Isolation and Evaluation of Microalgae Strains from the Northern Territory
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Pediastrum Species (Hydrodictyaceae, Sphaeropleales) in Phytoplankton of Sumin Lake (£Êczna-W£Odawa Lakeland)
Vol. 73, No. 1: 39-46, 2004 ACTA SOCIETATIS BOTANICORUM POLONIAE 39 PEDIASTRUM SPECIES (HYDRODICTYACEAE, SPHAEROPLEALES) IN PHYTOPLANKTON OF SUMIN LAKE (£ÊCZNA-W£ODAWA LAKELAND) AGNIESZKA PASZTALENIEC, MA£GORZATA PONIEWOZIK Department of Botany and Hydrobiology, Catholic University of Lublin C.K. Norwida 4, 20-061 Lublin, Poland e-mail: [email protected] (Received: April 7, 2003. Accepted: July 18, 2003) ABSTRACT During studies of phytoplankton in Sumin Lake (£êczna-W³odawa Lakeland), conducted from May till Sep- tember 2001 and 2002, 15 taxa of the genus Pediastrum (Hydrodictyaceae, Sphaeropleales) were found. Among them there were common species as Pediastrum boryanum, P. duplex, P. tetras and P. simplex, but also rare spe- cies as P. integrum or P. kawraiskyi. An especially interesting species was P. orientale, the taxon that until now has not been noted in phytoplankton of Polish water bodies. The paper gives descriptions of the genus Pediastrum coenobia and physico-chemical conditions of the habitat. The original documentation of Pediastrum taxa is added. KEY WORDS: Pediastrum taxa, Chlorophyta, phytoplankton, £êczna-W³odawa Lakeland. INRTODUCTION rved in palynological preparations (Jankovská and Komá- rek 2000, Komárek and Jankovská 2001; Nielsen and Lakes of £êczna-W³odawa Lakeland are the only group Sørensen 1992). in Poland located beyond the limits of a continental glacier The taxonomical research of the genus Pediastrum was of the last glaciation. The genesis of lakes is still disputa- not conducted in phytoplankton of £êczna-W³odawa Lake- ble, but the most of them have a termo-karst origin (Hara- land lakes. Only some information on occurrence of this simiuk and Wojtanowicz 1998). -
[BIO32] the Development of a Biosensor for the Detection of PS II Herbicides Using Green Microalgae
The 4th Annual Seminar of National Science Fellowship 2004 [BIO32] The development of a biosensor for the detection of PS II herbicides using green microalgae Maizatul Suriza Mohamed, Kamaruzaman Ampon, Ann Anton School of Science and Technology, Universiti Malaysia Sabah, Locked Beg 2073, 88999 Kota Kinabalu, Sabah, Malaysia. Introduction Material & Methods Increasing concern over the presence of herbicides in water body has stimulated Equipments and Chemicals research towards the development of sensitive Fluorometer used was TD700 by Turner method and technology to detect herbicides Designs with 13mm borosilicate cuvettes. residue. Biosensors are particularly of interest Excitation and emission wavelength were for the monitoring of herbicides residue in 340nm-500nm and 665nm. Lamp was water body because various classes of daylight white (185-870nm). Equipment for herbicides have a common biological activity, photographing algae was Nikon which can potentially be used for their Photomicrographic Equipment, Model HIII detection. The most important herbicides are (Eclipse 400 Microscope and 35 mm film the photosystem II herbicide group that photomicrography; prism swing type, inhibits PSII electron transfer at the quinone automatic expose and built-in shutter). binding site resulting in the increase of Chlorophyll standards for fluorometer chlorophyll fluorescence (Merz et al., 1996) calibration were purchased from Turner . Designs, USA. PS II herbicides used were diuron (3-(3,4-dicholorophenyl)-1,1 Signal dimethylurea or DCMU), and propanil (3′,4′- PS II FSU herbicide dichloropropionanilide). Non PS II herbicides used as comparison were 2,4-D (2,4- Meter dichlorophenoxy)acetic acid) and Silvex Algal Chlorophyll Transducer (2,4,5-trichlorophenoxypropionic acid) (Aldrich Sigma). -
Genetic Engineering of Green Microalgae for the Production of Biofuel and High Value Products
GENETIC ENGINEERING OF GREEN MICROALGAE FOR THE PRODUCTION OF BIOFUEL AND HIGH VALUE PRODUCTS Joanna Beata Szaub Department of Structural and Molecular Biology University College London A thesis submitted for the degree of Doctor of Philosophy August 2012 DECLARATION I, Joanna Beata Szaub confirm that the work presented in this thesis is my own. Where information has been derived from other sources, I confirm that this has been indicated in the thesis. Signed: 1 ABSTRACT A major consideration in the exploitation of microalgae as biotechnology platforms is choosing robust, fast-growing strains that are amenable to genetic manipulation. The freshwater green alga Chlorella sorokiniana has been reported as one of the fastest growing and thermotolerant species, and studies in this thesis have confirmed strain UTEX1230 as the most productive strain of C. sorokiniana with doubling time under optimal growth conditions of less than three hours. Furthermore, the strain showed robust growth at elevated temperatures and salinities. In order to enhance the productivity of this strain, mutants with reduced biochemical and functional PSII antenna size were isolated. TAM4 was confirmed to have a truncated antenna and able to achieve higher cell density than WT, particularly in cultures under decreased irradiation. The possibility of genetic engineering this strain has been explored by developing molecular tools for both chloroplast and nuclear transformation. For chloroplast transformation, various regions of the organelle’s genome have been cloned and sequenced, and used in the construction of transformation vectors. However, no stable chloroplast transformant lines were obtained following microparticle bombardment. For nuclear transformation, cycloheximide-resistant mutants have been isolated and shown to possess specific missense mutations within the RPL41 gene. -
Lateral Gene Transfer of Anion-Conducting Channelrhodopsins Between Green Algae and Giant Viruses
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.15.042127; this version posted April 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 5 Lateral gene transfer of anion-conducting channelrhodopsins between green algae and giant viruses Andrey Rozenberg 1,5, Johannes Oppermann 2,5, Jonas Wietek 2,3, Rodrigo Gaston Fernandez Lahore 2, Ruth-Anne Sandaa 4, Gunnar Bratbak 4, Peter Hegemann 2,6, and Oded 10 Béjà 1,6 1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel. 2Institute for Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, Berlin 10115, Germany. 3Present address: Department of Neurobiology, Weizmann 15 Institute of Science, Rehovot 7610001, Israel. 4Department of Biological Sciences, University of Bergen, N-5020 Bergen, Norway. 5These authors contributed equally: Andrey Rozenberg, Johannes Oppermann. 6These authors jointly supervised this work: Peter Hegemann, Oded Béjà. e-mail: [email protected] ; [email protected] 20 ABSTRACT Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity 1,2. Four ChR families are currently known. Green algal 3–5 and cryptophyte 6 cation-conducting ChRs (CCRs), cryptophyte anion-conducting ChRs (ACRs) 7, and the MerMAID ChRs 8. Here we 25 report the discovery of a new family of phylogenetically distinct ChRs encoded by marine giant viruses and acquired from their unicellular green algal prasinophyte hosts. -
Taxonomy and Diversity of Genus Pediastrum Meyen (Chlorophyceae, Algae) in East Nepal
S.K. Rai and P.K.Our NatureMisra / (2012) Our Nature 10: 16 (2012)7-175 10: 167-175 Taxonomy and Diversity of Genus Pediastrum Meyen (Chlorophyceae, Algae) in East Nepal S.K. Rai1* and P.K. Misra2 1Department of Botany, Post Graduate Campus, T.U., Biratnagar, Nepal 2Phycology Research Laboratory, Department of Botany, University of Lucknow, India *E-mail: [email protected] Abstract Pediastrum Meyen is a green algae occurs frequently in lentic environment like pond, puddles, lakes etc. mostly in warm and humid terai region. Twenty taxa of Pediasturm have been reported from Nepal, mostly from central and western part of the country, hitherto. Among them, in the present study, ten taxa of Pediastrum are enumerated also from east Nepal. Taxonomy and diversity of each taxa have been described with photomicrography. Key words: Algae, Chlorophyceae, Pediastrum, Taxonomy, Nepal Introduction Green algae are aquatic plants and act as the combined with silicon oxide which makes pioneer photosynthetic organism or them high resistance to decay. Therefore, producer in the World of ecosystem. The they remain preserved well in lake genus Pediastrum Mayen (Chlorophyceae, sediments as fossil record for palynological Sphaeropleales) is a free floating, coenobial, studies (Komárek and Jankovská, 2001). green algae occurs commonly in natural Thus, the knowledge of Pediastrum can be freshwater lentic environments like ponds, useful for the determination of trophocity or lakes, reservoirs etc. Their occurrence in salinity of water at present and past brackish and salty waters is rare (Parra, (Pasztaleniec and Poniewozik, 2004). 1979). At present, only 24 species of Study on algal flora of Nepal is Pediastrum have been described from the incomplete and sporadic. -
The Draft Genome of Hariotina Reticulata (Sphaeropleales
Protist, Vol. 170, 125684, December 2019 http://www.elsevier.de/protis Published online date 19 October 2019 ORIGINAL PAPER Protist Genome Reports The Draft Genome of Hariotina reticulata (Sphaeropleales, Chlorophyta) Provides Insight into the Evolution of Scenedesmaceae a,b,2 c,d,2 b e f Yan Xu , Linzhou Li , Hongping Liang , Barbara Melkonian , Maike Lorenz , f g a,g e,1 a,g,1 Thomas Friedl , Morten Petersen , Huan Liu , Michael Melkonian , and Sibo Wang a BGI-Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China b BGI Education Center, University of Chinese Academy of Sciences, Beijing, China c China National GeneBank, BGI-Shenzhen, Jinsha Road, Shenzhen 518120, China d Department of Biotechnology and Biomedicine, Technical University of Denmark, Copenhagen, Denmark e University of Duisburg-Essen, Campus Essen, Faculty of Biology, Universitätsstr. 5, 45141 Essen, Germany f Department ‘Experimentelle Phykologie und Sammlung von Algenkulturen’ (EPSAG), University of Göttingen, Nikolausberger Weg 18, 37073 Göttingen, Germany g Department of Biology, University of Copenhagen, Copenhagen, Denmark Submitted October 9, 2019; Accepted October 13, 2019 Hariotina reticulata P. A. Dangeard 1889 (Sphaeropleales, Chlorophyta) is a common member of the summer phytoplankton of meso- to highly eutrophic water bodies with a worldwide distribution. Here, we report the draft whole-genome shotgun sequencing of H. reticulata strain SAG 8.81. The final assembly comprises 107,596,510 bp with over 15,219 scaffolds (>100 bp). This whole-genome project is publicly available in the CNSA (https://db.cngb.org/cnsa/) of CNGBdb under the accession number CNP0000705. © 2019 Elsevier GmbH. All rights reserved. Key words: Scenedesmaceae; genome; algae; comparative genomics. -
A Taxonomic Revision of Desmodesmus Serie Desmodesmus (Sphaeropleales, Scenedesmaceae)
Fottea, Olomouc, 17(2): 191–208, 2017 191 DOI: 10.5507/fot.2017.001 A taxonomic revision of Desmodesmus serie Desmodesmus (Sphaeropleales, Scenedesmaceae) Eberhard HEGEWALD1 & Anke BRABAND2 1 Grüner Weg 20, D–52382 Niederzier, Germany 2 LCG, Ostendstr. 25, D–12451 Berlin, Germany Abstract: The revision of the serie Desmodesmus, based on light microscopy, TEM, SEM and ITS2r DNA, allowed us to distinguish among the taxa Desmodesmus communis var. communis, var. polisicus, D. curvatocornis, D. rectangularis comb. nov., D. pseudocommunis n. sp. var. texanus n. var. and f. verrucosus n. f., D. protuberans, D. protuberans var. communoides var. nov., D. pseudoprotuberans n. sp., D. schmidtii n. sp. Keys were given for light microscopy, electron microscopy and ITS2r DNA. Key words: Desmodesmus, morphology, cell wall ultrastructure, cell size, ITS–2, new Desmodesmus taxa, phylogeny, taxonomy, variability INTRODUCTION E.HEGEWALD. Acutodesmus became recently a syno- nyme of Tetrades- mus (WYNNE & HALLAN 2016). Members of the former genus Scenedesmus s.l. were The subsection Desmodesmus as described by common in eutrophic waters all over the world. Hence HEGEWALD (1978) was best characterized by the cell taxa of that “genus” were described early in the 19th wall ultra–structure which consists of an outer cell wall century (e. g. TURPIN 1820; 1828; MEYEN 1828; EHREN- layer with net–like structure, lifted by tubes (PICKETT– BERG 1834; CORDA 1835). Several of the early (before HEAPS & STAEHELIN 1975; KOMÁREK & LUDVÍK1972; 1840) described taxa were insufficiently described and HEGEWALD 1978, 1997) and rosettes covered or sur- hence were often misinterpreted by later authors, es- rounded by tubes. -
Biodiversity of Microscopic Green Algae from Desert Soil Crusts
Digital Commons @ Assumption University Honors Theses Honors Program 2020 Biodiversity of Microscopic Green Algae from Desert Soil Crusts Victoria Williamson Assumption College Follow this and additional works at: https://digitalcommons.assumption.edu/honorstheses Part of the Life Sciences Commons Recommended Citation Williamson, Victoria, "Biodiversity of Microscopic Green Algae from Desert Soil Crusts" (2020). Honors Theses. 69. https://digitalcommons.assumption.edu/honorstheses/69 This Honors Thesis is brought to you for free and open access by the Honors Program at Digital Commons @ Assumption University. It has been accepted for inclusion in Honors Theses by an authorized administrator of Digital Commons @ Assumption University. For more information, please contact [email protected]. BIODIVERSITY OF MICROSCOPIC GREEN ALGAE FROM DESERT SOIL CRUSTS Victoria Williamson Faculty Supervisor: Karolina Fučíková Natural Science Department A Thesis Submitted to Fulfill the Requirements of the Honors Program at Assumption College Spring 2020 Williamson 1 Abstract In the desert ecosystem, the ground is covered with soil crusts. Several organisms exist here, such as cyanobacteria, lichens, mosses, fungi, bacteria, and green algae. This most superficial layer of the soil contains several primary producers of the food web in this ecosystem, which stabilize the soil, facilitate plant growth, protect from water and wind erosion, and provide water filtration and nitrogen fixation. Researching the biodiversity of green algae in the soil crusts can provide more context about the importance of the soil crusts. Little is known about the species of green algae that live there, and through DNA-based phylogeny and microscopy, more can be understood. In this study, DNA was extracted from algal cultures newly isolated from desert soil crusts in New Mexico and California. -
Distribution and Ecological Habitat of Scenedesmus and Related Genera in Some Freshwater Resources of Northern and North-Eastern Thailand
BIODIVERSITAS ISSN: 1412-033X Volume 18, Number 3, July 2017 E-ISSN: 2085-4722 Pages: 1092-1099 DOI: 10.13057/biodiv/d180329 Distribution and ecological habitat of Scenedesmus and related genera in some freshwater resources of Northern and North-Eastern Thailand KITTIYA PHINYO1,♥, JEERAPORN PEKKOH1,2, YUWADEE PEERAPORNPISAL1,♥♥ 1Department of Biology, Faculty of Science, Chiang Mai University. Chiang Mai 50200, Thailand. Tel. +66-53-94-3346, Fax. +66-53-89-2259, ♥email: [email protected], ♥♥ [email protected] 2Science and Technology Research Institute, Faculty of Science, Chiang Mai University. Chiang Mai 50200, Thailand Manuscript received: 12 April 2017. Revision accepted: 21 June 2017. Abstract. Phinyo K, Pekkoh J, Peerapornpisal Y. 2017. Distribution and ecological habitat of Scenedesmus and related genera in some freshwater resources of Northern and North-Eastern Thailand. Biodiversitas 18: 1092-1099. The family Scenedesmaceae is made up of freshwater green microalgae that are commonly found in bodies of freshwater, particularly in water of moderate to polluted water quality. However, as of yet, there have not been any studies on the diversity of this family in Thailand and in similar regions of tropical areas. Therefore, this research study aims to investigate the richness, distribution and ecological conditions of the species of the Scenedesmus and related genera through the assessment of water quality. The assessment of water quality was based on the physical and chemical parameters at 50 sampling sites. A total of 35 taxa were identified that were composed of six genera, i.e. Acutodesmus, Comasiella, Desmodesmus, Pectinodesmus, Scenedesmus and Verrucodesmus. Eleven taxa were newly recorded in Thailand. -
Efecto Del Consumo De Nitrógeno De La
Programa de Estudios de Posgrado EFECTO DEL CONSUMO DE NITRÓGENO DE LA MICROALGA Desmodesmus communis SOBRE LA COMPOSICIÓN BIOQUÍMICA, PRODUCTIVIDAD DE LA BIOMASA, COMUNIDAD BACTERIANA Y LONGITUD DE LOS TELÓMEROS TESIS Que para obtener el grado de EFECTO DEL CONSUMOMaestro DE NITROGENO en EN Ciencias LA MICROALGA Desmodesmus communis SOBRE LA COMPOSICIÓN BIOQUÍMICA, PRODUCTIVIDAD DE LA BIOMASA, COMUNIDAUso,D BACTERIANAManejo y Preservación Y LONGITUD deDE LOSlos RecursosTELÓMEROS Naturales (Orientación en Biotecnología) P r e s e n t a Jessica Guadalupe Elias Castelo La Paz, Baja California Sur, mayo 2018. CONFORMACIÓN DE COMITÉS Comité tutorial Co-Director de tesis Dra. Bertha Olivia Arredondo Vega - CIBNOR Co-Director de tesis Dr. Juan Pedro Luna Arias - CINVESTAV Tutor de tesis Dra. Thelma Rosa Castellanos Cervantes - CIBNOR Comité revisor Dra. Bertha Olivia Arredondo Vega Dr. Juan Pedro Luna Arias Dra. Thelma Rosa Castellanos Cervantes Jurado de examen Dra. Bertha Olivia Arredondo Vega Dr. Juan Pedro Luna Arias Dra. Thelma Rosa Castellanos Cervantes Suplente Dr. Dariel Tovar Ramírez i Resumen Las microalgas son organismos fotosintéticos que contienen clorofila y acumulan compuestos de interés biotecnológico. En condiciones de estrés como la disminución de la concentración de nitrógeno en el medio, incrementan la concentración de especies reactivas de oxígeno (ERO). Las ERO causan daño a biomoléculas como los lípidos, el ADN y sus telómeros, siendo estos responsables de la senescencia celular. La ausencia de daño estructural se debe a moléculas con capacidad antioxidante como los carotenoides. En el presente trabajo se evaluó el efecto del consumo de nitrógeno en el medio de cultivo y nitrógeno elemental interno así como sus razones isotópicas durante el crecimiento Desmodesmus communis sobre la tasa de crecimiento, productividad de la biomasa, la composición bioquímica, comunidad bacteriana y longitud de los telómeros. -
Table of Contents
Table of Contents General Program………………………………………….. 2 – 5 Poster Presentation Summary……………………………. 6 – 8 Abstracts (in order of presentation)………………………. 9 – 41 Brief Biography, Dr. Dennis Hanisak …………………… 42 1 General Program: 46th Northeast Algal Symposium Friday, April 20, 2007 5:00 – 7:00pm Registration Saturday, April 21, 2007 7:00 – 8:30am Continental Breakfast & Registration 8:30 – 8:45am Welcome and Opening Remarks – Morgan Vis SESSION 1 Student Presentations Moderator: Don Cheney 8:45 – 9:00am Wilce Award Candidate FUSION, DUPLICATION, AND DELETION: EVOLUTION OF EUGLENA GRACILIS LHC POLYPROTEIN-CODING GENES. Adam G. Koziol and Dion G. Durnford. (Abstract p. 9) 9:00 – 9:15am Wilce Award Candidate UTILIZING AN INTEGRATIVE TAXONOMIC APPROACH OF MOLECULAR AND MORPHOLOGICAL CHARACTERS TO DELIMIT SPECIES IN THE RED ALGAL FAMILY KALLYMENIACEAE (RHODOPHYTA). Bridgette Clarkston and Gary W. Saunders. (Abstract p. 9) 9:15 – 9:30am Wilce Award Candidate AFFINITIES OF SOME ANOMALOUS MEMBERS OF THE ACROCHAETIALES. Susan Clayden and Gary W. Saunders. (Abstract p. 10) 9:30 – 9:45am Wilce Award Candidate BARCODING BROWN ALGAE: HOW DNA BARCODING IS CHANGING OUR VIEW OF THE PHAEOPHYCEAE IN CANADA. Daniel McDevit and Gary W. Saunders. (Abstract p. 10) 9:45 – 10:00am Wilce Award Candidate CCMP622 UNID. SP.—A CHLORARACHNIOPHTYE ALGA WITH A ‘LARGE’ NUCLEOMORPH GENOME. Tia D. Silver and John M. Archibald. (Abstract p. 11) 10:00 – 10:15am Wilce Award Candidate PRELIMINARY INVESTIGATION OF THE NUCLEOMORPH GENOME OF THE SECONDARILY NON-PHOTOSYNTHETIC CRYPTOMONAD CRYPTOMONAS PARAMECIUM CCAP977/2A. Natalie Donaher, Christopher Lane and John Archibald. (Abstract p. 11) 10:15 – 10:45am Break 2 SESSION 2 Student Presentations Moderator: Hilary McManus 10:45 – 11:00am Wilce Award Candidate IMPACTS OF HABITAT-MODIFYING INVASIVE MACROALGAE ON EPIPHYTIC ALGAL COMMUNTIES. -
Evolutionary Relationships Between the Varieties of Green Algae Pediastrum Boryanum and P
170 Fottea, Olomouc, 18(2): 170–188, 2018 DOI: 10.5507/fot.2018.004 Evolutionary relationships between the varieties of green algae Pediastrum boryanum and P. duplex s.l. (Chlorophyceae, Hydrodictyaceae) Joanna Lenarczyk* & Marta Saługa W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 46, 31–512 Kraków, Poland; *Corresponding author e–mail: [email protected] Abstract: The infraspecific relationships of the two most variable species,Pediastrum boryanum and P. duplex s.l. identified on the basis of morphological criteria, have been poorly studied so far. The present study focused on 12 original strains isolated from Polish water bodies and 29 strains from the GenBank database, which represent P. boryanum, P. duplex s.l. varieties and other morphologically similar taxa, against the background of 14 other strains of the family Hydrodictyaceae. In order to estimate the level of congruence between the phylogeny and the classical taxonomic system based on morphological criteria, the strains of P. boryanum and P. duplex s.l., and other morphologically similar taxa were subjected to parallel phylogenetic analyses applying Bayesian and Maximum Likelihood methods to combined molecular data (26S rDNA and rbcL cpDNA) and morphological analyses based on light and scanning electron microscopy, and iconographic documentation of almost all strains published elsewhere. The gene topologies revealed many discrepancies in the morphological features used to delimit the analysed taxa. The polyphyly of both P. boryanum and P. duplex s.l. was confirmed. Pseudopediastrum boryanum var. cornutum (formerly P. boryanum var. cornutum) formed a well supported monophyletic clade, whereas other varieties, including var. boryanum, brevicorne and longicorne, proved to be complex taxa.