DOCSLIB.ORG

Correlation Between Jejunal Microbial Diversity and Muscle Fatty Acids

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Correlation Between Jejunal Microbial Diversity and Muscle Fatty Acids www.nature.com/scientificreports OPEN Correlation between Jejunal Microbial Diversity and Muscle Fatty Acids Deposition in Broilers Received: 24 April 2019 Accepted: 12 July 2019 Reared at Diferent Ambient Published: xx xx xxxx Temperatures Xing Li1, Zhenhui Cao1, Yuting Yang1, Liang Chen2, Jianping Liu3, Qiuye Lin4, Yingying Qiao1, Zhiyong Zhao5, Qingcong An1, Chunyong Zhang1, Qihua Li1, Qiaoping Ji6, Hongfu Zhang2 & Hongbin Pan1 Temperature, which is an important environmental factor in broiler farming, can signifcantly infuence the deposition of fatty acids in muscle. 300 one-day-old broiler chicks were randomly divided into three groups and reared at high, medium and low temperatures (HJ, MJ and LJ), respectively. Breast muscle and jejunal chyme samples were collected and subjected to analyses of fatty acid composition and 16S rRNA gene sequencing. Through spearman’s rank correlation coefcient, the data were used to characterize the correlation between jejunal microbial diversity and muscle fatty acid deposition in the broilers. The results showed that Achromobacter, Stenotrophomonas, Pandoraea, Brevundimonas, Petrobacter and Variovorax were signifcantly enriched in the MJ group, and all of them were positively correlated with the fatty acid profling of muscle and multiple lipid metabolism signaling pathways. Lactobacillus was signifcantly enriched in the HJ group and exhibited a positive correlation with fatty acid deposition. Pyramidobacter, Dialister, Bacteroides and Selenomonas were signifcantly enriched in the LJ group and displayed negative correlation with fatty acid deposition. Taken together, this study demonstrated that the jejunal microfora manifested considerable changes at high and low ambient temperatures and that jejunal microbiota changes were correlated with fatty acid deposition of muscle in broilers. Poultry are important protein sources in human diet and hence they are of enormous economic value. Te close symbiotic relationship between the host and gut microbiota is crucial in sustaining host health1. Te gut microbi- ota help beneft host health through participating in nutrients and energy metabolism, regulating host well-being, and promoting innate and acquired immunity2–6. For example, germ-free (GF) C57BL/6J mice consuming a diet high in fat had elevated fecal lipid levels compared with conventional mice7. Conventionalized GF zebra fsh displayed greater uptake of long- and short-chain fatty acid in the intestinal epithelium under fasted and fed conditions8. In addition, decreased lymphatic lipids level was ameliorated in antibiotic-treated rats afer fat challenge9. A recent study addressed the role of the jejunal microbiome in regulating dietary fat digestion and absorption in a mouse model10. A critical role for this microbial community in transducing dietary signals to the host was illustrated to adapt its digestive and absorptive capacity to the dietary fat availability. Tese above 1Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China. 2State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. 3Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China. 4College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201, China. 5Yunnan Animal Science and Veterinary Institute, Kunming, 650201, China. 6Yunnan Mudao Biology Technology Co., Ltd., Kunming, 650201, China. Xing Li, Zhenhui Cao and Yuting Yang contributed equally. Correspondence and requests for materials should be addressed to H.Z. (email: [email protected]) or H.P. (email: [email protected]) SCIENTIFIC REPORTS | (2019) 9:11022 | https://doi.org/10.1038/s41598-019-47323-0 1 www.nature.com/scientificreports/ www.nature.com/scientificreports Item HJ MJ LJ P-value AB A B C14:0 (C14H28O2) 1.79 ± 0.90 2.43 ± 1.29 1.46 ± 1.08 0.018 C14:1 (C14H26O2) 0.58 ± 0.30 0.63 ± 0.38 0.13 ± 0.13 0.084 C15:0 (C15H30O2) 0.27 ± 0.24 0.48 ± 0.26 0.16 ± 0.17 0.766 AB A B C16:0 (C16H32O2) 67.03 ± 24.62 127.37 ± 72.59 54.63 ± 18.58 0.011 A A B C16:1 (C16H30O2) 19.83 ± 12.70 25.19 ± 17.89 7.78 ± 3.74 0.002 b ab a C17:0 (C17H34O2) 0.09 ± 0.21 0.74 ± 0.42 0.27 ± 0.28 0.021 b a b C18:0 (C18H36O2) 26.17 ± 12.15 45.10 ± 22.64 29.86 ± 15.09 0.019 ab a b C18:ln9c (C18H34O2) 101.32 ± 66.10 149.39 ± 100.93 64.16 ± 23.32 0.059 b a b C18:2n6c (C18H32O2) 48.13 ± 15.90 96.86 ± 55.11 46.74 ± 17.96 0.02 C20:0 (C20H40O2) 0.21 ± 0.28 0.40 ± 0.35 0.26 ± 0.22 0.057 b a b C18:3n6 (C18H30O2) 0.83 ± 0.65 1.20 ± 0.62 0.44 ± 0.39 0.033 AB A B C20:1 (C20H38O2) 2.74 ± 1.72 3.73 ± 2.36 1.53 ± 0.61 0.01 ab a b C18:3n3 (C18H30O2) 1.04 ± 0.41 1.55 ± 0.85 0.85 ± 0.23 0.047 Bb Aa Bc C20:2 (C20H36O2) 1.45 ± 0.53 2.42 ± 1.03 1.08 ± 0.34 <0.001 B A B C20:3n6 (C20H34O2) 3.63 ± 0.99 5.93 ± 1.99 2.85 ± 0.78 <0.001 B A B C20:3n3 (C20H36O6) 10.32 ± 2.48 22.73 ± 8.91 10.42 ± 2.50 <0.001 B A B C22:6n3 (C22H32O2) 1.21 ± 1.18 3.55 ± 1.46 2.10 ± 0.68 0.006 Total SFA 114.92 ± 63.91 176.54 ± 96.77 105.64 ± 70.82 0.17 Total PUFA 64.35 ± 20.38B 134.86 ± 67.68A 64.11 ± 23.03B 0.003 Total MUFA 125.28 ± 83.03ab 178.82 ± 121.67a 73.62 ± 27.32b 0.044 PUFA:SFA ratio 0.71 ± 0.03 0.79 ± 0.05 0.76 ± 0.10 0.056 n-3 PUFA 12.80 ± 4.24B 27.92 ± 11.10A 13.45 ± 3.91B <0.001 n-6 PUFA 52.03 ± 17.03B 103.99 ± 57.60A 49.83 ± 18.72B 0.003 n-6:n-3 ratio 4.70 ± 0.80 3.58 ± 1.00 4.67 ± 2.01 0.14 Table 1. Efects of High and Low Ambient Temperatures on Fatty Acids in Broilers Muscle. A,BMeans in the same row with diferent superscripts are signifcantly diferent. (P < 0.01); a,bMeans in the same row with diferent superscripts are signifcantly diferent. (P < 0.05); n = 8 per treatment group; SFA - saturated fatty acid, PUFA - polyunsaturated fatty acid, MUFA - monounsaturated fatty acid. fndings demonstrated that the microbiome in jejunum may be a highly relevant target in our eforts to solve the problems associated with fat deposition. Environment and management related stresses become more severe under intensive poultry farming condi- tions where selection of birds with higher production performance and better feed conversion efciency makes them more vulnerable to heat stress and necessitates careful management and preventive steps to decrease pos- sible loss11,12. Signifcant shifs in proportions of fecal microbial population were seen on mice when maintained under the cold environment with higher ratio of Firmicutes/Bacteroidetes13. Tese studies suggest that changes in gut microbiome diversity and/or richness at varied environment temperatures have shown to be associated with fat acid deposition. However, little is known about underlying mechanism that gut microbiota regulate the fatty acid metabolism in broilers at diferent environmental temperatures. Tus, this study aims to explore the links of microbial diversity and fatty acid composition in broilers raised at diferent ambient temperatures, thereby providing experimental evidence to understand on how environmental temperatures afect the health and growth performance of broilers via modulation of intestinal microbiota. Results Fatty acid deposition in breast muscle. As shown in Table 1, the contents of polyunsaturated fatty acid (PUFAs), n-3 PUFAs, n-6 PUFAs, C18:0, C18:2n6c, C18:3n6, C20:2, C20:3n6, C20:3n3 and C22:6n3 in breast muscle of the MJ group were signifcantly higher (P < 0.05) than those in both HJ and LJ groups. Lower mono- unsaturated fatty acids (MUFA), C16:1, C16:1, C18:ln9c, C20:1 and C18:3n3 of breast muscle in LJ group were observed (P < 0.05) compared with the MJ group. As such, both high and low temperature treatments afected fatty acid composition in breast muscle. Analysis of microbial diversity. In total, 1,926,570 clean reads were generated from the 36 jejunal sam- ples, which yielded an average of 53,515 clean reads (35,237–64,441) per sample and an average length of 422 bp clean reads. To minimize the efect of the sequencing depth on the measurement of microbial compositions, the library size was rarefed to 32,296 reads per sample using the QIIME pipeline (Supplementary Table S3). With the sequence similarity of at least 97%, 943, 885 and 1123 OTUs were obtained from HJ, MJ and LJ groups and the proportion of core microbiota in the jejunal contents of broilers was 50%, 53% and 42%, respectively (Fig. 1). Te diversity indices were calculated based on the OTUs of each library (Supplementary Table S4). As shown in Fig. 2, the index of chao1, observed species and PD whole tree in the LJ group were signifcantly higher (P < 0.05) than those in the MJ group; the LJ group had the highest indices of Shannon and Simpson (P < 0.05). In comparison, there were no signifcant diferences in diversity indicators between the HJ group and MJ or LJ group except that SCIENTIFIC REPORTS | (2019) 9:11022 | https://doi.org/10.1038/s41598-019-47323-0 2 www.nature.com/scientificreports/ www.nature.com/scientificreports Figure 1.
Load more

Recommended publications
  • Community Analysis of Microbial Sharing and Specialization in A
    Downloaded from http://rspb.royalsocietypublishing.org/ on March 15, 2017 Community analysis of microbial sharing rspb.royalsocietypublishing.org and specialization in a Costa Rican ant–plant–hemipteran symbiosis Elizabeth G. Pringle1,2 and Corrie S. Moreau3 Research 1Department of Biology, Program in Ecology, Evolution, and Conservation Biology, University of Nevada, Cite this article: Pringle EG, Moreau CS. 2017 Reno, NV 89557, USA 2Michigan Society of Fellows, University of Michigan, Ann Arbor, MI 48109, USA Community analysis of microbial sharing and 3Department of Science and Education, Field Museum of Natural History, 1400 South Lake Shore Drive, specialization in a Costa Rican ant–plant– Chicago, IL 60605, USA hemipteran symbiosis. Proc. R. Soc. B 284: EGP, 0000-0002-4398-9272 20162770. http://dx.doi.org/10.1098/rspb.2016.2770 Ants have long been renowned for their intimate mutualisms with tropho- bionts and plants and more recently appreciated for their widespread and diverse interactions with microbes. An open question in symbiosis research is the extent to which environmental influence, including the exchange of Received: 14 December 2016 microbes between interacting macroorganisms, affects the composition and Accepted: 17 January 2017 function of symbiotic microbial communities. Here we approached this ques- tion by investigating symbiosis within symbiosis. Ant–plant–hemipteran symbioses are hallmarks of tropical ecosystems that produce persistent close contact among the macroorganism partners, which then have substantial opportunity to exchange symbiotic microbes. We used metabarcoding and Subject Category: quantitative PCR to examine community structure of both bacteria and Ecology fungi in a Neotropical ant–plant–scale-insect symbiosis. Both phloem-feed- ing scale insects and honeydew-feeding ants make use of microbial Subject Areas: symbionts to subsist on phloem-derived diets of suboptimal nutritional qual- ecology, evolution, microbiology ity.
    [Show full text]
  • Microbiology of Endodontic Infections
    Scient Open Journal of Dental and Oral Health Access Exploring the World of Science ISSN: 2369-4475 Short Communication Microbiology of Endodontic Infections This article was published in the following Scient Open Access Journal: Journal of Dental and Oral Health Received August 30, 2016; Accepted September 05, 2016; Published September 12, 2016 Harpreet Singh* Abstract Department of Conservative Dentistry & Endodontics, Gian Sagar Dental College, Patiala, Punjab, India Root canal system acts as a ‘privileged sanctuary’ for the growth and survival of endodontic microbiota. This is attributed to the special environment which the microbes get inside the root canals and several other associated factors. Although a variety of microbes have been isolated from the root canal system, bacteria are the most common ones found to be associated with Endodontic infections. This article gives an in-depth view of the microbiology involved in endodontic infections during its different stages. Keywords: Bacteria, Endodontic, Infection, Microbiology Introduction Microorganisms play an unequivocal role in infecting root canal system. Endodontic infections are different from the other oral infections in the fact that they occur in an environment which is closed to begin with since the root canal system is an enclosed one, surrounded by hard tissues all around [1,2]. Most of the diseases of dental pulp and periradicular tissues are associated with microorganisms [3]. Endodontic infections occur and progress when the root canal system gets exposed to the oral environment by one reason or the other and simultaneously when there is fall in the body’s immune when the ingress is from a carious lesion or a traumatic injury to the coronal tooth structure.response [4].However, To begin the with, issue the if notmicrobes taken arecare confined of, ultimately to the leadsintra-radicular to the egress region of pathogensIn total, and bacteria their by-productsdetected from from the the oral apical cavity foramen fall into to 13 the separate periradicular phyla, tissues.
    [Show full text]
  • Desulfuribacillus Alkaliarsenatis Gen. Nov. Sp. Nov., a Deep-Lineage
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Extremophiles (2012) 16:597–605 DOI 10.1007/s00792-012-0459-7 ORIGINAL PAPER Desulfuribacillus alkaliarsenatis gen. nov. sp. nov., a deep-lineage, obligately anaerobic, dissimilatory sulfur and arsenate-reducing, haloalkaliphilic representative of the order Bacillales from soda lakes D. Y. Sorokin • T. P. Tourova • M. V. Sukhacheva • G. Muyzer Received: 10 February 2012 / Accepted: 3 May 2012 / Published online: 24 May 2012 Ó The Author(s) 2012. This article is published with open access at Springerlink.com Abstract An anaerobic enrichment culture inoculated possible within a pH range from 9 to 10.5 (optimum at pH with a sample of sediments from soda lakes of the Kulunda 10) and a salt concentration at pH 10 from 0.2 to 2 M total Steppe with elemental sulfur as electron acceptor and for- Na? (optimum at 0.6 M). According to the phylogenetic mate as electron donor at pH 10 and moderate salinity analysis, strain AHT28 represents a deep independent inoculated with sediments from soda lakes in Kulunda lineage within the order Bacillales with a maximum of Steppe (Altai, Russia) resulted in the domination of a 90 % 16S rRNA gene similarity to its closest cultured Gram-positive, spore-forming bacterium strain AHT28. representatives. On the basis of its distinct phenotype and The isolate is an obligate anaerobe capable of respiratory phylogeny, the novel haloalkaliphilic anaerobe is suggested growth using elemental sulfur, thiosulfate (incomplete as a new genus and species, Desulfuribacillus alkaliar- T T reduction) and arsenate as electron acceptor with H2, for- senatis (type strain AHT28 = DSM24608 = UNIQEM mate, pyruvate and lactate as electron donor.
    [Show full text]
  • Reclassification of Agrobacterium Ferrugineum LMG 128 As Hoeflea
    International Journal of Systematic and Evolutionary Microbiology (2005), 55, 1163–1166 DOI 10.1099/ijs.0.63291-0 Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Alvaro Peix,1 Rau´l Rivas,2 Martha E. Trujillo,2 Marc Vancanneyt,3 Encarna Vela´zquez2 and Anne Willems3 Correspondence 1Departamento de Produccio´n Vegetal, Instituto de Recursos Naturales y Agrobiologı´a, Encarna Vela´zquez IRNA-CSIC, Spain [email protected] 2Departamento de Microbiologı´a y Gene´tica, Lab. 209, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain 3Laboratory of Microbiology, Dept Biochemistry, Physiology and Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium Members of the species Agrobacterium ferrugineum were isolated from marine environments. The type strain of this species (=LMG 22047T=ATCC 25652T) was recently reclassified in the new genus Pseudorhodobacter, in the order ‘Rhodobacterales’ of the class ‘Alphaproteobacteria’. Strain LMG 128 (=ATCC 25654) was also initially classified as belonging to the species Agrobacterium ferrugineum; however, the nearly complete 16S rRNA gene sequence of this strain indicated that it does not belong within the genus Agrobacterium or within the genus Pseudorhodobacter. The closest related organism, with 95?5 % 16S rRNA gene similarity, was Aquamicrobium defluvii from the family ‘Phyllobacteriaceae’ in the order ‘Rhizobiales’. The remaining genera from this order had 16S rRNA gene sequence similarities that were lower than 95?1 % with respect to strain LMG 128. These phylogenetic distances suggested that strain LMG 128 belonged to a different genus. The major fatty acid present in strain LMG 128 was mono-unsaturated straight chain 18 : 1v7c.
    [Show full text]
  • Natronobacillus Azotifigens Gen. Nov., Sp. Nov., an Anaerobic Diazotrophic
    Extremophiles (2008) 12:819–827 DOI 10.1007/s00792-008-0188-0 ORIGINAL PAPER Natronobacillus azotifigens gen. nov., sp. nov., an anaerobic diazotrophic haloalkaliphile from soda-rich habitats I. D. Sorokin Æ E. V. Zadorina Æ I. K. Kravchenko Æ E. S. Boulygina Æ T. P. Tourova Æ D. Y. Sorokin Received: 27 June 2008 / Accepted: 17 August 2008 / Published online: 4 September 2008 Ó The Author(s) 2008. This article is published with open access at Springerlink.com Abstract Gram-positive bacteria capable of nitrogen obligately alkaliphilic and highly salt-tolerant natrono- fixation were obtained in microoxic enrichments from soda philes (chloride-independent sodaphiles). Growth was soils in south-western Siberia, north-eastern Mongolia, and possible within a pH range from 7.5 to 10.6, with an the Lybian desert (Egypt). The same organisms were optimum at 9.5–10, and within a salt range from 0.2 to 4 M obtained in anoxic enrichments with glucose from soda Na?, with an optimum at 0.5–1.5 M for the different lake sediments in the Kulunda Steppe (Altai, Russia) using strains. The nitrogenase activity in the whole cells also had nitrogen-free alkaline medium of pH 10. The isolates were an alkaline pH optimum but was much more sensitive to represented by thin motile rods forming terminal round high salt concentrations compared to the growing cells. The endospores. They are strictly fermentative saccharolytic isolates formed a compact genetic group with a high level anaerobes but tolerate high oxygen concentrations, proba- of DNA similarity. Phylogenetic analysis based on 16S- bly due to a high catalase activity.
    [Show full text]
  • Extended Evaluation of Viral Diversity in Lake Baikal Through Metagenomics
    microorganisms Article Extended Evaluation of Viral Diversity in Lake Baikal through Metagenomics Tatyana V. Butina 1,* , Yurij S. Bukin 1,*, Ivan S. Petrushin 1 , Alexey E. Tupikin 2, Marsel R. Kabilov 2 and Sergey I. Belikov 1 1 Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Ulan-Batorskaya Str., 3, 664033 Irkutsk, Russia; [email protected] (I.S.P.); [email protected] (S.I.B.) 2 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090 Novosibirsk, Russia; [email protected] (A.E.T.); [email protected] (M.R.K.) * Correspondence: [email protected] (T.V.B.); [email protected] (Y.S.B.) Abstract: Lake Baikal is a unique oligotrophic freshwater lake with unusually cold conditions and amazing biological diversity. Studies of the lake’s viral communities have begun recently, and their full diversity is not elucidated yet. Here, we performed DNA viral metagenomic analysis on integral samples from four different deep-water and shallow stations of the southern and central basins of the lake. There was a strict distinction of viral communities in areas with different environmental conditions. Comparative analysis with other freshwater lakes revealed the highest similarity of Baikal viromes with those of the Asian lakes Soyang and Biwa. Analysis of new data, together with previ- ously published data allowed us to get a deeper insight into the diversity and functional potential of Baikal viruses; however, the true diversity of Baikal viruses in the lake ecosystem remains still un- Citation: Butina, T.V.; Bukin, Y.S.; Petrushin, I.S.; Tupikin, A.E.; Kabilov, known.
    [Show full text]
  • Metaproteomics Characterization of the Alphaproteobacteria
    Avian Pathology ISSN: 0307-9457 (Print) 1465-3338 (Online) Journal homepage: https://www.tandfonline.com/loi/cavp20 Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar To cite this article: José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar (2019) Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssusgallinae (De Geer, 1778), Avian Pathology, 48:sup1, S52-S59, DOI: 10.1080/03079457.2019.1635679 To link to this article: https://doi.org/10.1080/03079457.2019.1635679 © 2019 The Author(s). Published by Informa View supplementary material UK Limited, trading as Taylor & Francis Group Accepted author version posted online: 03 Submit your article to this journal Jul 2019. Published online: 02 Aug 2019. Article views: 694 View related articles View Crossmark data Citing articles: 3 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=cavp20 AVIAN PATHOLOGY 2019, VOL. 48, NO. S1, S52–S59 https://doi.org/10.1080/03079457.2019.1635679 ORIGINAL ARTICLE Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero a,b, Sandra Díaz-Sanchez a, Olivier Sparagano c, Robert D. Finn d, José de la Fuente a,e and Margarita Villar a aSaBio.
    [Show full text]
  • Taxonomical and Physiological Comparisons of the Three Species of the Genus Amphibacillus
    J. Gen. Appl. Microbiol., 55, 155‒162 (2009) Full Paper Taxonomical and physiological comparisons of the three species of the genus Amphibacillus Toshiaki Arai,1,† Shuhei Yanahashi,1,† Junichi Sato,1 Takumi Sato,1 Morio Ishikawa,2 Yukimichi Koizumi,2 Shinji Kawasaki,1 Youichi Niimura,1,* and Junichi Nakagawa3 1 Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156‒8502, Japan 2 Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156‒8502, Japan 3 Department of Food Science and Technology, Tokyo University of Agriculture, Abashiri, Hokkaido 099‒2493, Japan (Received December 2, 2008; Accepted December 22, 2008) Amphibacillus is a genus for Gram-positive, spore-forming, rod-shaped, facultatively anaerobic bacteria with low-G+C content of DNA, established by Niimura et al. in 1990. Amphibacillus xy- lanus, the type species of the genus, grows well under both strictly anaerobic and aerobic condi- tions in spite of lacking any isoprenoid quinones, cytochromes, and catalase. Amphibacillus fermentum and Amphibacillus tropicus were later proposed by Zhilina et al. in 2001 for the iso- lates from a soda lake. In this paper, we revealed the latter two species also lacked isoprenoid quinones, cytochrome and catalase, and that they grew well under strictly anaerobic and aerobic conditions. The consistent growth of A. xylanus under both conditions is due to the presence of anaerobic and aerobic pathways for glucose metabolism in the organism. Although A. fermen- tum and A. tropicus are supposed to have a side enzymatic pyruvate pathway to produce lactate under both conditions, the two species have two major pyruvate metabolic pathways as ob- served in A.
    [Show full text]
  • Thermolongibacillus Cihan Et Al
    Genus Firmicutes/Bacilli/Bacillales/Bacillaceae/ Thermolongibacillus Cihan et al. (2014)VP .......................................................................................................................................................................................... Arzu Coleri Cihan, Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey Kivanc Bilecen and Cumhur Cokmus, Department of Molecular Biology & Genetics, Faculty of Agriculture & Natural Sciences, Konya Food & Agriculture University, Konya, Turkey Ther.mo.lon.gi.ba.cil’lus. Gr. adj. thermos hot; L. adj. Type species: Thermolongibacillus altinsuensis E265T, longus long; L. dim. n. bacillus small rod; N.L. masc. n. DSM 24979T, NCIMB 14850T Cihan et al. (2014)VP. .................................................................................. Thermolongibacillus long thermophilic rod. Thermolongibacillus is a genus in the phylum Fir- Gram-positive, motile rods, occurring singly, in pairs, or micutes,classBacilli, order Bacillales, and the family in long straight or slightly curved chains. Moderate alka- Bacillaceae. There are two species in the genus Thermo- lophile, growing in a pH range of 5.0–11.0; thermophile, longibacillus, T. altinsuensis and T. kozakliensis, isolated growing in a temperature range of 40–70∘C; halophile, from sediment and soil samples in different ther- tolerating up to 5.0% (w/v) NaCl. Catalase-weakly positive, mal hot springs, respectively. Members of this genus chemoorganotroph, grow aerobically, but not under anaer- are thermophilic (40–70∘C), halophilic (0–5.0% obic conditions. Young cells are 0.6–1.1 μm in width and NaCl), alkalophilic (pH 5.0–11.0), endospore form- 3.0–8.0 μm in length; cells in stationary and death phases ing, Gram-positive, aerobic, motile, straight rods. are 0.6–1.2 μm in width and 9.0–35.0 μm in length.
    [Show full text]
  • The Effects of Ionizing Radiation on Gut Microbiota, a Systematic Review
    nutrients Systematic Review The Effects of Ionizing Radiation on Gut Microbiota, a Systematic Review Ana Fernandes 1,* , Ana Oliveira 1, Raquel Soares 2,3 and Pedro Barata 3,4,5 1 Department of Nuclear Medicine, Centro Hospitalar Universitário de São João, E.P.E., 4200-319 Porto, Portugal; [email protected] 2 Department of Biomedicine, Faculdade de Medicina da Universidade do Porto, 4200-319 Porto, Portugal; [email protected] 3 i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal; [email protected] 4 Department of Pharmaceutical Science, Faculdade de Ciências da Saúde da Universidade Fernando Pessoa, 4249-004 Porto, Portugal 5 Department of Pathology, Centro Hospitalar Universitário do Porto, 4099-001 Porto, Portugal * Correspondence: [email protected]; Tel.: +351-961312756 Abstract: Background: The human gut microbiota is defined as the microorganisms that collectively inhabit the intestinal tract. Its composition is relatively stable; however, an imbalance can be pre- cipitated by various factors and is known to be associated with various diseases. Humans are daily exposed to ionizing radiation from ambient and medical procedures, and gastrointestinal side effects are not rare. Methods: A systematic search of PubMed, EMBASE, and Cochrane Library databases was conducted. Primary outcomes were changes in composition, richness, and diversity of the gut microbiota after ionizing radiation exposure. Standard methodological procedures expected by Cochrane were used. Results: A total of 2929 nonduplicated records were identified, and based on the inclusion criteria, 11 studies were considered. Studies were heterogeneous, with differences in Citation: Fernandes, A.; Oliveira, A.; population and outcomes.
    [Show full text]
  • Isolation and Diversity of Sediment Bacteria in The
    bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Isolation and Diversity of Sediment Bacteria in the 2 Hypersaline Aiding Lake, China 3 4 Tong-Wei Guan, Yi-Jin Lin, Meng-Ying Ou, Ke-Bao Chen 5 6 7 Institute of Microbiology, Xihua University, Chengdu 610039, P. R. China. 8 9 Author for correspondence: 10 Tong-Wei Guan 11 Tel/Fax: +86 028 87720552 12 E-mail: [email protected] 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 bioRxiv preprint doi: https://doi.org/10.1101/638304; this version posted May 14, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 29 Abstract A total of 343 bacteria from sediment samples of Aiding Lake, China, were isolated using 30 nine different media with 5% or 15% (w/v) NaCl. The number of species and genera of bacteria recovered 31 from the different media significantly varied, indicating the need to optimize the isolation conditions. 32 The results showed an unexpected level of bacterial diversity, with four phyla (Firmicutes, 33 Actinobacteria, Proteobacteria, and Rhodothermaeota), fourteen orders (Actinopolysporales, 34 Alteromonadales, Bacillales, Balneolales, Chromatiales, Glycomycetales, Jiangellales, Micrococcales, 35 Micromonosporales, Oceanospirillales, Pseudonocardiales, Rhizobiales, Streptomycetales, and 36 Streptosporangiales), including 17 families, 41 genera, and 71 species.
    [Show full text]
  • Oryzicola Mucosus Gen. Nov., Sp. Nov., a Novel Slime Producing Bacterium Belonging to the Family Phyllobacteriaceae Isolated from the Rhizosphere of Rice Plants
    Oryzicola mucosus gen. nov., sp. nov., a Novel Slime Producing Bacterium Belonging to the Family Phyllobacteriaceae Isolated From the Rhizosphere of Rice Plants Geeta Chhetri Dongguk University-Seoul Jiyoun Kim Dongguk University-Seoul Inhyup Kim Dongguk University-Seoul Minchung Kang Dongguk University-Seoul Yoonseop So Dongguk University-Seoul Taegun Seo ( [email protected] ) Dongguk Univesity https://orcid.org/0000-0001-9701-2806 Research Article Keywords: Oryzicola mucosus, Phytohormone, Indole acetic acid, slime, carotenoid Posted Date: June 3rd, 2021 DOI: https://doi.org/10.21203/rs.3.rs-489985/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/18 Abstract A novel Gram-stain negative, asporogenous, slimy, rod-shaped, non-motile bacterium was isolated from the root samples collected from rice eld located in Ilsan, South Korea. Phylogenetic analysis of the 16S rRNA sequence of the bacterium revealed a close proximity to Tianweitania sediminis Z8T (96.5%) followed by genera Mesorhizobium (96.4-95.6%), Aquabacterium (95.9-95.7%), Rhizobium (95.8%) and Ochrobactrum (95.6%). Strain ROOL2T produced white slime on R2A agar plates and grew optimally at 30℃ in the presence of 1-6% (w/v) NaCl and at pH 7.5. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acids were C18 :1ω7c, summed feature 4 (comprising iso-C17:1 I and/or anteiso-C17:1 B) and summed feature 8 (comprising C18:1ω6c and/or C18:1ω7c). The polar lipid prole consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylglycerol, one unidentied aminolipid and two unidentied lipids.
    [Show full text]