UTILISATION of MATURED GREEN BANANA (Musa Paradisiaca Var
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UTILISATION OF MATURED GREEN BANANA (Musa Paradisiaca var. Awak) FLOUR AND OAT BETA GLUCAN AS FIBRE INGREDIENTS IN NOODLES CHONG LI CHOO UNIVERSITI SAINS MALAYSIA 2007 UTILISATION OF MATURED GREEN BANANA (Musa Paradisiaca var. Awak) FLOUR AND OAT BETA GLUCAN AS FIBRE INGREDIENTS IN NOODLES by CHONG LI CHOO Thesis submitted in fulfillment of the requirements for the degree of Master of Science July 2007 i ACKNOWLEDGEMENTS This thesis would not have been accomplished without the wise and careful guidance of my supervisors, and the kind help from my family and friends. I am greatly indebted to Associate Professor Dr Noor Aziah Abdul Aziz after her persistent effort in guiding me and supervised me in doing my master research efficiently and productively. All that I have learnt through Associate Professor Dr Noor Aziah Abdul Aziz will be greatly beneficial for my future career. My gratefulness and thanks is directed to all the lectures, friends and staffs of the Food Division School of Technology for their kindly help and support during my experimental work. I would like to extend special thanks to Thuan Chew, Rosnani and Mr. Amaizan for their gracious help during the course of this study. I want to express my gratitude to Ministry of Science, Technology, and Innovation (MOSTI) Malaysia for the National Science Fellowship (NSF) scholarship, which enable me to complete this research without financial difficulties. I am also indebted to Associate Professor Dr. Shaida Fariza Sulaiman and her students from School of Biological Science, USM for their sincere support and assistant during the experimental work of antioxidant properties in my research. My sincere gratitude goes to my family for standing by me regardless of what happened, supporting me, believing in me and encouraging me throughout these years. Li Choo April 2007 ii TABLE OF CONTENTS Page i TITLE ii ACKNOWLEDGEMENTS TABLE OF CONTENTS iii LIST OF TABLES v LIST OF FIGURES vi LIST OF APPENDICES vii LIST OF PUBLICATIONS & SEMINARS vii ABSTRAK ix ABSTRACT xi CHAPTER 1 : INTRODUCTION 1 CHAPTER 2 : LITERATURE REVIEW 6 2.1 Musa 6 2.1.1 Plantains 7 2.1.2 Nutritional values of Musa 8 2.1.3 Banana Flour 9 2.2 WHEAT FLOUR NOODLE 11 2.2.1 Alkaline noodles 12 2.2.2 General noodle processing technology 14 2.2.3 Assessing noodle quality 15 2.2.3.1 Colour 16 2.2.3.2 Texture 17 2.2.3.3 Cooking characteristic 18 2.2.4 Dried noodle 18 2.3 DIETARY FIBRE 19 2.3.1 Hemicellulose 21 2.4 RESISTANT STARCH (RS) 21 2.5 OAT BETA (β) GLUCAN 23 2.6 ANTIOXIDANT 24 2.7 GLYCEMIC INDEX 25 2.8 FUNCTIONAL FOOD / STRATEGIES TO INCREASE 27 NUTRITIONAL VALUE IN FOOD PRODUCTS 2.9 COMPOSITE FLOUR 29 2.10 NATIONAL FORTIFICATION PRACTICES OF NOODLE 29 iii CHAPTER 3 : MATERIALS AND METHODS 32 3.1 OUTLINE OF STUDY 32 3.2 MATERIALS 34 3.3 GREEN BANANA FLOUR (Musa paradisiacal var. Awak) 34 PREPARATION 3.4 NOODLES FORMULATION AND PREPARATION 34 3.5 PROXIMATE ANALYSIS 36 3.5.1 Determination of moisture content 36 3.5.2 Determination of crude protein 36 3.5.3 Determination of crude fibre 37 3.5.4 Determination of crude fat 38 3.5.5 Determination of ash 38 3.6 DETERMINATION OF ESSENTIAL MINERAL CONTENT 39 3.7 TOTAL DIETARY FIBRE ANALYSIS 40 3.8 DETERMINATION OF RESISTANT STARCH (RS) 41 3.9 DETERMINATION OF TOTAL STARCH 43 3.10 DETERMINATION OF AMYLOSE CONTENT 43 3.11 DETERMINATION OF AMYLOPECTIN CONTENT 44 3.12 PHYSICAL PROPERTIES 44 3.12.1 Pasting properties 44 3.12.2 Cooking characteristic 45 3.12.3 Texture profile analysis (TPA) 46 3.12.4 Colour measurement 46 3.13 IN-VITRO CARBOHYDRATE DIGESTIBILITY 47 3.14 GLYCEMIC INDEX (GI) 48 3.15 ANTIOXIDANT PROPERTIES 50 3.15.1 Preparation of noodle extracts 50 3.15.2 Determination of total phenolic compounds 50 3.15.3 Scavenging effect on DPPH radical 50 3.15.4 Determination of antioxidant activity 51 3.16 SCANNING ELECTRON MICROSCOPE (SEM) 51 3.17 FLOUR SWELLING PROPERTIES 52 3.18 STORAGE EVALUATION 52 3.19 SENSORY EVALUATION 53 3.20 DRIED NOODLE PREPERATION 54 iv 3.20.1 Fracturability of dried noodle 55 3.21 STATISTICAL ANALYSIS 55 CHAPTER 4: RESULTS AND DISCUSSION 56 4.1 PRELIMINARY STUDY 56 4.2 PROXIMATE ANALYSIS OF NOODLES 58 4.3 TOTAL DIETARY FIBRE AND RESISTANT STARCH 60 4.4 ESSENTIAL MINERALS 64 4.5 AMYLOSE, AMYLOPETIN AND TOTAL STARCH 67 4.6 PASTING PROPERTIES 69 4.7 COOKING QUALITY 71 4.8 TEXTURE PROFILE ANALYSIS 73 4.9 COLOUR MEASUREMENT 75 4.10 IN-VITRO CARBOHYDRATE DIGESTIBILITY 77 4.11 GLYCEMIC INDEX 79 4.12 ANTIOXIDANT PROPERTIES 82 4.13 SWELLING PROPERTIES AND SOLUBILITY 85 4.14 SCANNING ELECTRON MICROSCOPE (SEM) 87 4.15 STORAGE EVALUATION – MICROBIAL COUNT 92 4.16 SENSORY EVALUATION 95 4.17 RESISTANCE OF DRY NOODLES TO FRACTURE 97 CHAPTER 5 : CONCLUSIONS 99 CHAPTER 6 : RECOMMENDATIONs FOR FUTURE RESEARCH 101 REFERENCES 102 APPENDICES LIST OF PUBLICATION AND SEMINARS v LIST OF TABLES Table 2.1 World production statistics of plantainin 2003 (FAO, 2003) 7 Table 2.2 Nutritional values for the plantain and banana (per 100g edible 10 portion) (Morton, 1997) Table 2.3 Noodle formulation and processing in the laboratory (Hou and 13 Kruk, 1998) Table 2.4 Asian noodle texture evaluation (Hou and Kruk, 1998) 18 Table 2.5 Total phenolic content in different type of fruits, cereals and 25 vegetables. Table 2.6 Food with different Glycemic Index (GI) (Agatston, 1995) 26 Table 2.7 Selected functional food in market. 28 Table 3.1 Formulations of different type of noodles. 34 Table 4.1 Proximate composition of banana flour and wheat flour 56 Table 4.2 Pasting properties of banana flour and wheat flour 57 Table 4.3 The proximate composition of pre-cooked noodles. 58 Table 4.4 Resistant starch (RS) content in different types of pre-cooked 63 noodles. Table 4.5 Different essential mineral contents in pre-cooked noodles. 66 Table 4.6 Amylose, amylopectin and total starch content in pre-cooked 67 noodles. Table 4.7 Peak viscosity, breakdown and final viscosity of different 69 mixture of flour meal. Table 4.8 Optimum cooking time, cooked weight and cooking loss of 71 pre-cooked noodles. Table 4.9 Tensile strength, firmness and stickiness of different types of 73 noodles. Table4.10 In-vitro carbohydrate digestibility of different types of pre- 77 cooked noodle. Table4.11 Glycemic index of four different types of pre-cooked noodles. 79 Table4.12 Antioxidant activity and total phenolic content of pre-cooked 82 noodles. Table4.13 Swelling properties, swelling properties and solubility of 85 different types of flour mixture. Table4.14 Breaking stress of raw and pre-cooked noodles dried in 97 different drying temperature cycles. vi LIST OF FIGURES Figure 2.1 Noodle making process (Hou and Kruk, 1998) 14 Figure 2.2 Structure of flavonoid pigment (tricine) from wheat flour 17 (Hudsan et al., 2000) Figure 2.3 Chemical structure of the main components of hemicellulose 21 (Resende, 2005) Figure 3.1 Flowchart of Overall Analyses 33 Figure 3.2 Flowchart of Phase II Analyses 35 Figure 3.3 Flowchart of Phase III Analyses 47 Figure 3.4 Flowchart of Phase IV Analyses 54 Figure 4.1 Comparison of IDF, SDF and TDF content of pre-cooked 60 noodle without oat β-glucan. Figure 4.2 Comparison of IDF, SDF and TDF content of pre-cooked 61 noodle with oat β-glucan. Figure 4.3 Comparison of TDF content between pre-cooked noodle 62 without and with oat β-glucan Figure 4.4 Colour measurement for raw noodles 75 Figure 4.5 Colour measurement for pre-cooked noodles 76 Figure 4.6 In-vitro starch hydrolysis rate of four different types of pre- 81 cooked noodles. Figure 4.7 Microstructure of cross section of raw control noodle (without 88 oat β-glucan) (magnification = 500x) Figure 4.8 Microstructure of cross section of raw control noodle (with oat 88 β-glucan) (magnification = 500x) Figure 4.9 Microstructure of cross section of raw 30% BF-incorporated 89 noodle (without oat β-glucan) (magnification = 500x) Figure 4.10 Microstructure of cross section of raw 30% BF-incorporated 89 noodle (with oat β-glucan) (magnification = 500x) Figure 4.11 Microstructure of cross section of pre-cooked control noodle 90 (without oat β-glucan) (magnification = 250x) Figure 4.12 Microstructure of cross section of pre-cooked control noodle 91 (with oat β-glucan) (magnification = 250x) Figure 4.13 Microstructure of cross section of pre-cooked 30% BF- 91 incorporated noodle (without oat β-glucan) (magnification = 500x) vii Figure 4.14 Microstructure of cross section of pre-cooked 30% BF- 92 incorporated noodle (with oat β-glucan) (magnification = 500x) Figure 4.15 Aerobic plat count for different types of pre-cooked noodles 93 during storage at 4oC Figure 4.16 Mold and yeast count for different types of pre-cooked 94 noodles during storage at 4oC Figure 4.17 Result of sensory analysis for control and 30%BF- 95 incorporated pre-cooked noodle (with and without oat β- glucan) LIST OF APPENDICES Appendix A The Control and 30% BF-incorporated Noodle (without and with added oat β-glucan) Appendix B Sensory Evaluation Scorecard for Noodle LIST OF PUBLICATIONS & SEMINARS 1.1 Effect of green banana flour (Musa paradisiaca) and oat beta-glucan in yellow alkaline noodle on nutritional properties and sensory evaluation. Paper submitted for 10th ASEAN Food Conference, August 2007, Kuala Lumpur, Malaysia.