12 9 Abstract—The systematic status Systematics of North Pacific sand lances of of North Pacific sand lances (ge- nus Ammodytes) was assessed from the genus Ammodytes based on molecular and mitochondrial DNA (cytochrome oxidase c subunit 1) sequence data morphological evidence, with the description of and morphological data to identify a new species from Japan the number of species in the North Pacific Ocean and its fringing seas. Although only 2 species, Ammodytes James W. Orr (contact author)1 hexapterus and A. personatus, have Sharon Wildes2 been considered valid in the region, Yoshiaki Kai3 haplotype networks and trees con- structed with maximum parsimony Nate Raring1 and genetic distance (neighbor- T. Nakabo4 joining) methods revealed 4 highly Oleg Katugin5 divergent monophyletic clades that 2 clearly represent 4 species of Ammo- Jeff Guyon dytes in the North Pacific region. On the basis of our material and com- Email address for contact author:
[email protected] parisons with sequence data report- ed in online databases, A. personatus 1 Resource Assessment and Conservation 3 Maizuru Fisheries Research Station is found throughout the eastern Engineering Division Field Science Education and Research Center North Pacific Ocean, Gulf of Alaska, Alaska Fisheries Science Center Kyoto University Aleutian Islands, and the eastern National Marine Fisheries Service, NOAA Nagahama, Maizuru Bering Sea where it co-occurs with 7600 Sand Point Way NE Kyoto 625-0086, Japan a northwestern Arctic species, A. Seattle, Washington 98115-6349 4 The