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Wesley Frey Cv WESLEY FREY Curriculum Vitae Department of Biological Sciences 688 Life Sciences Building, Louisiana State University Ph. (225) 301-0512 Email: wfrey1@tigers.lsu.edu Summary Graduate Student, Lab of Dr. Joomyeong Kim (College of Science, Louisiana State University) B.S., College of Science, Louisiana State University Experienced in molecular biology techniques on a wide range of organisms Diverse teaching experience Professional Experience Teaching Experience Undergraduate Teaching Assistant (LSU) -- Summer 2008, Summer 2009 Under Advisement of: Tiffany A. Simms Introductory Biology Lab for non-majors (BIOL 1005) Introductory Biology Lab for majors (BIOL 1208) Graduate Teaching Assistant (LSU) -- December 2010-Present Employed By: Dr. Wischusen Introductory Biology Lab for non-majors (BIOL 1005) Introductory Biology Lab for majors (BIOL 1208) Principles of Genetics (BIOL 2153) Research Experience I am classically trained in molecular biology (DNA, RNA and protein work) in a wide array of organisms, including E. coli, C. reinhardtii, S. cerevisiae, M. musculus and standard cell culture lines (human and mouse). I have also been trained in mouse husbandry, including the maintenance of many mouse knockout lines as well as management of complex breeding schemes. Recent endeavors include mouse behavioral analysis (specifically involving maternal care) and “dry” bench work including bioinformatic analysis of ChIP-seq data and small RNA data with the use of custom shell scripting. Future interests are focused on expanding my toolbox in RNAi, from delivery of smRNAs in vivo, to large-scale analysis of target transcripts. In the long-run, I hope to develop an efficient ncRNA gene delivery system that is effective in long-term silencing of a specific gene of interest. Undergraduate Student Worker (LSU) – May 2007-December 2010 Advisor: Dr. James Moroney Research Focus: Carbonic Anhydrases, specifically CAH6, involvement in CCM of C. reinhardtii Graduate Student (LSU) – December 2010 - Present Advisor: Dr. Joomyeong Kim Research Focus: Molecular function and evolutionary conservation of APeg3 in M. musculus. Protein interactions and evolutionary conservation of Peg3. Behavioral and molecular characterization of Peg3 conditional knockout mice. Education B.S. Biochemistry (LSU) -- August 2006-December 2010, College of Science Minor: Chemistry Minor: Psychology PhD Candidate, Biological Sciences (LSU) -- August 2011-Present Advisor: Dr. Joomyeong Kim Publications Moroney J, Ma Y, Frey WD, Fusilier K, Pham T, Simms T, DiMario R, Yang J, Mukherjee B. The carbonic anhydrase isoforms of Chlamydomonas reinhardtii, intracellular location, expression and physiological roles. Photosynthesis Research (2011) 109: 133-149. Kim J, Ekram MB, Kim H, Faisal M, Frey WD, Huang JM, Tran K, Kim M, Yu S. Imprinting control region (ICR) of the Peg3 domain. Human Molecular Genetics (2012) 21 (12):2677-2687. Thiaville M, Kim H, Frey WD, Kim J. Identification of an Evolutionarily Conserved Cis-Regulatory Element Controlling the Peg3 Imprinted Domain. PLoS ONE (2013) 8(9): e75417. Kim J, Frey WD, He H, Kim H, Ekram MB, Bakshi A, Faisal M, Perera BPU, Ye A, Teruyama R. Peg3 mutational effects on reproduction and placenta-specific gene families. PLoS ONE (2013) 8(12): e83359. Frey WD and Kim J. APeg3: Regulation of Peg3 through an evolutionarily conserved ncRNA. Gene (2014): 540(2):251-7. Frey WD and Kim J. Tissue-specific Contributions of Peg3 in Neonate Growth, Maternal Care and Lactation (In Preparation) Honors and Awards Tuition Opportunity Program for Students scholarship (August 2006 – May 2010) LSU Board of Supervisor’s Scholarship (May 2007 – August 2010) Louisiana Pipeliner’s Association Scholarship (January 2008 – January 2011) BioGrads Symposium Poster Presentation (2014) – 1st Place Beta Beta Beta Honorary Society Panelist (Spring 2014) Published Abstracts Frey WD and Kim J. APeg3: Regulation of Peg3 through an evolutionarily conserved ncRNA. Gene March 8th, 2014. Keystone Symposia-Epigenetic Programming and Inheritance. Boston, Massachusetts. (Poster) Frey WD and Kim J. Peg3 Conditional Knockout: Tissue-specific Impacts on Milk Letdown and Maternal Care. November 21st, 2014. Louisiana State University BioGrads Symposium. – 1st Place Awarded - Baton Rouge, Louisiana. (Poster) References Dr. Joomyeong “Joo” Kim PhD Mentor Russell Thompson, Jr. Family Professor – Louisiana State University Contact: Phone: 225-578-7692 E-mail: jkim@lsu.edu Dr. James V. Moroney Undergraduate Mentor Streva Alumni Professor – Louisiana State University Contact: Phone: 225-578-8561 E-mail: btmoro@lsu.edu Dr. Mark Batzer Committee Member Boyd Professor – Louisiana State University Contact: Phone: 225-578-7102 E-mail: mbatzer@lsu.edu Dr. David Donze Committee Member – Life Mentor Associate Professor – Louisiana State University Contact: Phone: 225-578-7391 E-mail: ddonze@lsu.edu .
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