insects Article Development of Single Nucleotide Polymorphism (SNP) Markers for Analysis of Population Structure and Invasion Pathway in the Coconut Leaf Beetle Brontispa longissima (Gestro) Using Restriction Site-Associated DNA (RAD) Genotyping in Southern China Zhiming Chen 1,2,3, Guihua Wang 1,2 , Min Li 4, Zhengqiang Peng 5, Habib Ali 1,2,6, Lina Xu 1,2, Geoff M. Gurr 1,2,7,* and Youming Hou 1,2,* 1 State Key Laboratory of Ecological Pest Control of Fujian-Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
[email protected] (Z.C.);
[email protected] (G.W.);
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[email protected] (L.X.) 2 Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 Rongcheng Customs District of China, Fuzhou 350015, China 4 Technology Center of Fuzhou Customs District, Fuzhou 350000, China;
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[email protected] 6 Department of Entomology, University of Agriculture Faisalabd, Sub Campus Depalpur, Okara 56300, Pakistan 7 Graham Centre, Charles Sturt University, Orange NSW 2800, Australia * Correspondence:
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[email protected] (Y.H.) Received: 13 February 2020; Accepted: 1 April 2020; Published: 7 April 2020 Abstract: Todetermine population genomic structure through high-throughput sequencing techniques has revolutionized research on non-model organisms. The coconut leaf beetle, Brontispa longissima (Gestro), is a widely distributed pest in Southern China. Here, we used restriction site-associated DNA (RAD) genotyping to assess the invasion pathway by detecting and estimating the degree of genetic differentiation among 51 B.