DOCSLIB.ORG

Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Chong, Shasha. 2014. Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting. Doctoral dissertation, Harvard University. Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:12274110 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting A dissertation presented by Shasha Chong to The Department of Chemistry and Chemical Biology in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the subject of Chemistry Harvard University Cambridge, Massachusetts April 2014 ©2014 - Shasha Chong. All rights reserved. Dissertation Advisor: Professor Xiaoliang Sunney Xie Shasha Chong Detection of Single-Molecule Optical Absorption at Room Temperature and Mechanistic Study of Transcriptional Bursting Abstract Advances in optical imaging techniques have allowed quantitative studies of many biological systems. This dissertation elaborates on our efforts in both developing novel imaging modalities based on detection of optical absorption and applying high-sensitivity fluorescence microscopy to the study of biology. Although fluorescence is the most widely used optical contrast mechanism in biological studies because of its background-free detection, many intracellular molecules are intrinsically non-fluorescent and difficult to label without disturbing their natural functions. Therefore, label- free imaging methods based on contrast mechanisms other than fluorescence are highly desirable. In the first part of this dissertation, we explore new methods for sensitive detection of optical absorption. Chapter 2 describes stimulated emission microscopy and two-photon excited photothermal microscopy. We demonstrate label-free imaging of non-fluorescent biological chromophores with high sensitivity and three-dimensional optical sectioning capability. In Chapter 3, we introduce ground-state depletion microscopy and demonstrate room-temperature detection of the absorption signal from a single molecule. This measurement represents the ultimate detection sensitivity of nonlinear optical spectroscopy at room temperature. In the second part of my thesis, I focus on transcription of DNA. Transcription is the first step in gene expression and essential for all cell functions. Recent experiments have shown that iii transcription of highly expressed genes occurs in stochastic bursts. But the origin of such ubiquitous phenomenon was unknown. We investigate the mechanism in bacteria through a series of in vitro and live-cell experiments based on high-sensitivity fluorescence microscopy. Chapter 4 describes a novel high-throughput in vitro single-molecule assay to follow real-time transcription on individual DNA templates. Using this assay, we show in Chapter 5 that positive supercoiling buildup on a DNA segment by transcription slows down transcription elongation and eventually stops transcription initiation. Transcription can be resumed upon gyrase binding to the DNA segment. Furthermore, using single-cell mRNA counting fluorescence in situ hybridization (FISH) assay, we find the extent of transcriptional bursting depends on the intracellular gyrase concentration. Together, these findings prove that transcriptional bursting of highly expressed genes in bacteria is primarily caused by reversible gyrase dissociation from and rebinding to a DNA segment, changing the supercoiling level of the segment. iv Contents Abstract ......................................................................................................................................... iii Table of Contents .......................................................................................................................... v Acknowledgements ....................................................................................................................... x Citation to Previously Published Work ................................................................................... xiii 1 Introduction .............................................................................................................................. 1 1.1 A Technical Introduction ...................................................................................................... 1 1.2 Pump-probe optical microscopy ........................................................................................... 1 1.3 Fluorescence Microscopy ..................................................................................................... 4 1.4 Structure of This Thesis ........................................................................................................ 9 References ................................................................................................................................. 12 2 Label-free imaging of biological chromophores ................................................................... 14 2.1 Background ......................................................................................................................... 15 2.1.1 Biological chromophores with undetectable fluorescence .......................................... 15 2.1.2 Stimulated emission ..................................................................................................... 15 2.1.3 Photothermal microscopy ............................................................................................ 16 2.2 Results and Discussion ....................................................................................................... 16 2.2.1 Principle of stimulated emission microscopy .............................................................. 16 2.2.2 Imaging biological chromophores with stimulated emission microscopy ................... 22 2.2.3 Principle of two-photon excited photothermal microscopy ......................................... 27 2.2.4 Imaging heme proteins with two-photon excited photothermal microscopy ............... 31 2.3 Materials and Methods ........................................................................................................ 35 v 2.3.1 Detailed apparatus of stimulated emission microscopy ............................................... 35 2.3.2 Detailed apparatus of two photon excited photothermal microscopy .......................... 37 2.3.3 Stimulated emission spectrum ..................................................................................... 38 2.3.4 Bacterial genetic constructs ......................................................................................... 39 2.3.5 Sample preparation for mammalian cell imaging ........................................................ 39 2.3.6 Sample preparation for animal tissue imaging ............................................................. 40 References ................................................................................................................................. 41 3 Detection of single-molecule optical absorption at room temperature with ground-state depletion microscopy .................................................................................................................. 44 3.1 Background ......................................................................................................................... 45 3.2 Results and Discussion ....................................................................................................... 47 3.2.1 Principle of ground-state depletion microscopy .......................................................... 47 3.2.2 Imaging gold nanoparticles with ground-state depletion microscopy ......................... 50 3.2.3 Detection of optical absorption from a single molecule at room temperature ............. 52 3.3 Conclusion .......................................................................................................................... 58 3.4 Materials and Methods ........................................................................................................ 59 3.4.1 Detailed apparatus of ground-state depletion microscopy ........................................... 59 3.4.2 Equations for modulation depth of the transmitted probe beam .................................. 60 3.4.3 Estimate of the ground-state depletion signal from a single gold nanoparticle ........... 62 3.4.4 Calculation of the ground-state depletion signal from a single Atto647N molecule ... 67 3.4.5 Calculation of shot noise .............................................................................................. 69 3.4.6 Modulation frequency dependence of ground-state depletion signals ......................... 69 3.4.7 Detectability of single-molecule ground-state depletion signals ................................. 71 vi References ................................................................................................................................. 72 4 A novel single-molecule assay to monitor real-time transcription on individual DNA templates
Load more

Recommended publications
  • The Chinese Chemistry Faculty in Top 30 Schools of United States
    The Chinese Chemistry Faculty in Top 30 Schools of United States The Statistics of the Chinese Chemistry Faculty In Top 30 Schools of United States And some simple statistics: you can review them first. Total Numbers: 43 Top 151: 22 Where did they get their bachelor degrees? Beijing Polytechnic University: Total: 1 Top 15: 1 Dalian University of Technology: Total: 1 Top 15: 1 Fudan University: Total: 2 Top 15: 0 Lanzhou University: Total: 1 Top 15: 0 Nanjing University: Total: 1 Top 15: 1 Nankai University: Total: 2 Top 15: 1 Northwest Telecommunication Engineering Institute: Total: 1 Top 15: 0 Peking University(Beijing University): Total: 11 Top 15: 6 Sichuan University: Total: 1 Top 15: 0 Tianjin Medical College: Total: 1 Top 15: 1 Tsinghua University: Total: 4 Top 15: 3 USTC: Total: 12 Top 15: 5 Wuhan University: Total: 2 Top 15: 0 Zhejiang University(Hangzhou University): Total: 3 Top 15: 3 Do they have master degrees in China? Yes: Total: 10 Top 15: 4 Do they have master degrees outside the Mainland of China? Yes: Total: 6 Top 15: 3 Where did they get their doctor degrees? Top 15 Schools: Total: 24 Top 15: 14 Top 15-30 Schools: Total: 8 Top 15: 4 Other Schools in United States: Total: 9 Top 15: 4 Schools outside United States: Total: 2 Top 15: 0 When did they get the bachelor degrees? (lacking some data) 80th: Total: 21 Top 15: 10 90th: Total: 20 Top 15: 11 Have they already got the tenures? Yes: Total: 27 Top 15: 15 Details MIT: Jianshu Cao Associate Professor of Chemistry B.
    [Show full text]
  • Gene Regulation and Chromatin Structure of Mammalian Olfactory Receptors
    Gene Regulation and Chromatin Structure of Mammalian Olfactory Receptors The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Tan, Longzhi. 2018. Gene Regulation and Chromatin Structure of Mammalian Olfactory Receptors. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences. Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:41129184 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA Gene regulation and chromatin structure of mammalian olfactory receptors A dissertation presented by Longzhi Tan to The Committee on Higher Degrees in Systems Biology in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the subject of Systems Biology Harvard University Cambridge, Massachusetts April 2018 © 2018 Longzhi Tan All rights reserved. Dissertation Advisor: Professor Xiaoliang Sunney Xie Longzhi Tan Gene regulation and chromatin structure of mammalian olfactory receptors Abstract Mammals sense odors by expressing the gene family of olfactory receptors (ORs). Despite the massive family size — around 1,000 OR genes in the mouse genome and 400 in human, each sensory neuron randomly expresses one, and only one, OR. This phenomenon, termed the “one- neuron-one-receptor” rule, underlies both odor sensing in the nose and the formation of an odor map in the brain. However, it remains a mystery how this rule is established. Combining theoretical modeling, single-cell transcriptomics, spatial transcriptomics, and single-cell 3D genome structures, we investigated the regulation of OR genes during neuronal development.
    [Show full text]
  • The Grand Challenges in the Chemical Sciences
    The Israel Academy of Sciences and Humanities Celebrating the 70 th birthday of the State of Israel conference on THE GRAND CHALLENGES IN THE CHEMICAL SCIENCES Jerusalem, June 3-7 2018 Biographies and Abstracts The Israel Academy of Sciences and Humanities Celebrating the 70 th birthday of the State of Israel conference on THE GRAND CHALLENGES IN THE CHEMICAL SCIENCES Participants: Jacob Klein Dan Shechtman Dorit Aharonov Roger Kornberg Yaron Silberberg Takuzo Aida Ferenc Krausz Gabor A. Somorjai Yitzhak Apeloig Leeor Kronik Amiel Sternberg Frances Arnold Richard A. Lerner Sir Fraser Stoddart Ruth Arnon Raphael D. Levine Albert Stolow Avinoam Ben-Shaul Rudolph A. Marcus Zehev Tadmor Paul Brumer Todd Martínez Reshef Tenne Wah Chiu Raphael Mechoulam Mark H. Thiemens Nili Cohen David Milstein Naftali Tishby Nir Davidson Shaul Mukamel Knut Wolf Urban Ronnie Ellenblum Edvardas Narevicius Arieh Warshel Greg Engel Nathan Nelson Ira A. Weinstock Makoto Fujita Hagai Netzer Paul Weiss Oleg Gang Abraham Nitzan Shimon Weiss Leticia González Geraldine L. Richmond George M. Whitesides Hardy Gross William Schopf Itamar Willner David Harel Helmut Schwarz Xiaoliang Sunney Xie Jim Heath Mordechai (Moti) Segev Omar M. Yaghi Joshua Jortner Michael Sela Ada Yonath Biographies and Abstracts (Arranged in alphabetic order) The Grand Challenges in the Chemical Sciences Dorit Aharonov The Hebrew University of Jerusalem Quantum Physics through the Computational Lens While the jury is still out as to when and where the impressive experimental progress on quantum gates and qubits will indeed lead one day to a full scale quantum computing machine, a new and not-less exciting development had been taking place over the past decade.
    [Show full text]
  • CV Brian English 2017 09 26
    HHMI/Janelia iiiiiiiiiii iiiiiiiiiii iiiiiiiiiii 19700 Helix Drive Ashburn, VA 20147 BRIAN PATRICK ENGLISH iiiiiiiiiii iiiiiiiiiii iiiiiiiiiii [email protected] http://www.brianpenglish.com EDUCATION Single Molecule Studies of Enzymatic Dynamic Fluctuations PhD Harvard University 11/2007 Advisor: Xiaoliang Sunney Xie MA Harvard University 11/2003 Chemistry and Chemical Biology BA Cornell University 01/2001 Bachelor of Arts with Distinction in all Fields PROFESSIONAL EXPERIENCE 01/2013– Present Howard Hughes Medical Institute Senior Scientist Research Scientist (01/2015 – 12/2015) Janelia Research Campus Ashburn VA Research Specialist (01/2013 – 12/2014) Albert Einstein College of Medicine Postdoctoral Fellow 09/2010 – 12/2012 Bronx NY Anatomy and Structural Biology Uppsala University Postdoctoral Fellow 09/2007 – 08/2010 Uppsala Sweden Cell and Molecular Biology Harvard University Graduate Research Fellow 09/2001 – 08/2007 Cambridge MA Chemistry and Chemical Biology Cornell University Research Technician 01/2001 – 08/2001 Ithaca NY Laboratory of Harold A. Scheraga Cornell University Undergraduate Research Fellow 09/1997 – 12/2000 Ithaca NY Chemistry and Chemical Biology HONORS 2015 AAAS Newcomb Cleveland Prize (Lattice light-sheet microscopy) 02/2016 Postdoctoral Representative to the Einstein Senate 10/2010 – 12/2012 Young Researcher Participant of the 59th Meeting of Nobel Laureates in Lindau 06/2009 Student-nominated Fieser Speaker Harvard Chemistry and Chemical Biology 04/2007 Eli Lilly Poster Presentation Award 19th Annual
    [Show full text]
  • Harvard Chinese Life Science Symposium Harvard Chinese Life Science
    2015 Harvard Chinese Life Science Symposium Harvard Chinese Life Science Annual Research Symposium March 28, 2015 Jimmy Fund Auditorium, Harvard Medical School, 35 Binney Street, Boston, MA, 02115 Harvard Medical School Chinese Scholars & Scientists Association Harvard School of Public Health Chinese Students & Scholars Association 1 2015 Harvard Chinese Life Science Symposium Organizing Committee Advisors Xi He, Professor, Boston Children’s Hospital, Harvard Medical School Xiaole Shirley Liu, Professor, Dana-Farber Cancer Institute, Harvard School of Public Health Yi Zhang, Professor, Boston Children’s Hospital, Harvard Medical School Jiping Wang, Assistant Professor, Brigham and Women’s Hospital, Harvard Medical School William Pu, Professor, Boston Children’s Hospital, Harvard Medical School Jean Zhao, Associate Professor, Dana-Farber Cancer Institute, Harvard Medical School Jing Ma, Associate Professor, Brigham and Women’s Hospital, Harvard School of Public Health Frank Hu, Professor, Harvard School of Public Health Jianzhu Chen, Professor, Massachusetts Institute of Technology Organizing Committee Harvard Medical School - Chinese Scientists and Scholars Association (HMS-CSSA) Jin Zhang, Shaokun Shu, Zhe Ji, Qing Li, Shaojun Tang, Zhiqiang Lin, Dongpo Cai, Xiaofeng Wang, Wei Li, Tengfei Xiao, Chunxiao Yu, Xingxing Kong, Min Tan, Chunyao Wei, Wen Chen, Jiaren Liu, Ji Li, Hao Huang, Jun Huang, Hongguang Xia, Xiaoyang Zhang, Wenqing Cai, Xuezhe Han, Bin Li, Yu Qian, Dapeng Yan, Yiying Zhu, Song Yang; Sui Wang Harvard School of Public Health - Chinese Students and Scholars Association (HSPH-CSSA) Zhaozhong Zhu, Qian Di, Yinyin Xu, Xihao Li, Xiaoyu Li, Haiyue Zhang 2 2015 Harvard Chinese Life Science Symposium Agenda 9:00am – 9:30am Registration and Light Refreshment Opening Remarks Dr.
    [Show full text]
  • 2019单细胞组学国际研讨会 Single Cell Omics Beijing 2019 Symposium
    2019单细胞组学国际研讨会 Single Cell Omics Beijing 2019 Symposium Scan this QR code to learn more about us. 北京未来基因诊断 北京大学生物医学 高精尖创新中心（ICG） 前沿创新中心（BIOPIC） Host Organization Contents Committee Host Organization 01 Co-Chair Xiaoliang Sunney Xie Lee Shau-kee Professor of Peking University Director, Biomedical Pioneering Innovation Center Director, Beijing Advanced Innovation Center for Genomics Committee 01 Member, US National Academy of Sciences Member, US National Academy of Medicine Member, American Academy of Arts and Sciences Foreign Member, Chinese Academy of Sciences Program 02 Co-Chair Fuchou Tang Associate Director of Beijing Advanced Innovation Center for Genomics (ICG) Principle Investigator of Biomedical Pioneering Innovation Center (BIOPIC) Professor of School of Life Sciences, Peking University Investigator of Center of Life Sciences (CLS) Speakers 06 Winner of National Science Fund for Distinguished Young Scholars Co-Chair Yanyi Huang Associate Director of Beijing Advanced Innovation Center for Genomics (ICG) Principle Investigator of Biomedical Pioneering Innovation Center (BIOPIC) Professor of College of Engineering, Peking University Investigator of Center of Life Sciences (CLS) Winner of National Science Fund for Distinguished Young Scholars 01 Program 2019单细胞组学国际研讨会 Single Cell Omics Beijing 2019 Symposium Program Understanding pancreatic organogenesis 13:30-14:00 Cheng-Ran Xu at single-cell level 14:00-14:30 A Human Cell Landscape for Cell Type Identification at the Single-Cell Level Guoji Guo Session 3 Genomic Variations Chair:
    [Show full text]
  • Xiaoliang Sunney Xie - Wikipedia
    Xiaoliang Sunney Xie - Wikipedia https://en.wikipedia.org/wiki/Xiaoliang_Sunney_Xie Xiaoliang Sunney Xie Xiaoliang Sunney Xie (Chinese: 谢晓�; born 1962 in Beijing, China) is Xiaoliang Sunney Xie an American biochemist, considered a founding father of single-molecule biophysical chemistry and single-molecule enzymology$[2] Contents Early life Research Honors and awards Selected Literature Single-Cell Genomics Gene Expression and Regulation Single Molecule Enzymology Coherent Raman Imaging Born June 24, 1962 Single Molecule Imaging Beijing, China References Education BSc in External links Chemistry from Peking University Early life PhD in Physical Chemistry from 'e received his B.Sc$ in Chemistr" from Peking ,niversit" in 1984, and UC San Diego his Ph.D$ in Physical Chemistry in 1990 from ,niversit" of California at Known for Single Molecule San Diego$ After a brief postdoctoral appointment at ,niversit" of Enzymology, Chicago, he joined Pacific Northwest National Laboratory, 2here he rose Coherent from Senior 4esearch Scientist to Chief Scientist$ In 1998, he became the Raman first tenured professor at 'arvard ,niversity among Chinese Scholars 2ho Imaging, came to the United States since Chinese economic reform$ Single Cell Genomics Research Awards Albany Prize in 'e had been the Mallinckrodt *rofessor of Chemistry and Chemical Medicine and Biology at 'arvard University until 2018, when he became the Lee Shau- Biomedical kee *rofessor of Peking ,ni(ersity. Currently, he is also the Director of Research 2015, Biomedical Pioneering 5nnovation Center (BIOP5C), and the /irector of Peter Debye Beijing Advanced Innovation Center for Genomics (ICG), both at Peking Award in 1 of 7 11/26/19, 2:15 AM Xiaoliang Sunney Xie - Wikipedia https://en.wikipedia.org/wiki/Xiaoliang_Sunney_Xie ,niversit"$ Physical Chemistry As a pioneer of single-molecule biophysical chemistry, coherent Raman 2015, scattering microscopy, and single-cell genomics, he made major Ellis R.
    [Show full text]
  • 2013 NIH Common Fund Single Cell Analysis Investigators Meeting
    2013 NIH Common Fund Single Cell Analysis Investigators Meeting Where: Residence Inn, Bethesda, Maryland When: April 15-16, 2013 The NIH Common Fund held a Single Cell Analysis Investigators Meeting on April 15-16, 2013 in Bethesda, Maryland. This year’s meeting objectives are to: • Disseminate current research findings in single cell analysis. • Convene the funded SCAP investigative teams to update the community on their research progress, problems, and new opportunities in single cell analysis. • Identify roadblocks and emerging challenges in the understanding of single cell level heterogeneity. • Consider current conceptual, technical, and/or methodological challenges in single cell analysis and brainstorm possible solutions. • Discuss major themes in basic and/or clinical research for which additional focus on single cell analysis may provide the most significant and broadest impact. • Determine major biomedical research opportunities that can be addressed by single cell analysis. • Discuss how relevant groundbreaking technologies and approaches in SCA can be disseminated to the research community. • Facilitate new research collaborations between funded grantees and others interested in entering the Single Cell Analysis field. • Build synergy between the Single Cell Analysis Program and other projects funded by NIH. The meeting incorporated presentations by 5 invited keynote speakers and 15 funded investigators, 28 poster presentations, and 13 lightning talks, and yielded opportunities to review progress, collaborate, and brainstorm novel ideas. Over 160 participants attended this year’s meeting, with representatives in academia (55%), government (30%), industry (10%), and nonprofits (5%). Almost one-third of attendees were funded investigators and collaborators, while 21% were postdoctoral fellows and graduate students. Five scientific sessions were held.
    [Show full text]
  • “Breakthroughs in Ultraspectroscopy & Ultramicroscopy”
    the university of texas at austin spring 1999 CHEMICALCompositionschemistry & biochemistry departmental newsletter “Breakthroughs in Ultraspectroscopy & Ultramicroscopy” Conference celebrates opening of laboratory for spectroscopic imaging (LSI) On April 23, in the Flawn Academic Center, a scientific conference was held in recognition of the opening of the Laboratory for Spectroscopic Imaging LSI (http://www.cm.utexas.edu/~barbara/) in the Department of Chemistry and Biochemistry. The conference featured talks by leading researchers in the area of spectroscopy, photochemistry and/or microscopy including: Marye Anne Fox (North Carolina University – Raleigh), Mostafa El-Sayed (Georgia Institute of Technology), Xiaoliang Sunney Xie (Environmental Molecular Sciences Laboratory and Harvard University) and Frans De Schryver (KULeuven, Belgium). The LSI is a state-of-the-art research facility for laser spectroscopy. The focus of LSI is the study of basic physical and biophysical chemical problems with ultrafast spectroscopy and ultra-microscopy. The LSI is both a user facility and the major research laboratories of the research group of Paul (standing l-r) Xiaoliang Sunny Xie, Hiroshi Masuhara, Frans De Schryver, Barbara, who joined our faculty in the fall. The laboratory contains state-of- Don O’Connor (seated l-r) Larry Faulkner, Mary Anne Fox, the-art research instrumentation including femtosecond and picosecond Mostafa El-Sayed, Paul Barbara spectrometers, various scanning probe microscopy/spectroscopy apparatuses (NSOM,AFM,STM), and two single molecule spectroscopy instruments. One main thrust in the LSI is the use of femtosecond spectroscopy to investigate intermolecular and intramolecular atomic motions during ultrafast chemical processes in solution. Some of the processes under investigation include the relaxation dynamics of the hydrated electron, intermolecular electron transfer in metal-metal-mixed valence complexes, and the conformational structure and dynamics of RNA-protein complexes.
    [Show full text]
  • University of Rochester 2012-2013
    University of Rochester 2012-2013 CONTACT ADDRESS Table of Contents University of Rochester Department of Chemistry 3 Chemistry Department Faculty and Staff 404 Hutchison Hall RC Box 270216 4 Letter from the Chair - Robert Boeckman, Jr. Rochester, NY 14627-0216 6 Meet the New Chair - Todd D. Krauss PHONE (585) 275-4231 7 Todd D. Krauss Elected APS Fellow EMAIL 8 Donors to the Chemistry Department [email protected] 10 Alumni News WEBSITE http://www.chem.rochester.edu 13 Richard Eisenberg Receives William H. Nichols and Fred Basolo Medals CREDITS 14 Sloan Research Fellow Dan Weix EDITOR Thieme Chemistry Journal & Pfizer Green Chemistry Awards Terri Clark 15 Harrison Howe : Sunney Xie LAYOUT & DESIGN EDITOR Nanomaterials Symposium 2013 John Bertola (B.A. ’09, M.S. ’10W) 16 Terri Clark 17 The 2013 Chemistry-Biology-Biophysics Cluster Retreat REVIEWING EDITORS 18 Two Teaching Awards - Frontier & Hafensteiner Lynda McGarry Terrell Samoriski 19 ACS on Campus at Carlson Library Barbara Snaith 20 Student Awards and Accolades COVER ART AND LOGOS Breanna Eng (‘13) 23 Faculty News Sheridan Vincent 33 Chemistry Welcomes Ignacio Franco WRITING CONTRIBUTIONS 54 Faculty Publications Department Faculty Breanna Eng (‘13) 59 Postdoctoral Fellows Terri Clark Select Alumni 60 Commencement 2013 PHOTOGRAPHS 62 Student Awards UR Communications John Bertola (B.A. ‘09, M.S. ‘10W) 64 Seminars and Colloquia Ria Casartelli Sheridan Vincent 69 Staff News Thomas Krugh Breanna Eng (‘13) 74 Instrumentation Terri Clark UR Friday Photos from 75 Departmental Funds various contributors 76 Alumni Update Form 1 2 Faculty and Staff FACULTY PROFESSORS OF CHEMISTRY STAFF Robert K. Boeckman, Jr.
    [Show full text]
  • Major Topics
    COLD SPRING HARBOR ASIA ORGANIZERS(Speaker, Affilia�on, Country/Region） John Marioni Frontiers in European Molecular Biology Laboratory, UK Nicholas Navin The University of Texas MD Anderson Cancer Center, USA Orit Rozenblatt-Rosen Single Cell Broad Institute, USA Fuchou Tang Genomics Peking University, China Suzhou, China November 5-9, 2018 MAJOR TOPICS Abstract deadline: September 14, 2018 Technology development Developmental Biology Cancer Biology Computational biology Single cell epigenomics Single cell transcriptional profiling Single cell DNA analysis Single cell imaging Next-generation sequencing KEYNOTE SPEAKERS(Speaker, Affilia�on, Country/Region） Stephen Quake, Stanford University, USA Xiaoliang Sunney Xie, Peking Universtiy, CHINA INVITED SPEAKERS(Speaker, Affilia�on, Country/Region） Andrew Adey, Oregon Health & Sciences University, USA Alicia Oshlack, Murdoch Children's Research Institute, AUSTRALIA Fan Bai, Peking University, CHINA Woong-Yang Park, Samsung Medical Center, SOUTH KOREA Long Cai, California Institute of Technology, USA Shyam Prabhakar, Genome Institute of Singapore, SINGAPORE Charles Gawad, St. Jude Children's Research Hospital, USA Orit Rozenblatt-Rosen, Broad Institute, USA Guoji Guo, Zhejiang University School of Medicine, CHINA Rahul Satija, New York Genome Center, USA Yanyi Huang, Peking Universtiy, CHINA Fuchou Tang, Peking University, CHINA Gavin Kelsey, The Babraham Institute, UNITED KINGDOM Thierry Voet, Wellcome Sanger Institute, UK Devon Lawson, University of California, Irvine, USA Fiona Watt, King’s College London, UNITED KINGDOM Hae-Ock Lee, Samsung Medical Center, SOUTH KOREA Nir Yosef, UC Berkeley, USA Cold Spring Harbor Asia (CSHA) is the John Marioni, European Molecular Biology Laboratory, UNITED KINGDOM Kun Zhang, University of California, San Diego, USA , Peking University, CHINA Asia-Pacific subsidiary of New York Nicholas Navin, The University of Texas MD Anderson Cancer Center, USA Zemin Zhang based Cold Spring Harbor Laboratory (CSHL).
    [Show full text]
  • Download the Program Book for OCG 2018
    OGC 2018 The Optoelectronics Global Conference Shenzhen Convention & Exhibition Center, Shenzhen, China 4-7 September, 2018 2018 The Optoelectronics Global Conference (OGC 2018) Sponsored by Organized by Patrons 2 2018 The Optoelectronics Global Conference (OGC 2018) Contents Page About OGC 2018…………………………………………. 4 Presentation Instructions………………………………….. 6 Conference Committee……………………………………. 7 Plenary Speakers…………………………………………... 10 Technical Program………………………………………… 13 th Day 1 (Sep 5 ) ……………………………………….…. 19 th Day 2 (Sep 6 )……………………………………….... 42 th Day 3 (Sep 7 )………………………………………....... 88 Conference Venue...…………………………………………. 108 Travel Information...………………………………………… 113 Note……………………………………………………….. 116 3 2018 The Optoelectronics Global Conference (OGC 2018) About OGC 2018 The big leaps in optoelectronic technology and academia have drawn increasing attention from the industry community which is always in searching of innovative solutions. The Optoelectronics Global Conference (OGC) was created to pave the way connecting optoelectronic academia and industry as well as connecting China and the rest of the world. 2018 the 3rd Optoelectronics Global Conference will be held concurrently with the 20th China International Optoelectronic Exposition (CIOE) in Shenzhen. The conference aims to promote interaction and exchange of various disciplines among professionals in academia and industry at home and abroad. In addition, it also serves to turn technologies into industrial applications. It’s expected that 300-500 professionals will attend the conference. OGC will be an ideal platform for scholars, researchers and professionals to exchange insights and discuss the development of optoelectronics industry. It will be a perfect gathering to learn about new perspectives, technologies and trends which might pushes the boundaries of the technology and eventually creates a broader future for optoelectronics applications. 8 symposia will be talked on the conference with the topics covering precision optics, optical communications, lasers, infrared applications, and fiber sensors.
    [Show full text]