(Coloradia Pandora) on the Kaibab National Forest & Armillaria
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MONITORING PANDORA MOTH (COLORADIA PANDORA) ON THE KAIBAB NATIONAL FOREST & ARMILLARIA ROOT DISEASE DISTRIBUTION IN NORTHERN ARIZONA BY: Christian Hoffman A PROFESSIONAL PAPER SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTERS OF FORESTRY NORTHERN ARIZONA UNIVERSITY June 2013 Committee: Richard W. Hofstetter, Ph. D. , Chair Robert L. Mathiasen, Ph. D. Ryan P. Hanavan, Ph. D. a 1 Professional Paper Abstract 2 3 Pandora moth, Coloradia pandora Blake 1863 (Lepidoptera, Saturniidae), is the largest 4 member of the Coloradia genus in the United States and one of the few that is recognized as a 5 pest. Outbreaks of C. pandora occur in 20 to 30 year intervals lasting 6 to 8 years. Large areas of 6 western coniferous pine forests are defoliated during outbreaks, however, defoliation rarely leads 7 to tree mortality due to the moth’s two year life cycle; however, defoliation can reduce the trees 8 defenses allowing them to succumb to other agents. I monitored pandora moth population 9 density, developed new monitoring techniques, and examined natural enemy populations, on the 10 Kaibab National Forest, Arizona. My monitoring results from 2010 to 2012 demonstrated that 11 the pandora moth population was distributed over several thousand hectares but larval densities 12 were low relative to previous outbreak densities in the 1980’s and no significant defoliation had 13 occurred. Because the population density was lower than previously monitored outbreak 14 populations, new techniques were needed to monitor larvae densities and defoliation; rather than 15 using single tree sampling, multiple small trees were examined for larval presence and 16 defoliation levels. Average larval density per tree was 0.5 (± 0.8) with the greatest number of 17 larvae per tree at 4. Larval density per location did not exceed 60 larvae per ~0.4 ha plots. 18 Defoliation was less than 1% with an average of 8 needles defoliated per tree. Mortality of late 19 instar larvae from parasitoid and NPV infection was 1% and 1%, respectively. Parasitization 20 rates of pupae were 2%. Other mortality factors such as climate, vertebrate predation rates, egg 21 and early instar predation and parasitization rates were not monitored. i 1 A second project I conducted examined the distribution and identification of Armillaria 2 root disease in northern Arizona. Armillaria root disease was sampled on two national forests in 3 northern Arizona utilizing USDA Forest Service Forest Inventory and Analysis permanent plots. 4 Samples of Armillaria were identified using DNA sequences and were determined to consist of a 5 single species, Armillaria ostoyae (Romagnes) Herink. Armillaria root disease was more 6 commonly found in mixed conifer and subalpine forests and was rare in ponderosa pine forests, 7 which was consistent with previous studies conducted in the Southwest. ii 1 Acknowledgements 2 I am eternally grateful to Dr. Richard Hofstetter for his guidance, patience, and support 3 throughout my graduate studies and regarding this project. I thank my committee members Dr. 4 Robert Mathiasen and Dr. Ryan Hanavan for their input, assistance and review of my research 5 and professional paper. I thank Mary Lou Fairweather (USFS) and Ned Klopfenstien (USFS) for 6 all their help with the Armillaria project. I express thanks to the US Forest Service Forest Health 7 Monitoring Evaluation Monitoring program and the McIntire-Stennis Mission Research 8 Program, School of Forestry, Northern Arizona University for funding this research, and thank 9 the Rocky Mountain Research Station in Flagstaff for laboratory space. I appreciate that Ron 10 Sieg (Arizona Game and Fish) permitted me to use the Arizona Game and Fish Cabin for 11 summer housing. I also thank Gary Domis (Kaibab National Forest) for his assistance locating 12 plots and trap sites. 13 iii 1 Table of Contents 2 Paper Abstract i 3 Acknowledgments iii 4 List of Tables and Figures vi 5 Preface x 6 Paper Introduction 1 7 Chapter I: A review of pandora moth biology and impacts 8 Introduction 4 9 Life History/ Description/ Behavior 9 10 Defoliation 14 11 Pandora moth on the North Kaibab15 12 References 17 13 List of Tables and Figures 20 14 Chapter II: Monitoring pandora moth (Coloradia pandora davisi, Lepidoptera: 15 Saturniidae) in Arizona: 2010 to 2012 16 Introduction 29 17 Methods 31 18 Results 33 19 Discussion 36 iv 1 Future Work 38 2 References 39 3 List of Tables and Figures 41 4 Chapter III: Armillaria Root Disease in Northern Arizona 5 Introduction 54 6 Methods 57 7 Results 59 8 Discussion 62 9 References 67 10 List of Tables and Figures 70 11 Paper Conclusions 78 12 v 1 List of Tables and Figures 2 Chapter I 3 Figure 1.1. Coloradia species 1. C. doris 2. C. doris 3. C. velda 4. C. pandora 19 4 pandora 5. C. pandora davisi 6. C. pandora pandora 7. C. doris 5 8. C. doris 9. C. velda 10. C. pandora pandora 11. C. pandora davisi 6 12. C. pandora pandora 13. C. luski 14. C. luski 15 C. luski 16. C. 7 luski 17. C. velda 18. C. doris. The red box highlights male and 8 female C. p. pandora and C. p. davisi (males have plumose antennae) 9 (adapted from Tuskes 1996). 10 Figure 1.2. Pandora moth distribution adapted from Tuskes et al. (1996) for 20 11 pandora moth species; Coloradia pandora pandora is shown as light 12 grey and Coloradia pandora davisi is shown as black. 13 Figure 1.3. Pandora moth eggs collected on the North Kaibab RD. Eggs are 2. 5 21 14 mm long by 2. 0 mm in width; flattened on upper 15 and lower surfaces. Pictures taken by C. Hoffman (left) and R. P. 16 Hanavan (right) North Kaibab RD August, 2010. 17 Figure 1.4. Newly emerged 1st instar pandora moth larvae. Picture taken by R. P. 22 18 Hanavan 2010. 19 Figure 1.5. Pandora moth larvae (second instar) feeding gregariously on ponderosa 23 20 pine needles in the laboratory. Picture taken by R. P. Hanavan 2010. 21 Figure 1.6. Pandora moth third, fourth, and fifth instar larvae. Pictures of third & 24 22 fourth instar larvae taken by C. Hoffman 2011; fifth instar image 23 from USDA Forest Service Archive, USDA Forest Service Bugwood. org. vi 1 Figure 1.7a. A) Pandora moth larvae (5th instar) preparing to pupate, B) Final 25 2 molt from 5th instar larvae, and C) fresh pandora moth pupa. Picture 3 by Nick Aflitto 2012. 4 Figure 1.8. Extent of moderate and severe defoliation by pandora moth on the 26 5 Kaibab National Forest from 1979 to 1983. Adapted from Schmid 6 and Bennett (1988). 7 Chapter II 8 Figure 2.1. Pandora moth distribution adapted from Tuskes et al. (1996) for 40 9 pandora moth species. 10 Figure 2.2. Moderate to severe defoliation by pandora moth on the North Kaibab 41 11 RD from 1979 to1983. Adapted from Schmid and Bennett (1988). 12 Figure 2.3. Ponderosa pine range on the Kaibab National Forest. 42 13 Figure 2.4. Pandora moth larvae plot locations sampled in 2011 on the North Kaibab 43 14 RD. 136 plots were sampled. 15 Figure 2.5. Location of light traps set up near Jacob Lake, AZ in 2010, 2011, 44 16 and 2012. 17 Figure 2.6. Pupal parasite trap pair locations on North Kaibab RD. One set of 45 18 200 pupae from 20 locations was collected in May 2011 and the 19 remaining set of 200 pupae was collected in October 2011. 20 Figure 2.7. Pandora moth larvae plots sampled on the North Kaibab RD in 46 21 2011 shown in relation to the maximum extent from 1979 - 1983 22 outbreak. Plot color depicts the quartile abundance of larvae collected. vii 1 Figure 2.8. Prediction surface produced for 2011 North Kaibab RD pandora moth 47 2 larval density distribution using ARCIS 10. 1 geostatistical analyst 3 universal kriging (regression function (0.36 * x + 2.13)) using larval 4 density data points. 5 6 Figure 2. 8b. Frequency distribution of larval plot data relating to the number of 48 7 larvae per plot and number of needles eaten. 8 Figure 2.9a. Average daily captures of adult moths at three light traps for 2010, 49 9 2011, and 2012. North Kaibab RD. A total of 24,254 moths were 10 captured in2010, 156 moths were captured in 2011, and 8,006 moths 11 were captured in 2012. For each year, the first adult was captured on: 12 21 July 2010, 16 August 2011, and 14 July 2012. 13 14 Figure 2.9b. The percentage of females in the 2010 and 2012 pandora moths 50 15 captured in light traps in the North Kaibab RD. Red dash lines 16 are predicted values used to complete curves. 17 18 Figure 2.10. Average daily adult moth captures in light trap for 2012, Tusayan 51 19 Ranger District, Kaibab National Forest. A total of 1,218 moths were 20 captured. The first adult was captured on 11 August 2012. 21 22 Chapter III 23 Table 3.1. Species of Armillaria found in North America (Volk 2013). 69 24 Table 3.2. Number of plots sampled by habitat type on the Flagstaff 70 25 Ranger District, Coconino National Forest and the North Kaibab and 26 Williams Ranger Districts, Kaibab National Forest. 27 Table 3.3. Composition of plots by climate characterization associated 71 viii 1 with each habitat type as well as the proportion of plots identified as 2 containing Armillaria infection. 3 Table 3.4 a,b,c. The number of trees sampled, number of trees found with 72 4 Armillaria root disease, and the number of Armillaria samples 5 collected by habitat type on Flagstaff Ranger District, Coconino 6 National Forest and the North Kaibab and Williams Ranger Districts, 7 Kaibab National Forest.