Figure S1. Heat map of GSE79973, comparing between 10 gastric cancer tissues and 10 normal tissues. The GSE79973 included 10 patients. Every sample was from the same patient. These were patient 1 (32C,33N), patient 2 (34C,35N)….patient 9 (48C,49N), patient 10 (50C,51N), respectively. Columns with even numbers are for gastric cancer tissue and those with odd numbers are for normal tissues. C, cancer; N, normal. Figure S2. mRNA expression levels of histone modification‑​ associated in different types of cancer according to the Oncomine database. Upregulation is indicated in red and downregulation in blue. Table SI. Basic characteristics and function of histone modification enzymes and associated genes.

A, HDACs and associated genes

Protein Location Exon mass (kDa) Encoding and biological function

HDAC1 1p35.2‑p35.1 14 55.11 Encodes a component of the HDAC complex. Interacts with retinoblastoma tumor‑suppressor protein and controls cell proliferation and differentiation. Together with metastasis‑associated protein‑2, it deacetylates and modulates its effect on cell growth and apoptosis. HDAC2 6q21 17 55.37 Performs deacetylation of lysine residues at the N‑terminal regions of core histones (H2A, H2B, H3 and H4). Forms transcriptional repressor complexes by associating with , including YY1. Participates in transcriptional regulation, cell cycle progression and developmental events. HDAC3 5q31.3 15 48.81 HDAC with a potential tumor suppressor gene function. Represses transcription when tethered to a promoter through its binding with YY1. Downregulates the function of p53 and modulates cell growth and apoptosis. HDAC4 2q37.3 37 106.39 Class II of the HDAC/AcuC/aphA family. Represses transcription; binds DNA through transcription factors MEF2C and MEF2D. Interacts with RbAp48 and HDAC3. HDAC5 17q21.31 30 122.02 Class II of the HDAC/AcuC/aphA family. Represses transcription. Coimmunoprecipitates only with HDAC3 family member. Interacts with proteins to repress expression of MEF2‑dependent genes. HDAC6 Xp11.23 32 131.46 Class II of the HDAC/AcuC/aphA family. Contains an internal duplication of two catalytic domains, which appear to function independently of each other. Possesses HDAC activity to repress transcription. HDAC7 12q13.11 32 92.38 HDAC promoting repression mediated via the transcriptional corepressor SMRT. HDAC8 Xq13.1 16 41.76 Class I of the HDAC family, catalyzing the deacetylation of lysine residues in the histone N‑terminal tails, repressing transcription in large multiprotein complexes with transcriptional corepressors. HDAC9 7p21.1 38 111.33 HDAC family, repressing MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may have a role in hematopoiesis. HDAC10 22q13.33 20 71.46 HDAC family, deacetylating lysine residues on the N‑terminal part of the core histones. HDAC11 3p25.1 14 39.19 Class IV HDAC, localized to the nucleus and may be involved in regulating the expression of interleukin 10. SIRT1 10q21.3 11 47.06 Class I of the sirtuin family. May function as intracellular regulatory proteins with mono‑ADP‑ribosyltransferase activity. SIRT2 19q13.2 17 39.52 Class I of the sirtuin family. May function as intracellular regulatory proteins with mono‑ADP‑ribosyltransferase activity. SIRT3 11p15.5 9 43.58 Class I of the sirtuin family. May function as intracellular regulatory proteins with mono‑ADP‑ribosyltransferase activity. SIRT4 12q24.23‑q24.31 5 35.19 Class IV of the sirtuin family. May function as intracellular regulatory proteins with mono‑ADP‑ribosyltransferase activity. SIRT5 6p23 15 33.89 Class III of the sirtuin family. May function as intracellular regulatory proteins with mono‑ADP‑ribosyltransferase activity. SIRT6 19p13.3 9 36.44 A member of the sirtuin family of NAD‑dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. Localized to the nucleus and exhibits ADP‑ribosyl transferase and HDAC activities. Participates in DNA repair, maintenance of telomeric chromatin, inflammation, as well as in lipid and glucose metabolism. SIRT7 17q25.3 11 44.9 Class IV of the sirtuin family. May function as intracellular regulatory proteins with mono‑ADP‑ribosyltransferase activity. Table SI. Continued.

B, HATs and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

HAT1 2q31.1 12 49.52 A type B HAT that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, has an important role in replication‑dependent chromatin assembly. Specifically, this HAT may acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. KAT2A 17q21.2 18 93.94 Also known as GCN5. A HAT that functions primarily as a transcriptional activator. It also functions as a repressor of NF‑κB by promoting ubiquitination of the NF‑κB subunit RELA in a HAT‑independent manner. KAT2B 3p24.3 19 93.03 Possesses HAT activity. Associates with p300/CBP by binding to numerous sequence‑specific factors, including c‑jun or the adenoviral oncoprotein E1A, involved in cell growth and/or differentiation. Has a direct role in transcriptional regulation. KAT5 11q13.1 13 53.08 The MYST family of HATs, regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. Participation in DNA repair and apoptosis. Important role in signal transduction. CREBBP 16p13.3 33 265.44 Transcriptional coactivation. Binding to CREB, this HAT acts as a scaffold to stabilize additional protein interactions with the transcription complex. Very high sequence similarity with protein p300 in its bromodomain, cysteine‑histidine‑rich regions, and HAT domain. Mutations in this gene cause RTS. Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. EP300 22q13.2 31 264.25 Encodes the adenovirus E1A‑associated cellular p300 transcriptional co‑activator protein. A HAT regulating transcription via chromatin remodeling, mediating cAMP gene regulation by binding specifically to phosphorylated CREB protein. A co‑activator of hypoxia‑inducible factor 1 alpha, thus stimulating hypoxia‑induced genes such as VEGF. Defects in this gene are a cause of RTS and may also have a role in epithelial cancer. TAF1 Xq13.1 45 20.89 Coordinating initiation of transcription by RNA polymerase II. The basal TFIID, binding to core promoter sequences encompassing the transcription start site and also binding to activators and other transcriptional regulators. Contain two independent protein kinase domains at the N‑ and C‑terminals, but also possess acetyltransferase activity and may act as a ubiquitin‑activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X‑linked, a dystonia‑parkinsonism disorder. ELP3 8p21.1 18 The catalytic subunit of the HAT elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons. NCOA1 2p23.3 24 156.8 A transcriptional coactivator for steroid and nuclear hormone receptors. A member of the p160/steroid coactivator family, which has HAT activity and contains a nuclear localization signal, as well as bHLH and PAS domains. Also binds nuclear receptors directly and stimulates the transcriptional activities in a hormone‑dependent manner. NCOA2 8q13.3 32 159.2 A transcriptional coactivator for nuclear hormone receptors, including steroid, thyroid, retinoid and vitamin D receptors. Also an intermediary factor for the ligand‑dependent activity of these nuclear receptors, which regulate their target genes upon binding of cognate response elements. NCOA3 20q13.12 23 154.81 A coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. A HAT recruiting p300/CBP‑associated factor and CREB binding protein as part of a multisubunit coactivation complex. Table SI. Continued.

B, HATs and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

CLOCK 4q12 28 Participates in the regulation of circadian rhythms. A transcription factor of the bHLH family that possesses DNA‑binding and HAT activity. Forms a heterodimer with ARNTL (BMAL1) that binds E‑box enhancer elements upstream of period circadian regulator 1, 2 and 3 and cryptochrome 1 and 2 genes, and activates transcription of these genes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome.

C, HMT‑encoding and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

SMYD1 2p11.2 10 56.63 ‑ SMYD2 1q32.3 12 49.77 SET domain‑containing proteins, including SMYD2, catalyze lysine methylation. SMYD3 1q44 30 ‑ A HMT that functions in RNA polymerase II complexes by interaction with a specific RNA helicase. SMYD4 17p13.3 14 89.18 ‑ SMYD5 2p13.2 13 34.42 ‑ PRMT1 19q13.33 13 39.61 A member of the PRMT family, which is involved in post‑translational modification of target proteins in numerous biological processes. It is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may have a role in numerous types of cancer. PRMT2 21q22.3 12 49.01 ‑ PRMT3 11p15.1 18 59.91 A member of the PRMT family, which catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. PRMT5 14q11.2 17 72.69 Member of the methyltransferase family, which catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. Participating in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. PRMT6 1p13.3 1 41.94 A member of the PRMT family, which catalyzes the sequential transfer of a methyl group from S‑adenosyl‑L‑methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S‑adenosyl‑L‑homocysteine. PRMT7 16q22.1 22 78.47 A member of the PRMT family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. Participates in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis and regulation of the Wnt signaling pathway. May promote breast cancer cell invasion and metastasis in human patients. PRMT8 12p13.32 12 45.3 Arginine methylation is a widespread post‑translational modification mediated by PRMTs, including PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization and possibly protein translation CARM1 19p13.2 17 65.86 A member of the PRMT family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin‑associated proteins and is involved in regulation of . Table SI. Continued.

C, HMT‑encoding and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

NSD1 5q35.3 31 267.39 Containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals, 4 PHD finger regions, and a proline‑rich region. Enhances transactivation. Acts as a nucleus‑localized, basic transcriptional factor or also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. SETD2 3p21.31 26 231.28 A class of huntingtin‑interacting proteins characterized by WW motifs. A HMT that is specific for lysine‑36 of histone H3, and methylation of this residue is associated with active chromatin. Also contains a novel transcriptional activation domain associated with hyperphosphorylated RNA polymerase II. SETD3 14q32.2 19 67.27 ‑ DOT1L 19p13.3 30 ‑ A HMT that methylates lysine‑79 of histone H3. It has no activity against free core histones, but exhibits significant HMT activity against nucleosomes. EZH1 17q21.2 21 85.33 Component of a noncanonical PRC2 that mediates methylation of histone H3 lys27 and functions in the maintenance of embryonic stem cell pluripotency and plasticity EZH2 7q36.1 25 ‑ A member of the polycomb‑group family, which forms multimeric protein complexes and is involved in maintaining the transcriptional repressive state of genes over successive cell generations. Associates with the embryonic ectoderm development protein, the VAV1 oncoprotein and the X‑linked nuclear protein. Participates in the hematopoietic and central nervous systems. EHMT1 9q34.3 38 86.72 A HMT that methylates the lysine‑9 position of histone H3, which marks the genomic region packaged with these methylated histones for transcriptional repression. May be involved in the silencing of ‑ and ‑responsive genes and may therefore have a role in the G0/G1 cell cycle transition. EHMT2 6p21.33 29 132.4 A methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA . ASH1L 1q22 33 332.26 A member of the trithorax group of transcriptional activators. Contains four AT hooks, a SET domain, a PHD‑finger motif and a bromodomain. It is localized to numerous small speckles in the nucleus and also to cell‑cell tight junctions. SUV39H2 10p13 8 39.95 ‑ SUV39H1 Xp11.23 7 47.91 An evolutionarily conserved protein containing an N‑terminal chromodomain and a C‑terminal SET domain. A HMT that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause instability. MLL 11q23.3 37 432.12 A transcriptional coactivator that has an essential role in regulating gene expression during early development and hematopoiesis. The SET domain is responsible for its histone H3 lysine 4 methyltransferase activity, which mediates chromatin modifications associated with epigenetic transcriptional activation. It is processed by the enzyme Taspase 1 into two fragments, MLL‑C and MLL‑N, which assemble into different multiprotein complexes that regulate the transcription of specific target genes, including numerous HOX genes. MLL2 12q13.12 56 593.54 A HMT that methylates the Lys‑4 position of histone H3. It is also part of a large protein complex called ASCOM, which has been indicated to be a transcriptional regulator of the beta‑globin and genes. Mutations in this gene have been demonstrated to be a cause of Kabuki syndrome. MLL3 7q36.1 65 541.49 A member of the myeloid/lymphoid or MLL family that encodes a nuclear protein with an AT hook DNA‑binding domain, a DHHC‑type , six PHD‑type zinc fingers, a SET domain, a post‑SET domain and a RING‑type zinc finger. Also a member of the ASCOM, which possesses histone methylation activity and is involved in transcriptional coactivation. Table SI. Continued.

C, HMT‑encoding and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

MLL5 7q22.3 33 205.01 A member of the myeloid/lymphoid or MLL family and encodes a protein with an N‑terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. SETD1A 16p11.2 22 186.06 A component of a HMT complex that produces mono‑, di‑ and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains and an RNA recognition motif domain, and is a member of the class V‑like SAM‑binding methyltransferase superfamily. SETD1B 12q24.31 19 212.83 A component of a HMT complex that produces trimethylated histone H3 at Lys4 SETD5 3p25.3 36 99.1 May function as a HMT. Mutations in this gene have been associated with an autosomal‑dominant form of intellectual disability. SETD7 4q31.1 10 40.73 ‑ SETD8 12q24.31 11 39.29 A protein‑lysine N‑methyltransferase that monomethylates Lys‑20 of histone H4 to cause transcriptional repression of certain genes. It is required for cell proliferation and has a role in chromatin condensation. SETMAR 3p26.1 4 39.15 Contains an N‑terminal histone‑lysine N‑methyltransferase domain and a C‑terminal mariner transposase domain. Binds DNA and functions in DNA repair activities. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. SETDB1 1q21.3 22 143.01 A HMT that regulates histone methylation, gene silencing and transcriptional repression. A target for the treatment of Huntington’s disease, given that gene silencing and transcriptional dysfunction likely have a role in the pathogenesis. SETDB2 13q14.2 15 80.68 A member of a family of proteins that contain a methyl‑CpG‑binding domain and a SET domain and functions as a HMT. It is recruited to heterochromatin and has a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. WHSC1L1 8p11.23 25 161.64 Linked to the WHS candidate‑1 gene and encodes a protein with PWWP domains. Methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. WHSC1 4p16.3 29 62.87 Contains four domains present in other developmental proteins: A PWWP domain, an HMG box, a SET domain and a PHD‑type zinc finger. It is expressed ubiquitously in early development. Aberrant expression of this gene is associated with WHS. SUV420H1 11q13.2 14 54.94 Contains a SET domain. SET domains appear to be protein‑protein interaction domains that mediate interactions with a family of proteins that display similarity with dual‑specificity phosphatases. SUV420H2 19q13.42 10 52.12 SUV420H2 and the associated enzyme SUV420H1 function as HMTs that specifically trimethylate nucleosomal histone H4 on lysine 20. PRDM1 6q21 11 ‑ Acts as a repressor of beta‑interferon gene expression. Binds specifically to the PRDI of the beta‑IFN gene promoter. Transcription of this gene increases upon viral induction. PRDM2 1p36.21 17 ‑ A tumor suppressor gene and a member of a nuclear histone/protein methyltransferase superfamily. Binds to Rb protein, estrogen receptor and the TPA‑responsive element of the heme‑oxygenase‑1 gene. Participates in transcriptional regulation, acting as a transcriptional activator of the heme‑oxygenase‑1 gene. A specific effector of estrogen action. PRDM4 12q23.3 12 ‑ A transcription factor of the PR‑domain protein family. Contains a PR domain and multiple zinc finger motifs. Involved in cell differentiation and tumorigenesis. PRDM5 4q27 23 ‑ A transcription factor of the PR‑domain protein family. Contains a PR‑domain and multiple zinc finger motifs. Involved in cell differentiation and tumorigenesis. PRDM7 16q24.3 11 ‑ A member of a family of proteins that may have roles in transcription and other nuclear processes. Contains a KRAB domain ‑A box and a SET domain and may function as a HMT. Table SI. Continued.

C, HMT‑encoding and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

PRDM9 5p14.2 11 ‑ A zinc finger protein with HMT activity that catalyzes histone H3 lysine 4 trimethylation during the meiotic prophase. Contains multiple domains, including a KRAB domain, an SSX repression domain, a PR domain, a PR/SET domain and a tandem array of C2H2 zinc fingers. PRDM10 11q24.3 22 ‑ A transcription factor that contains C2H2‑type zinc fingers. Also contains a positive regulatory domain, which has been identified in several other zinc‑finger transcription factors, including those involved in B‑cell differentiation and tumor suppression. PRDM11 11p11.2 13 ‑ ‑ PRDM12 9q34.12 5 ‑ A transcriptional regulator of sensory neuronal specification that has a critical role in pain perception. Contains an N‑terminal PR domain, a SET domain and three C‑terminal C2H2 zinc finger DNA‑binding domains. Naturally occurring mutations in this gene are associated with congenital insensitivity to pain and hereditary sensory and autonomic neuropathies affecting peripheral sensory and autonomic neurons. Deregulation of this gene is associated with solid cancers and hematological malignancies, including chronic myeloid leukaemia. PRDM13 6q16.2 4 ‑ ‑ PRDM14 8q13.3 9 ‑ A member of the PRDM family of transcriptional regulators. May possess HMT activity and has a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may have a role in breast cancer. PRDM15 21q22.3 36 ‑ ‑ PRDM16 1p36.32 18 ‑ A zinc finger transcription factor containing an N‑terminal PR domain. Translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may have an important role in the pathogenesis of MDS and AML.

D, Histone demethylase‑encoding and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

JARID2 6p22.3 23 41.22 A DNA‑binding protein that functions as a transcriptional repressor, which interacts with the PRC2 that has an essential role in regulating gene expression during embryonic development. Also facilitates the recruitment of the PRC2 complex to target genes. JMJD1C 10q21.3 30 263.2 Interacts with thyroid hormone receptors and contains a Jumonji domain. A candidate HDM that is thought to be a coactivator for key transcription factors. Participates in the DNA‑damage response pathway by demethylating the mediator of DNA damage checkpoint 1 protein. Mutations in this gene are associated with Rett syndrome and intellectual disability. JMJD4 1q42.13 6 52.5 ‑ JMJD5 16p12.1 10 47.28 A histone lysine demethylase which is a tumor suppressor. MINA 3q11.2 13 53.23 A c‑Myc target gene that may have a role in cell proliferation or regulation of cell growth PHF2 9q22.31 22 62.09 A protein which contains a zinc finger‑like PHD finger, distinct from other classes of zinc finger motifs, and a hydrophobic and highly conserved domain. PHF8 Xp11.22 28 113.93 A histone lysine demethylase, which has an N‑terminal PHD finger and a central Jumonji C domain and preferentially acts on histones in the monomethyl or dimethyl states. Functions as a transcriptional activator. Defects in this gene are a cause of syndromic X‑linked Siderius type intellectual disability and overexpres sion of this gene is associated with several types of cancer. Table SI. Continued.

D, Histone demethylase‑encoding and associated genes

Protein Gene Location Exon mass (kDa) Encoding protein and biological function

UTY Yq11.221 45 17.38 Involved in protein‑protein interactions. Also a minor histocompatibility antigen, which may induce graft rejection of male stem cell grafts.

‘‑’ not reported; HDAC, ; HAT, histone acetyltransferase; HMT, histone methyltransferase; MEF, myocyte enhancer factor; Rb, retinoblastoma; CREB, cAMP‑response element binding protein; MLL, mixed‑lineage leukemia; RTS, Rubinstein‑Taybi syndrome; bHLH, basic helix‑loop‑helix; PRMT, protein arginine N‑methyltransferase; PHD, plant homeodomain; PRC, polycomb repressive complex; ASCOM, ASC‑2/NCOA6 complex; WHS, Wolf‑Hirschhorn syndrome; PRDI, positive regulatory domain I element; KRAB, Kruppel‑associated box; SET, su(var)3‑9, enhancer‑of‑zeste, trithorax; PR domain, PRDI‑BF1 and RIZ homology domain; PRDM, PR/SET domain. MDS, myelodys‑ plastic syndrome; AML, Acute Myelocytic Leukemia. Table SII. Association between NCOA1, ESR1 and ATAD2 expression and clinicopathological parameters in gastric cancer.

NCOA1 ESR1 ATAD2 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ Feature N Low High P‑value Low High P‑value Low High P‑value

Sample type Tumor 375 211 164 <0.001 262 113 <0.001 215 160 <0.001 Non‑tumor 32 8 24 9 23 31 1 Age (years) <60 105 62 43 0.401 69 36 0.088 68 37 0.075 ≥60 229 131 98 172 57 124 105 Sex Male 214 124 90 0.937 159 55 0.205 122 92 0.908 Female 120 69 51 81 39 70 50 Location GEJ 35 24 11 0.432 26 9 0.384 22 13 0.402 Cardia/proximal 43 24 19 30 13 30 13 Fundus/body 119 67 52 81 38 62 57 Stomach body 5 3 2 5 0 2 3 Antrum/distal 127 73 54 94 33 73 54 Other 5 2 3 5 0 3 2 AJCC stage I 46 26 20 0.772 34 12 0.655 24 22 0.514 II 112 67 45 83 29 67 45 III 142 77 65 97 45 83 59 IV 34 23 11 26 8 18 16 T‑stage T1a/b 15 10 5 0.577 13 2 0.480 8 7 0.834 T2 68 37 31 49 19 39 29 T3 158 96 62 115 43 88 70 T4a/b 93 50 43 63 30 57 36 N‑stage N0 105 62 43 0.112 75 30 0.331 63 42 0.759 N1 89 57 32 68 21 47 42 N2 71 42 29 53 18 41 30 N3a/b 69 32 37 44 25 41 28 M‑stage M0 306 174 132 0.319 220 86 1.000 177 129 0.693 M1 28 19 9 20 8 15 13

AJCC, American Joint Committee on Cancer; NCOA1, nuclear receptor coactivator 1; ESR, estrogen receptor; ATAD2, ATPase family AAA domain‑containing protein 2; GEJ, gastroesophageal junction.