CSTF1 Polyclonal Antibody Mammalian G Protein Beta Subunits, This Protein Contains Transducin-Like Repeats

Total Page：16

File Type：pdf, Size：1020Kb

CSTF1 polyclonal antibody mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript Catalog Number: PAB17377 variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by Regulatory Status: For research use only (RUO) RefSeq] Product Description: Rabbit polyclonal antibody raised References: against synthetic peptide of CSTF1. 1. The DNA sequence and comparative analysis of human chromosome 20. Deloukas P, Matthews LH, Immunogen: A synthetic peptide corresponding to Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, N-terminus of human CSTF1. Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird Host: Rabbit CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Reactivity: Human,Mouse,Rat Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg Applications: WB-Ce S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson (See our web site product page for detailed applications A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington information) AG, Frankland JA, Fraser A, French L, Garner P, Grafh Nature. 2001 Dec 20-27;414(6866):865-71. Protocols: See our web site at 2. Functional interaction of BRCA1-associated BARD1 http://www.abnova.com/support/protocols.asp or product with polyadenylation factor CstF-50. Kleiman FE, Manley page for detailed protocols JL. Science. 1999 Sep 3;285(5433):1576-9. 3. The human 64-kDa polyadenylylation factor contains Specificity: This antibody detects endogenous levels of a ribonucleoprotein-type RNA binding domain and total CSTF1 protein. unusual auxiliary motifs. Takagaki Y, MacDonald CC, Shenk T, Manley JL. Proc Natl Acad Sci U S A. 1992 Form: Liquid Feb 15;89(4):1403-7. Recommend Usage: Western Blot (1:500-1:1000) ELISA (1:20000) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol) Storage Instruction: Store at -20°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 1477 Gene Symbol: CSTF1 Gene Alias: CstF-50, CstFp50 Gene Summary: This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to Page 1/1 Powered by TCPDF (www.tcpdf.org).

Copy Link

Recommended publications

Essential Genes and Their Role in Autism Spectrum Disorder
University of Pennsylvania ScholarlyCommons Publicly Accessible Penn Dissertations 2017 Essential Genes And Their Role In Autism Spectrum Disorder Xiao Ji University of Pennsylvania, [email protected] Follow this and additional works at: https://repository.upenn.edu/edissertations Part of the Bioinformatics Commons, and the Genetics Commons Recommended Citation Ji, Xiao, "Essential Genes And Their Role In Autism Spectrum Disorder" (2017). Publicly Accessible Penn Dissertations. 2369. https://repository.upenn.edu/edissertations/2369 This paper is posted at ScholarlyCommons. https://repository.upenn.edu/edissertations/2369 For more information, please contact [email protected]. Essential Genes And Their Role In Autism Spectrum Disorder Abstract Essential genes (EGs) play central roles in fundamental cellular processes and are required for the survival of an organism. EGs are enriched for human disease genes and are under strong purifying selection. This intolerance to deleterious mutations, commonly observed haploinsufficiency and the importance of EGs in pre- and postnatal development suggests a possible cumulative effect of deleterious variants in EGs on complex neurodevelopmental disorders. Autism spectrum disorder (ASD) is a heterogeneous, highly heritable neurodevelopmental syndrome characterized by impaired social interaction, communication and repetitive behavior. More and more genetic evidence points to a polygenic model of ASD and it is estimated that hundreds of genes contribute to ASD. The central question addressed in this dissertation is whether genes with a strong effect on survival and fitness (i.e. EGs) play a specific oler in ASD risk. I compiled a comprehensive catalog of 3,915 mammalian EGs by combining human orthologs of lethal genes in knockout mice and genes responsible for cell-based essentiality.
[Show full text]
Investigating the Molecular Mechanisms of Splicing-Perturbing Small Molecules with Massively Parallel Sequencing in a Myotonic Dystrophy 1 Model
INVESTIGATING THE MOLECULAR MECHANISMS OF SPLICING-PERTURBING SMALL MOLECULES WITH MASSIVELY PARALLEL SEQUENCING IN A MYOTONIC DYSTROPHY 1 MODEL by MATTHEW TANNER A THESIS Presented to the Department of Chemistry and Biochemistry and the Robert D. Clark Honors College in partial fulfillment of the requirements for the degree of Bachelor of Science June, 2014 An Abstract of the Thesis of Matthew Tanner for the degree of Bachelor of Science in the Department of Chemistry and Biochemistry to be taken June, 2014 Title: Investigating the Molecular Mechanisms of Splicing-Perturbing Small Molecules with Massively Parallel Sequencing in a Myotonic Dystrophy 1 Model Approved: 4 fhh 73cJ-i J. Andrew Berglund Myotonic dystrophy is the most common form of adult-onset muscular dystrophy and appears in two forms: myotonic dystrophy 1 (OM 1) and 2 (DM2). Both diseases arc characterized by progressive muscle degeneration, myotonia. iridescent cataracts, and in severe cases neurodcgcncration and cardiac dysfunction. Both fonns of myotonic dystrophy arc caused by an expansion of repeat DNA in distinct loci in the genome. In DM 1, a CTG repeat is expanded from less than 50 repeats in nonnal individuals to up to several thousand repeats in DM 1 patients. The molecular basis of this disease relics on the transcription of these repeats from DNA into RNA. Small molecules that can specifically target the repeats at the DNA level and inhibit their transcription - and thus alleviate the disease symptoms - represent a prime target for the development of therapeutics. This study investigates the capacity of two small molecules, pentamidine and actinomycin D, to reverse the molecular symptoms of DM I through transcriptional inhibition and provides insight into their DNA target specificity and potential mechanisms of action.
[Show full text]
3' UTR-Isoform Choice Has Limited Influence on the Stability and Translational Efficiency of Most Mrnas in Mouse Fibroblasts
Downloaded from genome.cshlp.org on September 23, 2021 - Published by Cold Spring Harbor Laboratory Press 3' UTR-isoform choice has limited influence on the stability and translational efficiency of most mRNAs in mouse fibroblasts Noah Spies1,2,3,5, Christopher B. Burge1,4, David P. Bartel1,2,3 September 25, 2013 1Department of Biology, 2Howard Hughes Medical Institute, 3Whitehead Institute for Biomedical Research Cambridge, MA 02142 4Department of Biological Engineering Massachusetts Institute of Technology Cambridge, MA 02139 USA 5Current address: Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305 Address correspondence to: [email protected] [email protected] Running title: Stability and translation of 3' UTR isoforms Downloaded from genome.cshlp.org on September 23, 2021 - Published by Cold Spring Harbor Laboratory Press Abstract Variation in protein output across the genome is controlled at several levels, but the relative contributions of different regulatory mechanisms remain poorly understood. Here, we obtained global measurements of decay and translation rates for mRNAs with alternative 3' untranslated regions (3' UTRs) in murine 3T3 cells. Distal tandem isoforms had slightly but significantly lower mRNA stability and greater translational efficiency than proximal isoforms on average. The diversity of alternative 3' UTRs also enabled inference and evaluation of both positively and negatively acting cis regulatory elements. The 3' UTR elements with the greatest implied influence were microRNA complementary sites, which were associated with repression of 32% and 4% at the stability and translational levels, respectively. Nonetheless, both the decay and translation rates were highly correlated for proximal and distal 3' UTR isoforms from the same genes, implying that in 3T3 cells alternative 3' UTR sequences play a surprisingly small regulatory role compared to other mRNA regions.
[Show full text]
Antisense Oligonucleotide-Based Therapeutic Against Menin for Triple-Negative Breast Cancer Treatment
biomedicines Article Antisense Oligonucleotide-Based Therapeutic against Menin for Triple-Negative Breast Cancer Treatment Dang Tan Nguyen 1,†, Thi Khanh Le 1,2,† , Clément Paris 1,†, Chaïma Cherif 1 , Stéphane Audebert 3 , Sandra Oluchi Udu-Ituma 1,Sébastien Benizri 4 , Philippe Barthélémy 4 , François Bertucci 1, David Taïeb 1,5 and Palma Rocchi 1,* 1 Predictive Oncology Laboratory, Centre de Recherche en Cancérologie de Marseille (CRCM), Inserm UMR 1068, CNRS UMR 7258, Institut Paoli-Calmettes, Aix-Marseille University, 27 Bd. Leï Roure, 13273 Marseille, France; [email protected] (D.T.N.); [email protected] (T.K.L.); [email protected] (C.P.); [email protected] (C.C.); [email protected] (S.O.U.-I.); [email protected] (F.B.); [email protected] (D.T.) 2 Department of Life Science, University of Science and Technology of Hanoi (USTH), Hanoi 000084, Vietnam 3 Marseille Protéomique, Centre de Recherche en Cancérologie de Marseille, INSERM, CNRS, Institut Paoli-Calmettes, Aix-Marseille University, 13009 Marseille, France; [email protected] 4 ARNA Laboratory, INSERM U1212, CNRS UMR 5320, University of Bordeaux, 33076 Bordeaux, France; [email protected] (S.B.); [email protected] (P.B.) 5 Biophysics and Nuclear Medicine Department, La Timone University Hospital, European Center for Research in Medical Imaging, Aix-Marseille University, 13005 Marseille, France * Correspondence: [email protected]; Tel.: +33-626-941-287 † These authors contributed equally. Citation: Nguyen, D.T.; Le, T.K.; Paris, C.; Cherif, C.; Audebert, S.; Abstract: The tumor suppressor menin has dual functions, acting either as a tumor suppressor or Oluchi Udu-Ituma, S.; Benizri, S.; as an oncogene/oncoprotein, depending on the oncological context.
[Show full text]
The DNA Sequence and Comparative Analysis of Human Chromosome 20
articles The DNA sequence and comparative analysis of human chromosome 20 P. Deloukas, L. H. Matthews, J. Ashurst, J. Burton, J. G. R. Gilbert, M. Jones, G. Stavrides, J. P. Almeida, A. K. Babbage, C. L. Bagguley, J. Bailey, K. F. Barlow, K. N. Bates, L. M. Beard, D. M. Beare, O. P. Beasley, C. P. Bird, S. E. Blakey, A. M. Bridgeman, A. J. Brown, D. Buck, W. Burrill, A. P. Butler, C. Carder, N. P. Carter, J. C. Chapman, M. Clamp, G. Clark, L. N. Clark, S. Y. Clark, C. M. Clee, S. Clegg, V. E. Cobley, R. E. Collier, R. Connor, N. R. Corby, A. Coulson, G. J. Coville, R. Deadman, P. Dhami, M. Dunn, A. G. Ellington, J. A. Frankland, A. Fraser, L. French, P. Garner, D. V. Grafham, C. Grif®ths, M. N. D. Grif®ths, R. Gwilliam, R. E. Hall, S. Hammond, J. L. Harley, P. D. Heath, S. Ho, J. L. Holden, P. J. Howden, E. Huckle, A. R. Hunt, S. E. Hunt, K. Jekosch, C. M. Johnson, D. Johnson, M. P. Kay, A. M. Kimberley, A. King, A. Knights, G. K. Laird, S. Lawlor, M. H. Lehvaslaiho, M. Leversha, C. Lloyd, D. M. Lloyd, J. D. Lovell, V. L. Marsh, S. L. Martin, L. J. McConnachie, K. McLay, A. A. McMurray, S. Milne, D. Mistry, M. J. F. Moore, J. C. Mullikin, T. Nickerson, K. Oliver, A. Parker, R. Patel, T. A. V. Pearce, A. I. Peck, B. J. C. T. Phillimore, S. R. Prathalingam, R. W. Plumb, H. Ramsay, C. M.
[Show full text]
NRF1) Coordinates Changes in the Transcriptional and Chromatin Landscape Affecting Development and Progression of Invasive Breast Cancer
Florida International University FIU Digital Commons FIU Electronic Theses and Dissertations University Graduate School 11-7-2018 Decipher Mechanisms by which Nuclear Respiratory Factor One (NRF1) Coordinates Changes in the Transcriptional and Chromatin Landscape Affecting Development and Progression of Invasive Breast Cancer Jairo Ramos [email protected] Follow this and additional works at: https://digitalcommons.fiu.edu/etd Part of the Clinical Epidemiology Commons Recommended Citation Ramos, Jairo, "Decipher Mechanisms by which Nuclear Respiratory Factor One (NRF1) Coordinates Changes in the Transcriptional and Chromatin Landscape Affecting Development and Progression of Invasive Breast Cancer" (2018). FIU Electronic Theses and Dissertations. 3872. https://digitalcommons.fiu.edu/etd/3872 This work is brought to you for free and open access by the University Graduate School at FIU Digital Commons. It has been accepted for inclusion in FIU Electronic Theses and Dissertations by an authorized administrator of FIU Digital Commons. For more information, please contact [email protected]. FLORIDA INTERNATIONAL UNIVERSITY Miami, Florida DECIPHER MECHANISMS BY WHICH NUCLEAR RESPIRATORY FACTOR ONE (NRF1) COORDINATES CHANGES IN THE TRANSCRIPTIONAL AND CHROMATIN LANDSCAPE AFFECTING DEVELOPMENT AND PROGRESSION OF INVASIVE BREAST CANCER A dissertation submitted in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY in PUBLIC HEALTH by Jairo Ramos 2018 To: Dean Tomás R. Guilarte Robert Stempel College of Public Health and Social Work This dissertation, Written by Jairo Ramos, and entitled Decipher Mechanisms by Which Nuclear Respiratory Factor One (NRF1) Coordinates Changes in the Transcriptional and Chromatin Landscape Affecting Development and Progression of Invasive Breast Cancer, having been approved in respect to style and intellectual content, is referred to you for judgment.
[Show full text]
Uncovering RNA Binding Proteins Associated with Age and Gender During Liver Maturation
OPEN Uncovering RNA binding proteins SUBJECT AREAS: associated with age and gender during GENOME-WIDE ASSOCIATION STUDIES liver maturation REGULATORY NETWORKS Praneet Chaturvedi1, Yaseswini Neelamraju1, Waqar Arif2, Auinash Kalsotra2 & Sarath Chandra Janga1,3,4 AGEING GENE REGULATORY NETWORKS 1Department of BioHealth Informatics, School of Informatics and Computing, Indiana University Purdue University, 719 Indiana Ave Ste 319, Walker Plaza Building, Indianapolis, Indiana 46202, 2Departments of Biochemistry and Medical Biochemistry, University Received of Illinois, Urbana-Champaign, Illinois 61801, USA, 3Center for Computational Biology and Bioinformatics, Indiana University 11 December 2014 School of Medicine, 5021 Health Information and Translational Sciences (HITS), 410 West 10th Street, Indianapolis, Indiana, 46202, 4Department of Medical and Molecular Genetics, Indiana University School of Medicine, Medical Research and Library Accepted Building, 975 West Walnut Street, Indianapolis, Indiana, 46202. 9 March 2015 Published In the present study, we perform an association analysis focusing on the expression changes of 1344 RNA 31 March 2015 Binding proteins (RBPs) as a function of age and gender in human liver. We identify 88 and 45 RBPs to be significantly associated with age and gender respectively. Experimental verification of several of the predicted associations in mice confirmed our findings. Our results suggest that a small fraction of the gender-associated RBPs (,40%) are expressed higher in males than females. Altogether, these observations Correspondence and show that several of these RBPs are important and conserved regulators in maintaining liver function. requests for materials Further analysis of the protein interaction network of RBPs associated with age and gender based on the should be addressed to centrality measures like degree, betweenness and closeness revealed that several of these RBPs might be S.C.J.
[Show full text]
1 SUPPLEMENTAL DATA Figure S1. Poly I:C Induces IFN-Β Expression
SUPPLEMENTAL DATA Figure S1. Poly I:C induces IFN-β expression and signaling. Fibroblasts were incubated in media with or without Poly I:C for 24 h. RNA was isolated and processed for microarray analysis. Genes showing >2-fold up- or down-regulation compared to control fibroblasts were analyzed using Ingenuity Pathway Analysis Software (Red color, up-regulation; Green color, down-regulation). The transcripts with known gene identifiers (HUGO gene symbols) were entered into the Ingenuity Pathways Knowledge Base IPA 4.0. Each gene identifier mapped in the Ingenuity Pathways Knowledge Base was termed as a focus gene, which was overlaid into a global molecular network established from the information in the Ingenuity Pathways Knowledge Base. Each network contained a maximum of 35 focus genes. 1 Figure S2. The overlap of genes regulated by Poly I:C and by IFN. Bioinformatics analysis was conducted to generate a list of 2003 genes showing >2 fold up or down- regulation in fibroblasts treated with Poly I:C for 24 h. The overlap of this gene set with the 117 skin gene IFN Core Signature comprised of datasets of skin cells stimulated by IFN (Wong et al, 2012) was generated using Microsoft Excel. 2 Symbol Description polyIC 24h IFN 24h CXCL10 chemokine (C-X-C motif) ligand 10 129 7.14 CCL5 chemokine (C-C motif) ligand 5 118 1.12 CCL5 chemokine (C-C motif) ligand 5 115 1.01 OASL 2'-5'-oligoadenylate synthetase-like 83.3 9.52 CCL8 chemokine (C-C motif) ligand 8 78.5 3.25 IDO1 indoleamine 2,3-dioxygenase 1 76.3 3.5 IFI27 interferon, alpha-inducible
[Show full text]
Dualistic Role of BARD1 in Cancer
G C A T T A C G G C A T genes Review Dualistic Role of BARD1 in Cancer Flora Cimmino 1,2,*, Daniela Formicola 3 and Mario Capasso 1,3 ID 1 Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università Degli Studi di Napoli “Federico II”, 80131 Naples, Italy; [email protected] 2 CEINGE Biotecnologie Avanzate, 80131 Naples, Italy 3 IRCCS SDN, Istituto di Ricerca Diagnostica e Nucleare, 80143 Naples, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-081-373-7736; Fax: +39-081-373-7804 Received: 24 October 2017; Accepted: 1 December 2017; Published: 8 December 2017 Abstract: BRCA1 Associated RING Domain 1 (BARD1) encodes a protein which interacts with the N-terminal region of BRCA1 in vivo and in vitro. The full length (FL) BARD1 mRNA includes 11 exons and encodes a protein comprising of six domains (N-terminal RING-ﬁnger domain, three Ankyrin repeats and two C-terminal BRCT domains) with different functions. Emerging data suggest that BARD1 can have both tumor-suppressor gene and oncogene functions in tumor initiation and progression. Indeed, whereas FL BARD1 protein acts as tumor-suppressor with and without BRCA1 interactions, aberrant splice variants of BARD1 have been detected in various cancers and have been shown to play an oncogenic role. Further evidence for a dualistic role came with the identiﬁcation of BARD1 as a neuroblastoma predisposition gene in our genome wide association study which has demonstrated that single nucleotide polymorphisms in BARD1 can correlate with risk or can protect against cancer based on their association with the expression of FL and splice variants of BARD1.
[Show full text]
Mechanisms of Deadenylation Regulation Under Different Cellular Conditions
City University of New York (CUNY) CUNY Academic Works All Dissertations, Theses, and Capstone Projects Dissertations, Theses, and Capstone Projects 2-2014 Mechanisms Of Deadenylation Regulation Under Different Cellular Conditions Xiaokan Zhang Graduate Center, City University of New York How does access to this work benefit ou?y Let us know! More information about this work at: https://academicworks.cuny.edu/gc_etds/135 Discover additional works at: https://academicworks.cuny.edu This work is made publicly available by the City University of New York (CUNY). Contact: [email protected] MECHANISMS OF DEADENYLATION REGULATION UNDER DIFFERENT CELLULAR CONDITIONS by XIAOKAN ZHANG A dissertation submitted to the Graduate Faculty in Biochemistry in partial fulfillment of the requirements for the degree of Doctor of Philosophy, The City University of New York 2014 ii 2014 XIAOKAN ZHANG All Rights Reserved iii This manuscript has been read and accepted for the Graduate Faculty in Biochemistry in satisfaction of the dissertation requirement for the degree of Doctor of Philosophy. Dr. Frida Kleiman, Hunter College of CUNY ____________ __________________________________________________ Date Chair of Examining Committee Dr. Edward Kennelly, The Graduate Center of CUNY ____________ __________________________________________________ Date Executive Officer Dr. Diego Loayza, Hunter College of CUNY Dr. Kevin Ryan, City College of CUNY Dr. Serafin Piñol-Roma, City College of CUNY Dr Hualin Zhong, Hunter College of CUNY Supervisory Committee THE CITY UNIVERSITY OF NEW YORK iv ABSTRACT Mechanisms of Deadenylation Regulation Under Different Cellular Conditions By Xiaokan Zhang Adviser: Professor Frida Esther Kleiman Control of gene expression by regulating mRNA stability after DNA damage has the potential to contribute to the cells rapid response to stress.
[Show full text]
Quantitative Trait Loci Mapping of Macrophage Atherogenic Phenotypes
QUANTITATIVE TRAIT LOCI MAPPING OF MACROPHAGE ATHEROGENIC PHENOTYPES BRIAN RITCHEY Bachelor of Science Biochemistry John Carroll University May 2009 submitted in partial fulfillment of requirements for the degree DOCTOR OF PHILOSOPHY IN CLINICAL AND BIOANALYTICAL CHEMISTRY at the CLEVELAND STATE UNIVERSITY December 2017 We hereby approve this thesis/dissertation for Brian Ritchey Candidate for the Doctor of Philosophy in Clinical-Bioanalytical Chemistry degree for the Department of Chemistry and the CLEVELAND STATE UNIVERSITY College of Graduate Studies by ______________________________ Date: _________ Dissertation Chairperson, Johnathan D. Smith, PhD Department of Cellular and Molecular Medicine, Cleveland Clinic ______________________________ Date: _________ Dissertation Committee member, David J. Anderson, PhD Department of Chemistry, Cleveland State University ______________________________ Date: _________ Dissertation Committee member, Baochuan Guo, PhD Department of Chemistry, Cleveland State University ______________________________ Date: _________ Dissertation Committee member, Stanley L. Hazen, MD PhD Department of Cellular and Molecular Medicine, Cleveland Clinic ______________________________ Date: _________ Dissertation Committee member, Renliang Zhang, MD PhD Department of Cellular and Molecular Medicine, Cleveland Clinic ______________________________ Date: _________ Dissertation Committee member, Aimin Zhou, PhD Department of Chemistry, Cleveland State University Date of Defense: October 23, 2017 DEDICATION I dedicate this work to my entire family. In particular, my brother Greg Ritchey, and most especially my father Dr. Michael Ritchey, without whose support none of this work would be possible. I am forever grateful to you for your devotion to me and our family. You are an eternal inspiration that will fuel me for the remainder of my life. I am extraordinarily lucky to have grown up in the family I did, which I will never forget.
[Show full text]
(12) Patent Application Publication (10) Pub. No.: US 2016/0264934 A1 GALLOURAKIS Et Al
US 20160264934A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2016/0264934 A1 GALLOURAKIS et al. (43) Pub. Date: Sep. 15, 2016 (54) METHODS FOR MODULATING AND Publication Classification ASSAYING MI6AIN STEM CELL POPULATIONS (51) Int. Cl. CI2N5/0735 (2006.01) (71) Applicants: THE GENERAL, HOSPITAL AOIN I/02 (2006.01) CORPORATION, Boston, MA (US); CI2O I/68 (2006.01) The Regents of the University of GOIN 33/573 (2006.01) California, Oakland, CA (US) CI2N 5/077 (2006.01) CI2N5/0793 (2006.01) (72) Inventors: Cosmas GIALLOURAKIS, Boston, (52) U.S. Cl. MA (US); Alan C. MULLEN, CPC ............ CI2N5/0606 (2013.01); CI2N5/0657 Brookline, MA (US); Yi XING, (2013.01); C12N5/0619 (2013.01); C12O Torrance, CA (US) I/6888 (2013.01); G0IN33/573 (2013.01); A0IN I/0226 (2013.01); C12N 2501/72 (73) Assignees: THE GENERAL, HOSPITAL (2013.01); C12N 2506/02 (2013.01); C12O CORPORATION, Boston, MA (US); 2600/158 (2013.01); C12Y 201/01062 The Regents of the University of (2013.01); C12Y 201/01 (2013.01) California, Oakland, CA (US) (57) ABSTRACT (21) Appl. No.: 15/067,780 The present invention generally relates to methods, assays and kits to maintain a human stem cell population in an (22) Filed: Mar 11, 2016 undifferentiated state by inhibiting the expression or function of METTL3 and/or METTL4, and mA fingerprint methods, assays, arrays and kits to assess the cell state of a human stem Related U.S. Application Data cell population by assessing mA levels (e.g. mA peak inten (60) Provisional application No.
[Show full text]