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ACTL6A Antibody (N-Term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap21525a

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ACTL6A Antibody (N-Term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap21525a 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 ACTL6A Antibody (N-term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP21525a Specification ACTL6A Antibody (N-term) - Product Information Application WB,E Primary Accession O96019 Reactivity Human, Mouse Host Rabbit Clonality polyclonal Isotype Rabbit Ig Calculated MW 47461 ACTL6A Antibody (N-term) - Additional Information Gene ID 86 Other Names Actin-like protein 6A, 53 kDa BRG1-associated factor A, Actin-related All lanes : Anti-ACTL6A Antibody (N-term) at protein Baf53a, ArpNbeta, BRG1-associated 1:2000 dilution Lane 1: mouse skeletal factor 53A, BAF53A, INO80 complex subunit muscle lysates Lane 2: K562 whole cell K, ACTL6A, BAF53, BAF53A, INO80K lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Target/Specificity Peroxidase conjugated at 1/10000 dilution This ACTL6A antibody is generated from a rabbit immunized with a KLH conjugated Predicted band size : 47 kDa synthetic peptide between 55-89 amino Blocking/Dilution buffer: 5% NFDM/TBST. acids from the N-terminal region of human ACTL6A. ACTL6A Antibody (N-term) - Background Dilution WB~~1:2000 Involved in transcriptional activation and repression of select genes by chromatin Format remodeling (alteration of DNA-nucleosome Purified polyclonal antibody supplied in PBS topology). Required for maximal ATPase with 0.09% (W/V) sodium azide. This activity of SMARCA4/BRG1/BAF190A and for antibody is purified through a protein A association of the SMARCA4/BRG1/BAF190A column, followed by peptide affinity containing remodeling complex BAF with purification. chromatin/nuclear matrix. Belongs to the neural progenitors- specific chromatin Storage remodeling complex (npBAF complex) and is Maintain refrigerated at 2-8°C for up to 2 required for the proliferation of neural weeks. For long term storage store at -20°C progenitors. During neural development a in small aliquots to prevent freeze-thaw switch from a stem/progenitor to a post- cycles. mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and Precautions become committed to their adult state. The ACTL6A Antibody (N-term) is for research transition from proliferating neural Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 use only and not for use in diagnostic or stem/progenitor cells to post-mitotic neurons therapeutic procedures. requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into ACTL6A Antibody (N-term) - Protein Information neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative Name ACTL6A ACTL6B/BAF53B and DPF1/BAF45B or Synonyms BAF53, BAF53A, INO80K DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is Function essential for the self-renewal/proliferative Involved in transcriptional activation and capacity of the multipotent neural stem cells. repression of select genes by chromatin The nBAF complex along with CREST plays a remodeling (alteration of DNA-nucleosome role regulating the activity of genes essential topology). Component of SWI/SNF for dendrite growth (By similarity). Component chromatin remodeling complexes that carry of the NuA4 histone acetyltransferase (HAT) out key enzymatic activities, changing complex which is involved in transcriptional chromatin structure by altering DNA-histone activation of select genes principally by contacts within a nucleosome in an acetylation of nucleosomal histones H4 and ATP-dependent manner. Required for H2A. This modification may both alter maximal ATPase activity of nucleosome - DNA interactions and promote SMARCA4/BRG1/BAF190A and for interaction of the modified histones with other association of the SMARCA4/BRG1/BAF190A proteins which positively regulate containing remodeling complex BAF with transcription. This complex may be required chromatin/nuclear matrix. Belongs to the for the activation of transcriptional programs neural progenitors-specific chromatin associated with oncogene and proto-oncogene remodeling complex (npBAF complex) and mediated growth induction, tumor suppressor is required for the proliferation of neural mediated growth arrest and replicative progenitors. During neural development a senescence, apoptosis, and DNA repair. NuA4 switch from a stem/progenitor to a may also play a direct role in DNA repair when postmitotic chromatin remodeling recruited to sites of DNA damage. Also mechanism occurs as neurons exit the cell involved in vitamin D-coupled transcription cycle and become committed to their adult state. The transition from proliferating regulation via its association with the WINAC neural stem/progenitor cells to postmitotic complex, a chromatin-remodeling complex neurons requires a switch in subunit recruited by vitamin D receptor (VDR), which is composition of the npBAF and nBAF required for the ligand- bound VDR-mediated complexes. As neural progenitors exit transrepression of the CYP27B1 gene. Putative mitosis and differentiate into neurons, core component of the chromatin remodeling npBAF complexes which contain INO80 complex which is involved in ACTL6A/BAF53A and PHF10/BAF45A, are transcriptional regulation, DNA replication and exchanged for homologous alternative probably DNA repair. ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific ACTL6A Antibody (N-term) - References complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative Zhao K.,et al.Cell 95:625-636(1998). capacity of the multipotent neural stem Harata M.,et al.Biosci. Biotechnol. Biochem. cells. The nBAF complex along with CREST 63:917-923(1999). plays a role regulating the activity of genes Harata M.,et al.Submitted (APR-2001) to the essential for dendrite growth (By similarity). EMBL/GenBank/DDBJ databases. Component of the NuA4 histone Wiemann S.,et al.Genome Res. acetyltransferase (HAT) complex which is 11:422-435(2001). involved in transcriptional activation of Ota T.,et al.Nat. Genet. 36:40-45(2004). select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of Page 2/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Cellular Location Nucleus. ACTL6A Antibody (N-term) - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture Page 3/3 Powered by TCPDF (www.tcpdf.org).
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