Analyte Quarterly Vol. 1 2021 Look closer at your assays, platforms, and results in your multiplex and singleplex biomarker detection assays

• SMC™ (Single Molecule Counting) Assays • ELISAs & RIAs • MILLIPLEX® Assays • Custom Assay Development - SMC™ Technology - MILLIPLEX® Assays

The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. IMMUNOASSAY PLATFORMS 2 |SigmaAldrich.com/immunoassays Look CloseratourAdvantages immunoassays foryour research needs. Whetheryou are usingourindustry-leading MILLIPLEX detection,weare andmultiplexanalyte todelivering committed in sensitivity thebestpossible research applications. Withrecent technologicaladvances ofproteinquantification pushing thelimits Researchers rely oncommercialimmunoassays tostudybiomarker expression inawidevariety of We Strive fortheIdeal Immunoassay Complete ImmunoassayComplete Testing Platforms • • • • User Experience • • • Quality • • • Published Performance drive your biomarker projectfromhypothesis topublication. reliable measurements, tohelp asimplifieduserexperience, andknowledgeablescientificpartners examining singleproteinswithourtried-and-true ELISAsandRIAs, you expect canconfidently analysis usingtheSingleMoleculeCounting(SMC™)ultrasensitive immunoassay platform,or multiplex panelstobroadlysurvey multipleanalytes, sub-picogram/mLbiomarker performing State-of-the-art Belysa™ immunoassay software forpowerful analysis andreporting forassaysKnowledgeable in-houseandfield-basedscientificsupport andinstruments Comprehensive protocolswithrecommendations foroptimalsamplepreparation pre-optimizedKit components foreasy useandminimalpreparation steps only (RUO) andnotforuseinclinicalormedicaldiagnosticpurposes Manufactured infacilitieswhichare are ISO9001:2015compliant,ourproducts forresearch use measurements Assays developed testedforaccurate, andquality reproducible, andbiologicallyrelevant protein Over fourdecades ofimmunoassay development andmanufacturingexpertise Customizable, premixed, andbulkkitoptionstofit your exact research needs generatedusingourimmunoassaysOver withdata 14,000publishedpapers selectionacrossresearchBroadest analyte areas andspecies Single/Multiplex Dynamic range Sample size Instrument Sensitivity Assay MILLIPLEX Accurately measure upto Luminex 48 proteinsinasample ~25 µL(may bediluted) MILLIPLEX Luminex Up topg/mL Multiplex ® ® 3-4 log : FLEXMAP3D orany Luminex assay ® ® 200™ Panels ® , ®

SMC™ Applicationswith SMCxPRO™ Instrument undetectable proteins undetectable Explore previously Up tofg/mL SMCxPRO™ Singleplex 5-100 µL 4-5 log SMC™ Any ELISAreader, orgamma Trusted for standards protein quantitation ELISAs andRIAs ELISAs Any ELISAorRIA counter (forRIA) Up topg/mL 50-100 µL Singleplex 2-3 log ®

IMMUNOASSAY PLATFORMS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

NEW KITS Cat. No. Cat.No. 40-50021 40-50023 40-50030 EZIL6-98K 03-0196-00 03-0183-00 03-0195-00 03-0197-00 03-0199-00 HFIB1-100K PRCYTA-40K EZHIL8-100K Coming Soon! Coming Soon! Coming Soon! Coming Soon! Coming Soon! EZRBDG-110K EZRBDM-120K EZMCP1-99KRM , and ELISA Kits, and ELISA ® SigmaAldrich.com/immunoassays | 3 SigmaAldrich.com/immunoassays Non-Human Primate Human Species Human Human Species Human Human Human Human Human — — — — — Human Human Human Human Human — analyte offerings and order panels: ® Next Generation Versatile Multiplexing Next Generation Versatile System: INTELLIFLEX RUO ® xMAP Coming Soon Panels ® Drive Fluid PLUS (RUO) Fluid PLUS Drive ® INTELLIFLEX Performance Verification Kit Verification INTELLIFLEX Performance INTELLIFLEX INTELLIFLEX Calibration Kit Sheath Fluid PLUS (RUO) Sheath Fluid PLUS (RUO) Sheath Concentrate PLUS ® ® ® ® ® For customers in the United States, Canada, Denmark, Finland, Italy, Norway, Portugal, Spain, Portugal, Norway, Canada, Denmark, Finland, Italy, customers in the United States, For SigmaAldrich.com/milliplex-order United Kingdom start search here: your customers EMDMillipore.com/milliplex in all other countries start here: search your For Design and purchase your own kit Design and purchase your Search our MILLIPLEX Fibrosis Panel 1 Fibrosis Panel Instruments and Reagents xMAP Cytokine/Chemokine/Growth Factor Panel A Panel Cytokine/Chemokine/Growth Factor SMC™ Kits Kit RBD IgM SMC™ SARS-CoV-2 Kit High Sensitivity Immunoassay Tau SMC™ Total Instruments and Reagents xMAP Description Description SMC™ Kits Kit Sensitivity High Immunoassay SMC™ Total-α-Synuclein Kit SMC™ UCHL1 High Sensitivity Immunoassay Kit High Sensitivity Immunoassay SMC™ IL-2 Kit S1 IgA SMC™ SARS-CoV-2 Kit High Sensitivity Immunoassay SMC™ NPTX2 MILLIPLEX xMAP MAGPIX xMAP xMAP ELISA Kits ELISA Anti-RBD IgG SARS-CoV-2 ELISA Anti-RBD IgM SARS-CoV-2 Sandwich ELISA IL-6 Conferma™ Sandwich ELISA IL-8 Conferma™ Sandwich ELISA MCP-1 Conferma™ For Research Use Only. Not For Use In Diagnostic Procedures. Not For Use Only. Research For For Research Use Only. Not For Use In Diagnostic Procedures. Not For Use Only. Research For Coming Soon NEW! Single Molecule Counting Molecule NEW! Single MILLIPLEX (SMC™), New Immunoassay Products Immunoassay New FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. IMMUNOASSAY PLATFORMS

NEW KITS 4 |SigmaAldrich.com/milliplex For Research UseOnly. NotFor UseInDiagnosticProcedures. critical vaccine development, epidemiology, andpopulationresearch studies. serum orplasmasamples. Theseimmunoassay canassistinCOVID-19 kits for research efforts • • • MILLIPLEX Multiplex your COVID-19 research discoveries with have developed MILLIPLEX COVID-19 (SARS-CoV-2). Inresponse totheneedforSARS-CoV-2-specific immunoassay kits, we We are toproviding committed researchers with reliable resources andtoolstounderstand MILLIPLEX 1  mechanically ventilated COVID-19 positive humanserum/plasmasamples, indicatingasuppressive roleforIL-10. frompatientsamples. thesedata obtain a.Anti-S1, b. S2,c.RBD, andd. NIgG antibodiescorrelated negatively with IL-10 in Figure. MILLIPLEX 2020.12.05.20244541; doi:https://doi.org/10.1101/2020.12.05.20244541 IgG Antibodiesagainst SARS-CoV-2 Correlate withDays from SymptomOnset,ViralLoad andIL-10. Young, etal.medRxiv understanding autoimmunity related autoimmunity toSARS-CoV-2understanding infection. MILLIPLEX analysis inCOVID-19. MILLIPLEX (RBD), andnucleocapsidprotein(N). IgM, andIgA toSARS-CoV-2 antigens:spike S1andS2,receptor proteinsubunits binding domain MILLIPLEX at SigmaAldrich.com/milliplex-covid-19 Learn more abouthowMILLIPLEX ® ® ® HumanAutoimmuneAutoantibodyPanel andHumanCytokine AutoantibodyPanel for Factor Multiplex48-plexHumanCytokine/Chemokine/Growth Panel Aforcytokine SARS-CoV-2 AntigenPanels IgG, fordetectionofhumanimmunoglobulinisotypes ® SARS-CoV-2 AntigenPanel 1IgG Factor andHumanCytokine/Chemokine/Growth Panel Awere usedto ® immunoassays usingtheLuminex ® Panels forCOVID-19 Research ® assays thatdetectantibodiesagainstSARS-CoV-2 antigensinhuman ® multiplexassays COVID-19 are beingusedtounderstand ® platform. 1 SMC™ (SINGLE MOLECULE COUNTING) ASSAYS ™

SMC Technology SMC™ PLATFORM Single Molecule Counting (SMC™) Technology: Reduced background + increased signal

SMC™ technology provides maximum to-noise ratios over traditional immunoassay immunoassay performance while following a technologies. The SMC™ technology thus workflow similar to traditional ELISA technology, provides enhanced quantification at both low as shown below. By combining a unique assay and high levels of expression on one complete elution step and robust digital counting, system. SMC™ technology achieves improved signal-

STANDARD PROPRIETARY

12Analyte of Interest in Capture on 3 Detect 45Elute Quantify Solution Plate or Bead Complex is chemically Sample is detected by laser; broken apart detection tags counted

Steps 2-3 Step 4 Step 5 Antibodies translate each Fluorescent dye-labeled On the SMCxPRO™ biomarker into a signal detection antibodies instrument, signal is dissociate from the collected from free-floating immunocomplex fluorochromes using a rotating laser, camera, and counting individual molecules by an Avalanche Photodiode

Digital counting improves sensitivity and dynamic range

®

The SMCxPRO™ and Erenna instruments capture the sum of all digital events counted above the FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. background threshold. The digital event algorithm of the SMCxPRO™ instrument computes digital events across a spectrum of time series for a single standard. On the Erenna® instrument, at lower concentrations, photons are digitally counted as single molecules. At higher concentrations, molecules are counted in analog and a proprietary algorithm computes the total sum of all photons recorded. The digital counting of molecules thus improves the assay sensitivity and extends the assay dynamic range beyond what can be achieved with traditional methods.

SigmaAldrich.com/smc | 5 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING) TECHNOLOGY

SMC™ PLATFORM SMC 9 8 7 6 5 4 3 2 1 For Research UseOnly. NotFor UseIn Diagnostic Procedures. 6 |SigmaAldrich.com/smc Qualitative assay Samplevolume varies basedondilutionfactorselectedby user Streamlined formatkit. Assay isrepresentative characteristicdata forbothErenna Plate-basedassay.   baselinebiomarker toquantify assessedforability MedianEndogenous:Minimumof 10samplesfromindividualdonors values. <20%andaccuracywithin 20%ofexpectedvalues. curve withCV LLoQ:Lowestpointonstandard Glucagon IL-10 GLP-1 (total) TNFα IL-17A/F Heterodimer IL-8 /CXCL8 IL-22 IFN-γ IFN-α2 GLP-1 (active) SARS-CoV-2 RBDIgG VEGF-A UCHL1 TSLP TNFα Phospho-Tau(Thr181) MNPTX2 MCP-1 KIM-1 IL-18 IL-17F IL-17A IL-15 IL-13 IL-12p70 IL-7 IL-6 IL-5 IL-4 IL-23 IL-21 IL-2 (newversion) IL-1β IL-1α2 IFN-β1 GM-CSF G-CSF cTnI BDNF Amyloid 1-42 beta Amyloid 1-40 beta Total Akt1 Phospho-Akt1 (Ser473) Total-α-Synuclein Synuclein(Ser129) Phospho-α- SARS-CoV-2 S1IgA Analyte Optimized for use in sample type(s) listed.Key:Optimized foruseinsampletype(s) P=Plasma;S=Serum;L=Lysate; U=Urine;C=Cerebrospinal Fluid(CSF) Key: H=Human; M=Mouse;R=Rat;GP=GuineaPig;Cy=Cynomolgus Monkey; C=Canine type listed.Otherlistedspecieshave species Optimized forfirst beentested,butnotoptimizedforpeakperformance. 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7 5 6,7 6,7 6,7 6,7 6,7 6,7 6,7 6,7

6,7 6,7 ™ NEW Immunoassay Kits 6,7 6,7 6,7

6,7

NEW NEW 6,7 6,7 NEW 0.39 0.24 0.40 NA 0.40 0.10 0.39 0.20 0.15 0.02 0.08 7.80 0.98 NA 0.20 2.60 1.56 0.20 0.94 0.58 0.15 3.91 0.09 0.20 0.07 1.17 0.04 0.05 0.10 0.08 0.04 0.04 0.10 0.10 0.09 0.20 0.12 0.03 0.031 0.78 0.69 0.04 0.98 5.86 1.95 0.1 (pg/mL) LLoQ 9 9 1 17.8 3.6 3.46 38.6 3.38 4.91 0.53 P: 0.35;S:0.64 0.2 17 NA NA P: 48;S:50 P: 22.06;S:20.49;C:413 P: 5.04;S:3.86 P: 0.85;S:0.78 P: 3.4;S:2.0;C:21 P: 8268;S:8161;C:1677 P: 682;S:1021 P: 65;S:75;U: 147 P: 138;S:145 P: 1.10;S:1.18 P: 0.31;S:0.30 P: 1.36;S:3.20 P: 0.37;S:0.33 P: 0.29;S:0.28 P: 0.90;S:0.89 P: 0.70;S:0.69 P: 0.37;S:0.30 P: 0.18;S:0.18 P: 0.65;S:0.46 P: 1.51;S:1.52 0.21 P: 0.11;S:0.12 P: 0.09;S:0.10 P: 1.4;S:1.4 P: 0.08;S:0.08 44 P: 3.04;S:3.02 P:783; S:410;CSF: 0.13 Normal CSF: 192;P:15 Normal CSF: 1366;P:152 P: 1885;S:1240;C:233 P: 2.74;S:1.81;C:0.64 NA NA Endogenous (pg/mL) Median 9 9 ® andSMCxPRO™ instruments. 2 H, M,R,C H H, M,R,C M H H H H H H H, M,R H, M,R H H H H H H H H H H H H H H H H H H H H H H H H H H, M,R H, Cy, R,C,GP H H, M,R H, M,R H H H H Species 3 P ,S P, P S S P, S P, S S P, P P L L ,S P, P, S, C S P, S P, P, S, C P, S, C S P, P, S, U S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, S P, P S P, C, P, S C, P C, P P, S, C P, S, C ,S P, S P, Type Sample 4

20 50 100 25 100 50 50 50 100 10 varies varies 25 25, 5 100 100 50, 100,25 0.1, 0.3 1.56 25, 5 10 50 50 50 100 100 100 75 100 100 100 25 100 100 100 25 100 10 100 100, 1,1 5, 25 5, 25 0.5, 10 20, 50 2 2 volume (μL) Sample 8 8 MTO MTO MTO MTO MTO MTO MTO STOCK MTO MTO STOCK STOCK MTO STOCK STOCK STOCK STOCK MTO STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK MTO MTO STOCK STOCK STOCK STOCK STOCK MTO or 03-0025-06 03-0055-00 03-0024-03 03-0108-00 03-0058-00 03-0094-00 03-0014-07 03-0191-00 03-0067-00 03-0047-00 03-0180-00 03-0183-00 03-0172-00 03-0163-00 03-0184-00 03-0199-00 03-0187-00 03-0118-00 03-0190-00 03-0164-00 03-0159-00 03-0177-00 03-0156-00 03-0182-00 03-0176-00 03-0155-00 03-0168-00 03-0161-00 03-0157-00 03-0162-00 03-0195-00 03-0160-00 03-0167-00 03-0181-00 03-0186-00 03-0153-00 03-0154-00 03-0171-00 03-0146-00 03-0145-00 03-0099-01 03-0100-01 03-0196-00 03-0188-00 03-0197-00 03-0193-00 Cat. No.Cat. SMC™ (SINGLE MOLECULE COUNTING) ASSAYS

Mouse Plate-Based Discovery SMC™ PLATFORM Immunoassay Kits Discovery immunoassays are provided as complete kits to run on your Erenna® platform

• Reproducible LLoQ • Simple workflow • Minimal sample volume

LLoQ Median Endogenous Analyte (pg/mL) (pg/mL) Species Sample Type Cat. No. IL-5 0.24 6.1 M S 03-0132-00 IL-10 6.2 8.3 M S 03-0134-00 IL-13 3.91 12.0 M S 03-0133-00 IL-17A 0.98 9.2 M S 03-0123-00 IL-17F 0.49 2.2 M S 03-0125-01 IL-21 4.9 44.0 M S 03-0126-00 IL-22 100 195 M S 03-0127-00 TNFα 0.49 28.4 M S 03-0137-00

Aurora High-Performing Microplates for Use on the SMCxPRO™ System

Partnering with Aurora Microplates, Inc., we are the exclusive distributor of the High-Performance 384-Well Microplate- specifically packaged for use on the SMCxPRO™ platform. Enhanced detection and stability of your biological materials are critical for generating consistent, high-quality data. Containing a flat-bottom

design with a high transmittance window, Aurora FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. plates are optimized for imaging. They are also available in two convenient package sizes. Plates are now bar-coded to enable 100% traceability of each plate to all raw materials and 100% audit ability for ISO or other traceability requirements. The bar code label can also be used in automated systems for plate tracking, data management, and plate orientation.

Description Cat. No. 384-well Bar-coded Aurora plate ABB2-10160A (Case of 32 plates) 384-well Aurora plate (Case of 32 plates) ABB2-00160A

One 384-well Aurora plate w/ 3 plate seals 02-1008-00

To learn how SMC™ technology can advance your drug discovery research, visit EMDMillipore.com/smc-immunogenicity

SigmaAldrich.com/smc | 7 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING) ASSAYS

SMC™ PLATFORM Use Only. NotFor UseInDiagnosticProcedures. evaluation ofCOVID-19 effectorimmunity. For Research while ourextensive assays SMC™cytokine canbeusedfor infected individualsas wellasfromvaccine trialenrollees, insamplesfrom characterization ofhumoralimmunity SMC™ SARS-CoV-2 RBDIgG sensitive Kittofacilitate CoV-2 S1IgA ourpreviously Kitcomplements released to continuethesevaliant efforts. OurnewSMC™SARS- immunoassay withpowerful equip scientists toolsthey need pandemic. Meanwhile, wehave to continuedourefforts ofthenovel bolsterourunderstanding further coronavirus emerging vaccines, characterizenewviralvariants, and quest toconductcriticalSARS-CoV-2 studiestoevaluate the world,globalresearch continuesits community With COVID-19 vaccination activitieshappeningaround critical COVID-19 research New SMC™SARS-CoV-2 S1IgA Kit:Anothertooltoempower (SMC™) technology. unparalleled powerofSingleMoleculeCounting more effectively than ever before, usingthe IL-2 inpharmacologicalandclinicalresearch enable researchers tothoroughlycharacterize verification, andastreamlined assay protocolto curve range,standard thoroughin-house combines improved assay sensitivity, greater Human IL-2 Immunoassay HighSensitivity Kit research.and autoimmunity ThenewSMC™ for decades, withapplicationsinoncology of interest intranslationaldiseaseresearch and regulatory cells, IL-2 hasbeenamolecule impacting T-cell differentiation intomemory adaptive immuneresponse. Withfunctions in activation andregulation ofantigen-specific CD4+ andCD8+Tlymphocytes, andfunctions thatisprimarilyproducedbycytokine activated Human Interleukin-2(IL-2) isasignaling research immuno-oncology andautoimmunity New andimproved SMC™HumanIL-2 assay for SMC For Research UseOnly. NotFor UseInDiagnosticProcedures. 8 |SigmaAldrich.com/smc SMC™ HumanIL-4 Immunoassay HighSensitivity Kit ImmunoassaySMC™ HumanIFN-α2HighSensitivity Kit SMC™ HumanTNF-α Immunoassay HighSensitivity Kit SMC™ HumanIL-6 Immunoassay HighSensitivity Kit SMC™ SARS-CoV-2 RBDIgM Kit SMC™ SARS-CoV-2 S1IgA Kit SMC™ SARS-CoV-2 RBD IgG Kit Description SMC™ HumanIL-2 Immunoassay HighSensitivity Kit Description ™ NewProductHighlights Coming Soon Coming 03-0161-00 03-0186-00 03-0163-00 03-0155-00 03-0197-00 03-0193-00 03-0195-00 Cat. No.Cat. No.Cat. SMC™ (SINGLE MOLECULE COUNTING) ASSAYS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CUSTOM 21/05/19 9:53 PM Cat. No. 02-1008-00 03-0178-00 03-0179-00 96-2024-00 96-2025-00 03-0078-00 03-0128-00 03-0076-02 03-0077-02 03-0122-00 03-0175-00 ABB2-10160A ABB2-00160A SigmaAldrich.com/smc | 9 SigmaAldrich.com/smc Analyze and discover Discover 3 10 Contact your local MilliporeSigma local MilliporeSigma Contact your ask how our kits and Representative money. time and you can save Name Phone number Email address -2 10 2 n -1 10 -0 10 Using SMC™ technology combined with our Using SMC™ technology combined development unique immunogenicity assay a homogenous develop can kit, researchers that is species independent bridging assay The SMC™ to design, and verify. easy simple, development Immunogenicity bead-based assay and to develop need you kit includes everything assay The comprehensive assay. an ADA verify and protocol is designed to guide development Our experienced with the process. assist you application scientists can also help design, and also train end-users to fully utilize verify, the platform. Homebrew Assay with Homebrew Q1: Standard Group -3 Expected Concentratio 10 ™ Test EP Signal vs. Expected Concentration -3 10 Verify your assay your Verify -3 10 4 8 7 6 5 10 10 10 10 10

384-well Aurora Plate (Case of 32 plates) One 384-well Aurora plate w/ 3 plate seals SMC™ Bead-based Assay Development Kit (5 plates) Development SMC™ Bead-based Assay Kit (5 plates) Development SMC™ Plate-based Assay – 3 days Training Development SMC™ Assay – extra day Training Development SMC™ Assay Kit Development SMC™ Bead-based Immunoassay Kit Development SMC™ Plate-based Immunoassay SMC™ Detection Labeling Kit Labeling Kit SMC™ Capture Buffer Optimization Kit SMC™ Assay Kit Development SMC™ Immunogenicity Bead-based Assay Aurora Plate (Case of 32 plates) 384-well Bar-coded Description l Signa EP System. ® Technology to Accelerate Your to Accelerate Your Technology Better ™ Sensitivity Custom high-sensitivity assay development is as easy as … is as easy Custom high-sensitivity development assay Design Assays Simplistic Label Personalized Conjugate your antibodies Conjugate your

Reliable Better Sensitivity..... Femtogram/mL Better Sensitivity..... Improve your homebrew assays with SMC™ assay development kits development with SMC™ assay assays homebrew your Improve

Field Support EMDMillipore.com/smctech The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. in the U.S. operates as MilliporeSigma Darmstadt, Germany science business of Merck KGaA, The life are and SMC the vibrant M, Millipore, MilliporeSigma, Reserved. All Rights and/or its Darmstadt, affiliates. Germany © 2019 Merck KGaA, Detailed owners. are the property respective All other trademarks of their or its Darmstadt, affiliates. Germany trademarks of Merck KGaA, via publicly accessible resources. information on trademarks is available 1.0 2019 - 19901 05/2019 MS_FL3801EN Ver. Lit. No. Our SMC™ assay development kits provide everything you need to develop your own femtomolar-range own femtomolar-range your need to develop you everything kits provide development Our SMC™ assay for use with the SMCxPRO immunoassay Detecting negative immunogenic responses immunogenic responses Detecting negative process is critical early in the drug discovery are responses potential immune to ensure Informed decisions can then identified promptly. effective be made as companies seek to develop clinical efficacy and improved with medicines, Assessing to critical diseases. patient safety, the immunogenicity occurs of a compound via (ADA), the detection of anti-drug antibodies guidelines IgE. FDA IgM, and IgG, specifically importance the continue to stress of high sensitivity of a as this leads to earlier detection for which is essential primary immune response patient safety. 1:1 Training and Customized Kits Customized and 1:1 Training Take advantage of our ultrasensitive Single of our ultrasensitive advantage Take technology whileCounting (SMC™) Molecule own application-specific your developing in either bead- or plate-basedimmunoassays and optimization development format. Our assay need to build your you everything kits provide for useown high performing SMC™ immunoassay Use our platform tosystem. with the SMCxPRO™ to accelerate your required customize the assay development assay offer hands-on We research. facility or platform at your training for the SMC™ centers. one of our global training Develop your own SMC own your Develop Immunogenicity Studies Utilize SMC 2019 - 19901_RS_IPS SMC HomeBrew- Flyer Version 2_update_MSIG.indd 1 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING) CUSTOM ASSAYS AND SERVICES

CUSTOM 10 |SigmaAldrich.com/smc • • • Biotherapeutic Development • • • & Development Collaborative Biomarker Discovery include: Our services for you. accelerate your programfromdiscovery research intoclinicaltrialswithacustomdesignedprogram immunoassay development experiencewithcore inSMC™technology. expertise Letushelpyou protein ofinterest ortotestyour criticalsamples. teamhasover OurCAST 130years ofcombined We you don’t understand always have thetimeorresources todevelop your ownassay foryour forSMC (CAST) CustomAssays &SampleTestingExpert immunoassays. samples usingoneofourhighlyverified SMC™ Sample testingofupto30your critical Sample Testing: SMCxPlore™ ProofofConcept your assay inhouse. Ideal andstandard. antibody pairs forbringing Development assay ofaprototype usingyour Feasibility: SMCxPlore™ Assay Development technology withyour ofinterest analyte orcriticalsamples. Utilize ourSMCxPlore™ thatincludecustomizedstreamlined Services optionsto“explore” SMC™ Immunogenicity Pharmacodynamics Pharmacokinetics Biomarker verification tosponsor requirements Immunoassay development Preclinical toearlyclinicalsampletesting ™ Technology • • • Sample Testing • • • • Reagent Selection available. let usdevelop aSMC™assay thatisn’t currently With accesstoover 600proven antibodypairs, SMC™ Assay: SMCxPlore™ to Assay Conversion SMC™ immunoassay kittesting kitsampletesting Proprietary sampletesting Prototype QC generation Antibody derivatization Screening andselection Reagent sourcing SMC™ (SINGLE MOLECULE COUNTING) CUSTOM ASSAYS AND SERVICES FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

SMCxPRO™ PLATFORM 1 1 2 <1 fM >4 logs <10% CV Specification Cat. No. <3 response/fM 95-0100-00 96-2011-00 96-2022-00 96-2023-00 96-2012-00 96-2013-00 95-0004-05 70-0009-00 >1.2 response/fM SigmaAldrich.com/smc | 11 SigmaAldrich.com/smc Preventative maintenance and repair service maintenance and repair Preventative or less of 5 days turn-around Average Daily international shipping Unlimited visits including labor, Service: Unlimited visits including labor, Total all parts and service for any expenses, travel, for one year and travel, Service:Advanced Labor, maintenance expenses for one preventative year and one floating visit for one Essential Service: Labor for one preventative maintenance visit for one year Determined from calibrator set: 0-300 fM of 150 kD antibody labeled with fluorophore Slope Precision Metric Background Limit of Detection (LoD) Dynamic Range  n=20 from 30fM calibrator, measurements Response We now offer the option to ship your instrument now offer the option to ship We This includes: to our depot center for service. • • • Three Levels of Onsite Service of Onsite Levels Three Support Platform for SMCxPRO™ • • • Minimum Instrument Performance Specifications 1 2 ServiceDepot Repair Now Available 405 Touch Microplate Washer with MAG plate with MAG Microplate Washer 405 Touch ® SMCxPRO™ Complete System Complete SMCxPRO™ SMCxPRO™ Platform & Ancillary Product ServicesSMCxPRO™ Installation Standard SMCxPRO™ day – 1 Training System SMCxPRO™ – 3 days Training SMC™ Advanced Installation IQ/OQ SMCxPRO™ PQ Service SMCxPRO™ BioTek Jitterbug™ Plate Shaker Jitterbug™ Immunoassay System and Services and System Immunoassay 7/10 Operating Systems ® ™ Software is 21CFR part II compliant and LIMS compatible Run ASSIST program to verify instrument program to verify ASSIST Run performance Optional IQ/OQ and PQ Services available Set up of unit on bench and complete calibration of unit Instrument dimensions: 406.4 mm H x 355.6 mm W x 444.5 mm D (16"H x 14"W x 17.5"D) (50 lbs) 22.7 kg Weight: 50-60 Hz US: 115 VAC, (Operating range 90-125 V) 50-60 Hz International: 230 VAC, (Operating range 180-250 V) Bead-based assays Plate-based assays 384-well plate Microsoft Windows A static IP address and an FTPA static IP address server Software Operating and Analysis xPRO included (32- or 64-bit compliant) • • • Installation and Training • • • • Instrument Specifications Assay Format • • Read Plate Format Read • Network/PC Requirement • The SMCxPRO™ immunoassay system is a compact affordable system for ultrasensitive biomarker biomarker for ultrasensitive system is a compact affordable system immunoassay The SMCxPRO™ plates to quantify 384-well at protein in samples is designed to read The instrument detection. design has no The technologies. current than ordersmagnitude greater of three concentrations required. sensitivity ultra-high is where solution maintenance affordable making this a low fluidics SMCxPRO • • FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING) EQUIPMENT

SMC™ PLATFORM 12 |SigmaAldrich.com/smc Ancillary Equipment toOptimizeyourAncillary SMC SMC BioTek Boekel ScientificJitterbug ™ PlateWasher Evaluation Kit ® 405 ™ TSUVSMagnetic96-wellWasher forSMC ™ , 130000,HeatedMicroplateShaker (115V) use andglove touchablescreen. protocols. aneasy-to- The405™TSUVSmodeloffers and customizedpre-programmed plate washing the inclusionofaspeciallydesignedmagneticplate specifically optimizedforusewithSMC™assays with washing, andvacuum filtration.The system comes entire platethroughbiomagneticseparation, automated system designedtoquicklywashan latest advancements in96-wellplatewashing:afully In partnership with BioTekIn partnership risk ofcontamination. includes aninnerlidtoeliminatelightandreduce the ideal forimmunoassays like SMC™assays. It The microplateshaker istemperature controlledand your assay withconfidence. ofyourassess theperformance platewasherandrun with anabbreviated workflow. Thisallows you to plates, mimickingstepsintheSMC™immunoassay This kitprovides allrequired reagents torun5 washer usingourSMC™PlateWasher Evaluation Kit. Evaluate ofyour theperformance SMC™ plate Ultrasonic CleaningforSMC™Platform Washer CompletewithTouch Screen and BioTek Description Microplate Shaker (230V) Boekel ScientificJitterbug™ Heated Microplate Shaker (115V) Boekel ScientificJitterbug™ Heated Description SMC™ PlateWasher Evaluation Kit Description ® 405™TSUVSMagnetic96-well ® instruments, weofferthe ™ Platform ™ Assays 70-0009-00A 95-0004-05 70-0009-00 03-0165-00 Cat. No.Cat. Cat. No.Cat. No.Cat. ELISA ®

Millipore Brand ELISAs s Metabolic/Endocrine/Cytokine Our menu of metabolic, endocrine, and cytokine ELISAs gives end-users a consistent and reliable method of investigating metabolic disease states or defining outcomes within toxicological studies during drug development.

Standard Sample Bulk Packaging Description Species Curve Range Sensitivity Volume Cat. No. Cat. No.* Adiponectin Human 1.56–200 ng/mL 0.2 ng/mL 10 µL EZHADP-61K EZHADP-61BK Adiponectin (High Human 1.5–200 ng/mL 0.5 ng/mL 10 μL EZHMWAN-65K Molecular Weight) Adiponectin Mouse 1–50 ng/mL 0.2 ng/mL 10 µL EZMADP-60K EZMADP-60BK Adiponectin Rat 3.125–200 ng/mL 0.4 ng/mL 10 µL EZRADP-62K Amylin (active) Human 1–100 pM 0.7 pM 50 µL EZHA-52K C-Peptide Human 0.2–20 ng/mL 0.05 ng/mL 10 µL EZHCP-20K EZHCP-20BK C-Peptide Canine 0.2–10 ng/mL 0.24 ng/mL 25 µL EZCCP-47K C-Peptide 2 Mouse, Rat 25–1600 pM 15.0 pM 20 µL EZRMCP2-21K C-Reactive Protein (CRP) Human 0.12-10 ng/mL 0.2 ng/mL 2 μL CYT298 C-Reactive Protein (CRP) Rat 4.9-10 ng/mL 2.5 ng/mL 2 μL CYT294 FGF-21 Human 31.25–2000 pg/mL 10.0 pg/mL 50 µL EZHFGF21-19K FGF-21 Mouse, Rat 49.4–12,000 pg/mL 10.0 pg/mL 10 µL EZRMFGF21-26K FGF-23 Human 9.9–2400 pg/mL 3.5 pg/mL 50 µL EZHFGF23-32K EZHFGF23-32BK Ghrelin (active) Human 25–2000 pg/mL 15.0 pg/mL 20 µL EZGRA-88K EZGRA-88BK Ghrelin (active) Mouse, Rat 25–2000 pg/mL 8.0 pg/mL 20 µL EZRGRA-90K Ghrelin (total) Human 100–5000 pg/mL 50.0 pg/mL 20 µL EZGRT-89K EZGRT-89BK Ghrelin (total) Mouse, Rat 0.1–10 ng/mL 0.04 ng/mL 20 µL EZRGRT-91K GIP (total) Human 8.2–2000 pg/mL 4.2 pg/mL 20 µL EZHGIP-54K EZHGIP-54BK GIP (total) Mouse, Rat 8.2–2000 pg/mL 4.2 pg/mL 10 µL EZRMGIP-55K EZRMGIP-55BK GLP-1 (active) Multi-Species 2–100 pM 1.0 pM 100 µL EGLP-35K EGLP-35BK GLP-1 High Sensitivity Multi-Species See data sheet 0.14 pM 50 μL EZGLPHS-35K EZGLPHS-35BK (active) ∆•• GLP-1 (total) Multi-Species 4.1–1000 pM 1.0 pM 20-50 μL EZGLP1T-36K EZGLP1T-36BK GLP-2 Multi-Species 1–64 ng/mL 0.3 ng/mL 50 µL EZGLP2-37K Glucagon ∆ Human, 0.02–2 ng/mL 0.003 ng/mL 150-300 EZGLU-30K EZGLU-30BK

Mouse, Rat μL FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Growth Hormone (GH) Mouse, Rat 0.7–50 ng/mL 0.07 ng/mL 10 µL EZRMGH-45K EZRMGH-45BK

IL-6 NEW Human 1.17–150 pg/mL 1.17 pg/mL 50 μL EZIL6-98K EZIL6-98K10PK

IL-8 NEW Human 0.82–200 pg/mL 0.8 pg/mL 50 μL EZIL8-100K EZIL8-100K10PK ELISA Insulin Human 2–200 µU/mL 1.0 μU/mL 20 μL EZHI-14K EZHI-14BK Insulin Mouse, Rat 0.2–10 ng/mL 0.1 ng/mL 10 μL EZRMI-13K EZRMI-13BK s Insulin (Animal Human 2–200 µU/mL 0.85 μU/mL 20 μL EZHIASF-14K serum free) Leptin Canine 0.78–50 ng/mL 0.21 ng/mL 20 µL EZCL-31K Leptin Mouse 0.2–30 ng/mL 0.05 ng/mL 10 µL EZML-82K EZML-82BK Leptin Rat 0.2–30 ng/mL 0.08 ng/mL 10 µL EZRL-83K EZRL-83BK Leptin “Dual Range” Human 0.5–100 ng/mL 0.2 ng/mL 25 µL EZHL-80SK EZHL-80BK

MCP-1 NEW Human 7.18–1000 pg/mL 7.18 pg/mL 10 μL (1:5 EZMCP1-99KRM EZMCP1-99K10PKRM dilution) Procollagen Type IIA Human lot dependent 30.0 ng/mL 5 µL EZPIIANP-53K N-Propeptide (PIIANP) Proinsulin (total) Human 2–200 pM 0.5 pM 20 µL EZHPI-15K EZHPI-15BK PYY (total) Human 10–2000 pg/mL 6.5 pg/mL 20 µL EZHPYYT66K Resistin Human 0.16–5 ng/mL 0.02 ng/mL 20 μL EZHR-95K EZHR-95BK SAA-3 Mouse 0.078-5 μg/mL 0.078 μg/mL 10 μL EZMSAA3-12K

* Bulk packaging available on select kits — more environmentally friendly and saves space (10 kit equivalent) •• Preferred assay for measuring GLP-1 (active) ∆ Chemiluminescent assay

SigmaAldrich.com/elisa | 13 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ELISAs AND RIAs

ELISA / RIA 14 |SigmaAldrich.com/elisa system withreliable quantificationofbiomarkers usingourneuropeptideandneurodegenerative ELISAs. Get acompletepicture ofthenervous ofthecomplexitiesassociatedwithnormalanddiseasestates Neuroscience ELISAs Millipore using our other high-quality immunoassaysusing ourotherhigh-quality foryour research needs. concentration. Asmany researchers move away fromusingradioactive material,pleaseconsider RIAs have longbeenconsidered avaluable, economical,andaccurateway tomeasure protein Radioimmunoassays (RIAs) S100B (pNF-H) Sandwich Neurofilament, Phosphorylated Derived Factor) PEDF (PigmentEpithelium- NPY (NeuropeptideY) NPY (NeuropeptideY) Acidic Protein) GFAP (GlialFibrillary PYY (total) Leptin Insulin GLP-1 (total) GLP-1 (active) Neurotrophic Factor) BDNF (Brain-Derived Amyloid (Brain),Set beta Amyloid (Brain)1-42 beta Amyloid (Brain)1-40 beta Amyloid Set beta, Amyloid 1-42 beta Amyloid 1-40 beta PYY (3-36) Proinsulin Leptin Insulin Specific Insulin (sensitive) Insulin LisPro Insulin Glucagon Ghrelin (total) Description C-Peptide Adiponectin Description Ghrelin (active) C-Peptide ® Brand ELISAs &RIAs BrandELISAs Human Human Human Multi-Species Human Human Rat Multi-Species Rat Porcine Multi-Species Multi-Species Multi-Species Human Human Human Canine Human Species Human Multi-Species Human Mouse, Rat Human Human, Mouse, Rat Human, Rat Human Human Human Human Human Human Species 0.156–20 ng/mL 2.5–250 µU/mL 0.5–100 ng/mL 20–400 pg/mL 0.02–1 ng/mL 0.1–10 ng/mL lot dependent lot dependent lot dependent lot dependent lot dependent 2–200 µU/mL 2–200 μU/mL Curve Range Curve 0.1–5 ng/mL 10–1000 pM 1–50 ng/mL 10–500 pM 2–100 pM Standard Standard 0.0293–15 ng/mL 2.7-2000 pg/mL 0.9–62.5 ng/mL 7.8–500 pg/mL 1.5-100 ng/mL 16–500 pg/mL 16–500 pg/mL 16–500 pg/mL 16–500 pg/mL 5-1000 pg/mL 0.01-2 ng/mL Contains 1eachofEZBRAIN40andEZBRAIN42 Contains Curve Range Curve Contains 1eachofEZHS40andEZHS42 Contains Standard Standard

0.02 ng/mL 0.15 ng/mL Sensitivity 2.5 µU/mL 0.5 ng/mL 0.1 ng/mL 0.1 ng/mL 7.8 pg/mL 0.0585 ng/mL 10 pg/mL 20 pg/mL 20 pg/mL 93 pg/mL 0.004 ng/mL 2 µU/mL 2 μU/mL 1 ng/mL 1 ng/mL Sensitivity 0.9 ng/mL 1.5 ng/mL 1.3 pg/mL 7.8 pg/mL 3 pM 2 pM 3 pM 2 pg/mL 5 pg/mL 4 pg/mL 5 pg/mL 4 pg/mL 100–200 µL 50–100 µL <100 µL <100 µL Volume Sample 300 µL 100 µL 300 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 μL 5 µL Volume Sample 1-10 µL 100 μL 50 µL 50 µL 50 µL 20 µL 50 µL 50 µL 50 µL 50 µL 50 µL EZHS100B-33K EZRMNPY-27K EZBRAIN-SET GLP1A-35HK GLP1T-36HK GHRA-88HK EZHNPY-25K HADP-61HK GHRT-89HK PYYT-66HK EZBRAIN42 EZBRAIN40 CCP-24HK PYY-67HK EZHS-SET HCP-20K Cat. No. Cat. HPI-15K Cat. No. Cat. SRI-13K LPI-16K GL-32K HL-81K XL-85K HI-14K RI-13K EZHS42 EZHS40 PI-12K CYT420 CYT306 NS170 NS830 SEARCH OUR ELISA Sigma-Aldrich® Brand ELISAs We offer a portfolio of > 1,800 ELISAs and EIAs, covering a diverse range of species and sample types. A variety of research areas are addressed, from inflammation and cardiovascular disease to metabolic and veterinary research. With such a large number of ELISAs, it is impossible to list them all on a single page, so search your target at SigmaAldrich.com/elisa. To view protocols and performance parameters, type “sample” into the Certificate of Analysis box and open the PDF. s ONLINE

ELISA Designations • Sandwich ELISA • EIA – Standard off-the-shelf assay – Competitive ELISA – Some may be designated “lysate” to • Cell-Based Phosphorylation ELISA accommodate the change from serum, – For use on cell lysates to detect plasma, or supernatant phosphorylation events • Custom Sandwich ELISA – Requires custom manufacture and validation – Upcharge may apply

Cytokines Metabolic Cardiovascular Angiogenesis Signaling ✔ ✔ ✔ ✔ ✔

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✔ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ✔ ✔ ELISA ✔ s

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SigmaAldrich.com/elisa | 15 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ELISA READER / WASHER

BIOTEK® WASHERS 16 |SigmaAldrich.com/elisa Supporting ourcollectionofoverSupporting 1,800ELISAproducts, weare pleasedtointroducetheBioTek withBioTekPartnership ELISA Reading andWashing Equipment in your ELISAworkflow. TS AbsorbanceReader and50TSPlateWasher whichtogetherprovide acost-effective addition to For Millipore BioTek BioTek BioTek ® 50TSMagnetic96-wellAutomatedStripWasher ® ® 50TSPlateWasher 800TSAbsorbanceReader ® andSigma-Aldrich ® ELISAs • • • • • • • • • • • • • BioTek Gen 5Software BioTek Gen 5Secure BioTek Description BioTek Description Strip Washer Durable and high-quality design Durable andhigh-quality USB flashdrive for convenient export data simple operation Color touchscreen forquickprogrammingand Optional absorbancetestNISTtraceableplate 750nm asstandard Flexible filteroptions;450nm,590nm,630nm,and Software options:Basic,Gen5,and5Secure cell-based assays other end-pointassays, pluskineticsand applications, includingELISA,proteinand Precise, accurate, andversatile, usedformany Replaces theBioTek for continuedreliable operation Automated, built-in maintenanceroutines even greater automation for Automated switchingofupto3buffers Reliable andsafe: liquidlevel sensing plates partial Easy touch operationforwashingfullor and easy Color touchscreen makes programmingquick ® ® ® ® ® 50TSPlateWasher 800TSAbsorbanceReader, 800TSAbsorbanceReader, Basic 800TSAbsorbanceReader, Instruments ® ELx50Magnetic96-well Cat. No.Cat. No.Cat. 40-301 40-006 40-300 40-007 ® 800 MILLIPLEX ® MILLIPLEX Analyte Species Index MILLIPLEX

Analyte Species Analyte Species Analyte Species ® ANALYTE INDEX 4–6 B7-H6...... H Collagen IV – COMP (cartilage ®

4-1BBL/TNFSF9...... H BAD (total)...... H oligomeric matrix protein)...... H PANELS 4E-BP1 (total)...... H,M,R BAD (Ser112)...... H,M C1q (autoantibody)...... H 4E-BP1 (Thr37/46)...... H,M,R BAFF/Blys...... H Complement C1q...... H 5’-NT/CD73...... H,R BAFF/Blys (autoantibody)...... H Complement C2...... H 6Ckine/CCL21/Exodus-2...... H,M BAX (total)...... H Complement C3...... H BCA-1/CXCL13...... H,NHP Complement C3b/iC3b...... H A Bcl-2 (total)...... H Complement C4...... H α 1-Acid Glycoprotein (AGP)...... H,M,R Bcl-2 (Ser70)...... H Complement C4b...... H α-1-Antitrypsin (A1AT)...... H Bcl-xL (total)...... H Complement C5...... H α-1 Microglobulin...... H Bcl-xL/BAD (interaction)...... H Complement C5a...... H α-2 Macroglobulin (A2M)...... H,M,R BDNF...... H,M,R Complement Factor B...... H α-Fetoprotein (AFP)...... H Betacellulin...... M Complement Factor H...... H α-MSH...... H,M,R BIM...... H Complement Factor I...... H α-Synuclein...... H Blk (Tyr389)...... H Connective Tissue Growth Factor ACTH...... C,H,M,NHP,R BMP-9...... H (CTGF)...... R Active Caspase 8 (total)...... H BNP...... H Contactin-1...... H Active Caspase 9 (total)...... H BRAF (Ser446)...... H,M,R Corticosterone...... R ADAMTS13...... H BRAK/CXCL14...... H Cortisol...... H,Mul Adiponectin...... H,M,R BTLA...... H CRAF (Ser338)...... H,M,R Adipsin/Factor D...... H,M Creatine Kinase Muscle (CKM)...... R Agouti-Related Protein (AgRP)...... H,NHP C CREB (Ser133)...... H,M,R ALK-1 (soluble)...... M c-Jun (Ser73)...... H,M,R CREB (total)...... H,M,R Akt/PKB (Ser473)...... H,M,R c-Kit (panTyr)...... H CTACK/CCL27...... H,M Akt/PKB (total)...... H,M,R c-Kit/SCFR (soluble)...... H CTAG1B/NY-ESO-1 (autoantibody)...... H Akt1 (Ser473)...... H,M,R c-Met/HGFR (panTyr)...... H CTLA-4/CD152...... H Akt1 (total)...... H,M,R c-Myc (total)...... H CXCL6/GCP-2...... H Akt2 (Ser474)...... H,M,R C-Peptide...... H,NHP CXCL16...... H,M Akt2 (total)...... H,M,R C-Peptide 2...... M,R CYFR A21-1...... H Akt3 (Ser472)...... H,M,R C-Reactive Protein (CRP)...... H,M Cystatin C...... C,H,M,R Akt3 (total)...... H,M,R CA125...... H Albumin...... C,H,R CA15-3...... H D ALDH1A1...... H CA19-9...... H D-dimer...... H Amphiregulin...... M Calbindin...... H DKK1...... H,M,R Amylin (active)...... H,M,NHP,R Carbonic Anhydrase 9 (CA9)...... H DPP4...... H Amylin (total)...... C,H Cardiac Troponin I (cTnI)...... R Amyloid beta 1-40 (Aβ 1-40)...... H,M Cardiac Troponin T (cTnT)...... R E Amyloid beta 1-42 (Aβ 1-42)...... H,M Cathepsin D...... H E-Cadherin...... H Angiogenin (ANG)...... H Caveolin-1 (CAV-1)...... R EGF...... H,M,R Angiopoietin-2...... H,M CCL28...... H EGFR/HER1/ErbB1 (soluble)...... H Angiostatin/Kringle...... H CCNB1/Cyclin B1 (autoantibody)...... H EGFR2/Her2/ErbB2 (soluble)...... H FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Angiotensinogen (AGT)...... H CD3ε (panTyr)...... H EGFR3/Her3/ErbB3 (soluble)...... H ANGPTL3...... H CD14 (soluble)...... H eIF-4B (Ser422)...... H,M,R ANGPTL4...... H CD25/IL-2Rα...... H eIF-4E (Ser209)...... H,M,R ANGPTL6/AGF...... H CD27...... H eIF-4G (Ser1108)...... H,M,R Antithrombin III...... H CD28...... H eIF2a (Ser51)...... H,M,R Apelin...... H CD30 (soluble)...... H,M ENA-78/CXCL5...... H Apolipoprotein AI (Apo AI)...... H CD30/TNFRSF8...... H Endocan-1 (ESM-1)...... H,M Apolipoprotein AII (Apo AII)...... H CD31/PECAM-1 (soluble)...... H,M Endoglin...... H,M Apolipoprotein B (Apo B)...... H CD40...... H Endostatin...... H Apolipoprotein CII (Apo CII)...... H CD40L (soluble)...... H,M,NHP Endothelin-1...... H,M Apolipoprotein CIII (Apo CIII)...... H CD44...... H ENO1 (autoantibody)...... H Apolipoprotein E (Apo E)...... H CD80/B7-1...... H Eotaxin/CCL11...... E,H,M,NHP,R ApoE4...... H CD86/B7-2...... H Eotaxin-2/CCL24/MPIF-2...... H APRIL...... H CD137/4-1BB/TNFRSF9 (soluble)...... Eotaxin-3/CCL26...... H Arginase-1 (ARG1)...... H,R ...... H,M,NHP EpCAM...... H ATF2 (Thr71)...... H,M CD226/DNAM-1...... H Erythropoietin (EPO)...... H,M,R ATR (total)...... H CEA...... H ErbB1/EGFR (panTyr)...... H AXL (soluble)...... H CENP-A (Centromere Protein A) ErbB2/HER2 (panTyr)...... H (autoantibody)...... H ErbB3/HER3 (panTyr)...... H B CENP-B (Centromere Protein B) ErbB4/HER4 (panTyr)...... H β-2-Microglobulin...... C,H,M,R (autoantibody)...... H Erk/MAPK 1/2 (Thr185/Tyr187)...... H,M,R β-Endorphin...... H,M,R Chk1 (Ser345)...... H,M,R Erk/MAPK 1/2 (total)...... H,M,R β2-Glycoprotein (autoantibody)...... H Chk2 (Thr68)...... H E-Selectin (soluble)...... H,M,R β -Tubulin (total)...... H,M,R CK-MB...... H Estradiol...... Mul B7-H2/ICOSL...... H Clusterin...... C,H,M,R Extracellular Matrix Protein 1 (ECM1).... H B7-H3/CD276...... H CNTF...... H,NHP B7-H4/VTCN1...... H Collagen IV...... H B7-H5/VISTA...... H

B/Bovine, C/Canine, E/Equine, F/Feline, G/Chicken, H/Human, M/Mouse, Mul/Multi-Species, NHP/Non-Human Primate, O/Ovine, Por/Porcine, R/Rat SigmaAldrich.com/milliplex | 17 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® ANALYTE INDEX

MILLIPLEX® PANELS 18 |SigmaAldrich.com/milliplex FSH Fractalkine/CX3CL1 Follistatin-like Protein 1 (FSTL1) Follistatin (FST) (panTyr) Flt3 Ligand Flt3 ActivationFibroblast Protein (FAP) Fibrinogen (Tyr412) Fgr FGL1/Hepassocin GROα B...... Granzyme A...... Granzyme Granulysin Fyn (Tyr420) FGFR1 (panTyr) FGF-23 FGF-21 FGF-19 GSTα GSK3β (Ser9) (total)GSK3β GSK3α (Ser21) (total)GSK3α ...... KC/GRO/CINC-1/CXCL1 GP130 (soluble) GOT1 GnRH GM-CSF Glucagon (total) GLP-1 GLP-1 (active)...... (GFAP)...... Protein Acidic Fibrillary Glial GITRL GITR GIP (total) GIP (active) ...... Ghrelin GH GDNF GDF15 GDF-11 FGF-2/FGF-basic G-CSF (total) GAPDH (GAL3)Galectin-3 (autoantibody) G-CSF (autoantibody)G-CSF FGF-1/FGF-acidic A...... Fetuin Ferritin (soluble)Fas/TNFRSF6 (sFasL) (soluble) ...... Ligand Fas Galectin-3 (Ser194) FADD HCGβ HCC-4/CCL16 HCC-1/CCL14 HB-EGF Haptoglobin FABP1 F MILLIPLEX H2A.X (Ser139) H2A.X H Galectin-1 (GAL1) (autoantibody) Galectin-1 G FABP4 FABP3 Analyte ...... C,E,F,H,M,NHP,Por,R ...... C,H,M,NHP,R C,H,M,NHP,R C,H,M,NHP,R C,H,M,NHP,R C,H,M,NHP,R C,H,M,NHP,R E,H,M,NHP,R ® E,H,M,NHP E,H,NHP,R Analyte SpeciesIndex(continued) Analyte ..... Species H,M,NHP H,M,NHP ...... H,NHP H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R F,H,M H,M H,M H,M H,M H,R H,R H,R E,H F,H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H R R

IL-7 IL-8/CXCL8 IL-6Rα (soluble) IL-6R (soluble) IL-6 IL-5 IL-4 IL-3 IL-1Ra IL-8/CXCL8 IL-4R (soluble) IL-6 (autoantibody) IL-2 IL-1α (autoantibody) IL-1α (Ser177/181) IKKα/β (Ser32)IκBα ...... IL-2Rα (soluble) IL-1RII (soluble) IL-1RI (soluble) IL-1β IgM IgG4 IgG3 IgG2b IgG2a IGFBP6 IGFBP5 IGFBP4 IGF2BP3/IMP3 (autoantibody) IGFBP3 IGF2BP2/IMP2 (autoantibody) IGFBP2 IgG2 IgG1 IGFBP7 IGFBP1 IGF1R (Tyr1135/1136) IGF1R (panTyr) IGF1R (total) IGF1R IFNβ1 IGF-2 IGF-1 IgE IgA IFNγ HVEM (HSA) Albumin Serum Human HSP70 HSP60 (autoantibody) IFNβ (autoantibody) IFNβ IFNα2 IFNγ (autoantibody) IDO1 HSP27 (Ser78) HSP27 HMGB1 HIF-1α (autoantibody) HGFR/c-Met (soluble) HGF HER2/ErbB2 (autoantibody) Hepsin ICOS ICAM-1 (soluble) I-309/CCL1 I HE4 Hck (Tyr411) Hck Analyte ...... (autoantibody) ...... Mul/Multi-Species, NHP/Non-Human Primate, O/Ovine, Por/Porcine, R/Rat B/Bovine, C/Canine, E/Equine, F/Feline, G/Chicken, H/Human, M/Mouse, ...... B,C,E,F,G,H,M,NHP,O,Por,R B,C,E,F,G,H,M,NHP,O,Por,R ...... C,E,F,G,H,M,NHP,Por,R B,E,F,H,M,NHP,O,Por,R B,E,F,H,M,NHP,O,Por,R ...... B,E,H,M,NHP,O,Por,R ...... B,C,E,F,H,NHP,O,Por ...... E,H,M,NHP,R ...... C,H,M,NHP H,NHP,Por Species G,H,NHP H,M,R H,M,R H,M H,M H,M H,M H,M H,M H,M H,M H,M H,M H,M H,M H,M H,M M M M H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H I-TAC/CXCL11 (total) IRS1 (Ser636) IRS1 Irisin (Tyr1162/1163) IR (panTyr) IR (total) IR IP-10/CXCL10 Involucrin IL-36Ra/IL-1F5 IL-36β/IL-1F8 IL-35 IL-33/NF-HEV (mature) IL-32α IL-31 Insulin IL-38/IL-1F10 IL-37/IL-1F7 IL-34 IL-29/IFNλ1 IL-28B/IFNλ3 IL-28A/IFNλ2 IL-27 IL-23 IL-22 (autoantibody) IL-22 IL-21 IL-20 IL-24 IL-19 IL-18BPa IL-18 IL-17E/IL-25 IL-17F (autoantibody) IL-17F L1A/F IL-17A IL-17A/CTLA8 (autoantibody) L1A/ LA8 TL /C IL-17A IL-16 IL-15 (autoantibody) IL-15 IL-14/α -Taxilin...... IL-13 (p40) IL-12/23 (p70) IL-12 IL-12...... (autoantibody) (p40) (p40) IL-12 IL-12 IL-11 IL-10 (autoantibody) IL-10 Jo-1 (autoantibody) (total)JNK/SAPK1 IL-9 ...... Ku (autoantibody) KIM-1 Keratin-6 Keratin-1, 10 ...... KC/GRO/CINC-1/CXCL1 KC-like Protein Kallikrein-6 K JNK/SAPK1 (Thr183/Tyr185) JNK/SAPK1 Jag1 J Analyte ...... B,C,E,G,H,M,NHP,O,Por,R ...... B,C,E,H,M,NHP,O,R ...... C,E,F,H,NHP,Por,R B,E,H,M,NHP,O,R E,F,H,M,HNP,R . C,H,M,NHP,R C,H,M,NHP,R E,H,M,NHP,R ...... G,H,M,NHP Species H,M,NHP H,M,NHP H,M,NHP H,M,NHP H,M,NHP G,H,NHP C,H,M,R H,NHP H,NHP H,M,R H,M,R H,M,R H,M,R H,M,R F,H,M NHP H,M H,M H,M H,M H,M H,M H,M B,O H,R F,M Por M H H H H H H H H H H H H H H H H H H H H H H H H H C

MILLIPLEX® ANALYTE INDEX FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

MILLIPLEX® PANELS

H H H H H H H H H H H H H H H H H H H H H H H H H H H H H M F,H H,R H,R C,H H,M H,M H,M H,M H,M H,M H,M ...... F,H,M H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R Species ...... C,H,M,NHP,R ...... E,F,G,H,M,HNP,R ...... SigmaAldrich.com/milliplex | 19 SigmaAldrich.com/milliplex ...... Analyte Proteinase 3 (autoantibody) 3 Proteinase (free) PSA PSA (total) . P-Selectin (soluble) PTEN (total) PTEN ...... (Ser380) PTH ...... PVR/CD155 PYY (total) . ProMMP-9 S S100B SARS-CoV-2 N R RAGE (soluble) RANKL ...... RANTES/CCL5 Ras (total) RasG12D (total). RasG12V (total) RBP4 ...... Renin Resistin ...... Ribosomal (autoantibody) P (Ribonucleoprotein) RNP (autoantibody) RNP/Smith (RNP/Sm) (autoantibody) (RNP/Sm) RNP/Smith RPS6 (Ser235/Ser236) RPS6 (total) SARS-CoV-2 RBD SARS-CoV-2 Spike...... S1 SARS-CoV-2 Spike S2...... SCF (autoantibody) Scl-70 Sclerostin (SOST) ...... SDF-1/CXCL12 SDH Secretin Serum Amyloid A (SAA) ...... Serum Amyloid P (SAP) Sex Hormone Binding Globulin (SHBG) ...... Siglec-7 Siglec-9 (autoantibody) Sm SMAD2 (Ser465/467) SMAD2 SMAD3 (Ser423/425) SMAD4 (total) SOX2 (autoantibody) SOX2 Src (Tyr419) Syndrome-related (Sjögren’s SSA/Ro52 (autoantibody) antigen kDa) A/Ro52 SSA/Ro60 (Sjögren’s Syndrome-related Syndrome-related (Sjögren’s SSA/Ro60 (autoantibody) antigen A/Ro60 kDa) Syndrome-related (Sjögren’s SSB/La (autoantibody) antigen B/La) S TAT1 (Tyr701) ...... (Tyr690) STAT2 STAT3 (total) S TAT3 (Ser727) S TAT3 (Tyr705) (total) STAT5A/B STAT5A/B ...... (Tyr694/699) ...... (Tyr641) STAT6 Substance P ...... Superoxide Dismutase 1 (soluble) (sSOD1)

F H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H M M Mul M,R H,M H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,NHP C,H,M,R C,H,M,R C,H,M,R Species ...... C,H,M,NHP,R ...... Analyte NCAM (soluble) O Oncostatin-M ...... (OSM) Osteoactivin Osteocalcin ...... (OC) Osteocrin (OSTN)/Musclin Osteonectin/SPARC Osteopontin ...... (OPN) Osteoprotegerin (OPG) OX40/CD134 Oxytocin P (autoantibody) P16-INK4a p21 (total) p38/SAPK2A/B (Thr180/Tyr182) p38/SAPK2A/B (total) (autoantibody) p53 N NAP-2/CXCL7 Neurogranin (NRGN) (NSE) Neuron-specific Enolase (soluble) Neuropilin-1 NGF ...... Notch1 p53 (Ser15) p53 (Ser46) ...... Kinasep70S6 (total)...... Kinase (Thr389/412) p70S6 (total) ...... PAI-1 Pancreatic Polypeptide (PP) ...... PARK5/UCHL1 (Parkinson’sPARK7/DJ1 Disease Protein 7) ...... PCNA (Proliferating Cell Nuclear (autoantibody) Antigen A) PD-1 PDGF-AA PDGF-AB/BB PDGF-BB ...... PDGFRα (panTyr) NCAML1/L1CAM/CD171 Nectin-2 Nectin-4 PDGFRβ ...... (panTyr) PD-L1 ...... PD-L2 PEDF ...... (PTX3) Pentraxin-3 Perforin Periostin ...... Progranulin (pGRN) (Alanyl-tRNA Synthetase)PL-12 (autoantibody) Placental Growth Factor 2...... Placental Growth Factor (PLGF) ...... Platelet Factor 4 (PF4)/CXCL4 ...... (autoantibody) 100 PM/Scl Progesterone Prolactin Neurotensin Neutrophil Elastase-2 (ELA2) . (total) NFκB NFκB (Ser536) NGAL/Lipocalin-2 NOXA/Mcl-1 (interaction) NT-proBNP

R H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H M .. H,R H,R M,R H,M H,M H,M H,M H,M H,M H,M H,M H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R H,M,R G,H,M H,M,NHP .... Species Analyte (continued) Index Species ...... G,H,M,NHP ...... ® C,H,M,NHP,R C,H,M,NHP,R B,H,M,NHP,O,R B,G,H,M,NHP,O ...... B,C,E,F,H,M,NHP,R ...... Analyte B/Bovine, C/Canine, E/Equine, F/Feline, G/Chicken, H/Human, M/Mouse, H/Human, M/Mouse, G/Chicken, F/Feline, E/Equine, C/Canine, B/Bovine, R/Rat Por/Porcine, O/Ovine, Primate, NHP/Non-Human Mul/Multi-Species, M Mannose-Binding Lectin (MBL) MCAM/MUC18/sCD146 (interaction) Mcl-1/BIM (total) Mcl-1 L Lactotransferrin (LTF) MILLIPLEX LAG-3 L AT (panTyr) Lck (Tyr394) Leptin . Leucine-rich 1 alpha-2-glycoprotein (LRG1) Lck (panTyr) Leukemia Inhibitory Factor (LIF) LH LIGHT LIX/CXCL6/GCP-2 L-Selectin (soluble) L-Selectin Lymphotactin/XCL1 Lyn (Tyr397) MCP-1/CCL2 MCP-3/CCL7 MCP-4/CCL13 MCP-5/CCL12 MCP-2/CCL8 M-CSF ...... M-CSFR (panTyr) MDC/CCL22 MDH1 MDM2 (total) MDM2 Melatonin Mesothelin (autoantibody) Mi-2 MEK1 (Ser222) Melanoma Inhibitory Activity Protein (MIA) MICA MICB Midkine MIP-1β/CCL4 MIP-1δ/MIP-5/CCL15 MIF MIG/CXCL9 MIP-1α/CCL3 MIP-3β/CCL19 MIP-2/CXCL2 MIP-3α/CCL20 MIP-4/PARC/CCL18 ...... MMP-1 MMP-2 MMP-3 MMP-7 Myeloperoxidase . (MPO) (autoantibody) (MPO) Myeloperoxidase Myoglobin mTOR (total) mTOR (autoantibody) (MUC1) Mucin-1 MMP-8 MMP-9 MMP-10 MMP-12 MMP-13 MPIF/CCL23 MSK1 (Ser212) mTOR (Ser2448) Myosin Light Chain 3 (MYL3) ...... Myostatin/GDF8 ...... FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® ANALYTE INDEX

MILLIPLEX® PANELS 20 |SigmaAldrich.com/milliplex TIMP-2 TIMP-1 TIM-3 Tie-2 (soluble) (panTyr) TIE2 (panTyr) TIE1 Thrombospondin-2 (TSP-2) Thrombospondin-1 (TSP-1) Thrombomodulin TGFβRII (total) ...... TGFβ3 TGFβ2 TGFβ1 ...... TGFα TFF-3 Testosterone Tenascin-C (TN-C) Tau (Thr181) ...... Tau (Total) TARC/CCL17 T4 (panTyr)Syk ...... Survivin/BIRC5 (autoantibody) T3 T (sSOD2) 2(soluble) Dismutase Superoxide MILLIPLEX R/Rat pp. 29-30;Allotherspeciesp. pp. 31;CellSignalingkits 32-35 H/Human pp. 22-26;M/Mousepp. 27-29;NHP/Non-Human Primatep. 27; Mul/Multi-Species, NHP/Non-Human Primate, O/Ovine, Por/Porcine, R/Rat B/Bovine, C/Canine, E/Equine, F/Feline, G/Chicken, H/Human,M/Mouse, Analyte ...... What’s intheMILLIPLEX ...... ® ...... Analyte SpeciesIndex(continued) Analyte Species H,NHP H,M,R Mul,R Mul,R H,M H,M C,H Mul Mul Mul Mul H H H H H H H H H H H H H H ...... TWEAK ...... TSLP ...... TSH (Ser939) ...... TSC2 (total) TSC2 Troponin T (TnT) T Troponin Troponin I (TnI) I Troponin TREM2 ...... TRAP5 Transglutaminase 2 (TGM2) TRAIL/TNFSF10 TPO (soluble) (sTNFR2) RII . TNF (soluble) (sTNFR1) RI TNF ...... (LTA)TNFβ/Lymphotoxin-α (total)TNFR1 TLR-2 ...... (TF) Factor Tissue ...... TIMP-4 TIMP-3 TNFα TNFα Uteroglobin Uromodulin uPAR (soluble)/CD87 . ucMGP U Analyte ® kit? ...... (autoantibody) ......

...... B,C,E,F,H,M,NHP,O,Por,R ...... C,H,M,NHP,R Species H,M,NHP H,M,R H,M,R H,M H,M H,M H,M H H H H H H H H H H H H H H H H H ...... Protein DBinding Vitamin VEGFR3/Flt-4 (soluble) VEGFR3/Flt-4 (panTyr) VEGFR2/KDR/Flk-1 (soluble) VEGFR2/KDR/Flk-1 (panTyr) VEGFR1/Flt-1 (soluble) VEGFR1/Flt-1 (panTyr) VEGF-D ZAP-70 (panTyr) ...... Z YKL40/CHI3L1 Yes (Tyr421) ...... Y VEGF-C VEGF-A VEGF VCAM-1 (soluble) V Analyte ...... B,H,M,NHP,O,R ...... Species H,M,R H,M,R H,M H,M H,M H,M H,M G,R H H H H H H MILLIPLEX® PREMIXED KIT INDEX FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

MILLIPLEX® PANELS p. 31 p. p. 31 p. p. 31 p. p. 31 p. p. 31 p. p. 31 p. p. 29 p. SigmaAldrich.com/milliplex | 21 SigmaAldrich.com/milliplex p. 28 p. 28 p. p. 24 p. Cat. No. HGAMMAG-301K Cat. No. Mouse Mouse Soluble Cytokine Panel, Receptor MSCRMAG-42K Cat. No. Mouse Immunoglobulin Isotyping Panel, MGAMMAG-300K Cat. No. Equine Equine Cytokine Panel, EQCTMG93KPX23BK EQCYTMG-93KPX23, 23-Plex Cat. Nos. Feline 31 Cytokine p. Feline Panel, FCYTMAG20KPX19BK FCYTMAG-20K-PMX, 19-Plex Cat. Nos. Ovine Ovine Cytokine 1, Panel SCYT-91K-PXBK14 SCYT-91K-PX14, 14-Plex Cat. Nos. Porcine Cytokine Porcine Panel, PCYTMAG23PMX13BK PCYTMG-23K-13PX, 13-Plex Cat. Nos. Multiple Species 23, 27, 28, 31 pp. TGFβ, Multi-Species TGFBMAG-64K-03 3-Plex Cat. No. Only Custom Premix Human Human Immunoglobulin Isotyping Panel, Rat Rat Cytokine Panel, RECYMAG65PMX27BK RECYMAG65K27PMX, 27-Plex Cat. Nos. Bovine Cytokine 1, Bovine Panel BCYT1-33K-PXBK15 BCYT1-33K-PX15, 15-Plex Cat Nos. Canine Canine Cytokine Panel, CCYTMAG90KPX13BK CCYTMG-90K-PX13, 13-Plex Cat. Nos. Chicken Cytokine Chicken Panel, GCYT1-16K-PXBK12 GCYT1-16K-PX12, 12-Plex Cat. Nos. p. 27 p. p. 27 p. p. 27 p. , we offer a custom premix service where you can select your select you can service where a custom premix , we offer p. 22 p. p. 27 p. p. 22 p. p. 22 p. p. 22 p. p. 22 p. p. 27 p. 27 p. Premixed and Bulk Premixed Kits Premixed Bulk and Premixed p. 23 p. ® p. 28 p. p. 28 p. Mouse CD8+ T Cell, MCD8MAG48KPX15BK MCD8MAG48K-PX15, 16-Plex Cat. Nos. 18-Plex Cat Nos. MHSTCMAG-70KPMX, MHSTCMAG-70KPXBK MHSTCMAG-70KPMX, 18-Plex Cat Nos. Mouse Th17, MT17MAG47PMX25BK MT17MAG47K-PX25, 25-Plex Cat. Nos. Mouse Cytokine 2, Panel MECY2MAG73KPXBK MECY2MAG-73KPX, 25-Plex Cat Nos. Mouse High Sensitivity T Cell, Mouse Mouse Cytokine 1, Panel MCYTMAG70PMX25BK; MCYTOMAG-70K-PMX, 25-Plex Cat Nos. MCYTMAG70PMX32BK MCYTMAG-70K-PX32, 32-Plex Cat Nos. Non-Human Primate Cytokine 2, Panel PRCY2MG40PMX24BK; PRCYTMAG40K-PX24, 24-Plex Cat Nos. PRCY2MG40PMX25BK PRCYTMAG40K-PX25, 25-Plex Cat Nos. Non-Human Primate Cytokine 1, Panel PRCYMAG40PMX23BK PCYTMG-40K-PX23, 23-Plex Cat Nos. Non-Human Primate Non-Human Primate Cytokine A, Panel PRCYTA-40K-BK38 PRCYTA-40K-PX38, 38-Plex Cat. Nos. PRCYTA-40K-BK48 PRCYTA-40K-PX48, 48-Plex Cat. Nos. Human Immuno-Oncology Checkpoint Protein Human Immuno-Oncology Checkpoint 25 1, p. Panel HCKP1-11K-PXBK17 HCKP1-11K-PX17, 17-Plex Cat. Nos. Human Diabetes, p. 24 p. Human Diabetes, HDIAB34KPMX5BK HDIAB-34K-PMX5, 5-Plex Cat. Nos. Human CD8+ T Cell, HCD8MAG15KPX17BK HCD8MAG15K17PMX, 17-Plex Cat. Nos. 23 p. Human Soluble Cytokine Receptor, HSCRMAG32PMX14BK HSCRMAG32KPX14, 14-Plex Cat. Nos. 21-Plex Cat. Nos. HSTCMAG384-PX21, HSTCMAG384PX21BK HSTCMAG384-PX21, 21-Plex Cat. Nos. 23 Human Th17, p. HT17MAG14PMX25BK HT17MG-14K-PX25, 25-Plex Cat. Nos. 13-Plex Cat. Nos. HSTCMAG28SPMX13, HSTCMAG28PMX13BK; HSTCMAG28PMX13BK; HSTCMAG28SPMX13, 13-Plex Cat. Nos. HSTCMAG28PMX21BK HSTCMAG28SPMX21, 21-Plex Cat Nos. 384-well Human High Sensitivity 22 T Cell, p. Human Cytokine Panel IV, Human Cytokine IV, Panel HCP4MG64KPX20BK HCY4MG-64K-PX20, 20-Plex Cat. Nos. Human High Sensitivity T Cell, Human Cytokine II, Panel HCP2MAG62KPX23BK HCP2MAG-62K-PX23, 23-Plex Cat. Nos. Human Cytokine I, Panel HCYTMAG60PMX29BK; HCYTMAG-60K-PX29, 29-Plex Cat Nos. HCYTMAG60PMX30BK; HCYTMAG-60K-PX30, 30-Plex Cat Nos. HCYTMAG60PMX38BK; HCYTMAG-60K-PX38, 38-Plex Cat Nos. HCYTMAG60PMX41BK HCYTMAG-60K-PX41, 41-Plex Cat Nos. Human Human Cytokine A, Panel HCYTA-60K-PXBK38; HCYTA-60K-PX38, 38-Plex Cat. Nos. HCYTA-60K-PXBK48 HCYTA-60K-PX48, 48-Plex Cat. Nos. We strive to provide kits with the greatest flexibility and quickest possible turn-around-times, turn-around-times, possible flexibility kits and quickest with the greatest to provide strive We bead kits and premixed offer fixed we this, achieve To while maintaining our high-quality assays. For of kits. for a wide variety packaging kits in space-saver that come bulk premixed corresponding select kitssymbol noted with the MILLIPLEX analytes and our scientists will mix the beads for your convenience. Explore our list of premixed and premixed our list of Explore convenience. analytes and our scientists for your will mix the beads kits below. bulk premixed FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® IMMUNOLOGY

HUMAN 22 |SigmaAldrich.com/milliplex Growth FactorGrowth Panel A Human Cytokine/Chemokine/ Immunology HUMAN 48 48 38 38 IL-12 (p70) IL-12 (p40) IL-10 IL-9 IL-8/CXCL8 IL-7 IL-6 IL-5 IL-4 IL-3 IL-2 IL-1RA IL-1β IL-1α IFNγ IFNα2 GROα GM-CSF G-CSF Fractalkine/CX3CL1 Flt3 Ligand FGF-2/FGF-basic Eotaxin/CCL11 EGF sCD40L

(Cat. No. (Cat. HCYTA-60K-PXBK48) No. (Cat. HCYTA-60K-PX48) No. (Cat. HCYTA-60K-PXBK38) No. (Cat. HCYTA-60K-PX38) (Cat. No.(Cat. HCYTA-60K) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ TNFβ/Lymphotoxin-α TNFα TGFα RANTES/CCL5 PDGF-AB/BB PDGF-AA MIP-1β/CCL4 MIP-1α/CCL3 MIG/CXCL9 MDC/CCL22 M-CSF MCP-3/CCL7 MCP-1/CCL2 IP-10/CXCL10 IL-27 IL-22 IL-18 IL-17F VEGF-A IL-17E/IL-25 IL-17A/CTLA8 IL-15 IL-13 (LTA) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦

♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦▲ Panel I Human Cytokine/Chemokine (Cat. No.(Cat. HCYP3MAG-63K) Panel III Human Cytokine/Chemokine Panel II Human Cytokine/Chemokine 41 41 38 38 30 30 29 29 23 23 IL-8 /CXCL8 ♦ IL-7 IL-6 IL-5 IL-4 IL-3 IL-2 IL-1Ra IL-1β IL-1α IFNγ IFNα2 GRO GM-CSF G-CSF Fractalkine /CX3CL1 Flt3 Ligand FGF-2 /FGF-basic /CCL11♦ Eotaxin EGF sCD40L Lymphotactin /XCL1 LIX /CXCL6 /GCP-2 I-TAC /CXCL11 IL-29 /IFNλ1 IL-11 HCC-1 /CCL14▲ IL-23 IL-21 IL-20 IL-16 I-309/CCL1 Eotaxin-3/CCL26 Eotaxin-2/CCL24/ ENA-78/CXCL5 CTACK/CCL27 BCA-1/CXCL13 6Ckine/CCL21/

MPIF-2 Exodus-2 (Bulk Cat. No. (BulkCat. HCYTMAG60PMX41BK) No. (Cat. HCYTMAG-60K-PX41) No. (BulkCat. HCYTMAG60PMX38BK) No. (Cat. HCYTMAG-60K-PX38) No.(Bulk Cat. HCYTMAG60PMX30BK) No. (Cat. HCYTMAG-60K-PX30)

(Bulk Cat. No.(Bulk Cat. HCYTMAG60PMX29BK) No.(Cat. HCYTMAG-60K-PX29) (Bulk Cat. No.(Bulk Cat. HCP2MAG62KPX23BK) No.(Cat. HCP2MAG-62K-PX23) (Cat. No.(Cat. HCYP2MAG-62K) (Cat. No.(Cat. HCYTOMAG-60K) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ VEGF-A TNFβ /Lymphotoxin-α TNFα TGFα RANTES /CCL5♦ PDGF-AB /BB PDGF-AA MIP-1β /CCL4♦ MIP-1α /CCL3♦ MDC /CCL22 MCP-3 /CCL7 MCP-1 /CCL2♦ IP-10 /CXCL10 ♦ IL-17A ♦ /CTLA8 IL-15 IL-13 IL-12 (p70) IL-12 (p40) IL-10 IL-9 NAP-2 /CXCL7 ▲ MIP-3β /CCL19 MIP-3α /CCL20 MIG /CXCL9 M-CSF TSLP TRAIL/TNFSF10 TPO TARC/CCL17 SDF-1/CXCL12 SCF MIP-1δ/MIP-5/CCL15 MCP-4/CCL13 MCP-2/CCL8 LIF IL-33/NF-HEV IL-28A/IFNλ2 (LTA) (mature) ♦ ♦ ♦ ♦ ♦ ♦ ▲ ♦ ♦ ♦ ▲ ▲ ♦ T Cell Human HighSensitivity No.(Cat. HIL18MAG-66K) Human IL-18 Singleplex Panel IV Human Cytokine/Chemokine (Cat. No.(Cat. HSTC384-28K) TCell Sensitivity 384-well HumanHigh 21 21 20 20 21 21 13 13 IL-18 IL-24 IL-19 IL-14 /α-Taxilin IFNβ HMGB1 ● HCC-4 /CCL16 CXCL16 CCL28 BRAK /CXCL14 BAFF /Blys IL-10 IL-8 /CXCL8 IL-7 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF Fractalkine /CX3CL1 IL-10 IL-8 /CXCL8 ♦ IL-7 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF Fractalkine /CX3CL1

(Cat. No. (Cat. HSTCMAG28SPMX21) No. (BulkCat. HSTCMAG28PMX21BK) (Bulk Cat. No.(Bulk Cat. HSTCMAG28PMX13BK) No.(Cat. HSTCMAG28SPMX13) No.(Bulk Cat. HCP4MG64KPX20BK) No.(Cat. HCY4MG-64K-PX20) (Bulk Cat. No.(Bulk Cat. HSTCMAG384PX21BK) No.(Cat. HSTCMAG384-PX21) (Cat. No.(Cat. HSTCMAG-28SK) No.(Cat. HCYP4MAG-64K) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ YKL40/CHI3L1 MPIF /CCL23 MIP-4 /PARC /CCL18 IL-38 /IL-1F10 IL-37 /IL-1F7 IL-36β /IL-1F8 IL-35 IL-34 IL-32α IL-28B /IFNλ3 TNFα MIP-3α /CCL20 MIP-1β /CCL4 MIP-1α /CCL3 I-TAC /CXCL11 IL-23 IL-21 IL-17A /CTLA8 IL-13 IL-12 (p70) TNFα MIP-3α /CCL20 MIP-1β /CCL4 MIP-1α /CCL3 I-TAC /CXCL11 IL-23 IL-21 IL-17A /CTLA8 IL-13 IL-12 (p70) ♦ ♦ ♦ ♦ ♦ MILLIPLEX Human Th17 Human Complement Panel 2 Immune Response (Cat. No. HTH17MAG-14K) (Cat. No. HCMP2MAG-19K) Human Autoimmune 25 (Cat. No. HT17MG-14K-PX25) C1q C4 25 (Bulk Cat. No. HT17MAG14PMX25BK) Autoantibody** ® C3 Factor B IMMUNOLOGY / IMMUNE RESPONSE (Cat. No. HAIAB-10K) GM-CSF IL-17E / IL-25 C3b Factor H IFNγ IL-17F β2-Glycoprotein Ribosomal P IL-1β IL-21 C1q RNP Human MMP Panel 1 IL-2 IL-22 CENP-A (Centromere (Ribonucleoprotein) IL-4 IL-23 (Cat. No. HMMP1MAG-55K) Protein A) RNP/Smith (RNP/ IL-5 IL-27 CENP-B (Centromere Sm) MMP-3 MMP-13 IL-6 IL-28A / IFNλ2 Protein B) Scl-70 MMP-12 IL-9 IL-31 Jo-1 Sm IL-10 IL-33 / NF-HEV Ku SSA/Ro52 (Sjögren’s Syndrome-related IL-12 (p70) (mature) Human MMP Panel 2 Mi-2 antigen A/Ro52 MIP-3α / CCL20 Myeloperoxidase IL-13 (Cat. No. HMMP2MAG-55K) kDa) HUMAN TNFα (MPO) IL-15 SSA/Ro60 TNFβ/Lymphotoxin-α MMP-1 MMP-9 PCNA (Proliferating IL-17A / CTLA8 Cell Nuclear (Sjögren’s (LTA) MMP-2 MMP-10 Antigen A) Syndrome-related MMP-7 antigen A/Ro60 PL-12 (Alanyl-tRNA kDa) Human CD8+ T Cell Synthetase) SSB/La (Sjögren’s PM/Scl 100 (Cat. No. HCD8MAG-15K) Human TIMP Panel 1 Syndrome-related Proteinase 3 17 (Cat. No. HCD8MAG15K17PMX) (Serum/Plasma samples) antigen B/La) 17 (Bulk Cat. No. HCD8MAG15KPX17BK) (Cat. No. HTMP1MAG-54K) Human Cytokine sCD137/4-1BB/ IL-4 TIMP-1 TIMP-2 Autoantibody ** TNFRSF9 IL-5 sFAS / TNFRSF6 IL-6 (Cat. No. HCYTAAB-17K) sFasL IL-10 Human TIMP Panel 2 BAFF/Blys IL-12 (p40) GM-CSF IL-13 (Cell culture samples) G-CSF IL-15 Granzyme A MIP-1α / CCL3 (Cat. No. HTMP2MAG-54K) IFNβ IL-17A/CTLA8 Granzyme B MIP-1β / CCL4 TIMP-1 TIMP-3 IFNγ IL-17F IFNγ Perforin TIMP-2 TIMP-4 IL-1α IL-18 IL-2 TNFα IL-6 IL-22 IL-8/CXCL8 TNFα Human Soluble Cytokine Multi-Species TGFβ – IL-10 Receptor Singleplex # (Cat. No. HSCRMAG-32K) (Cat. No. TGFBMAG-64K-01) Human Sepsis Panel 1 (Bulk Cat. No. TGFBMAG-64K-01BK) 14 (Cat. No. HSCRMAG32KPX14) (Cat. No. HSP1MAG-63K) 14 (Bulk Cat. No. HSCRMAG32PMX14BK) TGFβ1 sFAS / TNFRSF6 MIF sFasL PAI-1 (total) sCD30 sIL-6R ▼ sEGFR/sHER1/ErbB1 sRAGE Multi-Species TGFβ – 3 Plex # sICAM-1 sVCAM-1 sGP130 sTNF RI (Cat. No. TGFBMAG-64K-03)

sIL-1RI sTNF RII NON-CONFIGURABLE KIT Human Sepsis Panel 2 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. sIL-1RII sVEGFR1 / sFlt-1 TGFβ1 TGFβ3 (Cat. No. HSP2MAG-63K) sIL-2Rα sVEGFR2 / sKDR / sFlk-1 TGFβ2 sIL-4R sVEGFR3 / sFlt-4 Granzyme B MIP-1α / CCL3 HSP70 MIP-1β / CCL4 Human Fibrosis Panel 1 NEW IL-1α MMP-8 Human Complement Panel 1 IL-8 / CXCL8 (Cat. No. HFIB1-100K) (Cat. No. HCMP1MAG-19K) COMP Uteroglobin Adipsin/Factor D C5a Human Sepsis Panel 3 IL-18BPa C2 Factor I (Cat. No. HSP3MAG-63K) C4b Mannose-binding Lactotransferrin (LTF) Resistin C5 Lectin (MBL) Neutrophil Elastase-2 Thrombospondin-1 (ELA2) (TSP-1) NGAL/Lipocalin-2

Legend key for MILLIPLEX® kits

† Analytes which cannot be plexed together: ◗ Requires a protease inhibitor during sample ^ Competitive assay format – Active and total collection Available for custom premix – Free and total ■ Requires sample extraction ◦ Analyte requires a different sample dilution ♦ Available in Cat. No. listed ** No standard/QC; antibody levels are from others in panel ▼ Premix panel only reported as MFIs (Median Fluorescence  Analyte cannot be measured in serum Bulk indicates Space Saver Packaging Intensities) # Requires sample acidification ● ▲ These analytes cannot be plexed with other Serum/Plasma only S Analyte cannot be measured in plasma ★ analytes in this panel in serum/plasma Tissue Culture samples only U Urine only

SigmaAldrich.com/milliplex | 23 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® IMMUNE RESPONSE / VIROLOGY / METABOLISM / ENDOCRINOLOGY / CVD

HUMAN 24 |SigmaAldrich.com/milliplex Isotyping Isotyping Human Immunoglobulin No.(Cat. SKINMAG-50K) only) (Skin extracts Human Skin (Cat. No.(Cat. HADK2MAG-61K) (Serum/Plasma samples) Human AdipokinePanel 2 No.(Cat. HADK1MAG-61K) (Serum/Plasma samples) Human AdipokinePanel 1 Endocrinology Metabolism/ Diagnostic Procedures. For Research UseOnly. NotFor UseIn No.(Cat. HC19SERM1-85K) IgM SARS-CoV-2 AntigenPanel 1 No.(Cat. HC19SERG1-85K) IgG SARS-CoV-2 AntigenPanel 1 No.(Cat. HC19SERA1-85K) ** IgA SARS-CoV-2 AntigenPanel 1 Virology No.(Cat. HGAMMAG-303E) Human IgE –Singleplex Insulin IL-8/CXCL8 IL-6 IL-1β NGAL/Lipocalin-2 Adipsin/Factor D Adiponectin SARS-CoV-2 RBD SARS-CoV-2 N SARS-CoV-2 RBD SARS-CoV-2 N SARS-CoV-2 RBD SARS-CoV-2 N IgE IgG2 IgG1 IgA Human Serum ^ Cortisol

Albumin (HSA) (Cat. No.(Cat. HGAMMAG-301K) ** ** TNFα NGF MCP-1/CCL2 Leptin Resistin PAI-1 (total) SARS-CoV-2 Spike S2 SARS-CoV-2 Spike S1 SARS-CoV-2 Spike S2 SARS-CoV-2 Spike S1 SARS-CoV-2 Spike S2 SARS-CoV-2 Spike S1 IgM IgG4 IgG3 Keratin-6 Keratin-1,10 Involucrin (Cat. No.(Cat. HMH3-34K) HormoneV3 Human Metabolic (Cat. No.(Cat. HAGE1MAG-20K) Human AgingPanel 1 No.(Cat. HMYOMAG-56K) Human Myokine No.(Cat. HMHEMAG-34K) Hormone Human Metabolic Human Diabetes No.(Cat. HADCYMAG-61K) culture(Cell samples) Human Adipocyte 5 5 GnRH GDF15 GDF-11 FGF-21 CTACK/CCL27 NON-CONFIGURABLE KIT IL-6 Glucagon ◗ GLP-1 † (total) GLP-1 (active) † GIP (total) Ghrelin (active) ◗ C-Peptide Amylin † (total) Amylin (active) † Glucagon GLP-1 (active) C-Peptide IL-6 FSTL1 Fractalkine/CX3CL1 FGF-21 FABP3 (EPO) Erythropoietin BDNF Apelin Glucagon †◗ (total) GLP-1 (active) †◗ GLP-1 GIP (total) Ghrelin (active) C-Peptide †◗ (total) Amylin (active) †◗ Amylin Leptin IL-8/CXCL8 IL-6 IL-1β HGF Adiponectin HDIAB-34K-PMX5) (Cat.No. (Bulk Cat. No.(Bulk Cat. HDIAB34KPMX5BK) ◗ ◗ ◗ ◗ ◗ ◗ ◗ ◗ IL-10 IL-6 Leptin Jag1 IL-18 TNFα Secretin PYY (total) Pancreatic MCP-1/CCL2 Leptin Insulin Leptin Insulin Osteonectin/SPARC Osteocrin (OSTN)/ (OSM) Oncostatin-M Myostatin/GDF8 LIF Irisin IL-15 TNFα PYY (total) Pancreatic MCP-1/CCL2 Leptin Insulin IL-6 TNFα Resistin PAI-1 (total) NGF MCP-1/CCL2 ▼ Polypeptide (PP) Musclin Polypeptide (PP) ★ (Cat. No.(Cat. HPTP1MAG-66K) Panel 1 Human Pituitary No.(Cat. HIGFMAG-52K) Human IGF No.(Cat. HIGFBMAG-53K) Human IGFBindingProtein No.(Cat. HLPPMAG-57K) Human Liver Protein (Cat. No.(Cat. HCVD3MAG-67K) (Acute Phase) PanelHuman CVD 3 No.(Cat. HCVD2MAG-67K) PanelHuman CVD 2 No.(Cat. HCVD1MAG-67K) PanelHuman CVD 1 Cardiovascular No.(Cat. MSHMAG-21K) Multi-Species Hormone CNTF Agouti-Related ACTH IGF-1 IGFBP4 IGFBP3◦ IGFBP2 IGFBP1 FABP1 ANGPTL6 ANGPTL4 ANGPTL3 α-Fetoprotein (AFP) Fibrinogen  Fetuin A CRP Adipsin/Factor D α-2-Macroglobulin α-1-Acid Glycoprotein Myeloperoxidase sICAM-1 GDF15 D-dimer ADAMTS13 FABP4 FABP3 Endocan-1 (ESM-1) CXCL16 CXCL6/GCP-2 CK-MB BNP Progesterone Estradiol Cortisol Protein (AgRP) (AGP) (MPO) S ■ TSH LH GH FSH IGF-2 IGFBP7 IGFBP6 IGFBP5 HGF FGF-23 FGF-21 FGF-19 Serum Amyloid P Platelet Factor 4 sL-Selectin Haptoglobin Troponin I(TnI) Growth Placental (OSM) Oncostatin-M NT proBNP LIGHT T4 T3 Testosterone sVCAM-1 Serum Amyloid A sP-Selectin NGAL/Lipocalin-2 Myoglobin (SAP) (PF4)/CXCL4 Factor (PLGF) ■

^ ◗ MILLIPLEX Human CVD Panel 4 Human Immuno-Oncology Human Circulating Cancer (Cat. No. HCVD4MAG-67K) Checkpoint Protein Panel 2 Biomarker Panel 3 (Cat. No. HCKP2-11K) (Cat. No. HCCBP3MAG-58K) sCD31/sPECAM-1 Thrombomodulin ® sE-Selectin Tissue Factor (TF) 4-1BBL/TNFSF9 FGL1/Hepassocin Cathepsin D Melanoma Inhibitory / BONE CANCER CVD Follistatin (FST) Troponin T (TnT) 5’-NT/CD73 Galectin-1 Ferritin Activity (MIA) Pentraxin-3 (PTX3) APRIL Galectin-3 Fibroblast Activation Myeloperoxidase (MPO) Arginase-1 Granulysin Protein (FAP) Galectin-3 Sex Hormone Binding Human CVD Panel 6 B7-H2/ICOSL Granzyme B Globulin (SHBG) B7-H3/CD276 IDO1 IGFBP3 ◦ (Cat. No. HCVD6MAG-67K) B7-H4/VTCN1 MICA sCD14 MCAM/MUC18 B7-H5/VISTA MICB Human Circulating Cancer DPP4 Progranulin (pGRN) B7-H6 Nectin-2 Biomarker Panel 4 BAFF/BLyS Nectin-4 Endostatin ucMGP (Cat. No. HCCB4MAG-58K) LRG1 CD25/IL-2Rα OX40/CD134

CD30/TNFRSF8 Perforin ALDH1A1 Kallikrein-6 HUMAN Human Apolipoprotein CD40L PVR/CD155 Carbonic Anhydrase Mesothelin CD137/4-1BB Siglec-7 9 (CA9) Midkine (Cat. No. APOMAG-62K) CD226/DNAM-1 Siglec-9 CD44 NCAML1/L1CAM/ EpCAM CD171 Apo AI Apo CII E-Cadherin Hepsin Transglutaminase 2 Apo AII Apo CIII (TGM2) Apo B Apo E Human Cancer Autoantibody** (Cat. No. HCABMAG-13K) Human Cancer/Metastasis Biomarker Panel 1 Bone CCNB1/Cyclin B1 IGF2BP2/IMP2 (Cat. No. HCMBMAG-22K) CTAG1B/NY-ESO-1 IGF2BP3/IMP3 Human Bone ENO1 Mucin-1 (MUC1) DKK1 Osteoprotegerin (Cat. No. HBNMAG-51K) Galectin-1 (GAL1) P16-INK4a GDF15 (OPG) Galectin-3 (GAL3) p53 Neuron-specific Periostin ACTH Osteocalcin (OC) HER2/ErbB2 SOX2 Enolase (NSE) TRAP5 DKK1 Osteopontin (OPN) HIF-1α Survivin/BIRC5 Osteonectin/SPARC TWEAK FGF-23 Osteoprotegerin (OPG) HSP60 YKL40/CHI3L1 IL-1β PTH IL-6 Sclerostin (SOST) Human Angiogenesis/Growth Insulin TNFα Human Circulating Cancer Leptin Biomarker Panel 1 Factor Panel 1 (Cat. No. HCCBP1MAG-58K) (Cat. No. HAGP1MAG-12K) Human RANKL – Singleplex α-Fetoprotein (AFP) IL-8/CXCL8 Angiopoietin-2 HB-EGF (Cat. No. HRNKLMAG-51K-01) CA125 Leptin BMP-9 HGF EGF IL-8/CXCL8 RANKL CA15-3 MIF CA19-9 Osteopontin (OPN) Endoglin Leptin CEA Prolactin Endothelin-1 Placental Growth CYFRA21-1 PSA (free)† FGF-1/FGF-acidic Factor (PLGF) Cancer sFAS/TNFRSF6 PSA (total)† FGF-2/FGF-basic VEGF-A

sFasL SCF Follistatin (FST) VEGF-C FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Immuno-Oncology FGF-2/FGF-basic TGFα G-CSF VEGF-D Checkpoint Protein Panel 1 HCGβ TNFα (Cat. No. HCKP1-11K) HE4 TRAIL/TNFSF10 Human Angiogenesis Panel 2 17 (Cat. No. HCKP1-11K-PX17) HGF VEGF-A (Cat. No. HANG2MAG-12K) 17 (Bulk Cat. No. HCKP1-11K-PXBK17) IL-6 Angiostatin/Kringle sIL-6Rα BTLA HVEM sAXL sNeuropilin-1 (sNRP-1) CD27 ICOS Human Circulating Cancer sCD31/sPECAM-1 Osteopontin (OPN) CD28 LAG-3 Biomarker Panel 2 sc-Kit/sStem Cell PDGF-AB/BB CD40 PD-1 (Cat. No. HCCBP2MAG-58K) Factor Receptor Tenascin-C (TN-C) CD80/B7-1 PD-L1 (SCFR) Thrombospondin-2 CD86/B7-2 PD-L2 Antithrombin III Extracellular Matrix sE-Selectin Protein 1 (ECM1) (TSP-2) CTLA-4/CD152 TIM-3 Complement Factor sEGFR/sHER1/sErbB1 H (CFH) Vitamin D Binding sTIE-2 GITR TLR-2 Protein sHER2/sEGFR2/ suPAR sErbB2 GITRL sVEGFR1/sFlt-1 sHER3/sEGFR3/ sErbB3 sVEGFR2/sKDR/sFlk-1 sHGFR/sc-Met sVEGFR3/sFlt-4

Legend key for MILLIPLEX® kits

† Analytes which cannot be plexed together: ◗ Requires a protease inhibitor during sample ^ Competitive assay format – Active and total collection Available for custom premix – Free and total ■ Requires sample extraction ◦ Analyte requires a different sample dilution ♦ Available in Cat. No. listed ** No standard/QC; antibody levels are from others in panel ▼ Premix panel only reported as MFIs (Median Fluorescence  Analyte cannot be measured in serum Bulk indicates Space Saver Packaging Intensities) # Requires sample acidification ● ▲ These analytes cannot be plexed with other Serum/Plasma only S Analyte cannot be measured in plasma ★ analytes in this panel in serum/plasma Tissue Culture samples only U Urine only

SigmaAldrich.com/milliplex | 25 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® NEUROSCIENCE / TOXICITY

HUMAN 26 |SigmaAldrich.com/milliplex (Cat. No.(Cat. HNS1MAG-95K) samples) (CSF Human NeurosciencePanel 1 No.(Cat. HNABTMAG-68K) samples) (CSF Human Amyloid andTau Beta Neuroscience (Cat. No.(Cat. HNDG3MAG-36K) Disease Panel 3 Human Neurodegenerative No.(Cat. HNDG2MAG-36K) Disease Panel 2 Human Neurodegenerative No.(Cat. HNDG1MAG-36K) Disease Panel 1 Human Neurodegenerative No.(Cat. HNS2MAG-95K) Human NeurosciencePanel 2 sNCAM Myeloperoxidase sICAM-1 D Cathepsin BDNF CRP C4 α-1-Antitrypsin (A1AT) Apo E Apo CIII Apo AI α-2-Macroglobulin FABP3 ApoE4 Angiogenin (ANG) Neuron-specific Acidic Glial Fibrillary α-Synuclein Amyloid 1-42 beta Amyloid 1-40 beta Bulk indicatesSpaceSaver Packaging ▲  ▼ ♦ † Legend key forMILLIPLEX (MPO) Enolase (NSE) Protein (GFAP)

brochure atSigmaAldrich.com/milliplex-faq. To perfect your methods, visitourMILLIPLEX Need SomeAdviceonRunning Assays? Premix panelonly analytes inthis panelinserum/plasma analytes cannotbeplexedThese analytes withother Available No. inCat. listed –Free and total –Active andtotal whichcannotbeplexed Analytes together: ◦ ◦ sVCAM-1 RANTES/CCL5 PDGF-AB/BB PDGF-AA PAI-1 (total) Serum Amyloid P PEDF MIP-4/PARC/CCL18 Complement Factor Complement Factor TREM2 Neurogranin (NRGN) Ferritin Transglutaminase 2 PARK7/DJ1 PARK5/UCHL1 Tau (total) pTau (Thr181) (SAP) H (CFH) C3 (TGM2) ® kits

(Cat. No.(Cat. HNCSMAG-35K) Human CircadianStress No.(Cat. HNPMAG-35K) Human Neuropeptide No.(Cat. HND3MAG-39K) PanelDisorders 3 Human Neurological No.(Cat. HNDG4MAG-36K) samples) (CSF Disease Panel 4 Human Neurodegenerative (Cat. No.(Cat. HKI2MAG-99K) (Urine samples) PanelHuman Kidney Injury 2 No.(Cat. HKI1MAG-99K) (Urine samples) PanelHuman Kidney Injury 1 Toxicity ★ ● **  ■ ◗ Neurotensin β-Endorphin α-MSH Kallikrein-6 Fetuin A◦ Contactin-1 Angiotensinogen GDNF Amyloid 1-42 beta Amyloid 1-40 beta Cystatin C Clusterin Albumin α-1-Microglobulin IP-10/CXCL10 GSTα FABP1 Collagen IV Calbindin Cortisol (AGT) Tissue Culture samples only Serum/Plasma only Intensities) reported asMFIs(MedianFluorescence antibodylevelsNo standard/QC; are Requires sampleextraction collection Requires aproteaseinhibitorduringsample ® FAQs pageanddownloadourTipsTricks Substance P Substance Oxytocin Soluble Superoxide Soluble Superoxide Osteopontin (OPN) S100B sRAGE Osteopontin (OPN) NGAL/Lipocalin-2 EGF TIMP-1 TFF-3 Renin Osteoactivin KIM-1 Melatonin Dismutase 2(sSOD2) Dismutase 1(sSOD1) Dismutase ■

^ ■

^ U S #  ◦ ^

(Cat. No.(Cat. HKI6MAG-99K) (Serum/Plasma samples) PanelHuman Kidney Injury 6 No.(Cat. HKI5MAG-99K) (Serum/Plasma samples) PanelHuman Kidney Injury 5 No.(Cat. HKI4MAG-99K) (Serum/Plasma samples) PanelHuman Kidney Injury 4 No.(Cat. HKI3MAG-99K) (Urine samples) PanelHuman Kidney Injury 3 (Cat. No.(Cat. HLINJMAG-75K) Human Liver Injury

Clusterin β-2-Microglobulin NGAL/Lipocalin-2 Collagen IV α-1-Microglobulin KIM-1 IP-10/CXCL10 FABP1 EGF RBP4 β-2-Microglobulin GSTα ARG1 5'NT/CD73 Urine only Analyte cannot bemeasuredAnalyte inplasma Requires sampleacidification cannotbemeasuredAnalyte inserum inpanel from others requiresAnalyte adifferent sampledilution Available forcustompremix Competitive assay format RBP4 Cystatin C Uromodulin TIMP-1 Osteoactivin Renin PTH Osteopontin (OPN) Uromodulin SDH MDH1 MILLIPLEX Non-Human Primate PRIMATE Cytokine/Chemokine Panel 2 MOUSE Immunology (Cat. No. PRCYT2MAG40K)

Immunology ®

24 (Cat. No. PRCYTMAG40K-PX24) IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY Non-Human Primate Cytokine/ 24 (Bulk Cat. No. PRCY2MG40PMX24BK) Mouse Cytokine/Chemokine Chemokine Panel A NEW 25 (Cat. No. PRCYTMAG40K-PX25) Panel 1 25 (Bulk Cat. No. PRCY2MG40PMX25BK) (Cat. No. PRCYTA-40K) (Cat. No. MCYTOMAG-70K) 38 (Cat. No. PRCYTA-40K-PX38)♦ sCD137/4-1BB/ IL-17E/IL-25 25 (Cat. No. MCYTOMAG-70K-PMX) ♦ 38 (Cat. No. PRCYTA-40K-BK38)♦ TNFRSF9 IL-21 25 (Bulk Cat. No. MCYTMAG70PMX25BK) ♦ Eotaxin/CCL11 48 (Cat. No. PPRCYTA-40K-PX48) IL-22 32 (Cat. No. MCYTMAG-70K-PX32) 48 (Cat. No. PRCYTA-40K-BK48) sFasL IL-23 32 (Bulk Cat. No. MCYTMAG70PMX32BK) FGF-2/FGF-basic IL-28A/IFNλ2 BCA-1 IL-15 ♦ Fractalkine/CX3CL1 IL-31 Eotaxin/CCL11 IL-13 ♦ ♦ sCD137 IL-16 NHP / MOUSE Granzyme A IL-33/NF-HEV G-CSF ♦ IL-15 ♦ CD40L ♦ IL-17A ♦ Granzyme B (mature) GM-CSF ♦ IL-17A/CTLA8 ♦ Eotaxin IL-17E IL-1α IP-10/CXCL10 IFNγ ♦ IP-10/CXCL10 ♦ sFASL ♦ IL-18 ♦ IL-2 MIP-3α/CCL20 IL-1α ♦ KC/GRO/CINC-1/ FGF-2 IL-21 ♦ ♦ IL-4 Perforin IL-1β ♦ CXCL1 Fractalkine IL-22 ♦ IL-6 RANTES/CCL5 ▲ IL-2 ♦ LIF G-CSF ♦ IL-23 ♦ IL-16 TNFβ/Lymphotoxin-α IL-3 LIX GM-CSF ♦ IL-28A (LTA) ♦ IL-17A/CTLA8 IL-4 ♦ MCP-1/CCL2 Granzyme A ♦ IL-31 IL-5 ♦ M-CSF Granzyme B ♦ IL-33 ♦ ♦ MIG/CXCL9 ♦ ♦ Multi-Species TGFβ – IL-6 IFNα2 IP-10 ♦ IL-7 ♦ MIP-1α/CCL3 IFNγ ♦ I-TAC ♦ Singleplex # ♦ MIP-1β/CCL4 ♦ ♦ IL-9 IL-1α MCP-1 (Cat. No. TGFBMAG-64K-01) ♦ IL-10 ♦ MIP-2/CXCL2 IL-1β ♦ MIG ♦ (Bulk Cat. No. TGFBMAG-64K-01BK) ♦ RANTES/CCL5 ♦ IL-1RA ♦ MIP-1α ♦ IL-12 (p40) ♦ TNFα ♦ IL-2 ♦ MIP-1β ♦ TGFβ1 IL-12 (p70) VEGF-A IL-4 ♦ MIP-3α ♦ IL-5 ♦ Perforin ♦ Multi-Species TGFβ – IL-6 ♦ RANTES ♦▲ 3 Plex ▼# Mouse Cytokine/ IL-7 ♦ TGFα ♦ Chemokine Panel 2 (Cat. No. TGFBMAG-64K-03) IL-8 ♦ TNFα ♦ NON-CONFIGURABLE KIT (Cat. No. MECY2MAG-73K) IL-10 ♦ TNFβ 15 (Cat. No. MECY2MAG-73KPX) IL-12 (p70) ♦ VEGF-A ♦ TGFβ1 TGFβ3 15 (Bulk Cat. No. MECY2MAG73KPXBK) TGFβ2 Non-Human Primate Erythropoietin (EPO) IL-17A/F Cytokine/Chemokine Panel 1 Exodus-2/ IL-20 Metabolism/ CCL21/6Ckine MCP-5/CCL12 (Cat. No. PRCYTOMAG-40K) Fractalkine/CX3CL1 MDC/CCL22 23 (Cat. No. PCYTMG-40K-PX23) Endocrinology IFNβ1 MIP-3α/CCL20 IFNγ 23 (Bulk Cat. No. PRCYMAG40PMX23BK) Non-Human Primate MIP-3β/CCL19 IL-11 TARC/CCL17 sCD40L IL-12/23 (p40) Metabolic Hormone IL-16 TIMP-1 G-CSF IL-13

(Cat. No. NHPMHMAG-45K) FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. GM-CSF IL-15 IFNγ IL-17A/CTLA8 Amylin (active) ◗ Insulin Mouse High Sensitivity T Cell IL-1β IL-18 C-Peptide Leptin (Cat. No. MHSTCMAG-70K) Ghrelin (active) ◗ MCP-1/CCL2 IL-1Ra MCP-1/CCL2 18 (Cat. No. MHSTCMAG-70KPMX) IL-2 GIP (total) Pancreatic MIP-1α/CCL3 18 (Bulk Cat. No. MHSTCMAG-70KPXBK) IL-4 MIP-1β/CCL4 GLP-1 (active) ◗ Polypeptide (PP) IL-5 TGFα Glucagon ◗ PYY (total) GM-CSF IL-10 IL-6 TNFα IL-6 IFNγ IL-12 (p70) IL-8/CXCL8 VEGF-A IL-1α IL-13 IL-10 Non-Human Primate Pituitary IL-1β IL-17A/CTLA8 Panel 1 IL-2 KC/GRO/CINC-1/ IL-4 CXCL1 (Cat. No. NHPPT1MG-46K) IL-5 LIX MCP-1/CCL2 ACTH FSH IL-6 MIP-2/CXCL2 Agouti-Related GH IL-7 TNFα Protein (AgRP) LH CNTF TSH

Multi-Species Hormone ■ ^ (Cat. No. MSHMAG-21K)

Cortisol Testosterone Estradiol T3 Progesterone T4

SigmaAldrich.com/milliplex | 27 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® IMMUNOLOGY / IMMUNE RESPONSE / METABOLISM / ENDOCRINOLOGY

MOUSE 28 |SigmaAldrich.com/milliplex Mouse Th17 (Cat. No.(Cat. MMMP2MAG-79K) (Serum/Plasma samples) Mouse MMPPanel 2 No.(Cat. MMMP1MAG-79K) (Serum/Plasma samples) Mouse MMPPanel 1 Receptor Mouse SolubleCytokine Mouse CD8+TCell 15 15 25 25 proMMP-9 MMP-3 MMP-2 sIL-6R sIL-4R sIL-2Rα sIL-1RII sIL-1RI sGP130 sCD30 IL-2 IFNγ Granzyme B GM-CSF sFasL sFas/TNFRSF6 sCD137/4-1BB/ IL-17A/CTLA8 IL-15 IL-13 IL-12 (p70) IL-10 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF sCD40L

TNFRSF9

(Bulk Cat. No.(Bulk Cat. MCD8MAG48KPX15BK) No.(Cat. MCD8MAG48K-PX15) No.(Bulk Cat. MT17MAG47PMX25BK) No.(Cat. MT17MAG47K-PX25) (Cat. No.(Cat. MSCRMAG-42K) No.(Cat. MCD8MAG-48K) No.(Cat. MTH17MAG-47K) MMP-12 MMP-8 sVEGFR3/sFlt-4 sVEGFR2/sKDR/sFlk-1 sVEGFR1/sFlt-1 sTNF RII sTNF RI sRAGE TNFα RANTES/CCL5 MIP-1β/CCL4 IL-13 IL-10 IL-6 IL-5 IL-4 TNFβ/Lymphotoxin-α TNFα MIP-3α/CCL20 IL-33/NF-HEV IL-31 IL-28B/IFNλ3 IL-27 IL-23 IL-22 IL-21 IL-17F IL-17E/IL-25 (LTA) (mature) Isotyping Mouse Immunoglobulin Immune Response No.(Cat. TGFBMAG-64K-03) (Cat. No.(Cat. MADCYMAG-72K) culture(Cell samples) Mouse Adipocyte No.(Cat. MADKMAG-71K) (Serum/Plasma samples) Mouse Adipokine Endocrinology Metabolism/ No.(Cat. MGAMMAG-300E) Mouse IgE –Singleplex Plex▼# Multi-Species TGFβ –3 No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – No.(Cat. MMMP3MAG-79K) culture(Cell samples) Mouse MMPPanel 3 NON-CONFIGURABLE KIT TGFβ2 TGFβ1 MCP-1/CCL2 Leptin IL-6 Adiponectin MCP-1/CCL2 Leptin Insulin IL-6 IgE IgG2a IgG1 IgA TGFβ1 MMP-8 MMP-3 MMP-2

(Cat. No.(Cat. MGAMMAG-300K) # TGFβ3 TNFα Resistin PAI-1 (total) TNFα Resistin PAI-1 (total) IgM IgG3 IgG2b MMP-12 proMMP-9 (Cat. No.(Cat. MSHMAG-21K) (Cat. No.(Cat. MMYOMAG-74K) Mouse Myokine No.(Cat. MMHE-44K) Expanded Hormone Mouse Metabolic No.(Cat. MADPNMAG-70K-01) (Serum/Plasma samples) Singleplex Mouse Adiponectin– Multi-Species Hormone■ ^ No.(Cat. MPTMAG-49K) Mouse Pituitary No.(Cat. MAGE1MAG-25K) Mouse AgingPanel 1 Progesterone Estradiol Cortisol IL-15 IL-6 Follistatin-like FGF-21 (EPO)★ Erythropoietin BDNF Insulin IL-6 Glucagon ◗ GLP-1 †◗ (total) GLP-1 (active) †◗ GIP (total) Ghrelin (active) ◗ C-Peptide 2 Amylin (active) ◗ Adiponectin GH FSH BDNF ACTH IL-6 GnRH FGF-21 CTACK/CCL27 Protein 1(FSTL1) T4 T3 Testosterone Osteonectin/SPARC Osteocrin (OSTN)/ (OSM) Oncostatin-M Myostatin/GDF8 LIF Irisin TNFα Secretin Resistin PYY (total) Pancreatic MCP-1/CCL2 Leptin TSH Prolactin LH TNFα Notch1 Leptin Jag1 Musclin Polypeptide (PP)

MILLIPLEX Cardiovascular Neuroscience RAT Mouse CVD Panel 1 Mouse Amyloid Beta

Immunology ®

(Cat. No. MCVD1MAG-77K) (Cat. No. MABMAG-83K) / NEUROSCIENCE / BONE CANCER / TOXICITY CVD / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY

sCD31/sPECAM-1 ProMMP-9 Amyloid beta 1-40 Amyloid beta 1-42 Rat Cytokine/Chemokine sE-Selectin sP-Selectin (Cat. No. RECYTMAG-65K) sICAM-1 Thrombomodulin Mouse Neuropeptide ■ ^ 27 (Cat. No. RECYMAG65K27PMX) PAI-1 (total) 27 (Bulk Cat. No. RECYMAG65PMX27BK) (Cat. No. RMNPMAG-83K) EGF IL-10 Mouse CVD Panel 2 α-MSH Oxytocin Eotaxin / CCL11 IL-12 (p70) β-Endorphin Substance P (Cat. No. MCVD2MAG-77K) Fractalkine / CX3CL1 IL-13 Neurotensin G-CSF IL-17A/CTLA8 sCD40L Oncostatin M (OSM) GM-CSF IL-18 CXCL16 Placental Growth KC/GRO/CINC-1/ IP-10/CXCL10 Endocan-1 (ESM-1) Factor 2 Toxicity CXCL1 Leptin Follistatin (FST) Troponin I (TnI) IFNγ LIX LIGHT Troponin T (TnT) Mouse Kidney Injury Panel 1 IL-1α MCP-1/CCL2 (Cat. No. MKI1MAG-94K) IL-1β MIP-1α/CCL3 Mouse Acute Phase Panel 2 IL-2 MIP-2/CXCL2 β-2-Microglobulin U Renin (Cat. No. MAP2MAG-76K) IL-4 RANTES/CCL5 IP-10/CXCL10 TIMP-1 IL-5 TNFα α-1-Acid Glycoprotein CRP KIM-1 VEGF-A U IL-6 VEGF (AGP) Haptoglobin α-2-Macroglobulin Serum Amyloid P Mouse Kidney Injury Panel 2 Adipsin/Factor D (SAP) Multi-Species TGFβ – (Cat. No. MKI2MAG-94K) Singleplex #

Clusterin NGAL/Lipocalin-2 (Cat. No. TGFBMAG-64K-01) Bone Cystatin C Osteopontin (OPN) (Bulk Cat. No. TGFBMAG-64K-01BK) EGF Mouse Bone TGFβ1 (Cat. No. MBNMAG-41K) Multi-Species TGFβ – ACTH Leptin 3 Plex ▼ # DKK1 Osteoprotegerin (Cat. No. TGFBMAG-64K-03) (OPG) FGF-23 NON-CONFIGURABLE KIT IL-6 Sclerostin (SOST) MOUSE / RAT Insulin TNFα TGFβ1 TGFβ3 TGFβ2

Cancer Metabolism/ Mouse Angiogenesis / Growth Factor Panel 1 Endocrinology (Cat. No. MAGPMAG-24K) Rat Adipokine

(Serum/Plasma samples) FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. sALK-1 IL-6 Amphiregulin IL-17A/CTLA8 (Cat. No. RADPKMAG-80K) Angiopoietin-2 ▲ KC/GRO/CINC-1/ IL-1β MCP-1 / CCL2 Betacellulin ▲ CXCL1 IL-6 PAI-1 (total) sCD31/sPECAM-1 ▲ Leptin Insulin TNFα EGF MCP-1/CCL2 Leptin Endoglin MIP-1α/CCL3 Endothelin-1 Placental Growth Factor 2 sFasL Rat Adipocyte Prolactin FGF-2/FGF-basic (Cell culture samples) SDF-1/CXCL12 Follistatin (FST) (Cat. No. RADPCMAG-82K) TNFα G-CSF VEGF-A Adiponectin MCP-1/CCL2 HGF VEGF-C IL-1β PAI-1 (total) IL-1β VEGF-D IL-6 TNFα Leptin

Legend key for MILLIPLEX® kits

† Analytes which cannot be plexed together: ◗ Requires a protease inhibitor during sample ^ Competitive assay format – Active and total collection Available for custom premix – Free and total ■ Requires sample extraction ◦ Analyte requires a different sample dilution ♦ Available in Cat. No. listed ** No standard/QC; antibody levels are from others in panel ▼ Premix panel only reported as MFIs (Median Fluorescence  Analyte cannot be measured in serum Bulk indicates Space Saver Packaging Intensities) # Requires sample acidification ● ▲ These analytes cannot be plexed with other Serum/Plasma only S Analyte cannot be measured in plasma ★ analytes in this panel in serum/plasma Tissue Culture samples only U Urine only

SigmaAldrich.com/milliplex | 29 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® METABOLISM / ENDOCRINOLOGY / CVD / BONE / NEUROSCIENCE / TOXICITY

RAT 30 |SigmaAldrich.com/milliplex (Cat. No.(Cat. RMYOMAG-88K) Rat Myokine No.(Cat. RMHMAG-84K) Hormone Rat Metabolic (Cat. No.(Cat. RCI1MAG-87K) Panel Injury 1 Rat Cardiac Cardiovascular No.(Cat. RTHYMAG-30K) Rat Thyroid No.(Cat. MSHMAG-21K) Multi-Species Hormone■ ^ No.(Cat. RSHMAG-69K) Rat Stress Hormone No.(Cat. RPTMAG-86K) Rat Pituitary Creatine Kinase TroponinCardiac T TroponinCardiac I T4^ T3^ Progesterone Estradiol Cortisol Corticosterone^ ACTH GH FSH BDNF ACTH IL-6 Fractalkine/CX3CL1 Follistatin-like Protein FGF-21 (EPO) Erythropoietin BDNF IL-6 Glucagon GLP-1 (active) GIP (total) Ghrelin (active) C-Peptide 2 Amylin (active) Muscle (CKM) (cTnT) (cTnI) 1 (FSTL1) ◗ ◗ ◗ ◗ TIMP-1 Myosin LightChain3 Follistatin-like Protein FABP3 TSH T4 T3 Testosterone Melatonin^ TSH Prolactin LH Osteonectin/SPARC Osteocrin (OSTN)/ Myostatin/GDF8 LIF Irisin IL-15 TNFα PYY (total) Pancreatic MCP-1/CCL2 Leptin Insulin (MYL3) 1 (FSTL1) Musclin Polypeptide (PP) ■ (Cat. No.(Cat. RV3MAG-26K) (Serum/Plasma samples) Rat Vascular Panel Injury 3 No.(Cat. RV2MAG-26K) (Serum/Plasma samples) Rat Vascular Panel Injury 2 No.(Cat. RV1MAG-26K) (Serum/Plasma samples) Rat Vascular Panel Injury 1 (Cat. No.(Cat. RKTX1MAG-37K) (Urine samples) Rat Kidney Toxicity Panel 1 Toxicity No.(Cat. RMNPMAG-83K) Rat Neuropeptide Neuroscience No.(Cat. RBN1MAG-31K) (Serum/Plasma samples) Rat BonePanel 1 Bone α-2-Macroglobulin α-1-Acid Glycoprotein sE-Selectin Adiponectin KC/GRO/CINC-1/ Connective Tissue Caveolin-1 KIM-1 IP-10 /CXCL10 GSTα^ Clusterin Neurotensin β-Endorphin α-MSH Insulin FGF-23 DKK1 ACTH (A2M) (AGP) CXCL1 (CTGF) FactorGrowth Haptoglobin sICAM-1 VEGF TNFα TIMP-1 PAI-1 (total) CCL2 MCP-1 / IL-6 VEGF-A TIMP-1 Osteopontin (OPN) P Substance Oxytocin Sclerostin (SOST) PTH Osteoprotegerin (OPG) Leptin ■ ^ (Cat. No.(Cat. RLI1MAG-92K) Rat Liver Injury No.(Cat. RKTX2MAG-37K) (Urine samples) Rat Kidney Toxicity Panel 2 GOT1 ARG1 5'NT/CD73 β-2-Microglobulin Albumin^ α-1-Acid Glycoprotein (AGP) SDH GSTα NGAL /Lipocalin-2 EGF Cystatin C MILLIPLEX® IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY / TOXICITY FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

OTHER Polypeptide (PP) Polypeptide Insulin Leptin Pancreatic (total)PYY LH Prolactin TSH / CCL2 MCP-1 NGAL / Lipocalin-2 Osteopontin (OPN) RBP4 TFF-3 Testosterone T3 T4 ◗ SigmaAldrich.com/milliplex | 31 SigmaAldrich.com/milliplex Competitive assay format assay Competitive Available for custom premix Available sample dilution a different Analyte requires from others in panel in serum Analyte cannot be measured sample acidification Requires Analyte cannot be measured in plasma Analyte cannot be measured Urine only Amylin (total) ◗ Amylin (active) Ghrelin GIP (total) ◗ (active) GLP-1 Glucagon ACTH FSH GH Clusterin C Cystatin / CXCL8 IL-8 KIM-1 Albumin β-2-Microglobulin Cortisol Estradiol Progesterone

Metabolism/ Endocrinology Canine Gut Hormone CGTMAG-98K) (Cat.No. Canine Pituitary Expanded Panel CANPIT-96K) (Cat. No. ■ ^ Hormone Multi-Species Toxicity Canine Toxicity Kidney 1 Expanded Panel (Urine samples) CKT1MAG-97K) (Cat. No. Canine Toxicity Kidney 2 Panel (Urine samples) CKT2MAG-97K) (Cat. No. (Cat. No. MSHMAG-21K) (Cat. No.

^ ◦  # S U ▼ CXCL1 TGFβ3 IL-17A/CTLA8 IL-36RA/IL-1F5 IP-10/CXCL10 MIP-1α/CCL3 MIP-1β/CCL4 TNFα VEGF-A IL-6 IL-8/CXCL8 IL-10 IL-12 IL-18 TNFα IL-13 IL-18 KC/GRO/CINC-1/ / CCL2 MCP-1 PDGF-BB RANTES / CCL5 SCF / CXCL12 SDF-1 TNFα ▼# (Cat. No. PCYTMAG-23K) (Cat. No. (Cat. No. SCYT1-91K) (Cat. No. (Cat. No. PCYTMG-23K-13PX) (Cat. No. PCYTMAG23PMX13BK) (Bulk Cat. No. (Cat. No. SCYT-91K-PX14) (Cat. No. SCYT-91K-PXBK14) (Bulk Cat. No. (Cat. No. FCYTMAG-20K-PMX) (Cat.No. FCYTMAG20KPX19BK) (Bulk Cat. No. Requires a protease inhibitor during sample Requires collection sample extraction Requires are No standard/QC; antibody levels as MFIs (Median Fluorescence reported Intensities) Serum/Plasma only samples only Tissue Culture

TGFβ1 TGFβ2 TGFβ1 IFNγ IL-1α IL-1β IL-4 IL-6 IL-8/CXCL8 IL-10 GM-CSF IFNγ IL-1α IL-1β IL-1Ra IL-2 IL-4 sFAS/TNFRSF6 Flt3 Ligand GM-CSF IFNγ IL-1β IL-2 IL-4 IL-6 / CXCL8 IL-8 (p40) IL-12 NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE ◗ ■ **  ● ★ 13 13 14 14 19 19 Multi-Species TGFβ – TGFβ Multi-Species Singleplex # TGFBMAG-64K-01) (Cat. No. TGFBMAG-64K-01BK) (Bulk Cat. No. – TGFβ Multi-Species 3 Plex TGFBMAG-64K-03) (Cat. No. Porcine Cytokine/ChemokinePorcine Ovine Cytokine/Chemokine 1 Panel Feline Cytokine/ChemokineFeline

kits ® IL-6 IL-8/CXCL8 IL-10 (p70) IL-12 IL-13 IL-17A/CTLA8 IL-18 IP-10/CXCL10 MCP-1/CCL2 RANTES/CCL5 TNFα IL-21 M-CSF MIP-1β/CCL4 MIP-3α/CCL20 RANTES/CCL5 VEGF IL-15 IL-18 / CXCL10 IP-10 KC-like / CCL2 MCP-1 TNFα IL-36RA/IL-1F5 IP-10/CXCL10 MCP-1/CCL2 MIP-1α/CCL3 MIP-1β/CCL4 TNFα VEGF-A These analytes cannot be plexed with otherThese analytes cannot be plexed analytes in this panel in serum/plasma Analytes which cannot be plexed together:Analytes which cannot be plexed and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix (Cat. No. EQCYTMAG-93K) (Cat. No. (Cat. No. GCYT1-16K) (Cat. No. (Cat. No. CCYTOMAG-90K) (Cat. No. (Cat. No. BCYT1-33K) (Cat.No. (Cat. No. EQCYTMG-93KPX23) (Cat. No. EQCTMG93KPX23BK) (Cat. No. (Cat. No. GCYT1-16K-PX12) (Cat. No. GCYT1-16K-PXBK12) (Cat. No. (Bulk Cat. No. CCYTMAG90KPX13BK) (Bulk Cat. No. (Cat. No. CCYTMG-90K-PX13) (Cat. No. (Cat. No. BCYT1-33K-PX15) (Cat. No. BCYT1-33K-PXBK15) (Bulk Cat. No.

▼ ▲  Legend key for MILLIPLEX key Legend † ♦ Bulk indicates Space Saver Packaging Bulk indicates Space Saver Eotaxin/CCL11 FGF-2/FGF-basic Fractalkine/CX3CL1 G-CSF GM-CSF GRO IFNγ IL-1α IL-1β IL-2 IL-4 IL-5 IFNα IFNγ IL-2 IL-6 IL-10 IL-16 GM-CSF IFNγ IL-2 IL-6 IL-7 / CXCL8 IL-8 IL-10 IFNγ IL-1α IL-1β IL-4 IL-6 IL-8/CXCL8 IL-10 IL-17A/CTLA8 13 13 15 15 23 23 12 12 Equine Cytokine/Chemokine Chicken Cytokine/ChemokineChicken Canine Cytokine/Chemokine Immunology Cytokine/ChemokineBovine 1 Panel OTHER SPECIES OTHER FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. CELL SIGNALING MILLIPLEX® ASSAYS

CELL SIGNALING 32 |SigmaAldrich.com/milliplex 3. 2. 1. diverse needs: for example: give you Signalingmultiplexkits thegiftoftimeandsample,Our Cell We offerthree inourMILLIPLEX kitformats Uncover howyour cellscommunicate SignalingMILLIPLEX Cell (Cat. No.(Cat. 48-611MAG) AB2▼ Akt/mTOR (Phosphoprotein) Akt/PKB Analyte GSK3α GSK3β IGF1R IR IRS1 mTOR p70S6 Kinase PTEN RPS6 TSC2 acrylamide gels acrylamide 96-well platesor Number of per plateorgel Total points data or gel samples perplate Number of 12 Samples 10 Proteins (µg) protein required Amount of result Total timeto ▼ ✸ ●●

Singleplex MAPmate™ you kits canuseasloadingcontrolsinmultiplexkits proteininthesameassaySame proteinphosphoproteinandtotal (2-plex) protein(ormixed)Phosphoprotein ortotal multiplexassays (≥3-plex)

Premix panelonly Can beplexedCan withother2Plexes Cannot plexwith otherphosphoAkt Cannot 120 (>400 data points canbemeasured points perplate) 120 (>400data 12 (>40samplescanbemeasured induplicate) Total (all 10proteinsmeasured insamesample) 2.5 hours +overnight2.5 hours incubation(~18hr) NON-CONFIGURABLE KIT 1-25 µg of total proteinperwell 1-25 µgoftotal 3 (Ser473) Phosphorylated 3 (Ser21) 3 (Ser9) 3 (Tyr1135/1136) 3 (Tyr1162/1163) 3 (Ser636) 3 (Ser2448) 3 (Thr412) 3 (Ser380) 3 (Ser235/236) 3 (Ser939) MILLIPLEX –

11Plex AB2: AB1: ® Assays H, M,R H, M,R H, M,R H, M H H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R Uses Assay Buffer2 Uses Assay Buffer1 ® Cell Signaling portfolio tomeetyour CellSignalingportfolio (Cat. No.(Cat. 48-612MAG) AB2▼ Akt/mTOR (Total) TSC2 RPS6 PTEN p70S6 Kinase mTOR IRS1 IR IGF1R GSK3β GSK3α Akt/PKB Analyte ® Assays R Rat M Mouse H Human (100-500 µg total proteinfor10gels) (100-500 µgtotal 10-50 µg total proteinperlane 10-50 µgtotal Total – 3 3 3 3 3 3 3 3 3 3 3 11Plex NON-CONFIGURABLE KIT Western Blots Days/weeks Phosphorylated 12 12 H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, R H, M,R H, M,R H, M,R H, M,R CELL SIGNALING MILLIPLEX® ASSAYS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CELL SIGNALING H, M, R H H H H H H, M, R H H H H H H H H H H H H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) Phosphorylated (pan Tyr) Phosphorylated Phosphorylated 3 (Ser473) 3 (Ser133) 3 (Thr185/Tyr187) 3 (Thr183/Tyr185) 3 (Ser536) 3 (Thr180/Tyr182) 3 (Thr412) 3 (Ser727) 3 (Tyr694/699) NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT SigmaAldrich.com/milliplex | 33 SigmaAldrich.com/milliplex Total 3 3 3 3 3 3 3 3 3 Total Total Akt/PKB CREB Erk/MAPK 1/2 JNK/SAPK1 NFκB p38/SAPK2A/B p70S6 Kinase STAT3 STAT5A/B Akt/PKB Analyte CREB Analyte Erk/MAPK 1/2 JNK/SAPK1 NFκB p38/SAPK2A/B p70S6 Kinase STAT3 STAT5A/B VEGFR3/Flt-4 VEGFR2/KDR/Flk-1 VEGFR1/Flt-1 TIE2 TIE1 PDGFRβ PDGFRα M-CSFR IR IGF1R Flt3 FGFR1 ErbB4/HER4 ErbB3/HER3 ErbB2/HER2 ErbB1/EGFR c-Met/HGFR Analyte c-Kit ▼ AB2 48-680MAG) (Cat. No. — 9 Plex (Total) Multi-Pathway ▼ AB2 48-681MAG) (Cat. No. (Phosphoprotein) RTK Human Needs) (Choose Analytes your that Meet AB1 HPRTKMAG-01K) (Cat.No. — 9 Plex (Phosphoprotein) Multi-Pathway H H H H H, M, R H, M H H H H, M, R H H H H H H H H H, M, R H, M, R H H, M, R H H, M H H, M, R H, M H, M, R H, M, R H H, M, R H, M, R H, M 7 Plex – 10 Plex – Phosphorylated Phosphorylated 3 (Ser112) Phosphorylated 3 (Ser473) 3 (Ser112) 3 (Ser70) 3 (Thr183/Tyr185) 3 (Ser46) 3 (Ser15) 3 (Ser139) 3 (Thr68) 3 (Ser345) Phosphorylated 3 (Tyr701) 3 (Ser15) 3 (Thr180/Tyr182) 3 (Ser212) 3 (Ser222) 3 (Thr183/Tyr185) 3 (Ser78) 3 (Thr185/Tyr187) 3 (Ser73) Phosphorylated 3 (Thr71) NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE 3 3 3 3 3 3 3 3 3 3 3 3 3 3

Total Total Total Total Total 7 Plex – STAT1 p53 p38/SAPK2A/B MSK1 p53 MEK1 p21 JNK/SAPK1 MDM2 HSP27 H2A.X H2A.X Erk/MAPK 1/2 Chk2 c-Jun c-Jun Chk1 Analyte ATF2 Analyte Analyte BAD ATR Analyte Akt/PKB BAD Bcl-2 Caspase-8 Active (Asp384) Caspase-9 Active (Asp315) JNK/SAPK1 p53 BAX Bcl-xL/BAD (interaction) BIM Mcl-1/BIM (interaction) Analyte Bcl-2 Bcl-xL Mcl-1 NOXA/Mcl-1 (interaction) MAPK/SAPK (Phosphoprotein) AB2 ▼ 48-660MAG) (Cat. No. (Cat. No. 48-621MAG) AB1 ▼ AB1 48-621MAG) (Cat. No. Human DNA Damage/Genotoxicity Human DNA Damage/Genotoxicity Human Bcl-2 Family Apoptosis Panel 2 - 4 Plex Apoptosis Panel Human Bcl-2 Family ▼ AB1 48-683MAG) (Cat. No. Human Bcl-2 Family Apoptosis Panel 1 - 6 Plex Apoptosis Panel Human Bcl-2 Family AB1 ▼ 48-682MAG) (Cat. No. Early Apoptosis Apoptosis Early AB2 ▼ 48-669MAG) (Cat.No. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. CELL SIGNALING MILLIPLEX® ASSAYS

CELL SIGNALING 34 |SigmaAldrich.com/milliplex (Cat. No.(Cat. 48-655MAG) AB2▼ Protein Translation —6Plex No.(Cat. 48-630MAG) AB1▼ NFκB —6Plex (Cat. No.(Cat. 48-650MAG) AB2▼ (Phosphoprotein) —8Plex Src Family KinaseActive Site No.(Cat. 48-684MAG) AB2▼ Human Ras-Raf Oncoprotein —6Plex TNFR1 NFκB IKKα/β IκBα FADD RasG12V RasG12D c-Myc Analyte Yes Src Lyn Lck Hck Fyn Fgr Blk Analyte Ras MEK1 CRAF BRAF Analyte 4E-BP1 elF-4G elF-4E elF-4B elF2a Analyte Download ourAACR 2020posterformore informationaboutthisunique multiplexkit! Learn more aboutourRas-Raf OncoproteinKit Total Total Total Total 3 3 3 3 3 3 NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Ser536) 3 (Ser177/181) 3 (Ser32) 3 (Ser194) Phosphorylated 3 (Thr37/46) 3 (Ser217/221) 3 (Ser338) 3 (Ser446) Phosphorylated 3 (Ser1108) 3 (Ser209) 3 (Ser422) 3 (Ser51) Phosphorylated 3 (Tyr421) 3 (Tyr419) 3 (Tyr397) 3 (Tyr394) 3 (Tyr411) 3 (Tyr420) 3 (Tyr412) 3 (Tyr389) Phosphorylated

NEW H H, M H H H H H H H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, R H, M,R H, M H, M,R H, R H TGFβ —6Plex No.(Cat. 48-690MAG) AB2▼ T-Cell Receptor (Phosphoprotein)—7Plex No.(Cat. 48-610MAG) AB2▼ STAT (Phosphoprotein)—5Plex (Cat. No.(Cat. 48-6 Akt1 Phospho/Total No.(Cat. 48-618MAG) AB2▼✸●● Akt Phospho/Total No.(Cat. 48-614MAG) AB2▼ TGFβRII SMAD4 SMAD3 SMAD2 ZAP-70 Syk Lck LAT Erk/MAPK 1/2 CREB CD3ε Analyte STAT6 STAT5A/B STAT3 STAT2 STAT1 Analyte Erk/MAPK 1/2 Analyte Akt/PKB Akt/PKB Analyte Analyte Akt/PKB Akt1 Akt1 3 1MAG) AB2▼✸●● Total Total Total 3 3

Total Total 3 3 – – 2Plex NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Ser423/425) 3 (Ser465/467) 3 (Thr185/Tyr187) 3 (Ser473) Phosphorylated 3 (panTyr) 3 (panTyr) 3 (panTyr) 3 (panTyr) 3 (Thr185/Tyr187) 3 (Ser133) 3 (panTyr) Phosphorylated 3 (Tyr641) 3 (Tyr694/699) 3 (Tyr705) 3 (Tyr690) 3 (Tyr701) Phosphorylated 2Plex 3 (Ser473) Phosphorylated (Ser473) Phosphorylated NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT H H H, M,R H H, M,R H, M,R H H H, M,R H, M,R H H, M H H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R CELL SIGNALING MILLIPLEX Akt2 Phospho/Total – 2 Plex JNK Phospho/Total – 2 Plex (Cat. No. 48-632MAG) AB2 ▼ ✸ ●● NON-CONFIGURABLE KIT (Cat. No. 48-622MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT

Analyte Total Phosphorylated Analyte Total Phosphorylated Akt2 (Ser474) H, M, R JNK/SAPK1 3 (Thr183/Tyr185) H, M, R Akt2 3 H, M, R JNK/SAPK1 3 H, M, R

Akt3 Phospho/Total – 2 Plex mTOR Phospho/Total – 2 Plex

(Cat. No. 48-633MAG) AB2 ▼ ✸ ●● NON-CONFIGURABLE KIT (Cat. No. 48-625MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT ® ASSAYS

Analyte Total Phosphorylated Analyte Total Phosphorylated Akt3 (Ser472) H, M, R mTOR 3 (Ser2448) H, M, R

Akt3 3 H, M, R mTOR 3 H, M

CREB Phospho/Total – 2 Plex p38 Phospho/Total – 2 Plex (Cat. No. 48-628MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT (Cat. No. 48-624MAG) AB1 or AB2 ▼ ●● NON-CONFIGURABLE KIT

Analyte Total Phosphorylated Analyte Total Phosphorylated CREB 3 (Ser133) H, M, R p38/SAPK2A/B 3 (Thr180/Tyr182) H, M, R CREB 3 H, M, R p38/SAPK2A/B 3 H, M, R

Erk/MAPK 1/2 Phospho/Total – 2 Plex STAT3 Phospho/Total – 2 Plex (Cat. No. 48-619MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT (Cat. No. 48-623MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT

Analyte Total Phosphorylated Analyte Total Phosphorylated Erk/MAPK 1/2 3 (Thr185/Tyr187) H, M, R STAT3 3 (Tyr705) H, M, R Erk/MAPK 1/2 3 H, M, R STAT3 3 H, M, R

IRS1 Phospho/Total – 2 Plex (Cat. No. 48-626MAG) AB2 ▼ ●● NON-CONFIGURABLE KIT

Analyte Total Phosphorylated IRS1 3 (Ser636) H, M IRS1 3 H, M, R FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MAPmate™ Singleplex Loading Control Assays Plex loading control MAPmate™ assays into existing MILLIPLEX® Cell Signaling Panels to enhance the panel within the guidelines provided in the protocols. Our Cell Signaling Buffer and Detection Kit (Cat. No. 48-602MAG), may be purchased if additional buffers are required. Our custom immunoassay team can assist with differently configured cell signaling assays. For more information on this program, contact your sales specialist.

MAPmate™ Kits Species Homology (Buffer) Cat. No.

β-Tubulin (total) MAPmate™ H, M, R (AB1 & AB2) 46-713MAG CELL SIGNALING GAPDH (total) MAPmate™ H (AB2) 46-667MAG

●● Can be plexed with other 2 Plexes AB1: Uses Assay Buffer 1 H Human ✸ Cannot plex with other phospho Akt AB2: Uses Assay Buffer 2 M Mouse ▼ Premix panel only R Rat

SigmaAldrich.com/milliplex | 35 Custom MILLIPLEX® Assay Development Accelerate your research with Custom MILLIPLEX® and ELISA

CUSTOM assays designed for your specific biomarker needs. • Develop new analytes • Multiplex new analytes with existing analytes • Recombine existing analytes into new panels

CUSTOM IMMUNOASSAYS AND SERVICES IMMUNOASSAYS CUSTOM • Convert existing assays to 384-well format ® • Design and verify assays for your samples • Kit bridging for lot-to-lot consistency MILLIPLEX • Custom kit reagents and formatting STEP 1 Visit either link on this page and “Request Information”; fill in your contact information along with the analyte full protein Get started names, species, and samples types; submit Multiplex it your way today! your request Accelerate your research with Custom MILLIPLEX® and ELISA assays designed for your specific biomarker needs.

• Develop new analytes • Multiplex new analytes with Download our new existing analytes • Recombine existing analytes into new panels • Convert existing assays to STEP 2 384-well format custom menu of • Design and verify assays for your samples • Kit bridging for lot-to-lot consistency services brochure Receive work plan and quotation • Custom kit reagents and formatting for a complete from sales specialist listing of services at EMDMillipore.com/ STEP 3

The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the customassay U.S. and Canada. Place purchase order and participate 31059_Custom_Milliplex_Assay_Brochure_MSIG.indd 1 14/04/20 10:13 AM in project kickoff call

Custom Assay Project Workflow Custom Assay Project Workflow SPR (Special Product Release): Leverage our knowledge base of pre-verified analyte/panel Submit Project Details combinations. Select analytes from up to three

panels and we will assess the combination y feasibility. Feasibility Assessment SPRCUS (Special Product Release — Custom):

For more complex analyte and panel combinations, easibilit or for those which have not been pre-verified, we F base feasibility upon compatible sample dilution, Project Workplan Issued matrix, sample preparation requirements, and known analyte cross-reactivity. Once ordered, and prior to kit release, we test cross-reactivity of antibodies to other analytes in the same custom panel and optimize the final assay and protocol. Should a combination fail testing, we will provide you with alternate assay options. 1 Data Review Develop a new MILLIPLEX® or ELISA assay which is fit for your purpose. We follow a milestone- 2 based approach to generate an assay that Data Review ssay

quantifies your exact proteins of interest. All A MILLIPLEX® assays are optimized to run on any Milestone Luminex® instrument. You will receive updates Development 3 Manufacture Assay and data reviews during each milestone, offering you peace of mind that your project is on schedule and meeting specifications. Ship to Customer PROCEDURES. USE IN DIAGNOSTIC FOR NOT USE ONLY. RESEARCH FOR

36 | SigmaAldrich.com/customassay MILLIPLEX® CUSTOM IMMUNOASSAYS AND SERVICES FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CUSTOM Species (Sample Types) H (S/P) M, R (S/P) M, R (S/P) H (S/P) H, M (Lys)** H, M (Lys)** H (U) H (S/P) M, R (S/P) H (S/P) H, M, R (Lys)** H, M, R (Lys)** H, M, R (Lys) H, M, R (Lys)** H, M, R (Lys)** M, R (S/P) M, R (S/P) H (S/P) H, M, R (S/P) CSF, H (S/P, Sup) H (Sup) H, M (Lys)** H, M (Lys)** M, R (S/P) H (S/P) M, R (S/P) H (U) M, R (P) H (S/P) M, R (S/P) Sup, H (S/P, Lys) H (S/P) Soluble analyte reported as MFIs No standard/QC; levels Intensities) (Median Fluorescence SigmaAldrich.com/customassay | 37 SigmaAldrich.com/customassay

Notations s ** Analyte I-FABP IGF-1 IL-18 of (Suppressor IL-33R/ST2 2) Tumorigenicity JNK/SAPK1 (Thr183/Tyr185) JNK/SAPK1 (total) prostaglandin D LPGDS (Lipocalin like synthase) C Lysozyme MIP-1γ MR-proADM (Ser2448) mTOR (total) mTOR p300 p38/SAPK2A/B (Thr180/Tyr182) p38/SAPK2A/B (total) Periostin PODXL (Transthyretin) Prealbumin Procalcitonin Prostaglandin D Synthase (lipocalin-type) Semaphorin 3A (total) STAT1 (Tyr701) STAT1 C Tenascin Globulin Binding Thyroxine TRAIL Transferrin Vasopressin VE Cadherin/Cadherin 5 Vitronectin Wnt-3a Glycoprotein Zinc-α-2 Analytes ® Cerebral Spinal Fluid Cerebral Cell/Tissue Lysates Multiple Types SupernatantsCell/Tissue Culture

Urine Tears Sample Type Key Sample Type CSF Lys Mul S/P Serum/Plasma Sup T U Species (Sample Types) H (S/P) H (S/P) H (U) H, M, R (Lys)** H, M, R (Lys)** T) H (S/P, H (S/P) H (S/P) H (S/P) H (S/P) H (S/P) M (S/P) M, R (S/P) H, M (Lys)** H (S/P) M, R (S/P) H, M, R (Lys) H, M (Lys)** H, M, R (Lys) H (S/P) H (S/P) H (U) H (Lys)** H (S/P) H (S/P) H, M, R (Lys)** H, M, R (Lys)** H (U) M, R (S/P) H, M, R (S/P) M (S/P) M, R (S/P) H (S/P) M, R (S/P) Mouse Human Rat Species Key H M R α-1 Antichymotrypsin α-1 Antichymotrypsin α-2 Antiplasmin Glycoprotein) (α 1-Acid AGP Akt/PKB (Ser473) Akt/PKB (total) Angiopoetin-1 Anti-Cardiolipin antibody IgA IgG VCA Anti-EBV IgM IgG, Anti-Phosphatidylserine IgM IgG, IgA, Anti-β2 Glycoprotein Apolipoprotein A-IV Arginase 1 AXL (s) (Ser112) BAD BCMA (B-cell Maturation Antigen) BMP-9 BRD4 Caspase 3 (Active) CBP CD13 Aminopeptidase N CD163 (s) Ceruloplasmin (total) PARP Cleaved B) CSTB (Cystatin Elafin ERK/MAPK 1/2 (Thr185/Tyr187) ERK/MAPK 1/2 (total) G3BP (Galectin 3 Binding Protein) Galectin-3 Gas-6 Granzyme A Gremlin GRP78 HGFR/cMET Analyte Custom MILLIPLEX Custom FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. LUMINEX® SYSTEMS

LUMINEX® PLATFORM 38 |EMDMillipore.com/luminex provide you thepowerof Luminex available, ofmultiplexanalytes portfolio we instrument solutions. Combinedwiththelargest platform forbiomarker screening andprotein technology, themosttrusted,widelyused Luminex Enhance your generationwithourLuminex data Driving AssayConsistency Luminex Multiplexed Biomarker Analysis Using Specifications • • • results, whichinstrumentascientist usesfortheirresearch. nomatter tofitthecapabilitiesof each platform. This software adapts All systems run ontheLuminex APD =avalanche photodiodesand PMT=photomultipliertubes Description Cat. No.Cat. LIS Security Automation CFR 21Part 11 Weight (Analyzer) (W xDH) Dimensions includingPC Dynamic Range Applications Read Time(MicrotiterPlate) Multiplex Capacity Bead Compatibility Hardware Optics Instrument Software including high-throughputwashingoptions. andfasterwashingtechniques,enabling better toamagneticfield, rapidly andefficiently Magnetic bead-basedformat:Responds sample usingamaximumof25µLperwell. time, screen more inasingle than40analytes Low samplevolume: Withminimalhands-on multiple parameters. toexplore sets user toconstructcleanerdata is lesspronetousererror, enablingtheend- generatedsimultaneously Multiplexing: Data ® xMAP ® technology offers many advantagescompared tootherimmunoassay technologyoffers methods: ® Systems ® xPONENT The fastest,mostflexibleplatform. ® ® 4.3 data acquisition platformprovide 4.3data aconsistentexperience, inputto fromdata Magnetic andnonmagnetic xMAP ~75 min/384-wellplate FLEXMAP 3D ~20 min/96-wellplate Flow Cytometry-based Protein/Nucleic Acid Lasers/APDs/PMTs 110 x6263cm 77.1 kg (170lbs) xPONENT ® 4.5 logs 40-014

500 3 3 3 3 ® ® System 4.3 ®

quickly andreliably. technology enableyou togainmore information and industry-leading experiencewithmultiplex accessories, andsoftware. Ourongoingdedication preferred distributorofLuminex analysis. AsaLuminex • • comprehensive socriticaltoyour data work. do your work faster, gainingtheearlyand 96- or384-wellformat,enablingyou to of different inasinglesample, analytes in measure theconcentrationofalargenumber Speed/high-throughput: Simultaneously cannot provide. thatsolid-phasedflatarraysstandardization of xMAP Reproducibility: High-volume production ® microspheres allowsassay Gen 2oftheoriginalwidelyusedLuminex 100 (80forMagPlex Magnetic andnonmagnetic Luminex ~40 min/96-wellplate Flow Cytometry-based ® Protein/Nucleic Acid Lasers/APDs/PMTs 115 x6050cm partner, weare a 49 kg (113lbs) 100™ System. xPONENT 3.5 logs ® 40-012 200™System 3 3 3 3 ® ® ® instruments, microspheres) 4.3 ®

LUMINEX® SERVICE / CONSUMABLES / TRAINING FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

LUMINEX® PLATFORM Cat. No. Cat. No. Cat. No. Cat. No. Cat. No. 40-50030 40-50021 40-50023 SVCLUMPLT SVCLUMSLV SVCLUMBRZ SVCLUMGLD F3D-CAL-K25 MPX-CAL-K25 TRONSITE-MA LX2R-CAL-K25 F3D-PVER-K25 MPX-PVER-K25 SVCLUMPLT360 TRONSITE-MPX LX2R-PVER-K25 SVCLUMGLD360 TRONSITE-FM3D SVCLUMPLTFM3D SVCLUMSLVFM3D TRONSITE-LX200 SVCLUMBRZFM3D SVCLUMGLDFM3D VP-LX-XP0N-IQ0Q TRONSITE-BIOTEK TRONSITE-MAGPIX SVCMAGPIXPLATPM SVCLUMGLDMAGPIX SVCMAGPIXGOLDPM SVCLUMPLT360FM3D VP-FM3D-XP0N-IQ0Q SVCLUMGLD360FM3D VP-MAGPIX-XP0N-IQ0Q EMDMillipore.com/luminex | 39 EMDMillipore.com/luminex 1 L 20 L • • • • • 25 uses 25 uses 25 uses 25 uses 25 uses 25 uses 2 (PM) Pack Size Pack 4 pack, 750 mL ea • • • • • • • • • 1 (PM) technology only ® instrument. Our staff can help you install Our staffinstrument. the help can and platform, to use your train you instrument, appropriate with the to supply you continue run it as required. to reagents

Repair ) ® Unlimited Emergency

® • • • • • • • • • • • • • • • instruments, instruments, ) ® ® NEW Remote Remote Support Unlimited NEW NEW response Onsite 2-business days Calibration Kit Kit Verification Performance , Gold , Gold 360* , Platinum , Platinum 360* , Bronze , Silver IQ/OQ System Onsite Installation and Training System ® ® ® ® ® ® ® ® ® ® Analyst 5.1 Software Onsite Installation 5.1 Software and Training Analyst Kits Training ® ® System System 200™ Calibration Kit (xPONENT Kit (xPONENT Verification 200™ Performance 200™, Gold 200™, Gold 360* 200™, Platinum 200™, Platinum 360* 200™, Bronze 200™, Silver 200™ System Onsite Installation and Training 200™ System 200™ IQ/OQ Drive Fluid PLUS (RUO) (RUO) Fluid PLUS Drive Calibration Kit Kit Verification Performance , Platinum , Standard , Gold System Onsite Installation and Training System IQ/OQ ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® Washer Onsite Installation and Training Washer Sheath Fluid PLUS (RUO) (RUO) Sheath Fluid PLUS (RUO) Sheath Concentrate PLUS ® ® ® 1 Year Warranty Plans Warranty 1 Year Luminex Luminex Luminex MAGPIX FLEXMAP 3D FLEXMAP 3D FLEXMAP 3D Description Description MAGPIX Luminex MILLIPLEX MAGPIX Luminex Luminex Luminex FLEXMAP 3D BioTek FLEXMAP 3D FLEXMAP 3D MILLIPLEX Luminex FLEXMAP 3D FLEXMAP 3D MAGPIX Luminex Luminex xMAP xMAP MAGPIX FLEXMAP 3D MAGPIX MAGPIX MAGPIX Luminex FLEXMAP 3D Onsite 1-business day response response Onsite 1-business day 1 (PM) = includes 1 preventive maintenance 2 (PM) = includes 2 preventive maintenances 2 preventive maintenance 2 (PM) = includes 1 (PM) = includes 1 preventive Luminex by developed * 360 service includes on-site support for assays Training/IQ/OQ Consumables Service Contracts As a preferred partner of Luminex As a preferred Instrument and Kit Service, Consumables, and Training Service,and Kit Instrument Consumables, engineers tailored to the use of your new engineers of your tailored to the use we supply full servicewe supply of sale and at point service Multi-level research. your throughout from Luminex cover contracts provide FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. WASHING & DISPENSING FOR MILLIPLEX® ASSAYS

BIOTEK® SYSTEM 40 |EMDMillipore.com/biotek allows users todispensekitreagents,allows users ensuringexcellent assay reproducibility. magnetic separationfor96-and384-wellplatewashingsystems. TheMultiFlo™ FXdispenser In partnership withBioTekIn partnership BioTek MILLIPLEX BioTek BioTek BioTek BioTek Description BioTek ® ® ® ® 405™TSMagnetic/Vacuum Filtration96-wellWasher CompletewithTouch Screen andUltrasonic Cleaning 405™TSMagnetic96-wellWasher CompletewithTouch Screen andUltrasonicCleaning 405™LSMagnetic/Vacuum Filtration96-wellWasher 405™LSMagnetic96-wellWasher ® 405™SelectTS(touchscreen) Washer ® Washing &DispensingSolutionsfor ® Assays ® instruments, weofferthelatestadvancements infullyautomated Reagent Dispenser • • • • instrumentforMILLIPLEX The perfect BioTek beads are securely heldinthewellsby theblockmagnets. andblottheremaindercontents onapapertowel.Magnetic loss ofassay performance. Simplydecantor“flick” theliquid A low-costalternative without toautomatedplatewashers 96-­ Handheld MagneticSeparatorBlockfor BioTek Description or ConicalWell Plates Handheld MagneticSeparatorBlock for96-wellFlatBottom Description precise liquiddelivery Self-contained, programmablereagent dispenserenables cost ofalargerrobotforfractionthe The performance Optimized forboth384-and96-wellplates controls,quality andsamples, andwalkaway Fully automatedprocess, justloadyour standards, well Flat Bottom or Conical Well orConical well FlatBottom Plates ® MultiFlo™ FXAutomatedReagent Dispenser ® MultiFlo™ FXAutomated • • BioTek The BioTek Fast andhands-free fullplatewashing toughest sampletypes cleaning programforeasy washingofthe andtheUltrasonicAdvantage™ user interface well optionsincludeanintuitive touch-screen ® washeradvantages: ® 405™TSmodelsin96-and384- ® 384-wellAssays Cat. No.Cat. Cat. No.Cat. 40-099 40-285 Cat. No.Cat. 40-097 40-096 40-095 40-094 BELYSA™ IMMUNOASSAY CURVE FITTING SOFTWARE FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

SOFTWARE .csv output file .csv ® SigmaAldrich.com/belysa | 41 SigmaAldrich.com/belysa xPONENT ® assay from a Luminex assay ® instruments, SMCxPRO™ platform, platform, SMCxPRO™ instruments, ® Immunoassay Curve Fitting Software Immunoassay ™ Software Key Features and Benefits Features Software Key 7/10 compatible ® ™ User-friendly interface User-friendly cubic spline) fitting 5pl, linear, (4pl, curve Automatic and manual comparison tools Curve data flagging Rules-based Luminex Suitable for use with RUO Sample concentrations of standards Recovery Equation of line Limit of Detection (LoD) Lower Limit of Quantitation (LLoQ) Minimum Detectable Dose (MD) .txt, and .pdf Data is exportable .csv, to: Excel, and ELISA .csv acquisition files and ELISA .csv Windows Belysa™ standard curve and data analysis: IL-6 MILLIPLEX standard IL-6 and data curve analysis: 1. Belysa™ Example • • • • • Belysa (Cat. No. 40-122) (Cat.No. of data and comparison proprietaryOur newest fitting, examination, for curve package software designed to provide has been software Belysa™ platforms. of immunoassay a variety from acquired integrityand confirm the fit to optimize curve tool of data or across within a plate an easy-to-use runs. plates from multiple Belysa Once the user selects the curve fit model, either automatically by using the wizard, or manually, by using the wizard, or manually, Once the user selects fit model, either automatically the curve displayed: the data are • • • • • • • As shown in Example 1, a .csv output file is simply drag-and-dropped into the Belysa™ browser into the Belysa™ output file is simply drag-and-dropped 1, a .csv As shown in Example for instant data review. • FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. BELYSA™ IMMUNOASSAY CURVE FITTING SOFTWARE

SOFTWARE 42 |SigmaAldrich.com/belysa Comparison of two standard curves inBelysa™Example standard 3.Comparisonoftwo ofthe sameMILLIPLEX lots software fromtwo • • acrossmultipleruns: Comparison tests curve isusedasaoutput files;thefirst reference againstwhicheachsubsequentcurve iscompared. software provides frompreviously acurve comparisontoolformultiplecurves imported saved Belysa™ within specificrunsor over ofalongitudinalstudy. thecourse AsshowninExample 3,Belysa™ curves provideStandard crucialinformationonthereproducibility ofanassay over multipleplates Belysa Example 2.Belysa™ belowLLoQ(yellow (pinkflags) annotation: triangle),%CV data Autoflagging Data Relative potencyofstandards Parallelism ™ Software Curve Comparison Tool Comparison Curve Software then berecorded ifrequired forreview. be madetoexclude whichwill points criteria.Then,adecisioncan acceptance thatdoesnotmeettheuser’sdata creates totheraw apinkflagnext which orbeadcounts %CV acceptable user may suchas defineparameters (BLOQ) usingayellow triangle. The such asbelowthelimitofquantitation forhardrules data software annotates As showninExample 2,Belysa™ ® assay run1year apart. Look Closer All biomarker kits are not created equal

With assay performance data included in every protocol, you can discover with confidence.

Rigorous Assay Development Reproducible Results You Can Trust Specificity Stability • Antibody specificity is tested to ensure negligible cross-reactivity in the tested • All kits are rigorously tested for shipping sample types stability to determine impact on each analyte • Assay specificity is ensured by confirming • Sample stability is examined at a range of consistent performance in singleplex and temperatures, and by freeze/thaw multiplex formats Precision and Accuracy Method Comparisons • Precision of control standards are typically • We test our kits against in-house kits and kits within 15% CV for intra-assay and 20% CV from other vendors when available, ensuring for inter-assay the highest performing kits • Spike recovery in sample is performed for each analyte at three concentrations; accuracy Selectivity is normally within ± 30% • Assay buffers and bead diluents are optimized to enhance antibody binding to only those Linearity of Dilution analytes of interest in samples • Diluted sample results must be directly proportional to the concentration of analyte in Detection and Sensitivity the sample, typically within ± 30% • The assay range and minimum detectible concentrations (minDCs or LLoQ) for each Lot-to-Lot Consistency analyte is published in our protocols, and is based on actual samples • A “gold standard” calibrator is defined during assay development and used as a reference for all future production lots Performance in a Sample Matrix

• Assays are verified for the sample type noted Dedicated Support in the protocol; for serum/plasma sample kits, we provide an optimized native serum matrix • Committed care when and where you need it, to mimic the environment of the sample, from our sales specialists, technical support normalizing assay performance scientists, customer support, and custom immunoassay development team

We’ll help you choose the right platform and assays to meet your needs today, and we’ll partner with you to meet those needs in the future—without compromising quality.

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