Suimnary ————————————————————————————————————————————— 18 Oil and Gas Occurrence ———•——————————•——-——————•————-—•—•— 19 Introduction ————•—•————————————————————————-—— 19 Hydrocarbon Considerations
    [Show full text]
  • The Influence of Different Anthelmintics on the Intestinal Epithelial Tissue of Toxocara Canis (Nematoda)
    ISSN 1392-2130. VETERINARIJA IR ZOOTECHNIKA. T. 29 (51). 2005 THE INFLUENCE OF DIFFERENT ANTHELMINTICS ON THE INTESTINAL EPITHELIAL TISSUE OF TOXOCARA CANIS (NEMATODA) Rasa Aukštikalnienė1, Ona Kublickienė1, Antanas Vyšniauskas2 1Vilnius University, M. K. Čiurlionio 21/27, LT-03101, Vilnius, Lithuania tel. +370 52398270 e-mail: [email protected] 2Laboratory for Parasitology, Veterinary Institute of Lithuania Veterinary Academy, Mokslininkų 12, Vilnius, LT–08662, tel. +370 52729727 Summary. Twenty-five puppies naturally infected with Toxocara canis were selected by faecal egg counts for the experiment. Five of them were treated with pyrantel pamoate (14.4 mg/kg BW), five – with albendazole (30 mg/kg BW), five – with levamisole (7.5 mg/kg BW) and five – with nitroskanate (50 mg/kg BW), respectively, and remaining five puppies were served as untreated control. For histological and histochemical investigation all excreted nematodes were collected and the standard technique for investigation of intestinal epithelial tissue was used. The epithelial tissue of T. canis intestine under the action of pyrantel pamoate and nitroscanate changed significantly. The changes were expressed by the appearance of vacuoles in the cytoplasm and by a total disintegration of intestinal epithelial cells. Under the influence of albendazole and levamisole the changes of enterocytes were less significant. The swelling of basal membrane, toddle cytoplasm and blending of fibers in the apical cytoplasm of epithelial cells were registered. The glycogen inclusions and neutral lipids in treated tissue under the action of all used anthelmintics have changed. After treatment with pyrantel pamoate, albendazole and nitroscanate the accumulation of the glycogen deposits in enterocytes lowered gradually and finally dissapeared .
    [Show full text]
  • Microparasite Dispersal in Metapopulations: a Boon Or Bane to the Host Although Connectivity Can Promote Host Species Persistence in a Meta- Population? Proc
    Downloaded from http://rspb.royalsocietypublishing.org/ on September 7, 2018 Microparasite dispersal in rspb.royalsocietypublishing.org metapopulations: a boon or bane to the host population? Christina P. Tadiri1, Marilyn E. Scott2 and Gregor F. Fussmann1 Research 1Department of Biology, and 2Institute of Parasitology, McGill University, Montreal, Quebec, Canada Cite this article: Tadiri CP, Scott ME, CPT, 0000-0002-9305-1960 Fussmann GF. 2018 Microparasite dispersal in metapopulations: a boon or bane to the host Although connectivity can promote host species persistence in a meta- population? Proc. R. Soc. B 285: 20181519. population, dispersal may also enable disease transmission, an effect further complicated by the impact that parasite distribution may have on host–parasite http://dx.doi.org/10.1098/rspb.2018.1519 population dynamics. We investigated the effects of connectivity and initial parasite distribution (clustered or dispersed) on microparasite–host dynamics in experimental metapopulations, using guppies and Gyrodactylus turnbulli. We created metapopulations of guppies divided into four subpopulations Received: 5 July 2018 and introduced either a low level of parasites to all subpopulations (dispersed) Accepted: 3 August 2018 or a high level of parasites to one subpopulation (clustered). Controlled migration among subpopulations occurred every 10 days. In additional trials, we introduced low or high levels of parasites to isolated populations. Parasites persisted longer in metapopulations than in isolated populations. Mortality Subject Category: was lowest in isolated populations with low-level introductions. The interaction of connectivity and initial parasite distribution influenced parasite abundance. Ecology With low-level introductions, connectivity helped the parasite persist longer but had little effect on the hosts.
    [Show full text]
  • Risk Analysis and Potential Implications of Exotic Gyrodactylus
    RISK ANALYSIS AND POTE NTIAL TRANSMISSION AND IMPLICATIONS OF EXOTIC GYRODACTYLUS SPECIES ON CULTURED AND WILD CYPRINIDS IN THE WESTERN CAPE, SOUTH AFRICA By: Monique Rochelle Maseng Submitted in fulfillment of the requirements for the degree of Magister Scientiae Department of Biodiversity and Conservation Biology Faculty of Natural Sciences University of the Western Cape Bellville Supervisors: Dr Kevin Christison (Department of Biodiversity and Conservation, University of the Western Cape) Prof Charles Griffiths (Zoology Department, University of Cape Town) September 2010 Declaration I declare that this is my own work, that Risk analysis and potential implications of exotic Gyrodactylus species on cultured and wild cyprinids in the Western Cape, South Africa has not been submitted for any degree or examination in any other university, and that all the sources I have used or quoted have been indicated and acknowledged by complete references. ………………………………… Monique Rochelle Maseng September 2010 i Keywords Challenge infections Gyrodactylus Gyrodactylus burchelli Host specificity Morphological variation Phenotypic plasticity Pseudobarbus sp. Risk analysis ii Abstract The expansion of the South African aquaculture industry coupled with the lack of effective parasite management strategies may potentially have negative effects on both the freshwater biodiversity and economics of the aquaculture sector. Koi and goldfish are notorious for the propagation of parasites worldwide, some of which have already infected indigenous fish in South Africa. Koi and goldfish have been released into rivers in South Africa since the 1800’s for food and sport fish and have since spread extensively. These fish are present in most of the river systems in South Africa and pose an additional threat the indigenous cyprinids in the Western Cape.
    [Show full text]
  • Sheet1 Page 1 a Abamectin Acetazolamide Sodium Adenosine-5-Monophosphate Aklomide Albendazole Alfaxalone Aloe Vera Alphadolone A
    Sheet1 A Abamectin Acetazolamide sodium Adenosine-5-monophosphate Aklomide Albendazole Alfaxalone Aloe vera Alphadolone Acetate Alpha-galactosidase Altrenogest Amikacin and its salts Aminopentamide Aminopyridine Amitraz Amoxicillin Amphomycin Amphotericin B Ampicillin Amprolium Anethole Apramycin Asiaticoside Atipamezole Avoparcin Azaperone B Bambermycin Bemegride Benazepril Benzathine cloxacillin Benzoyl Peroxide Benzydamine Bephenium Bephenium Hydroxynaphthoate Betamethasone Boldenone undecylenate Boswellin Bromelain Bromhexine 2-Bromo-2-nitropan-1, 3 diol Bunamidine Buquinolate Butamisole Butonate Butorphanol Page 1 Sheet1 C Calcium glucoheptonate (calcium glucoheptogluconate) Calcium levulinate Cambendazole Caprylic/Capric Acid Monoesters Carbadox Carbomycin Carfentanil Carnidazole Carnitine Carprofen Cefadroxil Ceftiofur sodium Centella asiatica Cephaloridine Cephapirin Chlorine dioxide Chlormadinone acetate Chlorophene Chlorothiazide Chlorpromazine HCl Choline Salicylate Chondroitin sulfate Clazuril Clenbuterol Clindamycin Clomipramine Clopidol Cloprostenol Clotrimazole Cloxacillin Colistin sulfate Copper calcium edetate Copper glycinate Coumaphos Cromolyn sodium Crystalline Hydroxycobalamin Cyclizine Cyclosporin A Cyprenorphine HCl Cythioate D Decoquinate Demeclocycline (Demethylchlortetracycline) Page 2 Sheet1 Deslorelin Desoxycorticosterone Pivalate Detomidine Diaveridine Dichlorvos Diclazuril Dicloxacillin Didecyl dimethyl ammonium chloride Diethanolamine Diethylcarbamazine Dihydrochlorothiazide Diidohydroxyquin Dimethylglycine
    [Show full text]
  • Asfalty Naturalne – Charakterystyka I Zastosowanie
    ARCHIWUM INSTYTUTU INŻYNIERII LĄDOWEJ Nr 27 ARCHIVES OF INSTITUTE OF CIVIL ENGINEERING 2018 NATURAL ASPHALTS – PROPERTIES AND USE 1 Marcin BILSKI Poznan University of Technology, Faculty of Civil and Environmental Engineering The article describes and illustrates natural asphalts according to the classification due to the solubility of carbon disulphide. The review of bitumens and the sources of their acquisition aims to systematize knowledge on this subject in terms of practical application. Exemplary applications on an industrial scale, discussed natural asphalts with particular emphasis on their use in road construction have been presented. 1. INTRODUCTION The term “asphalt” (bitumen) is used both in the description of artificial as- phalt obtained in petroleum processing (the so-called petroleum bitumen, refined bitumen), as well as the raw resources in form of natural deposits. Natural as- phalt is composed of organic and mineral substances with water [1]. The organic part is a compound mixture of hydrocarbons, asphaltites and resins, accompa- nied by considerable amounts of sulphur, oxygen, nitrogen, as well as vanadium, nickel and iron in its carbon structure [2]. Following the definition provided in the EN 12597 standard [3], natural asphalt is relatively hard, can be found in natural deposits, and is often mixed with fine or very fine mineral material, which usually appears in solid form in the temperature of 25°C, and as viscous liquid in 175°C. 2. CLASSIFICATION AND USE OF NATURAL ASPHALTS The primary method for classifying natural asphalts is their ability to dis- solve in CS2 (carbon disulphide) [2, 4]. Fig. 1 shows a classification of natural asphalts in relation to their solubility in CS2, and provides examples of deposits or names of minerals they can be found in.
    [Show full text]