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BSE Cases in Department of Food Safety, MHLW Date of Confirmation Date of Birth Cattle Breed Birthplace Institute of Screening test (Date of Slaughter or Result of Confirmatory test (Cattle Age in Month) (Sex) (Producing place) (Institute of Confirmatory test) Dead) Western-blotting + Sep 10, 2001 (*1) Mar 26, 1996 Holstein Prefecture Prefecture Gov. Immunohistochemistry + 1 (Aug 6, 2001) (64 months) (cow) () (National Institute of Animal Health) Histopathology + Western-blotting + Nov 21, 2001 Apr 4, 1996 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 2 (Nov 19, 2001) (67 months) (cow) (Hokkaido Prefecture) ( Univ. of Agriculture and Veterinary Medicine) Histopathology - Prefecture Gov. Western-blotting + Dec 2, 2001 Mar 26, 1996 Holstein Quarantine Station, Immunohistochemistry + 3 (Nov 29, 2001) (68 months) (cow) (Gunma Prefecture) Obihiro Univ. of Agricultureand Veterinary Medicine) Histopathology + Western-blotting + May 13, 2002 Mar 23, 1996 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 4 (May 10, 2002) (73 months) (cow) (Hokkaido Prefecture) (Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology + Western-blotting + Aug 2, 2002 Dec 5, 1997 Holstein Kanagawa Prefecture Gov. Immunohistochemistry + 5 (Aug 21, 2002) (80 months) (cow) (Kanagawa Prefecture) (National Institute of Infectious Diseases) Histopathology - Western-blotting + Jan 20, 2003 Feb 10, 1996 Holstein Hokkaido Prefecture City Gov. Immunohistochemistry + 6 (Jan 17, 2003) (83 months) (cow) () (National Institute of Infectious Diseases) Histopathology + Western-blotting + Jan 23, 2003 Mar 28, 1996 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 7 (Jan 21, 2003) (81 months) (cow) (Hokkaido Prefecture) (Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology - Western-blotting + (*2) Oct 6, 2003 Oct 13, 2001 Holstein Prefecture Gov. Immunohistochemistry - 8 (Sep 29, 2003) (23 months) (bullock) () (National Institute of Infectious Diseases) Histopathology - Western-blotting + Nov 4, 2003 Jan 13, 2002 Holstein Hyogo Prefecture Hukuyama City Gov. Immunohistochemistry - 9 (Oct 29, 2003) (21 months) (bullock) ( Prefecture) (National Institute of Infectious Diseases) Histopathology - Western-blotting + Feb 22, 2004 Mar 17, 1996 Holstein Kanagawa Prefecture Kanagawa Prefecture Gov. Immunohistochemistry + 10 (Feb 20, 2004) (95 months) (cow) (Kanagawa Prefecture) (National Institute of Infectious Diseases) Histopathology + Western-blotting + Mar 9, 2004 (*3) Apr 8, 1996 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. 11 (Mar 4, 2004) (94 months) (cow) (Hokkaido Prefecture) (National Institute of Animal Health) Immunohistochemistry + Histopathology + Western-blotting + Sep 13, 2004 Jul 3, 1999 Holstein Prefecture Gov. 12 (Sep 10, 2004) (62 months) (cow) (Kumamoto Prefecture) (National Institute of Infectious Diseases) Immunohistochemistry + Histopathology + Western-blotting + Sep 23, 2004 Feb 18, 1996 Holstein Hokkaido Prefecture Prefecture Gov. 13 (Sep 21,2004) (103 months) (cow) () (National Institute of Infectious Diseases) Immunohistochemistry + Histopathology + Western-blotting + Oct 14, 2004 (*3) Oct 8, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. 14 (Oct 8, 2004) (48 months) (cow) (Hokkaido Prefecture) (National Institute of Animal Health) Immunohistochemistry + Histopathology + Western-blotting + Feb 26, 2005 (*3) Aug 5, 1996 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. 15 (Feb 22, 2005) (102 months) (cow) (Hokkaido Prefecture) (National Institute of Animal Health) Immunohistochemistry + Histopathology + Asahikawa City Gov. Western-blotting + Mar 27, 2005 Mar 23, 1996 Holstein Hokkaido Prefecture (National Institute of Infectious Diseases, Immunohistochemistry 16 (Mar 24, 2005) (108 months) (cow) (Hokkaido Prefecture) + Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology + Western-blotting + Apr 8, 2005 (*3) Sep 11, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. 17 (Apr 4, 2005) (54 months) (cow) (Hokkaido Prefecture) (National Institute of Animal Health) Immunohistochemistry + Histopathology + Hokkaido Prefecture Gov. Western-blotting + May 12, 2005 Aug 31, 1999 Holstein Hokkaido Prefecture (Hokkaido Univ., Immunohistochemistry + 18 (May 10, 2005) (68 months) (cow) (Hokkaido Prefecture) Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology + Hokkaido Prefecture Gov. Western-blotting + Jun 2, 2005 Apr 16, 1996 Holstein Hokkaido Prefecture (Hokkaido Univ., Immunohistochemistry + 19 (May 31, 2005) (109 months) (cow) (Hokkaido Prefecture) Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology - Western-blotting + Jun 6, 2005 Aug 12, 2000 Holstein Hokkaido Prefecture Ibaraki Prefecture Gov. Immunohistochemistry + 20 (Jun 3, 2005) (57 months) (cow) (Hokkaido Prefecture) (National Institute of Infectious Diseases) Histopathology - Western-blotting + Dec 10, 2005 (*3) Feb 13, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. 21 (Dec 6, 2005) (69 months) (cow) (Hokkaido Prefecture) (National Institute of Animal Health) Immunohistochemistry + Histopathology - Western-blotting + Jan 23, 2006 (*3) Sep 1, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 22 (Jan 20, 1006) (64 months) (cow) (Hokkaido Prefecture) (National Institute of Animal Health) Histopathology (*4) Hokkaido Prefecture Gov. Western-blotting + Mar 15, 2006 Jul 8, 2000 Holstein Hokkaido Prefecture (Hokkaido Univ., Immunohistochemistry + 23 (Mar 13, 2006) (68 months) (cow) (Hokkaido Prefecture) Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology + Western-blotting +(*5) Mar 17, 2006 Feb 10, 1992 Wagyu Prefecture Sasebo City Gov. Immunohistochemistry + 24 (Mar 13, 2006) (169 months) (cow) () (National Institute of Infectious Diseases) Histopathology + Western-blotting + Apr 19, 2006 Apr 18, 2000 Holstein Hokkaido Prefecture Prefecture Gov. Immunohistochemistry + 25 (Apr 17, 2006) (71 months) (cow) () (National Institute of Infectious Diseases) Histopathology - Western-blotting + May 13, 2006(*3) Aug 11, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 26 (May 11, 2006) (68 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology (*4) Western-blotting + May 19, 2006(*3) Aug 20, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. 27 (May 16, 2006) (68 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Immunohistochemistry + Histopathology + Western-blotting + Aug 11, 2006(*3) Nov 21, 1999 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 28 (Aug 7, 2006) (80 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology - Western-blotting + Sep 28, 2006(*3) Jun 24,2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 29 (Sep 24, 2006) (75 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology + Western-blotting + Nov 13, 2006(*3) Jun 28,2001 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 30 (Nov 8, 2006) (64 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology (*4) Hokkaido Prefecture Gov. Western-blotting + Dec 8, 2006 Nov 12, 1999 Holstein Hokkaido Prefecture (Hokkaido Univ., Immunohistochemistry + 31 (Dec 6, 2006) (84 months) (cow) ( HokkaidoPrefecture) Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology - Hokkaido Prefecture Gov. Western-blotting + Feb 5, 2007 Aug 26, 2001 Holstein Hokkaido Prefecture (Hokkaido Univ., Immunohistochemistry + 32 (Feb 2, 2007) (65 months) (cow) ( HokkaidoPrefecture) Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology + Western-blotting + Jul 2, 2007(*3) Jun 21, 2000 Wagyu Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 33 (Jun 24, 2007) (84 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology (*4) Hokkaido Prefecture Gov. Western-blotting + Dec 21, 2007 Jul 4, 1992 Wagyu (Hokkaido Univ., Immunohistochemistry + 34 (Dec 19, 2007) (185 months) (cow) ( HokkaidoPrefecture) Obihiro Univ. of Agriculture and Veterinary Medicine) Histopathology - Western-blotting + Mar 24, 2008(*3) Oct 12, 2000 Wagyu Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 35 (Mar 17, 2008) (89 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology (*4) Western-blotting + Jan 30, 2009(*3) Aug 5, 2000 Holstein Hokkaido Prefecture Hokkaido Prefecture Gov. Immunohistochemistry + 36 (Jun 26, 2009) (101 months) (cow) ( HokkaidoPrefecture) (National Institute of Animal Health) Histopathology + *1: BSE detected first case in BSE surveillance of MAFF *2: The pattern of glycoform and proteinase K(PK) resistance of the PrP in this case was different from that of confirmed BSE *3: Dead cow at dairy farm *4: Vacuolar degeneration was observed, but it was difficult to evidently distinguish the degeneration from postmortem change *5: The pattern of glycoform of the PrP in this case was different from that of confirmed BSE