DOCSLIB.ORG

Susceptibility of First Instar Toxorhynchites Splendensto Malathion, Naled and Resmethrin

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Susceptibility of First Instar Toxorhynchites Splendensto Malathion, Naled and Resmethrin CORE Metadata, citation and similar papers at core.ac.uk Provided by Biodiversity Heritage Library OAI Repository MARCH 1993 OpnRetroNlr, lrio ScreNrrrrc NorES o7 SUSCEPTIBILITY OF FIRST INSTAR TOXORHYNCHITES SPLENDENSTO MALATHION, NALED AND RESMETHRIN NOOR S. TIETZE, ERIC T. SCHREIBER, PHILIP G. HESTER, CHARLES F. HALLMON, MARY ANN OLSON AND KENNETH R. SHAFFER Florida Agricultural and Mechanical Uniuersity, John A. Mulrennan Sr. ResearchLaboratory, 4000 Franhford Auenue,Panama City, FL 32405 ABSTRACT. Acute toxicity tests were conductedto measurethe responseof first instar Toxorhyn- chitessplendens to commonly used mosquito adulticides: malathion, naled and resmethrin. The concen- trations of pesticidecausing 50% mortality (LCso)after 24 h was 2.87,69.1and 623ppb for resmethrin, malathionand naled,respectively. Naled was determined to be the leasttoxic of the 3 compoundstested for integrated use with Tx. splendens.The latter assessmentswere based on comparisons between laboratory-deriveddose-response curves and maximum concentrations reachedin standing water calcu- lated using standard application rates. Interest in using Toxorhynchites mosquitoes acute toxicity of 3 mosquitoadulticides to im- as biological control agentshas hinged upon the mature Tx. spl,endens. possibilityof reducingpopulations of container- The Toxorhynchites splendens used were developingmosquitoes. In the proper setting, rearedat the John A. Mulrennan Sr. Research Toxorhynchitesspecies may be efficacious con- Laboratory (JAMSRL), but were originally ob- trol agentsfor mosquitoes(Focks 1985, Focks et tained from the New Orleans Mosquito Control al. 1986). Toxorhynchitesamboinensis (Theo- District, New Orleans,Louisiana. Colony main- bald) was successfullyemployed for integrated tenance was describedin Focks and Boston control of Aedesaegypti (Linn.) in New Orleans (1979). Eggs were oviposited by Tx. splendens in conjunctionwith ULV malathion treatments into black cups (150 ml) and upon hatching, (Fockset al. 1986).Other species,such as ?r. individually placedinto 10 ml cells on a plastic splendens(Wiedemann), have receivedinterest multicell sheet to avoid injury or loss due to for use in low-incomeareas of Florida (E. T. cannibalism. Schreiber, unpublished data). Toxorhynchites Bioassayswere conducted in accordancewith spLendensis of particular interest due to its the AmericanSociety for Testing and Materials preferenceto oviposit into ground-levelcon- (ASTM 1980).The compoundstested were mal- tainers (Yap and Foo 1984), as typically found athion (Cythion@),naled (Dibrom@14) and res- in urban environments. methrin (Scourge@:18% resmethrin + 54% pi- As true for many biological control agents, peronylbutoxide). Standard methods and equip- Toxorhynchites-inducedreductions of pestifer- ment developedat JAMSRL (Tietzeet al. 1991) ous mosquitoesare not expectedto reach gbVo, were employed for the conduct of bioassays. but insteadare basedon establishmentof equi- Deviations from these methods are described Iibrium betweenpredator and prey (Focks1985). below. Larvae were singly transferred from the For this reason, Toxorhynchites must be inte- cellsinto 50-ml Pyrex beakerscontaining 40 ml grated into existing mosquito abatementpro- of aged well water. Each test consistedof 30 gramsas demonstratedby Focks et al. (1986). beakersper concentrationand 6 or 7 concentra- However, before further integration is at- tions in addition to controls. The results of 3 "valid" tempted, an evaluation of this Toxorhynchite tests (i.e., control mortality < 5%; dis- predator'ssusceptibility to commonlyused tox- solvedoxygen > 40%; and chi-squaretest for icants is warranted. This study investigatesthe homogeneityresult in P > 0.01),were combined Table 1. Toxicity of mosquito adulticides to <24h old Toxorhynchites splendens. Lethal concentration ng Test Allml length No. Compound (hours) tests LCuo 95%CL Malathion 243 69.1 63.2-76.5 (CWhion@) 483 49.8 44.8-55.9 Naled 243 623 554-696 (Dibrom* 14) 483 488 431-548 Resmethrin 243 2.87 2.65-3.15 (Scourge@) 483 2.07 7.95-2.20 98 JouRNer,oF THEArranRrcax Moseurro CourRor,Assocrerrorl Vol. 9, No. 1 100 were based on maximum labeled ground and MALATHION aerialapplication rates (volume per acre)assum- EO ing700%of the materialdeposited on water 1b.2 cm (6 in) in depth.In contrastto malathionand 60 naled,labeled rates for groundand aerial appli- cation of resmethrin were the same, These 40 worst-case-scenarioconcentrations predicted for ground and aerial ultra-low volume sprays indicated resmethrin and malathion to exceed 20 levels deemedtoxic to Tx. splendens(Fig. 1). This was not the case for naled, where both theoretical exposureswere below the dose-re- I ooo sponsecurve (Fig. 1). Naled quickly hydrolyzes 100 in water (Chen 1984)which may be the reason NALED for its relatively low toxicity there. (1990) 80 Rawlinsand Ragoonansingh compared t the susceptibilities of fourth instar Tx. ntocte- zuma (Dyar and Knab) and.Ae. aegyptito organ- F 60 ophosphates.They found that, while Tx. moc- tezurnawas more tolerant to temephosthan Ae. 40 CE aegypti,both specieshad similar susceptibilities o to malathion,fenthion, fenitrothion and chlor- = 20 pyrifos. They concludedthat temephoswas the only organophosphatetested that would be use- ll ful in an integrated management program '| 100 ooo 10000 againstthese species.In comparisonto the re- ported 100 value for Tx. moctezumo,we found the toxicity of malathion to Tx. splendensto be 5x RESMETH RIN greater (Table 1). This discrepancymay largely 80 be causedby differencesin larval instars tested (i.e., fourth vs. first instars), which is directly 60 relatedto differencesin larval sizeand esterase content (M. S. Mulla, unpublisheddata) or due 40 to speciesdifferences. Further testing in the field is warranted to assess?.r. spLendensmortality in their natural 20 tl habitat. Such studies should focus on whether $], field populations of larval and adult Tx. splen- dens arc affected by these adulticides.These t0 studies should simulate operational application techniques in typical urban environments and coNcENTRATIoN(ppb) determine what concentration of adulticide in Fig. 1. Dose-response curves of first instar ?oror- water results from such applications. hynchites splendens to adulticides, malathion, naled The authors thank William Turner and An- and resmethrin after 24 and 48 h of exposure. Arrows geleaLopez at the John A. Mulrennan Sr. Re- denote theoretical concentrations in 15.2 cm of water searchLaboratory for their participation in this based on maximum ground application rate (solid study. We also thank in-house and outside re- arrow) and maximum aerial application rate (open viewersfor their helpful comments. arrow). Theoretical aerial and ground rates for res- methrin are equal. REFERENCES CITED and analyzedusing probit and chi-squaretests Abbott, W. S. 1925. A method for computing the percent effectivenessof an insecticide.J. Econ. Entomol. to determineLCao and mortality. Mor- 18:265-267. talities were corrected using Abbott's formula ASTM. 1980. practice (Abbott Standard for conducting acute 1925). toxicity tests with fishes, macroinvertebrates,and Resmethrin was the most toxic compound to amphibians,pp. 7-24.In: Annual book of ASTM first instar Tx. spLendens,followed by malathion standards.Am. Soc.for Testingand Materials,Phil- and naled (Table 1). Theoreticalconcentrations adelphia,PA. MARcH 1993 OpnRlrroulr, lNo Sclpxttuc NorEs 99 Chen,Y. S. 1984.Metabolism and breakdownproducts Caribbeanpopulations of Aedesaegypti and Toxor- of Dibrom 14.J. Fla. Anti-Mosq.Assoc. 55:46-47. hynchitesnxoctezutna. J. Am. Mosq. Control Assoc. Focks,D. A. and M. D. Boston. 1979.A quantified 6:315-317. mass rearing technique for Toxorhynchites rutilus SAS Institute Inc. 1985. SAS user's guide: statistics. rutilus (Coquillett).Mosq. News 39:616-619. Cary,NC. Focks, D. A. 1985. Toxorhynchites,pp. 42-45. In: H. Sokal. R. R. and F. J. Rohlf. 1981.Biometry. W. H. C. Chapman(ed.), Biological control of mosquitoes. Freemanand Company, San Francisco,CA. Bull. Am. Mosq.Control Assoc.6. Tietze,N. S.,P. G. Hester,C. F. Hallmon,M. A. Olson Focks,D. A., S. R. Sackett,K. O. Kloter, D. A. Dame and K. R. Shaffer. 1991.Acute toxicity of mosqui- and G. T. Carmichael. 1986.The integrated use of tocidal compoundsto young mosquitofish, Gambu- Toxorhynchites amboinensisand ground-level ULV siaaffinis. J. Am. Mosq.Control Assoc.7:290-293. insecticide application to suppress Aedes aegypti Yap, H. H. and A. E. S. Foo. 1984.Laboratory studies (Diptera:Culicidae). J. Med. Entomol. 23:513-519. on the oviposition site preferenceof Toxorhynchites Rawlins,S. C. and R. Ragoonansingh.1990. Compar- splendens (Diptera: Culicidae). J. Med Entomol. ative organophosphorusinsecticide susceptibility in 21:183-187..
Load more

Recommended publications
  • The Introduction of Toxorhynchites Brevipalpis Theobald Into the Purpose of This Brief Paper Is to Place on Record an Account Of
    Vol. XIV, No. 2, March, 1951 237 The Introduction of Toxorhynchites brevipalpis Theobald into the Territory of Hawaii By DAVID D. BONNET MEDICAL ENTOMOLOGIST, DEPARTMENT OF HEALTH, HONOLULU and STEPHEN M. K. HU CHIEF, BUREAU OF MOSQUITO CONTROL, HONOLULU The purpose of this brief paper is to place on record an account of the introduction of the South African mosquito Toxorhynchites (= Megarhinus) * brevipalpis Theobald into the Territory of Hawaii. The importation of this insect and its establishment would, it is believed, aid in the control of the forest day mosquito, Aedes albopictus Skuse. Any type of container, whether it be a natural container such as a tree hole, bamboo stump, pineapple lily (Bilbergia sp.) or rock hole; or an artificial container such as a vine bowl, tin can, tub, auto tire or bottle, when filled with water, can serve as a breeding location for Aedes albopictus. This varied breeding habit complicates control as it neces sitates the treatment or elimination of a multitude of large and small water-holding containers. Adequate control has been obtained in the urban areas of Hawaii by premises-to-premises inspection, and house holder cooperation, following usual Aedes aegypti (L.) control pro cedures. There are, however, large areas outside the cities where such control measures are not feasible. These areas are principally mountain forest regions which contain a sufficient number of natural breeding situations to maintain a large mosquito population. This population of Aedes albopictus serves not only as a health menace and as a reservoir for reinvasion, but also discourages extensive use of many fine recreation areas.
    [Show full text]
  • Table of Contents
    Table of Contents Oral Presentation Abstracts ............................................................................................................................... 3 Plenary Session ............................................................................................................................................ 3 Adult Control I ............................................................................................................................................ 3 Mosquito Lightning Symposium ...................................................................................................................... 5 Student Paper Competition I .......................................................................................................................... 9 Post Regulatory approval SIT adoption ......................................................................................................... 10 16th Arthropod Vector Highlights Symposium ................................................................................................ 11 Adult Control II .......................................................................................................................................... 11 Management .............................................................................................................................................. 14 Student Paper Competition II ...................................................................................................................... 17 Trustee/Commissioner
    [Show full text]
  • A Review of the Mosquito Species (Diptera: Culicidae) of Bangladesh Seth R
    Irish et al. Parasites & Vectors (2016) 9:559 DOI 10.1186/s13071-016-1848-z RESEARCH Open Access A review of the mosquito species (Diptera: Culicidae) of Bangladesh Seth R. Irish1*, Hasan Mohammad Al-Amin2, Mohammad Shafiul Alam2 and Ralph E. Harbach3 Abstract Background: Diseases caused by mosquito-borne pathogens remain an important source of morbidity and mortality in Bangladesh. To better control the vectors that transmit the agents of disease, and hence the diseases they cause, and to appreciate the diversity of the family Culicidae, it is important to have an up-to-date list of the species present in the country. Original records were collected from a literature review to compile a list of the species recorded in Bangladesh. Results: Records for 123 species were collected, although some species had only a single record. This is an increase of ten species over the most recent complete list, compiled nearly 30 years ago. Collection records of three additional species are included here: Anopheles pseudowillmori, Armigeres malayi and Mimomyia luzonensis. Conclusions: While this work constitutes the most complete list of mosquito species collected in Bangladesh, further work is needed to refine this list and understand the distributions of those species within the country. Improved morphological and molecular methods of identification will allow the refinement of this list in years to come. Keywords: Species list, Mosquitoes, Bangladesh, Culicidae Background separation of Pakistan and India in 1947, Aslamkhan [11] Several diseases in Bangladesh are caused by mosquito- published checklists for mosquito species, indicating which borne pathogens. Malaria remains an important cause of were found in East Pakistan (Bangladesh).
    [Show full text]
  • F. Christian Thompson Neal L. Evenhuis and Curtis W. Sabrosky Bibliography of the Family-Group Names of Diptera
    F. Christian Thompson Neal L. Evenhuis and Curtis W. Sabrosky Bibliography of the Family-Group Names of Diptera Bibliography Thompson, F. C, Evenhuis, N. L. & Sabrosky, C. W. The following bibliography gives full references to 2,982 works cited in the catalog as well as additional ones cited within the bibliography. A concerted effort was made to examine as many of the cited references as possible in order to ensure accurate citation of authorship, date, title, and pagination. References are listed alphabetically by author and chronologically for multiple articles with the same authorship. In cases where more than one article was published by an author(s) in a particular year, a suffix letter follows the year (letters are listed alphabetically according to publication chronology). Authors' names: Names of authors are cited in the bibliography the same as they are in the text for proper association of literature citations with entries in the catalog. Because of the differing treatments of names, especially those containing articles such as "de," "del," "van," "Le," etc., these names are cross-indexed in the bibliography under the various ways in which they may be treated elsewhere. For Russian and other names in Cyrillic and other non-Latin character sets, we follow the spelling used by the authors themselves. Dates of publication: Dating of these works was obtained through various methods in order to obtain as accurate a date of publication as possible for purposes of priority in nomenclature. Dates found in the original works or by outside evidence are placed in brackets after the literature citation.
    [Show full text]
  • Toxorhynchites Species: a Review of Current Knowledge
    insects Review Toxorhynchites Species: A Review of Current Knowledge Claire L. Donald 1,2,* , Padet Siriyasatien 3 and Alain Kohl 2 1 Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK 2 MRC-University of Glasgow Centre for Virus Research, Glasgow G61 1QH, Scotland, UK; [email protected] 3 Vector Biology and Vector Borne Disease Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand; [email protected] * Correspondence: [email protected]; Tel.: +44-141-330-5263 Received: 10 September 2020; Accepted: 28 October 2020; Published: 30 October 2020 Simple Summary: Mosquitoes are well known to spread diseases when they take a blood meal. However, not all species feed on blood but instead get their nourishment from other sources. One such species is Toxorhynchites, which are a paradox among mosquitoes. These mosquitoes are entirely non-blood feeding and, as a result, are not considered to be harmful to human health. Indeed, since their larvae feed on the larvae of pest species and other aquatic insects, they are a potential counter measure against the spread of mosquito-transmitted diseases. Their effective application has been hampered due to a lack of understanding and inconsistencies in their descriptions. This review aims to build upon previously published information and summarize recent findings to support their use in combating mosquito-transmitted infections. Abstract: The increasing global incidence of mosquito-borne infections is driving a need for effective control methods. Vector populations have expanded their geographical ranges, while increasing resistance to chemical insecticides and a lack of effective treatments or vaccines has meant that the development of vector control methods is essential in the fight against mosquito-transmitted diseases.
    [Show full text]
  • Egg Cannibalism and Carnivory Among Three
    Ecc CeNNrsnlrsu rN ?oxoRnyNcHITEs 359 EGG CANNIBALISM AND CARNIVORYAMONG THREE SPECIESOF TOXORHYNCHITES J. R. LINLEY exo D. DUZAK Florida Medical Entomolngy Laboratory, Institute of Food and.Agricultural Sciences,Uniuersity of Flnrida, Vero Beach, FL 32962 ABSTRACT. In laboratory experiments, third and early fourth instar larvae of Toxorhynchites arnboinensis,Tr. splendensand Tx. breuipalpis,previouslystarved 24 h, rapidly cannibalizedeggs of their own species, or ate the eggs of other species present on the water surface in small containers. Toxorhynchitesamboinensis and, Tx. splendenslarvae of either instar cannibalizedeggs somewhat more rapidly than Tx. breuipalpis,probably becatse brevipalpiseggs distributed themselvesaround the edgeof the container and were less accessible.When offered heterospecificeggs, fourth instar larvae of all three speciesate them as efficiently as they cannibalized their own except that eggsof Tr. breuipalpiswerc eaten very slowly. Toxorhyrchites atnboinensrslarvae were offered conspecific hatched eggs,and these also were consumed. INTRODUCTION 12:12.Lawae were reared throughout develop- ment in the presence of an excess of Aedes (Linley In a recent study 1988) of oviposition aegypti (Linn.) prey of the same instar. Adults (Doles- behavior in Toxorhynchitesamboinensis emerged in cages 38 x 50 x 65 cm in which chall), it was observedthat when females laid mating took place and eggs were obtained as their eggson water containing 20 third or fourth needed by allowing the females to oviposit in instar Iarvaepreviously starved 24 h, large num- black, plastic pots 9 cm diam x 7 cm deep bers of eggs were cannibalized. Although this containing tap water. was apparently the first published report of egg All experiments were done in a laboratory cannibalism in Toxorhynchites, egg carnivory maintainedat27.0 + 2.0'C under approximately (Kazana had been reported previously et al.
    [Show full text]
  • J. Aer. Mosq. Conrnol Assoc. TOXORHYNCHITES MOCTEZUMA
    376 J. Aer. Mosq. CoNrnol Assoc. Vol. l, No. 3 cently ejected the virus while feeding on a car- lated from culicine mosquitoes in the Union of bohydrate source and the virus has yet to be South Africa. S. Afr. Med. J. 30:631-636. replenished into the salivary duct, and (3) W.H.O. 1972. Vector Ecology. Tech. Rep. Ser. "salivary there might be a gland escape bar- 50 I : l-38. rier" which prevents virus leaving these glands. This would be the situation when the produc- tion of infectious virus has been modulated to low levels. Such an escape barrier could explain TOXORHYNCHITES MOCTEZUMA, A the lowered transmission rates which have beerr POTENTIAL BIOLOGICAL CONTROL obtained after prolonged incubation periods AGENT IN TRINIDAD AND (Mclntosh and 1970, Mangiafico l97l). Jupp TOBAGO, W. I.1 I wish to thank the Director-General of National Health and Welfare, Pretoria. for DAVED. CHADEE' permission to publish. Ministry of Health and Environment, Insect Vector Control Division, f3 Queen Street, St. Joseph, Trinidad, West Indies ReferencesCited Toxorhynchitesmoctezuma (Dyar and Knab) fas Aitken, T. H. G. 1977. An in vitro feeding technique theobaldi (Dyar and Knab) in Knight and Stone for artificially demonstrating virus transmission by (1977)l has a wide distribution from Mexico in mosquitoes. Mosq. News 37:l3G- 133. Central America to Venezuela in South Chamberlain, R. W. 1968. Arboviruses, the America (Rubio et al. 1980; S. J. Heinemann, arthropod-borne animal viruses. Current Topics personal communication). In the Caribbean Microbiol. Immunol. 42:3U58. Basin, it has a limited distribution and is found Clarke, D.
    [Show full text]
  • T3-B1-Mosquitoecology.Pdf
    Suffolk County Vector Control and Wetlands Management Long-Term Plan Literature Review Task Three – Book 1 -- Long Island Mosquitoes October 2004 SUFFOLK COUNTY LONG TERM PLAN The Consultant Team Cashin, Associates, P.C. Hauppauge, NY Subconsultants Cameron Engineering, L.L.P. Syosset, NY Integral Consulting Annapolis, MD Bowne Management Systems, Inc. Mineola, NY Kamazima Lwiza, PhD University at Stony Brook, NY Ducks Unlimited Stony Brook, NY Steven Goodbred, PhD & Laboratory University at Stony Brook, NY RTP Environmental Westbury, NY Sinnreich, Safar & Kosakoff Central Islip, NY Bruce Brownawell, PhD & Laboratory University at Stony Brook, NY Anne McElroy, PhD & Laboratory University at Stony Brook, NY Andrew Spielman, PhD Harvard School of Public Health, Boston, MA Richard Pollack, PhD Harvard School of Public Health, Boston, MA Wayne Crans, PhD Rutgers University, New Brunswick, NJ Susan Teitelbaum, PhD Mount Sinai School of Medicine, NY Zawicki Vector Management Consultants Freehold, NJ Robert Turner, PhD & Laboratory Southampton College, NY Christopher Gobler, PhD & Laboratory Southampton College, NY Jerome Goddard, PhD Mississippi Department of Health, Jackson, MS Sergio Sanudo, PhD & Laboratory University of Stony Brook, NY Suffolk County Department of Health Hauppauge, NY Services, Division of Environmental Quality Project Management Richard LaValle, P.E., Chief Deputy Suffolk County Department of Public Works, Commissioner Yaphank, NY Vito Minei, P.E., Director, Division of Suffolk County Department of Health Services, Environmental Quality Hauppauge, NY Walter Dawydiak, Jr., P.E., J.D., Chief Suffolk County Department of Health Services, Engineer, Division of Environmental Hauppauge, NY Quality Dominick Ninivaggi, Superintendent, Suffolk County Department of Public Works, Division of Vector Control Yaphank, NY Cashin Associates, P.C.
    [Show full text]
  • Mosquitopia: the Place of Pests in a Healthy World / Edited by Marcus Hall and Dan Tamir
    MOSQUITOPIA This edited volume brings together natural scientists, social scientists and humanists to assess if (or how) we may begin to coexist harmoniously with the mosquito. The mosquito is humanity’s deadliest animal, killing over a million people each year by transmitting malaria, yellow fever, Zika and several other diseases. Yet of the 3,500 species of mosquito on Earth, only a few dozen of them are really dangerous—so that the question arises as to whether humans and their mosquito foe can learn to live peacefully with one another. Chapters assess polarizing arguments for conserving and preserving mosquitoes, as well as for controlling and killing them, elaborating on possible consequences of both strategies. This book provides informed answers to the dual question: could we eliminate mosquitoes, and should we? Offering insights spanning the technical to the philosophical, this is the “go to” book for exploring humanity’s many relationships with the mosquito—which becomes a journey to finding better ways to inhabit the natural world. Mosquitopia will be of interest to anyone wanting to explore dependencies between human health and natural systems, while offering novel perspectives to health planners, medical experts, environmentalists and animal rights advocates. Marcus Hall is an environmental historian and professor at the University of Zurich. In exploring changing human relationships with the natural world, Hall has turned to such subjects as restoring, rewilding, invasive species, warfare, earth art, chronobi- ology, malaria, and parasites. His books include Earth Repair, Restoration and History, Crossing Mountains, and (with Marco Armiero) Nature and History in Modern Italy. Dan Tamïr is environmental historian and research associate at the University of Zurich.
    [Show full text]
  • Belkin Et Al 1965.Pdf
    FkmchasedbyUnitedStat~B~*~&f of~gricultureforOfficl~us8.; ~~~otlfrihutions of -the “: 744 American Entomo/ogica!_: Institute > Volume 1, Number-5, 1965 _ . .- . ,“ . e ._ MOSQUITO STUDIES (Dipte@g -Culfcidae) V. Mosquitoes originally descr from Middle America. By John N. Belkin, Robert X. &chick, and Sandra J. Heinemzuxn. :. PUBLICATIONS c&the AMBRICAN ENTOMOLOGICALINSTITUTE ContributionSeries The Contributionsare for larger papers on insects. Each paper is a s e number, with separate paginationand index. Separatenumbers aggregating about 506 constitutea volume. Issues appear irregularly, as suitabie manuscriptsare available. i t Copiesare sold separately or in subscriptionsto completevolumes. C mplete volumes are $12.00. The price of separate numbersvaries. Subscribersare billed f Lr each volume with its beginningnumber, and receive the parts as issued. Orders for separate mbers that total less than $8.00 must be accompaniedby payment. Address orders OPcurres ondence to the American EntomologicalInstitute, 5950 Warren Road, Ann Arbor, Mich@anr ,8105, U.S. A. i -L-issued to date: I Volume I No. 1. Dasch, Clement E. 1964. The NeotropicDiplaxontinae (Byrne L era, Ichneumoni- ’ ’ dae). 75 pagea, 69 figures. Price: $2.25, postpaid No. 2. Belltin, JohnN., et al. 1965. A project for a systematic&I& of the mosquitoesof Middle America, - Belkin, JohnN., et al. 1965. Methodsfor the collection, rearing and preservation of mosquitoes.-- 78 pages, 4 figures. Price: $2.25, postpaid Nu. 3. In preparation. No. 4. Ramalingam, Shivaji, and JohnN. Belkln. 1965. Two new Aedeefro& Tongaand Samoa. Belkin, JohnN. 1965. The mosquitoesof the Robinson-PeabodyMuseum of Salem expeditionto the southwestPacific, 1966. 6 34 pages, 3 figures. Price: $1.00, postpaid No.
    [Show full text]
  • Official Lists and Indexes of Names and Works in Zoology
    OFFICIAL LISTS AND INDEXES OF NAMES AND WORKS IN ZOOLOGY Supplement 1986-2000 Edited by J. D. D. SMITH Copyright International Trust for Zoological Nomenclature 2001 ISBN 0 85301 007 2 Published by The International Trust for Zoological Nomenclature c/o The Natural History Museum Cromwell Road London SW7 5BD U.K. on behalf of lICZtN] The International Commission on Zoological Nomenclature 2001 STATUS OF ENTRIES ON OFFICIAL LISTS AND INDEXES OFFICIAL LISTS The status of names, nomenclatural acts and works entered in an Official List is regulated by Article 80.6 of the International Code of Zoological Nomenclature. All names on Official Lists are available and they may be used as valid, subject to the provisions of the Code and to any conditions recorded in the relevant entries on the Official List or in the rulings recorded in the Opinions or Directions which relate to those entries. However, if a name on an Official List is given a different status by an adopted Part of the List of Available Names in Zoology the status in the latter is to be taken as correct (Article 80.8). A name or nomenclatural act occurring in a work entered in the Official List of Works Approved as Available for Zoological Nomenclature is subject to the provisions of the Code, and to any limitations which may have been imposed by the Commission on the use of that work in zoological nomenclature. OFFICIAL INDEXES The status of names, nomenclatural acts and works entered in an Official Index is regulated by Article 80.7 of the Code.
    [Show full text]
  • Research on the Mosquito Subfamily Toxorhynchitinae (Diptera: Culicidae) Ii-Description of the Afrotropical Subgenus Afrorhynchus Subgen
    DECEMBER 1991 195 RESEARCH ON THE MOSQUITO SUBFAMILY TOXORHYNCHITINAE (DIPTERA: CULICIDAE) II-DESCRIPTION OF THE AFROTROPICAL SUBGENUS AFRORHYNCHUS SUBGEN. NOV?v2 H. RIBEIRO Instituto de Higiene e Medicina Tropical, UniversidadeNova de Lisboa, Rua da Junqueira, 96-100, 1300 Lisboa, Portugal ABSTRACT. The lutescensgroup of Edwardsis removed from the subgenusToxorhynchites and given subgenericrank. Four subgeneraare now recognized within the genus Toxorhyn- chites: Ankylorhynchus and Lynchiella, Neotropical; Toxorhynchites,Gondwanian, repre- sented in the Afrotropical Region by the brevipalpis group of Edwards; and Afrorhynchus subgen. nov., purely Afrotropical. The new taxon, typified by Toxorhynchiteslutescens, is describedand a key to all four subgenerais provided. INTRODUCTION and, along with the Oriental christophigroup, may be consideredas one of the three groups The mosquito family Culicidae (Diptera) of the splendensseries (Steffan and Evenhuis includes the three subfamilies Anophelinae, 1985). The lutescensgroup, on the contrary, Culicinae and Toxorhynchitinae (Edwards exhibits charactersthat clearly separateit not 1932). The latter, containing only the genus only from the brevipalpisgroup but also from ToxorhynchitesTheobald, haspredacious lar- all the other groupswithin the subgenusTox- vae which are potential agents of biocontrol orhynchites, as well as from the subgenera of other mosquitoes (Steffan and Evenhuis Ankylorhynchusand Lynchiella. Accordingly, 1981). Within the genus Toxorhynchites,the the lutescens group is removed
    [Show full text]