Panoply™ Human GUCY2C Over-Expressing Stable Cell Line

Panoply™ Human GUCY2C Over-expressing Stable Cell Line

HEK293-GUCY2C Lot. No. (See product label)

CELL LINE INFORMATION

Cat.No. CSC-SC006776 Cell Line Description HEK293-GUCY2C cell line is clonally derived from HEK293 cell line, which has been transduced with a lentiviral vector encoding: 1) full length human GUCY2C under the CMV promoter, and 2) puromycin resistance gene under the control of PGK promoter. Overexpression of human GUCY2C has been quantified by qPCR. Background GUCY2C (guanylate cyclase 2C) is an enzyme that in humans is encoded by the GUCY2C gene. It is a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. This protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive). Host Cell HEK293

Gene Information

Gene Name Guanylate cyclase 2C Official Symbol GUCY2C Species Homo sapiens (human) GeneID 2984 mRNA Reference NM_004963.3 Protein Reference NP_004954.2 Synonyms GC-C; STAR; DIAR6; GUC2C; MECIL; MUCIL

SAFETY AND PACKAGING

Mycoplasma Mycoplasma Status: Negative (MycoAlert Kit) Format One frozen vial Storage Liquid nitrogen Safety Considerations The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice

Representative Data

Figure 1. Cell morphology under white light

Human GUCY2C mRNA Over-expression Level (2-ddCt) 70000

60000

50000

40000

30000

20000

10000

Fold increases in number in increases Fold 0 HEK293-hGUCY2C-Monoclonal-2 HEK293-hGUCY2C-Monoclonal-1 HEK293-Puro (Control) Samples

Figure 2. Human GUCY2C qPCR data