Scottish Society of Cytomics SSC 2014
“Transla onal Cytometry from Bench to Bedside”
1st Annual Mee ng on 25th September 2014
With an exci ng line up of Speakers, Workshops & Awards!
Ins tute of Medical Sciences Level 7 Conference Room University of Aberdeen Foresterhill, Aberdeen
Local Organisers: Linda Duncan, Raif Yuecel, David Wilson & Janice Forsyth
Free Registra on and Lunch kindly provided by generous sponsors: Please send your registra on to [email protected] “Transla onal Cytometry from Bench to Bedside”
KEY SPEAKERS TOPICS Paul Smith (University of Cardiff) Regenera ve Medicine Derek Davies (CRUK London) Cancer Research Ian Dimmick (University of Newcastle) Drug Discovery Cosimo De Bari (University of Aberdeen) Clinical Cytometry John Campbell (NSL Edinburgh) Immunology Adriano Rossi (MRC Edinburgh) Data Analysis Tools Elizabeth Ballou (University of Aberdeen) Mul colour Cytometry David Wilson (NHS Grampian) Fungal Research
SPECIAL LECTURE: Paul Smith Past President of ISAC & Emeritus Professor of Cancer Biology University of Cardiff Cytomics Of The Tumour Microenvironment: Therapeu c Targe ng or “Loca on, Loca on, Loca on!”
SPECIAL WORKSHOP: Ian Dimmick Manager, Flow Cytometry Core Facility, Newcastle upon Tyne University Prac cal session on 8-10 colour Compensa on
1st Annual meeting of SSC 2014 Contents
4 SSC Programme for 25th of September
7 Speaker Biographies
14 Registra on and Poster Award Info
16 Sponsors and Exhibi on Plan
20 Direc ons and Map of IMS
21 Aberdeen City Map
23 Post-SSC Mee ng Workshop on 26th of September “Flow Cytometry Data Analysis Workshop”
1st Annual meeting of SSC 2014 3 PROGRAMME
Thursday 25th September
08:15 Coffee Recep on Registra on (Free) and Poster Displaying (IMS level 7 CR)
09:00 Welcome by Raif Yuecel, SSC, IFCC, University of Aberdeen 09:10 Welcome by Peter McCaffery, IMS Director, University of Aberdeen 09:20 Welcome by Alistair Brown, Integrated Centre, University of Aberdeen
Speakers
09:30 Derek Davies, CRUK London Sharing the love: Networking amongst cytometrists
09:45 Elizabeth Ballou, University of Aberdeen FACS for Fungi: Revealing popula on heterogeneity and dynamics in fungal pathogens via flow cytometry
10:00 Ian Dimmick, Newcastle upon Tyne University A unique approach to compensa on, and its verifica on
10:20 David Wilson, NHS Grampian Quality in Clinical Cytometry - do we con nue to make the same mistakes?
10:40 COFFEE & Poster Session
11:00 Derek Davies, CRUK London Drug effects in mul ple rounds of cell division monitored by flow cytometry
11:20 Cosimo De Bari, University of Aberdeen Cell therapies for joint repair
11:40 John Campbell, Na onal Science Laboratory Edinburgh Transla ng Research Flow Assays for Characteriza on of Cellular Therapeu cs
12:00 Adriano Rossi, MRC Edinburgh Flow cytometric techniques for isola ng and analysing leucocytes
1st Annual meeting of SSC 2014 4 12:20 Special Lecture: Paul Smith, University of Cardiff Cytomics Of The Tumour Microenvironment: Therapeu c Targe ng, or Loca on, Loca on, Loca on!
13:00 LUNCH & Exhibi on (IMS Atrium)
AFTERNOON Parallel Sessions
14:00 - 16:00 Prac cal session on 8-10 colour Compensa on by Ian Dimmick , IMS, IFCC lab 2.54 Registra on is necessary as the number is limited to max 20!
14:00 - 16:00 New technologies by industrial applica on specialists at IMS level 7 CR: Please see the next page.
16:00 - 17:00 SSC Commi ee mee ng (open mee ng, IMS level 7)
17: 00 Come together over Wine and Cheese & Poster Award Winner Announcement (IMS level 7, everyone welcome)
1st Annual meeting of SSC 2014 5 A ernoon parallel session from 14:00 – 16:00 at IMS level 7 CR New technologies by industrial applica on specialists (10min presenta ons)
Roy Bongaerts, Sony Biotechnology Europe New mul colour single cell technology: Spectral cell analysis on the Sony SP6800
Laura Schneider, Cell Signaling Technology, NEB Ltd Considera ons to study cell signaling by flow cytometry
Stephen Rackstraw, BD Biosciences Sor ng the Molecules that Count’ - Single Cell Sor ng for Next Genera on Sequencing’
Jackie Su er, Kalpana Singh, Miltenyi Biotech Ltd MACS Flow Cytometry Products: Innovate Solu ons for all Your Flow Needs
Lindsey Ward, iLab Solu ons LLC Addressing the Challenges of Opera ng a Core Facility
Leonor Heleno Wielgosz, Stratocore Facility Management bringing the focus back to Research
Jasmin Moss Life Technologies, Thermo Fisher A une® NxT: The next genera on in acous c focusing cytometry
Sco Cribbes, Nexcelcom Ltd. Adherent Imaging Cytometry Complemen ng Flow Cytometric Measurements to address the diverse needs of a research environment
Andrea Valle, De Novo So ware Cytometry Data Analysis using FCS Express
Norman Maidment, Beckman Coulter Ltd Going Dry: Alterna ve monoclonal an body op ons for Flow Cytometry
eBioscience (TBC)
1st Annual meeting of SSC 2014 6 Keynote Lecturer
Paul Smith Emeritus Professor of Cancer Biology Institute of Cancer & Genetics, Innovation and Engagement Representative, Cardiff University, UK
Paul has been active in academic research in the fields of DNA repair, drug development, cytometry, biochip and imaging for more than 30 years at the MRC Centre in Cambridge and at Cardiff University where he is now Emeritus Professor of Cancer Biology. His research expertise has focused on cancer - encompassing the cell cycle, cancer stem cells and resistance to anticancer drugs. His inventions include a range of anticancer drugs and molecular probes including the DRAQ dyes. He has served the International Society for Advancement of Cytometry, being the President from 2010-12 and successfully introducing the CYTO series of congresses with linked educational initiatives. Earlier, he was responsible for the ISAC’s 1st International Cytomics Meeting in Wales in 2003, aimed at introducing the concept of cytomics. His entrepreneurial activities include the co-founding of the technology companies Biostatus Ltd and Biosuspensions Ltd. He is the Science Director of the award- winning start-up Oncotherics Ltd – a new pharmaceutical company that has exploited cytometric techniques in the development of novel hypoxia-activated pro- drugs for the treatment of a wide range of cancers.
1st Annual meeting of SSC 2014 7 Speakers( ( Derek(Davies(
Manager'of'the'FACS'Laboratory,'London'Research'Institute'(LRI),'' Cancer'Research'UK,'London,'UK' ' ' Derek’s'first'post'after'graduating'was'in'a'regional' Cytopathology'Unit'where,'amongst'other'duties,'he' was' introduced' to' cytometry' ' F' using' a' microdensitometer' to' measure' DNA' content' in' cervical'cells.'This'led'to'his'first'exposure'of'a'flow' cytometer'–'the'FACS'Analyzer.' Realizing' the' potential' for' cytometry' in' cancer' diagnosis' and' research,' he' moved' to' Kings' College' Hospital' in' London' on' an' MRCFfunded' project' to' look' at' the' feasibility' of' using' a' flowFbased'preFscreen'in'cytological'specimens.' In'1990,'he'moved'to'the'Imperial'Cancer'Research'Fund'to'work'in'what'was'then'a' small' core' facility' providing' support' for' 40' research' Laboratories.' Derek' became' Head'of'that'unit'in'1996'and'remains'there'today.'Derek'currently'manages'a'team' of'7'staff'and'9'cytometers'(6'analysers'and'3'sorters).' Derek'is'Chair'of'flowcytometryUK,'he'sits'on'the'Council'of'the'Royal'Microscopical' Society'and'head'of'the'ISAC'Core'Managers'TaskForce.' He' is' very' active' in' teaching' cytometry' within' the' UK' and' coForganises' an' annual' meeting' on' behalf' of' the' RMS' as' well' as' speaking' at' several' other' national' and' international'courses.' Current'Research'Interest:' Development'of'instrumentation'and'methodologies'appropriate'to'the'needs'of'a' cancer'research'institute.' ' '
1st Annual meeting of SSC 2014 8 Elizabeth*Ballou**
Research( Fellow( in( the( Aberdeen( Fungal( Group( at( Institute( of( Medical( Sciences,( University(of(Aberdeen,(UK( ( Elizabeth(received(her(PhD(in(Genetics(and(Genomics( from( Duke( University,( USA,( in( the( lab( of( Dr.( Andrew( Alspaugh,( where( she( studied( the( role( of( conserved( RhoGGTPases( in( C.( neoformans( thermotolerance,( polarity,( and( ploidy.( As( a( member( of( Prof( Alistair( Brown's( lab( in( the( AFG,( Elizabeth( has( used( flow( cytometry( and( FACS( analysis( to( study( the( impact( of( host(carbon(source(on(C.(albicans(morphogenesis(and( pathogenicity.(( Current(Research(Interest:( Her( work( focuses( on( the( impact( of( the( human( host( environment( on( the( fungal( pathogens(Candida(albicans(and(Cryptococcus(neoformans.(
David&Wilson&&
Principal) Healthcare) Scientist,) Immunology) Laboratory,) Aberdeen) Royal) Infirmary,) UK) He) has) over) thirty) years) experience) in) clinical) flow)cytometry) working) both) in) Aberdeen) and) Glasgow.))David)is)a)founder)member)of)the)British) Society) for)Histocompatibility) and)Immunogenetics) and)is)a)Fellow)of)the)Institute)of)Biomedical)Science) and) a) member) of) their) Immunology) Scientific) Advisory) Panel.)) His) interests) in) addition) to) flow)cytometry) include) transplantation) and) autoimmune)diseases.)
1st Annual meeting of SSC 2014 9 Ian$Dimmick$
Manager,(Flow(Cytometry(Core(Facility,(Newcastle(upon(Tyne(University,(UK( ( (Ian( began( his( career( in( a( clinical( setting( and( from( 1972(to(1991(worked(in(various(research(posts(using( flow( cytometry( as( a( diagnostic( and( research( tool( primarily(for(HIV,(leukaemia,(lymphoma(and(a(broad( range(of(immunological(testing.( ( From(1991(to(2006(Ian(worked(as(a(Flow(Cytometry( Applications( and( Instrumentation( Specialist( for( various(commercial(companies.( Ian(took(up(his(current(post(in(2006(and(manages(the(flow(cytometry(core(facility(in( Newcastle( upon( Tyne( University.( In( his( current( role( Ian( organises( various( flow( cytometry( teaching( courses( and( conferences,( such( as( the( quarterly( ‘Colour( Compensation’( course( and( the( annual( ‘Practicalities( of( Flow( Cytometry/Cellular( Analysis’(meeting,(both(held(in(Newcastle(upon(Tyne.( Current(Research(Interests( Research( and( clinical( flow( cytometry( in( particular( leukaemia,( lymphoma( and( stem( cells.( ( (
1st Annual meeting of SSC 2014 10 Cosimo&De&Bari&
Professor'of'Translational'Medicine'&'Hon'Consultant'Rheumatologist' Institute'of'Medical'Sciences,'University'of'Aberdeen,'UK' ' Cosimo' is' a' clinically' active' rheumatologist' with' expertise' in' regenerative' medicine' for' musculoskeletal'applications.' Cosimo'graduated'in'Medicine'(maxima'cum'laude)' from' the' University' of' Bari' (Italy),' where' he' also' underwent' specialist' training' in' Rheumatology.' ' He' then'moved'to'Belgium,'where'he'obtained'his'PhD' from' the' University' of' Leuven' and' was' recipient' of' the' Rotary' Young' Investigator' Award' 2003' from' the' Belgian' Society' for' Rheumatology.' ' His' work' in' Belgium' contributed' to' the' development' of' an' autologous'cellular'product'for'articular'cartilage'repair,'which'recently'became'the' first' cellPbased' product' to' obtain' EU' Marketing' Authorisation' as' an' Advanced' Therapy'Medicinal'Product.'
In' 2003' Cosimo' moved' to' the' UK' in' the' Department' of' Rheumatology' at' King's' College'London.''In'May'2005'he'was'awarded'a'Clinician'Scientist'Fellowship'from' the' Medical' Research' Council' and' in' December' 2005' he' was' appointed' Clinical' Senior'Lecturer'&'Consultant'Rheumatologist.'
Since' September' 2007' Cosimo' is' Professor' of' Translational' Medicine' at' the' University' of' Aberdeen,' where' he' currently' leads' the' Musculoskeletal' Research' Programme.'
Cosimo'has'expertise'in'stem'cell'research'for'musculoskeletal'repair,'regenerative' medicine'and'tissue'engineering.''Current'research'interests'in'his'Group'include'(i)' the' development' of' stem' cellPbased' tissue' engineering' products' for' cartilage' and' bone'repair;'(ii)'the'study'of'the'stem'cell'niches'in'the'joint'in'health'and'diseases' such' as' osteoarthritis' and' rheumatoid' arthritis;' (iii)' the' investigation' of' the' developmental'ontogeny'of'mesenchymal'stem'cells.'
1st Annual meeting of SSC 2014 11 Adriano(G(Rossi(
Professor'of'Respiratory'and'Inflammation'' MRC'Centre'for'Inflammation'Research,'Queen's'Medical'Research'Institute' University'of'Edinburgh,'UK' ' Adriano' G.' Rossi' received' his' BSc' and' PhD' from' the' University'of'Glasgow,'Scotland'UK.'' He'carried'out'postdoctoral'research'at'Wake'Forest' University,' North' Carolina,' USA' and' at' the' National' Heart'&'Lung'Institute,'London,'UK.'' He' currently' holds' a' Chair' in' Respiratory' and' Inflammation' Pharmacology' at' the' University' of' Edinburgh,'with'a'research'focus'on'the'mechanisms' regulating'the'resolution'of'inflammation.'He'was'awarded'a'DSc'from'the'University' of' Edinburgh' and' is' a' Fellow' of' the' Society' of' Biology' and' a' Fellow' of' the' British' Pharmacological'Society.'' Research' Theme:' Immune' Modulation' and' Regulation' of' Inflammation,' Imaging' Inflammation.'We'aim'to'gain'a'better'understanding'of'the'mechanisms'controlling' inflammatory' processes' with' a' view' to' help' develop' novel' therapies' for' chronic' inflammatory' diseases.' ' For' this' we' aim' to' elucidate' the' mechanism' regulating' inflammatory'cell'behavior'and'apoptosis'and'manipulate'the'processes'controlling' the' resolution' of' inflammation' in' order' to' develop' new' therapeutic' strategies' to' remove'unwanted'and'dysregulated'inflammation.' '
1st Annual meeting of SSC 2014 12 John%Campbell%
Associate)Director)at)Research,)Development)&)Innovation,)SNBTS,)National)Science) Laboratory,)Hon)Reader)at)University)of)Edinburgh,)UK) ) John) is) Associate) Director) of) Research) and) Development) at) the) Scottish) National) Blood) Transfusion) service) (SNBTS)) in) Edinburgh.) He) completed)his)PhD)in)Pathology)at)Edinburgh)in)1995) on) the) immunopathogenesis) of) lymproliferative) disease,)and)has)worked)in)the)cellular)therapy)field) for)over)20)years.)) He) has) held) various) academic) positions) including) Lecturer)in)Tumour)Immunology)at)the)University)of)Glasgow.)He)has)also)worked) extensively) in) the) biotech) industry) as) R&D) project) leader,) Group) Manager) Clinical) Science) and) Cell) Analysis,) and) Director) of) Clinical) Immunology) at) Miltenyi) Biotec) GmbH.)He)returned)to)full)time)academic/healthcare)work)at)the)end)of)2012.)) John)is)currently)the)national)head)of)research)for)SNBTS)and)is)Hon.)Reader)at)the) University) of) Edinburgh.) SNBTS) has) a) substantial) cellular) therapy) research) programme,) with) over) 25) full) time) scientists) working) on) basic) cellular) function;) translation)of)laboratory)protocols)to)full)GMP)processes;)and)production)of)cellular) therapeutics)for)treatment)of)patients.)SNBTS)has)a)dedicated,)fully)MHRA)licensed,) GMP) cellular) therapy) production) centre) at) the) Scottish) Centre) for) Regenerative) medicine.)Cellular)therapeutics)currently)in)early)phase)trials)include)haematopoietic) stem) cells) for) liver) repair,) Corneal) Limbal) Stem) Cells) and) EBVYspecific) Cytotoxic) T) Lymphocytes)for)PTLD.)) John’s)own)research)programme)focuses)on)the)generation)of)novel)methodologies) to)characterise)and)improve)the)efficacy)and)safety)of)novel)cellular)therapeutics)in) vivo,)including)targeting)of)injected)cells)to)specific)anatomical)sites.) )
1st Annual meeting of SSC 2014 13 Registra on is FREE, but..
Full conference registra on and admission to all SSC sessions such as workshops and parallels, exhibi on, lunch, opening recep on and the closing ceremony are FREE.
However, we need the approx. number of a endees for catering purposes. Also, don’t forget to register for the DEMO session in the a ernoon as numbers for it are limited to 20! Please send your registra on and if you wish to a end the demo session to [email protected] by 10th of September 2014
SSC Poster Award
We would like to recognize the excellent research by scien sts who have applied cytometry (flow and/or imaging) in their studies by offering poster awards. Cash prizes and a cer ficate will be awarded to the winners: • 1st Poster prize £400 • 2nd Poster prize £200 • 3rd Poster prize £100
All scien sts who are students or postdoctoral researchers (who have received their doctorate within the last five years) are invited to submit their poster. The posters will be judged during the coffee break and the winners will be presented and recognized during the closing ceremony at 5pm at IMS level 7.
Deadline
Please submit your poster abstract by 15th of September 2014 to [email protected]
1st Annual meeting of SSC 2014 14 Registration+Form+ ! 1st!SSC!Meeting!on!25th!of!September!at!Institute!of!Medical!Sciences,!Level!7! Conference!Room,!University!of!Aberdeen,!Foresterhill,!Aberdeen! ! ! Free!Registration!and!Lunch!kindly!provided!by!generous!sponsors.! ! Please!send!your!registration!to!our!local!organizer!at!! [email protected]+ th by#10 !of#September#2014! Local!Organizers:!Linda!Duncan,!Raif!Yuecel,!David!Wilson!&!Janice!Forsyth! ! !
Name! ______!
Position:! ______!
Company:! ______!
ESMail:! ______!
Tel:! ______!
Address:! ______! ! + ! Dietary+Requirements:+ ______! Wi4Fi+Access:! !Yes!!!/!!!!No! ! ! Registration+Selections:! ! !!!Practical!Demo!Session! Yes!!!/!!!!No! !!!SSC!Member! Yes!!!/!!!!No! !!!Future!Member! Yes!!!/!!!!No! POSTER+Submission+ Yes!!!/!!!!No! !
1st Annual meeting of SSC 2014 15 1st SSC Mee ng Venue We are grateful to the Ins tute of Medical Sciences at the University of Aberdeen for their support and providing us an excellent loca on for our first SSC conference.
1st Annual meeting of SSC 2014 16 1st SSC Mee ng Sponsors We would like to acknowledge the following sponsors for their generous support and helping us to make this conference a big success:
1st Annual meeting of SSC 2014 17 Extra Dona ons We are especially grateful to the following contributors for their extra dona ons and support to SSC 2014.
Dona on of An bodies for Demo session
Dona on for Catering
Contribu on for the 2nd Poster Prize
Dona on of 3rd Poster Prize
1st Annual meeting of SSC 2014 18 EXHIBITION Plan in IMS Atrium
The IMS Atrium is open the en re day. Lunch will take place in the exhibi on area.
1. New England BioLabs
2. Beckman Coulter
3. Affymetrix – eBioscience 1 2 19 4. Sysmex-Partec 3 5. Stratocore 18 4 6. Sony Table LUNCH 7. Merck Millipore 17 5 8. iLab Solu ons 6 16 9. Life Technologies Table LUNCH 10. Caltag Medsystems
11. BioRad 7 12. Cambridge Bioscience 8 13. Enzo Life Sciences
Coffee Shop 9 14. Biotechne
15. BioLegend 10 16. Becton Dickinson 11 17. Nexelcom
18. DeNovo So ware 15 14 13 12 19. Miltenyi Biotec
1st Annual meeting of SSC 2014 19 Maps and Direc ons Ins tute of Medical Sciences · University of Aberdeen · Foresterhill · Aberdeen · AB25 2ZD
The IMS is based on the Foresterhill site: On arrival at the IMS car park from Ashgrove Road West, the IMS main recep on area is located at the right hand end (West end) of the building.
FORESTERHILL
! IMS recep on
1st Annual meeting of SSC 2014 20 Aberdeen Map and loca ons Please visit also follwing website for any accomoda on informa on: h p://www.visitaberdeen.com/your-visit/ Schuh Sky Superdrug Timpson Trespass Primark Quiz M&S Magnolia Menkind Miss Selfridge Monsoon Accessorize Monsoon Children New Look Next O2 Oasis Ortak Pepperberry Phase Eight Phones 4 U Radley River Island Sky Superdrug Swarovski The Body Shop The Card Factory The Perfume Shop Thorntons Topman Topshop Vision Express Vodaphone Wallis Warehouse Warren James Watch Workshop WH Smith Scan Me Aberdeen to download the the download to
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22 Friday 26 September 2014 Flow Cytometry Data Analysis Workshop Come and learn how to save time turning your flow data into results with FCS Express software. • Easy to use - familiar Microsoft Powerpoint like interface • Publication quality graphics • Create PowerPoints and PDFs • Batch Processing • Heatmaps, bar charts & scatter plots • Powerful and flexible statistics • Cell Cycle and Proliferation • Import Accuri CFlow files automatically • Import FACS DIVA experiments • Also New FCS Express 4 Image Cytometry • Much, much, more......
5 10
4 Iain Fraser Cytometry Centre 10 CD 3+ 24.04 The Ins tute of Medical Sciences
3 10 Level 7 Conference Room
CD13+33 PE-A RSVP to [email protected] 2 -10
2 2 3 4 5 9:00am – 12:00pm -105 10 10 10 10 10 CD3 APC-A 4 Please bring any Ques ons! 10 Hope to see you there
3 10 Presented by: Andrea Valle CD13+33 PE-A 2 -10 Email : [email protected]
2 2 3 4 5 -10 10 10 10 10 CD3 APC-A
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