Catalog # Aliquot Size

F12-911-05 3 x 5 nmol F12-911-20 3 x 20 nmol F12-911-50 3 x 50 nmol

FLT3 siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # F12-911

Lot # Z2037-48

Specificity Formulation FLT3 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human FLT3 expression. shipped at room temperature.

Product Description Reconstitution Protocol FLT3 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human FLT3 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_004119. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot FLK2, STK1, CD135 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or FGFR1 (FLT2), Active F04-11G below -20oC. After resuspension, siRNA stock solutions ≥2 FLT1, Active F11-11G µM can undergo up to 50 freeze-thaw cycles without FLT3 Mutant (D835Y), Active F12-12BG significant degradation. For long-term storage, it is FLT3, Active F12-11G recommended that the siRNA is stored at -70oC. For most FLT4, Active F13-11G favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background FLT3 is a that has been shown to play a role in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B FLT3 siRNA Set I siRNA duplexes targeted against three exon regions lymphoid progenitors (1). FLT3 consists of an extracellular domain composed of five immunoglobulin-like domains, Catalog Number F12-911 one transmembrane region, and a cytoplasmic kinase Specific Lot Number Z2037-48 domain split into two parts by a kinase-insert domain. FLT3 Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol is the most frequently mutated gene in cases of acute Format Lyophilized powder myelogenous leukemia (AML). About 30 to 35% of Stability 1yr at -70oC from date of shipment patients have either internal tandem duplications (ITDs) in Storage & Shipping The lyophilized powder is stable for at the juxtamembrane domain or mutations in the least 4 weeks at room temperature. It is recommended that the lyophilized activating loop of FLT3 (2). The consequence of either and resuspended siRNAs are stored at FLT3-ITD or activating loop mutations is the constitutive or below -20oC. After resuspension, activation of the tyrosine kinase activity. siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles without significant degradation. For References long-term storage, it is recommended 1. Christensen, J L. et al : Flk-2 is a marker in that the siRNA is stored at -70oC. For most favorable performance, avoid hematopoietic stem cell differentiation: a simple repeated handling and multiple method to isolate long-term stem cells. Proc. Nat. freeze/thaw cycles. Acad. Sci. 98: 14541-14546, 2001. 2. Gilliland, D G. et al: Role of FLT3 in leukemia. Curr Opin Hematol. 2002 Jul;9(4):274-81.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.