foods Article Study on the Sleep-Improvement Effects of Hemerocallis citrina Baroni in Drosophila melanogaster and Targeted Screening to Identify Its Active Components and Mechanism Yuxuan Liang, Riming Huang, Yongchun Chen, Jing Zhong, Jie Deng, Ziyi Wang, Zhuojun Wu, Meiying Li, Hong Wang and Yuanming Sun * College of Food Science, South China Agricultural University, Guangzhou 510642, China;
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[email protected] Abstract: Hemerocallis citrina Baroni (HC) is an edible plant in Asia, and it has been traditionally used for sleep-improvement. However, the bioactive components and mechanism of HC in sleep- improvement are still unclear. In this study, the sleep-improvement effect of HC hydroalcoholic extract was investigated based on a caffeine-induced insomnia model in Drosophila melanogaster (D. melanogaster), and the ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-ESI-Orbitrap-MS) and network pharmacology strategy were further combined to screen systematically the active con- Citation: Liang, Y.; Huang, R.; Chen, stituents and mechanism of HC in sleep-improvement. The results suggested HC effectively regu- Y.; Zhong, J.; Deng, J.; Wang, Z.; Wu, lated the number of nighttime activities and total sleep time of D. melanogaster in a dose-dependent Z.; Li, M.; Wang, H.; Sun, Y.