Immunoperoxidase Technique in Histopathology: Applications, Methods, and Controls
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CCR7 Antibody (Y59) NB110-55680
Product Datasheet CCR7 Antibody (Y59) NB110-55680 Unit Size: 0.1 ml Store at -20C. Avoid freeze-thaw cycles. Publications: 9 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/NB110-55680 Updated 5/28/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/NB110-55680 Page 1 of 6 v.20.1 Updated 5/28/2019 NB110-55680 CCR7 Antibody (Y59) Product Information Unit Size 0.1 ml Concentration This product is unpurified. The exact concentration of antibody is not quantifiable. Storage Store at -20C. Avoid freeze-thaw cycles. Clonality Monoclonal Clone Y59 Preservative 0.01% Sodium Azide Isotype IgG Purity Tissue culture supernatant Buffer 49% PBS, 0.05% BSA and 50% Glycerol Target Molecular Weight 45 kDa Product Description Host Rabbit Gene ID 1236 Gene Symbol CCR7 Species Human, Mouse, Rat, Monkey Reactivity Notes May cross reacts with Rhesus monkey. Specificity/Sensitivity The antibody does not cross-react with other G-protein coupled receptor 1 family members. Immunogen A synthetic peptide corresponding to residues in N-terminal extracellular domain of human CKR7 was used as immunogen. Notes Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Product Application Details Applications Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunoprecipitation, Flow Cytometry (Negative) Recommended Dilutions Western Blot 1:1000-10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:250, Immunoprecipitation 1:10, Immunohistochemistry-Paraffin 1:250, Immunohistochemistry-Frozen 1:250, Flow Cytometry (Negative) Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. -
Memoranaurns by the Participants in Signes Par Les Partici- I the Meeting
Memoranda are state- Les Memorandums ments concerning the exposent les conclu- /e , conclusions or recom- sions et recomman- M e mmooranrantedaa mendations of certain dations de certaines / a t w / /WHO scientific meet- /reunions scientifiques ings; they are signed de /'OMS; ils sont Memoranaurns by the participants in signes par les partici- I the meeting. pants a ces reunions. Bulletin ofthe World Health Organization, 62 (2): 217-227 (1984) © World Health Organization 1984 Immunodiagnosis simplified: Memorandum from a WHO Meeting* Technologies suitable for the development ofsimplified immunodiagnostic tests were reviewed by a Working Group of the WHO Advisory Committee on Medical Research in Geneva in June 1983. They included agglutination tests and use ofartificialparticles coated with immunoglobulins, direct visual detection of antigen-antibody reactions, enzyme- immunoassays, and immunofluorescence and fluoroimmunoassays. The use of mono- clonal antibodies,in immunodiagnosis and of DNA /RNA probes to identify viruses was also discussed in detail. The needfor applicability of these tests at three levels, i.e., field conditions (or primary health care level), local laboratories, and central laboratories, was discussed and their use at thefield level was emphasized. Classical serological techniques have been used for All these tests can be carried out in laboratories that a long time for diagnostic purposes, e.g., for con- are equipped with basic instruments as well as special- firmation of clinical diagnoses, epidemiological ized apparatus (e.g., gamma counters for RIA, ultra- studies, testing of blood donors, etc. Some of these violet microscopes for IMF, etc.), which are usually techniques have been standardized to a high degree of available only in the larger, central laboratories. -
Cytokine Immunocytochemistry Fax (65) 860-1590 Fax (49) 40 531 58 92 Fax (81) 3 541-381-55 E-Mail: [email protected]
PharMingen International United States Canada PharMingen Europe Japan PharMingen Canada Tel 619-812-8800 Asia Pacific Becton Dickinson GmbH Nippon Becton Dickinson Toll-Free 1-888-259-0187 Orders 1-800-848-6227 BD Singapore HQ PharMingen Europe Company Ltd. Tel 905-542-8028 Tech Service 1-800-825-5832 Tel (65) 860-1478 Tel (49) 40 532 84 48 0 Tel (81) 3 541-382-51 Fax 905-542-9391 Fax 619-812-8888 Cytokine Immunocytochemistry Fax (65) 860-1590 Fax (49) 40 531 58 92 Fax (81) 3 541-381-55 e-mail: [email protected] http://www.pharmingen.com Africa Germany Latin/South America Spain Becton Dickinson Worldwide Inc. Becton Dickinson GmbH BDIS (USA) Becton Dickinson S.A. Reagents and Techniques Tel (254) 2 449 608 HQ PharMingen Europe Tel (408) 954-2157 Tel (34) 91 848 8100 Fax (254) 2 449 619 Tel (49) 40 532 84 48 0 Fax (408) 526-1804 Fax (34) 91 848 8105 Fax (49) 40 531 58 92 Orders for Microscopic Analysis of Cytokine-Producing Cells Australia Malaysia Tel (34) 91 848 8182 Becton Dickinson Pty Ltd Greece Becton Dickinson Sdn Bhd Fax (34) 91 848 8104 Tel (612) 9978-6800 Becton Dickinson Hellas S.A. Tel (03) 7571323 Fax (612) 9978-6850 Tel (30) 1 9407741 Fax (03) 7571153 Sweden Fax (30) 1 9407740 Becton Dickinson AB Austria Mexico Tel (46) 8 775 51 00 Becton Dickinson GmbH Hong Kong Becton Dickinson de Mexico Fax (46) 8 645 08 08 HQ PharMingen Europe Becton Dickinson Asia Ltd Tel (52-5) 237-12-98 Tel (49) 40 532 84 48 0 Tel (852) 2572-8668 Fax (52-5) 237-12-93 Switzerland Fax (49) 40 531 58 92 Fax (852) 2520-1837 Becton Dickinson GmbH Middle East HQ PharMingen Europe Belgium Hungary Becton Dickinson Tel (41) 06 1-385 4422 Becton Dickinson Benelux N.V. -
Ultrastructural Localization of Glial Fibrillary Acidic Protein in Mouse Cerebellum by Immunoperoxidase Labeling
Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Schachner, M. 1977. “Ultrastructural Localization of Glial Fibrillary Acidic Protein in Mouse Cerebellum by Immunoperoxidase Labeling.” The Journal of Cell Biology 75 (1) (October 1): 67–73. doi:10.1083/jcb.75.1.67. Published Version 10.1083/jcb.75.1.67 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:32705572 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA ULTRASTRUCTURAL LOCALIZATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN MOUSE CEREBELLUM BY IMMUNOPEROXIDASE LABELING M. SCHACHNER, E. T. HEDLEY-WHYTE, D. W. HSU, G. SCHOONMAKER, and A. BIGNAMI From the Department of Neuroiogy-Neuropathology, Harvard Medical School and the Departments of Neuroscience and Pathology (Neuropathology) and the Mental Retardation Research Center, Children's Hospital Medical Center, Boston, Massachusetts 02115. Dr. Bignami's present address is Spinal Cord Injury Research, West Roxbury V. A. Hospital, West Roxbury, Massachusetts 02132. ABSTRACT Glial fibrillary acidic protein was localized at the electron microscope level in the cerebellum of adult mice by indirect immunoperoxidase histology. In confirma- tion of previous studies at the light microscope level, the antigen was detectable in astrocytes and their processes, but not in neurons or their processes, or in oligodendroglia. -
Diagnostic Potential of the Haemagglutination
Bulgarian Journal of Veterinary Medicine (2007), 10 , N o 3, 169 −178 DIAGNOSTIC POTENTIAL OF THE HAEMAGGLUTINATION INHIBITION TEST, THE IMMUNODIFFUSION TEST AND ELISA FOR DETECTION OF ANTIBODIES IN CHICKENS, INTRAVENOUSLY INFECTED WITH A/DUCK/BULGARIA/05 H6N2 AVIAN INFLUENZA VIRUS ISOLATE I. S. ZARKOV Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria Summary Zarkov, I., 2007. Diagnostic potential of the haemagglutination inhibition test, the immuno- diffusion test and ELISA for detection of antibodies in chickens, intravenously infected with A/duck/Bulgaria/05 H6N2 avian influenza virus isolate. Bulg. J. Vet. Med. , 10 , No 3, 169 −178. After experimental infection of chickens with an avian influenza viral isolate A/duck/Bulgaria/05 H6N2, the potential of the haemagglutination inhibition test (HIT), the immunodiffusion test (IDT) and enzyme-linked immunosorbent assay (ELISA) for antibody detection was evaluated. The results evidenced that in birds, haemagglutinins, precipitins and IgG antibodies, detectable with ELISA, were formed. The percentage of chickens with subtype-specific antibodies was the highest by the 21 st day of infection (100 %), followed by the 14 th (66.7 %) and the 28 th (55.6 %) days, and was the lowest by the 7 th day (44.4 %). Serum titres ranged between 1:4 and 1:256 with predomination of 1:8 and 1:16 titres (29.2 % each). The mean arithmetic titre for the experiment was 1:38.2. The highest percentage of chickens with precipitins was observed by the 14th day (55.6 %) followed by the 7 th , 21 st and the 28 th days with 33.3 % each. -
AG 39: Immunofluorescence Assays (PDF)
ibidi Application Guide Immunofluorescence Assays The Principle of Immunofluorescence Immunofluorescence Applied: Assays . 2 Experimental Examples . 13 Rat Hippocampal Neuron and Astrocyte Staining 14 Immunofluorescence Staining: Visualization of Endothelial Cell Junctions . 13 A Typical Workflow . 3 Immunostaining of Rat Dorsal Root Ganglionic Experiment Planning and Sample Preparation . 4 Cells and Schwann Cells . 13 Sample Fixation . 4 Adherens Junctions and Actin Cytoskeleton of Cell Permeabilization . 5 HUVECs Under Flow . 14 Blocking . 5 Mitochondria Staining of MDCK cells . 14 Primary Antibody Incubation . 5 Focal Adhesions of Differentiated Mouse Fibroblasts on an Elastic Surface . 15 Secondary Antibody Incubation . 6 Counterstain and Mounting . 7 Microscopy . 7 Troubleshooting . 8 Selected Publications Immunofluorescence C. Xu, et al. NPTX2 promotes colorectal cancer growth and liver With the ibidi Chambers . 9 metastasis by the activation of the canonical Wnt/beta-catenin pathway via FZD6. Cell Death & Disease, 2019, 10.1038/s41419- Comparison of Immunocytochemistry Protocols . 10 019-1467-7 Chambered Coverslips . 11 read abstract Kobayashi, T., et al. Principles of early human development and Channel Slides . 11 germ cell program from conserved model systems. Nature, Chamber Slides . 12 2017, 10.1038/nature22812 read abstract H. Tada et al. Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells. PloS one, 2016, 10.1371/journal.pone.0152794 read abstract N. J. Foy, M. Akhrymuk, A. V. Shustov, E. I. Frolova and I. Frolov. Hypervariable Domain of Nonstructural Protein nsP3 of Venezuelan Equine Encephalitis Virus Determines Cell-Specific Mode of Virus Replication. Journal of Virology, 2013, 10.1128/ jvi.00720-13 read abstract .com The Principle of Immunofluorescence Assays Immunofluorescence (IF) is a powerful approach for getting insight into cellular structures and processes using microscopy . -
Serological Methods in the Identification and Characterization of Viruses
CHAPTER 4 Serological Methods in the Identification and Characterization of Viruses M. H. V. Van Regenmortel Laboratoire de Virologie Institut de Biologie Mo!eculaire et Cellulaire 67000 Strasbourg, France 1. INTRODUCTION The purpose of this chapter is to present an integrated view of the various serological techniques that have been used in virology. The accent will be placed on the principles that govern each type of test and on the general applicability of the different serological techniques in all fields of virus research. In recent years, advances in serological tech niques have sometimes been applied in only one area of virology, although they could have been equally useful to workers studying other groups of viruses. No doubt this stems from the host-oriented approach that has guided the compartmentation of virology into separate fields of specialization. When it comes to serological properties, however, the similarities between animal, insect, bacterial, and plant viruses are paramount. The same immunochemical principles govern the in vitro serological reactions of all viral antigens, and much of general interest can be learned from the findings obtained with each particular group of viruses. An attempt will be made here to emphasize the general validity of specific experimental procedures. A number of recent reviews restricted to the serology of particular groups of viruses are available 183 H. Fraenkel-Conrat et al. (eds.), Comprehensive Virology © Plenum Press, New York 1981 184 Chapter 4 (Cowan, 1973; Schmidt and Lennette, 1973; Ball, 1974; Kurstak and Morisset, 1974; Burns and Allison, 1975; Mazzone and Tignor, 1976; Mayr et al., 1977; Tyrrell, 1978; Van Regenmortel, 1978; Cooper, 1979). -
116588NCJRS.Pdf
If you have issues viewing or accessing this file contact us at NCJRS.gov. " "'- u.s. Department of Justice Federal Bureau of Investigation PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON FORENSIC IMMUNOLOGY FBI ACADEMY QUANTICO, VIRGINIA JUNE 23-26, 1986 Proceedings of the International Symposium on Forensic Immunology co 00 L() \0 u, r-l O§ r-l Ql C. If) 05~ Ql 0- 0 ~ o~ Ql E C/) Ql >. c:0 If) C/) ~ * ....,a: () a: z Ql '*02 Ql iii :5 ...... ....,::> a Ql {ij "...:0- Ql c: !!lc: °E ::>;: B 00 {ij c: 0 ~I::> z~ "e CD ~~ :5 .... ~o- S 1::C: Lt .~ Host Laboratory Division Federal Bureau of Investigation June 23-26, 1986 Forensic Science Research and Training Center FBI Academy Quantico, Virginia NOTICE This publication was prepared by the U. S. Government. Neither the U. S. Government nor the U. S. Department of Justice nor any of their employees makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness or usefulness of any information, apparatus, product or process disclosed, or represents that in use would not infringe privately owned rights. Reference herein to any specific commercial product, process or service by trade name, mark, manufacturer or otherwise does not necessarily constitute or imply its endorsement, recommendation or favoring by the U. S. Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the U. S. Government or any agency thereof. Published by: The Laboratory Division Roger T. Castonguay Assistant Director in Charge Federal Bureau of Investigation U. -
Improved Cervical Smear Assessment Using Antibodies Against Proteins That Regulate DNA Replication
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 14932–14937, December 1998 Medical Sciences Improved cervical smear assessment using antibodies against proteins that regulate DNA replication GARETH H. WILLIAMS*†‡,PIOTR ROMANOWSKI*§,LESLEY MORRIS*†,MARK MADINE*§,ANTHONY D. MILLS*§, KAI STOEBER*§,JACKIE MARR*§,RONALD A. LASKEY*§, AND NICHOLAS COLEMAN† *Wellcome TrustyCancer Research Campaign Institute, and Departments of §Zoology and †Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom Communicated by Paul Nurse, Imperial Cancer Research Fund, London, United Kingdom, September 24, 1998 (received for review June 11, 1998) ABSTRACT Carcinoma of the cervix is one of the most of cancer and has proven highly effective in reducing cervical common malignancies. Papanicolaou (Pap) smear tests have cancer mortality and morbidity rates. The Pap test samples reduced mortality by up to 70%. Nevertheless their interpre- approximately 500,000–600,000 superficial surface cells from tation is notoriously difficult with high false-negative rates the epithelium of the cervix (exfoliative cytology). Smear and frequently fatal consequences. We have addressed this preparations are made from these samples and screened for problem by using affinity-purified antibodies against human the presence of precursor malignant (dysplastic) cells by using proteins that regulate DNA replication, namely Cdc6 and morphological criteria. If detected early, cervical cancer is Mcm5. These antibodies were applied to sections and smears easily treated. of normal and diseased uterine cervix by using immunoper- However, despite the introduction of mass screening pro- oxidase or immunofluorescence to detect abnormal precursor grams, the best of which have reduced mortality rates by 70%, malignant cells. Antibodies against Cdc6 and Mcm5 stain incidence of cervical cancer in the United States has been abnormal cells in cervical smears and sections with remark- increasing by about 3% a year since 1986 in spite of an ably high specificity and sensitivity. -
Laboratory Analysis of the Immune Response 859
Chapter 20 | Laboratory Analysis of the Immune Response 859 Chapter 20 Laboratory Analysis of the Immune Response Figure 20.1 Lab-on-a-chip technology allows immunological assays to be miniaturized so tests can be done rapidly with minimum quantities of expensive reagents. The chips contain tiny flow tubes to allow movement of fluids by capillary action, reactions sites with embedded reagents, and data output through electronic sensors. (credit: modification of work by Maggie Bartlett, NHGRI) Chapter Outline 20.1 Polyclonal and Monoclonal Antibody Production 20.2 Detecting Antigen-Antibody Complexes 20.3 Agglutination Assays 20.4 EIAs and ELISAs 20.5 Fluorescent Antibody Techniques Introduction Many laboratory tests are designed to confirm a presumptive diagnosis by detecting antibodies specific to a suspected pathogen. Unfortunately, many such tests are time-consuming and expensive. That is now changing, however, with the development of new, miniaturized technologies that are fast and inexpensive. For example, researchers at Columbia University are developing a “lab-on-a-chip” technology that will test a single drop of blood for 15 different infectious diseases, including HIV and syphilis, in a matter of minutes.[1] The blood is pulled through tiny capillaries into reaction chambers where the patient’s antibodies mix with reagents. A chip reader that attaches to a cell phone analyzes the results and sends them to the patient’s healthcare provider. Currently the device is being field tested in Rwanda to check pregnant women for chronic diseases. Researchers estimate that the chip readers will sell for about $100 and individual chips for $1.[2] 1. -
Design and Methods Antibodies
Design and methods Antibodies Primary antibodies: Details of the antibodies used as primary reagents for immunostaining germinal center T cells are given in Table 1. The anti-SAP antibody used in this study was a commercial polyclonal reagent. A monoclonal antibody of the same specificity (anti-SH2D1A, Cell Signaling Technology, Inc, Boston, MA, Cat. No. 2805) was tested on tonsil sections and a limited number of diffuse large B cell lymphoma cases, and showed the same reactivity pattern as the polyclonal antibody. The anti-PD-1 antibody was a monoclonal reagent produced following immunization of a BALB/c mouse with the natural killer cell line YT (DSMZ, Braunschweig, Germany). Splenic cells were fused with a mouse myeloma cell line as previously described (1). A hybridoma secreting an antibody designated NAT, that gave selective immunolabeling of T cells in germinal centers, was chosen for cloning and further study. The antibody is of IgG1 subtype and was used as undiluted tissue culture supernatant. Western blotting against protein lysates from the YT cell line showed that it reacted with a molecule of approximately 47 kDa molecular weight (Figure 5 in the main text). This is similar to the molecular weight reported for the PD-1 molecule, and the immunohistologic and flow cytometric reactivity of antibody NAT was also similar to that observed with a known anti-PD-1 antibody (data not shown). To confirm that the monoclonal antibody NAT recognized human PD-1 protein, a pCMV6- XL5-PD-1 vector (Homo sapiens programmed cell death 1 (NM-005018.1), Origene, USA) expressing human PD-1 was transfected into HEK293T cells (Human embryonal kidney cell line, ATCC, Manassas,Va,USA). -
Virology Techniques
Chapter 5 - Lesson 4 Virology Techniques Introduction Virology is a field within microbiology that encom- passes the study of viruses and the diseases they cause. In the laboratory, viruses have served as useful tools to better understand cellular mechanisms. The purpose of this lesson is to provide a general overview of laboratory techniques used in the identification and study of viruses. A Brief History In the late 19th century the independent work of Dimitri Ivanofsky and Martinus Beijerinck marked the begin- This electron micrograph depicts an influenza virus ning of the field of virology. They showed that the agent particle or virion. CDC. responsible for causing a serious disease in tobacco plants, tobacco mosaic virus, was able to pass through filters known to retain bacteria and the filtrate was able to cause disease in new plants. In 1898, Friedrich Loef- fler and Paul Frosch applied the filtration criteria to a disease in cattle known as foot and mouth disease. The filtration criteria remained the standard method used to classify an agent as a virus for nearly 40 years until chemical and physical studies revealed the structural basis of viruses. These attributes have become the ba- sis of many techniques used in the field today. Background All organisms are affected by viruses because viruses are capable of infecting and causing disease in all liv- ing species. Viruses affect plants, humans, and ani- mals as well as bacteria. A virus that infects bacteria is known as a bacteriophage and is considered the Bacteriophage. CDC. Chapter 5 - Human Health: Real Life Example (Influenza) 1 most abundant biological entity on the planet.