Identification of Novel FNIN2 and FNIN3 Fibronectin-Derived
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
And MMP-Mediated Cell–Matrix Interactions in the Tumor Microenvironment
International Journal of Molecular Sciences Review Hold on or Cut? Integrin- and MMP-Mediated Cell–Matrix Interactions in the Tumor Microenvironment Stephan Niland and Johannes A. Eble * Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, 48149 Münster, Germany; [email protected] * Correspondence: [email protected] Abstract: The tumor microenvironment (TME) has become the focus of interest in cancer research and treatment. It includes the extracellular matrix (ECM) and ECM-modifying enzymes that are secreted by cancer and neighboring cells. The ECM serves both to anchor the tumor cells embedded in it and as a means of communication between the various cellular and non-cellular components of the TME. The cells of the TME modify their surrounding cancer-characteristic ECM. This in turn provides feedback to them via cellular receptors, thereby regulating, together with cytokines and exosomes, differentiation processes as well as tumor progression and spread. Matrix remodeling is accomplished by altering the repertoire of ECM components and by biophysical changes in stiffness and tension caused by ECM-crosslinking and ECM-degrading enzymes, in particular matrix metalloproteinases (MMPs). These can degrade ECM barriers or, by partial proteolysis, release soluble ECM fragments called matrikines, which influence cells inside and outside the TME. This review examines the changes in the ECM of the TME and the interaction between cells and the ECM, with a particular focus on MMPs. Keywords: tumor microenvironment; extracellular matrix; integrins; matrix metalloproteinases; matrikines Citation: Niland, S.; Eble, J.A. Hold on or Cut? Integrin- and MMP-Mediated Cell–Matrix 1. Introduction Interactions in the Tumor Microenvironment. -
Cancer Cell Angiogenic Capability Is Regulated by 3D Culture and Integrin Engagement
Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement Claudia Fischbacha,b, Hyun Joon Konga,c, Susan X. Hsionga, Marta B. Evangelistaa,d,e, Will Yuena, and David J. Mooneya,f,1 aSchool of Engineering and Applied Sciences, Harvard University, 40 Oxford Street, Cambridge, MA 02138; bDepartment of Biomedical Engineering, Cornell University, 157 Weill Hall, Ithaca, NY 14853; cDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana–Champaign, Urbana, IL 61801; dInstituto de Engenharia Biomedica, Divisao de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; eUniversidade do Porto, Faculdade de Engenharia, Departamento de Engenharia Metalu´rgica e de Materiais, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal; and fWyss Institute of Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138 Edited by Robert Langer, Massachusetts Institute of Technology, Cambridge, MA, and approved November 26, 2008 (received for review September 9, 2008) Three-dimensional culture alters cancer cell signaling; however, the regulate VEGF and IL-8 secretion and that these differences underlying mechanisms and importance of these changes on tumor impact tumor vascularization in vivo. These findings serve to vascularization remain unclear. A hydrogel system was used to examine identify 3D integrin engagement as a mechanism that alters cancer the role of the transition from 2D to 3D culture, with and without cell angiogenic signaling and that may be explored toward more integrin engagement, on cancer cell angiogenic capability. Three- efficacious antiangiogenic therapies. dimensional culture recreated tumor microenvironmental cues and led to enhanced interleukin 8 (IL-8) secretion that depended on integrin Results and Discussion engagement with adhesion peptides coupled to the polymer. -
Fibromodulin: Structure, Physiological Functions, and an Emphasis on Its Potential Clinical Applications in Various Diseases Mohammad M
SYSTEMATIC REVIEW ARTICLE Fibromodulin: Structure, Physiological Functions, and an Emphasis on its Potential Clinical Applications in Various Diseases Mohammad M. Al-Qattan and Ahmed M. Al-Qattan ABSTRACT Fibromodulin (FMOD) is one of the small leucine-rich proteoglycans. A search of the literature did not reveal any paper that specifically reviews the potential clinical applications of FMOD in the management of human diseases. First, the structure and physiological functions of FMOD were reviewed. Then its potential clinical applications in various diseases including diseases of the skin, tendons, joints, intervertebral discs, blood vessels, teeth, uterus, bone and kidney were reviewed. FMOD is able to switch the adult response to skin wounding to the desired fetal response of scarless healing. Lowered levels of FMOD would be desirable in the management of tendinopathy, uterine fibroids, tumors resistant to radiotherapy, glioblastomas, small-cell lung cancer, and primary liver/lung fibrosis. In contrast, increased levels of FMOD would be desirable in the management of acute tendon injuries, osteoarthritis, rheumatoid arthritis, temporo-mandibular disease, joint laxity, intervertebral disc disease, neo-intimal hyperplasia of vein grafts, teeth caries, periodontal disease, endometrial atrophy, osteoporosis and diabetic nephropathy. Furthermore, FMOD may be used as a prognostic marker of cerebrovascular events in patients undergoing carotid endarterectomy and a marker for prostatic cancer. Finally, the use of FMOD in the treatment of symptomatic endometrial atrophy should be explored in women who are unable to use the standard estrogen management for endometrial atrophy. The review concluded that clinical trials in humans should be initiated to investigate the potential therapeutic effects of FMOD. -
Versao Definitiva Jun2017
UNIVERSIDADE DE LISBOA FACULDADE DE FARMÁCIA Development of three-dimensional umbilical cord-derived mesenchymal stem cell cultures for differentiation into hepatocyte-like cells: a potential breakthrough in toxicological drug screening Madalena Zincke dos Reis Fernandes Cipriano Orientadora: Doutora Joana Paiva Gomes Miranda Co-orientadores: Doutor Jorge Miguel Silva Santos Doutora Katrin Zeilinger Tese especialmente elaborada para a obtenção do grau de Doutor em Farmácia, especialidade de Toxicologia 2017 UNIVERSIDADE DE LISBOA FACULDADE DE FARMÁCIA Development of three-dimensional umbilical cord-derived mesenchymal stem cell cultures for differentiation into hepatocyte-like cells: a potential breakthrough in toxicological drug screening Madalena Zincke dos Reis Fernandes Cipriano Orientadora: Doutora Joana Paiva Gomes Miranda Co-orientadores: Doutor Jorge Miguel Silva Santos Doutora Katrin Zeilinger Tese especialmente elaborada para a obtenção do grau de Doutor em Farmácia, especialidade de Toxicologia Júri: Presidente: Doutora Matilde da Luz dos Santos Duque da Fonseca e Castro, Professora Catedrática e Directora da Faculdade de Farmácia da Universidade de Lisboa Vogais: Doutor Jorge Manuel Lira Gonçalves Ruas, Associate Professor, Karolinska Institutet, Sweden Doutor Félix Dias Carvalho, Professor Catedrático, Faculdade de Farmácia da Universidade do Porto Doutora Diana Esperança dos Santos Nascimento, Investigadora Auxiliar, Instituto de Engenharia Biomédica da Universidade do Porto Doutora Sofia de Azeredo Pereira Costa, Professora -
A Decade of Organs-On-A-Chip Emulating Human Physiology at the Microscale: a Critical Status Report on Progress in Toxicology and Pharmacology
micromachines Review A Decade of Organs-on-a-Chip Emulating Human Physiology at the Microscale: A Critical Status Report on Progress in Toxicology and Pharmacology Mario Rothbauer 1,2,3,*, Barbara E.M. Bachmann 1,2,4 , Christoph Eilenberger 1,2, Sebastian R.A. Kratz 1,2,5, Sarah Spitz 1,2, Gregor Höll 1,2 and Peter Ertl 1,2,* 1 Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry and Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9/163-164, 1060 Vienna, Austria; [email protected] (B.E.M.B.); [email protected] (C.E.); [email protected] (S.R.A.K.); [email protected] (S.S.); [email protected] (G.H.) 2 Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria 3 Karl Chiari Lab for Orthopaedic Biology, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Währinger Gürtel 18-22, 1090 Vienna, Austria 4 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Allgemeine Unfallversicherungsanstalt (AUVA) Research Centre, Donaueschingenstraße 13, 1200 Vienna, Austria 5 Drug Delivery and 3R-Models Group, Buchmann Institute for Molecular Life Sciences & Institute for Pharmaceutical Technology, Goethe University Frankfurt Am Main, 60438 Frankfurt, Germany * Correspondence: [email protected] (M.R.); [email protected] (P.E.) Citation: Rothbauer, M.; Abstract: Organ-on-a-chip technology has the potential to accelerate pharmaceutical drug devel- Bachmann, B.E.M.; Eilenberger, C.; opment, improve the clinical translation of basic research, and provide personalized intervention Kratz, S.R.A.; Spitz, S.; Höll, G.; Ertl, P. -
3D Cardiac Cell Culture: a Critical Review of Current Technologies and Applications
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Bern Open Repository and Information System (BORIS) REVIEW published: 26 June 2019 doi: 10.3389/fcvm.2019.00087 3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications Christian Zuppinger* Cardiology, Department of Biomedical Research, Bern University Hospital, Bern, Switzerland Three-dimensional (3D) cell culture is often mentioned in the context of regenerative medicine, for example, for the replacement of ischemic myocardium with tissue-engineered muscle constructs. Additionally, 3D cell culture is used, although less commonly, in basic research, toxicology, and drug development. These applications have recently benefited from innovations in stem cell technologies allowing the mass-production of hiPSC-derived cardiomyocytes or other cardiovascular cells, and from new culturing methods including organ-on-chip and bioprinting technologies. On the analysis side, improved sensors, computer-assisted image analysis, and data collection techniques have lowered the bar for switching to 3D cell culture models. Nevertheless, 3D cell culture is not as widespread or standardized as traditional cell culture methods using monolayers of cells on flat surfaces. The many possibilities Edited by: of 3D cell culture, but also its limitations, drawbacks and methodological pitfalls, are Maurizio Pesce, Centro Cardiologico Monzino less well-known. This article reviews currently used cardiovascular 3D cell culture (IRCCS), Italy production -
Cell Culture in Three-Dimensional Lattices of Hydrated Collagen Fibrils − Its Progress and Perspectives
N. Saito et al., Int. J. of Design & Nature and Ecodynamics. Vol. 10, No. 1 (2015) 1–9 CELL CULTURE IN THREE-DIMENSIONAL LATTICES OF HYDRATED COLLAGEN FIBRILS − ITS PROGRESS AND PERSPECTIVES N. SAITO1, H. ADACHI2, H. TANAKA2, S. NAKATA2, N. KAWADA1 & K. YOSHIZATO1,3 1Department of Hepatology, Osaka City University, Japan. 2Nippon Menard Cosmetic Co. Ltd., Japan. 3PhoenixBio Co. Ltd., Japan ABSTRACT Cell culture methodology has been utilized as a powerful tool to characterize cells with a presupposition that the cells in culture faithfully reproduce their in vivo phenotypes. However, it is generally recognized that the cells cultured currently by the most commonly utilized two-dimensional (2D) monolayer technology exhibit different phenotypes from those in living tissues. Cultivation of cells in three-dimensional (3D) lattices of collagen fibrils (collagen gel culture) has been thought to overcome the shortages of 2D cell culture such that the cells behave as in vivo by interacting with not only nearby other cells but also by surrounding extracellular matrices. A remarkable outcome of studies on collagen gel culture was a demonstration of contractile nature of the cells. One of the not-fully appreciated issues about the 3D cell culture is the effect of fluid flow through the collagen gels on cells’ phenotypes. In this review, we make a short overview of historical and current studies of the collagen gel culture from a viewpoint of ‘how we can more correctly extract the cell-related phenomena in living tissues/organs by in vitro culture technology, placing an emphasis on the importance of stimuli caused by flow of cell culture medium.’ Keywords: Collagen gel culture, connective tissue modeling, ECM compositions, gel contraction, interstitial fluid flow, tensile strength. -
3D Cell Culture-Based Global Mirna Expression Analysis Reveals Mir-142-5P As a Theranostic Biomarker of Rectal Cancer Following Neoadjuvant Long-Course Treatment
biomolecules Article 3D Cell Culture-Based Global miRNA Expression Analysis Reveals miR-142-5p as a Theranostic Biomarker of Rectal Cancer Following Neoadjuvant Long-Course Treatment 1,2, 1,3, , 1,4,5 1 Linas Kunigenas y, Vaidotas Stankevicius * y, Audrius Dulskas , Elzbieta Budginaite , Gediminas Alzbutas 6,7, Eugenijus Stratilatovas 1,4, Nils Cordes 8,9,10,11,12 and Kestutis Suziedelis 1,2,* 1 National Cancer Institute, LT-08660 Vilnius, Lithuania; [email protected] (L.K.); [email protected] (A.D.); [email protected] (E.B.); [email protected] (E.S.) 2 Life Sciences Center, Institute of Biosciences, Vilnius University, LT-08412 Vilnius, Lithuania 3 Life Sciences Center, Institute of Biotechnology, Vilnius University, LT-08412 Vilnius, Lithuania 4 Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, LT-08406 Vilnius, Lithuania 5 University of Applied Sciences, Faculty of Health Care, LT-08303 Vilnius, Lithuania 6 Thermo Fisher Scientific, LT-02241 Vilnius, Lithuania; gediminas.alzbutas@thermofisher.com 7 Institute of Informatics, Faculty of Mathematics and Informatics, Vilnius University,LT-08303 Vilnius, Lithuania 8 OncoRay—National Center for Radiation Research in Oncology, Faculty of Medicine, Technische Universität, D–01307 Dresden, Germany; [email protected] 9 Department of Radiation Oncology, University Hospital Carl Gustav Carus, Technische Universität, D–01307 Dresden, Germany 10 Helmholtz–Zentrum Dresden–Rossendorf, Institute of Radiooncology–OncoRay,D–01328 Dresden, Germany 11 German Cancer Consortium (DKTK), partner site Dresden, D–69192 Heidelberg, Germany 12 German Cancer Research Center (DKFZ), D–69192 Heidelberg, Germany * Correspondence: [email protected] (V.S.); [email protected] (K.S.); Tel.: +370-5219-0904 (K.S.) These authors contributed equally to the work. -
3D Neural Tissue Models : from Spheroids to Bioprinting
This document is downloaded from DR‑NTU (https://dr.ntu.edu.sg) Nanyang Technological University, Singapore. 3D neural tissue models : from spheroids to bioprinting Zhuang, Pei; Sun, Alfred Xuyang; An, Jia; Chua, Chee Kai; Chew, Sing Yian 2018 Zhuang, P., Sun, A. X., An, J., Chua, C. K., & Chew, S. Y. (2018). 3D neural tissue models : from spheroids to bioprinting. Biomaterials, 154113‑133. doi:10.1016/j.biomaterials.2017.10.002 https://hdl.handle.net/10356/86209 https://doi.org/10.1016/j.biomaterials.2017.10.002 © 2018 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Biomaterials, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.biomaterials.2017.10.002]. Downloaded on 30 Sep 2021 00:10:53 SGT Review 3D Neural Tissue Models: From Spheroids to Bioprinting Pei Zhuang1#, Alfred Xuyang Sun2,3,#, Jia An1, Chee Kai Chua1,* and Sing Yian Chew4,5,* 1 Singapore Centre for 3D Printing, School of Mechanical and Aerospace Engineering, Nanyang Technological University; [email protected] ; [email protected] ; [email protected] 2 Department of Neurology, National Neuroscience Institute, 20 College Road, Singapore 169856; [email protected] 3 Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672 4 School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore ; [email protected] 5Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232, Singapore # The author contributes the same * Correspondence: [email protected] ; [email protected] Tel.: +65-6316 8812 Abstract: Three-dimensional (3D) in vitro neural tissue models provide a better recapitulation of in vivo cell- cell and cell-extracellular matrix interactions than conventional two-dimensional (2D) cultures. -
Recombinant Fibromodulin and Decorin Effects on NF-Κb and Tgfβ1 in the 4T1 Breast Cancer Cell Line
ONCOLOGY LETTERS 13: 4475-4480, 2017 Recombinant fibromodulin and decorin effects on NF-κB and TGFβ1 in the 4T1 breast cancer cell line LADAN DAWOODY NEJAD1,2, ALIREZA BIGLARI3, TIZIANA ANNESE4 and DOMENICO RIBATTI4,5 1Department of Molecular Medicine and Biochemistry Institute, University of Bern, 3012 Bern, Switzerland; Departments of 2Molecular Medicine and Genetics and 3Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, 45154 Zanjan, Iran; 4Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Section of Human Anatomy and Histology, University of Bari Medical School, I-70124 Bari; 5National Cancer Institute Giovanni Paolo II, I-70126 Bari, Italy Received October 18, 2016; Accepted February 3, 2017 DOI: 10.3892/ol.2017.5960 Abstract. Constitutive activation of nuclear factor-κB NF-κB activity stimulating signals cause dissociation of IκB, (NF-κB) stimulates cell proliferation and metastasis, and allowing NF-κB dimers to locate to the nucleus and alter inhibits apoptosis in breast cancer. Transforming growth gene expression (6). Additionally, NF-κB signaling is essen- factor-β (TGF-β) signaling pathway is deregulated in breast tial for epithelial-mesenchymal transition (EMT), and the cancer progression and metastasis. The aim of the present therapeutic inhibition of NF-κB may be an effective strategy study was to investigate the inhibitory effects of the two small to control tumor invasion and metastasis (7). leucine rich proteoglycans fibromodulin (Fmod) and decorin Transforming growth factor β (TGF-β) is a pleiotropic (Dcn), overexpressed using adenovirus gene transfer, on cytokine that is found in three isoforms (TGF-β1, TGF-β2 NF-κB-activity and TGF-β1-expression in the highly meta- and TGF-β3), which are structurally and functionally static 4T1 breast cancer cell line. -
Collagen-Binding Proteoglycan Fibromodulin Can Determine Stroma Matrix Structure and Fluid Balance in Experimental Carcinoma
Collagen-binding proteoglycan fibromodulin can determine stroma matrix structure and fluid balance in experimental carcinoma Åke Oldberg*, Sebastian Kalamajski*, Alexei V. Salnikov†‡§, Linda Stuhr¶, Matthias Mo¨ rgelinʈ, Rolf K. Reed¶, Nils-Erik Heldin**, and Kristofer Rubin†,†† *Department of Experimental Medical Sciences, BMC, B-12, and ʈDepartment of Clinical Sciences, BMC B14, University of Lund, SE-221 84 Lund, Sweden; †Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, SE-751 23 Uppsala, Sweden; ‡Oncology Clinic, University Hospital Lund, SE-221 85 Lund, Sweden; ¶Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway and **Department of Genetics and Pathology, Uppsala University Hospital, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden Edited by Erkki Ruoslahti, Burnham Institute for Medical Research, Santa Barbara, CA, and approved July 16, 2007 (received for review March 7, 2007) Research on the biology of the tumor stroma has the potential to Clara, CA) of Fc:TRII-treated KAT-4 carcinomas revealed lead to development of more effective treatment regimes enhanc- down-regulations predominantly of macrophage-related genes ing the efficacy of drug-based treatment of solid malignancies. (17). Furthermore, inhibition of IL-1 reduces IFP in these Tumor stroma is characterized by distorted blood vessels and carcinomas (17). These findings indicate a role of inflammation activated connective tissue cells producing a collagen-rich matrix, in the generation of an elevated IFP. Tortuous and leaky blood which is accompanied by elevated interstitial fluid pressure (IFP), vessels, and absence of lymph drainage in tumors have also been indicating a transport barrier between tumor tissue and blood. -
In Vitro 3D Angiogenesis Assay in Egg White Matrix
Laboratory Investigation (2014) 94, 340–349 & 2014 USCAP, Inc All rights reserved 0023-6837/14 In vitro 3D angiogenesis assay in egg white matrix: comparison to Matrigel, compatibility to various species, and suitability for drug testing Yoanne Mousseau1,Se´verine Mollard1, Hao Qiu1, Laurence Richard1, Raphael Cazal1, Ange´lique Nizou1, Nicolas Vedrenne1,Se´verine Re´mi2, Yasser Baaj1, Laurent Fourcade1, Benoit Funalot1 and Franck G Sturtz1 In vitro angiogenesis assays are commonly used to assess pro- or anti-angiogenic drug properties. Extracellular matrix (ECM) substitutes such as Matrigel and collagen gel became very popular in in vitro 3D angiogenesis assays as they enable tubule formation by endothelial cells from culture or aortic rings. However, these assays are usually used with a single cell type, lacking the complex cellular interactions occurring during angiogenesis. Here, we report a novel angiogenesis assay using egg white as ECM substitute. We found that, similar to Matrigel, egg white elicited prevascular network formation by endothelial and/or smooth muscle cell coculture. This matrix was suitable for various cells from human, mouse, and rat origin. It is compatible with aortic ring assay and also enables vascular and tumor cell coculture. Through simple labeling (DAPI, Hoechst 33258), cell location and resulting prevascular network formation can easily be quantified. Cell trans- fection with green fluorescent protein improved whole cell visualization and 3D structure characterization. Finally, egg- based assay dedicated