Le Cong, Ph.D.

Broad Institute, Room 6029, 415 Main Street, Cambridge, MA 02142 E-mail: [email protected] (work), [email protected] (personal) Office: +(1) 617-714-7879, Mobile: (+1) 617-823-6132 EDUCATION l , 09/2009~09/2013 Ph.D. in Biological and Biomedical Sciences (September, 2013) Certificate in Leder Human Biology and Translational Medicine Dissertation: Engineering Technology and Its Application in Mammalian Cells l School of Life Sciences, Tsinghua University 08/2006~07/2009 B.S., with highest honor (June, 2009) • Vice President & Director of R&D Center, Tsinghua Student Association of Science and Technology • Overall & Science GPA: 3.94/4.0 & 4.0/4.0 (converted to 4.0 scale) l Department of Electronic Engineering, Tsinghua University 08/2005~08/2006

RESEARCH EXPERIENCE AND EMPLOYMENT HISTORY l Post-doctoral Associate (CRI Irvington Fellow). Department of Biology, Institute of Technology. Klarman Cell Observatory, Broad Institute of MIT and Harvard. 12/2014~Present – Research advisor: Dr. – Design and development of high-throughput single-cell technology and analytical methods – Establish genetic and epigenetic tools for immune cells based on CRISPR-Cas9 system – Apply genetic perturbation, single-cell analysis, and systems biology approaches to understand and develop novel therapeutics for cancer immunotherapy l Graduate Student (until 09/2013) and Post-doctoral Associate. The Broad Institute of MIT and Harvard. McGovern Institute for Brain Research, MIT. Harvard Medical School. 01/2011~12/2014 – Research advisor: Dr. – Design and development of efficient, open-source, multiplex genome engineering technologies – Develop and improve TAL Effector (TALE/TALEN) tools for mammalian genome targeting – Characterize and engineer CRISPR-Cas9 systems to enable RNA-guided gene editing – Apply genome engineering in human disease modeling and in vivo therapeutics l Graduate Student. Department of Genetics, Harvard Medical School. 08/2010~12/2010 – Research advisor: Dr. George Church and Dr. Feng Zhang – Improve and optimize Multiplex Automated Genomic Engineering (MAGE) technology – Design and engineer TAL Effector system for modulation of mammalian transcription l Trainee. McKinsey & Company. (Offer for Business Analyst gratefully declined) 05/2008~09/2008 l Undergraduate. Tsinghua-Nankai-IBP Joint Group for Structural Biology. 07/2007~07/2009 – Advisor: Dr. Zihe Rao, MCAS – Elucidate viral replication mechanism through X-ray crystallography and biochemical approaches – Structural-based drug design for Severe Acute Respiratory Syndrome (SARS) PUBLICATIONS 1. Tabebordbar M*, Zhu K*, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM8, Wagers AJ. In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Science. 2016. (* Equal contribution) 2. Nishimasu H, Cong L, Yan WX, Ran FA, Zetsche B, Li Y, Kurabayashi A, Ishitani R, Zhang F, Nureki O. Crystal structure of Staphylococcus aureus Cas9. Cell. 2015. 3. Xu H, Xiao T, Chen CH, Li W, Meyer CA, Wu Q, Wu D, Cong L, Zhang F, Liu JS, Brown M, Liu XS. Sequence determinants of improved CRISPR sgRNA design. Genome Research. 2015. 4. Ran F*, Cong L*, Yan W*, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015. (* Equal contribution) 5. Li W, Xu H, Xiao T, Cong L, Love MI, Zhang F, Irizarry RA, Liu JS, Brown M, Liu XS. MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens. Genome Biology. 2014. 6. Cong L#, Zhang F. Genome Engineering Using CRISPR-Cas9 System. Methods in Molecular Biology. Chromosomal Mutagenesis. 2014. (# Corresponding author) 7. Chen S, Xue Y, Wu X, Cong L, Bhutkar A, Bell EL, Zhang F, Langer R, Sharp PA. Global microRNA depletion suppresses tumor angiogenesis. Genes & Development. 2014. 8. Konermann S*, Brigham MD*, Trevino AE, Hsu PD, Heidenreich M, Cong L, Platt RJ, Scott DA, Church GM, Zhang F. Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013. 9. Cong L*, Ran FA*, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F.. Multiplex genome engineering using CRISPR/Cas systems. Science. 2013. (* Equal contribution. Most highly-cited work in CRISPR field) 10. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F. Comprehensive interrogation of natural TALE DNA- binding modules and transcriptional repressor domains. Nature Communications. 2012. 11. Zhang F, Cong L, Stuber G, Adamantidis A, and Deisseroth K. Analysis of neuronal circuits with optogenetics. 2012. Neuromethods. Springer Science+Business Media, LLC. (Book chapter). 12. Wang HH*, Kim H*, Cong L, Jeong J, Bang D, and Church GM. Genome-scale promoter engineering by co-selection MAGE. Nature Methods. 2012. (* Equal contribution) 13. Sanjana N*, Cong L*, Zhou Y*, Cunniff M, Feng G, and Zhang F. A TAL effector toolbox for genome engineering. Nature Protocols. 2012. (* Equal contribution) 14. Zhang F*, Cong L*, Lodato S, Kosuri S, Church GM, Arlotta P. Efficient construction of sequence- specific TAL effectors for modulating mammalian transcription. Nature Biotechnology. 2011. (* Equal contribution) 15. Xu Y, Cong L, Chen C, Wei L, Zhao Q, Xu X, Ma Y, Bartlam M, and Rao Z. A systematic structural analysis of the viral ADRP domain. Journal of Virology. 2009. 16. Wei L, Chen C, Zhao Q, Li C, Cong L, Xu X, Ma Y, Liao M, Xu Y, Rao Z. Acta Crystallography Section F Structural Biology and Crystallization Communications. 2008.

SELECTED GRANTED PATENTS 1. Zhang, Cong, Platt, Sanjana. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains. US Patent 8,999,641. 2. Cong, Zhang, Hsu, Ran. Engineering of systems, methods and optimized guide compositions for sequence manipulation. EU Patent EP2784162 B1. 3. Zhang, Cong, R Platt, N Sanjana, R Fei. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains. US Patent 8,993,233. 4. Cong, Zhang. CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes. US Patent 8,932,814. 5. Zhang, Cong, Hsu P, Ran F. Engineering of systems, methods and optimized guide compositions for sequence manipulation. US Patent 8,906,616. 6. Cong, Zhang. CRISPR-Cas component systems, methods and compositions for sequence manipulation. US Patent 8,871,445.

FELLOWSHIPS, AWARDS, AND HONORS l Life Science Research Foundation (LSRF) Fellowship Finalist (gratefully withdrawn) 2016 l CRI Irvington Postdoctoral Fellowships Award. Cancer Research Institute (CRI). 2015-Present l Outstanding Presentation Award and Young Scientist Travel Award, The 17th American Society for Gene and Cell Therapy Annual Meeting. Washington D.C. 2014 l Extraordinary Excellence Award, Chinese Government Awards for Students Abroad. 2014 l Howard Hughes Medical Institute (HHMI) International Student Fellowship 2011-2013 l Young Researcher Travel Award. BioBricks Foundation, London, UK. 2013 l Distinguished Life Science Research Award, Harvard Medical School Chinese Scientists and Scholars Associations 2012 l The Grand Scholarship, Tsinghua University (1/13,915) 2009 The highest honor award granted to no more than 5 students out of all 13,915 undergraduates l National Scholarship, the Ministry of Education (MOE) of China 2008 The most prestigious scholarship awarded to top 0.5% college students by the MOE of China

INVITED TALKS AND CONFERENCE PRESENTATIONS l Invited Seminar. Gladstone Institute/UCSF Convergence Seminars. San Francisco, CA. 2015. l Invited Presentation. 2nd Annual Conference on New Frontiers in Gene Editing - Applications and Technologies For Use From the Lab to the Clinic. Boston, MA. 2015. l Invited Talk. Gordon Research Conference. Sunday River, ME. 2015. l Special Workshop Presentation. European Human Genetics Conference 2015. UK. 2015. l Presentation for Presidential Special Program. Japanese Association for RNA Interference (JARI) - Japanese Society for Extracellular Vesicles (JSEV) Joint Annual Meeting. Japan. 2014. l Invited Talk. Plant Genome Stability and Change Conference. Asilomar Conference Grounds. 2014. l Invited Presentation. FASEB Science Research Conferences. Genome Engineering - Cutting- Edge Research and Applications. Bahamas. 2014. l Invited Talk. Canadian Cancer Society & Dana-Farber Cancer Institute Joint Cancer Research Conference. Boston, MA. 2014. l Invited Talk. CRISPR Symposium. Harvard Medical School. Boston, MA. 2013. l Invited Talk. SB6 International Conference on Synthetic Biology. London, UK. 2013. l Invited Presentation. MIT Synthetic Biology Center Annual Symposium. Cambridge, MA. 2013. l Invited Talk. Cold Spring Harbor Asia Conference. Suzhou, China. 2012.

SELECTED TEACHING EXPERIENCE l Invited Lecturer. CSHL course on Advanced Techniques in Molecular Neuroscience (ATMN2015). Cold Spring Harbor Laboratory. 2015. l Guest Lecturer. MIT 9.26J, Principles and Applications of Genetic Engineering for Biotechnology and Neuroscience. Massachusetts Institute of Technology. 2014-2015. l Teaching Fellow and Section Leader. BCMP 200: Molecular Biology. Harvard Medical School. 2010.