*REVISEDView metadata, Manuscript citation and (textsimilar UNmarked) papers at core.ac.uk brought to you by CORE Click here to view linked References provided by EPrints Complutense 1 The behaviour of sea anemone actinoporins at the water-membrane interface. Lucía García-Ortega1, Jorge Alegre-Cebollada1,2, Sara García-Linares1, Marta Bruix3, Álvaro Martínez-del-Pozo1,* and José G. Gavilanes1, * 1Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, 28040 Madrid, Spain. 2Present address: Department of Biological Sciences, Columbia University, 1212 Amsterdam Ave., New York, NY 10027, USA. 3Instituto de Química-Física Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain. *To whom correspondence can be addressed: AMP (
[email protected]) and JGG (
[email protected]) Keywords: actinoporin, equinatoxin, sticholysin, membrane-pore, pore-forming-toxin Abbreviations: Avt, actinoporins from Actineria villosa; ALP, actinoporin-like protein; ATR, attenuated total reflection; Bc2, actinoporin from Bunodosoma caissarum; CD, circular dichroism; Chol, cholesterol; DMPC, dimyristoylphosphatidylcholine; DOPC, dioleylphosphatidylcholine; DPC, dodecylphosphocholine; DrI, ALP from Danio rerio; EM, electron microscopy; Ent, actinoporin from Entacmea quadricolor; Eqt, equinatoxin; FTIR, Fourier transform infrared spectroscopy, Fra C, actinoporin from Actinia fragacea; GUV, giant unilamellar vesicles; ITC, isothermal titration calorimetry; NLP, necrosis and ethylene-inducing peptide 1 (Nep1)-like protein; NMR, nuclear magnetic resonance; PE, phosphatidylethanolamine; PFT, pore forming toxin; PpBP, ALP from Physcomitrella patens; Pstx, actinoporins from Phyllodiscus semoni; SM, sphingomyelin; SPR, surface plasmon resonance; Stn, sticholysin; TFE, trifluoroethanol. 2 Abstract Actinoporins constitute a group of small and basic α-pore forming toxins produced by sea anemones. They display high sequence identity and appear as multigene families.