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UC Merced Department Submission No. 1 University: UC Merced Department: Molecular Cell Biology College: Biological sciences Category: Undergraduate Name: Jesselynn LaBelle E‐Mail Address: [email protected] Faculty Mentor: Stephanie Woo Were Human Subjects Used? No Regarding Data Collected, is it Ongoing or Concluded?: Ongoing Presentation Type: Poster Presentation Project Title: TAEL 2.0: An improved optogenetic gene expression system for zebrafish Inducible gene expression systems are valuable tools for studying biological processes. We previously developed an optogenetic gene expression system called TAEL that is optimized for use in zebrafish. When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL‐responsive C120 promoter. By using light as the inducing agent, the TAEL/C120 system overcomes limitations of traditional inducible expression systems by enabling fine spatial and temporal regulation of gene expression. Here, we describe ongoing efforts to improve the TAEL/C120 system. We made modifications to both the TAEL transcriptional activator and the C120 regulatory element, collectively referred to as “TAEL 2.0.” We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system. With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or in the developing endoderm. We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system. We believe this improved optogenetic expression system will be a useful resource for the zebrafish community. Submission No. 2 University: California State University, Fresno Department: Public Health College: College of Health and Human Services Category: Faculty Name: Tanisha Garcia E‐Mail Address: [email protected] Faculty Mentor: Kara Zografos Were Human Subjects Used? No Regarding Data Collected, is it Ongoing or Concluded?: Concluded Presentation Type: Poster Presentation Project Title: Has The Affordable Care Act Influenced Cardiology Disease Rates In The San Joaquin Valley? This study evaluated the effects of the Affordable Care Act (ACA) healthcare reform on Cardiovascular Disease (CVD) within the San Joaquin Valley (SJV). The sample consisted of Patient Discharge Data collected from the Office of Statewide Planning and Development (OSHPD) from the years 2010 ‐2017. The report provides a summary of CVD in the San Joaquin Valley and the burden held on residents despite the increased number of insured under the ACA. Key findings include: Did the ACA impact the distribution of insurance coverage for circulatory hospitalizations in older adults living in the SJV? To what extent does the drop in utilization of county indigent programs and self‐pay account for the increase in Medi‐Cal use? Hypothesis incites that access to healthcare in the SJV increased due to the ACA. As a result, CVD patients from the age of 40‐64 declined. With the data, we show how many individuals appeared in 2013 with county indigent or self‐pay and changed in 2015 to Medi‐Cal. Additionally, regression statistics provided the likelihood of people changing insurance from county indigent or self‐pay to Medi‐ Cal. The evaluation of this data demonstrates the positive impact the ACA has on the SJV for those suffering from CVD. Any changes made to the ACA could be detrimental to the existing state of CVD in the SJV. Thus, causing more economic burden to the state of California. Submission No. 3 University: California State University, Fresno Department: Chemistry College: College of Science and Mathematics Category: Graduate Name: Mike Jian E‐Mail Address: [email protected] Faculty Mentor: Mike Jian Were Human Subjects Used? No Regarding Data Collected, is it Ongoing or Concluded?: Concluded Presentation Type: Oral Presentation Project Title: Crystal structure of a cross‐species reactive nanobody in complex with human serum albumin Nanobodies are single‐domain antibodies derived from the unique heavy‐chain antibodies found in camelid species. Nanobodies are attractive therapeutics as they are easy to produce, are highly stable, and can bind targets inaccessible to traditional antibody formats. However, one disadvantage associated with nanobodies is rapid renal clearance resulting in a short serum half‐life. One innovative solution to increasing the serum half‐life of nanobodies is to make use of bispecific nanobodies. In this format, a therapeutic nanobody is fused to a second nanobody specific for the highly abundant plasma protein, serum albumin. Such bispecific nanobody constructs targeting serum albumins have been shown to increase the half‐life of therapeutic nanobodies. The use of an albumin‐specific nanobody that cross reacts with albumin found in commonly used research animals is particularly desirable as the bispecific nanobody could be used in pre‐clinical animal trials. Previously through immunization of a llama with human serum albumin, we have generated a panel nanobodies which cross‐react with the serum albumins of humans, macaques, mouse, and rat. To determine the basis of the cross‐species albumin reactivity of the nanobodies, we used X‐ray crystallography and obtained the structure of the nanobody M79 in complex with human serum albumin. The structure revealed that of the three nanobody binding loops, only two of the loops (CDR2 and CDR3) interacted with human serum albumin. The M79 nanobody bound to the apex of subdomain IIB on human serum albumin, specifically forming polar contacts with residues D308, D314, N318, and E321. Comparison of the serum albumin sequences from human, macaques, mouse, and rat show that the residues in serum albumin that bound M79 are highly conserved amongst the species explaining the nanobodies’ cross‐reactivity. These results give insights on the cross‐species specificity of M79 towards serum albumin, and can bolster the use of M79 in animal model experiments. Submission No. 4 University: University of California, Merced Department: Economics and Business Management College: Please see below Category: Undergraduate Name: Sang Hoang E‐Mail Address: [email protected] Faculty Mentor: Justin Hicks Were Human Subjects Used? No Regarding Data Collected, is it Ongoing or Concluded?: Ongoing Presentation Type: Poster Presentation Project Title: Stock Price and Investor Information Availability Information and investment decisions are directly related according to all fundamental models of corporate finance and economics. As such, I propose a direct measurement of capturing investor information with the use of Google Trends ‐ Search Volume Index (SVI). Using the SVI of companies in the S&P 500 and their monthly stock prices; and monthly closing stock prices extracted from the University of Pennsylvania’s Wharton Research Data Services database (WRDS) I ran a time‐series regression from 2008 to 2018 and find that there is a significant relationship between SVI’s web searches and the price of a stock 2 to 4 months from the time the search index makes a major change. The relationships between the SVI and the stock’s monthly price is negative and statistically significant at the 2 month lag. In my findings, A 3 standard deviation increase in web searches leads to a .48 dollar decrease in stock price 2 months out, controlling all attributes of the firm. The implications of this research findings confirm that Google Trends’ Search Volume Index can predict stock prices. Submission No. 5 University: University of California, Merced Department: Political Science College: College of Social Sciences Category: Undergraduate Name: Alondra Calderon E‐Mail Address: [email protected] Faculty Mentor: Elaine Denny Were Human Subjects Used? No Regarding Data Collected, is it Ongoing or Concluded?: Ongoing Presentation Type: Poster Presentation Project Title: Stress Amongst College Students In the University of California, Merced it was reported in the 2019 year alone, 52.3% of students were struggling with handing financial stress and 42.1% were struggling with overall stress. In addition, evidence suggested that students tend to have trouble with adjusting and fully being able to finish college because of overall stress put upon them. The purpose of this research is to understand the importance of the stress in students in order to find methods of coping and adjusting resources. An analysis done through this research showed that college students under more stressful circumstances tend to be less academically successful then those with on‐campus resource knowledge and use. Researchers job is to find the right mechanism to improve not only resources to deal with stress, but also how to adequately provide those resources to students. Submission No. 6 University: UC Merced Department: School of Natural science College: Math Category: Undergraduate Name: Ricky Yan E‐Mail Address: [email protected] Faculty Mentor: Chen Yangquan Were Human Subjects Used? No Regarding Data Collected, is it Ongoing or Concluded?: Ongoing Presentation Type: Poster Presentation Project Title: Unmanned Ground Vehicle for Water Leak Detection The Unmanned Ground Vehicle (UGV) project is the future of the next generation’s agricultural gadgets. Instead of the past’s archaic methods of manually searching for leaks with acoustic
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