Platyhelminthes-Turbellaria Checklist (Generated Tue Feb 12 19:11:10 +0100 2013 from FADA Website)
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Integrative Descriptions of Two New Species of Dugesia from Hainan Island, China (Platyhelminthes, Tricladida, Dugesiidae)
ZooKeys 1028: 1–28 (2021) A peer-reviewed open-access journal doi: 10.3897/zookeys.1028.60838 RESEARCH ARTICLE https://zookeys.pensoft.net Launched to accelerate biodiversity research Integrative descriptions of two new species of Dugesia from Hainan Island, China (Platyhelminthes, Tricladida, Dugesiidae) Lei Wang1,3, Zi-mei Dong1, Guang-wen Chen1, Ronald Sluys2, De-zeng Liu1 1 College of Life Science, Henan Normal University, Xinxiang, 453007 Henan, China 2 Naturalis Biodiver- sity Center, Leiden, the Netherlands 3 Medical College, Xinxiang University, Xinxiang 453003, China Corresponding author: Guang-wen Chen ([email protected]) Academic editor: Y. Mutafchiev | Received 17 November 2020 | Accepted 24 February 2021 | Published 05 April 2021 http://zoobank.org/A5EF1C8A-805B-4AAE-ACEB-C1CACB691FCA Citation: Wang L, Dong Z-m, Chen G-w, Sluys R, Liu D-z (2021) Integrative descriptions of two new species of Dugesia from Hainan Island, China (Platyhelminthes, Tricladida, Dugesiidae). ZooKeys 1028: 1–28. https://doi. org/10.3897/zookeys.1028.60838 Abstract Two new species of the genus Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from Hainan Island of China are described on the basis of morphological, karyological and molecular data. Dugesia semiglobosa Chen & Dong, sp. nov. is mainly characterized by a hemispherical, asymmetrical penis papilla with ven- trally displaced ejaculatory duct opening terminally at tip of penis papilla; vasa deferentia separately open- ing into mid-dorsal portion of intrabulbar seminal vesicle; two diaphragms in the ejaculatory duct; copula- tory bursa formed by expansion of bursal canal, lined with complex stratified epithelium, which projects through opening in bursa towards intestine, without having open communication with the gut; mixoploid chromosome complement diploid (2n = 16) and triploid (3n = 24), with metacentric chromosomes. -
S42003-018-0151-2.Pdf
ARTICLE DOI: 10.1038/s42003-018-0151-2 OPEN Coordination between binocular field and spontaneous self-motion specifies the efficiency of planarians’ photo-response orientation behavior Yoshitaro Akiyama1,2, Kiyokazu Agata1,3 & Takeshi Inoue 1,3 1234567890():,; Eyes show remarkable diversity in morphology among creatures. However, little is known about how morphological traits of eyes affect behaviors. Here, we investigate the mechan- isms responsible for the establishment of efficient photo-response orientation behavior using the planarian Dugesia japonica as a model. Our behavioral assays reveal the functional angle of the visual field and show that the binocular field formed by paired eyes in D. japonica has an impact on the accurate recognition of the direction of a light source. Furthermore, we find that the binocular field in coordination with spontaneous wigwag self-motion of the head specifies the efficiency of photo-responsive evasive behavior in planarians. Our findings suggest that the linkage between the architecture of the sensory organs and spontaneous self-motion is a platform that serves for efficient and adaptive outcomes of planarian and potentially other animal behaviors. 1 Department of Biophysics, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan. 2 Department of Advanced Interdisciplinary Studies, Graduate School of Engineering, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. 3 Department of Life Science, Faculty of Science, Gakushuin University, 1-5-1 -
A Comprehensive Comparison of Sex-Inducing Activity in Asexual
Nakagawa et al. Zoological Letters (2018) 4:14 https://doi.org/10.1186/s40851-018-0096-9 RESEARCH ARTICLE Open Access A comprehensive comparison of sex-inducing activity in asexual worms of the planarian Dugesia ryukyuensis: the crucial sex-inducing substance appears to be present in yolk glands in Tricladida Haruka Nakagawa1†, Kiyono Sekii1†, Takanobu Maezawa2, Makoto Kitamura3, Soichiro Miyashita1, Marina Abukawa1, Midori Matsumoto4 and Kazuya Kobayashi1* Abstract Background: Turbellarian species can post-embryonically produce germ line cells from pluripotent stem cells called neoblasts, which enables some of them to switch between an asexual and a sexual state in response to environmental changes. Certain low-molecular-weight compounds contained in sexually mature animals act as sex-inducing substances that trigger post-embryonic germ cell development in asexual worms of the freshwater planarian Dugesia ryukyuensis (Tricladida). These sex-inducing substances may provide clues to the molecular mechanism of this reproductive switch. However, limited information about these sex-inducing substances is available. Results: Our assay system based on feeding sex-inducing substances to asexual worms of D. ryukyuensis is useful for evaluating sex-inducing activity. We used the freshwater planarians D. ryukyuensis and Bdellocephala brunnea (Tricladida), land planarian Bipalium nobile (Tricladida), and marine flatworm Thysanozoon brocchii (Polycladida) as sources of the sex-inducing substances. Using an assay system, we showed that the three Tricladida species had sufficient sex-inducing activity to fully induce hermaphroditic reproductive organs in asexual worms of D. ryukyuensis. However, the sex-inducing activity of T. brocchii was sufficient only to induce a pair of ovaries. We found that yolk glands, which are found in Tricladida but not Polycladida, may contain the sex-inducing substance that can fully sexualize asexual worms of D. -
A Comprehensive Comparison of Sex-Inducing
Nakagawa et al. Zoological Letters (2018) 4:14 https://doi.org/10.1186/s40851-018-0096-9 RESEARCH ARTICLE Open Access A comprehensive comparison of sex-inducing activity in asexual worms of the planarian Dugesia ryukyuensis: the crucial sex-inducing substance appears to be present in yolk glands in Tricladida Haruka Nakagawa1†, Kiyono Sekii1†, Takanobu Maezawa2, Makoto Kitamura3, Soichiro Miyashita1, Marina Abukawa1, Midori Matsumoto4 and Kazuya Kobayashi1* Abstract Background: Turbellarian species can post-embryonically produce germ line cells from pluripotent stem cells called neoblasts, which enables some of them to switch between an asexual and a sexual state in response to environmental changes. Certain low-molecular-weight compounds contained in sexually mature animals act as sex-inducing substances that trigger post-embryonic germ cell development in asexual worms of the freshwater planarian Dugesia ryukyuensis (Tricladida). These sex-inducing substances may provide clues to the molecular mechanism of this reproductive switch. However, limited information about these sex-inducing substances is available. Results: Our assay system based on feeding sex-inducing substances to asexual worms of D. ryukyuensis is useful for evaluating sex-inducing activity. We used the freshwater planarians D. ryukyuensis and Bdellocephala brunnea (Tricladida), land planarian Bipalium nobile (Tricladida), and marine flatworm Thysanozoon brocchii (Polycladida) as sources of the sex-inducing substances. Using an assay system, we showed that the three Tricladida species had sufficient sex-inducing activity to fully induce hermaphroditic reproductive organs in asexual worms of D. ryukyuensis. However, the sex-inducing activity of T. brocchii was sufficient only to induce a pair of ovaries. We found that yolk glands, which are found in Tricladida but not Polycladida, may contain the sex-inducing substance that can fully sexualize asexual worms of D. -
Dugesia Japonica Is the Best Suited of Three Planarian Species for High-Throughput
bioRxiv preprint doi: https://doi.org/10.1101/2020.01.23.917047; this version posted January 24, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Dugesia japonica is the best suited of three planarian species for high-throughput 2 toxicology screening 3 Danielle Irelanda, Veronica Bocheneka, Daniel Chaikenb, Christina Rabelera, Sumi Onoeb, Ameet 4 Sonib, and Eva-Maria S. Collinsa,c* 5 6 a Department of Biology, Swarthmore College, Swarthmore, Pennsylvania, United States of 7 America 8 b Department of Computer Science, Swarthmore College, Swarthmore, Pennsylvania, United 9 States of America 10 c Department of Physics, University of California San Diego, La Jolla, California, United States of 11 America 12 13 14 15 16 * Corresponding author 17 Email: [email protected] (E-MSC) 18 Address: Martin Hall 202, 500 College Avenue, Swarthmore College, Swarthmore, PA 19081 19 Phone number: 610-690-5380 20 21 22 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.01.23.917047; this version posted January 24, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 23 Abstract 24 High-throughput screening (HTS) using new approach methods is revolutionizing 25 toxicology. Asexual freshwater planarians are a promising invertebrate model for neurotoxicity 26 HTS because their diverse behaviors can be used as quantitative readouts of neuronal function. -
UNIVERSITY of CALIFORNIA SAN DIEGO Establishment and Validation of the Freshwater Planarian, Dugesia Japonica, As an Alternative
UNIVERSITY OF CALIFORNIA SAN DIEGO Establishment and Validation of the Freshwater Planarian, Dugesia japonica, as an Alternative Animal Model for Developmental Neurotoxicology using Organophosphorus Pesticides A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Biology by Danielle Hagstrom Committee in charge: Professor Eva-Maria Schoetz Collins, Chair Professor James Posakony, Co-Chair Professor Palmer Taylor Professor Robert Tukey Professor Jing Wang Professor Deborah Yelon 2018 Copyright Danielle Hagstrom, 2018 All rights reserved. The Dissertation of Danielle Hagstrom is approved, and it is acceptable in quality and form for publication on microfilm and electronically: Co-Chair Chair University of California San Diego 2018 iii TABLE OF CONTENTS Signature Page………………………………………………………………………………... iii Table of Contents……………………………………………………………………………... iv List of Figures……………………………………………………………………………........ v List of Tables………………………………………………………………………………..... vii Acknowledgements………………………………………………………………………....... viii Vita…………………………………………………………………………………………… xii Abstract of the Dissertation…………………………………………………………………… xiii Chapter 1: Planarian brain regeneration as a model system for developmental neurotoxicology………….……………………………………………………………………. 1 Chapter 2: Freshwater planarians as an alternative animal model for neurotoxicology………. 38 Chapter 3: Multi-behavioral endpoint testing of an 87-chemical compound library in freshwater planarians………………………………………………………………………………………. 83 Chapter 4: Comparative -
Title Genome Analysis of the Planarian Dugesia Japonica( Dissertation
Genome analysis of the planarian Dugesia japonica( Title Dissertation_全文 ) Author(s) An, Yang Citation 京都大学 Issue Date 2015-03-23 URL https://doi.org/10.14989/doctor.k18831 Right 許諾条件により本文は2016/03/23に公開 Type Thesis or Dissertation Textversion ETD Kyoto University Genome analysis of the planarian Dugesia japonica APPROVED BY SUPERVISING COMMITTEE: Supervisor: Kiyokazu AGATA Takeshi INOUE Genome analysis of the planarian Dugesia japonica by Yang AN Thesis Presented to the Faculty of the Graduate School of Science Kyoto University in Fulfillment of the Requirements for the Degree of Doctor of Science Kyoto University January 2015 Table of Contents CHAPTER 1 ...............................................................................................................1 1.1. PLANARIANS ...................................................................................................1 1.2. THE ORIGIN OF PLANARIAN RESEARCH BEFORE THE MOLECULAR BIOLOGY AGE – A LONG HISTORY .................................................................3 1.3. D. JAPONICA IS AN EXCELLENT MODEL PLANARIAN IN THE AGE OF MOLECULAR BIOLOGY ..................................................................................7 1.4. GENOME IS INDISPENSABLE FOR FURTHER STUDY OF D. JAPONICA IN THE GENOMICS ERA ..............................................................................................9 1.5. PROJECT AIMS ..............................................................................................11 CHAPTER 2. ............................................................................................................12 -
Draft Genome of Dugesia Japonica Provides Insights Into Conserved
An et al. Zoological Letters (2018) 4:24 https://doi.org/10.1186/s40851-018-0102-2 RESEARCHARTICLE Open Access Draft genome of Dugesia japonica provides insights into conserved regulatory elements of the brain restriction gene nou-darake in planarians Yang An1,9, Akane Kawaguchi2,10, Chen Zhao3,9,13, Atsushi Toyoda4, Ali Sharifi-Zarchi5,6,7, Seyed Ahmad Mousavi5, Reza Bagherzadeh1,6,8,11, Takeshi Inoue1,11, Hajime Ogino2,12, Asao Fujiyama4, Hamidreza Chitsaz5, Hossein Baharvand6,8 and Kiyokazu Agata1,11* Abstract Background: Planarians are non-parasitic Platyhelminthes (flatworms) famous for their regeneration ability and for having a well-organized brain. Dugesia japonica is a typical planarian species that is widely distributed in the East Asia. Extensive cellular and molecular experimental methods have been developed to identify the functions of thousands of genes in this species, making this planarian a good experimental model for regeneration biology and neurobiology. However, no genome-level information is available for D. japonica, and few gene regulatory networks have been identified thus far. Results: To obtain whole-genome information on this species and to study its gene regulatory networks, we extracted genomic DNA from 200 planarians derived from a laboratory-bred asexual clonal strain, and sequenced 476 Gb of data by second-generation sequencing. Kmer frequency graphing and fosmid sequence analysis indicated a complex genome that would be difficult to assemble using second-generation sequencing short reads. To address this challenge, we developed a new assembly strategy and improved the de novo genome assembly, producing a 1.56 Gb genome sequence (DjGenome ver1.0, including 202,925 scaffolds and N50 length 27,741 bp) that covers 99.4% of all 19,543 genes in the assembled transcriptome, although the genome is fragmented as 80% of the genome consists of repeated sequences (genomic frequency ≥ 2). -
Morphological Changes of Gonad and Gene Expression Patterns During Desexualization in Dugesia Japonica (Platyhelminthes: Dugesiidae)
ZOOLOGIA 35: e21933 ISSN 1984-4689 (online) zoologia.pensoft.net RESEARCH ARTICLE Morphological changes of gonad and gene expression patterns during desexualization in Dugesia japonica (Platyhelminthes: Dugesiidae) Zimei Dong 1, Changying Shi 1 , Gengbo Chu 1, Yanping Dong 1, Guangwen Chen 1, Dezeng Liu 1 1College of Life Science, Henan Normal University. Xinxiang, 453007 Henan, China. Corresponding author: Guangwen Chen ([email protected]) http://zoobank.org/B50FF002-873C-491A-9206-0FF6878DA853 ABSTRACT. Planarians, the representatives of an ancient bilaterian group with complex reproductive system and high re- generative capabilities, are model system suitable for studying the basic molecular requirements for the development of the reproductive system. To further explore the morphological changes of the gonads during desexualization and the molecular events of the genes controlling the reproductive system development in planarians, we have investigated the histological changes of ovary and testis by paraffin section and the expression patterns of reproductive-related genes by the quantitative real-time PCR in Dugesia japonica Ichikawa & Kawakatsu, 1964, upon starvation. The four genes, Djprps, DjvlgA, DjvlgB and Djnos, have been selected. The research results show that the degradation of ovary changes from outside layer to inside, and the testis changes are opposite; the reproductive capacity of the planarians starts to be damaged from the 17th to 25th days and to disappear completely from the 26th to 37th days during starvation. The expression patterns of the four genes exhibit the obvious dynamic variations during their desexualization, which indicates that these genes might be involved in gonad development. KEY WORDS. Planarian, food deprivation, germ cells, reproductive system. -
A List of Nominal Taxa of Planarians and Nemertean Described by Kawakatsu and His Coauthors
Kawakatsu’s Web Library on Planarians: August 20, 2009. A List of Nominal Taxa of Planarians and Nemertean Described by Kawakatsu and His Coauthors By Masaharu KAWAKATSU Introduction This bibliographic web article is a continuation from Kawakatsu’s previous web article entitled “Short Reminiscences of a Turbellariologist --- At the Occasion of His 79th Birthday” (2008). http://victoriver.com . Left button: Reminiscences (see p. 14). Kawakatsu made a start in the taxonomic study of planarians in 1961 after he moved to Fuji Women’s College, Sapporo in Hokkaidô, Japan. During the period 1962 to the end of the 1990s’, Kawakatsu and his coauthors published various taxonomic papers including original descriptions of freshwater and land planarians (i.e., so-called “Tricladida Paludicola” and “Tricladida Terricola”). After 1987, serial taxonomic and bibliographic papers on land planarians have been published by Ogren & Kawakatsu. The <Land Planarian Indices Series> contains taxonomic and geographical distributional records of every known species of this animal group in the world. Dr. Eudóxia M. Froehlich (after 1992) and Dr. Hugh D. Jones (after 2003) have kindly joined us. Although Dr. Ogren died in the year of 2005, 27 parts of this series were published until the end of 2008 (cf. Kawakatsu, Wu, Sluys, Sasaki, Kawakatsu (M-y.) & Kawakatsu (T.), 2007: 12-15). For the taxonomic rearrangement of land planarians based upon various papers on this animal group, the authors of the ‘Land Planarian Indices Series’ needed new taxonomic names (nomina nova) for several misidentified species reported in old papers. Additionally, the foundation of 5 collective groups for many uncertain land planarian species described only by the external morphology is also needed (cf. -
Platyhelminthes, Tricladida)
Systematics and historical biogeography of the genus Dugesia (Platyhelminthes, Tricladida) Eduard Solà Vázquez ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tdx.cat) i a través del Dipòsit Digital de la UB (diposit.ub.edu) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX ni al Dipòsit Digital de la UB. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX o al Dipòsit Digital de la UB (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tdx.cat) y a través del Repositorio Digital de la UB (diposit.ub.edu) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR o al Repositorio Digital de la UB. -
Downloaded from the Barcode of Life Database (Boldsystems.Org) Public Records
bioRxiv preprint doi: https://doi.org/10.1101/665166; this version posted June 10, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Tracking somatic drift reveals small effective population size of stem cells and high somatic mutation rate in asexual planaria Hosseinali Asgharian1*, Joseph Dunham3, Paul Marjoram2, Sergey V. Nuzhdin3 1 Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco, San Francisco, California, United States of America 2 Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America 3 Program in Molecular and Computational Biology, Dornsife College of Letters, Arts, and Sciences, University of Southern California, Los Angeles, California, United States of America Sergey Nuzhdin Phone: (213) 740-5773 Email: [email protected] bioRxiv preprint doi: https://doi.org/10.1101/665166; this version posted June 10, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Abstract Planarian flatworms have emerged as highly promising models of body regeneration due to the many stem cells scattered through their bodies. Currently, there is no consensus as to the number of stem cells active in each cycle of regeneration or the equality of their relative contributions.