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Phytochemical Screening and Antimicrobial Activity of Carica Papaya L, Citrus Paradisi L, Citrus Sinensis L, and Vernonia Amygdalina Del

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Phytochemical Screening and Antimicrobial Activity of Carica Papaya L, Citrus Paradisi L, Citrus Sinensis L, and Vernonia Amygdalina Del Journal of Dairy & Veterinary Sciences ISSN: 2573-2196 Research Article Dairy and Vet Sci J Volume 9 Issue 4 - January 2019 Copyright © All rights are reserved by Nduche MU DOI: 10.19080/JDVS.2019.09.555768 Phytochemical Screening and Antimicrobial Activity of Carica Papaya L, Citrus Paradisi L, Citrus Sinensis L, and Vernonia Amygdalina Del. Cent Nduche MU*, Nkaa FA and Onyebinime A Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture, Nigeria Submission: January 12, 2019 ; Published: January 30, 2019 *Corresponding author: Nduche MU, Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture, Umudike PMB 7267 Umuahia Abia State, Nigeria Abstract The antimicrobial activity and phytochemical analysis were carried out on the leaves of Carica papaya L, Citrus paradise L Citrus sinensis L and Vernonia amygdalina. The aim of the work is to determine the phytochemical contents, diameter of inhibition and minimum inhibition proportions. The phytochemical contents of C. papaya (Pawpaw) were as follows: alkaloid (1.11 ± 0.02%), saponins (0.53 ± 0.04%), tannin (0.43 ±concentration of the grasses± 0.01%) studied. and The phenols four-plant (0.91 species ± 0.03%). were For found Citrus to Paradisi contain (Grape) alkaloid, alkaloid saponin, content tannins, was flavonoid (0.31 ± 0.05),and phenols saponins in varying(0.19 ± 0.01), tannins (0.25 ± ± 0.02), phenol (0.52 ± 0.04). In Citrus sinensis (Orange), alkaloid was (0.23 ± 0.01), saponin (0.23 ± 0.01), 0.4%), tannin flavonoid (0.15 ±(0.67 ± 0.01) and phenol (0.59 ± 0.07). For Vernonia amygdalina Del. Cent (Bitter leaf), alkaloid (1.31 ± 0.03), saponin (1.15 ± 0.01), flavonoid ±(0.36 0.02), tannin (0.43 ± 0.01), phenol (1.15 ± 0.01). 0.01), flavonoid (0.33 The anti-microbial 0.01), flavonoid activity of (0.71 the ethanolic and aqueous extracts of leaves of the plants were tested against four microorganisms; Salmonella pullorum, Klebsiella pneumonia, Enterobacter aerogenes and Proteus vulgaris. The susceptibility of the microorganisms was determined using the disk diffusion method. The leaf extract of these plants inhibited the growth of these pathogens. All the ethanolic leaf extracts of the plants inhibited the clinical isolates. The minimum inhibitory concentration was analyzed using agar well diffusion method with values ranging between 50 mg/ml and 200 mg/ml against all the clinical isolates. There were variations in the lowest concentrations of the different plant extracts that caused inhibitions against the various microorganisms. Keywords: Antimicrobial; Microorganisms; Medicinal; Phytochemicals; Nigeria Introduction [5]. In developing countries, the situation is even worse because Plants are rich in a wide variety of secondary metabolites with of poor sanitation and ignorance of good hygiene practice thus ex- posing many people to infectious agents. alkaloids [1]. The study of most research recently is to evaluate the anti-microbial properties such as flavonoid, tannins, phenols and anti-microbial activity of some local natural plants which have po- Phytochemicals are chemical compounds that occur naturally tential of treating infectious diseases and with reduced side effects in plants. They are chemicals produced by plants through prima- compared to the synthetic drug agents [2]. The use of plant ex- ry or secondary metabolism [6-8]. Some are responsible for col- tracts and phytochemicals, both with known antimicrobial prop- or and other organoleptic properties, such as the deep purple of blueberries and the smell of ginger. Plants are composed entirely Many plants have been used because of their anti-microbial prop- of chemicals of various kinds Phytochemical has bio-active consti- erties can be of great significance in the treatment of diseases. erties. This is mostly due to synthesis of secondary metabolism by the plants [3]. Discovery of new antimicrobial compounds with compounds [9]. The medicinal values of some plants lie in these diverse chemical structures and novel mechanism of action be- tutes such as alkaloids, tannis, flavonoids, saponins and phenolic comes of urgent attention. Currently, the development of resistant the human body. Many of these indigenous medicinal plants are chemical substances that produce definite physiological actions in strains of bacteria has increased the need for new antibiotics the used as spices and food plants [10]. Plant serves as rich resourc- antimicrobial compounds produced by plants are active against plants and human pathogenic microorganism [4]. Isolation of bac- medicinal effects of plant materials typically result from the com- es of natural drugs for research and development. The beneficial teria less susceptible to regular antibiotics and recovery of resis- binations of secondary products present in plants. The medicinal tant isolates during antibacterial therapy is now a global problem actions of plants are unique to plant species or groups as the com- Dairy and Vet Sci J 9(3): JDVS.MS.ID.555768 (2019) 001 Journal of Dairy & Veterinary Sciences bination of secondary products in a plant are often taxonomically of 2012, sweet orange accounted for approximately 70% of cit- distinct [11]. rus production. In 2014, 70.9million tones of orange were grown worldwide, with Brazil producing 24% of the world total followed The Aim and Objectives of this Work are by China and India. The orange peel is used as a dried seasoning, a. To determine the phytochemicals of Citris paradisi orange is effective in the management of asthma, arthritis, cholera (grape), Citrus sinensis C. (orange), Carica papaya (paw-paw) and etc. Citrus sinensis - Vernonia amygdalina (bitter leaf). (orange) peel extracts contain bioflavonoids in and hypolipodemic effects [16]. b. To determine antimicrobial activities of the aqueous and cluding poly methoxylated flavors, which have anti-inflammatory ethanolic leaf extracts of Carica papaya, Citris paradisi, Citrus sin- Vernonia amygdalina, a member of the Asteraceae family, is a ensis and Vernonia amygdalina, on Salmonella pullorum, Klebsiella shrub or small tree of 2-5m with petiolate leaf of about 6mm di- pneumonia, Enterobacter aerogenes and Proteus vulgaris ameter and elliptic shape. The leaves are green with a character- istic’s odor and a bitter taste. No seeds are produced and the tree The genus belongs to the family which is Carica Caricaceae has therefore to be distributed through cutting. It grows under a usually a single-stemmed, semi-woody giant herb with fast, inde- range of ecological zones in Africa, the leaves are washed before is Carica papaya eating to get rid of the bitter taste. They are used as vegetable and a sole species of the genus Carica. Caricaeceae, a family well rep- terminate growth;3m during the first year [12]. stimulate the digestive system, as well as to reduce fever. resented in the neotropics that includes six genera with at least 35 species It is spread to many tropical and subtropical regions They are used in medicine as anti-malarial, anti-microbial lax- around the world [13]. The plant produces large palmate leaves ative, anthelmintic, antithrombotic and both hypoglycemic and (0.6m2 hypohpidaemic effect in diabetics. It is also used in breast cancer 40 to 60cm the papaya plant develops very fast taking 3-8 months treatment Vernonia amygdalina can also be used alone or in com- ) with five to none pinnate lobes of various widths between bination with known drugs. Vernonia amygdalina extracts may Stem grows from 5 to 10m (16 to 33ft) tall, with spirally arranged help suppress, delay or kill cancerous cell in many ways, such as from seed germination to flowering and 9-15 months for harvest. - induction of apoptosis as determined in cell culture and animal uously scarred when the leaves and fruit were borne. The leaves studies [17]. Other medicinal importance includes, it helps in di- leaves confined to the top of the trunk. The lower trunk is conspic are large, 50 -70cm (20-28m) in diameter, deeply palmately lobed, gestion and reduce fever. Vernonia amygalina also enhances che- with seven lobes, all parts of the plant contain latex [14]. The ripe motherapy sensitivity, extract may render cancerous cells to be fruit of papaya is usually eaten raw without skin or seeds. The un- more sensitive to chemotherapy [18]. ripe green fruit can be eaten cooked, usually in curries, salads and stew. The leaves are used in herbal medicine to remove intestinal Materials and Methods worms Collection of Plant Materials and Identification Citrus paradise (grape) belong to the family Rutaceae, order The fresh and healthy leaves of the plant species - bitter leaf, Sapindales and species Citrus paradisi, it is a subtropical citrus paw-paw, grape, orange was obtained from different premise. Car- tree known for its sour to semi-sweet somewhat bitter fruit The ica papaya, Citrus paradise, Citrus sinensis and Vernonia amgydali- evergreen grape fruit trees usually grow to around 5-6 meters (16- na were obtained from the premises of Michael Okpara Universi- 20ft) tall, though they can reach 13-15m (43-49ft), the leaves are ty of Agriculture, Umudike, Abia State. Citrus paradisi was gotten glossy dark green, long (up to 15 centimeters (5.9inches) and thin. from the premises of National Root Crop Research Institute, Umu- The fruit is yellow-orange skinned and generally an oblate spher- - oid in shape, it ranges in diameter from 10-15cm (3.9 – 5.9inches). partment of Plant Science and Biotechnology, Michael Okpara Uni- dike Abia State. It was identified in the taxonomic unit of the De versity of Agriculture Umudike, Abia State. the cultivars which include, white, pink and red pulps of varying The flesh is segmented and acidic varying in color depending on sweetness. Grape has been investigated in cancer medicine phar- Preparation of the Sample for Analysis macodynamics and good source of vitamin C [15]. The extraction of the plant leaves was carried out using known The genus citrus belongs to the family Rutaceae.
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