SMYD4 Antibody

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Efficient Professional Protein and Antibody Platforms SMYD4 Antibody Basic information: Catalog No.: UPA06235 Source: Rabbit Size: 50ul/100ul Clonality: Polyclonal Concentration: 1mg/ml Isotype: IgG Purification: The antibody was purified by immunogen affinity chromatography. Useful Information: WB: 1:500 - 1:2000 Applications: IHC: 1:50-1:200 IF/IC: 1:50-1:100 Reactivity: Human, Mouse Specificity: Recognizes endogenous levels of SMYD4 protein. Immunogen: Recombinant full length protein of human SMYD4 Description: Rabbit polyclonal antibody to SMYD4 Uniprot: Q8IYR2(Human), Q8BTK5(Mouse) BiowMW: Refer to Figures Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% Buffer: glycerol, and 0.01% sodium azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Western blot analysis of SMYD4 expression in mouse brain (A), mouse ovary (B), rat kidney (C) whole cell lysates. Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Immunohistochemical analysis of SMYD4 staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counter- stained with haematoxylin and mounted with DPX. Immunofluorescent analysis of SMYD4 staining in MCF7 cells. Formalin-fixed cells were permea- bilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] .

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Noelia Díaz Blanco
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