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Mai Muuttunut Pillott out On MAIMUUTTUNUT US009925135B2 PILLOTT OUT ON HII (12 ) United States Patent ( 10 ) Patent No. : US 9 ,925 , 135 B2 Fournial et al. (45 ) Date of Patent: Mar. 27 , 2018 ( 54 ) USE OF AN EXTRACT OF PLANT ORIGIN FOREIGN PATENT DOCUMENTS OF GLOBULARIA AND METHOD FOR OBTAINING SAID EXTRACT BY IN VITRO EP 0006059 Al 12 / 1979 PLANT CULTURE FR 2850572 A1 8 / 2004 WO 1994 / 07837 A1 4 / 1994 WO 99 / 13855 A 3 / 1999 ( 75 ) Inventors: Arnaud Fournial, Paris (FR ) ; WO 200043417 A1 7 / 2000 Claire -Marie Grizaud , Plaisir (FR ) ; wo 2002 /066668 A2 8 / 2002 Philippe Mondon , Montrouge (FR ) ; WO 2003 / 068141 A2 8 / 2003 Caroline Le Moigne, Foster City , CA WO 04 / 024695 AL 3 / 2004 WO 2004 / 024695 AL 3 / 2004 (US ) WO 2004 / 037184 A2 5 / 2004 WO 2004 / 101609 A2 11 / 2004 ( 73 ) Assignee : Sederma, S . A . S . (FR ) WO 2005048968 A1 6 / 2005 WO 2005 / 102266 AL 11 / 2005 ( * ) Notice : Subject to any disclaimer, the term of this WO 2006075311 A1 7 / 2006 patent is extended or adjusted under 35 WO 2006120646 AL 11/ 2006 WO 2007029187 A2 3 / 2007 U . S . C . 154 (b ) by 825 days . wo 2010067327 Al 6 / 2010 ( 21 ) Appl. No. : 13 /981 ,837 OTHER PUBLICATIONS (22 ) PCT Filed : Jan . 30 , 2012 Sesterhenn et al . Plant Cell Reports . Mar. 2007 , vol. 26 , Issue 3 , pp . PCT/ IB2012 /050424 365 - 371. * ( 86 ) PCT No. : Miyase et al. J Nat Prod . vol. 62 , No. 8 ( 1999 ) 1079 - 1084 . * $ 371 (c ) ( 1 ) , Chaudhuri , et al. , Globularifolin , a New Acyl Iridoid Glucoside ( 2 ) , ( 4 ) Date : Jul . 25 , 2013 from Globularia cordifolia ; Helvetica Chimica Acta , Jan . 23 , 1980 , 63: 1 , pp . 117 - 120 , XP002659492 . (87 ) PCT Pub . No .: WO2012/ 104774 H . Kirmizibekmez , I. Calis , P . Akbay, 0 . Sticher : “ Iridoid and Bisiridoid Glycosides from Globularia cordifolia ” , Zeitschrift für PCT Pub . Date : Aug . 9 , 2012 Naturforschung C ., Journal of biosciences, vol. 58c , 2003, pp . (65 ) Prior Publication Data 337 - 341, XP002659494 . US 2013 /0309332 A1 Nov. 21, 2013 (Continued ) Related U . S . Application Data Primary Examiner — Amy L Clark (60 ) Provisional application No . 61/ 495 , 020 , filed on Jun . (74 ) Attorney , Agent, or Firm — Lerner, David , 9 , 2011 . Littenberg , Krumholz & Mentlik , LLP ( 30 ) Foreign Application Priority Data Jan . 31, 2011 ( FR ) .. .. 11 50739 (57 ) ABSTRACT (51 ) Int. Ci. According to the present invention the extract of plant origin A61K 8 / 97 ( 2017 . 01 ) of the Globularia genus is used for a non - therapeutic A61K 8 /99 ( 2017 . 01 ) cosmetic treatment of the skin and/ or appendages . Prefer A6IK 36 / 185 ( 2006 . 01 ) ably the species is Globularia cordifolia and the extract is A61Q 19 / 08 ( 2006 . 01) obtained by in vitro plant culture. The extract can be used in A610 5 /00 ( 2006 .01 ) particular for preventing and /or treating skin ageing by A610 19 / 00 ( 2006 .01 ) stimulating the 10 reactions of detoxification and cellular A61Q 7 / 00 ( 2006 .01 ) regeneration through a hormetic type response , for improv ( 52 ) U .S . CI. ing the transparency and radiance of complexion , for pre CPC . A61K 8 / 97 (2013 .01 ) ; A61K 8 / 99 venting or treating sensitive and reactive skins, for prevent ( 2013 . 01 ) ; A61K 36 / 185 ( 2013 .01 ) ; A610 ing or treating rednesses , for preventing protein glycation , 5 /002 (2013 . 01 ) ; A61Q 19 / 00 ( 2013 .01 ) ; for increasing and /or maintaining the number of dermal stem A61Q 19 / 08 (2013 .01 ) ; A61K 2800 /522 cells , for increasing and / or maintaining the dermal macro ( 2013 . 01 ) ; A61Q 7 /00 (2013 .01 ) ; A61Q 19 / 005 molecules , in particular collagen and elastin , for increasing ( 2013. 01 ) the volume of the dermis , for 15 preventing and / or treating ( 58 ) Field of Classification Search fines lines and wrinkles , for firming skin , for preventing hair None loss and /or stimulating hair regrowth . The invention also See application file for complete search history . proposes an original method of obtaining the extract by in vitro plant culture of undifferenciated cells of Globularia ( 56 ) References Cited and an extract of plant origin that can be obtained by such U . S . PATENT DOCUMENTS method . 2003/ 0082117 Al 5 / 2003 Martin et al. 2008 / 0003308 A1 1 / 2008 Giuliani et al. 5 Claims , No Drawings US 9 ,925 , 135 B2 Page 2 ( 56 ) References Cited OTHER PUBLICATIONS H . Kirmizibekmez , I. Calis , S . Piacente , C . Pizza : “ Phenolic com pounds from Globularia cordifolia ” , Turk J Chem , vol . 28 , 2004 , pp . 455 - 460 , XP002659493 . Harborne et al . ; 6 -Hydroxyluteolin and Scutellarein as Phyletic Markers in Higher Plants ; Phytochemistry , Feb . 1971 ( Feb . 1971 ) , 10 : 2 , pp . 367 -378 , XP002659491. International Search Report for Application No . PCT/ IB2012 / 050424 dated Jul. 17 , 2012 . * cited by examiner US 9 ,925 , 135 B2 USE OF AN EXTRACT OF PLANT ORIGIN 02, p . 367 - 3 ' 78 ; “ Globularifolin , a new acyl iridoid gluco OF GLOBULARIA AND METHOD FOR side from Globularia cordifolia " , Helvetica Chimica Acta , OBTAINING SAID EXTRACT BY IN VITRO vol . 63 , no . 1 , Jan . 23 , 1980 , p . 117 -120 ; “ Phenolic com PLANT CULTURE pounds from Globularia cordifolia ” , Turk J Chem , vol. 28 , 5 2004 , p . 455 - 460 and “ Iridoid and bisiridoid glycosides from CROSS -REFERENCE TO RELATED Globularia cordifolia ” Journal of biosciences, vol. 58c . , APPLICATIONS 2003 , p . 337 - 341 ) . The present application is a national phase entry under 35 SUMMARY OF THE INVENTION U . S . C . $ 371 of International Application No. PCT / IB2012 / 10 050424 filed Jan . 30 , 2012 , published in English , which The aim of the present invention is to provide a new claims priority from French Application No . FR1150739 extract of plant origin for the cosmetic industry always in filed Jan . 31 , 2011 and U . S . Application 61/ 495 ,020 filed demand of new active ingredients . Jun . 9 , 2011 , all of which are incorporated herein by To this aim , the present invention proposes the use of an reference . 15 extract of plant origin of the Globularia genus for a non therapeutic cosmetic treatment of the skin and /or append TECHNICAL FIELD ages. The skin is subject to many external stresses : UV, elec The subject matter of the present invention is a new use tromagnetic waves , oxidants , pollutants , biological agents , of an extract of plant origin of Globularia and a preferred 20 etc . These stresses induce, among other deleterious effects , method of obtaining said extract by in vitro plant culture . micro - inflammatory phenomena that are more or less The present invention concerns more generally the cos chronic and the formation of toxic products in epidermal metics industry, that produce and / or use extracts of plant cells or at their periphery . origin for preventing of treating disorders of skin , scalp , Among these products are the pre - inflammatory media mucous and appendages ( such as hair , eyelashes, eyebrows, 25 tors and some growth factors that cause a local over nails or hairs ) of animal or human mammals . densification of epidermal nerve endings associated with a According to the invention , " extract of plant origin ” hyper- reactivity to external stimuli . This results in lowering means an extract that can be obtained directly from a plant the threshold of the skin regarding these stimuli . It becomes or that can be obtained by culture of plant cells or tissues . very sensitive , generating discomfort but also most of the 30 time transient rednesses . BACKGROUND ART In addition , the skin gradually accumulates debris of various origins, composed of proteins , carbohydrates and In cosmetics , there are many ways to improve the overall altered lipids. These components (carbonylated proteins, condition of the skin , hair and nails including imparting or lipoperoxides, lipofuscin , etc . ) will also act as toxins that restoring skin tone , hydration , pigmentation or depigmen - 35 disrupt the physiology and cellular homeostasis and modify tation , protection against external aggressions such as UV fragile metabolic balances . radiations or coldness, calming irritation , redness , acne , Over time, they alter mitochondrial respiration , mem reducing micro -oedemas (such as bags under the eyes ) , brane integrity , enzymatic activity , and detoxification reducing dark circles, signs of aging such as wrinkles, fine mechanisms such as the proteasome. Such changes can lines , pigmentation , restoring suppleness and elasticity , 40 cause changes in behavior of certain cells such as stem cells . treating hair loss , acting on the adipose tissues , adding As a matter of fact , the latter are in a particular environment volume , density , improving the texture etc . in which the change may lead to the weakening of their Since a long time and in all parts of the world , the plant character and their progenitor phenotype ( cell size larger , kingdom is an abundant and widely used source of biologi - reduction of the multipliers capacity etc . ) , making them less cally active substances in particular in the pharmaceutical 45 able to hold their role in the renewal of keratinocytes . and cosmetics fields . Experimental data show that mild stress received in an Globularia genus belongs to the Plantaginaceae family , early stage can delay degradation and therefore skin aging , Globularioideae subfamily , and comprises several species, helping the body to later deal with more severe stress including those known: Globularia alypum , Globularia bis - (external events ) . This phenomenon is called hormesis . It is nagarica , Globularia cordifolia , Globularia nudicaulis , 50 based on the activation of detoxification mechanisms and Globularia vulgaris , Globularia gracilis , Globularia repens cellular defense in response to a mild stress or in response and Globularia valentina . to substances which are said to have a hormetic effect They are small herbaceous plants , with leaves simple , (molecules called hormetins ) . The hormesis will contribute alternate , with flowers in round heads , from subtropical to to a better adaptation of the cell and thus of the body with temperate regions . They are found mainly in Europe and 55 regards to subsequent stresses, allowing it to fight more North Africa .
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