Study on Inter-Taxon Population Structure and Diversity Variation Of
Annals of Agricultural Sciences xxx (xxxx) xxx–xxx HOSTED BY Contents lists available at ScienceDirect Annals of Agricultural Sciences journal homepage: www.elsevier.com/locate/aoas Study on inter-taxon population structure and diversity variation of hosta inferring from trnG-trnS regional cpDNA ⁎ Hasan Mehraja,c, , Subarna Sharmaa,c, Kouhei Ohnishib, Kazuhiko Shimasakic a The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8556, Japan b Research Institute of Molecular Genetics, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan c Laboratory of Vegetable and Floricultural Science, Faculty of Agriculture and Marine Science, Kochi University, Kochi 783-8502, Japan ARTICLE INFO ABSTRACT Keywords: Diversity studies are now a key tool in genetic conservation work. The perennial herb genus Hosta showed a Giboshi complex radiation of numerous species in mountains and riversides around the Shikoku Island, Japan. The Plantain lily purpose of this study was to evaluate trnS-trnG regional genetic structure and the genetic divergence within hosta Shikoku Island taxa populations in this area. We sequenced trnS-trnG regional (chloroplast DNA) cpDNA in 81 populations Taxa comprising 399 individuals of 11 Hosta taxa collected from Shikoku area. The different numbers of haplotypes Haplotype network were found in different taxon. The divergences of population of Hosta genus and individual taxon were shown in Phylogenetic tree NJ phylogenetic tree. The highest number of haplotypes (14) was found in H. longipes var. gracillima. H. sie- boldiana and H. kiyosumiensis populations showed the maximum haplotype diversity (1.0), and H. alismifolia showed maximum nucleotide diversity (π: 0.012). The genetic structures of the H.
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