1 Integrative analyses unveil speciation linked to host plant shift in 2 Spialia butterflies 3 4 5 JUAN L. HERNÁNDEZ-ROLDÁN1,2*, LEONARDO DAPPORTO1,3*, VLAD DINCĂ 1,4, JUAN C. 6 VICENTE5, EMILY A. HORNETT6, JINDRA ŠÍCHOVÁ7, VLADIMIR LUKHTANOV8,9, GERARD 7 TALAVERA1,10 & ROGER VILA1 8 9 1Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Passeig Marítim de la Barceloneta, 10 37, E-08003 Barcelona, Spain 11 2Departamento de Biología (Zoología), Facultad de Ciencias de la Universidad Autónoma de Madrid, 12 C/ Darwin, 2, E-28049 Madrid, Spain 13 3Department of Biology, University of Florence, Via Madonna del Piano 6, 50019, Sesto Fiorentino 14 (FI), Italy 15 4Biodiversity Institute of Ontario, University of Guelph, Guelph, Ontario, N1G 2W1, Canada 16 5C/ Witerico, 9A - Bajo B, E-28025 Madrid, Spain 17 6Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK 18 7Institute of Entomology, Biology Centre ASCR, České Budějovice, Czech Republic 19 8Department of Karyosystematics, Zoological Institute of Russian Academy of Sciences, St. 20 Petersburg, Russia 21 9Department of Entomology, St. Petersburg State University, St. Petersburg, Russia 22 10Department of Organismic and Evolutionary Biology and Museum of Comparative Zoology, 23 Harvard University, Cambridge, MA 02138, USA 24 25 * These authors contributed equally to this work 26 27 Key words: Biogeography, butterflies, Lepidoptera, new species, phylogeny, speciation 28 29 Correspondence: Roger Vila, Fax: +34 932211011; E-mail:
[email protected] 30 31 Running title: Speciation linked to host plant shift in Spialia 32 33 Abstract 34 Discovering cryptic species in well-studied areas and taxonomic groups can have profound implications in 35 understanding eco-evolutionary processes and in nature conservation because such groups often involve research 36 models and act as flagship taxa for nature management.