MOLECULAR AND CELLULAR Volume 8 February 1988 No.2

Swiveling and Decatenation of Replicating Simian Virus 40 Genomes In Vivo. Robert M. Snapka, Mark A. Powelson, and John M. Strayer ...... 515-521 Characterization of N-myc Amplification Units in Human Neuroblastoma Cells. B. A. Zehnbauer, D. Small, G. M. Brodeur, R. Seeger, and B. Vogelstein ...... 522-530 Activating Mutations for Transformation by p53 Produce a Gene Product That Forms an hsc70-p53 Complex with an Altered Half-Life. C. A. Finlay, P. W. Hinds, T.-H. Tan, D. Eliyahu, M. Oren, and A. J. Levine ...... 531-539 Neoplastic Transformation Induced by an Activated Lymphocyte-Specific Tyrosine Kinase (pps6kCk). Jamey D. Marth, Jonathan A. Cooper, Connie S. King, Steven F. Ziegler, Donald A. Tinker, Robert W. Overell, Edwin G. Krebs, and Roger M. Perlmutter ...... 540-550 Yeast STE7, STEII, and STE12 Genes Are Required for Expression of Cell-Type- Specific Genes. Stanley Fields, Deborah T. Chaleff, and George F. Sprague, Jr...... 551-556 The Calcium Signal for BALB/MK Terminal Differentiation Coun- teracts Epidermal Growth Factor (EGF) Very Early in the EGF-Induced Proliferative Pathway. Pier Paolo Di Fiore, Joseph Falco, Ivan Borrello, Bernard Weissman, and Stuart A. Aaronson ...... 557-563 An Integral Membrane Protein Antigen Associated with the Membrane Attach- ment Sites of Actin Microfilaments Is Identified as an Integrin PI-Chain. Pamela A. Maher and S. J. Singer ...... 564-570 Structural Characterization of the Human Platelet-Derived Growth Factor A- Chain cDNA and Gene: Alternative Exon Usage Predicts Two Different Pre- cursor . Fredrik Rorsman, Margaret Bywater, Timothy J. Knott, James Scott, and Christer Betsholtz ...... 571-577 The Human c-fpslfes Gene Product Expressed Ectopically in Rat Fibroblasts Is Nontransforming and Has Restrained Protein-Tyrosine Kinase Activity. Peter A. Greer, Kelly Meckling-Hansen, and Tony Pawson ...... 578-587 Transformation of Mouse BALB/c 3T3 Cells with Human Basic Fibroblast Growth Factor cDNA. Reiko Sasada, Tsutomu Kurokawa, Makoto Iwane, and Koichi Igarashi ...... 588-594 Expansions and Contractions of the Genetic Map Relative to the Physical Map of Yeast Chromosome III. Lorraine S. Symington and Thomas D. Petes .... 595-604 Different cis-Acting DNA Elements Control Expression of the Human Apolipopro- tein AI Gene in Different Cell Types. Kedarnath N. Sastry, Udo Seedorf, and Sotirios K. Karathanasis ...... 605-614 DNA-Binding Properties of the Drosophila melanogaster zeste Gene Product. Alka Mansukhani, Anja Crickmore, Peter W. Sherwood, and Michael L. Goldberg ...... 615-623 Transcriptional Properties of BmX, a Moderately Repetitive Silkworm Gene That Is an RNA Polymerase III Template. Ellen T. Wilson, Donald Peter Condliffe, and Karen U. Sprague ...... 624-631 Elevated Expression in pp60csrc Alters a Selective Morphogenetic Property of Epi- thelial Cells In Vitro without a Mitogenic Effect. Stephen L. Warren, Laura M. Handel, and W. James Nelson ...... 632-646

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Mutational Analysis of Upstream Activation Sequence 2 of the CYCI Gene of Sac- charomyces cerevisiae: a HAP2-HAP3-Responsive Site. Susan L. Forsburg and Leonard Guarente ...... 647-654 The HAP3 Regulatory Locus of Saccharomyces cerevisiae Encodes Divergent Overlapping Transcripts. Steven Hahn, Jennifer Pinkham, Randy Wei, Rebecca Miller, and Leonard Guarente ...... 655-663 ATRI, a Saccharomyces cerevisiae Gene Encoding a Transmembrane Protein Re- quired for Aminotriazole Resistance. Susumu Kanazawa, Monica Driscoll, and Kevin Struhl ...... 664-673 No Glycolipid Anchors Are Added to Thy-i Glycoprotein in Thy-i-Negative Mu- tant Thymoma Cells of Four Different Complementation Classes. Andreas Conzelmann, Annalisa Spiazzi, Claude Bron, and Robert Hyman ...... 674-678 The Rat oa-Tropomyosin Gene Generates a Minimum of Six Different mRNAs Coding for Striated, Smooth, and Nonmuscle Isoforms by Alternative Splic- ing. David F. Wieczorek, Christopher W. J. Smith, and Bernardo Nadal- Ginard ...... 679-694 Identification of Negative and Positive Regulatory Elements Associated with a Class I Major Histocompatibility Complex Gene. Rachel Ehrlich, Jean E. Maguire, and Dinah S. Singer ...... 695-703 v-src Mutations Outside the Carboxyl-Coding Region Are Not Sufficient To Fully Activate Transformation by pp6O-src in NIH 3T3 Cells. Sita Reddy, Peter

Yaciuk, Thomas E. Kmiecik, Paul M. Coussens, and David Shalloway.... 704-712 A Fetal Globin Gene Mutation in Ay Nondeletion Hereditary Persistence of Fetal Hemoglobin Increases Promoter Strength in a Nonerythroid Cell. Mark W. Rixon and Richard E. Gelinas ...... 713-721 A Group of Type I Genes on Human Chromosome 17: Characterization and Expression. Marjorie Rosenberg, Amlan RayChaudhury, Thomas B. Shows, Michelle M. Le Beau, and Elaine Fuchs ...... 722-736 Insertion and Excision of Caenorhabditis elegans Transposable Element Tcl. David Eide and Philip Anderson ...... 737-746 Effects of Single Base Substitutions within the Acanthamoeba castellanii rRNA Promoter on Transcription and on Binding of Transcription Initiation Factor and RNA Polymerase I. Preecha Kownin, Erik Bateman, and Marvin R. Paule ...... 747-753 Structural Organization and Functional Analysis of Centromeric DNA in the Fis- sion Yeast Schizosaccharomyces pombe. Barbara Fishel, Hanspeter Amstutz, Mary Baum, John Carbon, and Louise Clarke ...... 754-763 Amplification and Expression of Heterologous Ornithine Decarboxylase in Chinese Hamster Cells. Tsu-Rong Chiang and Lisa McConlogue ...... 764-769 Cell Cycle Expression of RNA Duplex Unwindase Activity in Mammalian Cells. Richard W. Wagner and Kazuko Nishikura ...... 770-777 Expression of a Drosophila melanogaster Acetylcholine Receptor-Related Gene in the Central Nervous System. Samuel C. Wadsworth, Lawrence S. Rosenthal, K. Lynn Kammermeyer, Matthew B. Potter, and Donald J. Nelson ...... 778-785 H-ras Activation in Benign and Self-Regressing Tumors (Keratoacanthomas) in Both Humans and an Animal Model System. Javier Leon, Hideko Kamino, Jacob J. Steinberg, and Angel Pellicer ...... 786-793 Dictyostelium discoideum Myosin: Isolation and Characterization of cDNAs Encod- ing the Essential Light Chain. Rex L. Chisholm, Alice M. Rushforth, Richard S. Pollenz, Edward R. Kuczmarski, and Sherrie R. Tafuri...... 794-801 Continued on following page Continued from preceding page

Premature Translation Termination Mediates Triosephosphate Isomerase mRNA Degradation. Ira 0. Daar and Lynne E. Maquat ...... 802-813 Gel Electrophoretic Isolation of Splicing Complexes Containing Ul Small Nuclear Ribonucleoprotein Particles. Martin Zillmann, Maria L. Zapp, and Susan M. Berget .814-821 SPT3 Is Required for Normal Levels of a-Factor and ot-Factor Expression in Saccharomyces cerevisiae. Joel N. Hirschhorn and Fred Winston .822-827 A Novel hsp70-Like Protein (P70) Is Present in Mouse Spermatogenic Cells. Randy L. Allen, Deborah A. O'Brien, and E. M. Eddy .828-832 Temperature-Sensitive Transport of Glycoproteins to the Surface of a Variant Mouse Lymphoma Cell Line. Mukund Nori and Michael R. Stallcup 833-842 DNA Sequence, Structure, and Tyrosine Kinase Activity of the Drosophila melano- gaster Abelson Proto-Oncogene Homolog. Mark J. Henkemeyer, Randy L. Bennett, Frank B. Gertler, and F. Michael Hoffmann .843-853 Stable Variant-Specific Transcripts of the Variant Cell Surface Glycoprotein Gene 1.8 Expression Site in Trypanosoma brucei. Cathy Shea and Lex H. T. Van der Ploeg .854-859 The Length of the Downstream Exon and the Substitution of Specific Sequences Affect Pre-mRNA Splicing In Vitro. Paul J. Furdon and Ryszard Kole 860-866 Two Distinct Mechanisms of Transcriptional Control Operate on c-myc during Differentiation of HL60 Cells. Ulrich Siebenlist, Peter Bressler, and Kathleen Kelly .867-874 Nematode Repetitive DNA with ARS and Segregation Function in Saccharomyces cerevisiae. Kevin M. Felsenstein and Scott W. Emmons .875-883 Constitutive Expression of a c-myb cDNA Blocks Friend Murine Erythroleukemia Cell Differentiation. Michael F. Clarke, Jolanta F. Kukowska-Latallo, Eric Westin, Michael Smith, and Edward V. Prochownik .884-892 The Cell-Specific Elastase I Enhancer Comprises Two Domains. Fred Kruse, Cynthia T. Komro, Carolyn H. Michnoff, and Raymond J. MacDonald.... 893-902 Sequence-Specific Interactions between a Cellular DNA-Binding Protein and the Simian Virus 40 Origin of DNA Replication. Wolfgang Traut and Ellen Fanning .903-911 The SPS100 Gene of Saccharomyces cerevisiae Is Activated Late in the Sporulation Process and Contributes to Spore Wall Maturation. David T. S. Law and Jacqueline Segall .912-922 Chicken Homolog of the mos Proto-Oncogene. Martin Schmidt, Marianne K. Oskarsson, Joyce K. Dunn, Donald G. Blair, Stephen Hughes, Friedrich Propst, and George F. Vande Woude .923-929 The Coupling between Enhancer Activity and Hypomethylation of K Immunoglob- ulin Genes Is Developmentally Regulated. Dawn E. Kelley, Brian A. Pollok, Michael L. Atchison, and Robert P. Perry .930-937 Suppression of Chromosomal Mutations Affecting M1 Virus Replication in Saccha- romyces cerevisiae by a Variant of a Viral RNA Segment (L-A) That Encodes Coat Protein. Hiroshi Uemura and Reed B. Wickner .938-944 Comparison of the Structure and Cell Cycle Expression of mRNAs Encoded by Two Histone H3-H4 Loci in Saccharomyces cerevisiae. Sharon L. Cross and M. Mitchell Smith .945-954 Gene Conversion Associated with Site-Specific Recombination in Yeast Plasmid pSRl. Hiroaki Matsuzaki, Hiroyuki Araki, and Yasuji Oshima .955-962

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An Oligomer Complementary to c-myc'mRNA Inhibits Proliferation of HL-60 Promyelocytic Cells and Induces Differentiation. Jeffrey T. Holt, Robert L. Redner, and Arthur W. Nienhuis ...... 963-973 Introduction of New Genetic Material into Human Myeloid Leukemic Blast Stem Cells by Retroviral Infection. Larry J. Smith and Samuel Benchimol ..... 974-977 DNA Sequence Analysis of Spontaneous Mutations in the SUP4-o Gene of Saccha- romyces cerevisie. Craig N. Giroux, Joseph R. A. Mis, Mary K. Pierce, Susanne E. Kohalmi, and Bernard A. Kunz ...... 978-981 Capturing Nuclear Sequence-Specific DNA-Binding Proteins by Using Simian Virus 40-Derived Minichromosomes. Uri Nir, Eric Fodor, and William J. Rutter ...... 982-987 Localization of a Repressive Sequence Contributing to B-Cell Specificity in the Immunoglobulin Heavy-Chain Enhancer. Judah Weinberger, Parmjit S. Jat, and Phillip A. Sharp ...... 988-992 Generation of a Mutant Form of Protein Synthesis Initiation Factor eIF-2 Lacking the Site of Phosphorylation by eIF-2 Kinases. Vinay K. Pathak, Daniel Schindler, and John W. B. Hershey ...... 993-995 Localization of a Highly Divergent Mammalian Testicular a Tubulin That Is Not Detectable in Brain. Norman B. Hecht, Robert J. Distel, Pamela C. Yelick, Susan M. Tanhauser, Catherine E. Driscoll, Erwin Goldberg, and Kenneth S. K. Tung ...... 996-1000 Polymerase Ill Transcription Factor B Activity Is Reduced in Extracts of Growth- Restricted Cells. John Tower and Barbara Sollner-Webb ...... 1001-1005 Expression of the Rat Myosin Light-Chain 2 Gene in Transgenic Mice: Stage Specificity, Developmental Regulation, and Interrelation with the Endogenous Gene. Moshe Shani, Itzhak Dekel, and Olla Yoffe ...... 1006-1009 ERRATUM Multiple Positive and Negative 5' Regulatory Elements Control the Cell-Type- Specific Expression of the Embryonic Skeletal Myosin Heavy-Chain Gene. Patrice F. Bouvagnet, Emanuel E. Strehler, Glenn E. White, Marie- Antoinette Strehler-Page, Bernardo Nadal-Ginard, and Vijak Mahdavi .... 1010

Date of Issue: 2 February 1988