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WO 2009/046384 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date (10) International Publication Number 9 April 2009 (09.04.2009) PCT WO 2009/046384 Al (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 15/82 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT,AU, AZ, BA, BB, BG, BH, BR, BW, BY,BZ, CA, (21) International Application Number: CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, PCT/US2008/078860 EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, (22) International Filing Date: 3 October 2008 (03.10.2008) LR, LS, LT, LU, LY,MA, MD, ME, MG, MK, MN, MW, (25) Filing Language: English MX, MY,MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY,TJ, (26) Publication Language: English TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW (30) Priority Data: 60/978,059 5 October 2007 (05.10.2007) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant (for all designated States except US): DOW GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, AGROSCIENCES LLC [US/US]; 9330 Zionsville Road, ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), Indianapolis, IN 46268 (US). European (AT,BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, (72) Inventors; and FR, GB, GR, HR, HU, IE, IS, IT, LT,LU, LV,MC, MT, NL, (75) Inventors/Applicants (for US only): SAMUEL, Jayaku- NO, PL, PT, RO, SE, SI, SK, TR), OAPI (BF, BJ, CF, CG, mar, Pon [US/US]; 3336 Kilkenny Circle, Carmel, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). IN 46032 (US). BURROUGHS, Frank [US/US]; 929 Queensbury Drive, Noblesville, IN 46062 (US). Published: ZETTLER, Mark, W. [US/US]; 11142 Saint Charles — with international search report Place, Carmel, IN 46033 (US). DIXIT, Suraj, K. [IN/US]; — before the expiration of the time limit for amending the 3242 Braeside Drive, Bloomington, IN 47408 (US). claims and to be republished in the event of receipt of amendments (74) Agents: CATAXINOS,Edgar, R. et al; TRASKBRITT, — with sequence listing part of description published sep a 230 South 500 East, Suite 300, Po. Box 2550, Salt Lake rately in electronic form and available upon request from City, UT 841 10-2550 (US). the International Bureau (54) Title: METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS (57) Abstract: Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS PRIORITY CLAIM This application claims the benefit of the filing date of United States Provisional Patent Application Serial Number 60/978,059, filed October 5, 2007, for METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS. BACKGROUND Nanoparticles have unique properties that have been exploited for use in the delivery of DNA to cells. Among all nanoparticles investigated gold (Au) nanoparticles tend to be excellent candidates for delivery because of their low cytotoxicity and ease of functionalization with various ligands of biological significance. The commonly used synthesis of Au nanoparticles yields negatively charged (e.g., citrate coating) surface. Plasmid DNA, which may be sufficiently flexible to partially uncoil its bases, can be exposed to gold nanoparticles ("GNPs"). Under these partially uncoiled conditions, the negative charge on the DNA backbone may be sufficiently distant so that attractive van der Waals forces between the bases and the gold nanoparticle are sufficient to cause plasmid DNA to be attached to the surface of the gold particle. hi addition to metal nanoparticles, semi-conductor nanoparticles (e.g., quantum dots) ("QD") within the size range of 3-5 nm have also been used as carriers to deliver molecules into cells. DNA and proteins can be linked to the ligand attached to the QD surface (see, e.g., Patolsky, F.,et al, J. Am. Chem. Soc. 125, 13918 (2003)). Carboxylic acid or amine coated QDs can be cross linked to molecules containing a thiol group see, e.g., Dubertret B, .et al., Science 298, 1759 (2002); Akerman, M. E., W. C. W. Chan, P. Laakkonen, S. N. Bhatia, E. Ruoslahti, Proc. Natl. Acad. Sci. U.S.A. 99, 12617 (2002); Mitchell, G. P., C. A. Mirkin, R. L. Letsinger, J. Am. Chem. Soc. 121, 8122 (1999)) or an N-hydroxysuccinimyl (NHS)ester group by using standard bioconjugation protocols (see, e.g., Pinaud, F. , D. King, H.-P. Moore, S. Weiss, J. Am. Chem. Soc. 126, 6 115 (2004); Brachez, M.., M. Moronne, P. Gin, S. Weiss, A. P. Alivisatos, Science 281, 2013 (1998)). An alternative way is conjugation of streptavidin coated QDs to biotinylated proteins, oligos or antibodies (see, e.g., Dahan M. et al., Science 302, 442 (2003); Pinaud, F., D. King, H.-P. Moore, S. Weiss, J. Am. Chem. Soc. 126, 6 115 (2004); Dahan M. et al., Science 302, 442 (2003); Wu . X. Y., et al, Nature Biotechnol. 21, 4 1 (2003); Jaiswal, J. K., H. Mattoussi, J. M. Mauro, S. M. Simon, Nature Biotechnol. 21, 47 (2003); and Mansson, A., et al., Biochem. Biophys. Res. Commun. 314, 529 (2004). Nanoparticles have been used to deliver plasmid DNA to a variety of animal cells. It has been found that when DNA coated nanoparticles are incubated with cells not having a cell wall, the cells take up the nanoparticles and begin expressing any genes encoded on the DNA. Where nanoparticle delivery to cells normally having a cell wall is desired, the cells wall is stripped before the addition of the particles to protoplasts of plant (see, Torney, F. et al., Nature Nanotechnol. 2, (2007). hi plant cells, the cell wall stands as a barrier for the delivery of exogenously applied molecules. Many invasive methods, like gene gun (biolistics), microinjection, electroporation, and Agrobacterium, have been employed to achieve gene and small molecule delivery into these walled plant cells, but delivery of proteins have only been achieved by microinjection. Delivery of small molecules and proteins in the presence of a cell wall of a plant cell remains unexplored and would be advantageous in order to develop enabling technologies to be deployed in intact plant cell/ tissue or organ for in vitro and in vivo manipulations The present invention relates to methods using nanoparticles to non-invasively deliver molecular substances into cells having a cell wall. DISCLOSURE OF INVENTION The following embodiments are described in conjunction with systems, tools and methods which are meant to be exemplary and illustrative, and not limiting in scope. According to the invention, there are provided methods of introducing a molecule of interest into a plant cell that includes a cell wall, the methods comprising: placing the plant cell having a cell wall in contact with a nanoparticle and a molecule of interest, and allowing uptake of the nanoparticle and the molecule of interest into the cell. Further provided are methods of introducing a molecule of interest into a plant cell having a cell wall, the methods comprising: placing the plant cell having a cell wall in contact with a nanoparticle and a molecule capable of treating the disease and allowing uptake of the nanoparticle and the molecule capable of treating the disease into the cell. In addition to the exemplary aspects and embodiments described above, further aspects and embodiments will become apparent in view of the following descriptions. BRIEF DESCRIPTION OF DRAWINGS FIG. 1 depicts photographs of single cells of BY2 viewed using a differential Interference Contrast microscope attached to a confocal imaging system (Panel A). Panel B shows a light microscopic view of a single cell from a BY2 variant that is stained with I2KI to highlight the plastid (Amyloplast). FIG. 2, Pane A depicts photoautotrophic cells of tobacco (NTl) maintained in minimal medium and 5% carbon dioxide as seen in a light microscope, where prominent chloroplasts are visible. FIG. 2, Panel B, shows similar NTl cells viewed under a fluorescent microscope with active chloroplasts autofluorescing in red. FIG. 3 shows BY2 suspension aggregates treated with SAMSA fluorescein alone and with SAMSA fluorescein coated GNPs. FIG. 3, Panel A, shows a DIC image of cells treated with SAMSA fluorescein alone while FIG. 3, Panel B, shows the fluorescent image of the same cells. FIG. 3, Panel C, shows a DIC image of cells treated with SAMSA fluorescein coated GNPs while FIG. 3, Panel D, shows the fluorescent image of the SAMSA fluorescein coated GNPs-treated cells. Positions of Nucleus (Nu) and Cell Wall (CW) are as indicated. FIG. 4 shows SAMSA fluorescein coated GNP-treated single cells under high magnification. Panel B shows the presence of large number of GNPs in the nucleolus. Panel A shows a bright-field view of the same nucleolus shown in Panel B under a different plane of focus. FIG. 5 shows photoautotrophic cells treated with SAMSA fluorescein coated GNP. Panel A shows hyaline cells in 3-4 cell clusters with large chloroplasts lining the inner side of the cell wall. Panel B shows accumulation of nanoparticles in the chloroplast. Panels C and D show higher power magnification of a single chloroplast using a fluorescent microscope. Nanoparticles are visible in the membrane lamellations of the chloroplast and interspersed among the red autofluorescing chlorophyll pigments.
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