CURRICULAM VITAE Dr. Pratima Chaudhuri NAME

Professor, Amity Institute of PRESENT POSITION AND Biotechnology, Amity AFFILIATION University, NOIDA, UP, INDIA Amity Institute of Biotechnology, Sector-125, NOIDA, UP- CONTACT INFORMATION 121313, INDIA, Email:pratimachaudhuri@ya hoo.com Tel: +91-9873272506

• Recombinant production and characterization of therapeutically important proteins INTERESTS • Equilibrium, Kinetics and Thermodynamics of protein

folding reactions Specialization in Chemistry, Chemical Biology, and Structural Biology • Effect of nanoparticles in protein conformational changes and enhancement of recombinant protein preparation in vivo and in vitro

EDUCATIONAL QUALIFICATIONS: Name of College / University Degree Year

University of Calcutta, India Ph.D., Chemistry 1995, University of Calcutta, India M.Sc., Inorganic 1988, Chemistry

University of Calcutta, India B.Sc., Chemistry 1984, (Honors)

Title of Ph.D. thesis: “Thermodynamic and kinetic studies on the formation of some ternary complexes of Co(II) and Ni(II)”

EXPERIENCE IN RESEARCH (in chronological order) Designation Type of post Name of the Institute Year (From – To) held (teaching/ research) Ph.D Scholar Research Department of Chemistry, 1989-1995 University of Calcutta, CURRICULAM VITAE Postdoctoral Research Chiba Institute of Technology, 1997-1998 Research Chiba, Japan Fellow JSPS Funded

Postdoctoral Research Yale University, USA 1999-2001 Research NIH funded Fellow Postdoctoral Research University of Utah, Salt lake city, 2001-2002 Research NIH funded USA Fellow and Adjunct Faculty Postdoctoral Research IIT Delhi 2003-2004 Research DBT funded Fellow Professor Teaching and Amity University, Noida Campus 2004- Present Research

Teaching : In UG, PG and Ph.D Courses 1. Chemical Biology 2. Animal Biotechnology 3. Ph.D (Research techniques) 4. Chemistry (Coordinator)

Awarded: Two

1. Name: Mrs Charu Thypliyal (November,2015) Title of thesis: structure function relationship and conformational properties of recombinant proteins: dihydrofolate reductase

2. Name: Ms Naira Rashid (December,2018) Title of thesis :“Chemical and Molecular Chaperone Assisted Protein Folding of Recombinant DHFR”

Ongoing: Five [Three (Full Time) and Two (Part Time)] Ph.D. student supervision 1. Name: Ms Twinkle (Part time Student from IARI, New Delhi) (Batch 2016-2020) Title of thesis : phylogenetic and taxonomic analysis of leaf eating semi-loopers belonging to subfamily plusiinae (lepidoptera: noctuidae)

2. Name: Ms Ritu Verma (Full Time) (Batch 2018-2021) Title of thesis : “Nanoparticle induced functional and conformational correlation of recombinant Dihydrofolate reductase protein”

3. Name : Mrs Preeti Gupta (Full time) (Batch 2018-2021) Title of thesis : UNDERSTANDING THE ROLE OF NANO PARTICLES IN THE FOLDING, STABILITY AND AGGREGATION PROPERTIES OF DIHYDROFOLATE REDUCTASE (DHFR) PROTEIN

CURRICULAM VITAE

4. Name: Vaishali Achariya (Full Time) (Batch 2019-2022) Title of thesis : STUDYING UNFOLDING OF ZEBRAFISH DIHYDROFOLATE REDUCTASE PROTEIN BY VARIOUS DENATURANTS AND EVALUATION OF THE ROLE OF DENATURED STATE IN IT’S REFOLDING YIELD

5. Name: Mrs Shikha Chaudhary (Part time student from AIIMS, New Delhi) (Batch2020-2024) joined on July,2020

1. DBT Funded research project: “Nanoparticle mediated initiatives for enhancement of recombinant DHFR protein-e-coli and cytosol: A biotechnology approach” sanctioned by DBT of RS. 42,89,800. (2017 –2020) ; Project No. BT/PR21923/NNT/28/1225/2017 for 3 years (Role: PI of this project), Ongoing

RESEARCH 2. CSIR funded research Project: Title: “Developing assisted folding processes in PROJECTS recombinant proteins for production enhancement”. Sanctioned on 1st April. 2011 for 3 Completed: (2) years (2011- 2014) Budged : ~ 18.23 Lacs No. 37(1461)/11/EMR-11 ; (Role: PI of this Ongoing: (1) project ) Completed

3. DST funded research project in the Womens Scientist Scheme. Title: “Solving the issue of degradation of purified urokinase from human kidney cell line and enhancing the life time of fully active preparation “Santioned on November 2004-2007; Budget: 15 lacs; No. SR/WOS-A/LS-114/2004 ( Role: PI of this project) Completed

1. “Stability and refolding of Dihydrofolate reductase enhanceswith nano-conjugation”, Ritu Verma, Neetu Singh, Pratima Chaudhuri ("Chattopadhyay"))*, International Journal of Biological Macromolecules (2020), doi: 10.1016/j.ijbiomac.2020.11.053. 5.162

2. “A versatile tool in controlling aggregation and Ag nanoparticles assisted in vitro folding of thermally denatured zDHFR”, Preeti Gupta, Ritu Verma, Anita Verma, and Pratima Chaudhuri (Chattopadhyay)*, Biochemistry and Biophysics Reports, Volume 24, December; 2020, 100856; Impact factor 1.65 Scopus

3. DNA barcoding and Taxonomic account on some selected species of subfamily Plusiinae Publications (Lepidoptera: Noctuidae) from India.” Twinkle, PR Shashank, PC Chattopadhyay; Zootaxa 4845 (4): 451-486, 2020 Impact factor 1.030 Scopus

4. Genetic analysis of green garden looper, Chrysodeixis eriosoma (Lepidoptera: Noctuidae: Plusiinae) using mitochondrial COI gene sequences from India; Twinkle, PR Shashank, PC Chattopadhyay; - Journal of Experimental Biology and Agricultural Sciences 2020 Vol.8 No.3 pp.269-275, 2020; DOI : 10.18006/2020.8(3).269.275 Scopus

5. "Evolutionarily related dihydrofolate reductases perform coequal functions yet show divergence in their trajectories" N Rashid, P Chaudhuri (Chattopadhyay)*, The Protein Journal: August, 37(4) 301-310, 2018. Impact Factor 2.499 ; ISSN 1572-3887; Scopus

CURRICULAM VITAE 6. “Quantification of differential efficacy of chemical chaperones in ameliorating solubilization and folding of zebrafish dihydrofolate reductase” N Rashid, C Thapliyal, P Chaudhuri (Chattopadhyay)*, International journal of biological macromolecules: May; 111:186- 192, 2018; citation 1 ; ISSN 1879-0003; Impact factor 5.162; Scopus

7. “Kinetics and thermodynamics of the thermal inactivation and chaperone assisted folding of zebrafish dihydrofolate reductase” C Thapliyal, N Jain, N Rashid, P Chaudhuri (Chattopadhyay)*, Archives of biochemistry and biophysics 637, 21-30, 2018 ; citation 3 ; ISSN 0003-9861; Impact factor 3.017 Scopus

8. DNA barcoding and morphological characterization of moth Antoculeora ornatissima (Walker, 1858)(Lepidoptera: Noctuidae), a new range record from western Himalayan region of India; T Sinha, PR Shashank, PC Chattopadhyay; Journal of Threatened Taxa 10 (13), 12817-12820; 2018 Citation 1 Impact factor 0.440 Scopous

9. “Osmolyte induced enhancement of expression and solubility of human dihydrofolate reductase: An in vivo study” Naira Rashid, Charu Thapliyal, Pratima Chaudhuri (Chattopadhyay),* International Journal of Biological Macromolecules, 103, 1044- 1053, 2017; Citation 4; ISSN 1879-0003; Impact factor 5.162 Scopus

10. “Dihydropholate Reductas As A Versatile drug target in health care” Naira Rashid, Charu Thapliyal and Pratima Chaudhuri (Chattopadhyay)*; Journal of proteins and Proteomics; 7( 4), 2016, pp. 247-257 Citation 10 ; ISSN 2524-4663 Impact factor 2.539 Scopus

11. “Investigation of folding unfolding process of a new variant of Dihydrofolate reductase protein from Zebrafish” Charu Thapliyal, Neha Jain, Pratima Chaudhuri (Chattopadhyay)*, International Journal of Biological Macromolecules, (91) 736-743, 2016 citation 5; ISSN 1879-0003; Impact factor 5.162; Scopus

12. “Comparison of physico-chemical aspects between E.coli and Human DihydrofolatReductase:An equilibrium unfolding study” Charu Thapliyal, Neha Jain, and Pratima Chaudhuri (Chattopadhyay)* Biophysics, (No 3, Vol 60 ), 2015 citation 3 ; ISSN 0006-3029; Impact factor 3.854 Scopus

13. “Effect of various osmolytes on expression and functionality of Zebrafish Dihydrofolate reductase: an in vivo study” Charu Thapliyal and Pratima Chaudhuri (Chattopadhyay)* JOURNAL OF PROTEINS AND PROTEOMICS ; (No 2, Vol 16) pp. 211-218; 2015 citation 12; ISSN 2524-4663 Impact factor 2.539 Scopus

14. “Structural Characterization of the Mechanosensitive Channel Candidate MCA2 from Arabidopsis thaliana” Hideki Shigematsu, Kazuko Iida, Masataka Nakano, Pratima Chaudhuri, Hidetoshi Iida, Kuniaki Nagayama; PLOS ONE ; Volume 9, e87724; Issue 1, January 2014 ; Impact 3.77 citation 21; ISSN 1932-6203; Scopus

15. “Structure-activity correlationship and folding of recombinant Escherichia coli dihydro folate reductase (DHFR) enzyme through biochemical and biophysical approaches. Jai Mittal, Gayathri Ravitchandirane, Pratima Chaudhuri (Chattopadhyay) Journal of CURRICULAM VITAE Biophysical chemistry, Vol.1, No.2, 694-706 (2010). Citation 1; ISSN 2153-0378; Impact Factor 0.58;

16. “A global survey of haplotype frequencies and linkage disequilibrium at the RET locus”, Pratima Chattopadhyay, Andy J. Pakstis, Namita Mukherjee, Judith R. Kidd, Andy Odunsi, Fred Okonofua and Kenneth K. Kidd. European Journal of Human Genetics, 11, pp 760-769, 2003 ; citation 20, ISSN 1018-4813 Impact factor 4.349 Scopus

17. “Structural requirement for the Two-Step Dimerization of Human Immuno Deficiency Virus Type-1 Genome”, Ken-ichi Takahashi, Seiki Baba, Pratima Chattopadhyay, Yoshio Koyanagi, Naoki Yamamoto, Hiroshi Takaku, and Gota Kawai. RNA, vol.6, No. 1, pp 96- 102, 2000. Citation 127; ISSN 1355-8382; Impact factor 4.94 Scopus

18. “An NMR and mutational analysis of an RNA pseudoknot of E.coli tmRNA involved in trans-translation” Nobukazu Nameki, Pratima chattopadhyay, Hyouta Himeno, Akira Muto, and Gota Kawai. ; Nucleic Acids Res. Vol. 27, No. 18, pp 1-9, (1999) ; Citation 52; ISSN 2311-3278; Impact factor 11.14 Scopus

19. “Dynamics of complex formation in M(II(-Nitrilo-Triacetate-L systems (M- Cobalt(II),Nickel(II); L-SCN,Bipy,Phen”,.Pratima Chattopadhyay, and D. Banerjea, Polyhedron, Vol 13, Page 1981-1988, (1994) Citation 8 ; Impact factor 2.067; ISSN 0277-5387 Scopus

Google scholar: https://scholar.google.com/citations?hl=en&user=aHvCvdsAAAAJ&view_op=list_works &sortby=pubdate

• Sir Rash Behari Ghose Research Award for the Doctoral Research, University of Calcutta, India, 1990-1994

• Japanese Society for the Promotion of Science Awards (JSPS) sponsored Postdoctoral Fellowship, (Chiba Institute of Technology, JAPAN) 1997-1998.

• NIH funded postdoctoral fellowship (Yale University and Salt Lake City, USA), 1999-2002

1. “Osmolyte System and Its Biological Significance” P Chaudhuri (Chattopadhyay), N Rashid, C Book Chapters Thapliyal; Cellular Osmolytes, 1-34, 2017 Springer Nature Publication; 978-981-10-3707-8

2. “HSP 60 as modulators of Apoptosis” P Chaudhuri (Chattopadhyay), N Rashid, “Heat Shock Protein 60 in Human Diseases and Disorders” ; 2019 Springer Nature Publication ; 978-3-030- 23154-5

MEMBERSHIP 1.Association of Microbiologists of India (AMI) (Life Member) with Professional/ (From 2005) Academic bodies 2. International Membership: Athens Institute of education and CURRICULAM VITAE Research, Antiner, Greece (Member for Chemistry and Biology) (From 2016) 3. Indian Society of Translational Research, India ( Life Member) (From 2017)

1. Title: Process for the synthesis of AA-dUTP-Cy3/Cy5- a novel fluorescent labeling agent Name of the inventor: Pratima Chaudhuri. Ref: 1529/DEL/2009; National Status: Granted Indian patent on 30/12/2019 PATENTS 2. Title: “Efficient protein folding process to improve the functional yield of recombinant zDHFR protein” Name of the inventor: Charu Thapliyal, Pratima Chaudhuri, Ref: 2170/DEL/2015; (National); Status: Filed RFE on 17/07/2019

1. 4Th Annual International Conference ; NANOBIOTECK – 2018 , 21st – 23rd November, 2019, at Aerocity, New Delhi

2. 3rd Annual International Conference, NANOBIOTECK – 2018 , 24th - 27th October 2018, at AIIMS, New Delhi

3. Protein Folding Dynamics Conference organised by Gordon Research Conferences at Texas, USA (January, 2018).

4. 3rd International Conference on Translational Research, Amity University, Kolkata September, 2017).

Important 5. “NMR analysis of the interaction between SL3.2 in SL5B coding region and the single stranded region located 200 nt upstream in the HCV genome RNA” Yoki Otomo, Pratima International Chaudhuri, Gota Kawai; The 53rd Annual Meeting of the NMR Society of Japan, Nov, 4-6th, Conferences 2014, Osaka University, Japan.

6. “NMR study on the conformational conversion of the dimerization initiation site of the HIV- 1 genomic RNA”.Misaki Imai, Pratima Chaudhuri, Amiu Shino and Gota Kawai*; 2010; Proceedings of ISNAC, Tokyo, Japan

7. International Interdisciplinary Science Conference (IISC-2012) on “protein folding and Diseases” at Jamia millia Islamia, New Delhi, during 8-10 December.2012 8. World Congress of Biotechnology” at Leonia International Convention Centre, Hyderabad, India during 4-6 may, 2012. 9. “2nd International Conference on Perspectives of Cell signaling and Molecular Medicine, January 8-11, 2012, Bose Institute, Kolkata.

CURRICULAM VITAE • “Mini-GroEL assist in Vivo and in Vitro folding of large natural substrate proteins” Date and Venue: Chiba Institute of Technology, Chiba, Japan, 17th February, 2015

• “Biophysical characterisation of Recombinant Zebra Fish DHFR by unfolding and refolding studies”. Date and Venue: Chiba Institute of Technology, Chiba, Japan, 26th March, 2014

Invited Lecture • A large scale membrane protein(MCA2) production based on an insect cell culture” Date ( International) and Venue: National Institute for Physiological Sciences (NIPS), Okazaki, Japan, June, 2008

• “Production and characterization of Tissue plasminogen activator from human Kidney cell line” Date andVenue : Tsukuba University, Ibaraki, Japan, 15th June,2007.

• “Study on the conformational change of the dimerization initiation site from HIV-1 genomic RNA”. Date and Venue: Chiba Institute of Technology, Chiba, Japan, 25th June, 2007