DOCSLIB.ORG

The Structure and Function of Grana-Free Thylakoid Membranes in Gerontoplasts of Senescent Leaves of Vicia Faba L

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Structure and Function of Grana-Free Thylakoid Membranes in Gerontoplasts of Senescent Leaves of Vicia Faba L The Structure and Function of Grana-Free Thylakoid Membranes in Gerontoplasts of Senescent Leaves of Vicia faba L. H. Oskar Schmidt Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, 4325 Gatersleben Z. Naturforsch. 43c, 149-154 (1988); received March 11/September 7, 1987 Senescence, Chloroplast, Gerontoplast, Thylakoid Membrane Proteins, Electronmicroscopy, Photosynthesis In the course of yellowing (senescence) the leaves of Vicia faba L. lose 95% of their chlorophyll. Gerontoplasts develop from chloroplasts and aggregate with the pycnotic mitochon- dria and the cell nucleus in the senescent cells (organelle aggregation). The gerontoplasts contain only a few, unstacked thylakoid membranes but a large number of carotinoid-containing plasto- globuli, which after the degration of chlorophyll presumably assume the light protection of the cells. The thylakoid membranes of the gerontoplasts were isolated by means of a flotation method. Their polypeptide composition is characterized by a high proportion of light-harvesting complex. Evidence of relatively high photochemical activity shows that functional thylakoid mem- branes are present in the premortal senescence state of leaves and this suggests that there is functional compartmentation of the hydrolytic processes in this stage of the leaves' development. Introduction sequently degradation of the thylakoid membranes During the senescence (yellowing) of leaves, and of chlorophyll commences. Lipids and Caroti- genetically programmed hydrolytic processes reg- noids released in the process collect, at least to some ulated by hormones take place. These lead to the extent, in globulare osmiophilic droplets designated degradation of the cell organelles and chloroplasts as plastoglobuli according to Lichtenthaler and Sprey and finally to lysis of the cells [1,2]. The leaf alters its [7], The "senescent chloroplast" containing irregu- most important function within the plant during this larly arranged grana stacks and numerous plasto- stage of development: the photosynthesizing ap- globuli, is formed; it usually also contains starch paratus is degraded; nitrogen compounds and carbo- grains and is typical of senescent leaves with its yel- hydrates are mobilized in the leaf and moved to the low-green colour. plant's storage organs or, in the case of annuals, to The further degradation of the thylakoid mem- the soil substrate. From being a C02 assimilation branes leads to a type of plastid that has only a few organ the leaf becomes a supplier of nutrients to the unstacked thylakoids, does not normally contain any plant. starch, and is filled mainly with plastoglobuli. This In spite of the progression of the degradation proc- premortal stage of chloroplast development was de- esses during leaf yellowing, the leaf cells, including scribed by Sitte [8, 31] as "gerontoplast". These or- the cell organelles and membranes in the cells at each ganelles contain only traces of chlorophyll and ac- particular stage of degradation, remain functional for cordingly their function also differs from that of the a prolonged period (up to several weeks). chloroplasts (see Discussion). Extensive biochemical investigations and examina- Numerous investigations have been carried out on tions by electron microscopy have been carried out grana-containing thylakoid membranes from mature on the degradation of chloroplasts, at least in the and senescent chloroplasts [9—12] but none are avail- initial stages of senescence [3-6]. As a rule, the able on thylakoids of gerontoplasts. In the present chloroplasts pass through a series of typical degrada- work these thylakoids were isolated by means of a tion stages. The content of ribulose diphosphate car- flotation method in order to examine the structure boxylase-oxygenase in the "mature" chloroplasts of and function of these final degradation products of green leaves drops with the onset of senescence. Sub- the thylakoid system of chloroplasts. Present address: Boschstraße, Postfach 220, D-6703 Lim- Materials and Methods burgerhof. Test object Reprint requests to Dr. O. Schmidt. Verlag der Zeitschrift für Naturforschung, D-7400 Tübingen Vicia faba L. cv. Dornburger Ackerbohne was 0341 -0382/88/159 - 0155 $01.30/0 grown for about 5 weeks in a hydroculture in a con- 150 H. O. Schmidt • Structure and Function of Grana-Free Thylakoid Membranes trolled environment chamber up to the 12-leaf stage. fone acid, respectively, as described by Metzger and The shoots of these plants were cut above and below Ohmann [14]. the 3rd leaf node and placed in bottles containing distilled water. In a controlled environment chamber Electrophoretic separation at 2000 lux and 18 °C the leaves on these shoot sec- The membranes were dissolved in 1% SDS, 1 mM tions turned yellow reproducibly within 7—9 days. phenylmethylsulfonyl fluoride, 1 mM iodoacetamide, 50 mM tris-HCl, pH 8.9, and the proteins were sepa- Electron microscopy rated in slab gels (10% polyacylamide and 5 M urea) as described by Schmidt [15]. Staining was carried Leaf sections and isolated thylakoid membranes out with Coomassie Brilliant blue G 250. Densito- were fixed with 3% glutaraldehyde and 1% 0s04, grams were prepared with the aid of a Zeiss Schnell- embedded in Epon and examined on a JEM 2 (Jeol, photometer III (VEB Carl Zeiss, Jena, GDR). Japan). Number of chloroplasts Results The determination of the chloroplast count per cell Yellowing of the leaves of Vicia faba / was carried out according to Possingham and Sauer The senescence of the field bean Vicia faba L. pro- [30]. ceeds progressively, i.e. the lower, oldest leaves begin to turn yellow on the flowering plant, while leaves and blossoms are being formed at the tip of Isolation of thylakoid membranes the shoot. In the case of plants growing outdoors, the The chloroplasts were isolated using a flotation weather causes most of the yellowing leaves to drop method [13]. Leaves that had turned completely yel- off before they have turned completely yellow and low (2 mg chlorophyll/cm2) were homogenized in iso- reached the final premortal senescent stage. lation buffer (75 MM tris-HCl, pH 7.8, 0.5 M sucrose, In order to obtain sufficient quantities of yellowed 0.2% human serum albumin, 0.2% polyvinylpyrroli- leaves for the isolation of gerontoplasts, green leaves done, 0.1 mM diisopropylfluorophosphate and were removed from the plant together with a piece of 0.1 mM iodoacetamide), pressed through nylon shoot and brought reproducibly to complete yellow- gauze and centrifuged for 2 min at 600 x g. The plas- ing in a controlled environment chamber. The leaves tids were separated from the supernatant by centrifu- used for isolating gerontoplasts were completely yel- gation (30 min at 20,000 xg), suspended in 50 mM low but were still turgescent (premortal state of tris-HCl, pH 7.5, 31% sucrose (w/w), 0.1 mM diiso- senescence). Under the chosen conditions they exhi- propylfluorophosphate and 0.1 mM iodoacetamide, bited the same features of senescence as leaves on and introduced into the middle layer of a multi-stage outdoor plants, both externally and under a light density gradient (17%, 21%, 25%, 31%, 33%, 35% microscope. During yellowing the chlorophyll con- and 42% sucrose (w/w), in 50 mM tris-HCl, pH 7.5). tent of the leaves dropped from 35 ± 2 (ig (S. E.) chl/ As the result of centrifugation (25 min at 40,000 x g), cm2 (=100%) to 1.5 ±0.4 [ig chl/cm2 (4.3%). In gerontoplasts floated to the surface of the gradient; addition, the ratio of chl a to chl b decreased from senescent chloroplasts were found in the 25% and 2.8 ±0.1 to 2.2 ±0.1, the protein content decreased 31% layers. Whole and fragmented mature chloro- from 382 ± 14 pg/cm2 to 88 ± 14 pg/cm2 (23.0%) and plasts sedimented into the 42% layer. The geronto- the nitrogen content from 165 ± 5 |ig/cm2 to plasts were collected and shocked osmotically with 45 ±2 jig/cm2 (27.3%). The number of chloroplasts distilled water. The thylakoids were then pelleted by per palisade cell remained constant: 98 ±5 were centrifugation (30 min at 40,000 Xg). counted in green leaves and 107 ± 7 in yellow leaves. Since no chloroplast division takes place during senescence, it may be concluded that there is no de- Assay of photosynthetic activity gradation of complete chloroplasts by phagocytosis The electron transport was measured from water, or lysis during this period and that thylakoid mem- benzidine and DCPIP/DCMU to anthraquinon sul- branes are always degraded inside the chloroplasts. 151 H. O. Schmidt • Structure and Function of Grana-Free Thylakoid Membranes Microscopic examination of chloroplasts and Under an electron microscope the chloroplast of gerontoplasts Vicia faba proved to be typical of higher plants Examination of green leaves under a light micro- (Fig. 2a). In the course of senescence the diameter scope showed that the chloroplasts in the cell were of the plastids decreased from 6 |xm to about 2 ^im: dispersed uniformly over the surface (Fig. la). They also moved into the part of the palisade cells Fig. 1. Electron micrographs of plastids in palisade cells from leaves of various stages of senescence, a) Green leaf (35 (xg chl/cm2); b) yellow-green leaf (7 (xg chl/cm2); c) yellow leaf (1.5 |xg chl/cm2). Magnification: x570. C = chloroplast. N = nucleus. Fig. 2. Electron micrographs of chloroplasts and gerontoplasts from leaves of Vicia faba. a) Chloroplast from a green leaf (35 ng chl/cm2) x 13,300; b) cell organelle aggregation: cell nucleus with gerontoplasts and pycnotic mitochondria from a yellow leaf (1.5 ng chl/cm2) x 7500; c) gerontoplast as b) x 20,000. 152 H. O. Schmidt • Structure and Function of Grana-Free Thylakoid Membranes away from the epidermis (Fig. lb), in which the cell nucleus was also located. In yellow leaves the plas- tids enclose the cell nucleus as a continuous casing (Fig.
Load more

Recommended publications
  • Synthetic Conversion of Leaf Chloroplasts Into Carotenoid-Rich Plastids Reveals Mechanistic Basis of Natural Chromoplast Development
    Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development Briardo Llorentea,b,c,1, Salvador Torres-Montillaa, Luca Morellia, Igor Florez-Sarasaa, José Tomás Matusa,d, Miguel Ezquerroa, Lucio D’Andreaa,e, Fakhreddine Houhouf, Eszter Majerf, Belén Picóg, Jaime Cebollag, Adrian Troncosoh, Alisdair R. Ferniee, José-Antonio Daròsf, and Manuel Rodriguez-Concepciona,f,1 aCentre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, 08193 Barcelona, Spain; bARC Center of Excellence in Synthetic Biology, Department of Molecular Sciences, Macquarie University, Sydney NSW 2109, Australia; cCSIRO Synthetic Biology Future Science Platform, Sydney NSW 2109, Australia; dInstitute for Integrative Systems Biology (I2SysBio), Universitat de Valencia-CSIC, 46908 Paterna, Valencia, Spain; eMax-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany; fInstituto de Biología Molecular y Celular de Plantas, CSIC-Universitat Politècnica de València, 46022 Valencia, Spain; gInstituto de Conservación y Mejora de la Agrodiversidad, Universitat Politècnica de València, 46022 Valencia, Spain; and hSorbonne Universités, Université de Technologie de Compiègne, Génie Enzymatique et Cellulaire, UMR-CNRS 7025, CS 60319, 60203 Compiègne Cedex, France Edited by Krishna K. Niyogi, University of California, Berkeley, CA, and approved July 29, 2020 (received for review March 9, 2020) Plastids, the defining organelles of plant cells, undergo physiological chromoplasts but into a completely different type of plastids and morphological changes to fulfill distinct biological functions. In named gerontoplasts (1, 2). particular, the differentiation of chloroplasts into chromoplasts The most prominent changes during chloroplast-to-chromo- results in an enhanced storage capacity for carotenoids with indus- plast differentiation are the reorganization of the internal plastid trial and nutritional value such as beta-carotene (provitamin A).
    [Show full text]
  • The Structure and Function of Plastids
    The Structure and Function of Plastids Edited by Robert R. Wise University of Wisconsin, Oshkosh WI, USA and J. Kenneth Hoober Arizona State University, Tempe AZ, USA Contents From the Series Editor v Contents xi Preface xix A Dedication to Pioneers of Research on Chloroplast Structure xxi Color Plates xxxiii Section I Plastid Origin and Development 1 The Diversity of Plastid Form and Function 3–25 Robert R. Wise Summary 3 I. Introduction 4 II. The Plastid Family 5 III. Chloroplasts and their Specializations 13 IV. Concluding Remarks 20 Acknowledgements 21 References 21 2 Chloroplast Development: Whence and Whither 27–51 J. Kenneth Hoober Summary 27 I. Introduction 28 II. Brief Review of Plastid Evolution 28 III. Development of the Chloroplast 32 IV. Overview of Photosynthesis 43 References 46 3 Protein Import Into Chloroplasts: Who, When, and How? 53–74 Ute C. Vothknecht and J¨urgen Soll Summary 53 I. Introduction 54 II. On the Road to the Chloroplast 56 III. Protein Translocation via Toc and Tic 58 IV. Variations on Toc and Tic Translocation 63 V. Protein Translocation and Chloroplast Biogenesis 64 VI. The Evolutionary Origin of Toc and Tic 66 VII. Intraplastidal Transport 66 VIII. Protein Translocation into Complex Plastids 69 References 70 xi 4 Origin and Evolution of Plastids: Genomic View on the Unification and Diversity of Plastids 75–102 Naoki Sato Summary 76 I. Introduction: Unification and Diversity 76 II. Endosymbiotic Origin of Plastids: The Major Unifying Principle 78 III. Origin and Evolution of Plastid Diversity 85 IV. Conclusion: Opposing Principles in the Evolution of Plastids 97 Acknowledgements 98 References 98 5 The Mechanism of Plastid Division: The Structure and Origin of The Plastid Division Apparatus 103–121 Shin-ya Miyagishima and Tsuneyoshi Kuroiwa Summary 104 I.
    [Show full text]
  • Molecular Plant Physiology
    2019 Molecular plant physiology LECTURE NOTES UNIVERSITY OF SZEGED www.u-szeged.hu EFOP-3.4.3-16-2016-00014 This teaching material has been made at the University of Szeged, and supported by the European Union. Project identity number: EFOP-3.4.3-16-2016-00014 MOLECULAR PLANT PHYSIOLOGY lecture notes Edited by: Prof. Dr. Attila Fehér Written by: Prof. Dr. Attila Fehér Dr. Jolán Csiszár Dr. Ágnes Szepesi Dr. Ágnes Gallé Dr. Attila Ördög © 2019 Prof. Dr. Attila Fehér, Dr. Jolán Csiszár, Dr. Ágnes Szepesi, Dr. Ágnes Gallé, Dr. Attila Ördög The text can be freely used for research and education purposes but its distribution in any form requires written approval by the authors. University of Szeged 13. Dugonics sq., 6720 Szeged www.u-szeged.hu www.szechenyi2020. Content Preface ..................................................................................................................................................... 5 Expected learning outputs ...................................................................................................................... 6 Chapter 1. The organization and expression of the plant genome ......................................................... 7 1.1. Organization of plant genomes .................................................................................................... 8 1.2.2. Structure of the chromatin .................................................................................................... 9 1.2. The plant genes .........................................................................................................................
    [Show full text]
  • Dynamic Metabolic Solutions to the Sessile Life Style of Plants Natural Product Reports
    Volume 35 Number 11 November 2018 Pages 1113–1230 Natural Product Reports rsc.li/npr Themed issue: Understanding Biosynthetic Protein-Protein Interactions – Part 2 Guest Editors: David Ackerley, Gregory Challis and Max Cryle ISSN 0265-0568 REVIEW ARTICLE Tomas Laursen et al. Dynamic metabolic solutions to the sessile life style of plants Natural Product Reports REVIEW View Article Online View Journal | View Issue Dynamic metabolic solutions to the sessile life style of plants Cite this: Nat. Prod. Rep.,2018,35, 1140 Camilla Knudsen, abc Nethaji Janeshawari Gallage, abc Cecilie Cetti Hansen, abc Birger Lindberg Møller abcd and Tomas Laursen *abc Covering: up to 2018 Plants are sessile organisms. To compensate for not being able to escape when challenged by unfavorable growth conditions, pests or herbivores, plants have perfected their metabolic plasticity by having developed the capacity for on demand synthesis of a plethora of phytochemicals to specifically respond to the challenges arising during plant ontogeny. Key steps in the biosynthesis of phytochemicals are catalyzed by membrane-bound cytochrome P450 enzymes which in plants constitute a superfamily. In planta, the P450s may be organized in dynamic fi Creative Commons Attribution-NonCommercial 3.0 Unported Licence. enzyme clusters (metabolons) and the genes encoding the P450s and other enzymes in a speci cpathway may be clustered. Metabolon formation facilitates transfer of substrates between sequential enzymes and therefore enables the plant to channel the flux of general metabolites towards biosynthesis of specific phytochemicals. In the plant cell, compartmentalization of the operation of specific biosynthetic pathways in specialized plastids serves to avoid undesired metabolic cross-talk and offersdistinctstoragesitesformolar concentrations of specific phytochemicals.
    [Show full text]
  • Identifying Regions of Conserved Synteny Between
    IDENTIFYING REGIONS OF CONSERVED SYNTENY BETWEEN PEA (PISUM SPP.), LENTIL (LENS SPP.), AND BEAN (PHASEOLUS SPP.) by Matthew Durwin Moffet A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Plant Sciences MONTANA STATE UNIVERSITY Bozeman, Montana August 2006 © COPYRIGHT by Matthew Durwin Moffet 2006 All Rights Reserved ii APPROVAL of a thesis submitted by Matthew Durwin Moffet This thesis has been read by each member of the thesis committee and has been found to be satisfactory regarding content, English usage, format, citations, bibliographic style, and consistency, and is ready for submission to the Division of Graduate Education. Dr. Norman Weeden Approved for the Department of Plant Sciences and Plant Pathology Dr. John Sherwood Approved for the Division of Graduate Education Dr. Carl A. Fox iii STATEMENT OF PERMISSION TO USE In presenting this thesis in partial fulfillment of the requirements for a master’s degree at Montana State University, I agree that the Library shall make it available to borrowers under rules of the Library. If I have indicated my intention to copyright this thesis by including a copyright notice page, copying is allowable only for scholarly purposes, consistent with “fair use” as prescribed in the U.S. Copyright Law. Requests for permission for extended quotation from or reproduction of this thesis in whole or in parts may be granted only by the copyright holder. Matthew Durwin Moffet August 2006 iv ACKNOWLEDGEMENTS I would like to acknowledge and thank the following individuals: my past and present academic advisors Dr. Dan Bergey, Dr.
    [Show full text]
  • Stromule Biogenesis in Maize Chloroplasts 192 6.1 Introduction 192
    Plastid Tubules in Higher Plants: An Analysis of Form and Function Mark T. Waters, BA (Oxon) A thesis submitted to the University of Nottingham for the degree of Doctor of Philosophy, September 2004 ABSTRACT Besides photosynthesis, plastids are responsible for starch storage, fatty acid biosynthe- sis and nitrate metabolism. Our understanding of plastids can be improved with obser- vation by microscopy, but this has been hampered by the invisibility of many plastid types. By targeting green fluorescent protein (GFP) to the plastid in transgenic plants, the visualisation of plastids has become routinely possible. Using GFP, motile, tubular protrusions can be observed to emanate from the plastid envelope into the surrounding cytoplasm. These structures, called stromules, vary considerably in frequency and length between different plastid types, but their function is poorly understood. During tomato fruit ripening, chloroplasts in the pericarp cells differentiate into chro- moplasts. As chlorophyll degrades and carotenoids accumulate, plastid and stromule morphology change dramatically. Stromules become significantly more abundant upon chromoplast differentiation, but only in one cell type where plastids are large and sparsely distributed within the cell. Ectopic chloroplast components inhibit stromule formation, whereas preventing chloroplast development leads to increased numbers of stromules. Together, these findings imply that stromule function is closely related to the differentiation status, and thus role, of the plastid in question. In tobacco seedlings, stromules in hypocotyl epidermal cells become longer as plastids become more widely distributed within the cell, implying a plastid density-dependent regulation of stromules. Co-expression of fluorescent proteins targeted to plastids, mi- tochondria and peroxisomes revealed a close spatio-temporal relationship between stromules and other organelles.
    [Show full text]
  • Plastoglobuli
    PP68CH10-Kessler ARI 6 April 2017 9:9 Plastoglobuli: Plastid Microcompartments with ANNUAL REVIEWS Further Click here to view this article's online features: Integrated Functions in • Download figures as PPT slides • Navigate linked references • Download citations • Explore related articles Metabolism, Plastid • Search keywords Developmental Transitions, and Environmental Adaptation Klaas J. van Wijk1 and Felix Kessler2 1Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York 14853; email: [email protected] 2Laboratory of Plant Physiology, University of Neuchatel,ˆ 2000 Neuchatel,ˆ Switzerland; email: [email protected] Annu. Rev. Plant Biol. 2017. 68:253–89 Keywords First published online as a Review in Advance on lipoprotein particle, membrane lipid monolayer, prenyl lipids, tocopherol, January 25, 2017 quinones, ABC1 kinases, chromoplast, elaioplast, leucoplast, gerontoplast, The Annual Review of Plant Biology is online at chloroplast, thylakoid plant.annualreviews.org https://doi.org/10.1146/annurev-arplant-043015- Abstract 111737 Plastoglobuli (PGs) are plastid lipoprotein particles surrounded by a mem- Copyright c 2017 by Annual Reviews. brane lipid monolayer. PGs contain small specialized proteomes and All rights reserved metabolomes. They are present in different plastid types (e.g., chloroplasts, chromoplasts, and elaioplasts) and are dynamic in size and shape in re- sponse to abiotic stress or developmental transitions. PGs in chromoplasts are highly enriched in carotenoid esters and enzymes involved in carotenoid metabolism. PGs in chloroplasts are associated with thylakoids and contain ∼30 core proteins (including six ABC1 kinases) as well as additional pro- Annu. Rev. Plant Biol. 2017.68:253-289. Downloaded from www.annualreviews.org teins recruited under specific conditions.
    [Show full text]
  • Different Genetic and Molecular Biology Approaches Used To
    MOLECULAR AND GENETIC STUDIES OF IRON HOMEOSTASIS IN ARABIDOPSIS ___________________________________________ A Dissertation Presented to the Faculty of the Graduate School University of Missouri-Columbia ___________________________________________ In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy ___________________________________________ by ALBERTO MAURER Dr. Elizabeth E. Rogers Dissertation Supervisor MAY 2006 © Copyright by All Rights Reserved The undersigned, appointed by the Dean of the Graduate School, have examined the dissertation entitled MOLECULAR AND GENETIC STUDIES OF IRON HOMEOSTASIS IN ARABIDOPSIS Presented by ALBERTO MAURER A candidate for the degree of Doctor of Philosophy And hereby certify that in their opinion it is worthy of acceptance ______________________________ Dr. Elizabeth E. Rogers ______________________________ Dr. Michael Petris ______________________________ Dr. John C. Walker ______________________________ Dr. Walter Gassmann ______________________________ Dr. Joseph C. Polacco ACKNOWLEDGEMENTS First of all, I would like to thank my advisor, Dr. Elizabeth E. Rogers, for giving me the opportunity to complete my Ph.D. degree in her lab. I was only able to finish this thesis thanks to her patience and guidance. Second, I would like to thank all the people and organizations that were able to support my studies in the form of assistantships: The Genetics Area Program and its former director Dr. Donald Riddle for my first-year assistantship; Dr. Douglass Randall and the Interdisciplinary Plant Group for the support given to me in the form of a 3-year assistantship; Dr. Elizabeth E. Rogers who supported my studies for 2 years; and to the Monsanto Corporation, who awarded me a senior grad-student assistantship. Thanks to all of you I was able to complete my degree.
    [Show full text]
  • The Marine Diatom Phaeodactylum Tricornutum and the Green Algae Chlamydomonas Reinhardtii Serena Flori
    Light utilization in microalgae : the marine diatom Phaeodactylum tricornutum and the green algae Chlamydomonas reinhardtii Serena Flori To cite this version: Serena Flori. Light utilization in microalgae : the marine diatom Phaeodactylum tricornutum and the green algae Chlamydomonas reinhardtii. Agricultural sciences. Université Grenoble Alpes, 2016. English. NNT : 2016GREAV080. tel-01686353 HAL Id: tel-01686353 https://tel.archives-ouvertes.fr/tel-01686353 Submitted on 17 Jan 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THÈSE Pour obtenir le grade de DOCTEUR DE LA COMMUNAUTE UNIVERSITE GRENOBLE ALPES Spécialité : Biologie Végétale Arrêté ministériel : 7 août 2006 Présentée par Serena FLORI Thèse dirigée par Giovanni FINAZZI et codirigée par Dimitris PETROUTSOS préparée au sein du Laboratoire de Physiologie Cellulaire et Végétale dans l'École Doctorale Chimie et Science du Vivant Light utilization in microalgae: the marine diatom Phaeodactylum tricornutum and the green algae Chlamydomonas reinhardtii. Thèse soutenue publiquement le 15 Septembre
    [Show full text]
  • WO 2017/079676 Al O
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/079676 Al 11 May 2017 (11.05.2017) W P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every B82Y 15/00 (201 1.01) A O1H 3/00 (2006.01) kind of national protection available): AE, AG, AL, AM, G01N 21/76 (2006.01) A01H 5/00 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/US20 16/060704 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 4 November 2016 (04.1 1.2016) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (25) Filing Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/25 1,071 4 November 2015 (04. 11.2015) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: MASSACHUSETTS INSTITUTE OF GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TECHNOLOGY [US/US]; 77 Massachusetts Avenue, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Cambridge, MA 02139-4307 (US).
    [Show full text]
  • Unraveling the ORE1 Regulon in Arabidopsis Thaliana : Molecular
    Max Planck-Institut für Molekulare Pflanzenbiologie und Universität Potsdam Arbeitsgruppe Prof. Dr. Bernd Mueller-Roeber Unraveling the ORE1 Regulon in Arabidopsis thaliana: Molecular and Functional Characterization of Up- and Down-stream Components Dissertation zur Erlangung des akademischen Grades “doctor rerum naturalium” (Dr. rer. nat.) in der Wissenschaftsdisziplin “Molekularbiologie” eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Potsdam von Lilian Paola Matallana-Ramírez Potsdam, den 11.04.2012 Published online at the Institutional Repository of the University of Potsdam: URL http://opus.kobv.de/ubp/volltexte/2012/6264/ URN urn:nbn:de:kobv:517-opus-62646 http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-62646 Lilian Paola Matallana-Ramírez Dedicated to my beloved deceased grandma Graciela “-Try not to become a man of success but rather to become a man of value”. A. Einstein ii Contents Summary Leaf senescence is an active process required for plant survival, and it is flexibly controlled, allowing plant adaptation to environmental conditions. Although senescence is largely an age-dependent process, it can be triggered by environmental signals and stresses. Leaf senescence coordinates the breakdown and turnover of many cellular components, allowing a massive remobilization and recycling of nutrients from senescing tissues to other organs (e.g., young leaves, roots, and seeds), thus enhancing the fitness of the plant. Such metabolic coordination requires a tight regulation of gene expression. One important mechanism for the regulation of gene expression is at the transcriptional level via transcription factors (TFs). The NAC TF family (NAM, ATAF, CUC) includes various members that show elevated expression during senescence, including ORE1 (ANAC092/AtNAC2) among others.
    [Show full text]
  • Chloroplast Biogenesis During Rehydration of the Resurrection Plant Xerophyta Humilis: Parallels to the Etioplast–Chloroplast Transition
    Plant, Cell and Environment (2008) 31, 1813–1824 doi: 10.1111/j.1365-3040.2008.01887.x Chloroplast biogenesis during rehydration of the resurrection plant Xerophyta humilis: parallels to the etioplast–chloroplast transition ROBERT A. INGLE1*, HELEN COLLETT1*, KEREN COOPER1, YUICHIRO TAKAHASHI2, JILL M. FARRANT1 & NICOLA ILLING1 1Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa and 2The Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan •- ABSTRACT initially the superoxide radical (O2 ) and singlet oxygen 1 ( O2) (Ivanov & Khorobrykh 2003; Møller, Jensen & De-etiolation of dark-grown seedlings is a commonly used Hansson 2007), and chloroplasts contain several antioxi- experimental system to study the mechanisms of chloro- dant systems to scavenge ROS (Apel & Hirt 2004; Foyer & plast biogenesis, including the stacking of thylakoid mem- Noctor 2005). The equilibrium between ROS production branes into grana, the response of the nuclear-chloroplast and scavenging can be perturbed by environmental stresses transcriptome to light, and the ordered synthesis and leading to a rapid increase in ROS concentration (Apel & assembly of photosystem II (PSII). Here, we present the Hirt 2004; Møller et al. 2007). Under water-deficit stress, xeroplast to chloroplast transition during rehydration of the especially under high-light conditions, the excitation energy Xerophyta humilis resurrection plant as a novel system for harvested by chlorophyll can greatly exceed the demand of studying chloroplast biogenesis, and investigate the role of the Calvin cycle for ATP and NADPH, leading to overre- light in this process. Xeroplasts are characterized by the duction of the electron transport chain and enhanced gen- presence of numerous large and small membrane-bound eration of ROS (Smirnoff 1993; Apel & Hirt 2004; Møller vesicles and the complete absence of thylakoid membranes.
    [Show full text]