SAC Phosphoinositide Phosphatases at the Tonoplast Mediate Vacuolar Function in Arabidopsis
SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis Petra Novákováa,b,c, Sibylle Hirschb,c,1, Elena Ferarub,c,2, Ricardo Tejosb,c,3, Ringo van Wijkd, Tom Viaeneb,c, Mareike Heilmanne, Jennifer Lerchee, Riet De Ryckeb,c, Mugurel I. Ferarub,c,2, Peter Gronesa,b,c, Marc Van Montagub,c,4, Ingo Heilmanne, Teun Munnikd, and Jirí Frimla,b,c,4 aInstitute of Science and Technology Austria, 3400 Klosterneuburg, Austria; bDepartment of Plant Systems Biology, Flanders Institute for Biotechnology and cDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; dSection of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1090 GE, Amsterdam, The Netherlands; and eDepartment of Cellular Biochemistry, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, 06099 Halle, Germany Contributed by Marc Van Montagu, January 2, 2014 (sent for review December 4, 2013) Phosphatidylinositol (PtdIns) is a structural phospholipid that can function of this minor phospholipid. Recent observations also be phosphorylated into various lipid signaling molecules, desig- hint at a role for PPIs in plant vacuoles (18–20), but the data are nated polyphosphoinositides (PPIs). The reversible phosphoryla- scarce and remain inconclusive. tion of PPIs on the 3, 4, or 5 position of inositol is performed by Advances in deciphering various cellular roles of PIs include a set of organelle-specific kinases and phosphatases, and the insights into the phosphatases responsible for hydrolyzing PPIs. characteristic head groups make these molecules ideal for regu- A group of phosphatases, designated suppressor of actin (SAC) lating biological processes in time and space.
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