The Ethical Implications of Human Cloning
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Welfare Issues with Genetic Engineering and Cloning of Farm Animals
WellBeing International WBI Studies Repository 2006 Welfare Issues with Genetic Engineering and Cloning of Farm Animals The Humane Society of the United States Follow this and additional works at: https://www.wellbeingintlstudiesrepository.org/ hsus_reps_impacts_on_animals Part of the Animal Studies Commons, Bioethics and Medical Ethics Commons, and the Other Genetics and Genomics Commons Recommended Citation The Humane Society of the United States, "Welfare Issues with Genetic Engineering and Cloning of Farm Animals" (2006). IMPACTS ON FARM ANIMALS. 21. https://www.wellbeingintlstudiesrepository.org/hsus_reps_impacts_on_animals/21 This material is brought to you for free and open access by WellBeing International. It has been accepted for inclusion by an authorized administrator of the WBI Studies Repository. For more information, please contact [email protected]. Farm Animal Welfare • The HSUS 2100 L St., N.W. • Washington, DC 20037 • HSUS.org 202-452-1100 • F: 301-258-3081 • FarmAnimalWelfare.org An HSUS Report: Welfare Issues with Genetic Engineering and Cloning of Farm Animals Abstract Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. These animals suffer from unusually high rates of birth defects, disabili- ties, and premature death. Genetically engineering farm animals for greater bone strength or reduced pain reception, for example, may result in improved well-being, yet the broad use of this technology often causes increased suffering. The limited success of gene insertion techniques can result in genes failing to reach target cells and finishing in cells of unintended organs, and abnormally developed embryos may die in utero, be infertile, or born with development defects, attributable in part to insertional problems. -
Human Cloning: Must We Sacrifice Medical Research in the Name of a Total Ban?
S. HRG. 107–812 HUMAN CLONING: MUST WE SACRIFICE MEDICAL RESEARCH IN THE NAME OF A TOTAL BAN? HEARING BEFORE THE COMMITTEE ON THE JUDICIARY UNITED STATES SENATE ONE HUNDRED SEVENTH CONGRESS SECOND SESSION FEBRUARY 5, 2002 Serial No. J–107–55 Printed for the use of the Committee on the Judiciary ( U.S. GOVERNMENT PRINTING OFFICE 83–684 PDF WASHINGTON : 2002 For sale by the Superintendent of Documents, U.S. Government Printing Office Internet: bookstore.gpo.gov Phone: toll free (866) 512–1800; DC area (202) 512–1800 Fax: (202) 512–2250 Mail: Stop SSOP, Washington, DC 20402–0001 VerDate Feb 1 2002 09:13 Jan 16, 2003 Jkt 083684 PO 00000 Frm 00001 Fmt 5011 Sfmt 5011 C:\HEARINGS\83684.TXT SJUD4 PsN: CMORC COMMITTEE ON THE JUDICIARY PATRICK J. LEAHY, Vermont, Chairman EDWARD M. KENNEDY, Massachusetts ORRIN G. HATCH, Utah JOSEPH R. BIDEN, JR., Delaware STROM THURMOND, South Carolina HERBERT KOHL, Wisconsin CHARLES E. GRASSLEY, Iowa DIANNE FEINSTEIN, California ARLEN SPECTER, Pennsylvania RUSSELL D. FEINGOLD, Wisconsin JON KYL, Arizona CHARLES E. SCHUMER, New York MIKE DEWINE, Ohio RICHARD J. DURBIN, Illinois JEFF SESSIONS, Alabama MARIA CANTWELL, Washington SAM BROWNBACK, Kansas JOHN EDWARDS, North Carolina MITCH MCCONNELL, Kentucky BRUCE A. COHEN, Majority Chief Counsel and Staff Director SHARON PROST, Minority Chief Counsel MAKAN DELRAHIM, Minority Staff Director (II) VerDate Feb 1 2002 09:13 Jan 16, 2003 Jkt 083684 PO 00000 Frm 00002 Fmt 5904 Sfmt 5904 C:\HEARINGS\83684.TXT SJUD4 PsN: CMORC C O N T E N T S STATEMENTS OF COMMITTEE MEMBERS Page Brownback, Hon. -
Cloning: Adult Vs. Embryonic Cells and Techniques Employed
University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Supervised Undergraduate Student Research Chancellor’s Honors Program Projects and Creative Work Spring 5-2001 Cloning: Adult vs. Embryonic Cells and Techniques Employed Rebecca Ruth Frazor University of Tennessee-Knoxville Follow this and additional works at: https://trace.tennessee.edu/utk_chanhonoproj Recommended Citation Frazor, Rebecca Ruth, "Cloning: Adult vs. Embryonic Cells and Techniques Employed" (2001). Chancellor’s Honors Program Projects. https://trace.tennessee.edu/utk_chanhonoproj/459 This is brought to you for free and open access by the Supervised Undergraduate Student Research and Creative Work at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Chancellor’s Honors Program Projects by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. UNIVERSITY HONORS PROGRAM SENIOR PROJECT - APPROVAL Name: Getec eCA f?- f(CAZOr College: AQn eMi:hA rv Department: A1 (met I SCi!A1w -J Faculty Mentor: F. N-S ChV\C ¥: PROJECT TITLE: C10l/1\(}q : Adult \[) ' fmmrVVl l0 C l, (( ~ ~rd the. ~ Qr\L\A<; Icchn \ quc ~ Emplo\( f d I have reviewed this completed senior honors thesis with this student and certify that it is a project commens rate with rs level undergraduate research in this field. Signed: ;. ./-1 Faculty Mentor Date: _-+-'""-+----L-___ Comments (Optional): Cloning: Adult vs. Embryonic Cells and Various Techniques Employed Rebecca R. Frazor Mentor: F. Neal Schrick Senior Honors Project May 2001 Abstract The cloning of amphibians and mainly mammals is discussed focusing on somatic cell usage. Significant progress has been made in recent years and this is outlined with an emphasis on techniques and their differences. -
Hindu Bioethics for the Twenty-First Century
Journal of Hindu-Christian Studies Volume 14 Article 9 January 2001 Hindu Bioethics for the Twenty-First Century S. Cromwell Crawford Follow this and additional works at: https://digitalcommons.butler.edu/jhcs Part of the Religion Commons Recommended Citation Crawford, S. Cromwell (2001) "Hindu Bioethics for the Twenty-First Century," Journal of Hindu-Christian Studies: Vol. 14, Article 9. Available at: https://doi.org/10.7825/2164-6279.1252 The Journal of Hindu-Christian Studies is a publication of the Society for Hindu-Christian Studies. The digital version is made available by Digital Commons @ Butler University. For questions about the Journal or the Society, please contact [email protected]. For more information about Digital Commons @ Butler University, please contact [email protected]. -- Crawford: Hindu Bioethics for the Twenty-First Century Hindu Bioethics for the Twenty-First Century s. Cromwell Crawford University of Hawaii Introduction focus on these three distinctive features of Hindu bioethics, namely: its medical basis; IN his Cross Cultural Perspectives in its philosophical framework; its ethical Medical Ethics: Readings, Robert Veatch orientation. observes, "The religions of Judaism and Christianity and the secular thought of the Distinctive Features of Hindu Bioethics political philosophy of liberalism in the Anglo-American West are not the only Medical Basis alternatives to a Hippocratic medical ethic."J A unique feature of Hinduism is that a fully In fact, the new pluralistic approach to world fledged system of medicine evolved within .cultures is introducing us to several religious its complex ethos. The historical and philosophical alternatives from outside developments are shrouded in mystery due the Anglo-American West. -
Direct Cloning and Refactoring of a Silent Lipopeptide Biosynthetic Gene Cluster Yields the Antibiotic Taromycin A
Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A Kazuya Yamanakaa,b, Kirk A. Reynoldsa,c, Roland D. Kerstena, Katherine S. Ryana,d, David J. Gonzaleze, Victor Nizete,f, Pieter C. Dorresteina,c,f, and Bradley S. Moorea,f,1 aScripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093; bYokohama Research Center, JNC Corporation, Yokohama 236-8605, Japan; cDepartment of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093; dDepartment of Chemistry, University of British Columbia, Vancouver, BC, Canada V62 1Z4; and Departments of ePediatrics and fSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093 Edited by Jerrold Meinwald, Cornell University, Ithaca, NY, and approved December 23, 2013 (received for review October 23, 2013) Recent developments in next-generation sequencing technologies genes (9, 10). Synthetic biology approaches also can be used to have brought recognition of microbial genomes as a rich resource refactor orphan gene clusters by optimizing promoters, tran- for novel natural product discovery. However, owing to the scar- scriptional regulation, ribosome-binding sites, and even codon city of efficient procedures to connect genes to molecules, only use (11). Examples of successful regulatory gene manipulation to a small fraction of secondary metabolomes have been investigated elicit the production of new natural products from silent bio- to date. Transformation-associated recombination (TAR) cloning synthetic gene clusters in WT strains have been reported in takes advantage of the natural in vivo homologous recombination bacteria and fungi (12, 13); however, this approach requires the of Saccharomyces cerevisiae to directly capture large genomic loci. -
Dolly the Sheep – the First Cloned Adult Animal
DOLLY THE SHEEP – THE FIRST CLONED ADULT ANIMAL NEW TECHNOLOGY FOR IMPROVING LIVESTOCK From Squidonius via Wikimedia Commons In 1996, University of Edinburgh scientists celebrated the birth of Dolly the Sheep, the first mammal to be cloned using SCNT cloning is the only technology adult somatic cells. The Edinburgh team’s success followed available that enables generation of 99.8% its improvements to the single cell nuclear transfer (SCNT) genetically identical offspring from selected technique used in the cloning process. individuals of adult animals (including sterilized animals). As such, it is being Dolly became a scientific icon recognised worldwide and exploited as an efficient multiplication tool SCNT technology has spread around the world and has been to support specific breeding strategies of used to clone multiple farm animals. farm animals with exceptionally high genetic The cloning of livestock enables growing large quantities of value. the most productive, disease resistant animals, thus providing more food and other animal products. Sir Ian Wilmut (Inaugural Director of MRC Centre for Regeneration and Professor at CMVM, UoE) and colleagues worked on methods to create genetically improved livestock by manipulation of stem cells using nuclear transfer. Their research optimised interactions between the donor nucleus and the recipient cytoplasm at the time of fusion and during the first cell cycle. Nuclear donor cells were held in mitosis before being released and used as they were expected to be passing through G1 phase. CLONING IN COMMERCE, CONSERVATION OF AGRICULTURE AND PRESERVATION ANIMAL BREEDS OF LIVESTOCK DIVERSITY Cloning has been used to conserve several animal breeds in the recent past. -
How They Cloned a Sheep
How They Cloned A Sheep How They Cloned A Sheep This text is provided courtesy of the American Museum of Natural History. How they cloned a sheep 1. Scientists took udder cells from Dolly's DNA mother. They let the cells multiply and then they stopped the process when they had divided enough. 2. They took an egg cell from a different sheep and removed the nucleus. 3. They put one udder cell next to the egg cell without a nucleus and joined them using electricity. The egg cell now contained all the udder cell's DNA. 4.The egg cell divided until it developed into an embryo. An embryo is the early stage of an animal before it has been born or hatched. This embryo was placed inside a third sheep. Five months later, this sheep gave birth to Dolly. ReadWorks.org · © 2015 ReadWorks®, Inc. All rights reserved. How They Cloned A Sheep - Comprehension Questions Answer Key 1. What did scientists remove from the egg cell of a sheep? A. the nucleus B. the embryo C. DNA D. udder cells 2. This passage describes the sequence of events involved in cloning a sheep. What happened after the scientists took udder cells and an egg cell from different sheep? A. The egg cell was joined to an udder cell using electricity. B. The embryo was placed inside another sheep. C. Scientists took udder cells from Dolly's DNA mother. D. The egg cell divided until it formed an embryo. 3. As part of the process of cloning a sheep, scientists placed the embryo inside a sheep, which eventually gave birth to Dolly. -
Human Cloning: Fact Or Fiction
LRN: 05D1721 745. 6239 - i/3 Human Cloning: Fact or Fiction An Interactive Qualifying Project Report submitted to the Faculty of the Worcester Polytechnic Institute in partial fulfillment of the requirements for a Bachelor's Degree by Krisha S. Murthy Christopher Tereshko Daniel J. Caloia Amy Borges Date: May 3, 2005 Professor Thomas A. Shannon, Major Advisor 1 Abstract Human Cloning: Fact or Fiction is an evaluation of the portrayal of cloning in the media. Cloning technology has not only made a huge impact in the scientific community, but it has also affected the general community. Cloning is often misrepresented in literature and films, however, giving the public only a partial picture of the different aspects of cloning, such as religion, ethics, and government. Informing them of the facts of cloning technology will form better educated opinions. 2 Section 1 - Introduction In 1997, Dolly was created and cloning became a household word. Before this event, cloning was just a possibility, a fictional thread to weave together tales of science fiction. Once Dolly was born, however, there could be serious talk of cloning and these stories that once seemed like fiction could be considered reality. However, can stories like this really be considered reality? In most fictional books and movies, cloning starts as an amazing scientific breakthrough. However, one or more characters take advantage of the technology and attempt to create, for instance, the perfect army, or a Hitler for the current generation. It could even be as innocent as creating clones to help do a particular tedious task, but even then they get out of hand. -
Cloning of Gene Coding Glyceraldehyde-3-Phosphate Dehydrogenase Using Puc18 Vector
Available online a t www.pelagiaresearchlibrary.com Pelagia Research Library European Journal of Experimental Biology, 2015, 5(3):52-57 ISSN: 2248 –9215 CODEN (USA): EJEBAU Cloning of gene coding glyceraldehyde-3-phosphate dehydrogenase using puc18 vector Manoj Kumar Dooda, Akhilesh Kushwaha *, Aquib Hasan and Manish Kushwaha Institute of Transgene Life Sciences, Lucknow (U.P), India _____________________________________________________________________________________________ ABSTRACT The term recombinant DNA technology, DNA cloning, molecular cloning, or gene cloning all refers to the same process. Gene cloning is a set of experimental methods in molecular biology and useful in many areas of research and for biomedical applications. It is the production of exact copies (clones) of a particular gene or DNA sequence using genetic engineering techniques. cDNA is synthesized by using template RNA isolated from blood sample (human). GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) is one of the most commonly used housekeeping genes used in comparisons of gene expression data. Amplify the gene (GAPDH) using primer forward and reverse with the sequence of 5’-TGATGACATCAAGAAGGTGGTGAA-3’ and 5’-TCCTTGGAGGCCATGTGGGCCAT- 3’.pUC18 high copy cloning vector for replication in E. coli, suitable for “blue-white screening” technique and cleaved with the help of SmaI restriction enzyme. Modern cloning vectors include selectable markers (most frequently antibiotic resistant marker) that allow only cells in which the vector but necessarily the insert has been transfected to grow. Additionally the cloning vectors may contain color selection markers which provide blue/white screening (i.e. alpha complementation) on X- Gal and IPTG containing medium. Keywords: RNA isolation; TRIzol method; Gene cloning; Blue/white screening; Agarose gel electrophoresis. -
Human Cloning and the Constitution
Valparaiso University Law Review Volume 32 Number 2 Spring 1998 pp.469-542 Spring 1998 Human Cloning and the Constitution Clarke D. Forsythe Follow this and additional works at: https://scholar.valpo.edu/vulr Part of the Law Commons Recommended Citation Clarke D. Forsythe, Human Cloning and the Constitution, 32 Val. U. L. Rev. 469 (1998). Available at: https://scholar.valpo.edu/vulr/vol32/iss2/6 This Symposium is brought to you for free and open access by the Valparaiso University Law School at ValpoScholar. It has been accepted for inclusion in Valparaiso University Law Review by an authorized administrator of ValpoScholar. For more information, please contact a ValpoScholar staff member at [email protected]. Forsythe: Human Cloning and the Constitution HUMAN CLONING AND THE CONSTITUTION CLARKE D. FoRSYTHE' The thing that has saved man from his limited visions in the past has been the difficulty of devising suitable means for reaching them.' I. INTRODUCTION The recent publicity given to the cloning of a sheep in Scotland and the January 1998 declaration by a Chicago physicist, Richard Seed, that he will attempt to clone a human being in the next few years have sparked public anxiety about real or imagined dangers of human cloning and spurred efforts in Congress and many states to prohibit human cloning. The scientific and public interest is not new; this is at least the third episode of intense publicity given to cloning over the past thirty years.2 This time, however, the technology apparently exists to make human cloning a reality in the near future. -
HUMAN CLONING Papers from a Church Consultation
HUMAN CLONING Papers From a Church Consultation Evangelical Lutheran Church in America October 13-15, 2000 Chicago, Illinois Roger A. Willer, editor HUMAN CLONING Papers From a Church Consultation Roger A. Willer, editor Copyright © 2001 Evangelical Lutheran Church in America. Produced by the Department for Studies of the Division for Church in Society, 8765 W. Higgins Rd., Chicago, Illinois, 60631-4190. Permission is granted to reproduce this document as needed provided each copy carries the copyright notice printed above. Scripture quotations from the New Standard Revised Version of the Bible are copyright © 1989 by the Division of Christian Education of the National Council of Churches of Christ in the United States of America and are used by permission. Cover image © copyright 1999 PhotoDisc, Inc. The figure found on page nine is adapted from the National Institute of Health, Stem Cells: A Primer at <www.nih.gov/ news/stemcell/primer.htm>. ISBN 6-0001-3165-8. Distributed on behalf of the Division for Church in Society of the Evangelical Lutheran Church in America by Augsburg Fortress, Publishers. Augsburg Fortress order code 69-1550. This publication may be found online in its entirety as a downloadable PDF (portable document file) at <www.elca.org/ dcs/humancloning.html>. Printed on recycled paper with soy-based inks. Contents Contributors 3 Preface 4 Introduction 5 Section One, The Science and the Public Debate Kevin Fitzgerald Cloning: Can it be Good for Us? 8 Margaret R. McLean Table Talk and Public Policy Formation in the Clone Age 14 Richard Perry Broadening the Churchs Conversation 23 Section Two, Theological Resources Philip Hefner Cloning: The Destiny and Dangers of Being Human 27 Richard C. -
PER, Vol. XV, No.3, Summer 2002
........................................................................ Professional Ethics Report Publication of the American Association for the Advancement of Science Scientific Freedom, Responsibility & Law Program in collaboration with Committee on Scientific Freedom & Responsibility Professional Society Ethics Group VOLUME XV NUMBER 3 SUMMER 2002 Intellectual Property and Traditional Ecological nity. It does not belong to any single individual within the commu- Knowledge: Institutionally Globalized Biopiracy? nity, and is transmitted through specific cultural and traditional information exchange mechanisms. Traditional knowledge is often By Stephen A. Hansen maintained and transmitted orally through elders or specialists (breeders, healers, etc.), and often to only a select few people within Stephen A. Hansen, M.A. is a Senior Program Associate with a community. the Science and Human Rights Program in the Directorate for Science and Policy Programs of the AAAS, and is the Project The knowledge of and uses of specific plants for medicinal pur- Director for the Traditional Ecological Knowledge Project. He poses is an important component of traditional knowledge systems. developed the Traditional Ecological Knowledge Prior Art Once, this knowledge was a major source of materials and informa- Database (T.E.K.*P.A.D.), an index and search engine of tion for the development of pharmaceuticals. But in the 20th century, existing Internet-based, public domain documentation concern- new sources for antibiotics derived from soil cultures and