9260 DETECTION of PATHOGENIC BACTERIA* 9260 A. Introduction
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The Role of Earthworm Gut-Associated Microorganisms in the Fate of Prions in Soil
THE ROLE OF EARTHWORM GUT-ASSOCIATED MICROORGANISMS IN THE FATE OF PRIONS IN SOIL Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Taras Jur’evič Nechitaylo aus Krasnodar, Russland 2 Acknowledgement I would like to thank Prof. Dr. Kenneth N. Timmis for his guidance in the work and help. I thank Peter N. Golyshin for patience and strong support on this way. Many thanks to my other colleagues, which also taught me and made the life in the lab and studies easy: Manuel Ferrer, Alex Neef, Angelika Arnscheidt, Olga Golyshina, Tanja Chernikova, Christoph Gertler, Agnes Waliczek, Britta Scheithauer, Julia Sabirova, Oleg Kotsurbenko, and other wonderful labmates. I am also grateful to Michail Yakimov and Vitor Martins dos Santos for useful discussions and suggestions. I am very obliged to my family: my parents and my brother, my parents on low and of course to my wife, which made all of their best to support me. 3 Summary.....................................................………………………………………………... 5 1. Introduction...........................................................................................................……... 7 Prion diseases: early hypotheses...………...………………..........…......…......……….. 7 The basics of the prion concept………………………………………………….……... 8 Putative prion dissemination pathways………………………………………….……... 10 Earthworms: a putative factor of the dissemination of TSE infectivity in soil?.………. 11 Objectives of the study…………………………………………………………………. 16 2. Materials and Methods.............................…......................................................……….. 17 2.1 Sampling and general experimental design..................................................………. 17 2.2 Fluorescence in situ Hybridization (FISH)………..……………………….………. 18 2.2.1 FISH with soil, intestine, and casts samples…………………………….……... 18 Isolation of cells from environmental samples…………………………….………. -
NCTC) Bacterial Strain Equivalents to American Type Culture Collection (ATCC) Bacterial Strains
This list shows National Collection of Type Cultures (NCTC) bacterial strain equivalents to American Type Culture Collection (ATCC) bacterial strains. NCTC Number CurrentName ATCC Number NCTC 7212 Acetobacter pasteurianus ATCC 23761 NCTC 10138 Acholeplasma axanthum ATCC 25176 NCTC 10171 Acholeplasma equifetale ATCC 29724 NCTC 10128 Acholeplasma granularum ATCC 19168 NCTC 10172 Acholeplasma hippikon ATCC 29725 NCTC 10116 Acholeplasma laidlawii ATCC 23206 NCTC 10134 Acholeplasma modicum ATCC 29102 NCTC 10188 Acholeplasma morum ATCC 33211 NCTC 10150 Acholeplasma oculi ATCC 27350 NCTC 10198 Acholeplasma parvum ATCC 29892 NCTC 8582 Achromobacter denitrificans ATCC 15173 NCTC 10309 Achromobacter metalcaligenes ATCC 17910 NCTC 10807 Achromobacter xylosoxidans subsp. xylosoxidans ATCC 27061 NCTC 10808 Achromobacter xylosoxidans subsp. xylosoxidans ATCC 17062 NCTC 10809 Achromobacter xylosoxidans subsp. xylosoxidans ATCC 27063 NCTC 12156 Acinetobacter baumannii ATCC 19606 NCTC 10303 Acinetobacter baumannii ATCC 17904 NCTC 7844 Acinetobacter calcoaceticus ATCC 15308 NCTC 12983 Acinetobacter calcoaceticus ATCC 23055 NCTC 8102 acinetobacter dna group 13 ATCC 17903 NCTC 10304 Acinetobacter genospecies 13 ATCC 17905 NCTC 10306 Acinetobacter haemolyticus ATCC 17907 NCTC 10305 Acinetobacter haemolyticus subsp haemolyticus ATCC 17906 NCTC 10308 Acinetobacter johnsonii ATCC 17909 NCTC 10307 Acinetobacter junii ATCC 17908 NCTC 5866 Acinetobacter lwoffii ATCC 15309 NCTC 12870 Actinobacillus delphinicola ATCC 700179 NCTC 8529 Actinobacillus equuli ATCC 19392 -
Culture Media Edition for Industrial Microbiology LABORATORIOS CONDA S.A
2nd Edition for Industrial Microbiology Culture Media LABORATORIOS CONDA S.A. Edited by: Laboratorios Conda S.A. © 2013. Conda S.A. All rights reserved. Printed in Spain C/ La Forja, 9 28850 - Torrejón de Ardoz, Madrid - SPAIN Tel. +34 91 761 02 00 Fax +34 91 656 82 28 C/ Berlín, 63 08029 Barcelona - SPAIN Tel. +34 93 363 72 64 / 65 Fax. +34 93 363 72 61 [email protected] [email protected] www.condalab.com Index 6 Meat & Fish Industry 20 Beer Industry 10 Water & Beverages 21 Waste Water 11 Dairy Products 23 Cosmetic Industry 15 Bakery 24 Pharmaceutical Industry 17 Processed Foods 25 Microbiology Dehydrated Culture Media Guide 19 Wines iv Media for Industrial Microbiology CULTURE MEDIA FOR INDUSTRIAL MICROBIOLOGY | 2ND EDITION Media for Industrial Microbiology 5 Culture Media for Industrial Microbiology Laboratorios CONDA, one of the world USP and AOAC standards. Strict quality leaders in the design and manufacturing of control procedures are adopted prior to, high quality culture media, currently offers during and after the manufacturing process more than 400 different products, among to ensure quality products and batch-to-batch which you will find chromogenic media, ISO- consistency. We also exert tight control over formulated media and custom-made media selection and treatment of all raw materials for many different industrial applications. and components (peptones, carbohydrates, minerals, chemicals, agar and other additives) From hygiene control, through food used in the manufacturing process. Physical- and beverage poisoning prevention, to chemical characteristics are tested, and microbiologial examination of cosmetic and media also undergo additional microbiological pharmaceutical products, CONDA supplies a tests that guarantee growth, differentiation, wide variety of different media for each field biochemical performance, recovery of small so that customers can find the most suitable inocula, selectivity, etc. -
PDF, Effect of Differences in Salt Concentration on the Quality Of
IOP Conference Series: Earth and Environmental Science PAPER • OPEN ACCESS Effect of Differences in Salt Concentration on the Quality of Rebon Shrimp Paste (Acetes Sp) in Tegal District To cite this article: S Mulyani et al 2021 IOP Conf. Ser.: Earth Environ. Sci. 755 012051 View the article online for updates and enhancements. This content was downloaded from IP address 170.106.33.19 on 26/09/2021 at 20:52 ACHOST 2020 IOP Publishing IOP Conf. Series: Earth and Environmental Science 755 (2021) 012051 doi:10.1088/1755-1315/755/1/012051 Effect of Differences in Salt Concentration on the Quality of Rebon Shrimp Paste (Acetes Sp) in Tegal District S Mulyani 1*, P M Vestiyati 1, Kusnandar 1, H K Alamsyah 1, and S W Simanjuntak 1 1Faculty of Fisheries and Marine Science, Pancasakti University of Tegal, Indonesia *[email protected] Abstract. Rebon Shrimp Paste (RSP) in Indonesia uses different percentages of salt addition, ranging from 2 to 20% or not at all. This study aims to determine the influence of different salt concentration (5%, 10%, 15% and without salt) on the quality of RSP organoleptic, microbiological and chemical. This research was conducted in Munjung Agung, Tegal and Cirebon Fisheries Product Quality Testing and Application Laboratory. The results showed that the addition of different salt concentration (5%, 10%,15% and without salt) affected the quality of organoleptics, microbiology, and chemistry. Organoleptic quality with salt concentration of 5% and 10% favored panelists with an average value of 6.8 (not yet meeting Indonesian National Standards). The highest water content value is found in RSP that are not added salt (40,19%-43,22%) and lowest at 15% salt concentration (31,12%-34,82%) in accordance with the SNI. -
The Risk to Human Health from Free-Living Amoebae Interaction with Legionella in Drinking and Recycled Water Systems
THE RISK TO HUMAN HEALTH FROM FREE-LIVING AMOEBAE INTERACTION WITH LEGIONELLA IN DRINKING AND RECYCLED WATER SYSTEMS Dissertation submitted by JACQUELINE MARIE THOMAS BACHELOR OF SCIENCE (HONOURS) AND BACHELOR OF ARTS, UNSW In partial fulfillment of the requirements for the award of DOCTOR OF PHILOSOPHY in ENVIRONMENTAL ENGINEERING SCHOOL OF CIVIL AND ENVIRONMENTAL ENGINEERING FACULTY OF ENGINEERING MAY 2012 SUPERVISORS Professor Nicholas Ashbolt Office of Research and Development United States Environmental Protection Agency Cincinnati, Ohio USA and School of Civil and Environmental Engineering Faculty of Engineering The University of New South Wales Sydney, Australia Professor Richard Stuetz School of Civil and Environmental Engineering Faculty of Engineering The University of New South Wales Sydney, Australia Doctor Torsten Thomas School of Biotechnology and Biomolecular Sciences Faculty of Science The University of New South Wales Sydney, Australia ORIGINALITY STATEMENT '1 hereby declare that this submission is my own work and to the best of my knowledge it contains no materials previously published or written by another person, or substantial proportions of material which have been accepted for the award of any other degree or diploma at UNSW or any other educational institution, except where due acknowledgement is made in the thesis. Any contribution made to the research by others, with whom 1 have worked at UNSW or elsewhere, is explicitly acknowledged in the thesis. I also declare that the intellectual content of this thesis is the product of my own work, except to the extent that assistance from others in the project's design and conception or in style, presentation and linguistic expression is acknowledged.' Signed ~ ............................ -
Evaluation of Duopath Legionella Kit for the Rapid Identification Of
Biocontrol Science, 2007, Vol.12, No.4, 155-158 Note Evaluation of DuopathLegionella Kit for the Rapid Identification of Legionella Strains Isolated from Water Samples HIROAKI INOUE1•–, TOMOKO TAKAMA1, YUKIKO AGAWA1, JUNKO ONODERA1, TOMOKI ISHIMA1, KUNIO AGATA1, KEIKO SAITOH2, AND KATSUNORI HURUHATA3 1 Tsukuba Research Laboratories , Aquas Corporation, 4-4 Midorigahara, Tsukuba, lbaraki 300-2646, Research and Investigation Department, Building2 Manegement Education Center, 1-4-28, Mita, Minato, Tokyo 108-0073, School of Environmental Health, Azabu University, 3 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan Received 20 August, 2007/Accepted 18 October, 2007 Duopath Legionella (Merck KGaA, Darmstadt, Germany) is a rapid and simple immunochromatographic assay kit for the identification of Legionella species. We evaluated the precision of the kit in identifying 100 strains of Legionella and 35 strains of non-Legionella bacteria isolated from cooling tower and bath water samples. Consequently, of all the Legionella strains tested, 99 strains were judged to be Legionella, and only one strain (Legionella busanensis) was judged to be non-Legionella. All of the 35 non-Legionella strains were judged to be non-Legionella. We therefore conclude that Duopath Legionella is a useful method for the rapid identification of Legionella. Key words: Identification/Immunochromatography/Legionella. Legionella species are gram-negative bacteria are required to obtain the final results of the test, be- ubiquitously found in various aquatic environments. If cause the growth of Legionella on the selective agar humans were to inhale aerosolized water from a plates is very slow. Therefore, the development of a source contaminated with Legionella, such as cooling rapid detection and identification procedure for tower or bath water, they could contract a severe Legionella by the culture method is desired. -
Food Microbiology
Food Microbiology Food Water Dairy Beverage Online Ordering Available Food, Water, Dairy, & Beverage Microbiology Table of Contents 1 Environmental Monitoring Contact Plates 3 Petri Plates 3 Culture Media for Air Sampling 4 Environmental Sampling Boot Swabs 6 Environmental Testing Swabs 8 Surface Sanitizers 8 Hand Sanitation 9 Sample Preparation - Dilution Vials 10 Compact Dry™ 12 HardyCHROM™ Chromogenic Culture Media 15 Prepared Media 24 Agar Plates for Membrane Filtration 26 CRITERION™ Dehydrated Culture Media 28 Pathogen Detection Environmental With Monitoring Contact Plates Baird Parker Agar Friction Lid For the selective isolation and enumeration of coagulase-positive staphylococci (Staphylococcus aureus) on environmental surfaces. HardyCHROM™ ECC 15x60mm contact plate, A chromogenic medium for the detection, 10/pk ................................................................................ 89407-364 differentiation, and enumeration of Escherichia coli and other coliforms from environmental surfaces (E. coli D/E Neutralizing Agar turns blue, coliforms turn red). For the enumeration of environmental organisms. 15x60mm plate contact plate, The media is able to neutralize most antiseptics 10/pk ................................................................................ 89407-354 and disinfectants that may inhibit the growth of environmental organisms. Malt Extract 15x60mm contact plate, Malt Extract is recommended for the cultivation and 10/pk ................................................................................89407-482 -
WO 2016/188962 Al 1 December 2016 (01.12.2016) P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2016/188962 Al 1 December 2016 (01.12.2016) P O P C T (51) International Patent Classification: (74) Agents: GOODFELLOW, Hugh Robin et al; Carpmaels C12Q 1/68 (2006.01) & Ransford LLP, One Southampton Row, London WC1B 5HA (GB). (21) International Application Number: PCT/EP2016/061599 (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (22) Date: International Filing AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, 23 May 20 16 (23.05.2016) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (25) Filing Language: English DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (26) Publication Language: English KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, (30) Priority Data: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, 1508860.2 22 May 2015 (22.05.2015) GB PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, (71) Applicant: NATIONAL UNIVERSITY OF IRELAND, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. GALWAY [IE/IE]; University Road, Galway (IE). (84) Designated States (unless otherwise indicated, for every (72) Inventors: REDDINGTON, Kate Mary; Deerpack East, kind of regional protection available): ARIPO (BW, GH, Newport Road, Westport, Co. -
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 Research Journal of Pharmaceutical, Biological and Chemical Sciences Florula of Larval and Imaginal Phases of the Volfartova Fly (Wohlfarthia magnifica) In the Conditions of the Steppe Zone of The Pavlodar Region. A A Bitkeyeva1* and L T Bulekbayeva2. 1Senior teacher, Master of Ecology, Pavlodar State University named after S. Toraygyrov, The Republic of Kazakhstan. 2Associate professor, Candidate of Biological Sciences, Pavlodar State Pedagogical Institute, Republic of Kazakhstan. ABSTRACT Groups of bacteria were found during research in a steppe zone of the Pavlodar region, belonging to 3 families: Baccilaceae, Micrococcaceae, Enterobacteriacea. 13 species of pathogenic and opportunistic bacteria are obtained and identified, which cause diseases. Reception of agents from flies of Wohlfartia magnifica family in region farms forces to pay attention to quite real possibility and contagion of various infections. It creates the menacing epidemiological and epizootiology situation on the adjacent to farms of populated places, as flies with excrements can infect forages and migrate on considerable distances. Keywords: bacteria, diseases, infections, larvaes, microorganisms, flies, sheep, pathogenic microorganisms, carriers. *Corresponding author July– August 2015 RJPBCS 6(4) Page No. 2069 ISSN: 0975-8585 INTRODUCTION Flies are known as carriers of causative agents of dangerous infectious and invasive diseases. Therefore, in the populated places and on the pastures, studying of microbal and helminthosis impurity of flies represents scientific and practical interest. Epidemiological value of flies was opened by E.N. Pavlovskiy and V.P. Derbeneva-Ukhova, they participate in distribution about 70 pathogenic microflora, and including agents of a tularemia, anthrax, diphtheria, cholera, plague, a crab hand, etc. [2; 8; 12]. -
Aquascreen® Legionella Species Qpcr Detection Kit
AquaScreen® Legionella species qPCR Detection Kit INSTRUCTIONS FOR USE FOR USE IN RESEARCH AND QUALITY CONTROL Symbols Lot No. Cat. No. Expiry date Storage temperature Number of reactions Manufacturer INDICATION The AquaScreen® Legionella species qPCR Detection kit is specifically designed for the quantitative detection of several Legionella species in water samples prepared with the AquaScreen® FastExt- ract kit. Its design complies with the requirements of AFNOR T90-471 and ISO/TS 12869:2012. Legionella are ubiquitous bacteria in surface water and moist soil, where they parasitize protozoa. The optimal growth temperature lies between +15 and +45 °C, whereas these gram-negative bacteria are dormant below 20 °C and do not survive above 60 °C. Importantly, Legionella are well-known as opportunistic intracellular human pathogens causing Legionnaires’ disease and Pontiac fever. The transmission occurs through inhalation of contami- nated aerosols generated by an infected source (e.g. human-made water systems like shower- heads, sink faucets, heaters, cooling towers, and many more). In order to efficiently prevent Legionella outbreaks, water safety control measures need syste- matic application but also reliable validation by fast Legionella testing. TEST PRINCIPLE The AquaScreen® Legionella species Kit uses qPCR for quantitative detection of legionella in wa- ter samples. In contrast to more time-consuming culture-based methods, AquaScreen® assays need less than six hours including sample preparation and qPCR to reliably detect Legionella. Moreover, the AquaScreen® qPCR assay has proven excellent performance in terms of specificity and sensitivity: other bacterial genera remain undetected whereas linear quantification is obtai- ned up to 1 x 106 particles per sample, therefore requiring no material dilution. -
Clinical Microbiology 12Th Edition
Volume 1 Manual of Clinical Microbiology 12th Edition Downloaded from www.asmscience.org by IP: 94.66.220.5 MCM12_FM.indd 1 On: Thu, 18 Apr 2019 08:17:55 2/12/19 6:48 PM Volume 1 Manual of Clinical Microbiology 12th Edition EDITORS-IN-CHIEF Karen C. Carroll Michael A. Pfaller Division of Medical Microbiology, Departments of Pathology and Epidemiology Department of Pathology, The Johns Hopkins (Emeritus), University of Iowa, University School of Medicine, Iowa City, and JMI Laboratories, Baltimore, Maryland North Liberty, Iowa VOLUME EDITORS Marie Louise Landry Robin Patel Laboratory Medicine and Internal Medicine, Infectious Diseases Research Laboratory, Yale University, New Haven, Connecticut Mayo Clinic, Rochester, Minnesota Alexander J. McAdam Sandra S. Richter Department of Laboratory Medicine, Boston Department of Laboratory Medicine, Children’s Hospital, Boston, Massachusetts Cleveland Clinic, Cleveland, Ohio David W. Warnock Atlanta, Georgia Washington, DC Downloaded from www.asmscience.org by IP: 94.66.220.5 MCM12_FM.indd 2 On: Thu, 18 Apr 2019 08:17:55 2/12/19 6:48 PM Volume 1 Manual of Clinical Microbiology 12th Edition EDITORS-IN-CHIEF Karen C. Carroll Michael A. Pfaller Division of Medical Microbiology, Departments of Pathology and Epidemiology Department of Pathology, The Johns Hopkins (Emeritus), University of Iowa, University School of Medicine, Iowa City, and JMI Laboratories, Baltimore, Maryland North Liberty, Iowa VOLUME EDITORS Marie Louise Landry Robin Patel Laboratory Medicine and Internal Medicine, Infectious Diseases Research Laboratory, Yale University, New Haven, Connecticut Mayo Clinic, Rochester, Minnesota Alexander J. McAdam Sandra S. Richter Department of Laboratory Medicine, Boston Department of Laboratory Medicine, Children’s Hospital, Boston, Massachusetts Cleveland Clinic, Cleveland, Ohio David W. -
BD Industry Catalog
PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80