Detection and Characterization of a Novel Bat-Borne Coronavirus in Singapore Using Multiple Molecular Approaches
RESEARCH ARTICLE Lim et al., Journal of General Virology 2019;100:1363–1374 DOI 10.1099/jgv.0.001307 Detection and characterization of a novel bat-borne coronavirus in Singapore using multiple molecular approaches Xiao Fang Lim1,2, Chengfa Benjamin Lee3, Sarah Marie Pascoe3, Choon Beng How3, Sharon Chan3, Jun Hao Tan1, Xinglou Yang1,4, Peng Zhou4, Zhengli Shi4, October M. Sessions1,5,6, Lin-Fa Wang1, Lee Ching Ng2, Danielle E. Anderson1,* and Grace Yap2,* Abstract Bats are important reservoirs and vectors in the transmission of emerging infectious diseases. Many highly pathogenic viruses such as SARS-CoV and rabies-related lyssaviruses have crossed species barriers to infect humans and other animals. In this study we monitored the major roost sites of bats in Singapore, and performed surveillance for zoonotic pathogens in these bats. Screening of guano samples collected during the survey uncovered a bat coronavirus (Betacoronavirus) in Cynopterus brachyotis, commonly known as the lesser dog-faced fruit bat. Using a capture-enrichment sequencing platform, the full- length genome of the bat CoV was sequenced and found to be closely related to the bat coronavirus HKU9 species found in Leschenault’s rousette discovered in the Guangdong and Yunnan provinces. INTRODUctiON CoVs in general can cause disease in a variety of domestic and Infectious diseases continue to be a major threat to modern wild animals, as well as in humans, whereas alpha- and beta- society and coronaviruses (CoVs) are one of the most notable coronaviruses predominantly infect mammals. SARS-CoV virus families responsible for recent, highly pathogenic viral and MERS-CoV belong to the genus Betacoronavirus, under disease outbreaks.
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