DOCSLIB.ORG

The Critical Review of Methodologies and Approaches to Assess the Inherent Skin Sensitization Potential (Skin Allergies) of Chemicals No 2009 61 04

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Critical Review of Methodologies and Approaches to Assess the Inherent Skin Sensitization Potential (Skin Allergies) of Chemicals No 2009 61 04 The Critical Review of Methodologies and Approaches to Assess the Inherent Skin Sensitization Potential (skin allergies) of Chemicals No 2009 61 04 Executive Agency for Health and Consumers 1 Purpose and context of contract Aims The objective of the contract is to increase the knowledge base for a systematic approach to the issue of skin allergies by conducting the following work: 1. Critically review currently available methods, or methods under development ( in vivo , in vitro , in silico , etc.) used in the evaluation of skin sensitization potential and their applicability in the derivation of quantitative “safety thresholds”. 2. Identify specific cases, classes or specific use situations of chemicals for which “safety thresholds” or “safety limits” were set (in regulations, standards, in scientific research/clinical work, etc.) and critically review the scientific and methodological parameters used to set those limits. 3. For those chemicals identified in point 2 above, collect and critically analyse clinical and statistical evidence on the incidence and morbidity (clinical picture) of skin contact allergies (contact dermatitis) cases in the European Union before (at least 3 years) and after the limits were set so as to allow an assessment of the possible effect of the limits in the reduction/prevention of the incidence and morbidity of contact dermatitis. 2 Abbreviations IPPD N-isopropyl-N'-phenyl-p- AEL Acceptable exposure level phenylenediamine APC Antigen presenting cell ISO International Organization for CASE Computer automated structure Standardization evolution program ITS Integrated Testing Strategy CCET Cumulative contact enhancement IVDK Information Network of test Departments of Dermatology CEL Consumer exposure level LLNA Local lymph node assay CEN Comité Européen de MAK Maximale Arbeitsplatz Normalisation (European Konzentration (threshold limit Committee for Standardization) value) DCDG Danish Contact Dermatitis Group MEC Minimal elicitation concentration DfW Derek for Windows expert system MEST Mouse ear swelling test DPRA Direct peptide reactivity assay MCI/MI Methylchloroisothiazolinone/ DMG Dimethylglyoxime methylisothiazolinone DST Dermal sensitization threshold MDBGN Methyldibromoglutaronitrile ECETOC European Centre for MUST Myeloid U937 skin sensitization Ecotoxicology and Toxicology of test Chemicals NESIL No expected sensitization ECVAM European Centre for Validation of induction level Alternative Methods NOEL No effect level ED Efficient dose PPD p-Phenylenediamine EECRDG European Environmental Contact QMM Quantitative mechanistic models Dermatitis Research Group QRA Quantitative risk assessment EINECS European Inventory of Existing QSAR Quantitative structure activity Commercial Chemical Substances relationship ELINCS European list of notified chemical R43 Designates “sensitizing to the substances skin” EN Norme Européen (European RAI Relative alkylation index standard maintained by CEN) ROAT Repeated open application test ESCD European Society of Contact REACH Registration, Evaluation, Dermatitis Authorisation and Restriction of ESSCA European Surveillance System on Chemicals Contact Allergies SAF Safety assessment factor EU European Union SAR Structure activity relationship FM Fragrance mix SCCP Scientific Committee on Consumer GPMT Guinea pig maximization test Products GSH Glutathione TIMES-SS Tissue metabolism simulator for HBV Hepatitis B virus skin sensitization h-CLAT Human cell activation test TOPKAT Toxicity prediction komputer- HICC Hydroxyisohexyl 3-cyclohexene assisted technology carboxaldehyde TTC Threshold of toxicological concern HIV Human immunodeficiency virus TRGS Technische Regel Gefahrstoffe HRIPT Human repeated insult patch test (Technical regulations for hazardous IFRA International Fragrance substances) Association TRUE-test Thin-layer Rapid Use INCI International Nomenclature of Epicutaneous Test Cosmetic Ingredients UK United Kingdom WoE Weight of evidence 3 Participants Chairman Professor Torkil Menné Chair of the Department of Dermato-Allergology Gentofte Hospital Niels Andersens Vej 65 DK-2900 Hellerup Denmark Jacob Pontoppidan Thyssen, MD PhD Department of Dermato-Allergology Gentofte Hospital Niels Andersens Vej 65 DK-2900 Hellerup Denmark Partner 1 Professor Anders Boman Unit of Occupational and Environmental Dermatology Karolinska Institute Norrbacka SE-171 76 Stockholm Sweden Partner 2 Professor Jean-Pierre Lepoittevin Laboratoire de Dermatochimie Institut le Bel 4 Rue Blaise Pascal CS 90032 F-67081 Strasbourg Cedex France Elena Giménez-Arnau, DMSci Laboratoire de Dermatochimie Institut le Bel 4 Rue Blaise Pascal CS 90032 F-67081 Strasbourg Cedex France Partner 3 Professor Axel Schnuch IVDK-Zentrale Institut an der Universität Göttingen von Sieboldstr. 3 D-37075 Goettingen Germany 4 Executive summary Contact sensitization is caused by a group of reactive chemicals referred to as allergens, mostly man-made, that are able to permanently change a subgroup of immune cells so that they will proliferate and target the skin compartment upon allergen re-exposure. Reactive chemicals and metals are present in the domestic and the occupational environment. Natural occurring contact sensitizing chemicals or substances are known but represent a very limited clinical problem. The induction of allergen specific memory T-cells is a frequent happening since up to 20% of European adults are contact sensitized. The allergen specific T-cells will in all foreseeable future recognise the chemical in question and react with an inflammatory response upon re- exposure, defined as elicitation and clinically expressed as allergic contact dermatitis. Individuals who are contact sensitized can be identified by a diagnostic test referred to as the patch test. The patch test mimics biological re-exposure since an individual is exposed to small amounts of chemicals applied in small metallic chambers directly on the skin, performed under internationally standardized conditions. The most common contact sensitizing chemicals include metals such as nickel and chromium, preservatives, fragrances and hair dye chemicals. Approximately 25 chemicals are currently included in the European baseline patch test series used by dermatologists; a test series that include chemicals where the frequency of positive test reactions exceed 1%. In studies of random samples from the general population, 10-15% of children and 15–25% of adults have a positive patch test reaction to one or more of these chemicals and are therefore contact allergic. A total of 4 000 chemicals are known to have a contact sensitizing potential and approximately 100 are regularly encountered with positive patch test reactions in dermatology practice. Allergic contact dermatitis appears primarily at areas of skin contact. It is therefore not surprising that dermatitis is mainly located on the hands and face, skin areas that are in contact with e.g. cosmetics, and jewellery. The clinical picture may include redness, oedema, scaling, fissures and in the acute phase vesicles, bullae and eventually oozing. Secondary infection might be present as well. Figure 1-4 illustrate the variability in the morphology of the disease. Patients with dermatitis need to undergo diagnostic evaluation and treatment. If a single causative allergen can be identified and future contact avoided, dermatitis will typically disappear within 2–3 months. However, hand dermatitis tends to have a more chronic course with 50% of patients having persistent or intermittent symptoms. Contact sensitization and related skin diseases (allergic contact dermatitis at site of exposure and hand dermatitis) may severely affect an individual’s quality of life and working capabilities. It is difficult to quantify national expenses associated with contact sensitization but they are high. The contact sensitization problem has mainly been caused by human engineered chemicals introduced into consumer and occupational products over the last 100 years. Knowledge obtained from observing contact sensitization epidemics over the 20 th century provides the basis for future prevention of contact sensitization and related disease. In general, genetic predisposition plays a very little role for contact sensitization as the condition is mainly caused by environmental exposure. The decisive factors include the inherent sensitizing potential of a chemical, skin exposure concentration and the number of exposures. The present report describes and discusses available methods to identify the potency of contact sensitizing chemicals and to understand the dose-response effects in humans and the effect of regulation of some of these important chemicals. The report is divided into 3 parts. Part 1 describes methods that are available to determine whether a chemical holds a contact sensitizing potential. Some methods have been in use for more than 50 years, others still undergo final standardisation. In vitro tests include binding assays between chemicals and standard receptor molecules based on benchmarking with chemicals known not to be sensitizing. In silico tests represent computer identifications of chemical structures known to be present in contact sensitizing chemicals. A new group of methods, based on cultured human cells, might to some extend replace traditional animal testing based on mice or guinea 5 pigs as the latter after 2013 will be outsourced due to the REACH agreement. The most standardised method used today, to establish the sensitizing potency of chemicals, is the local lymph node assay
Load more

Recommended publications
  • Brucellosis in Humans – Etiology, Diagnostics, Clinical Forms
    Annals of Agricultural and Environmental Medicine 2013, Vol 20, No 2, 233–238 REVIEW ARTICLE www.aaem.pl Brucellosis in humans – etiology, diagnostics, clinical forms Elżbieta Monika Galińska1, Jerzy Zagórski2 1 Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland 2 Department of Public Health, Institute of Rural Health, Lublin, Poland Galińska EM, Zagórski J. Brucellosis in humans – etiology, diagnostics, clinical forms. Ann Agric Environ Med. 2013; 20(2): 233–238. Abstract Brucellosis in humans is a zoonosis of greatly varied clinical image. It occurs on all inhabited continents. The course of the disease may be acute, sub-acute or chronic. The etiologic factors of brucellosis are small, aerobic Gram-negative rods of the genus Brucella, which currently contains ten species: B. abortus, B. suis, B. ovis, B. melitensis, B. canis, B. neotomae, B. pinnipedialis, B. ceti, B. microti and B. inopinata. In humans, the disease is caused mainly by: B. melitensis as the most pathogenic species, followed by B. suis, whereas B. abortus is considered as the mildest type of brucellosis. The natural reservoir of the germ and the source of infection in humans are infected domestic animals, primarily cattle, sheep, goats, as well as wild animals. Infection in humans occurs by penetration through damaged skin, conjunctiva, and more rarely via the alimentary route by the consumption of infected products. Especially exposed are: veterinarians, veterinary technicians, insemination service employees, zoo technicians,
    [Show full text]
  • (DNCB) and Respiratory Allergy in the Th2-Prone Brown Norway Rat C
    Available online at www.sciencedirect.com Toxicology 246 (2008) 213–221 The contact allergen dinitrochlorobenzene (DNCB) and respiratory allergy in the Th2-prone Brown Norway rat C. Frieke Kuper a,∗, Rob H. Stierum a, Andre Boorsma a,b, Marcel A. Schijf a, Menk Prinsen a, Joost P. Bruijntjes a, Nanne Bloksma c, Josje H.E. Arts a a TNO, Quality of Life, Zeist, The Netherlands b Department of Health Risk Analysis and Toxicology, Maastricht University, Maastricht, The Netherlands c Subfaculty of Biology, Faculty of Science, Utrecht University, The Netherlands Received 30 October 2007; received in revised form 21 December 2007; accepted 21 January 2008 Available online 2 February 2008 Abstract All LMW respiratory allergens known to date can also induce skin allergy in test animals. The question here was if in turn skin allergens can induce allergy in the respiratory tract. Respiratory allergy was tested in Th2-prone Brown Norway (BN) rats by dermal sensitization with the contact allergen dinitrochlorobenzene (DNCB; 1%, day 0; 0.5%, day 7) and a head/nose-only inhalation challenge of 27 mg/m3 of DNCB (15 min, day 21), using a protocol that successfully identified chemical respiratory allergens. Skin allergy to DNCB was examined in BN rats and Th1-prone Wistar rats in a local lymph node assay followed by a topical patch challenge of 0.1% DNCB. Sensitization of BN rats via the skin induced DNCB-specific IgG in serum, but not in all animals, and an increased number of CD4+ cells in the lung parenchyma. Subsequent inhalation challenge with DNCB did not provoke apneas or allergic inflammation (signs of respiratory allergy) in the BN rats.
    [Show full text]
  • (Trouessart) and D. Farinae Hughes (Acari: Pyroglyphidae) Sensitivity in Patients with Allergic Rhinitis: a Comparative Study
    Systematic & Applied Acarology 23(2): 404–404 (2018) ISSN 1362-1971 (print); http://dx.doi.org/10.11158/saa.23.2.17 ISSN 2056-6069 (online) Erratum Correction: Evaluation of Dermatophagoides pteronyssinus (Trouessart) and D. farinae Hughes (Acari: Pyroglyphidae) sensitivity in patients with allergic rhinitis: a comparative study ERHAN ZEYTUN1*, SALİH DOĞAN1, EDHEM ÜNVER2 & FATİH ÖZÇİÇEK3 1 Department of Biology, Arts & Sciences Faculty, Erzincan University, Erzincan, Turkey 2 Department of Chest Disease, Erzincan University School of Medicine, Erzincan, Turkey 3 Department of Internal Medicine, Erzincan University School of Medicine, Erzincan, Turkey * Corresponding author: [email protected] The authors are sorry that they made a mistake in spelling the family name of the first author on line 4 of page 206 in Zeytun et al. (2018): EYTUN should be ZEYTUN. This type error is evident in the paper itself because the running title in the footer of page 207 has the correct name: “ZEYTUN ET AL.: SENSITIVITY TO HDMS IN PATIENTS WITH ALLERGIC RHINITIS”. Reference Zeytun1, E., Doğan, S., Ünver, E. & Özçiçek, F. (2018) Evaluation of Dermatophagoides pteronys- sinus (Trouessart) and D. farinae Hughes (Acari: Pyroglyphidae) sensitivity in patients with allergic rhinitis: a comparative study. Systematic & Applied Acarology, 23(2), 206–215. https://doi.org/10.11158/saa.23.2.2 Published: 22 Feb. 2018 1. Not “Eytun” 404 © Systematic & Applied Acarology Society Systematic & Applied Acarology 23(2): 206–215 (2018) ISSN 1362-1971 (print) http://doi.org/10.11158/saa.23.2.2
    [Show full text]
  • Culicoides Obsoletus Allergens for Diagnosis of Insect Bite Hypersensitivity in Horses
    Culicoides obsoletus allergens for diagnosis of insect bite hypersensitivity in horses Nathalie M.A. van der Meide Thesis committee Promotor Prof. dr. ir. H. F. J. Savelkoul Professor of Cell Biology and Immunology Wageningen University Co-promotor Dr. E. Tijhaar Assistent professor, Cell Biology and Immunology Group Wageningen University Other members Prof. dr. ir. B. Kemp, Wageningen University, The Netherlands Dr. B. Wagner, Cornell University, Itaca, USA Prof. dr. V.P.M.G. Rutten, Utrecht University, The Netherlands Prof. dr. R. Gerth van Wijk, Erasmus MC Rotterdam, The Netherlands This research was conducted under the auspices of the Graduate School of the Wageningen Institute of Animal Sciences Culicoides obsoletus allergens for diagnosis of insect bite hypersensitivity in horses Nathalie M.A. van der Meide Thesis Submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. dr. M. J. Kropff, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Friday 13 September 2013 at 1.30 p.m. in the Aula Nathalie M.A. van der Meide Culicoides obsoletus allergens for diagnosis of insect bite hypersensitivity in horses PhD Thesis, Wageningen University, Wageningen, NL (2013) With references, with summaries in Dutch and English ISBN: 978-94-6173-669-7 Contents Chapter 1 General introduction 7 Chapter 2 Culicoides obsoletus extract relevant for diagnostics of insect bite hypersensitivity in horses 45 Chapter 3 Seasonal
    [Show full text]
  • Cscl Specimen Collection Guide 1
    CSCL SPECIMEN COLLECTION GUIDE 1 Test Name Alternative Name Specimen Special Requirements ? CSF β-transferrin, tau transferrin Nasal or ear secretions ?Parasite Parasitosis Parasite in a pottle 1,25 dihydroxy cholecalciferol 1,25 diOH Vit D, 1,25 dihydroxy Vit D, Calcitriol Yellow Protect from light with tinfoil. 1,25 Di-OH Vit D 1,25 Dihydroxy Vitamin D, 1,25 diOH cholecalciferol, Calcitriol Yellow Protect from light with tinfoil. 11 Deoxycortisol Metyrapone test Yellow Take dedicated tube. State whether pre or post metyparone sample. 17 hydroxysteroids 17 ketosteroids, Urine cortisol Urine - random or 24hr plain collection 17 β estradiol - sensitive Yellow Preferred sample collected between 0800-1000. 17-Ketosteroid - refer to Cortisol 17OH Progesterone Yellow Note pre and post dose on CHL sticker if this test is done with a synacthen test. 17-OH Progesterone/P4 17α hydroxy progesterone Yellow Note pre and post dose on CHL sticker if this test is done with a synacthen test. 17α hydroxy progesterone 17-OH progesterone/P4 Yellow Note pre and post dose on CHL sticker if this test is done with a synacthen test. 17β Oestradiol Estradiol, Estrogen, E2, Oestradiol Yellow 17β Oestradiol - Sensitive Estradiol, Estrogen, E2, Oestradiol Yellow NOTE: Sensitive assay no longer available at CHL. OES done at CSCL instead. 25-hydroxy cholecalciferol Vitamin D, Cholecalciferol, 25-OH vitamin D Yellow 25-hydroxy vitamin D 25-OH vitamin D, cholecalciferol Yellow 25-OH cholecalciferol 25-hydroxy vitamin D Yellow 5 hydoxy tryptamine - see 5HT 5HIAA HIAA Quantitation, 5-hydroxy indole acetic acid, serotonin metabolites, 24hr Urine bottle (acid or plain) or random collection (>5mL) Medication history must be noted on request form.
    [Show full text]
  • Skin Allergy Testing
    CONSENT WHAT TO EXPECT AT WELLINGTON SCL Allergens used in Skin Prick Testing, including the positive Our experienced staff are fully trained in the performance of these control are considered to be medicines therefore required tests but the ordering doctor remains responsible for explaining the to be registered in New Zealand. Unfortunately the results. Histamine control and some allergens available are not currently registered, therefore come under Section 29 of Once your arm (or back) is prepared a small amount of each allergen the Medicines Act. is applied and pricked. Your immune system will interact with the allergen over the next 15 minutes and produce an itchy wheal (like Under the Medicines Act 1981, the pharmaceutical a mosquito bite) if you have sensitisation to that product. You must company selling the medicine has to inform the Director not scratch the wheals while they develop as this may change their Skin Allergy of Health that it has sold the medicine to the laboratory, intensity. and has to name the doctor and yourself, describe the medicines and say where and when the medicine was sold. Some medications, such as antihistamines and antidepressants may This means we may have to supply these particulars to the interfere with the results. Please let us know what medications are Testing pharmaceutical company. being taken before booking the appointment. Negative testing does not guarantee there is no allergy but makes an allergic cause less likely. Further tests may be required to find I the cause of the problem or challenge testing may be required. Our have received enough information to provide my consent Immunopathologists also work as Allergists and welcome contact for the performance of the Skin Allergy Test.
    [Show full text]
  • Opinion on Allergy Alert Test (AAT) As a Proof-Of-Concept Study ______
    SCCS/1607/19 Final Opinion Scientific Committee on Consumer Safety SCCS OPINION ON Allergy Alert Test (AAT) as a proof-of-concept study The SCCS adopted the final Opinion by written procedure on 10 September 2019 SCCS/1607/19 Final Opinion Opinion on Allergy Alert Test (AAT) as a proof-of-concept study _________________________________________________________________________________________________________________ ACKNOWLEDGMENTS SCCS members listed below are acknowledged for their valuable contribution to the finalisation of this Opinion. For the preliminary and the final versions SCCS members Dr U. Bernauer Dr L. Bodin Prof. Q. Chaudhry (SCCS Chair) Prof. M. Dusinska Dr J. Ezendam Dr E. Gaffet Prof. C. L. Galli Dr B. Granum Prof. E. Panteri Prof. V. Rogiers (SCCS Vice-Chair) Dr Ch. Rousselle Dr M. Stepnik Prof. T. Vanhaecke Dr S. Wijnhoven SCCS external experts Dr A. Koutsodimou Dr A. Simonnard Prof. W. Uter (Rapporteur) All Declarations of Working Group members are available on the following webpage: http://ec.europa.eu/health/scientific_committees/experts/declarations/sccs_en.htm This Opinion has been subject to a commenting period of a minimum eight weeks after its initial publication (from 13 May 2019 until 12 July 2019). Comments received during this time period were considered by the SCCS. For this Opinion, comments received resulted in the following main changes: SCCS comments in sections 3.2.4, 3.3.2.1. and 3.3.2.5. These changes are not impacting the discussion and conclusion parts that remain unchanged. 2 SCCS/1607/19 Final Opinion Opinion on Allergy Alert Test (AAT) as a proof-of-concept study _________________________________________________________________________________________________________________ 1.
    [Show full text]
  • Skin Allergy Test Information for Patients, Relatives and Carers
    Lung Function Laboratory Skin allergy test Information for patients, relatives and carers This leaflet has been designed to give you information about skin allergy test and answers some of the questions that you or those who care for you may have. It is not meant to replace the discussion between you and your medical team but aims to help you understand more about what is discussed. If you have any questions about the information below, please contact us. Introduction The skin allergy test or skin prick test is a diagnostic test that investigates the immediate response of your body when exposed to specific allergens. This can aid your doctor in diagnosing and treating the symptoms you may be suffering from. What to expect on the day The test will be performed by putting drops of liquid allergen on the skin of your forearm and then lightly pricking the skin to see if a reaction occurs. Once the reactions have had a sufficient time to develop they will then be measured with the entire process taking roughly 30 minute in duration. During this time you may experience some minor swelling and itchiness around the test sites which should subside prior to you leaving the lung function department. Allergens we test for Air-borne allergens are transported through the air and into the nose, which can cause an allergic reaction. We test the most common allergy triggers from within and around your home environment. These include animal fur, feathers, tree and flower pollen, mould spores and house dust mite. We test 10 allergens as well as two control solutions.
    [Show full text]
  • Sensitivity to House Dust Mites Allergens in Patients with Allergic
    34 Original Investigation / Özgün Araştırma Sensitivity to House Dust Mites Allergens in Patients with Allergic Asthma in Erzincan Province, Turkey Erzincan’da Alerjik Astımlı Hastaların Ev Tozu Akar Alerjenlerine Karşı Duyarlılığı Erhan Zeytun1, Salih Doğan1, Fatih Özçiçek2, Edhem Ünver3 1Department of Biology, Erzincan University Faculty of Arts and Sciences, Erzincan, Turkey 2Department of Internal Medicine, Erzincan University School of Medicine, Erzincan, Turkey 3Department of Chest Disease, Erzincan University School of Medicine, Erzincan, Turkey ABSTRACT Objective: To investigate the sensitivity of allergic asthma (AA) patients to house dust mites (HDM) by conducting skin tests, measuring total and specific IgE antibodies toDermatophagoides pteronyssinus and D. farinae mites, and examining HDM fauna in patients’ homes. Methods: The study included 25 patients with AA and 31 healthy controls, who were challenged with Der p and Der f allergens; serum levels of allergen-specific lgE and total IgE were measured. Dust samples were collected from the homes of all participants, and mite species and the number of mites per gram of dust were investigated. Results: D. pteronyssinus was found in the homes of 94.7% patients with positive Der p reactions in the skin test (p<0.001). D. farinae was found in the homes of 22.2% patients with positive Der f reactions in the skin test (p>0.05). D. pteronyssinus-specific IgE was detected in 75% patients in whose homes D. pteronyssinus was also found, while D. farinae-specific IgE was detected in 16.6% patients in whose homesD. farinae was also found. Conclusion: A part of AA patients residing in Erzincan are sensitive to HDM allergens, and high numbers of mites leading to allergic sensi- tization are found in their homes.
    [Show full text]
  • Atopic Dermatitis with Possible Polysensitization and Monkey Esophagus Reactivity
    www.najms.org North American Journal of Medical Sciences 2010 July, Volume 2. No. 7. Case Report OPEN ACCESS Atopic dermatitis with possible polysensitization and monkey esophagus reactivity Ana Maria Abreu Velez, MD, Ph.D1, Michael S. Howard, MD,1 Bruce R. Smoller MD 2 1Georgia Dermatopathology Associates, Atlanta, Georgia, USA. 2Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA. Citation: Abreu Velez AM, Howard MS, Smoller BR. Atopic dermatitis with possible polysensitization and monkey esophagus reactivity. North Am J Med Sci 2010; 2: 336-340. Availability: www.najms.org ISSN: 1947 – 2714 Abstract Context: Atopic dermatitis is a chronic inflammatory skin disease resulting from interactions between environmental and genetic factors. Recent studies link atopic dermatitis with asthma and with eosinophilic esophagitis. Case Report: Based on this association, we investigated by indirect immunofluorescence the immunoreactivity patterns on monkey esophagus substrate utilizing the serum of a patient with severe atopic dermatitis. We also examined the patient’s skin biopsy by H&E histology and immunohistochemistry. We detected strong deposits of albumin, IgE, IgG, IgD, IgA, Complement/C1q and mast cell tryptase in multiples structures of the skin, as well as a broad pattern of intraepithelial staining on monkey esophagus. Strong staining positivity was also detected within the inflammatory infiltrate around the upper dermal vessels, as well as additional positive staining for the human leukocyte antigen system antigens DR DP and DQ. Conclusion: Our findings demonstrate that there could be an indication for testing patients with severe atopic dermatitis for autoreactivity to filaggrin (anti-keratin antibodies) utilizing monkey esophagus.
    [Show full text]
  • Medical Review
    Publishing Sector of the Society of Physicians of Vojvodina of the Medical Society of Serbia, Novi Sad, Vase Stajica 9 MEDICAL REVIEW JOURNAL OF THE SOCIETY OF PHYSICIANS OF VOJVODINA OF THE MEDICAL SOCIETY OF SERBIA THE FIRST ISSUE WAS PUBLISHED IN 1948 Editor-in-Chief LJILJA MIJATOV UKROPINA Assistant to the Editor-in-Chief for Clinical Branches: PETAR SLANKAMENAC Assistant to the Editor-in-Chief for Imaging Methods: VIKTOR TILL Assistants to the Editor-in-Chief SONJA LUKAČ ŽELJKO ŽIVANOVIĆ EDITORIAL BOARD OKAN AKHAN, Ankara MIROSLAV MILANKOV, Novi Sad ANDREJ ALEKSANDROV, Birmingham OLGICA MILANKOV, Novi Sad STOJANKA ALEKSIĆ, Hamburg IGOR MITIĆ, Novi Sad VLADO ANTONIĆ, Baltimor NADA NAUMOVIĆ, Novi Sad ITZHAK AVITAL, Bethesda AVIRAM NISSAN, Ein Karem KAREN BELKIĆ, Stockholm JANKO PASTERNAK, Novi Sad JEAN-PAUL BEREGI, Lille Cedex ĐORĐE PETROVIĆ, Novi Sad HELENA BERGER, Ljubljana LJUBOMIR PETROVIĆ, Novi Sad KSENIJA BOŠKOVIĆ, Novi Sad TOMISLAV PETROVIĆ, Novi Sad VLADIMIR ČANADANOVIĆ, Novi Sad MIHAEL PODVINEC, Basel IVAN DAMJANOV, Kansas City JOVAN RAJS, Danderyd JADRANKA DEJANOVIĆ, Novi Sad TATJANA REDŽEK MUDRINIĆ, Novi Sad OMER DEVAJA, Meidstone PETAR E. SCHWARTZ, New Haven RADOSLAVA DODER, Novi Sad MILAN SIMATOVIĆ, Banja Luka PETAR DRVIŠ, Split TOMAŠ SKRIČKA, Brno ZORAN GOJKOVIĆ, Novi Sad PETAR SLANKAMENAC, Novi Sad IRENA HOČEVAR BOLTEŽAR, Ljubljana EDITA STOKIĆ, Novi Sad DEJAN IVANOV, Novi Sad ALEXANDER STOJADINOVIĆ, Glen Alen MARIJA JEVTIĆ, Novi Sad MILANKA TATIĆ, Novi Sad MARINA JOVANOVIĆ, Novi Sad VIKTOR TILL, Novi Sad ZORAN KOMAZEC,
    [Show full text]
  • Asia Pacific Educational & Teaching Material Doi: 10.5415/Apallergy.2011.1.1.43 Allergy Asia Pac Allergy 2011;1:43-49
    pISSN 2233-8276 · eISSN 2233-8268 Asia Pacific Educational & Teaching Material doi: 10.5415/apallergy.2011.1.1.43 allergy Asia Pac Allergy 2011;1:43-49 Hypersensitivity manifestations to the fruit mango Richa Sareen and Ashok Shah* Department of Respiratory Medicine, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110 007, India The objectives of this study are 1) To review the published data and document the current knowledge on allergic manifestations to the fruit mango 2) To highlight the two distinct clinical presentations of hypersensitivity reactions caused by mango 3) To discuss the role of cross-reactivity 4) To increase awareness of potentially life threatening complications that can be caused by allergy to mango. An extensive search of the literature was performed in Medline/PubMed with the key terms “mango”, “anaphylaxis”, “contact dermatitis”, “cross-reactivity”, “food hypersensitivity”, “oral allergy syndrome” and “urticaria”. The bibliographies of all papers thus located were searched for further relevant articles. A total of 17 reports describing 22 patients were documented, including ten patients with immediate hypersensitivity reaction and twelve patients with delayed hypersensitivity reaction to mango. Ten of these patients (four with immediate reaction; six with delayed reaction) were from geographical areas cultivating mango, whereas twelve patients (six with immediate reaction; six with delayed reaction) were from the countries where large scale mango cultivation does not occur. The clinical features, pathogenesis and diagnostic modalities of both these presentations are highlighted. The fruit mango can cause immediate and delayed hypersensitivity reactions, as also “oral allergy syndrome”. Although rare, it can even result in a life threatening event.
    [Show full text]