Keystone Exams: Biology Assessment Anchors and Eligible Content with Sample Questions and Glossary
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Glossary - Cellbiology
1 Glossary - Cellbiology Blotting: (Blot Analysis) Widely used biochemical technique for detecting the presence of specific macromolecules (proteins, mRNAs, or DNA sequences) in a mixture. A sample first is separated on an agarose or polyacrylamide gel usually under denaturing conditions; the separated components are transferred (blotting) to a nitrocellulose sheet, which is exposed to a radiolabeled molecule that specifically binds to the macromolecule of interest, and then subjected to autoradiography. Northern B.: mRNAs are detected with a complementary DNA; Southern B.: DNA restriction fragments are detected with complementary nucleotide sequences; Western B.: Proteins are detected by specific antibodies. Cell: The fundamental unit of living organisms. Cells are bounded by a lipid-containing plasma membrane, containing the central nucleus, and the cytoplasm. Cells are generally capable of independent reproduction. More complex cells like Eukaryotes have various compartments (organelles) where special tasks essential for the survival of the cell take place. Cytoplasm: Viscous contents of a cell that are contained within the plasma membrane but, in eukaryotic cells, outside the nucleus. The part of the cytoplasm not contained in any organelle is called the Cytosol. Cytoskeleton: (Gk. ) Three dimensional network of fibrous elements, allowing precisely regulated movements of cell parts, transport organelles, and help to maintain a cell’s shape. • Actin filament: (Microfilaments) Ubiquitous eukaryotic cytoskeletal proteins (one end is attached to the cell-cortex) of two “twisted“ actin monomers; are important in the structural support and movement of cells. Each actin filament (F-actin) consists of two strands of globular subunits (G-Actin) wrapped around each other to form a polarized unit (high ionic cytoplasm lead to the formation of AF, whereas low ion-concentration disassembles AF). -
Exogenous Antigens Gain Access to the Major Histocompatibility Complex Class I Processing Pathway in B Cells by Receptor-Mediated Uptake Byyong Ke and Judith A
Brief Definitive Report Exogenous Antigens Gain Access to the Major Histocompatibility Complex Class I Processing Pathway in B Cells by Receptor-mediated Uptake ByYong Ke and Judith A. Kapp From the Department of Pathology and Winship Cancer Center, Emory University School of Medicine, Atlanta, Georgia 30322 Summary Professional antigen-presenting cells, such as macrophages, dendritic cells, or B cells, take up soluble, exogenous antigens (Ags) and process them through the class II pathway, Several re- ports have shown that phagocytic macrophages also process particulate or soluble forms of ex- ogenous Ag via the class I pathway. By contrast, B cells normally do not process soluble, exog- enous Ag by way of the class I pathway unless Ags are directly introduced into the cytoplasm. Here we report that B cells present exogenous Ag via the class I pathway when Ags are taken up by receptor-mediated endocytosis. Thus, specialized methods of Ag uptake such as phago- cytosis or receptor-mediated endocytosis deliver exogenous Ag into the class I pathway of Ag processing and presentation. xogenous Ags are taken up nonspecifically by fluid- Materials and Methods phase pinocytosis or endocytosis, processed, and pre- E Antigens and Reagents. Chicken egg OVA, TNP, chloroquine, sented via class II pathway by professional APCs, such as brefeldin A, and phenazine methosulfate (PMS) were purchased macrophages, dendritic cells, or B cells. Exogenous Ags do from Sigma Chemical Co. (St. Louis, MO). Crystallized beef in- not enter the class I pathway of most cells (1-3). However, sulin was purchased from Lilly Research Labs (Indianapolis, IN). a small population of phagocytic macrophages can process TNP was conjugated to OVA or insulin as described (13). -
Revised Glossary for AQA GCSE Biology Student Book
Biology Glossary amino acids small molecules from which proteins are A built abiotic factor physical or non-living conditions amylase a digestive enzyme (carbohydrase) that that affect the distribution of a population in an breaks down starch ecosystem, such as light, temperature, soil pH anaerobic respiration respiration without using absorption the process by which soluble products oxygen of digestion move into the blood from the small intestine antibacterial chemicals chemicals produced by plants as a defence mechanism; the amount abstinence method of contraception whereby the produced will increase if the plant is under attack couple refrains from intercourse, particularly when an egg might be in the oviduct antibiotic e.g. penicillin; medicines that work inside the body to kill bacterial pathogens accommodation ability of the eyes to change focus antibody protein normally present in the body acid rain rain water which is made more acidic by or produced in response to an antigen, which it pollutant gases neutralises, thus producing an immune response active site the place on an enzyme where the antimicrobial resistance (AMR) an increasing substrate molecule binds problem in the twenty-first century whereby active transport in active transport, cells use energy bacteria have evolved to develop resistance against to transport substances through cell membranes antibiotics due to their overuse against a concentration gradient antiretroviral drugs drugs used to treat HIV adaptation features that organisms have to help infections; they -
The Internal Environment of Animals: 32 Organization and Regulation
CHAPTER The Internal Environment of Animals: 32 Organization and Regulation KEY CONCEPTS 32.1 Animal form and function are correlated at all levels of organization 32.2 The endocrine and nervous systems act individually and together in regulating animal physiology 32.3 Feedback control maintains the internal environment in many animals 32.4 A shared system mediates osmoregulation and excretion in many animals 32.5 The mammalian kidney’s ability to conserve water is a key terrestrial adaptation ▲ Figure 32.1 How do long legs help this scavenger survive in the scorching desert heat? Diverse Forms, Common Challenges Because form and function are correlated, examining anat- omy often provides clues to physiology—biological function. he desert ant (Cataglyphis) in Figure 32.1 scavenges In the case of the desert ant, researchers noted that its stilt-like insects that have succumbed to the daytime heat of the legs are disproportionately long, elevating the rest of the ant Sahara Desert. To gather corpses for feeding, the ant 4 mm above the sand. At this height, the ant’s body is exposed Tmust forage when surface temperatures on the sunbaked sand to a temperature 6°C lower than that at ground level. The ant’s exceed 60°C (140°F), well above the thermal limit for virtually long legs also facilitate rapid locomotion: Researchers have all animals. How does the desert ant survive these conditions? found that desert ants can run as fast as 1 m/sec, close to the To address this question, we need to consider the relationship top speed recorded for a running arthropod. -
Human Anatomy (Biology 2) Lecture Notes Updated July 2017 Instructor
Human Anatomy (Biology 2) Lecture Notes Updated July 2017 Instructor: Rebecca Bailey 1 Chapter 1 The Human Body: An Orientation • Terms - Anatomy: the study of body structure and relationships among structures - Physiology: the study of body function • Levels of Organization - Chemical level 1. atoms and molecules - Cells 1. the basic unit of all living things - Tissues 1. cells join together to perform a particular function - Organs 1. tissues join together to perform a particular function - Organ system 1. organs join together to perform a particular function - Organismal 1. the whole body • Organ Systems • Anatomical Position • Regional Names - Axial region 1. head 2. neck 3. trunk a. thorax b. abdomen c. pelvis d. perineum - Appendicular region 1. limbs • Directional Terms - Superior (above) vs. Inferior (below) - Anterior (toward the front) vs. Posterior (toward the back)(Dorsal vs. Ventral) - Medial (toward the midline) vs. Lateral (away from the midline) - Intermediate (between a more medial and a more lateral structure) - Proximal (closer to the point of origin) vs. Distal (farther from the point of origin) - Superficial (toward the surface) vs. Deep (away from the surface) • Planes and Sections divide the body or organ - Frontal or coronal 1. divides into anterior/posterior 2 - Sagittal 1. divides into right and left halves 2. includes midsagittal and parasagittal - Transverse or cross-sectional 1. divides into superior/inferior • Body Cavities - Dorsal 1. cranial cavity 2. vertebral cavity - Ventral 1. lined with serous membrane 2. viscera (organs) covered by serous membrane 3. thoracic cavity a. two pleural cavities contain the lungs b. pericardial cavity contains heart c. the cavities are defined by serous membrane d. -
CHAPTER 1 Functional Organization of the Human Body and Control of the “Internal Environment”
CHAPTER 1 Functional Organization of the Human Body and Control of the “Internal Environment” Physiology is the science that seeks to understand the function of living organisms and their parts. In human physiology, we are concerned with the characteristics of the human body that allow us to sense our environment, move about, think and communicate, reproduce, and perform all of the functions that enable us to survive and thrive as living beings. Human physiology links the basic sciences with clin- ical medicine and integrates multiple functions of mol- ecules and subcellular components, the cells, tissues, and organs into the functions of the living human being. This integration requires communication and coordina- tion by a vast array of control systems that operate at every level, from the genes that program synthesis of molecules to the complex nervous and hormonal sys- tems that coordinate functions of cells, tissues, and organs throughout the body. Life in the human being relies on this total function, which is considerably more complex than the sum of the functions of the individual cells, tissues, and organs. Cells Are the Living Units of the Body. Each organ is an aggregate of many cells held together by intercellu- lar supporting structures. The entire body contains 35 to 40 trillion cells, each of which is adapted to perform special functions. These individual cell functions are coordinated by multiple regulatory systems operating in cells, tissues, organs, and organ systems. Although the many cells of the body differ from each other in their special functions, all of them have certain basic characteristics. -
Introduction to the Cell Cell History Cell Structures and Functions
Introduction to the cell cell history cell structures and functions CK-12 Foundation December 16, 2009 CK-12 Foundation is a non-profit organization with a mission to reduce the cost of textbook materials for the K-12 market both in the U.S. and worldwide. Using an open-content, web-based collaborative model termed the “FlexBook,” CK-12 intends to pioneer the generation and distribution of high quality educational content that will serve both as core text as well as provide an adaptive environment for learning. Copyright ©2009 CK-12 Foundation This work is licensed under the Creative Commons Attribution-Share Alike 3.0 United States License. To view a copy of this license, visit http://creativecommons.org/licenses/by-sa/3.0/us/ or send a letter to Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA. Contents 1 Cell structure and function dec 16 5 1.1 Lesson 3.1: Introduction to Cells .................................. 5 3 www.ck12.org www.ck12.org 4 Chapter 1 Cell structure and function dec 16 1.1 Lesson 3.1: Introduction to Cells Lesson Objectives • Identify the scientists that first observed cells. • Outline the importance of microscopes in the discovery of cells. • Summarize what the cell theory proposes. • Identify the limitations on cell size. • Identify the four parts common to all cells. • Compare prokaryotic and eukaryotic cells. Introduction Knowing the make up of cells and how cells work is necessary to all of the biological sciences. Learning about the similarities and differences between cell types is particularly important to the fields of cell biology and molecular biology. -
Bioenergetics and Metabolism Mitochondria Chloroplasts
Bioenergetics and metabolism Mitochondria Chloroplasts Peroxisomes B. Balen Chemiosmosis common pathway of mitochondria, chloroplasts and prokaryotes to harness energy for biological purposes → chemiosmotic coupling – ATP synthesis (chemi) + membrane transport (osmosis) Prokaryotes – plasma membrane → ATP production Eukaryotes – plasma membrane → transport processes – membranes of cell compartments – energy-converting organelles → production of ATP • Mitochondria – fungi, animals, plants • Plastids (chloroplasts) – plants The essential requirements for chemiosmosis source of high-energy e- membrane with embedded proton pump and ATP synthase energy from sunlight or the pump harnesses the energy of e- transfer to pump H+→ oxidation of foodstuffs is proton gradient across the membrane used to create H+ gradient + across a membrane H gradient serves as an energy store that can be used to drive ATP synthesis Figures 14-1; 14-2 Molecular Biology of the Cell (© Garland Science 2008) Electron transport processes (A) mitochondrion converts energy from chemical fuels (B) chloroplast converts energy from sunlight → electron-motive force generated by the 2 photosystems enables the chloroplast to drive electron transfer from H2O to carbohydrate → chloroplast electron transfer is opposite of electron transfer in a mitochondrion Figure 14-3 Molecular Biology of the Cell (© Garland Science 2008) Carbohydrate molecules and O2 are products of the chloroplast and inputs for the mitochondrion Figure 2-41; 2-76 Molecular Biology of the Cell (© Garland -
1 [ Reading for Lecture 7] (1) 2Nd Law of Thermodynamics: Entropy
[ Reading for lecture 7] (1) 2nd law of thermodynamics: Entropy Increases Life Decreases it’s own entropy – at the expense of the rest of the universe Most globally – takes photons (low entropy – straight line !) and converts them ultimately into heat (high entropy), with all of life in- between. To do this, life needs to gather, store and manipulate sources of Free-Energy Free energy can be thought of as the “currency” of life. Any reaction that requires free energy input (eg. making DNA from nucleic acids, doing mechanical work, building a protonmotive force) must be “paid for” by coupling to a reaction that releases free energy. This lecture: Types of biological free-energy, ways and mechanisms in which they are interconverted. These processes are essentially what life is. 1 Types of biological free-energy 2 Protonmotive force (pmf) [Protonmotive Force] (3) Electrical potential plus concentration gradient, H+ or “protons”. (see lecture 6) nb. Nernst potential is the voltage when pmf is zero, at equilibrium. Pmf is a measure of how far from equilibrium the membrane is –the “driving force” for proton transport across the membrane. Generated by active transport of protons across the membrane Free-energy sources: absorption of photons, break-down of food. pH gradient (chemical potential) is necessary if the pmf is to do significant work Very few protons need to be pumped to establish the membrane voltage, BUT… Just like charging a battery, you need to provide current as well as voltage. pH gradient also increases the free energy per proton –diffusion as well as voltage drives protons. -
Cell Growth-Regulated Expression of Mammalian MCM5 and MCM6 Genes Mediated by the Transcription Factor E2F
Oncogene (1999) 18, 2299 ± 2309 ã 1999 Stockton Press All rights reserved 0950 ± 9232/99 $12.00 http://www.stockton-press.co.uk/onc Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F Kiyoshi Ohtani1, Ritsuko Iwanaga1, Masataka Nakamura*,1, Masa-aki Ikeda2, Norikazu Yabuta3, Hiromichi Tsuruga3 and Hiroshi Nojima3 1Human Gene Sciences Center, Tokyo Medical and Dental University, Tokyo 113-8510, Japan 2Department of Developmental Biology, Graduate School of Dentistry, Tokyo Medical and Dental University, Tokyo 113-8549, Japan; 3Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan Initiation of DNA replication requires the function of family (MCM2-7) that have been identi®ed in yeast, MCM gene products, which participate in ensuring that Xenopus, and human. Mcm proteins seem to regulate DNA replication occurs only once in the cell cycle. the initiation at the replication origin where the loading Expression of all mammalian genes of the MCM family of the proteins onto the origin recognition complex is induced by growth stimulation, unlike yeast, and the (ORC) is regulated by Cdc6 and cyclin-dependent mRNA levels peak at G1/S boundary. In this study, we kinases (Donovan et al., 1997; Tanaka et al., 1997). examined the transcriptional activities of isolated human However, the mechanism(s) by which Mcm proteins MCM gene promoters. Human MCM5 and MCM6 control the initiation of DNA replication remains promoters with mutation in the E2F sites failed in unclear. promoter regulation following serum stimulation and Xenopus Mcm proteins seem to be able to access exogenous E2F expression. -
Study Guide Medical Terminology by Thea Liza Batan About the Author
Study Guide Medical Terminology By Thea Liza Batan About the Author Thea Liza Batan earned a Master of Science in Nursing Administration in 2007 from Xavier University in Cincinnati, Ohio. She has worked as a staff nurse, nurse instructor, and level department head. She currently works as a simulation coordinator and a free- lance writer specializing in nursing and healthcare. All terms mentioned in this text that are known to be trademarks or service marks have been appropriately capitalized. Use of a term in this text shouldn’t be regarded as affecting the validity of any trademark or service mark. Copyright © 2017 by Penn Foster, Inc. All rights reserved. No part of the material protected by this copyright may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without permission in writing from the copyright owner. Requests for permission to make copies of any part of the work should be mailed to Copyright Permissions, Penn Foster, 925 Oak Street, Scranton, Pennsylvania 18515. Printed in the United States of America CONTENTS INSTRUCTIONS 1 READING ASSIGNMENTS 3 LESSON 1: THE FUNDAMENTALS OF MEDICAL TERMINOLOGY 5 LESSON 2: DIAGNOSIS, INTERVENTION, AND HUMAN BODY TERMS 28 LESSON 3: MUSCULOSKELETAL, CIRCULATORY, AND RESPIRATORY SYSTEM TERMS 44 LESSON 4: DIGESTIVE, URINARY, AND REPRODUCTIVE SYSTEM TERMS 69 LESSON 5: INTEGUMENTARY, NERVOUS, AND ENDOCRINE S YSTEM TERMS 96 SELF-CHECK ANSWERS 134 © PENN FOSTER, INC. 2017 MEDICAL TERMINOLOGY PAGE III Contents INSTRUCTIONS INTRODUCTION Welcome to your course on medical terminology. You’re taking this course because you’re most likely interested in pursuing a health and science career, which entails proficiencyincommunicatingwithhealthcareprofessionalssuchasphysicians,nurses, or dentists. -
Macromolecules of Life Worksheet
Test Your Knowledge: Macromolecules of Life For Anatomy & Physiology I Biological Macromolecules, Fill in the blank: Macromolecule Monomer Elements Present Function Examples Carbohydrates C,H,O Lipids Fats, Oils, Phospholipids, Cholesterol, Grease, Waxes, Steroids Proteins Amino Acids Nucleic Acids Genetic Information Carbohydrates are classified by __________. The most common simple sugars are glucose, galactose and fructose that are made of a single sugar molecule. These can be classified as __________. Sucrose and __________ are classified as disaccharides; they are made of two monosaccharides joined by a dehydration reaction. The most complex carbohydrates are starch, __________ and cellulose, classified as __________. Lipids most abundant form are __________. Triglycerides building blocks are 1 __________ and 3 __________ per molecule. If a triglyceride only contains __________ bonds that contain the maximum number of __________, then it is classified as a saturated fat. If a triglyceride contains one or more __________ bonds, then it is classified as an unsaturated fat. Lipids are also responsible for a major component of the cell membrane wall that is both attracted to and repelled by water, called __________. The tail of this structure is made of 2 __________, that are water insoluble (hydrophobic). The head of this structure is made of a single __________, that is water soluble (hydrophilic). Proteins building blocks are amino acids that are held together with __________ bonds. These are covalent bonds that link the amino end of one amino acid with the carboxyl end of another. Their overall shape determines their __________. The complex 3D shape of a protein is called a __________.