DOCSLIB.ORG

Effects of Intranasally Administered Dnsp-11 on the Central Dopamine System of Normal and Parkinsonian Fischer 344 Rats

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Effects of Intranasally Administered Dnsp-11 on the Central Dopamine System of Normal and Parkinsonian Fischer 344 Rats University of Kentucky UKnowledge Theses and Dissertations--Neuroscience Neuroscience 2013 EFFECTS OF INTRANASALLY ADMINISTERED DNSP-11 ON THE CENTRAL DOPAMINE SYSTEM OF NORMAL AND PARKINSONIAN FISCHER 344 RATS James H. Sonne University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Sonne, James H., "EFFECTS OF INTRANASALLY ADMINISTERED DNSP-11 ON THE CENTRAL DOPAMINE SYSTEM OF NORMAL AND PARKINSONIAN FISCHER 344 RATS" (2013). Theses and Dissertations-- Neuroscience. 5. https://uknowledge.uky.edu/neurobio_etds/5 This Doctoral Dissertation is brought to you for free and open access by the Neuroscience at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Neuroscience by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained and attached hereto needed written permission statements(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine). I hereby grant to The University of Kentucky and its agents the non-exclusive license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known. I agree that the document mentioned above may be made available immediately for worldwide access unless a preapproved embargo applies. I retain all other ownership rights to the copyright of my work. I also retain the right to use in future works (such as articles or books) all or part of my work. I understand that I am free to register the copyright to my work. REVIEW, APPROVAL AND ACCEPTANCE The document mentioned above has been reviewed and accepted by the student’s advisor, on behalf of the advisory committee, and by the Director of Graduate Studies (DGS), on behalf of the program; we verify that this is the final, approved version of the student’s dissertation including all changes required by the advisory committee. The undersigned agree to abide by the statements above. James H. Sonne, Student Dr. Don Marshall Gash, Major Professor Dr. Wayne A. Cass, Director of Graduate Studies EFFECTS OF INTRANASALLY ADMINISTERED DNSP-11 ON THE CENTRAL DOPAMINE SYSTEM OF NORMAL AND PARKINSONIAN FISCHER 344 RATS _______________________________________ DISSERTATION _______________________________________ A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Medicine at the University of Kentucky By James William Hendry Sonne Lexington, Kentucky Director: Don Marshall Gash, Ph.D., Professor of Anatomy and Neurobiology Lexington, Kentucky 2013 Copyright © James William Hendry Sonne ABSTRACT OF DISSERTATION EFFECTS OF INTRANASALLY ADMINISTERED DNSP-11 ON THE CENTRAL DOPAMINE SYSTEM OF NORMAL AND PARKINSONIAN FISCHER 344 RATS Due to the blood-brain barrier, delivery of many drugs to the brain has required intracranial surgery which is prone to complication. Here we show that Dopamine Neuron Stimulating Peptide 11 (DNSP-11), following non-invasive intranasal administration, protects dopaminergic neurons from a lesion model of Parkinson’s disease in the rat. A significant and dose-dependent increase in an index of dopamine turnover (the ratio of DOPAC to dopamine) was observed in the striatum of normal young adult Fischer 344 rats by whole-tissue neurochemistry compared to vehicle administered controls. Among animals challenged with a moderate, unilateral 6-hydroxy- dopamine (6-OHDA) lesion of the substantia nigra, those treated repeatedly with intranasally administered DNSP-11 exhibited greater numbers of tyrosine hydroxylase (TH) positive dopaminergic neuronal cell bodies in the substantia nigra and greater TH+ fiber density in the striatum when compared to animals treated intranasally with vehicle only or a scrambled version of the DNSP-11 sequence. Lesioned animals that received intranasal DNSP-11 treatment did not exhibit abnormal, apomorphine-induced rotation behavior, contrasted with animals that received only vehicle or scrambled peptide that did exhibit significantly greater rotation behavior. In addition, the endogenous expression of DNSP-11 from the pro-region of GDNF was investigated by immunohistochemistry with a custom, polyclonal antibody. Signal from the DNSP-11 antibody was found to be differentially localized from the mature GDNF protein both spatially and temporally. While DNSP-11-like immunoreactivity extensively colocalizes with GDNF immunoreactivity at post-natal day 10, the day of maximal GDNF expression, DNSP-11-like signal was found to be present in the 3 month old rat brain with signal in the substantia nigra, ventral thalamic nucleus, dentate gyrus of the hippocampus, with the strongest signal observed in the locus ceruleus where GDNF is not expressed. Results from immunoprecipitation of brain homogenate were not consistent with the synthetic, amidated 11 amino-acid rat DNSP-11 sequence. However, binding patterns in the literature of NPY, the only homologous sequence present in the CNS, do not recapitulate the immunoreactive patterns observed for the DNSP-11 signal. This study provides evidence for a potential easy-to-administer intranasal therapeutic using the DNSP-11 peptide for protection from a 6-OHDA lesion rat model of Parkinson’s disease. KEYWORDS: Parkinson’s disease, GDNF, 6-OHDA, aging, non-invasive James William Hendry Sonne May 3rd, 2013 EFFECTS OF INTRANASALLY ADMINISTERED DNSP-11 ON THE CENTRAL DOPAMINE SYSTEM OF NORMAL AND PARKINSONIAN FISCHER 344 RATS By James William Hendry Sonne Don Marshall Gash, Ph.D. Director of Dissertation Wayne A. Cass, Ph.D. Director of Graduate Studies May 3rd, 2013 For my grandparents, Jody Tharp and Lloyd Gould Hendry ACKNOWLEDGEMENTS I am truly grateful for the help and invaluable assistance I have received, without which this work would not have been feasible. My success is truly not my own. First and foremost, I am indebted to my mentor, Don Marshall Gash, Ph.D., for his support, faith and confidence in me, as well as his friendship. Before beginning my endeavors, when I envisioned a translational research laboratory, it was the goals and motivations that he shares which were the core of my vision. It is difficult to imagine how I can take a step upward from his laboratory. My committee members have provided excellent guidance, and I appreciate them providing me with resources and taking the time out of their productive schedules to support my progress. They are Michael Bardo, Ph.D., Luke H. Bradley, Ph.D., Wayne A. Cass, Ph.D., James Geddes, Ph.D., Greg A. Gerhardt, Ph.D., Zhiming Zhang, M.D., my outside examiner John T. Slevin, M.D., and Susan Barron, Ph.D. for acting as a substitute committee member for my defense. I would like to especially thank Yi Ai, M.D., Wayne Cass, Ph.D., Richard Grondin, Ph.D., and Zhiming Zhang, M.D. They are fantastic investigators and sincere and caring people who have provided technical assistance and a deep interest in my development as a scientist. I would also like to thank the teaching faculty, especially April D. Richardson-Hatcher, Ph.D. and Bruce E. Maley, Ph.D., for helping me to become a better instructor and exposing me to the joy of helping students succeed. The members of Dr. Gash’s laboratory and his collaborating laboratories have been more than simply invaluable resources, but true friends that I know I can trust. My fellow denizens of the laboratory have been tremendous in their technical assistance and comic relief, especially Ramsey Edwards, M.B.A., Eric Forman, Hamed Haghnazar, M.P.H., April Evans, Ryan Weeks, Daisy Ramos, Ofelia Meagan Littrell, Ph.D. and Jennifer Moorehead. Most of all, Mallory J. Stenslik, M.S. has provided countless hours of laughs and teamwork. These people have kept me going during tough times. iii I am indebted to my grandparents, Jody T. and Lloyd G. Hendry, J.D., for teaching me so much about life. They instilled in me the meaning of hard work, devotion, and the importance of taking joy in a job well done, and taught me that without these things success is not possible. I believe that every act they performed was directed at making life better for their grandchildren. Their way of looking forward is what made them successful, and it has influenced my life beyond measure. They are my role models in every aspect of life, while my parents, Mary C. H. Sonne, J.D., and Kenneth G. Sonne, Jr., have provided the support to allow me to pursue my dreams wherever they lead. Above all of these, I owe my wife, Stephanie Lynn Sonne, C.P.A., everything. During my long hours and tribulations, she has stood by me, supporting me when I needed it the most. Without her love, I would be nothing. These people have been my fuel, the oil in my engine, and the tread on my tires. My successes and accomplishments are because of them, while my missteps are purely mine own. iv TABLE OF CONTENTS ACKNOWLEDGEMENTS ..................................................................................... iii LIST OF TABLES .................................................................................................ix LIST OF FIGURES ..............................................................................................
Load more

Recommended publications
  • Fall 2010 U.S
    178451_Cover_B.qxd:178451_Cover_B 12/6/10 10:04 PM Page 1 Nonprofit Org. FALL 2010 U.S. Postage IN THIS ISSUE FALL 2010 FALL 421 Mondale Hall PAID New Environmental Courses • Q&A: Anderson & Rosenbaum • Super CLE Week • Don Marshall Tribute 229 19th Avenue South Minneapolis, MN Minneapolis, MN 55455 Permit No. 155 Perspectives E NVIRONMENTAL C APRIL 15—16, 2011 OURSES • Q&A: A PLEASE JOIN US AS WE CELEBRATE THE LAW SCHOOL AND ITS ALUMNI IN A WEEKEND OF ACTIVITIES FOR THE ENTIRE LAW SCHOOL COMMUNITY. NDERSON Friday, April 15: All-Alumni Cocktail Reception Saturday, April 16: Alumni Breakfast & CLE & R OSENBAUM SPECIAL REUNION EVENTS WILL BE HELD FOR THE CLASSES OF: 1961, 1971, 1976, 1981, 1986, 1991, 1996, 2001, and 2006 • CLE • D FOR ADDITIONAL INFORMATION, OR IF YOU ARE INTERESTED IN PARTICIPATING IN ON M THE PLANNING OF YOUR CLASS REUNION, PLEASE CONTACT EVAN P. JOHNSON, ARSHALL Alumni Relations & Annual Giving Program Manager T 612.625.6584 or [email protected] RIBUTE Spring Alumni Weekend is about returning to remember your years at the Law School and the friendships you built here. We encourage those of you with class reunions in 2011 to “participate in something great” by making an increased gift or pledge to the Law School this year. Where the Trials Are www.law.umn.edu WWW.COMMUNITY.LAW.UMN.EDU/SAW Criminal law is challenging but satisfying, say alumni from all sides of the courtroom. 178451_Cover_B.qxd:178451_Cover_B178451_Cover_B.qxd:178451_Cover_B 12/6/10 12/6/10 10:04 10:04 PM PagePM Page2 2 178451_Section A FrMatter.qxd:178451_Section A FrMatter 12/3/10 11:56 AM Page 1 Securing Our Future his fall we welcomed 260 first-year students, along with 36 LL.M.
    [Show full text]
  • Interview with Paul Findley # IS-A-L-2013-002 Interview # 1: January 15, 2013 Interviewer: Mark Depue
    Interview with Paul Findley # IS-A-L-2013-002 Interview # 1: January 15, 2013 Interviewer: Mark DePue The following material can be used for educational and other non-commercial purposes without the written permission of the Abraham Lincoln Presidential Library. “Fair use” criteria of Section 107 of the Copyright Act of 1976 must be followed. These materials are not to be deposited in other repositories, nor used for resale or commercial purposes without the authorization from the Audio-Visual Curator at the Abraham Lincoln Presidential Library, 112 N. 6th Street, Springfield, Illinois 62701. Telephone (217) 785-7955 Note to the Reader: Readers of the oral history memoir should bear in mind that this is a transcript of the spoken word, and that the interviewer, interviewee and editor sought to preserve the informal, conversational style that is inherent in such historical sources. The Abraham Lincoln Presidential Library is not responsible for the factual accuracy of the memoir, nor for the views expressed therein. We leave these for the reader to judge. DePue: Today is Tuesday, January 15, 2013. My name is Mark DePue. I’m the Director of Oral History with the Abraham Lincoln Presidential Library. Today I’m in Jacksonville, Illinois, specifically, at Illinois College, Whipple Hall. I’m with Congressman Paul Findley. Good morning, sir. Findley: Good morning. DePue: I’ve been looking forward to this interview. I’ve started to read your autobiography. You’ve lived a fascinating life. Today I want to ask you quite a bit to get your story about growing up here in Jacksonville and your military experiences during World War II, and maybe a little bit beyond that, as well.
    [Show full text]
  • February Is Heart Month!
    The Official Monthly Publication of the Castle Rock Senior Activity Center February is Heart Month! We’ll help you keep your heart happy. MONDAY - FRIDAY 8:30 AM - 4:30 PM 2323 Woodlands Blvd., Castle Rock, CO 80104 Office: 303.688.9498 Fax: 303.814.1035 WHAT’S INSIDE From the Director With February being the month of love and romance, I thought PAGE I would share the staff’s favorite romance movies and quotes. Recognitions & Volunteering 3 If you haven’t watched these, you might want to check them Rockworthy 4 out. Who doesn’t love curling up on the couch and getting Road to Wellness 5 lost in a good romance movie? Ok guys, maybe not you. Fundraising Rocktivities 6 There have been some good romance comedies or sport Advertisements 7 movies with a little romance thrown in. We would love to hear Rockin’ Happenings 8 from you. Send us an email at [email protected] and let us More Rockin’ Happenings 9 know what your favorites are. Groups & Games 10 Debbi’s favorite romance movie is Dirty Dancing. Her favorite quote is “You had me at Of Interest 11 Hello” from Renee Zellweger’s character to Tom Cruise in Jerry Maguire. Calendar 12-13 Boredom Busters 14-15 Tina’s favorite romance movie is Sense and Sensibility. Her favorite quote from that is “I What’s Going on This Month 16 do not attempt to deny that I think very highly of him, that I.. greatly esteem him.. I like Advertisements 17 him” by Elinor Dashwood (Emma Thomson) when talking to Maryanne (Kate Winslet) about Edward (Hugh Grant).
    [Show full text]
  • Acupuncture and Traditional Chinese Medicine in the Treatment of Parkinson’S Disease
    Schulz Capstone Acupuncture and Traditional Chinese Medicine In the Treatment of Parkinson’s Disease By Mary M. Schulz Presented in partial fulfillment for the Doctor of Acupuncture and Oriental Medicine Degree Capstone Advisors: Eric Tamrazian, M.D., Lawrence J. Ryan, Ph.D. Yo San University April 2014 Schulz Capstone Approvals Signatures Page This Capstone Project has been reviewed and approved by: _________________________________________ __5/4/2015__________ Eric Tamrazian, M.D., Capstone Project Advisor Date _________________________________________ _5/4/2015____________ Lawrence Ryan, Ph.D., Capstone Project Advisor Date _________________________________________ __5/4/2015___________ Don Lee, L.Ac., Specialty Chair Date _________________________________________ __5/4/2015____________ Andrea Murchison, DAOM, L.Ac., Program Director Date 2 Schulz Capstone ABSTRACT Research has shown that arresting progress of disease by early intervention is paramount to preventing its progression. Recent studies reveal early signs and symptoms of Parkinson’s Disease (PD) to be anosmia (loss of smell), constipation and REM Sleep Disorder and are confirmed to develop up to ten years prior to the more well known, and classical diagnosed, motor symptoms of resting tremor, bradykinesia, and rigidity. Motor symptoms in PD appear as a result of the progressive loss of dopamine in the basal ganglia, particularly within the substantia nigra, pars compacta region. By the time PD motor symptoms develop and are diagnosed, an estimated 80% of striatal nerve terminals and 60% of dopaminergic neurons have already been lost. Modern biomedical intervention for motor symptoms primarily focuses on the use of the pharmaceutical combination of carbidopa/levodopa (L-Dopa therapy). It is well known L- Dopa therapy has a waning period after 3 to 5 years of use, with up to 50% of patients developing dyskinesias as a result of this treatment.
    [Show full text]
  • NIDA Drug Supply Program Catalog, 25Th Edition
    RESEARCH RESOURCES DRUG SUPPLY PROGRAM CATALOG 25TH EDITION MAY 2016 CHEMISTRY AND PHARMACEUTICS BRANCH DIVISION OF THERAPEUTICS AND MEDICAL CONSEQUENCES NATIONAL INSTITUTE ON DRUG ABUSE NATIONAL INSTITUTES OF HEALTH DEPARTMENT OF HEALTH AND HUMAN SERVICES 6001 EXECUTIVE BOULEVARD ROCKVILLE, MARYLAND 20852 160524 On the cover: CPK rendering of nalfurafine. TABLE OF CONTENTS A. Introduction ................................................................................................1 B. NIDA Drug Supply Program (DSP) Ordering Guidelines ..........................3 C. Drug Request Checklist .............................................................................8 D. Sample DEA Order Form 222 ....................................................................9 E. Supply & Analysis of Standard Solutions of Δ9-THC ..............................10 F. Alternate Sources for Peptides ...............................................................11 G. Instructions for Analytical Services .........................................................12 H. X-Ray Diffraction Analysis of Compounds .............................................13 I. Nicotine Research Cigarettes Drug Supply Program .............................16 J. Ordering Guidelines for Nicotine Research Cigarettes (NRCs)..............18 K. Ordering Guidelines for Marijuana and Marijuana Cigarettes ................21 L. Important Addresses, Telephone & Fax Numbers ..................................24 M. Available Drugs, Compounds, and Dosage Forms ..............................25
    [Show full text]
  • List of Narcotic Substances Circulation of Which Is Restricted in Uzbekistan
    List of narcotic substances circulation of which is restricted in Uzbekistan 1. 2C-B(4-bromo-2,5-dimethoxyphenethylamine) 2. 3-methylfentanyl 3. 3-methylthiofentanyl 4. 3-Monoacetylmorphine 5. 4-methylaminorex 6. 6- Monoacetylmorphine 7. Acetorphine 8. Acetyl Dihydrocodeine 9. Acetyl-alfametilfentanil 10. Acetylated opium 11. Acetylcodeine 12. Acetylmethadol 13. Alfametadol 14. Alfatsetilmetadol 15. all fungi that contain Psilocine and Psilocybine 16. Allylprodine 17. Alpha Methylfentanyl 18. Alpha Metiltiofentanil 19. Alphaprodine 20. Anileridin 21. Benzethidine 22. Benzylmorphine 23. Betacetylmethadol 24. Betahydroxyfentanyl 25. Betameprodine 26. Betamethadol 27. Betaprodine 28. Bezitramide 29. Cannabis oil (hashish oil) 30. Cannabis, marihuana 31. Cathine ((+)-norpseudoephedrine) 32. Cathinone (l-alpha-aminopropiofenon) 33. Clonitazene 34. Cocaine 35. Codoxime 36. d- Methadone 37. DB [L-(3,4 - methylenedioxyphenyl) -2 butanamine] 38. Desmethylprodine; MPPP (1-methyl-4-phenyl-4-propionoxypiperidine) 39. Desomorphine 40. DET (N,N-diethyltryptamine) 41. Dexamphetamine 42. Diampromide 43. Diethyl phosphate 44. Diethylthiambutene 45. Dihydromorphine 46. Dimenoxadol 47. Dimepheptanol 48. Dimethylthiambutene 49. Dioxaphetyl butyrate 50. Diphenoxine 51. Dipipanone 52. DMA (2,5-dimethoxyamphetamine) 53. DMGP (dimetilgeptilpiran) 54. DMT (dimethyltryptamine) 55. DOB (d, L-2,5-dimethoxy-4-bromo-amphetamine) 56. DOC (d, L-2,5-dimethoxy-4-chloro-amphetamine) 57. DOET (2,5-dimethoxy-4-ethylamphetamine) 58. Drotebanol 59. Ecgonine 60. Ephedrone 61. Ethylmethylthiambutene 62. Eticyclidine 63. Etonitazene 64. Etorphine 65. Etoxeridine 66. Etryptamine 67. Furethidine 68. Hashish (Anasha, cannabis resin) 69. Heroin (Diacetylmorphine) 70. Hydrocodone 71. Hydrocodone phosphate 72. Hydromorphinol 73. Hydromorphone 74. Isomethadone 75. Ketobemidone 76. Khat 77. L- Methadone 78. Levomethorphan 79. Levomoramide 80. Levophenacylmorphan 81. Levorphanol 82. Lysergic acid and its preparations, that include d-Lysergide (LSD, LSD-25) 83.
    [Show full text]
  • Manchester Parkade / DON't MISS
    M - MANCHESTER HERALD, Friday. April 15. 1983 Hay looks ahead ‘Shoot to kill’ Wilcox misses to his old classes order pays off perfect game L- ... page 3 ... page 4 page 15 Cloudy today Manchester, Conn. and Sunday Saturday, April 16, 1983 Tom orrow — See page 2 illanrli^atfr Mmlb Single copy: 25$ /m\ Feldstein says m . • k f economy now J*5,SV"w ft ■ on solid footing WASHINGTON (U PI) - In a stronger than I expected.” double dose of good news for the The decline in wholesale prices nation's economic recovery, the was bigger in the January-March f government reported Friday that quarter than in any three-month wholesale prices dropped 0.1 per­ period since the end of 1952, Labor m i cent and factory production made Department economists said F ri­ April l6th a strong 1.1 percent gain in March. day. It was the first quarterly The decline in wholesale prices decline of any size since 1976. from January to March, as mea­ The recovery reached through sured by the government’s Pro­ the supply pipeline to raise raw ducer Price Index, was the steep­ materials prices by 0.6 percent. est for any quarter in more than But the combination of busier . " d three decades, the Labor Depart­ factories and declining business ment said. prices overall was especially fa- At the same time, a sharp surge vtTrable, economist Sinai said. in factory production of construc­ “ It’s a double dose of good news" tion supplies, and even furniture that typically only happens “ in the and carpeting, was triggered by a early stages of the recovery,” he housing boom that lifted Febru­ said.
    [Show full text]
  • WO 2017/066488 Al
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2017/066488 A l 2 0 April 2017 (20.04.2017) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A61K 31/485 (2006.01) A61P 25/04 (2006.01) kind of national protection available): AE, AG, AL, AM, A61K 31/5415 (2006.01) A61P 1/08 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/US20 16/0569 10 HN, HR, HU, ID, IL, EST, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 13 October 2016 (13.10.201 6) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (25) Filing Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/240,965 13 October 2015 (13. 10.2015) US (84) Designated States (unless otherwise indicated, for every 62/300,014 25 February 2016 (25.02.2016) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: CHARLESTON LABORATORIES, INC.
    [Show full text]
  • 2021 Nhl Awards Presented by Bridgestone Information Guide
    2021 NHL AWARDS PRESENTED BY BRIDGESTONE INFORMATION GUIDE TABLE OF CONTENTS 2021 NHL Award Winners and Finalists ................................................................................................................................. 3 Regular-Season Awards Art Ross Trophy ......................................................................................................................................................... 4 Bill Masterton Memorial Trophy ................................................................................................................................. 6 Calder Memorial Trophy ............................................................................................................................................. 8 Frank J. Selke Trophy .............................................................................................................................................. 14 Hart Memorial Trophy .............................................................................................................................................. 18 Jack Adams Award .................................................................................................................................................. 24 James Norris Memorial Trophy ................................................................................................................................ 28 Jim Gregory General Manager of the Year Award .................................................................................................
    [Show full text]
  • Still Measuring up the Remarkable Story of René Syler in Her Own Words
    Still Measuring Up The remarkable story of René Syler in her own words Presort Standard US Postage PAID Permit No. 161 Journal, Spring 2008Harrisonburg, | www.nabj.org VA | National Association of Black Journalists | 1 2 | National Association of Black Journalists | www.nabj.org | Journal, Spring 2008 Features 8 – Thomas Morgan III: A life remembered. 18 – Out of the Mainstream: TV One’s Cathy Hughes on race, presidential politics, and oh yeah, dominating the airwaves. 20 – Fade to white: In a revealing, personal memoir, Lee Thomas takes readers on a journey of change. 33 – Internships: Now that you have one, here is how to keep it and succeed. Africa 22 – Back to Africa: Seven NABJ members traveled to Senegal late last year to tell the stories of climate change, HIV/AIDS, disease and education. Here are their stories. 26 – Ghana: Bonnie Newman Davis, one of NABJ’s Ethel Payne Fellows explores why Ghana is everything she thought it would be. Digital Journalism Three veteran digital journalists, Andrew Humphrey, Ju-Don Marshall Rob- erts and Mara Schiavocampo, dig through the jargon to decode the digital revolution. 28 – The Future is Here 30 – Tips for Media Newbies 30 – As newsrooms change, journalists adjust Cover Story The NABJ Journal looks at the issue of breast cancer through the eyes of our members. 10 – New Year’s Resolutions: René Syler goes first person to discuss her difficult year and her prospects for the future. 15 – No fear: NBC’s Hoda Kotb gains strength in battle against cancer. 16 – Out in the open: Atlanta’s JaQuitta Williams on why it was important to share her story with others.
    [Show full text]
  • ATP, 489 Absolute Configuration Benzomotphans, 204 Levotphanol
    Index AIDA, 495 Affinity labeling, analogs of (Cont.) cAMP, 409, 489 motphine,448 ATP, 409, 489 naltrexone, 449 [3H] ATP, 489 norlevotphanol,449 Absolute configuration normetazocine, 181 benzomotphans, 204 norpethidine, 232 levotphanol, 115 oripavine, 453 methadone and analogs, 316 oxymotphone, 449 motphine, 86 K-Agonists, 179,405,434 phenoperidine, 234 Aid in Interactive Drug Analysis, 495 piperazine derivatives, 399 [L-Ala2] dermotphin, 363 prodines and analogs, 272 [D-Ala, D-Leu] enkephalin (DADL), 68, 344 sinomenine, 28, 115 [D-Ala2 , Bugs] enkephalinamide, 347, 447 viminol, 400 [D-Ala2, Met'] enkephalinamide, 337, 346, Ac 61-91,360 371,489 Acetylcholine, 5, 407 [D-Ala2]leu-enkephalin, 344, 346, 348 Acetylcholine analogs, 186, 191 [D-Ala2] met-enkephalin, 348 l-Acetylcodeine, 32 [D-Ala2] enkephalins, 347 Acetylmethadols (a and (3) Alfentanil, 296 maintenance of addicts by a-isomer, 304, 309 (±)-I1(3-Alkylbenzomotphans, 167, 170 metabolism, 309 11(3-Alkylbenzomotphans, 204 N-allyl and N-CPM analogs, 310, 431 7-Alkylisomotphinans, 146 stereochemistry, 323 N-Alkylnorketobemidones, 431 synthesis, 309 N-Alkylnorpethidines, 233 X-ray crystallography, 327 N-Allylnormetazocine, 420 6-Acetylmotphine, receptor binding, 27 N-Allylnormotphine, 405 Acetylnormethadol, 323 N-Allylnorpethidine, 233 8(3-Acyldihydrocodeinones, 52 3-Allylprodines (a and (3), 256 14-Acyl-4,5-epoxymotphinans, 58 'H-NMR and stereochemistry, 256 7-Acylhydromotphones, 128 X-ray crystallography, 256 Addiction, 4 N-Allylnormetazocine, 420 Adenylate cyclase, 6, 409, 413, 424,
    [Show full text]
  • Alcohol and Drug Abuse Subchapter 9 Regulated Drug Rule 1.0 Authority
    Chapter 8 – Alcohol and Drug Abuse Subchapter 9 Regulated Drug Rule 1.0 Authority This rule is established under the authority of 18 V.S.A. §§ 4201 and 4202 which authorizes the Vermont Board of Health to designate regulated drugs for the protection of public health and safety. 2.0 Purpose This rule designates drugs and other chemical substances that are illegal or judged to be potentially fatal or harmful for human consumption unless prescribed and dispensed by a professional licensed to prescribe or dispense them, and used in accordance with the prescription. The rule restricts the possession of certain drugs above a specified quantity. The rule also establishes benchmark unlawful dosages for certain drugs to provide a baseline for use by prosecutors to seek enhanced penalties for possession of higher quantities of the drug in accordance with multipliers found at 18 V.S.A. § 4234. 3.0 Definitions 3.1 “Analog” means one of a group of chemical components similar in structure but different with respect to elemental composition. It can differ in one or more atoms, functional groups or substructures, which are replaced with other atoms, groups or substructures. 3.2 “Benchmark Unlawful Dosage” means the quantity of a drug commonly consumed over a twenty-four hour period for any therapeutic purpose, as established by the manufacturer of the drug. Benchmark Unlawful dosage is not a medical or pharmacologic concept with any implication for medical practice. Instead, it is a legal concept established only for the purpose of calculating penalties for improper sale, possession, or dispensing of drugs pursuant to 18 V.S.A.
    [Show full text]