Oral Rehydration of Adult Cattle Using Isotonic Solution of Sugar, Sodium Chloride and Potassium Chloride

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

ABSTRACT Fig 2: Transmission electron photomicrograph of monocyte of dog Present study comprised of 72 crossbred cows (group I= 60 endometritic and group II=12 healthy) at 30±2days postpartum. The showing heterochromatin (a), euchromatin (b), cytoplasmic process (c), polymorphonuclear neutrophils (PMN) cell coun  Vacuole and nuclear membrane. Uranyl acetate and lead citrate × 25500

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

ASSOCIATION OF SEMEN TRAITS IN CONSECUTIVE EJACULATES WITH FSH-β GENE POLYMORPHISM IN HOLSTEIN-FRIESIAN CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

trus synchronizathod that synchronizes ovulations is Corresponding author: [email protected] Fig. 1. Histogram depicting frequency distribution of animal named briefly as “Ovsynch” (Pursley et al., 1995). The right score of respondents Clinical Article study was aimed to evaluate the efficacy of different methods of estrus sync

Fig. 1. Semilogarithmic plot of plasma concentration time profile of amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and i.m. administration in sheep (n=4)

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article 2003) which might lead to increased chances of urolith the time for the urinary tract to restore patency (Parrah, Haryana Vet. (March, 2020) 59(SI), 93-95 Short Communication Research Article formation. The increased hospital incidence can also be 2009) in conjugation with supportive treatments like COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING attributed to the proximity of the clinic as well. According to peritoneal lavage, urinary acidifiers and urinary ORAL REHYDRATION OF ADULT CATTLE USING ISOTONIC SOLUTION OF SUGAR, FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS data published by Department ff Soil Science, Haryana, antiseptics. Irrespective of the status of urinary bladder and SODIUM CHLORIDE AND POTASSIUM CHLORIDE 1 2 RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. majority of the soils were under high status (60%) of urethra, all the cases of obstructive urolithiasis were Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female Y.B. JADHAV*, G.R. BHOJNE, N.P. DAKSHINKAR, N.V. KURKURE and M.N. RANGNEKAR Bule bull (Boselaphus tragocamelus) Department of Animal Reproduction, Gynaecology and Obstetrics, available phosphorus (Gyawali et al., 2016). It is observed surgically treated by tube cystostomy using Foleys Department of Clinical Veterinary Medicine, Nagpur Veterinary College, Nagpur-440006, India Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India that the farmers of Haryana do integrated cultivation catheter.Advantages of tube cystostomy include lower Corresponding author: [email protected] Received: 14.06.2018; Accepted: 27.09.2018 Received: 20.03.2019; Accepted: 13.05.2019 combining fodder production and calf rearing. Cultivation in recurrence, preservation of reproductive function of the this phosphorus rich soil can lead to its accumulation in animal, an opportunity for the removal of cystic calculi and SUMMARY 182 ABSTRACT fodder as well. This aspect can be directly correlated to the simplicity of technique. Tube cystotomy is simple, less The study was conducted on 10 clinically dehydrated adult cattle. The fluid requirement and clinical parameters were evaluated and treated The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in increased occurrence of obstructive urolithiasis of phosphate time consuming, helps in early return of disturbed with electrolyte solution prepared by dissolving electrolytes in 20 liters of water. In all cases with dehydration, the changes in the body temperature postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. origin in buffalo calves of Haryana. Reduced incidence from haemato-biochemical parameters, appropriate procedure remained statistically non-significant. The heart rate, pulse rate decreased after rehydration, however, respiration rate was elevated during treatment Keywords: Conception rate, Double PG, Ovsynch protocol and decreased after completion of therapy. The packed cell volume, total erythrocyte count and hemoglobin were reduced significantly after therapy. other districts may be either due to their lower soil and water for male animals intended for breeding and is associated Due to dehydration, there is increased protein concentration, which significantly decreased after rehydration therapy. The serum sodium values were CONTRIBUTORS MAY NOTE mineral profile or due to surgical intervention at the field with lesser complications (Mangotra et al., 2017). at lower level before oral rehydration and reached up to normal levels while serum potassium was within normal range. In dehydrated animals, level itself. With respect to mineral profile in water of REFERENCES chloride levels were observed at lower side of the normal range. After rehydration therapy, chloride levels were increased and reached near normalcy Haryana a higher level of total dissolved solids (132-552) indicating that this therapy is effective. Glucose levels did not differ significantly in dehydrated and rehydrated animals. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Bailey, C.B. (1981). Silica metabolism and silica urolithiasis in than the critical level (<500) and medium level of hardness ruminants: a review. Canadian J. Anim. Sci. 61: 219-235. Keywords: Adult Cattle, Isotonic solution, Oral rehydration engaged in Veterinary Profession. (Calcium Carbonate 50-180) within the critical limit (<300) Bayoumi, Y.H. and Attia, N.E. (2017). Comparative study of Many diseases of animals cause dehydration (serum protein, sodium, potassium, calcium, chloride and The estrus synchronizathod that synchronizes in the ground water source was observed during the study. obstructive urolithiasis and its sequlae in buffalo calves. Vet. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript World. 10(2): 156-162. including ruminal and enteric diseases, acute mastitis, glucose) and hematological indices (PCV, Hb and TEC) ovulations is named briefly as “Ovsynch” (Pursley et al., Most of the owners mainly depend on ground water sources acute metritis, milk fever and ketosis. Fluid therapy is the were also recorded as per the standard methods. Oral writing/submission. directly or indirectly through a tap water to meet the water Fazili, M.R. and Ansari, M.M. (2007). Prevalence of bovine obstructive 1995). The study was aimed to evaluate the efficacy of urolithiasis in Kashmir valley. Indian Vet. J. 84: 540-541. most important step to save dehydrated animal. However, electrolyte solution was prepared by dissolving Sugar (100 requirement of the animals. Based on this study it can be THE HARYANA VETERINARIAN different methods of estrus synchronisation protocols in l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Gasthuys, F., Steenhaut, M., Moor, A. de, Sercu, K. and DeMoor, A. (1993). the fluid requirement for large animal is very high and g), NaCl (140 g) and KCl (25 g) in 20L of water (Jadhav, crossbred postpartum cows. assumed that high TDS and Calcium carbonate level might Surgical treatment of urethral obstruction due to urolithiasis in difficult to achieve at field conditions. Besides, it is very 2015). The weight of the animal was calculated on the Veterinary Sciences, along with each article. act as a predisposing factor for urolithiasis. male cattle: a review of 85 cases. Vet. Rec. 133(21): 522-526. MATERIAL AND METHODS time consuming and expensive. Therefore, it is rarely basis of formula described in official gazette of Prevention Gutierrez, C., Escolar, E., Juste, M. C., Palacios, M.P. and Corbera, J.A. Editors/Editorial Board Members are highly thankful to l After revision, please return the revised manuscript and rebuttal at the earliest. Treatment of obstructive urolithiasis is primarily A total of 20 healthy crossbred cows at 60-90 days (2000). Severe urolithiasis due to trimagnesiu morthrophosphate adopted for large animals under field level. However, it is of cruelty to draught and pack animal rules, Government of surgical and has been found to vary depending upon calculi in a goat. Vet. Rec. 18: 534. imperative to give fluid therapy in certain clinical India (1965). all the distinguished referees who helped us in the l Please mention your article reference number in all correspondence for a quick clinical status of the animal and duration of obstruction 2 Gyawali, C., Dahiya, D.S., Devaraj, Bhat, M.A. and Bhandari, R.R. situations and cannot be avoided. Since most dehydrated Length (cm) × Girth (cm) response. (Larson, 1996). The different surgical interventions (2016). Spatial distribution of physico-chemical properties and cattle have a metabolic alkalosis, it is necessary to use a Weight of animal (Kg) = evaluation of articles. We request them to continue to employed for the management of obstructive urolithiasis macronutrients in rice growing soils of Haryana. The Ecoscan. non-alkalinizing oral electrolyte solution that does not 10838 l We solicit your co-operation. 10(3&4): 365-370. in bovines are aimed either at urolith removal for normal contain bicarbonate, acetate or propionate (Smith, 2005). Fluid deficit in liters was calculating using formula extend their co-operation and be prompt in future to give Kushwaha, R.B., Amarpal, Aithal, H.P., Kinjavdekar, P. and Pawde, l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, urine flow establishment or for urinary diversion to allow A.M. (2014). Clinical appraisal of 48 cases of obstructive Oral administration can be done by using a full length as mentioned below (Kopcha, 2008). Department of Veterinary Public Health and Epidemiology, College of Veterinary Table 3 urolithiasis in buffalo calves treated with tube cystostomy and orogastric tube and pumping in 20 to 50 litre of fluid, Fluid deficit in liters= Body weight (Kg.) × Estimated their valuable comments on the articles for timely urethrotomy. Adv. in Anim. and Vet. Sci. 2(2): 106-110. depending on rumen capacity and degree of dehydration. dehydration percentage. CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. Distribution pattern of cases of urolithiasis in Larson, B.L. (1996). Identifying, treating and preventing bovine Non-chilled water mixed with electrolytes is the preferred THE HARYANA VETERINARIAN urolithiasis. Vet. Med. 91: 366-377. Animals were restrained properly in a travis before publication of the journal. different districts fluid of choice and will replace the need for intravenous Editors Mangotra, V., Singh, K and Proch, A. (2017). Tube cystostomy in male oral therapy. Orogastric tube was lubricated with glycerine l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Districts Number of Animals Percentage isotonic fluids. Thus, oral hydration through a pump buffalo calves (Bubalis bubalis) suffering from retention of and held gently over the base of the tongue and passed into Editors/Editorial Board Members are highly thankful to engaged in Veterinary Profession. with obstructive (%) urine. J. Anim. Res. 7(2): 279-291. system is the easiest way to rehydrate the cow under field the esophagus and finally in the rumen taking necessary urolithiasis Mann, M.E., Rutherford, S., Bradley, R.S., Hughes, M.K. and Keimig, level to provide important nutrients like water and precautions. The eructation of gases was indicative of all the distinguished referees who helped us in the l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript Hisar 58 40.55 F.T. (2003). Optimal surface temperature reconstructions using electrolytes. The present study provides its detailed Bhiwani 29 20.27 terrestrial borehole data. J. Geophysical Res. 108(D7): 25-32. proper placement of tube in the rumen. The electrolyte writing/submission. nd outcome in dehydrated animals. THE HARYANA VETERINARIAN Jind 17 11.88 Matthews, J. (1999). Diseases of the Goat. (2 Edn.) Blackwell Science, solution from rehydration unit was placed in rumen by evaluation of articles. We request them to continue to This study was conducted to evaluate the effect of l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Fatehabad 14 9.79 London. water pump slowly. During the course of therapy, tube was Veterinary Sciences, along with each article. Churu (Rajasthan) 6 4.19 Monoghan, M.L. and Boy, M.G. (1990). Ruminants Renal System: Diseases oral rehydration therapy on dehydrated adult cattle. For moved forward and backward direction for expulsion of extend their co-operation and be prompt in future to give Editors/Editorial Board Members are highly thankful to Kaithal 6 4.19 of the Renal System. In: Large Animal Internal Medicine. B.P. this purpose, 10 cattle with dehydration were selected from gases from rumen. After drenching electrolyte solution in Smith, (Edt.) C.V. Mosby Company, Philadelphia. Toronto. pp. Gorakshan Sabha, Dhantoli, Nagpur. Animal’s l After revision, please return the revised manuscript and rebuttal at the earliest. Jhunjhunu (Rajasthan) 4 2.79 888-890. the rumen, tube was removed slowly. their valuable comments on the articles for timely dehydration was monitored through skin tent test, eye all the distinguished referees who helped us in the Sonipat 2 1.3 Parrah, J.D. (2009). Clinical Studies on obstructive urolithiasis in l Please mention your article reference number in all correspondence for a quick Dehydrated adult animals were dull, depressed and Rohtak 1 0.69 Cattle. M.V.Sc. thesis submitted to Sher-e-Kashmir University position and mucus membrane characteristics as explained publication of the journal. th th th recumbent. Sticky mucus membrane of eye, sunken eyes evaluation of articles. We request them to continue to response. Mahendragarh 2 1.3 of Agricultural Sciences &Technology of Kashmir, India. by Kopcha (2008) at 0 , 24 and 48 h of therapy. Rewari 1 0.69 and long standing skin ridge was observed in the Radostits, O.M., Blood, D.C., Gay, C.C. and Hinchcliff, K.W. (2000). The other parameters like body temperature, pulse, l We solicit your co-operation. Charkhi Dadri 1 0.69 Veterinary Medicine In: Large Animal Internal Medicine. B.P. dehydrated animals. Constable et al. (1998) suggested a extend their co-operation and be prompt in future to give CONTRIBUTORS MAY NOTE respiration rate, gait of animal, appetite, biochemical Sirsa 1 0.69 Smith, (Edt.) C.V. Mosby Company, Philadelphia. Toronto. pp. complete examination of eye position, skin elasticity and Corresponding author: [email protected] l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, 888-890. Sangrur (Punjab) 1 0.69 *Corresponding author: [email protected] eye mucus membrane moistness in dehydrated animals. their valuable comments on the articles for timely Department of Veterinary Public Health and Epidemiology, Collegel Research/Clinical of Veterinary articles are invited for next issue from the Scientists/Veterinarians publication of the journal. Sciences, LUVAS, Hisar-125004. engaged in Veterinary Profession. 92 93 l Please followEditors strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. THE HARYANA VETERINARIAN l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick Editors/Editorial Board Members are highly thankful to response. all the distinguished referees who helped us in the l We solicit your co-operation. evaluation of articles. We request them to continue to l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary extend their co-operation and be prompt in future to give Sciences, LUVAS, Hisar-125004. their valuable comments on the articles for timely Editors publication of the journal.

THE HARYANA VETERINARIAN THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the Editors/Editorial Board Members are highly thankful to evaluation of articles. We request them to continue to all the distinguished referees who helped us in the extend their co-operation and be prompt in future to give evaluation of articles. We request them to continue to their valuable comments on the articles for timely extend their co-operation and be prompt in future to give publication of the journal. their valuable comments on the articles for timely publication of the journal. THE HARYANA VETERINARIAN Editors/Editorial Board Members are highly thankful to all the distinguished referees who helped us in the 39 CONTRIBUTORS MAY NOTE evaluation of articles. We request them to continue to extend their co-operation and be prompt in future to give l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians engaged in Veterinary Profession. their valuable comments on the articles for timely l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript publication of the journal. writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, LUVAS, Hisar-125004. Editors Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

ASSOCIATION OF SEMEN TRAITS IN CONSECUTIVE EJACULATES WITH FSH-β GENE POLYMORPHISM IN HOLSTEIN-FRIESIAN CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

Fig. 1. Semilogarithmic plot of plasma concentration time profile of amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and i.m. administration in sheep (n=4)

Haryana Vet. (Dec., 2019) 58(2), 166-169 Research Article Table 1 animals showed normal feeding behavior. Kumar and dehydrated animals. Average pulse rate and heart rate of dehydrated cattle with continuous flow system. Research Article th Skin tent test, % dehydration and fluid given Mandial (2002) also reported sunken eyes, reduced skin these animals was 73.1 per minute at 0 hour, which was Thus, this study showed that oral rehydration should COMPARATIVE EFFICACY OF SYNCHRONIZATION PROTOCOLS FOR IMPROVING th th Animal no. Body Skin tent test (seconds) % of fluid elasticity, depression, dullness and reduced appetite in reduced to 65.8 and 62.4 per minute at 24 and 48 hour be done in dehydrated animals after assessing skin FERTILITY IN POSTPARTUM CROSSBRED DAIRY COWS dehydrated animals. after initiation of oral rehydration therapy (Table 2). Boyd 1 2 Weight dehyd- given elasticity and different biochemical and hematological RATNAPARKHI A.R., DESHMUKH S.G.*, BIRADE H.S., KALE V.B., HARKAL S.B. and JADHAO A.D. (kg) 0h 24h 48h ration (L) et al. (1974) also reported normal temperature and pulse Fig. 1. Lateral view of ribs (13 numbers) of left side of adult female Skin tent test was done by pinching the skin at neck indices. Bule bull (Boselaphus tragocamelus) Department of Animal Reproduction, Gynaecology and Obstetrics, rate in dehydrated animals. However, Tennant et al. (1975) 1 191 6 3 0 10 19.1 region of dehydrated animals and time required for the skin REFERENCES Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India observed increased temperature and pulse rate in Corresponding author: [email protected] 2 180 6 2 0 10 18.0 fold to disappear was recorded. The readings of skin tent Alone, S.A., Kolte, A.Y., Sadekar, R.D., Mode, S.G. and Joshi, M.V. Received: 20.03.2019; Accepted: 13.05.2019 dehydrated calves. This may be due to variation in the age 3 247 15 3 3 10 24.7 test and per cent dehydration are closely related to each (2000). Comparative efficacy of different rehydration therapies 182 other as the skin elasticity is lost due to loss of fluid from of the animals. The average respiration rate of animals was in restoring electrolyte imbalances in diarrheic dehydration. ABSTRACT 4 191 7 4 3 10 19.1 th the body. From table 1, skin tent test values shows that skin 21.4 per minute at 0 hour which reduced to 20.6 and 21.6 Indian Vet. J. 77: 124-126. The objective of present research work was to study the efficacy of two different synchronization protocols for improving the fertility in 5 247 10 5 4 10 24.7 th th Avanza, M.F.B. (2004). Enteral fluid in normal and experimentally postpartum crossbred cows. A totah protocol induces better estrus response and first service conception rate in postpartum cows. returned to normal position after 8.70 seconds before oral per minute at 24 and 48 hours after initiation of oral 6 255 6 2 0 10 25.5 rehydration therapy. Similar observations were also dehydrated cows. Combravet, Sau Luis, Maranhaosao Luis Keywords: Conception rate, Double PG, Ovsynch protocol rehydration therapy in severally dehydrated animals which Proceedings. pp. 31-34. 7 210 9 5 3 10 21.0 reported by Boyd et al. (1974) who reported normal heart was further improved and skin came to normal position Benjamin, M.M. (1981). Outline of Veterinary Clinical Pathology (3rd CONTRIBUTORS MAY NOTE 8 188 10 6 4 10 18.8 th th rates and respiration rates in dehydrated animals. after 3.9 and 2.4 seconds at 24 and 48 hours after therapy, Edn). The Iowa State University Press. pp. 213-227. 9 195 10 6 4 10 19.5 th respectively. This indicated that the oral rehydration The average packed cell volume was 32.78% at 0 Boyd, J.W., Baker, J. K. and Leyland, A. (1974). Neonatal diarrhea in l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians 10 210 8 3 3 10 21.0 therapy is very effective in dehydrated animals. The body hour which decreased to 25.99% at 24th hour of therapy calves. Vet. Rec. 5: 310-313. engaged in Veterinary Profession. Average 8.7 3.9 2.4 temperature in all dehydrated animals remained normal which was further increased to 23.51 at 48th hour. Similarly Constable, P.D., Thomas E. and Boisrame B. (2001). Comparison of and averaged between 102 to 101.7° F (Table 2). These trend was also observed for total erythrocyte count at 0th two oral electrolyte solutions for the treatment of dehydrated The estrus synchronizathod that synchronizes l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript All experimental animals were recumbent and showing findings corroborates with findings of Constable et al. (4.87), 24th (4.72) and 48th (4.605) hour. The average calves with experimentally-induced diarrhea. Vet. J. 162: 129- ovulations is named briefly as “Ovsynch” (Pursley et al., stumbling gait. The appetite was decreased in two animals 140. writing/submission. th th 1995). The study was aimed to evaluate the efficacy of and three animals were totally anorectic, however, five (2001) who reported unaltered rectal temperature in hemoglobin level was 10.96, 8.51 and 7.92 gm% at 0 , 24 th Constable, P.D., Walker P.G. and Morin D.E. (1998). Clinical and THE HARYANA VETERINARIAN different methods of estrus synchronisation protocols in l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of and 48 hour, respectively. The reduction in the values of laboratory assessment of hydration status of neonatal calves Table 2 th crossbred postpartum cows. PCV, Hb and TEC at 24 hour after therapy indicated that with diarrhea. J. Am. Vet. Med. Assoc. 212: 991-996. Veterinary Sciences, along with each article. Clinical Parameters th MATERIAL AND METHODS the oral rehydration is required to be continued at 48 hour Dalton, R.G., Fisher E.W. and McIntyre W.I.M. (1965). Changes in Editors/Editorial Board Members are highly thankful to l After revision, please return the revised manuscript and rebuttal at the earliest. Animal No. 1 2 3 4 5 6 7 8 9 10 Average also. Similar trend was also reported by Benjamin (1981). blood chemistry, body weight and haematocrit of calves affected A total of 20 healthy crossbred cows at 60-90 days with neonatal diarrhea. British Vet. J. 121: 34-41. Mucus membrane 0 hr DS DS DS DT DT DT DS DS DT DT The average serum protein level for dehydrated all the distinguished referees who helped us in the l Please mention your article reference number in all correspondence for a quick Government of India (1965). In: Prevention of Cruelty to Drought & 24 hr DT DT DT M DT M DT DT DT DT th animals was 6.15 gm/dl at 0 hour which was reduced to Pack Animal Rules. 1965. response. 48 hr M M DT M M M DT DT DT DT th th evaluation of articles. We request them to continue to 5.66 and 5.22 gm/dl at 24 and 48 hour after therapy. The Jadhav, Y.B. (2015). Comparative evaluation of oral rehydration l We solicit your co-operation. Eye position 0 hr S Res Ob Sun Sv Res Ob Sun Sv Rs Ob Sun Sv Res Sv Res Sv Res Sv Res reduced serum protein value after oral rehydration therapy therapy in adult cattle. M.V.Sc. Thesis submitted to Maharshtra extend their co-operation and be prompt in future to give 24 hr S Res S Res Ob Sun S Res Ob Sun S Res Ob Sun Ob Sun Ob Sun S Res indicated that this therapy is very effective in correction of Animal and Fishery Sciences University, Nagpur. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, 48 hr No Res No Res S Res S Res S Res No Res S Res S Res S Res S Res dehydration. Similar observations were also reported by Kopcha, M. (2008). Oral fluid therapy for adult dairy cattle. Michigan their valuable comments on the articles for timely Department of Veterinary Public Health and Epidemiology, College of Veterinary Avanza (2004) and Benjamin (1981) who reported similar Dairy Review. 10: 1-4.https://msu.edu/~mdr/reprints/April08/ oralfluidreprintapr08.pdf. Accessed on dt. 11/04/2015. CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. Gait 0 hr STU REC STU REC STF STU REC STF STF STU trend in dehydrated animals. The average value of serum THE HARYANA VETERINARIAN 24 hr N STF STU STF STU STU STF STU STU N th Kumar, R. and Mandial, R.K. (2002).Clinico-biochemical and sodium at 0 hour was 139.33 mmol/L which was elevated publication of the journal. therapeutic studies on clinical colibacillosis in cross bred calves. Editors 48 hr N STU N STU N N STU N N N th l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians to 139.69 mmol/L at 24 hour after initiation of therapy. Ind. Vet. J. 79(7): 672-676. th Editors/Editorial Board Members are highly thankful to engaged in Veterinary Profession. Appetite 0 hr DA AN DA N N AN N N N DA This level was slightly reduced to 138.77 mmol/L at 48 Radostits, O.M., Gay C.C., Hinchcliff, K.W. and Constable P.D. (2007). 24 hr N AN N N N AN DA N N N hours which indicated that this therapy should be Disturbances of free water, electrolytes and acid base balance. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript 48 hr N DA N N N DA N N N N continued at 24th and 48th hours also. The increase in the In: Veterinary medicine - a textbook of diseases of cattle, horses, all the distinguished referees who helped us in the th sheep, pigs and goats (10 Edn.), Saunders (Elsevier) writing/submission. Body Temperature (°F) 0 hr 100.6 101.2 102.2 103.1 100.3 99.8 101.8 99.4 99.4 104.1 101.2 values of serum Sodium is indicative of rehydration in the THE HARYANA VETERINARIAN publication. pp. 73-102. evaluation of articles. We request them to continue to 24 hr 101.0 102.8 101.1 102.2 101.0 100.8 100.9 100.0 100.0 102.5 101.2 animals. These findings are similar that of Dalton et. al. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Ribeiro, Filho J.D., Avanza M.F.B., Baptista Filho L.C.F., Dantas W.de 48 hr 101.8 101.9 102.0 102.3 101.7 101.2 101.6 100.8 101.4 102.0 101.7 (1965) who reported increased serum sodium values after Veterinary Sciences, along with each article. M.F., Lima A. de P. and Gomes C.L.N. (2013). Evaluation of extend their co-operation and be prompt in future to give Editors/Editorial Board Members are highly thankful to rehydration. The average serum chloride value was isotonic electrolyte solution by administered enteral via in Pulse rate (per min.) & 0 hr 108 70 68 60 78 48 64 90 90 55 73.1 th th th 102.61, 109.65 and 108.24 mEq/L at 0 , 24 and 48 hour healthy or cattle dehydrated experimentally. Vet. Anim. Sci. l After revision, please return the revised manuscript and rebuttal at the earliest. Heart Rate (per min.) 24 hr 82 72 60 64 68 55 58 70 70 59 65.8 their valuable comments on the articles for timely of study period, respectively. However, these values are 20(3): 9-16. all the distinguished referees who helped us in the 48 hr 75 63 58 66 69 48 62 63 63 57 62.4 l Please mention your article reference number in all correspondence for a quick within normal physiological range. Similar findings were Smith, G. (2005). Fluid therapy in adult cattle. Proc. North Am. Vet. publication of the journal. evaluation of articles. We request them to continue to response. Respiration Rate 0 hr 20 24 28 16 24 28 19 16 16 23 21.4 also recorded by Alone et al. (2000). The average value of Conf. : 38-39. (per min.) 24 hr 19 24 22 20 22 22 16 21 23 17 20.6 serum calcium was 9.98, 9.69 and 9.70 mg/dl at 0th , 24 th & Tennant, B., Harrold, D. and Reina-Guerra, M. (1975). Hematology of l We solicit your co-operation. 48 hr 18 18 24 24 23 18 23 24 25 19 21.6 th neonatal calf. Response associated with acute enteric infections, extend their co-operation and be prompt in future to give CONTRIBUTORS MAY NOTE 48 hours, respectively. The decreased level of serum gram negative septicemia and experimental endotoxaemia. DS- Dry sticky, DT- Dry tacky, M- Moist, No. Res- No Recession, S Res- Severe Recession, Ob Sun- Obviously Sunken, REC- Recumbent, STU- calcium after oral rehydration was also recorded by l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Cornell Vet. 5: 457-475. Corresponding author: [email protected] Stumbling Gait, STF- Stiff Gait N- Normal, AN- Anorexia, DA- Decreased Appetite, N- Normal Appetite their valuable comments on the articles for timely Department of Veterinary Public Health and Epidemiology, Collegel Research/Clinical of Veterinary articles are invited for next issue from the Scientists/Veterinarians Ribeiro et al. (2013) who performed rehydration in publication of the journal. Sciences, LUVAS, Hisar-125004. engaged in Veterinary Profession. 94 95 l Please followEditors strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick response. l We solicit your co-operation. l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary CONTRIBUTORS MAY NOTE Sciences, LUVAS, Hisar-125004. l Research/Clinical articles are invited for next issue from the Scientists/Veterinarians Editors engaged in Veterinary Profession. l Please follow strictly the format of 'The Haryana Veterinarian' for manuscript writing/submission. l Please pay processing fee of Rs. 1000/- online in the account of Dean, College of Veterinary Sciences, along with each article. THE HARYANA VETERINARIAN l After revision, please return the revised manuscript and rebuttal at the earliest. l Please mention your article reference number in all correspondence for a quick Editors/Editorial Board Members are highly thankful to response. all the distinguished referees who helped us in the l We solicit your co-operation. evaluation of articles. We request them to continue to l All correspondence should be addressed to 'The Editor', Haryana Veterinarian, Department of Veterinary Public Health and Epidemiology, College of Veterinary extend their co-operation and be prompt in future to give Sciences, LUVAS, Hisar-125004. their valuable comments on the articles for timely Editors publication of the journal.

Figure 1: Cyclic conditions for PCR profiling for detection of Salmonella genes

ASSOCIATION OF SEMEN TRAITS IN CONSECUTIVE EJACULATES WITH FSH-β GENE POLYMORPHISM IN HOLSTEIN-FRIESIAN CROSSBRED BULLS FROM INDIA VIJAY KADAM, ABH

Fig. 1. Semilogarithmic plot of plasma concentration time profile of amoxicillin and cloxacillin following single dose (10 mg/kg) i.v. and i.m. administration in sheep (n=4)