bioRxiv preprint doi: https://doi.org/10.1101/712000; this version posted May 16, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Characterization of Rose rosette virus and development of reverse genetic system for studying 2 virus accumulation and movement in whole plants. 3 Jeanmarie Verchotab1, Venura Herathab, Cesar D. Urrutiab, Mathieu Gayrala, Kelsey Lylead, 4 Madalyn K. Shiresb, Kevin Ongb, David Byrned, 5 aTexas A&M Agrilife Center in Dallas, Dallas Texas, USA 6 bDepartment of Plant Pathology & Microbiology, Texas A&M University, College Station, Texas, 7 USA 8 cDepartment of Horticultural Sciences, Texas A&M University, College Station, Texas, USA 9 dDepartment of Biological Sciences, University of Texas at Dallas, Dallas, Texas, USA 10 11 Running Head: Advancing Rose Rosette virus infectious clone 12 1 Address correspondence to Jeanmarie Verchot,
[email protected] 13 Present Address: Department of Plant Pathology & Microbiology, Texas A&M University, 14 College Station, Texas, USA 15 Word count for Abstract: 247 For text: 16 Keywords: Reverse genetic system, Emaravirus, plant virus, infectious clone, 17 18 19 20 21 1 bioRxiv preprint doi: https://doi.org/10.1101/712000; this version posted May 16, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 ABSTRACT 2 Rose rosette virus (RRV) is an Emaravirus, a negative-sense RNA virus with a 7-segmented 3 genome that is enclosed by a double membrane.