CURRIULUM VITAE Of Kejin Hu PERSONAL INFORMATION

Name: Kejin HU Visa status: USA citizen Language(s): English, Chinese Home city: Vestavia Hills, AL, 35226 RANK/TITLE, Assistant Professor Department: and Molecular Genetics Division: UAB Stem Institute Business Address: SHEL 705, 1825 University Boulevard, Birmingham, AL, 35294 Phone: 205-934-4700 (office); 205-876-8693 (home); 205-703-6688 (cell) Fax: 205-975-3335

MEMBERSHIP:

ISSCR (international Society for Research), since 2011 Member of Genetics Society of America (GSA, since 2006)

EDUCATIONS  June, 1999 to May, 2003, PhD, in marine molecular at the Department of Zoology, The University of Hong Kong, Hong Kong, China.  July, 1995-October, 1997, MPhil, in fungal biochemistry/microbiology in the Hong Kong Polytechnic University.  September, 1981-July, 1985, BSc in botany/agronomy at The Central China (Huazhong) Agricultural University, Wuhan, China.

TEACHING EXPERIENCE: Advanced Stem Cell/Regenerative medicine, GBSC 709, Since 2014

SCIENTIFIC ACTIVITIES Ad hoc reviewer for the following journals: 1) Stem Cells; 2) Stem Cells and Development; 3) Stem Cell International; 4) Human ; 5) Molecular Biotechnology; 6) Comparative Biochemistry and Physiology; 7) Journal of Heredity; 8) Scientific Reports; 9) Cellular Reprogramming; 10) Cell and Tissue Research; 11) Science Bulletin; 12) Reproduction, Fertility and Development.

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Grant Reviewer for 1) Medical Research Council (MRC) of the United Kingdom (remote review, 2015); 2) New York Stem Cell Science (panel meeting from 09/28-09/30, 2016); 3) UAB internal grants

SCIENTIFIC/ACADEMIC EXPERIENCE  2011 to present, Assistant Professor, Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL  September, 2007 to July, 2011, Research Associate in human iPSC reprogramming and human pluripotent stem , and their differentiation into blood lineage, Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI.  November, 2006 to September 2007, research associate in molecular pharmacology (drug metabolism in bone marrow) at the Pharmacology Department, University of Wisconsin (Madison), Wisconsin, USA.  June, 2004 to October, 2006, Postdoctoral associate in yeast genetics and molecular biology (studying TOR signaling pathway), at Pharmacology Department of University of Pittsburgh, Pittsburgh, PA, USA.  July, 2003 to May, 2004, Postdoctoral associate in developmental biology (muscle development) of c. elegans, at , NY, USA.  March, 1998- September 1998, teaching assistant at Zoology department, Hong Kong University.  December, 1993-July, 1995, Lecturer at Hubei Academy of Agricultural Sciences, Wuhan, China.  July, 1985-December, 1993, Assistant lecturer at Hubei Academy of Agricultural Sciences.

Book chapters

1. Kejin Hu, Igor Slukvin, (2013) Pluripotent stem cells induction from umbilical cord blood. Encyclopedia of Molecular Cell Biology and Molecular Medicine, Wiley-Blackwell. 2. Kejin Hu, Igor Slukvin, (2013) Kejin Hu, Igor Slukvin, 2013, Generation of transgene-free iPSC lines form Human normal and Neoplastic Blood cells using episomal vectors. Methods in Molecular Biology, Vol. 997, Chapter 13, pp 163-176. 3. Kejin Hu. 2008. Homologous recombination and innocuous intron elimination. pp323-334. Chapter 14, In Genetic Recombination Research Progress, edited by Jacob H. Schulz.

Journal Papers (*, corresponding author)

1. Z Shao, C Yao, A Khodadadi-Jamayran, W Xu, T Townes, Michael Crowley, Kejin Hu* (2016) Reprogramming by de-bookmarking somatic transcriptional program through targeting of the BET bromodomains. Cell Reports, 16:3138-3145.

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2. Z Shao, R Zhang, A Khodadadi-Jamayran, B Chen, MR Crowley, MA Festok, DK Crossman, T Townes, Kejin Hu* (2016) The Acetyllysine Reader BRD3R Promotes Human Nuclear Reprogramming and Regulates Mitosis. Nature Communications. 7:10869. 3. L Kang, C Yao, A Khodadadi-Jamayran, W Xu, R Zhang, N Banerjee, C Chang, L Chow, T Townes, Kejin Hu* (2016) The universal 3D3 antibody of human PODXL is pluripotent cytotoxic, and identifies a residual population after extended differentiation of pluripotent stem cells. Stem Cells Dev. 25(7):556-568. 4. Kejin Hu, S Guo, G Yan, W Yuan, Y Zheng, and Y Jiang*, (2016) Ubiquitin regulates TORC1 in yeast , Molecular Microbiology 100(2):303-314. DOI: 10.1111/mmi.13319. 5. K. Suknuntha, Y. Ishii, L. Tao, Kejin HU, BE McIntosh, D. Yang, s. Swanson, R. Stewart, J Wang, J. Thomson, II Slukvin (2015) Discovery of survival factor for primitive chronic myeloid leukemia cells using induced pluripotent stem cells. Stem Cell Research. 15(3), 678-693. 6. Kejin Hu* (2014) All roads lead to induced pluripotent stem cells: the technologies of iPSC generation. Stem Cells and Development, 23(12) 1285-1300. PMCID, PMC4046204 7. Kejin Hu* (2014) Vectorology and factor delivery system in induced pluripotent stem cell reprogramming. Stem Cells and Development, 23(12): 1301-1315. PMCID, PMC4046209 8. Kejin Hu, Junying Y, James Thomson, Igor Slukvin (2011) Efficient reprogramming of mononuclear cells from archived normal and neoplastic human bone marrow and cord blood with non-integrating episomal constructs. BLOOD. Vol 117 (14), e109-e119. doi: 10.1182/blood-2010-07-298331. 9. Junying Yu, Kejin Hu, Kim Smuga-Otto, Shulan Tian, Ron Stewart, Igor I Slukvin, James A Thomson, (2009) Human induced pluripotent stem cells free of vector and transgene sequences. Science, Vol. 324, 797-801. PMCID: PMC2758053. 10. Nirav M. Amin, Kejin Hu, David Pruyne, Dino Terzic, Anthony Bretscher and Jun Liu. (2007) A Zn-finger/FH2-domain containing protein, FOZI-1, acts redundantly with CeMyoD to specify striated body wall muscle fates in the Caenorhabditis elegans postembryonic mesoderm. Development, 134(1), 19-29. 11. Kejin Hu*, 2006. Intron exclusion and the mystery of intron loss. FEBS Letters, 580(27), 6361-6365. 12. Kejin Hu* and Pak Chow Leung (2007) Food digestion by cathepsin L and the digestion-related cell differentiation in shrimp hepatopancreas. Comparative Biochemistry and Physiology Part B, 146, 69-80. 13. Kejin Hu* and Pak Chow Leung (2006) Complete, precise and innocuous loss of multiple introns in the currently intronless, active cathepsin L-like genes, and inference from this event. Molecular Phylogenetics and Evolution, 38, 685-696. 14. Ke-jin Hu, Pak-Chow Leung (2004) Shrimp cathepsin L encoded by an intronless gene has predominant expression in hepatopancreas, and occurs in the nucleus of oocyte. Comparative Biochemistry and Physiology, Part B, 137(1), 21-33.

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15. Kejin Hu*, Kwok-Wing Yeung*, Jinlian Hu, Kwok-ping Ho (2004) Screening of fungi for chitosan producers, and copper adsorption capacity of fungal chitosan and chitosanaceous materials. Carbohydrate Polymers, 58 (1): 45-52. (I am one of the two correspondence authors). 16. KeJin Hu, Kwok-Wing Yeung, Kwok-Ping Ho, and Jin-lian Hu (1999) Rapid extraction of high-quality chitosan from mycelia of Absidia glauca, Journal of Food Biochemistry, 23, 187-196. 17. Qiang Zhou and Kejin Hu (1999) Photoperiod-sensitive genic male-sterile facultative apomixis gene may be used for fixation of heterosis of hybrid rice. Hubei Agricultural Science. 1999(3): 14-15. 18. Kejin Hu* (1995) Mathematical Modeling of the cultivation of Late Keng rice in Hubei, Journal of Central China Agricultural University, Sup. Sum 20, 37-41. 19. Kejin Hu* (1990) Applying LP to planting scheme in Qifang Township. System Sciences and Comprehensive Studies in Agricultures, 1990 (3), 31-34. 20. Shizhao Li, Kejin Hu and Depei Wang (1988) Preliminary studies on the effect of Chinese medical herbs on the shelf life of mung bean cake. Hubei Agricultural Sciences, 1988(6): 41-43.

Conference presentations/posters 1. Kran Suknuntha, Yuki Ishii, Kejin Hu, James Thomson, Jean Wang, Slukvin, Igor (June 12-15, 2013) An induced pluripotent stem cell-based platform for in vitro study of chronic myeloid leukemia stem cell development and drug resistance, ISSCR annual 11th meeting. Boston, Massachusetts, USA. 2. Jeffrey Huo, Tea Soon Park, Kurinji Pandiyan , C.C. Talbot, Karan Verma, Ludovic Zimmerlin, Ann Peters, Kejin Hu, Igor Slukvin, Renee Reijo-Pera, Stephen Baylin, & Elias Zambidis (June 12-15, 2013) Rapid erasure of donor-specific epigenetic memory in blood-derived episomal hipsc augments hematopoietic potency, ISSCR annual 11th meeting. Boston, Massachusetts, USA. 3. Tea Soon Park, Ludovic Zimmerlin, Ann Peters, Karan Verma, Jeffrey S. Huo, C. Conover Talbot Jr., Kejin Hu, Igor Slukvin, Renee Reijo-Pera, and Elias T. Zambidis. June 12-15, 2013, Reprogramming of stromal-primed human myeloid progenitors generates hipsc with high fidelity states of pluripotency and augmented multilineage differentiation capacities, ISSCR annual 11th meeting. Boston, Massachusetts, USA.

4. Kejin Hu, Junying Y, James Thomson, Igor Slukvin, Efficient reprogramming of mononuclear cells from archived human bone marrow and cord blood with non-integrating episomal constructs. ISSCR 8th annual meeting, San Francisco, CA, June 16-19, 2010. 5. Kejin Hu and Yu Jiang (July, 2006) Regulation of TOR signaling by ubiquitin. 2006 Yeast Genetics and Molecular Biology Meeting. Princeton University, Princeton, New Jersey, July 25-30, 2006. 6. Nirav Amin, Kejin Hu, David Pruyne, Dino Terzic, Anthony Bretscher, Jun Liu. (2006)

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Fozi-1 encodes a novel protein required for proper cell fate specification in the post-embryonic mesoderm. Development and Evolution Meeting. 7. Nirav M. Amin, Kejin Hu, Dino Terzic, Jun Liu. (2005). Fozi-1, a gene required for proper development of the postembryonic mesoderm. International Worm Meeting. 8. Kejin Hu and Yu Jiang (May, 2005) FRB domain is required for TOR function. A poster at Pharmacology 2005 retreat for Pharmacology Department, the University of Pittsburgh, Seven Spring, Pittsburgh, PA, USA. 9. Kejin Hu, Abby Mak, Michelle Lok-sum Wong and Siu-Ming Chan. (1999) Characterization of the major egg yolk protein of the shrimp Metapenaeus ensis. (Poster: POS-H30). 8th International Symposium of Society of Chinese Bioscientists in America (SCBA). Hong Kong Convention and Exhibition Center, Hong Kong, China. 14-19 August, 1999. 10. K.W. Yeung, K. P. Ho, J.L Hu and Kejin Hu (1997) An evaluation of chitosan extraction from fungi. (Poster: P31). Presented at the Fifth International Conference on the Biotechnology of Microbial Products (BMP’97), held in Williamsburg, VA, USA, April 27-30, 1997. (I am the presenter). 11. K. P. Ho, K.W., Yeung, J. L. Hu and Kejin Hu (1996) Screening of Fungi for Chitosan Producers. Presented at the Second International Symposium and Workshop of Asia Pacific Society of Bioscientists, held at the Hong Kong University of Science and Technology, Hong Kong, China, 8-11 July 1996. (I am the presenter). 12. Kejin Hu* (1995) Analysis of the multi-variables for rice cultivation. Presented at the Fifth Conference of Agricultural System Engineering Association of China.

GenBank publications 1. Kejin Hu and Pak-chow Leung (2004) Access number: AY126712: 12-FEB-2004; Metapenaeus ensis cathepsin L precursor (CatL) mRNA, complete cds. 2. Kejin Hu and Pak-chow Leung (2004) Access number: AY126713; 12-FEB-2004; Metapenaeus ensis cathepsin L precursor (CatL) gene, partial cds. 3. Shao, et al. and Kejin HU*, (2016) The BRD3R cDNA sequence in GenBank under accession code KR633047.

Database deposit Kejin Hu and Zhicheng Shao. Gene expression Omnibus (GEO) repository. RNA-seq, Acessoin #, GSE66798 and GSE75364.

Oral presentations

Invited talks

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1. November 14th, 2018 (Scheduled). The challenge of cellular reprogramming. Webinar organized by the magazine of ‘SCIENTIST”. 2. November 9th, 2018 (Scheduled). The roadblock of iPSC reprogramming. 2nd Annual Summit on Cell Therapy, Tissue Science and Regenerative Medicine”. Atlanta, USA. 3. October 3rd , 2018 (scheduled), Molecular mechanisms of cellular reprogramming. University of Illinois, College of Medicine-Rockford. 4. March 14th, 2018. Stem Cell Therapy. Osher Lifelong Learning Institute (Greater Birmingham area). 5. October 25th, 2017, Man-made stem cells and their promise for cure of diseases. UAB Genetics Club. 6. October 13th, 2016, Symposium for 10 years of iPSC Technology, Beijing, China, invited speaker, How to erase somatic transcriptional program during iPSC reprogramming? 7. July 5th, 2013, Hong Kong University, Hong Kong, China, Invited talk, Identification of roadblocks to human pluripotency. 8. July 9th, Sun Yat-sen University, Guangzhou, China, Invited talk, Identification of roadblocks to human pluripotency. 9. July 21st, Central China Agriculture University, Wuhan, China, Invited talk, Identification of roadblocks to human pluripotency 10. Invited talk. Efficient reprogramming of mononuclear cells from archived human bone marrow and cord blood with non-integrating episomal constructs, WiCell Research Institute, Madison, Wisconsin, March 2nd, 2010

Other talks 1. Reverse genetics for Identification of roadblocks to human pluripotency. Feb. 11th, 2013. BMG seminar series. Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama. 2. Reprogramming normal and neoplastic bone marrow mononuclear cells to pluripotency. Nov. 10th, 2010, Inter-laboratory Group Meetings, UW Molecular Hematology. 3. Efficient generation of induced pluripotent stem cells from normal and neoplastic mononuclear cells of frozen human bone marrow and cord blood samples using non-integrating episomal constructs. September 28th, 2010. Campus Stem Cell Laboratory Meetings, University of Wisconsin Stem Cell & Regenerative Medicine Center. 4. (April 1st, 2004). Rapamycin sensitive signaling in yeast. Pittsburgh Yeast Club, at Carnegie Mellon University, Pittsburgh, PA, USA. (This is a joint meeting of scientists in yeast from Pittsburgh University of Carnegie Mellow University). 5. (2001) Cloning and characterization of cathepsin-L genes of the marine shrimp Metapenaeus ensis. Abstract in p81-82, Abstracts for the Fifth International Crustacean Congress and ‘Summer’ 2001 meeting of the Crustacean Society, 9-13 July, 2001, The University of Melbourne, Melbourne Victoria, Australia.

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Awards 1. Analysis of the epidemic factors of the rice blast in E-xi autonomous prefecture of Hubei. Bronze medal of excellent research papers for undergraduate student in the year of 1985, awarded by Hubei Education Committee. 2. The mathematical modeling and analysis of late rice cultivation in Hubei agro-ecosystem by Kejin Hu Excellent paper awarded in 1995 by the Committee of the Fifth Conference of Agricultural System Engineering Association of China. (This award was granted to me in the absence of me (at the time of conference, I have left mainland China for my master degree in Hong Kong.) 3. Excellent presentation in the First Scientific Conference for Young Scientists of The Institute of Agriculture Modernization, Hubei Academy of Agriculture Sciences. 1989. 4. Third prize of scientific improvement awarded by Hubei Province for the research project of “comprehensive technology for the high and sustainable output from cold-spring submerged rice field”. 1998. 5. Second Prize of Scientific Improvement in Hubei Province for the research project “investigation into optimized model of the agro-ecosystem in Hubei region”. 1995. 6. Scholarship for MPhil program from The Hong Kong Polytechnic University. 1995-1997. 7. Scholarship for PhD program from Hong Kong University. 1999-2003.

Other publications

Kejin Hu (1987) The techniques of canned food production: A textbook for a training course for technicians in the production of canned food (an internal publication by Hubei Academy of Agricultural Sciences)

THESES

1. PhD dissertation title: Cloning and characterization of cathepsin L gene from the marine shrimp Metapenaeus ensis. (Written in English) 2. MPhil dissertation: Production and characterization of fungal chitosan. (Written in English) 3. Dissertation for Bachelor degree: Analysis of the epidemic factors of the rice blast in E-xi autonomous prefecture of Hubei province. (In Chinese).

Grant support

1) UAB provost faculty development program; April 2016-March 2017.

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Title, Changing cell fates via specific transcriptional de-bookmarking

Role, PI Total $10,000.00.

2) Alabama Institute of Medicine, seed grant in stem cell biology, (HU, PI)

06/2014/05/30/2015

Title, Role of PODXL in human pluripotent reprogramming and pluripotent cell biology

Role, PI Total $100,000 (direct $80,000; indirect, $20,000)

3) American Heart Association. AWRP Winter 2017 Grant-in-Aid,

07/01/2017 -06/30/2019. Grant ID#, 17GRNT33670780. (Hu, PI)

Title, Transcriptional resistance to cardiac reprogramming and transcriptional regulation

of cardiac fibroblasts.

Role, PI Total, $154,000.00 ($77,000.00 per year for two years.

$140,000.00 are direct cost, and $14,000.00 are indirect cost).

4) NIH, R01, 1R01GM127411-01A1, 08/01/2018-07/30/2023 .

Role, PI

Title: BET regulation of mitosis and pluripotency establishment.

Direct cost: $210,000 per year for five years.

Postdocs and visiting scholars trained:

Zhicheng Shao (PhD), currently associate professor at Xiamen University. Bo chen (PhD), Currently Principle investigator, Institute of Blood, Chinese Academy of Medical Sciences. Changlong Liu (PhD), Currently Postdoc, University of South Carolina Lei Kang (PhD), Currently postdoc, Baylor College of Medicine, Chunping Yao (MD), currently medical doctor, Shandong Cancer Hospital and Institute. Weihua Xu (PhD), assistant professor, South China Agriculture University.

Current Postdocs

Richardo Cevallos Md Emon Hossain

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