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Genetic Characterization of Angiostrongylus

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Genetic Characterization of Angiostrongylus Genetic Characterization of Angiostrongylus Cantonensis Isolates from Different Regions of Ecuador Introduction The genetic aspects of this parasite Detection and Identification. En Methods Invasive Snails and an Emerging Instituto Oswaldo Cruz, 90(5), 605-609. Thiengo, S. C., de Oliveira Simões, R., Fernandez, Caracterización Genética de Angiostrongylus Cantonensis have been explored in a systematic and in Microbiology (Vol. 42, pp. 525-554). Infectious Disease: Results from the First https://doi.org/10.1590/S0074-02761995 M. A., & Júnior, A. M. (2013). phylogenic way. The sequences of Elsevier. https://doi.org/10.1016/bs.mim. National Survey on Angiostrongylus 000500011 Angiostrongylus cantonensis and Rat Angiostrongylus cantonensis was first 2015.06.004 cantonensis in China. PLOS Neglected Lungworm Disease in Brazil. Hawai’i Aislados de Diferentes Regiones de Ecuador described in rats in Guangzhou (Canton), nuclear and mitochondrial genes have Tropical Diseases, 3(2), e368. Pincay, T., García, L., Narváez, E., Decker, O., Journal of Medicine & Public Health, Luis Solórzano Alava 1, Cesar Bedoya Pilozo 2, Hilda Hernández Alvarez 3, Misladys Rodriguez 4, Lazara Rojas Rivero5, Francisco Sánchez China, in 1935 (Chen, 1935). This been used for molecular differentiation Galtier, N., Nabholz, B., Glémin, S., & Hurst, G. https://doi.org/10.1371/journal.pntd.0000 Martini, L., & Moreira, J. (2009). 72(6 Suppl 2), 18-22. Amador 6, Marcelo Muñoz Naranjo 7, Cecibel Ramirez 8, Rita Loja Chango 9, José Pizarro Velastegui 10, Alessandra Loureiro Morasutti 11 nematode also infects humans and is the and phylogenetic analyzes of D. D. (2009). Mitochondrial DNA as a 368 Angiostrongiliasis por Parastrongylus INFORMACIÓN DEL Abstract main cause of eosinophilic Angiostrongylus species (Galtier et al., marker of molecular diversity: A (Angiostrongylus) cantonensis en Tokiwa, T., Harunari, T., Tanikawa, T., Komatsu, ARTÍCULO reappraisal. Molecular Ecology, 18(22), Martini-Robles L, Gomez E, Muzzio J, Solorzano Ecuador. Primer informe en Sudamérica. N., Koizumi, N., Tung, K.-C., Suzuki, J., Angiostrongylus cantonensis (Chen, 1945) was identified in Ecuador for meningoencephalitis, an infectious 2009) Fecha de recepción: noviembre 5, 2020. the first time in 2008. Eleven provinces of the country were investigated to 4541-4550. https://doi.org/10.1111/j.136 L. (2016). Descripcion del primer foco de Tropical Medical Int Health, 14(2), S37. Kadosaka, T., Takada, N., Kumagai, T., Fecha de aceptación: febrero1, 2021. disease of the central nervous system established the phylogenetic relationships between the isolates of the adult 5-294X.2009.04380.x transmision natural de Angiostrongylus Akao, N., & Ohta, N. (2012). 1 Especialista en laboratorio Clinico, Instituto (CNS) and its membranes, characterized, The mitochondrial COI gene cantonensis en Ecuador. En A. Martini - Rodpai, R., Intapan, P. M., Thanchomnang, T., Phylogenetic relationships of rat Nacional de Investigación en Salud Pública parasite and to compare them with those existing in other regions of the “INSPI”, Centro de Referencia Nacional de among other aspects, by an increase in the represents a good marker for the study of Kearse, M., Moir, R., Wilson, A., Stones-Havas, Robles, Dorta - Contreras, Angiostrongylus Sanpool, O., Sadaow, L., Laymanivong, lungworm, Angiostrongylus cantonensis, Parasitología y Micología y Dirección de Investigación, world. Partial region of subunit 1 of Cytochrome oxidase (COI 1) was Docencia e Innovación, Guayaquil, Ecuador S., Cheung, M., Sturrock, S., Buxton, S., cantonensis. Emergencia en América (pp. S., Aung, W. P., Phosuk, I., isolated from different geographical E-mail: [email protected] amplified, amplicons were sequenced and aligned with the reference proportion of eosinophils in the genetic evolution (phylogeny) of the Código ORCID: Cooper, A., Markowitz, S., Duran, C., 209-220). Laummaunwai, P., & Maleewong, W. regions revealed widespread multiple https://orcid.org/0000-0002-9758-6663 different strains of A. cantonensis, and in sequences of the AC1-AC9 haplotypes. Isolates are grouped in a single cerebrospinal fluid and later in peripheral Thierer, T., Ashton, B., Meintjes, P., & (2016). Angiostrongylus cantonensis and lineages. Parasitology International, 2 the identification of haplotypes of the Magister en Biotecnología molecular, clade different from the other previously characterized groups, which blood (Lindo et al., 2004). Since the first Drummond, A. (2012). Geneious Basic: Monte, T. C., Simões, R. O., Oliveira, A. P. M., A. malaysiensis Broadly Overlap in 61(3), 431-436. https://doi.org/10.1016 Universidad de Guayaquil, Hospital “Luis Vernaza”, Servicio de Laboratorio clínico y represents a novelty for the genetic diversity of the parasite in Latin notification of human infection by A. parasite (Monte et al., 2012). An integrated and extendable desktop Novaes, C. F., Thiengo, S. C., Silva, A. J., Thailand, Lao PDR, Cambodia and /j.parint.2012.02.005 Laboratorio de Biología Molecular, Guayaquil, Ecuador. America and the rest of the world. The parasite has been recently cantonensis in Taiwan in 1945, more than software platform for the organization Estrela, P. C., & Júnior, A. M. (2012). Myanmar: A Molecular Survey of Larvae Código ORCID: and analysis of sequence data. Phylogenetic relationship of the Brazilian in Land Snails. PloS One, 11(8), Wallace, G. D., & Rosen, L. (1967). Studies on https://orcid.org/0000-0003-0448-8608 introduced, which has prevented genetic divergence between the isolates. 2,800 cases have been registered in The main objective of the study was Bioinformatics, 28(12), 1647-1649. isolates of the rat lungworm e0161128. https://doi.org/10.1371/jour eosinophilic meningitis. IV. Experimental 3 establish the phylogenetic relationships Ph.D en Ciencias, Instituto de Medicina different regions of the world (Wang et https://doi.org/10.1093/bioinformatics/bt Angiostrongylus cantonensis (Nematoda: nal.pone.0161128 infection of fresh-water and marine fish Tropical Pedro Kouri. Keywords: Código ORCID: al., 2008). of isolates of the parasite from different s199 Metastrongylidae) employing with Angiostrongylus cantonensis. https://orcid.org/0000-0003-0448-8608 Angiostrongylus cantonensis, phylogeny, COI 1, Ecuador. geographic locations in Ecuador, in mitochondrial COI gene sequence data. Simberloff, D., & Rejmanek, M. (Eds.). (2019). American Journal of Epidemiology, 4 JEL Classification: I10, I19. Magister en Epidemiologia, Instituto de Kumar, S., Stecher, G., Li, M., Knyaz, C., & Parasites & Vectors, 5, 248. 100 of the World’s Worst Invasive Alien 85(3), 395-402. Medicina Tropical Pedro Kouri. In 2008, the first focus of natural relation to the isolates characterized in Código ORCID: Tamura, K. (2018). MEGA X: Molecular https://doi.org/10.1186/1756-3305-5-248 Species: A Selection From The Global https://doi.org/10.1093/oxfordjournals.aj https://orcid.org/0000-0001-9455-6103 Resumen transmission of A. cantonensis was South America and the rest of the world. Evolutionary Genetics Analysis across Invasive Species Database. En e.a120701 5 Angiostrongylus cantonensis (Chen, 1945) fue identificado en Ecuador described in Ecuador and the parasite was This study will allow us, firstly, to Ph.D en Ciencias Médicas, Instituto de Computing Platforms. Molecular Biology Moreira, V. L. C., Giese, E. G., Melo, F. T. V., Encyclopedia of Biological Invasions Medicina Tropical Pedro Kouri. Código ORCID: por primera vez en 2008. Se investigaron once provincias del país para found in land snails, Achatina fulica determine if there is genetic variation and Evolution, 35(6), 1547-1549. Simões, R. O., Thiengo, S. C., (pp. 715-716). University of California Wang, Q.-P., Lai, D.-H., Zhu, X.-Q., Chen, X.-G., https://orcid.org/0000-0002-8070-5419 establecer las relaciones filogenéticas entre los aislados del parásito y ((Bowdich, 1922) as well as in rodents among the characterized Ecuadorian https://doi.org/10.1093/molbev/msy096 Maldonado, A., & Santos, J. N. (2013). Press. https://california.degruyter.com/ & Lun, Z.-R. (2008). Human 6 Biólogo, Universidad de Guayaquil. compararlos con los existentes en otras regiones del mundo. Se amplificó Endemic angiostrongyliasis in the view/book/9780520948433/10.1525/978 angiostrongyliasis. The Lancet. Infectious Código ORCID: Rattus rattus (Linnaeus, 1758) and Rattus isolates and secondly, to establish https://orcid.org/0000-0003-3566-6964 la región parcial de la subunidad 1 del citocromo oxidasa (COI 1), se norvegicus (Berkenhout, 1769) (Martini- phylogenetic relationships with variants Legislation for the protection of animals used for Brazilian Amazon: Natural parasitism of 0520948433-159.xml Diseases, 8(10), 621-630. 7 secuenciaron los amplicones y se alinearon con las secuencias de scientific purposes - Environment - Angiostrongylus cantonensis in Rattus https://doi.org/10.1016/S1473-3099(08)7 PhD en Fisiologia de invertebrados, Robles L, Gomez E, Muzzio J, described in different regions of the Universidad de Montpellier. referencia de los haplotipos AC1-AC9. Los resultados demuestran que los European Commission. (s. f.). rattus and R. norvegicus, and sympatric Simões, R. O., Júnior, A. M., Olifiers, N., Garcia, 0229-9 Código ORCID: Solorzano L., 2016) and in human world. This information will allow us to https://orcid.org/0000-0002-5379-5594 aislamientos se agrupan en un solo clado diferente a los otros grupos Recuperado 29 de octubre de 2020, de giant African land snails, Achatina fulica. J. S., Bertolino,
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